subject:(Knockout mice)

1. Pierce, Marsha Louise. Inner Ear Hair Cell Loss and Stereocilia Defects in miR-183 Family Knockout Models.

Degree: PhD, Biomedical Sciences (graduate program), 2015, Creighton University

► Hearing loss is the most prevalent sensory defect, and although hearing aids and cochlear implants can provide sensory input, no current therapeutic options exist for… (more)

▼ Hearing loss is the most prevalent sensory defect, and although hearing aids and cochlear implants can provide sensory input, no current therapeutic options exist for restoration of normal hearing. The microRNA-183 family (miR-183, miR-96, and miR-182) is highly conserved and coordinately expressed in neurosensory cells including hair cells (HCs) and sensory neurons in the inner ear, and mutations in miR-96 cause deafness in both mice and humans. To specifically investigate the effects of miR-183 family member loss-of-function, miR-183/96 and miR-182 knockout (KO) mice were assessed. Behavioral observations and evaluation of Preyer’s reflex were used to grossly assess hair cell function. Stereocilia defects and hair cell loss in mice ranging from birth (postnatal day zero; P0) to P180 were examined by immunofluorescence microscopy (IFM) detection of MyoVIIa, scanning electron microscopy (SEM), and phase contrast microscopy (PCM) of plastic embedded sections through the organ of Corti. Spiral ganglion innervation was evaluated by IFM detection for acetylated tubulin. Targetscan predicted target genes were compared to neonatal and adult hair cell transcriptomes, genes known to cause hereditary hearing loss, and miR-183 familly confirmed target genes in the literature to identify a relevant subset of genes for further evaluation. Here we show that miR-183/96 KO caused delayed stereocilia development, gross stereocilia disorganization, hair cell loss, innervation defects and deafness. Moreover, miR-183/96 heterozygous (HET) and miR-182 KO mice showed relatively subtle stereocilia defects and age-related loss of acoustic startle response. Arhgdia, Sox2 and Tes were co-expressed with miR-183 family members and mildly upregulated in miR-183/96 KO mice, suggesting such targets might play a role in the observed phenotype. Results demonstrate that miR-183 family loss-of-function (LOF) led to stereocilia defects, hair cell loss, and contributing to hearing deficits. The challenge remains to conclusively identify miR-183 family effects on target genes and pathways that support hair cell maintenance and function to provide insight to approaches for preventing hair cell loss. Advisors/Committee Members: Soukup, Garrett A. (advisor), Pierce, Marsha Louise (cuauthor).

Subjects/Keywords: Hearing Loss; MicroRNAs; Mice, Knockout

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pierce, M. L. (2015). Inner Ear Hair Cell Loss and Stereocilia Defects in miR-183 Family Knockout Models. (Doctoral Dissertation). Creighton University. Retrieved from http://hdl.handle.net/10504/69027

Chicago Manual of Style (16^th Edition):

Pierce, Marsha Louise. “Inner Ear Hair Cell Loss and Stereocilia Defects in miR-183 Family Knockout Models.” 2015. Doctoral Dissertation, Creighton University. Accessed January 18, 2021. http://hdl.handle.net/10504/69027.

MLA Handbook (7^th Edition):

Pierce, Marsha Louise. “Inner Ear Hair Cell Loss and Stereocilia Defects in miR-183 Family Knockout Models.” 2015. Web. 18 Jan 2021.

Vancouver:

Pierce ML. Inner Ear Hair Cell Loss and Stereocilia Defects in miR-183 Family Knockout Models. [Internet] [Doctoral dissertation]. Creighton University; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10504/69027.

Council of Science Editors:

Pierce ML. Inner Ear Hair Cell Loss and Stereocilia Defects in miR-183 Family Knockout Models. [Doctoral Dissertation]. Creighton University; 2015. Available from: http://hdl.handle.net/10504/69027

University of Illinois – Chicago

2. Fagen, Sara M. Analysis of Wound Healing Phenotypes of Genetically Deficient Mice.

Degree: 2017, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/21788

► A large number of publications now document alterations in wound healing phenotypes in genetically deficient or “knockout” mice. These studies have produced a disorganized dataset… (more)

Subjects/Keywords: wound healing; knockout mice; healing phenotype

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APA (6^th Edition):

Fagen, S. M. (2017). Analysis of Wound Healing Phenotypes of Genetically Deficient Mice. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21788

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fagen, Sara M. “Analysis of Wound Healing Phenotypes of Genetically Deficient Mice.” 2017. Thesis, University of Illinois – Chicago. Accessed January 18, 2021. http://hdl.handle.net/10027/21788.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fagen, Sara M. “Analysis of Wound Healing Phenotypes of Genetically Deficient Mice.” 2017. Web. 18 Jan 2021.

Vancouver:

Fagen SM. Analysis of Wound Healing Phenotypes of Genetically Deficient Mice. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10027/21788.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fagen SM. Analysis of Wound Healing Phenotypes of Genetically Deficient Mice. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21788

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Southern California

3. Pang, Raina D. Genotype and sex effects on anxiety behavior and functional activation in corticolimbic circuits in serotonin transporter knockout mice (5-HITT KO).

Degree: PhD, Neuroscience, 2012, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/43601/rec/3027

► In humans, the serotonin transporter linked polymorphic region (5-HTTLPR) results in altered transcriptional efficiency of the transporter gene, as well as uptake of serotonin (5-HT)… (more)

▼ In humans, the serotonin transporter linked polymorphic region (5-HTTLPR) results in altered transcriptional efficiency of the transporter gene, as well as uptake of serotonin (5-HT) by neurons. The low expressing `s' allele of this polymorphism has been linked to increased susceptibility to stress and altered connectivity of corticolimbic neural circuits, the disruption of which is widely invoked to explain emotional dysregulation in anxiety and mood disorders. ❧ Serotonin transporter (5-HTT) knockout (KO) mice offer a promising model for psychiatric research as close parallels exist between the human polymorphism and the mouse model at the level of serotonergic profile, behavior, physiological function, and stress hormone response. Although much work has gone into characterizing 5-HTT KO mice, little work to date has examined functional activation patterns and connectivity of circuits involved in emotional regulation in these mice. The experiments outlined in this dissertation address this gap in knowledge by examining sex and genotypic differences in the underlying functional activation of corticolimbic circuits. The inclusion of females in this study provides important information about how sex differences in serotonergic function alter brain function. Functional connectivity analysis is also undertaken in a combined male/female groups to determine genotypic effects on a network level. ❧ Overall, the experiments contained in this dissertation contribute novel information about the role of sex and 5-HTT genotype in anxiety like behavior and function of corticolimbic circuits. Specifically, this is the first study using whole brain functional perfusion mapping in the 5-HTT KO mouse model. Furthermore, these studies support sex differences in functional regulation of the prefrontal-amygdala network in a fear-conditioned paradigm. These studies establish the translational value of functional brain mapping endpoints and suggest greater specificity than the behavioral endpoints. Future studies could address the underlying molecular and structural changes accompanying functional changes seen in 5-HTT KO mice. Advisors/Committee Members: Holschneider, Daniel P. (Committee Chair), Jakowec, Michael (Committee Member), Celikel, Tansu (Committee Member), Watts, Alan G. (Committee Member).

Subjects/Keywords: serotonin transporter knockout mice; amygdala; functional neuroimaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pang, R. D. (2012). Genotype and sex effects on anxiety behavior and functional activation in corticolimbic circuits in serotonin transporter knockout mice (5-HITT KO). (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/43601/rec/3027

Chicago Manual of Style (16^th Edition):

Pang, Raina D. “Genotype and sex effects on anxiety behavior and functional activation in corticolimbic circuits in serotonin transporter knockout mice (5-HITT KO).” 2012. Doctoral Dissertation, University of Southern California. Accessed January 18, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/43601/rec/3027.

MLA Handbook (7^th Edition):

Pang, Raina D. “Genotype and sex effects on anxiety behavior and functional activation in corticolimbic circuits in serotonin transporter knockout mice (5-HITT KO).” 2012. Web. 18 Jan 2021.

Vancouver:

Pang RD. Genotype and sex effects on anxiety behavior and functional activation in corticolimbic circuits in serotonin transporter knockout mice (5-HITT KO). [Internet] [Doctoral dissertation]. University of Southern California; 2012. [cited 2021 Jan 18]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/43601/rec/3027.

Council of Science Editors:

Pang RD. Genotype and sex effects on anxiety behavior and functional activation in corticolimbic circuits in serotonin transporter knockout mice (5-HITT KO). [Doctoral Dissertation]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/43601/rec/3027

4. Bombo, Renata de Paula Assis. Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica do receptor de LDL.

Degree: PhD, Endocrinologia, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5135/tde-29102014-143321/ ;

►

Introdução: Os fitoesteróis (FE) são reconhecidos por reduzirem a concentração plasmática de LDL-colesterol, sendo importantes coadjuvantes no tratamento da hipercolesterolemia moderada. Entretanto, estudos publicados recentemente… (more)

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Introdução: Os fitoesteróis (FE) são reconhecidos por reduzirem a concentração plasmática de LDL-colesterol, sendo importantes coadjuvantes no tratamento da hipercolesterolemia moderada. Entretanto, estudos publicados recentemente demonstram resultados conflitantes em relação à eficiência dos FE na prevenção da aterosclerose. Além disso, algumas investigações evidenciaram que o aumento da concentração plasmática de FE está positivamente relacionado ao risco de desenvolvimento de aterosclerose. Com a finalidade de elucidar a sua ação sobre esses parâmetros, o objetivo deste estudo foi avaliar os efeitos da suplementação de FE no desenvolvimento da aterosclerose em camundongos com ablação gênica para o receptor de LDL (LDLr-KO). Métodos: Os animais foram alimentados durante 16 semanas, com dieta rica em gordura (40% do valor calórico total da dieta), suplementada (grupo FE; 2%, n=10) ou não (Controle; n=10) com FE. Foram avaliadas as concentrações plasmáticas e hepáticas de colesterol, triglicérides, FE (beta-sitosterol, campesterol e latosterol). Na aorta dos animais, determinaram-se as concentrações de colesterol total, colesterol livre e éster e FE, além do infiltrado de macrófagos e infiltrado de lípides. Nos macrófagos do peritôneo dos animais, os quais assemelham-se aos presentes na artéria, avaliou-se a expressão de RNA mensageiro dos genes envolvidos no efluxo e influxo de colesterol (ABCA1, ABCG1, LOX1 e CD36). Também determinou-se as concentrações de FE no intestino e baço dos animais. Resultados: Conforme esperado, o consumo de FE induziu elevação plasmática dos principais FE, campesterol e de beta-sitosterol, reduzindo a concentração de colesterol no plasma. Houve aumento nas concentrações hepáticas de triglicérides e FE, entretanto, não foram observadas diferenças entre os grupos nas expressões de RNA mensageiro de genes lipolíticos (CPT, PPAR alfa) e lipogênicos (SREBP1-c, MTP, LXR e PPAR gamma) no fígado. Não houve, também, alteração no SREBP2, gene relacionado à síntese de colesterol. O conteúdo de colesterol total na artéria foi menor nos animais do grupo FE, não diferindo entre as formas livre e éster. As concentrações de FE na artéria foram iguais entre os grupos. A área de lesão no grupo FE foi menor em relação ao grupo-controle. A suplementação com FE induziu redução na expressão de RNA mensageiro de ABCG1, não interferindo na expressão dos outros genes estudados na artéria. Conclusão: Os achados deste estudo demonstram que a elevação de FE no plasma não induziu o seu acúmulo na parede da artéria e preveniu o desenvolvimento da aterosclerose

Introduction: The plasma cholesterol-reducing effect of hytosterols (PS) is well recognized and they are considered important adjuncts in the treatment of moderate hypercholesterolemia. However, recent studies have shown conflicting results regarding the efficiency of PS in the prevention of atherosclerosis. In addition, some studies showed that the increase in plasma PS concentration is positively correlated to the risk of atherosclerosis. In order to…

Advisors/Committee Members: Lottenberg, Ana Maria Pita.

Subjects/Keywords: Aterosclerose; Atherosclerosis; Camundongos Knockout; Cholesterol; Colesterol; Diet; Dieta; Fitoesteróis; Hipercolesterolemia; Hypercholesterolemia; Knockout mice; Phytosterols

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bombo, R. d. P. A. (2014). Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica do receptor de LDL. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5135/tde-29102014-143321/ ;

Chicago Manual of Style (16^th Edition):

Bombo, Renata de Paula Assis. “Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica do receptor de LDL.” 2014. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-29102014-143321/ ;.

MLA Handbook (7^th Edition):

Bombo, Renata de Paula Assis. “Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica do receptor de LDL.” 2014. Web. 18 Jan 2021.

Vancouver:

Bombo RdPA. Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica do receptor de LDL. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5135/tde-29102014-143321/ ;.

Council of Science Editors:

Bombo RdPA. Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica do receptor de LDL. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/5/5135/tde-29102014-143321/ ;

5. 張, 三兵; ザン, サンビン. Function of draxin on hippocampal development : カイマハッタツニオケルドラキシンノキノウ; 海馬発達におけるドラキシンの機能.

Degree: Kumamoto University / 熊本大学

URL: http://hdl.handle.net/2298/16731

► The hippocampus plays an essential role in learning and memory and is one of the major sites implicated in neural diseases. The proper organization of… (more)

Subjects/Keywords: Draxin; hippocampus; draxin knockout mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

張, 三兵; ザン, �. (n.d.). Function of draxin on hippocampal development : カイマハッタツニオケルドラキシンノキノウ; 海馬発達におけるドラキシンの機能. (Thesis). Kumamoto University / 熊本大学. Retrieved from http://hdl.handle.net/2298/16731

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

張, 三兵; ザン, サンビン. “Function of draxin on hippocampal development : カイマハッタツニオケルドラキシンノキノウ; 海馬発達におけるドラキシンの機能.” Thesis, Kumamoto University / 熊本大学. Accessed January 18, 2021. http://hdl.handle.net/2298/16731.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

張, 三兵; ザン, サンビン. “Function of draxin on hippocampal development : カイマハッタツニオケルドラキシンノキノウ; 海馬発達におけるドラキシンの機能.” Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

張, 三兵; ザン �. Function of draxin on hippocampal development : カイマハッタツニオケルドラキシンノキノウ; 海馬発達におけるドラキシンの機能. [Internet] [Thesis]. Kumamoto University / 熊本大学; [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2298/16731.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

張, 三兵; ザン �. Function of draxin on hippocampal development : カイマハッタツニオケルドラキシンノキノウ; 海馬発達におけるドラキシンの機能. [Thesis]. Kumamoto University / 熊本大学; Available from: http://hdl.handle.net/2298/16731

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

6. Yadav, Roopali. Structure-Function and Behavioral Studies on the Glutamate Delta-1 Receptor.

Degree: PhD, Pharmacology (graduate program), 2012, Creighton University

URL: http://hdl.handle.net/10504/24966

► The delta family of ionotropic glutamate receptors (iGluRs) consists of glutamate delta-1 (GluD1) and glutamate delta-2 (GluD2) receptors. The function of GluD1 in the central… (more)

▼ The delta family of ionotropic glutamate receptors (iGluRs) consists of glutamate delta-1 (GluD1) and glutamate delta-2 (GluD2) receptors. The function of GluD1 in the central nervous system (CNS) remains elusive. As a first step we conducted electrophysiological studies on the rat clone of GluD1 to study the activation gate and ligand binding domain of GluD1 receptor. Site directed mutagenesis in the SYTANLAAF region resulted in four constitutively open mutants GluD1A650C, GluD1L652A, GluD1A654C, and GluD1F655A. Channel blockers pentamidine and NASP inhibited currents through the GluD1 mutant receptors. D-serine and extracellular calcium had opposing effects on GluD1 mutants and a chimeric GluD1-D2 lurcher. D-serine decreased currents through GluD1F655A and chimeric GluD1-D2 lurcher while calcium increased currents through GluD1F655A. These results suggest, GluD1 receptors have a conserved activation gate. In addition conformational changes brought about by D-serine and calcium are conserved among GluD1 and GluD2 receptors. GluD1 functions in synapse formation. Addtionally the GRID1 gene has been implicated in neuropsychiatric disorders. We hypothesize that synaptic abnormalities due to GluD1 deletion would lead to aberrant behaviors. Thus we performed tests in GluD1 knockout (GluD1 KO) mice to study the functional significance of the GluD1 receptor. GluD1 KO mice showed hyperactivity, lower anxiety-like behavior, hyperaggression, depression-like behavior, and social interaction deficits. Altered levels of synaptic proteins and iGluR subunits were seen in prefrontal cortex and amygdala. Few aberrant behaviors were rescued by chronic lithium and D-cycloserine (DCS) treatment. Amygdala functions in associative fear learning while prefrontal cortex plays a role in working memory. We found molecular abnormalities in amygdala and prefrontal cortex. Thus we tested the effect of GluD1 deletion on amygdalar and prefrontal cortex associated learning tasks. We observed normal or enhanced learning for prefrontal cortex associated tasks. However we found deficits in fear learning. iGluR expression was altered in the hippocampus along with synaptic anomalies in the amygdala, prefrontal cortex and hippocampus. These data suggest GluD1 functions in regulation of behavior and synaptic physiology. In addition GluD1 KO mice manifest symptoms related to neuropsychiatric disorders with which GRID1 has been associated. Advisors/Committee Members: Dravid, Shashank M. (advisor), Yadav, Roopali (cuauthor).

Subjects/Keywords: Receptors, Glutamate – genetics; Behavior, Animal; Mice, Knockout; Mental Disorders – genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yadav, R. (2012). Structure-Function and Behavioral Studies on the Glutamate Delta-1 Receptor. (Doctoral Dissertation). Creighton University. Retrieved from http://hdl.handle.net/10504/24966

Chicago Manual of Style (16^th Edition):

Yadav, Roopali. “Structure-Function and Behavioral Studies on the Glutamate Delta-1 Receptor.” 2012. Doctoral Dissertation, Creighton University. Accessed January 18, 2021. http://hdl.handle.net/10504/24966.

MLA Handbook (7^th Edition):

Yadav, Roopali. “Structure-Function and Behavioral Studies on the Glutamate Delta-1 Receptor.” 2012. Web. 18 Jan 2021.

Vancouver:

Yadav R. Structure-Function and Behavioral Studies on the Glutamate Delta-1 Receptor. [Internet] [Doctoral dissertation]. Creighton University; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10504/24966.

Council of Science Editors:

Yadav R. Structure-Function and Behavioral Studies on the Glutamate Delta-1 Receptor. [Doctoral Dissertation]. Creighton University; 2012. Available from: http://hdl.handle.net/10504/24966

Penn State University

7. Lu, Chen. INITIAL FUNCTIONAL STUDY OF THE PRAME GENE FAMILY DURING SPERMATOGENESIS.

Degree: 2017, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/14484cbl5132

► Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis (CT) antigen that is predominantly expressed in normal gametogenic tissues and a variety of tumors. Members… (more)

▼ Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis (CT) antigen that is predominantly expressed in normal gametogenic tissues and a variety of tumors. Members of PRAME gene family encode leucine-rich repeat (LRR) proteins that provide a versatile structural framework for the formation of protein-protein interactions. The function of PRAME during testicular development and spermatogenesis remains unknown, although PRAME has been extensively studied in cancer biology and is believed to play a regulatory role in cancer cells. To explore the function of the PRAME gene in spermatogenesis, the subcellular localization of the PRAME and PRAMEL1 protein in mouse spermatogenic cells was characterized by immunoelectron microscopy. The results revealed that both PRAME and PRAMEL1 localized in various organelles in spermatogenic cells during spermatogenesis, including the nucleus, flagellum, intermitochondrial cement (IMC), and chromatoid body (CB). Apart from that, the PRAME protein was also detected in mitochondria while PRAMEL1 was observed in manchette. To further explore the function of the PRAME gene during spermatogenesis, we applied a conditional knockout (cKO) mouse model with PRAME gene mutated 3 days after birth. Compared with control mice, the cKO mice have smaller testes and less epididymis sperm at 4-month old (4M). When examining the first wave of spermatogenesis, a degradation of seminiferous tubules (with a dramatically reduce of germ cells) was detected in the testes of cKO mice at postnatal day (P) 21 and P35, although degradation of seminiferous tubules was hardly detected in P14 and P7 testes. Apoptosis was also increased in cKO mice at P7, P14 and P21. Ultrastructure analysis detected heterogeneous chromatin condensing with small and large vacuoles in the elongated spermatids of cKO mice, which was not observed in the control mice. Despite the observed disruptions in seminiferous tubules and spermatogenic cells, the cKO mice exhibited normal fertility as indicated by the pregnancy rate and litter size in mating tests. The results indicate that PRAME is involved in spermatogenesis, and the knockout of PRAME gene 3 days after birth in the mouse could lead to certain testis abnormality without a significant impact on male fertility. Advisors/Committee Members: Wansheng Liuf, Thesis Advisor/Co-Advisor, Francisco Javier Diaz, Committee Member, Troy Ott, Committee Member.

Subjects/Keywords: PRAME/PRAMEL1; immunoelectron microscopy; conditional knockout mice model; spermatogenesis

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APA (6^th Edition):

Lu, C. (2017). INITIAL FUNCTIONAL STUDY OF THE PRAME GENE FAMILY DURING SPERMATOGENESIS. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/14484cbl5132

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lu, Chen. “INITIAL FUNCTIONAL STUDY OF THE PRAME GENE FAMILY DURING SPERMATOGENESIS.” 2017. Thesis, Penn State University. Accessed January 18, 2021. https://submit-etda.libraries.psu.edu/catalog/14484cbl5132.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lu, Chen. “INITIAL FUNCTIONAL STUDY OF THE PRAME GENE FAMILY DURING SPERMATOGENESIS.” 2017. Web. 18 Jan 2021.

Vancouver:

Lu C. INITIAL FUNCTIONAL STUDY OF THE PRAME GENE FAMILY DURING SPERMATOGENESIS. [Internet] [Thesis]. Penn State University; 2017. [cited 2021 Jan 18]. Available from: https://submit-etda.libraries.psu.edu/catalog/14484cbl5132.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lu C. INITIAL FUNCTIONAL STUDY OF THE PRAME GENE FAMILY DURING SPERMATOGENESIS. [Thesis]. Penn State University; 2017. Available from: https://submit-etda.libraries.psu.edu/catalog/14484cbl5132

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

8. Young, Samantha Allison Mary. Generation of gene disrupted mice to further elucidate the reproductive mechanisms of male factor fertility.

Degree: PhD, 2016, University of Newcastle

URL: http://hdl.handle.net/1959.13/1325823

►

Research Doctorate - Doctor of Philosophy (PhD)

The issue of fertility is one of increasing importance in a global context, with infertility on the rise,… (more)

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Research Doctorate - Doctor of Philosophy (PhD)

The issue of fertility is one of increasing importance in a global context, with infertility on the rise, along with increased use of assisted reproductive technologies. Yet our understanding of these often incredibly complex processes is still low, due in part to the difficulty in replicating the in vivo environment of fertilization in an in vitro setting, for any length of time. To counter this, we turn to genetically modified organisms, however, in the past the generation of animal models has been expensive, difficult and time consuming. The adaptation of the CRISPR/Cas9 system of genetic engineering to work in mammalian cells has allowed us to explore more than ever the role of certain genes in reproduction. One such animal model that is of particular use is the mouse. They are genetically similar to humans, allowing us to investigate conserved genes with potentially similar functions. They also have a relatively short reproductive cycle, making them effective for genetic modification, as several generations can be analysed within a short span of time. Herein, we examine the various uses of the CRISPR/Cas9 system in the mouse to target the specific area of male factor fertility. This system has developed dramatically in the few years that it has been available, from being able to generate a total gene knockout to subtler genetic modifications. We begin by utilizing the most basic function of the CRISPR/Cas9 system, generating a total gene knockout. Here, we describe for the first time the in vivo role of a gene previously thought to be linked to capacitation. The calcium-binding tyrosine phosphorylation-regulated protein (CABYR) is known in the literature to bind to the fibrous sheath of sperm flagella, however, by removing Cabyr by CRISPR/Cas9 gene knockout we observed that the role of CABYR is in fact in the correct formation of the fibrous sheath. This discovery adds much to the knowledge of the formation and function of the sperm flagella, perhaps even providing a diagnosis for male factor infertility due to poor sperm motility, which has previously been labelled as unexplained male factor infertility. This thesis also explores the use of CRISPR/Cas9 to delete regions of the genome, as well as performing amino acid substitutions. Whilst the genes chosen for these experiments led to no change in the fertility phenotype, it is the ability of the CRISPR/Cas9 system to perform these genetic modifications that is of great interest. The genes selected for this study were all involved in the formation or function of the dynein complex, the motor system that provides the flagella with the ability to move. The selection of these genes came from proteomic work that indicated phosphorylation changes, indicating a control mechanism for sperm motility. The fact that CRISPR/Cas9 could be used to substitute to potential phosphorylation sites demonstrates how much further we can take the investigation of fertility with the use of this system.…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: CRISPR/Cas9; spermatozoa; mice; male factor fertility; knockout; thesis by publication

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APA (6^th Edition):

Young, S. A. M. (2016). Generation of gene disrupted mice to further elucidate the reproductive mechanisms of male factor fertility. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1325823

Chicago Manual of Style (16^th Edition):

Young, Samantha Allison Mary. “Generation of gene disrupted mice to further elucidate the reproductive mechanisms of male factor fertility.” 2016. Doctoral Dissertation, University of Newcastle. Accessed January 18, 2021. http://hdl.handle.net/1959.13/1325823.

MLA Handbook (7^th Edition):

Young, Samantha Allison Mary. “Generation of gene disrupted mice to further elucidate the reproductive mechanisms of male factor fertility.” 2016. Web. 18 Jan 2021.

Vancouver:

Young SAM. Generation of gene disrupted mice to further elucidate the reproductive mechanisms of male factor fertility. [Internet] [Doctoral dissertation]. University of Newcastle; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1959.13/1325823.

Council of Science Editors:

Young SAM. Generation of gene disrupted mice to further elucidate the reproductive mechanisms of male factor fertility. [Doctoral Dissertation]. University of Newcastle; 2016. Available from: http://hdl.handle.net/1959.13/1325823

University of Manitoba

9. Seif, Samira. Characterization of vascular remodeling associated with pulmonary hypertension in miR-200b knockout mice.

Degree: Physiology and Pathophysiology, 2019, University of Manitoba

URL: http://hdl.handle.net/1993/34446

► Abstract Introduction: Pulmonary hypertension is one of the main causes of death in congenital diaphragmatic hernia. It results from thickening of the medial and adventitial… (more)

▼ Abstract Introduction: Pulmonary hypertension is one of the main causes of death in congenital diaphragmatic hernia. It results from thickening of the medial and adventitial layers in the lung vessels leading to increased vessel resistance and ultimately heart failure. The underlying pathogenesis of pulmonary hypertension is poorly understood. We hypothesized that microRNA-200b (miR-200b) plays a role in vascular remodeling associated with pulmonary hypertension. To better understand the role of miR-200b we created miR-200b knockout (KO) mice and aimed to 1) evaluate the morphological pulmonary vasculature changes in miR-200b KO mice and 2) to determine the role of miR-200b in pulmonary hypertension by targeting of the vascular endothelial growth factor (VEGF) signaling pathway. Method: Verhoeff-van Gieson (VVG) staining was used to measure the medial, arterial and adventitial wall thickness of the lung vessels. VEGF-A and its receptors VEGFR-1 and VEGFR-2 expression were assessed using immunohistochemistry (IHC). Micro-computed tomography (micro-CT) was optimized and applied to demonstrate the complexity of the pulmonary vasculature at the microlevel (8 µm) with high resolution, quantitative, three -dimensional images. Results: Vascular remodeling assessment showed that miR-200b KO lungs have 35% increased arterial wall thickness, 47% medial wall thickness and 32% adventitial wall thickness in pulmonary vessels compared to normal lung (P < 0.0001). The most significant structural changes were observed in arterioles with an external diameter less than 20 or 40 µm in the miR-200b KO group. IHC results showed that VEGF-A was downregulated and VEGFR-1 was upregulated in miR-200b KO lungs. However, VEGFR-2 expression did not show any difference between miR-200b KO and WT mice. Micro-CT data did not show any difference between miR-200b KO and WT mice. Conclusion: Pulmonary hypertension in miR-200b KO mice is associated with changes in vascular morphology. Our results suggest that the absence of miR-200b results in pulmonary hypertension by vascular remodeling of the pulmonary vessels especially in arterioles. MiR-200b could also contribute to pulmonary hypertension by downregulation of VEGF-A and upregulation of VEGFR-1. Advisors/Committee Members: Keijzer, Richard (Physiology and Pathophysiology) (supervisor), Czubryt, Michael (Physiology and Pathophysiology) Triggs-Raine, Barbara (Biochemistry and Medical Genetics) (examiningcommittee).

Subjects/Keywords: Congenital diaphragmatic hernia; pulmonary hypertension; miR-200b; knockout mice

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APA (6^th Edition):

Seif, S. (2019). Characterization of vascular remodeling associated with pulmonary hypertension in miR-200b knockout mice. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/34446

Chicago Manual of Style (16^th Edition):

Seif, Samira. “Characterization of vascular remodeling associated with pulmonary hypertension in miR-200b knockout mice.” 2019. Masters Thesis, University of Manitoba. Accessed January 18, 2021. http://hdl.handle.net/1993/34446.

MLA Handbook (7^th Edition):

Seif, Samira. “Characterization of vascular remodeling associated with pulmonary hypertension in miR-200b knockout mice.” 2019. Web. 18 Jan 2021.

Vancouver:

Seif S. Characterization of vascular remodeling associated with pulmonary hypertension in miR-200b knockout mice. [Internet] [Masters thesis]. University of Manitoba; 2019. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1993/34446.

Council of Science Editors:

Seif S. Characterization of vascular remodeling associated with pulmonary hypertension in miR-200b knockout mice. [Masters Thesis]. University of Manitoba; 2019. Available from: http://hdl.handle.net/1993/34446

University of Sydney

10. Chan, Ronald Ho Yeung. The Translocator Protein in Stress and Anxiety .

Degree: 2014, University of Sydney

URL: http://hdl.handle.net/2123/11704

► The translocator protein, or TSPO, is an evolutionarily conserved protein with many purported functions including cholesterol transport. Recently, its role in stress and anxiety disorders… (more)

Subjects/Keywords: Translocator protein; TSPO; TSPO knockout mice; anxiety; cannabinoids

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APA (6^th Edition):

Chan, R. H. Y. (2014). The Translocator Protein in Stress and Anxiety . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/11704

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chan, Ronald Ho Yeung. “The Translocator Protein in Stress and Anxiety .” 2014. Thesis, University of Sydney. Accessed January 18, 2021. http://hdl.handle.net/2123/11704.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chan, Ronald Ho Yeung. “The Translocator Protein in Stress and Anxiety .” 2014. Web. 18 Jan 2021.

Vancouver:

Chan RHY. The Translocator Protein in Stress and Anxiety . [Internet] [Thesis]. University of Sydney; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2123/11704.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chan RHY. The Translocator Protein in Stress and Anxiety . [Thesis]. University of Sydney; 2014. Available from: http://hdl.handle.net/2123/11704

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

11. Hook, Jeff. Functional identity of the Mammalian Gamma Tropomyosin Gene.

Degree: Medical Sciences, 2012, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52274 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10946/SOURCE01?view=true

► The actin filament system is fundamental to cellular functions including regulation of shape, motility, cytokinesis, intracellular trafficking and tissue organisation. Tropomyosins (Tm) are highly conserved… (more)

▼ The actin filament system is fundamental to cellular functions including regulation of shape, motility, cytokinesis, intracellular trafficking and tissue organisation. Tropomyosins (Tm) are highly conserved components of actin filaments which differentially regulate filament stability and function. The mammalian Tm family consists of four genes; alphaTm, betaTm, gammaTm and deltaTm (Tpm1-4). Multiple Tm isoforms (>40) are generated by alternative splicing, and the expression of these isoforms is highly regulated with spatial and temporal sorting of Tm isoforms into different cellular compartments. The importance of gammaTm gene products during mammalian development has not been fully explored. In order to further identify the role of mammalian Tm isoforms, the functional specificity of subsets of gammaTm gene family products in mice was tested using a series of gene knockouts. Knockout constructs of the amino-terminal exon 1b and carboxy-terminal exons 9a+9b, exon 9c and exon 9d from the gammaTm gene were used to assess the viability of ES cells and mice. The deletion of amino-terminal coding exon 1b ablates all eleven known gammaTm gene cytoskeletal products (Tm5NM1-11) and results in non-viable mice. However, the elimination of four exon 9c-containing isoforms (Tm5NM4,7,8,9) shows no impact on embryo viability whereas the deletion of two exon 9d-containing isoforms (Tm5NM1,2) results in partial lethality. The viability of knockout ES cells in vitro was also compromised as neither exon 1b nor exon 9d homozygous knockout ES cells could be generated. In contrast, homozygous knockout ES cells for exons 9a+9b (Tm5NM3,5,6,8,9,10,11) were viable. Results indicate that exons 9a+9b may be required for ES cells to undergo differentiation and a conditional knockout mouse model of exons 9a+9b is being generated to determine whether these gene products are required for embryogenesis. Furthermore, sperm lacking cytoskeletal Tm products of the gammaTm gene show preferential transmission of the deleted allele which may indicate a selective advantage. Since all four Tm genes are expressed in early embryos, ES cells and sperm, it is concluded that isoforms from the gammaTm gene perform specific functions in ES cell viability and embryogenesis that cannot be compensated by the other Tm genes. Advisors/Committee Members: Gunning, Peter, Medical Sciences, Faculty of Medicine, UNSW, Kavallaris, Maria, Children's Cancer Institute Australia for Medical Research, Faculty of Medicine, UNSW.

Subjects/Keywords: Knockout mice; Cytoskeleton; Tropomyosin; Gene targeting; Embryo development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hook, J. (2012). Functional identity of the Mammalian Gamma Tropomyosin Gene. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52274 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10946/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Hook, Jeff. “Functional identity of the Mammalian Gamma Tropomyosin Gene.” 2012. Doctoral Dissertation, University of New South Wales. Accessed January 18, 2021. http://handle.unsw.edu.au/1959.4/52274 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10946/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Hook, Jeff. “Functional identity of the Mammalian Gamma Tropomyosin Gene.” 2012. Web. 18 Jan 2021.

Vancouver:

Hook J. Functional identity of the Mammalian Gamma Tropomyosin Gene. [Internet] [Doctoral dissertation]. University of New South Wales; 2012. [cited 2021 Jan 18]. Available from: http://handle.unsw.edu.au/1959.4/52274 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10946/SOURCE01?view=true.

Council of Science Editors:

Hook J. Functional identity of the Mammalian Gamma Tropomyosin Gene. [Doctoral Dissertation]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/52274 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10946/SOURCE01?view=true

University of New South Wales

12. Shim, Doris Siew Chen. Use of knockout mice to investigate immunological roles of GM-CSF and GPR18.

Degree: Garvan Institute of Medical Research, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52931 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11609/SOURCE01?view=true

► The use of genetically modified mice has long been used as a method for understandingthe function of a gene of interest. In this thesis, we… (more)

▼ The use of genetically modified mice has long been used as a method for understandingthe function of a gene of interest. In this thesis, we aimed to examine the immunologicalroles of two genes in particular, GM-CSF (Section A) and GPR18 (Section B) throughthe use of in vivo models and genetically modified mice.GM-CSF is a cytokine with the capacity to promote inflammation in allergic airwayinflammation, which is driven by Th2 cells. Because of the importance of Th2 cells inparasite infections, the role of GM-CSF in mice infected with the nematodeNippostrongylus brasiliensis was examined using mice lacking functional GM-CSF(ΔGM-CSF mice), and mice lacking the cytokine receptor common β chain (Δβc mice),the latter being unable to signal in response to GM-CSF and IL-5.ΔGM-CSF mice showed no significant defect in parasite immunity in either primary orsecondary infection. By contrast, Δβc mice showed increased parasite burden, withhigher numbers of lung larvae after secondary infection and higher numbers ofintestinal worms and faecal eggs after both primary and secondary infection. There wereincreased numbers of circulating eosinophils in the ΔGM-CSF mice, associated withsignificantly reduced larval numbers in the lungs. These results indicate that GM-CSF isredundant in protection against N. brasiliensis infection, and that the increasedsusceptibility of Δβc mice to infection is likely to be attributed to the lack of IL-5signalling in these mice. The results suggest that clinical use of agents that neutraliseGM-CSF may not be associated with increased risk of parasite infection.GPR18 is a member of G-protein coupled receptors, a family of seven-transmembranereceptors shown to demonstrate a variety of biological functions. The ligand andphysiological functions of this orphan receptor remain unknown. Previous studies haveshown that GPR18 is highly expressed in lymphoid tissues. Mice lacking GPR18 in allcells (ΔGPR18 mice), in T cells (GPR18ΔT mice) and B cells (GPR18ΔB mice) weregenerated.viResults from this thesis did not detect a role for GPR18 in the normal functioning of Band T cells as shown by phenotyping studies in naïve and immunised GPR18ΔB andGPR18ΔT mice. On the other hand, data from the autoimmune model of experimentalautoimmune encephalomyelitis (EAE) model and Klebsiella pneumoniae infectionmodel have shown that GPR18 has a role in the regulation of these immune responses.ΔGPR18 mice were more likely to display symptoms of atypical EAE compared to WTmice, and this was associated with increased IL-17 production. During theK.pneumoniae infection, ΔGPR18 mice had decreased bacterial burden compared tocontrol mice, which again was associated with increased IL-17 production. Theseexperiments indicate that GPR18 has a role in downregulating IL-17 in immuneresponses. Advisors/Committee Members: Sewell, William, Clinical School - St Vincent's Hospital, Faculty of Medicine, UNSW.

Subjects/Keywords: Inflammation; GM-CSF; GPR18; Knockout mice; Immunological function

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shim, D. S. C. (2013). Use of knockout mice to investigate immunological roles of GM-CSF and GPR18. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52931 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11609/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Shim, Doris Siew Chen. “Use of knockout mice to investigate immunological roles of GM-CSF and GPR18.” 2013. Doctoral Dissertation, University of New South Wales. Accessed January 18, 2021. http://handle.unsw.edu.au/1959.4/52931 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11609/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Shim, Doris Siew Chen. “Use of knockout mice to investigate immunological roles of GM-CSF and GPR18.” 2013. Web. 18 Jan 2021.

Vancouver:

Shim DSC. Use of knockout mice to investigate immunological roles of GM-CSF and GPR18. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Jan 18]. Available from: http://handle.unsw.edu.au/1959.4/52931 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11609/SOURCE01?view=true.

Council of Science Editors:

Shim DSC. Use of knockout mice to investigate immunological roles of GM-CSF and GPR18. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/52931 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11609/SOURCE01?view=true

13. Oliveira, Katharina Morant Holanda de. Ausência da interleucina-22 interfere na microbiota bucal e na progressão de lesões periapicais induzidas em dentes de camundongos.

Degree: Mestrado, Odontopediatria, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/58/58135/tde-17052013-164100/ ;

► Introdução: O objetivo deste trabalho foi caracterizar a composição da microbiota bucal e a formação e progressão de lesões periapicais induzidas experimentalmente em dentes de… (more)

▼ Introdução: O objetivo deste trabalho foi caracterizar a composição da microbiota bucal e a formação e progressão de lesões periapicais induzidas experimentalmente em dentes de camundongos knockout para IL-22 (IL-22 KO) comparados com animais wild-type (WT). Material e Métodos: Inicialmente, foi realizada a avaliação do perfil microbiano da cavidade bucal dos animais (40 espécies de micro-organismos), no dia das exposições pulpares, por meio de técnica de biologia molecular (Checkerboard DNA-DNA hybridization). Em seguida, lesões periapicais foram induzidas nos primeiros molares inferiores dos camundongos e, decorridos os períodos de 7, 21 e 42 dias, os animais foram submetidos à eutanásia em câmara de CO2. As mandíbulas foram então removidas e submetidas ao processamento histotécnico. A seguir, cortes representativos de cada dente foram corados com hematoxilina e eosina (HE), para descrição do tecido pulpar e das regiões apical e periapical, em microscopia óptica convencional e mensuração da área das lesões periapicais em microscopia de fluorescência. Além disso, cortes sequenciais foram avaliados por meio de: histoenzimologia para a marcação de osteoclastos (TRAP), coloração de Brown & Brenn (para identificação de bactérias) e imunohistoquímica (para identificação de RANK, RANKL e OPG). Os escores da quantidade de células bacterianas, para cada uma das 40 espécies avaliadas, foram submetidos à analise estatística empregando o teste não-paramétrico de Mann-Whitney para amostras independentes, para comparação entre os grupos. Os resultados numéricos obtidos na análise morfométrica da área das lesões periapicais e do número de osteoclastos foram submetidos à análise estatística öne-wayÄNOVA e pós-teste de Bonferroni. Para todas as análises foi adotado o nível de significância de 5%. Resultados: Em relação ao perfil microbiano encontrado na cavidade bucal dos animais, foi possível observar diferença estatisticamente siginificante entre os dois grupos de animais para 6 espécies bacterianas (p<0,05), sendo 5 dessas espécies mais encontradas nos animais WT e apenas 1 encontrada em maior quantidade nos animais IL-22 KO. Já em relação à análise microscópica, o grupo dos animais WT mostrou diferença estatisticamente significante entre 7 e 42 dias e entre 21 e 42 dias, com aumento progressivo no tamanho das lesões e no número de osteoclastos (p<0,05). No grupo dos animais IL-22 KO, houve um aumento do tamanho da lesão e do número de osteoclastos entre 7 e 21 dias, seguido de diminuição desses parâmetros entre 21 e 42 dias, com diferença significante (p<0,05) entre 7 e 21 dias. Além disso, na comparação entre os dois tipos de animais, foram encontradas diferenças significantes (p<0,05) em relação ao tamanho das lesões periapicais e ao número de osteoclastos aos 42 dias, sem diferenças em relação à localização de bactérias e imunohistoquímica. Conclusões: Esse estudo demonstrou diferenças na composição da microbiota bucal dos animais WT e IL-22 KO, o que pode ter influenciado na formação das lesões periapicais. Além disso,… Advisors/Committee Members: Segato, Raquel Assed Bezerra.

Subjects/Keywords: camundongos knockout; immunohistochemistry; imunohistoquímica; inflamação; inflammation; Interleucina-22; Interleukin-22; knockout mice; lesão periapical; micro-organismos; microorganisms; osteoclastos; osteoclasts; periapical lesion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, K. M. H. d. (2013). Ausência da interleucina-22 interfere na microbiota bucal e na progressão de lesões periapicais induzidas em dentes de camundongos. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/58/58135/tde-17052013-164100/ ;

Chicago Manual of Style (16^th Edition):

Oliveira, Katharina Morant Holanda de. “Ausência da interleucina-22 interfere na microbiota bucal e na progressão de lesões periapicais induzidas em dentes de camundongos.” 2013. Masters Thesis, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/58/58135/tde-17052013-164100/ ;.

MLA Handbook (7^th Edition):

Oliveira, Katharina Morant Holanda de. “Ausência da interleucina-22 interfere na microbiota bucal e na progressão de lesões periapicais induzidas em dentes de camundongos.” 2013. Web. 18 Jan 2021.

Vancouver:

Oliveira KMHd. Ausência da interleucina-22 interfere na microbiota bucal e na progressão de lesões periapicais induzidas em dentes de camundongos. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/58/58135/tde-17052013-164100/ ;.

Council of Science Editors:

Oliveira KMHd. Ausência da interleucina-22 interfere na microbiota bucal e na progressão de lesões periapicais induzidas em dentes de camundongos. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/58/58135/tde-17052013-164100/ ;

14. Uran Jimenez, Martha Eugenia. Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade.

Degree: PhD, Dermatologia, 2015, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5133/tde-04082015-090755/ ;

► A paracoccidioidomicose (PCM) é uma doença granulomatosa sistêmica, causada por Paracoccidioides spp., (P. brasiliensis e P. lutzii), geograficamente, limita-se a América Latina com as áreas… (more)

▼ A paracoccidioidomicose (PCM) é uma doença granulomatosa sistêmica, causada por Paracoccidioides spp., (P. brasiliensis e P. lutzii), geograficamente, limita-se a América Latina com as áreas endêmicas estendendo-se desde o México até a Argentina, constituindo uma das micoses sistêmicas de maior incidência na região, afetando principalmente trabalhadores rurais. O maior número de pacientes com PCM tem sido reportado principalmente no Brasil, Colômbia e Venezuela. A incidência real desta micose encontra-se subestimada no Brasil e pouco se conhece em relação a nova espécie descrita - P. lutzii. A maioria dos estudos em P. lutzii foram focados em genética, especiação e na geração de novos antígenos para melhorar a especificidade e sensibilidade dos testes sorológicos. Atualmente, as preparações antigênicas tradicionais, preparadas a partir de isolados de P. brasiliensis, são ineficientes. Raros são os trabalhos focados na biologia de P. lutzii e nos fatores de virulência que podem ser comparados com P. brasiliensis nos modelos experimentais. A nossa proposta de estudo foi avaliar alguns aspectos in vitro e in vivo relacionados com a patogenicidade e destacamos: a fagocitose e a morte intracelular de P. lutzii por macrófagos, peritoneais, de camundongos Knockouts (KO) e selvagens para PRRs (TLR2, TLR4 e Dectina) e ativadores intracelulares (MyD88 e NALP3). Paralelamente a este estudo, animais foram infectados com leveduras de P. lutzii e comparados com os modelos de infecção já estabelecidos com leveduras (Pb18) e conídios (ATCCPb60855) de P. brasiliensis. Nossos dados indicam que similar ao que ocorre com P. brasiliensis a fagocitose de P. lutzii depende de TLR2, TLR4 e Dectina- 1, resultados semelhantes também foram observadas na expressão de moléculas envolvidas na co-estimulação e a apresentação de antígenos (MHC II, CD80 e CD86). Contudo, a morte intracelular de leveduras de P. lutzii é claramente dependente de TLR4, e a produção de citocinas IL-6, MIP-2, IFN- e IL-12p40 são importantes para o controle das leveduras pelos macrófagos. No modelo experimental de P. lutzii, camundongos machos C57BL/6 (6-7 semanas) foram infectados intratraquealmente como 1x106 leveduras viáveis do isolado de P. lutzii Pb01. Encontramos duas fases da doença, a primeira de 0 hora até 2 a 4 semanas pós-infecção, e a segunda de 4 até 12 semanas. As leveduras parecem ser contidas na primeira semana de infecção e posteriormente não encontramos leveduras nos macerados de pulmão, diferente do modelo de BALB/c infetado com conídios de ATCC-Pb60855 no qual as UFC são recuperadas até a semana 16 pós-infeção. Como relação aos níveis de citocinas, encontramos que na lavagem broncoalveolar e macerado de pulmão um perfil misto Th1/Th2 porém, marcado por citocinas próinflamatórias no primeiro período e citocinas regulatórias tipo Th2 no segundo período (IL-12p70, IL-23, IL-10); similar ao descrito nos modelos de P. brasiliensis infectados tanto com conídios como com leveduras. No entanto, no primeiro período da doença, em camundongos C57BL/6, parece ter… Advisors/Committee Members: Taborda, Carlos Pelleschi.

Subjects/Keywords: Camundongos Knockout; Conidia; Conídios; Leveduras; Mice Knockout; Micélio; Micologia; Mycelium; Mycology; Paracoccidioides/immunology; Paracoccidioides/imunologia; Paracoccidioidomicose; Paracoccidioidomycosis; Yeats

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Uran Jimenez, M. E. (2015). Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5133/tde-04082015-090755/ ;

Chicago Manual of Style (16^th Edition):

Uran Jimenez, Martha Eugenia. “Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade.” 2015. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/5/5133/tde-04082015-090755/ ;.

MLA Handbook (7^th Edition):

Uran Jimenez, Martha Eugenia. “Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade.” 2015. Web. 18 Jan 2021.

Vancouver:

Uran Jimenez ME. Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade. [Internet] [Doctoral dissertation]. University of São Paulo; 2015. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5133/tde-04082015-090755/ ;.

Council of Science Editors:

Uran Jimenez ME. Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade. [Doctoral Dissertation]. University of São Paulo; 2015. Available from: http://www.teses.usp.br/teses/disponiveis/5/5133/tde-04082015-090755/ ;

15. Cardinot, Themis Moura. Efeito do treinamento físico aeróbico na prevenção e terapêutica da doença aterosclerótica em modelo experimental de aterosclerose.

Degree: PhD, Emergências Clínicas, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5159/tde-25062009-114558/ ;

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O conhecimento de que o exercício é benéfico na doença aterosclerótica é baseado principalmente em estudos epidemiológicos. O objetivo deste trabalho foi investigar se o… (more)

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O conhecimento de que o exercício é benéfico na doença aterosclerótica é baseado principalmente em estudos epidemiológicos. O objetivo deste trabalho foi investigar se o treinamento físico preventivo ou terapêutico altera a evolução da placa aterosclerótica. Camundongos LDLr-/- com 16 semanas de vida foram separados em dois programas: preventivo e terapêutico. Animais do programa preventivo receberam dieta normal ou aterogênica por 14 semanas. O treinamento físico foi iniciado concomitantemente ao início da dieta. Animais do programa terapêutico receberam dieta normal ou aterogênica por 28 semanas. O treinamento físico foi iniciado após 14 semanas do início da dieta, com placas bem estabelecidas. O treinamento físico aeróbico moderado foi realizado em esteira rolante, por 60 min, 5 dias/sem, durante 14 semanas. Massa corporal, pressão arterial caudal e freqüência cardíaca foram registradas. Lipoproteínas plasmáticas foram separadas por FPLC e colesterol total foi dosado por métodos enzimáticos. Foram quantificados tamanho, conteúdo de gordura e de colágeno da placa por coloração de oil-red O e picro-sirius. Citocinas TNF-, IL-6 foram medidas por Elisa. MMP-9 plasmática foi medida por zimografia. Marcadores inflamatórios teciduais, MMP-9, CD40/CD40L e nitrotirosina, foram medidos na placa por imunohistoquímica. O treinamento físico não modificou o tamanho da placa, mas tornou a placa mais estável por aumentar o conteúdo de colágeno. O treinamento físico diminuiu o conteúdo de gordura da placa, os fatores de risco e o CD40 somente no programa preventivo. Nenhuma alteração foi notada nos marcadores inflamatórios circulantes e na expressão de MMP-9 e formação de nitrotirosina na placa aterosclerótica.

The knowledge that exercise exerts beneficial effects on atherosclerotic disease is mainly based on epidemiological studies. Our aim was to investigate the effect of preventive and therapeutic exercise programs on atherosclerotic plaque formation and development. Sixteen-week-old LDLr-/- mice were randomly divided into preventive and therapeutic programs. Preventive programs mice received normal or atherogenic diet for 14 weeks. Exercise training started at the same time of dieting. Therapeutic programs mice received normal or atherogenic diet for 28 weeks. Exercise training started after 14 weeks of dieting when atherosclerosis plaques were already established. Moderate intensity aerobic exercise training was performed on a motor treadmill for 60 min, 5 days/wk, during 14 weeks. Body mass, caudal blood pressure and heart rate were registered. Plasma lipoproteins were separated by FPLC and total cholesterol was determined by enzymatic methods. Cross sections of aortic root were stained with oil-red O for plaque size and fat content. Aorta longitudinal sections were stained with picro-sirius for collagen content. TNF- and IL-6 cytokines were measured by Elisa. Plasmatic MMP-9 was determined by zimography. Inflammatory tissue markers, MMP-9, CD40/CD40L and nitrotirosine, were measured by immunohistochemistry. We concluded…

Advisors/Committee Members: Souza, Heraldo Possolo de.

Subjects/Keywords: Aterosclerose/prevenção & controle; Aterosclerose/terapia; Atherosclerosis/prevention & control; Atherosclerosis/therapy; Camundongos Knockout; Exercício; Exercise; Mice Knockout

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cardinot, T. M. (2009). Efeito do treinamento físico aeróbico na prevenção e terapêutica da doença aterosclerótica em modelo experimental de aterosclerose. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5159/tde-25062009-114558/ ;

Chicago Manual of Style (16^th Edition):

Cardinot, Themis Moura. “Efeito do treinamento físico aeróbico na prevenção e terapêutica da doença aterosclerótica em modelo experimental de aterosclerose.” 2009. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/5/5159/tde-25062009-114558/ ;.

MLA Handbook (7^th Edition):

Cardinot, Themis Moura. “Efeito do treinamento físico aeróbico na prevenção e terapêutica da doença aterosclerótica em modelo experimental de aterosclerose.” 2009. Web. 18 Jan 2021.

Vancouver:

Cardinot TM. Efeito do treinamento físico aeróbico na prevenção e terapêutica da doença aterosclerótica em modelo experimental de aterosclerose. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5159/tde-25062009-114558/ ;.

Council of Science Editors:

Cardinot TM. Efeito do treinamento físico aeróbico na prevenção e terapêutica da doença aterosclerótica em modelo experimental de aterosclerose. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/5/5159/tde-25062009-114558/ ;

16. Lucisano, Marília Pacifico. Avaliação de lesões periapicais induzidas experimentalmente em camundongos knockout para molécula adaptadora para ativação de receptores Toll-like (MyD88).

Degree: PhD, Odontopediatria, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/58/58135/tde-03042013-164033/ ;

► A molécula adaptadora myeloid differentiation primary response gene 88 (MyD88) está envolvida na ativação de receptores Toll-like (TLRs), os quais são responsáveis pelo reconhecimento precoce… (more)

▼ A molécula adaptadora myeloid differentiation primary response gene 88 (MyD88) está envolvida na ativação de receptores Toll-like (TLRs), os quais são responsáveis pelo reconhecimento precoce pelas células do hospedeiro de patógenos invasores e pelo desencadeamento da resposta imunológica. O objetivo do presente estudo foi caracterizar o desenvolvimento e a progressão de lesões periapicais induzidas experimentalmente em dentes de camundongos knockout (KO) para a molécula MyD88 (MyD88 KO), comparados a animais wild-type (WT). Lesões periapicais foram induzidas nos primeiros molares inferiores de 30 camundongos WT e de 30 camundongos MyD88 KO. Decorridos 7, 21 e 42 dias, os animais foram submetidos à eutanásia em câmara de CO2 e as mandíbulas foram removidas e submetidas ao processamento histotécnico. A seguir, cortes representativos foram corados com hematoxilina e eosina (HE), para descrição das características do canal radicular e das regiões apical e periapical e para contagem do número de células inflamatórias (neutrófilos), em microscopia de luz, e para mensuração da área das lesões periapicais, em microscopia de fluorescência. Espécimes sequenciais foram analisados por meio de: histoenzimologia para a atividade da TRAP, para contagem de osteoclastos; coloração de Brown & Brenn, para localização de bactérias; e imunohistoquímica, para identificação de marcadores da osteoclastogênese (RANK, RANKL e OPG). Os dados foram submetidos à análise estatística por meio dos testes não-paramétricos de Mann-Whitney, Kruskal-Wallis e pós-teste de Dunn, utilizando o programa SPSS (Statistical Package for the Social Sciences) versão 17.O, com nível de significância de 5%. As demais análises foram expressas de maneira qualitativa. Com relação à extensão das lesões periapicais, o grupo MyD88 KO apresentou valores significantemente maiores do que o grupo WT nos períodos de 7 (p=0,001) e 21 dias (p=0,05), sendo que após 42 dias foi observada tendência de maiores valores, porém sem diferença significante (p=0,09). Foi observada maior quantidade de neutrófilos no grupo MyD88 KO, em comparação aos animais WT (p=0,01 em 7 dias; p=0,004 em 21 dias; e p<0,001 em 42 dias). Por outro lado, com relação à quantidade de osteoclastos, não foi observada diferença significante entre ambos os grupos, em todos os períodos experimentais (p=0,884 em 7 dias; p=0,506 em 21 dias; e p=0,211 em 42 dias). A análise microscópica descritiva do grupo MyD88 KO revelou um infiltrado inflamatório mais intenso, com presença abundante de células porlimorfonucleadas e mononucleadas e com grande destruição tecidual, após 7, 21 e 42 dias. A coloração de Brown e Brenn evidenciou uma maior disseminação bacteriana, inclusive nos tecidos periapicais, no grupo MyD88 KO, quando comparado aos animais WT. Com relação à imunohistoquímica, foram observadas marcações para RANK, RANKL e OPG de forma semelhante entre os dois grupos de animais. Com base nas metodologias e nos resultados obtidos no presente estudo pode-se concluir que na ausência da MyD88 os animais apresentaram… Advisors/Committee Members: Nelson Filho, Paulo, Segato, Raquel Assed Bezerra.

Subjects/Keywords: knockout mice; toll-like receptors; camundongos knockout; lesão periapical; molécula MyD88; MyD88 molecule; periapical lesion; receptores toll-like

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lucisano, M. P. (2013). Avaliação de lesões periapicais induzidas experimentalmente em camundongos knockout para molécula adaptadora para ativação de receptores Toll-like (MyD88). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/58/58135/tde-03042013-164033/ ;

Chicago Manual of Style (16^th Edition):

Lucisano, Marília Pacifico. “Avaliação de lesões periapicais induzidas experimentalmente em camundongos knockout para molécula adaptadora para ativação de receptores Toll-like (MyD88).” 2013. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/58/58135/tde-03042013-164033/ ;.

MLA Handbook (7^th Edition):

Lucisano, Marília Pacifico. “Avaliação de lesões periapicais induzidas experimentalmente em camundongos knockout para molécula adaptadora para ativação de receptores Toll-like (MyD88).” 2013. Web. 18 Jan 2021.

Vancouver:

Lucisano MP. Avaliação de lesões periapicais induzidas experimentalmente em camundongos knockout para molécula adaptadora para ativação de receptores Toll-like (MyD88). [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/58/58135/tde-03042013-164033/ ;.

Council of Science Editors:

Lucisano MP. Avaliação de lesões periapicais induzidas experimentalmente em camundongos knockout para molécula adaptadora para ativação de receptores Toll-like (MyD88). [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/58/58135/tde-03042013-164033/ ;

Université Montpellier II

17. Birchenall, Alix. Rôle du facteur de transcription Meis2 dans les dérivés de la crête neurale par l'étude des souris Wnt1crecKOMeis2-/- et Islet1cre/+cKOMeis2-/- : Role of the transcription factor Meis2 in neural crest derivatives using Wnt1crecKOMeis2-/- and Islet1cre/+ cKOMeis2-/- strains.

Degree: Docteur es, Neurobiologie des processus de communication et d'intégration, 2012, Université Montpellier II

URL: http://www.theses.fr/2012MON20216

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Le système nerveux somatosensoriel permet l'interaction entre l'organisme et son environnement. Ce système collecte, via des récepteurs périphériques, les stimuli extérieurs et les transmet au… (more)

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Le système nerveux somatosensoriel permet l'interaction entre l'organisme et son environnement. Ce système collecte, via des récepteurs périphériques, les stimuli extérieurs et les transmet au système nerveux central par les neurones sensoriels primaires, dont les corps cellulaires sont situés dans les ganglions rachidiens dorsaux. Ces neurones primaires sont spécifiques des différentes sensations et ont, pour y répondre, des récepteurs, des modalités sensorielles, des caractéristiques moléculaires différentes. Ils sont généralement séparés en 3 grandes familles: les propriocepteurs, les mécanocepteurs et les nocicepteurs, chacune de ces familles se séparant à son tour en une multitude de sous familles. Ces neurones dérivent de la crête neurale, une structure spécifique des vertébrés. Au cours de leur migration vers les ganglions rachidiens dorsaux, les cellules vont être soumises à un grand nombre de facteurs et de voies de signalisation, qui vont entrainer leur survie, leur mort ou leur différenciation. Le facteur de transcription Meis2 a été isolé par l'équipe comme un candidat pouvant intervenir dans cette différentiation des cellules en neurones différenciés. Chez les souris, son expression est spécifique de sous populations mécanoceptives et proprioceptives, et s'étend des stades précoces de développement jusqu'à l'âge adulte. La lignée conditionnelle de souris Knock Out pour Meis2, croisée avec la lignée Wnt1cre, permet l'abolition de Meis2 dans toutes les cellules de la crête neurale et ses dérivés. Le mutant issu de ce croisement meurt à la naissance, avec de nombreux problèmes phénotypiques. Cette lignée cKOMeis2 a alors été croisée avec la lignée Islet1cre, ce qui permet d'invalider le gène Meis2 dans les neurones post-mitotiques des ganglions rachidiens dorsaux. Cette souris m'a servi de modèle afin de déterminer les conséquences éventuelles de la perte de Meis2 dans les neurones sensoriels du ganglion rachidien dorsal par analyse comportementale.

The somatosensory nervous system allows the interaction between the organism and the environment. This system receives from peripheral receptors some exterior stimuli which are transmitted to the central nervous system by sensory primary neurons. Their cell bodies are located in the dorsal root ganglions (DRG). These primary neurons are specific to various sensations and are characterized by specific receptors, sensory modalities and molecular characteristics involved in their response. They are usually defined as belonging to one of three main families: proprioceptors, mecanoceptors and nociceptors, and each family is composed of a large number of subgroups. These neurons are derived from the neural crest cells to form the DRG. The cells are exposed to a number of key pathways and factors, which permit their survival, death or differentiation. The transcription factor Meis2 was isolated by our team as a good candidate to act in the differentiation or specification of these cells into sensory neurons. The expression pattern of Meis2 is shown to be specific to…

Advisors/Committee Members: Marmigère, Frédéric (thesis director), Valmier, Jean (thesis director).

Subjects/Keywords: Mécanoréception; Proprioception; Souris knockout; Biologie sensorielle; Dérivés de la crête neurale; Mechanoreceptive; Proprioception; Knockout mice; Sensory biology; Neural crest derivatives

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Birchenall, A. (2012). Rôle du facteur de transcription Meis2 dans les dérivés de la crête neurale par l'étude des souris Wnt1crecKOMeis2-/- et Islet1cre/+cKOMeis2-/- : Role of the transcription factor Meis2 in neural crest derivatives using Wnt1crecKOMeis2-/- and Islet1cre/+ cKOMeis2-/- strains. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2012MON20216

Chicago Manual of Style (16^th Edition):

Birchenall, Alix. “Rôle du facteur de transcription Meis2 dans les dérivés de la crête neurale par l'étude des souris Wnt1crecKOMeis2-/- et Islet1cre/+cKOMeis2-/- : Role of the transcription factor Meis2 in neural crest derivatives using Wnt1crecKOMeis2-/- and Islet1cre/+ cKOMeis2-/- strains.” 2012. Doctoral Dissertation, Université Montpellier II. Accessed January 18, 2021. http://www.theses.fr/2012MON20216.

MLA Handbook (7^th Edition):

Birchenall, Alix. “Rôle du facteur de transcription Meis2 dans les dérivés de la crête neurale par l'étude des souris Wnt1crecKOMeis2-/- et Islet1cre/+cKOMeis2-/- : Role of the transcription factor Meis2 in neural crest derivatives using Wnt1crecKOMeis2-/- and Islet1cre/+ cKOMeis2-/- strains.” 2012. Web. 18 Jan 2021.

Vancouver:

Birchenall A. Rôle du facteur de transcription Meis2 dans les dérivés de la crête neurale par l'étude des souris Wnt1crecKOMeis2-/- et Islet1cre/+cKOMeis2-/- : Role of the transcription factor Meis2 in neural crest derivatives using Wnt1crecKOMeis2-/- and Islet1cre/+ cKOMeis2-/- strains. [Internet] [Doctoral dissertation]. Université Montpellier II; 2012. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2012MON20216.

Council of Science Editors:

Birchenall A. Rôle du facteur de transcription Meis2 dans les dérivés de la crête neurale par l'étude des souris Wnt1crecKOMeis2-/- et Islet1cre/+cKOMeis2-/- : Role of the transcription factor Meis2 in neural crest derivatives using Wnt1crecKOMeis2-/- and Islet1cre/+ cKOMeis2-/- strains. [Doctoral Dissertation]. Université Montpellier II; 2012. Available from: http://www.theses.fr/2012MON20216

Universidade do Estado do Rio de Janeiro

18. Sebastião Barreto de Brito Filho. Efeito da ingestão crônica de vinho sobre a homeostase glicêmica, lipídica e ponderal em camundongos ApoE Knockout.

Degree: PhD, 2013, Universidade do Estado do Rio de Janeiro

URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=6770 ;

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Os benefícios à saúde relacionados ao consumo moderado de vinho incluem diferentes mecanismos, nos quais estão envolvidos tanto etanol quanto compostos fenólicos que são constituintes… (more)

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Os benefícios à saúde relacionados ao consumo moderado de vinho incluem diferentes mecanismos, nos quais estão envolvidos tanto etanol quanto compostos fenólicos que são constituintes do mesmo. Com o objetivo de avaliar variações glicêmicas, ponderais e o depósito de triglicérides, colesterol e glicogênio hepáticos com uso regular de vinho tinto em camundongo ApoE Knockout, foram utilizados 60 camundongos machos adultos ApoE Knockout de peso médio de 30 gramas, distribuídos em três grupos de 20 animais: grupo vinho, grupo etanol e grupo água, os quais receberam 50 mL de vinho e 50 mL água, 6mL de etanol e 94mL de água e somente água respectivamente por quatro meses. Os parâmetros avaliados foram: variações glicêmicas, ponderais, acúmulo de triglicerídeos, colesterol e glicogênio hepáticos. O grupo vinho teve em relação a sua massa corporal uma área sob a curva maior que a dos outros dois grupos, mas com um percentual pequeno de aumento. A concentração do triglicerídeo hepático foi maior no grupo vinho 57% em relação ao grupo etanol, que foi 31,6% menor que o controle (p<0,01%). A concentração do colesterol hepático foi menor no grupo vinho (23,6%), assim como no grupo etanol (24,5%), (p<0,05%). A concentração do glicogênio hepático foi maior no grupo vinho (16%), porém não alcançando significado estatístico. A glicemia em jejum no dia da eutanásia foi maior no grupo etanol em relação aos demais grupos, porém não demonstrou diferença estatisticamente significante. Na análise histológica não foi observada diferença significativa entre os grupos, embora o peso médio em gramas nas gorduras, retroperitoneal e subcutâneas tenha sido aproximadamente duas vezes maior no grupo vinho. Concluiu-se que neste estudo o uso regular e crônico de vinho tinto aumentou triglicerídeo hepático, porém o álcool diminui o colesterol hepático. O aumento do triglicerídeo pode ser devido ao alto valor calórico do vinho ou alguma propriedade lipogênica desconhecida que levou ao aumento importante das gorduras retroperitoneais e subcutâneas em camundongos ApoE Knockout.

The benefits to health related to regular consume of red wine includes different mechanisms in which are involved both ethanol and fenolics compounds of the wine. With the objective to evaluate glycemia, lipid profile and weight variations with regular use of red wine by ApoE Knockout mices, sixty adults ApoE Knockout mices weighing around 30g were distributed into 3 groups of 20 animals each: 1.Wine that received 50mL of wine plus 50mL of water, 2. Ethanol and Water groups, 6mL of ethanol plus 94mL of water and just water respectively for 4 months. We evaluate glycemia, weight variations and liver glycogen, triglycerides and cholesterol. The wine group had in relation to its mass body an area under the curve larger than the other two groups, but with a small percentage of increase. The concentration of liver triglycerides was higher in the wine 57% compared to ethanol group, which was 31.6% lower than the control (p<0.01%). The concentration of liver cholesterol was lower in…

Advisors/Committee Members: Egberto Gaspar de Moura, Mariana Sarto Figueiredo, Maria do Rosário da Silva Ramos Costa, Elias Amorim, Orlando José dos Santos, Viviane Younes Rapozo.

Subjects/Keywords: Vinho; Homeostase glicêmica e ponderal; Perfil lipídico; Camundongo ApoE Knockout; Wine; Lipidic profile; Glycemic and weight Homeothasis; ApoE Knockout mice; FISIOLOGIA ENDOCRINA; Vinho Teses; Homeostase Teses; Camundongos Knockout

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Filho, S. B. d. B. (2013). Efeito da ingestão crônica de vinho sobre a homeostase glicêmica, lipídica e ponderal em camundongos ApoE Knockout. (Doctoral Dissertation). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=6770 ;

Chicago Manual of Style (16^th Edition):

Filho, Sebastião Barreto de Brito. “Efeito da ingestão crônica de vinho sobre a homeostase glicêmica, lipídica e ponderal em camundongos ApoE Knockout.” 2013. Doctoral Dissertation, Universidade do Estado do Rio de Janeiro. Accessed January 18, 2021. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=6770 ;.

MLA Handbook (7^th Edition):

Filho, Sebastião Barreto de Brito. “Efeito da ingestão crônica de vinho sobre a homeostase glicêmica, lipídica e ponderal em camundongos ApoE Knockout.” 2013. Web. 18 Jan 2021.

Vancouver:

Filho SBdB. Efeito da ingestão crônica de vinho sobre a homeostase glicêmica, lipídica e ponderal em camundongos ApoE Knockout. [Internet] [Doctoral dissertation]. Universidade do Estado do Rio de Janeiro; 2013. [cited 2021 Jan 18]. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=6770 ;.

Council of Science Editors:

Filho SBdB. Efeito da ingestão crônica de vinho sobre a homeostase glicêmica, lipídica e ponderal em camundongos ApoE Knockout. [Doctoral Dissertation]. Universidade do Estado do Rio de Janeiro; 2013. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=6770 ;

19. Fouad, Gameil Taher. Investigating the biological role of Creld1 as a candidate gene for congenital heart defects.

Degree: PhD, 2002, Oregon Health Sciences University

URL: doi:10.6083/M41R6NS5 ; http://digitalcommons.ohsu.edu/etd/3173

Subjects/Keywords: Heart Defects, Congenital – genetics; Mice, Knockout

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fouad, G. T. (2002). Investigating the biological role of Creld1 as a candidate gene for congenital heart defects. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M41R6NS5 ; http://digitalcommons.ohsu.edu/etd/3173

Chicago Manual of Style (16^th Edition):

Fouad, Gameil Taher. “Investigating the biological role of Creld1 as a candidate gene for congenital heart defects.” 2002. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 18, 2021. doi:10.6083/M41R6NS5 ; http://digitalcommons.ohsu.edu/etd/3173.

MLA Handbook (7^th Edition):

Fouad, Gameil Taher. “Investigating the biological role of Creld1 as a candidate gene for congenital heart defects.” 2002. Web. 18 Jan 2021.

Vancouver:

Fouad GT. Investigating the biological role of Creld1 as a candidate gene for congenital heart defects. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 2002. [cited 2021 Jan 18]. Available from: doi:10.6083/M41R6NS5 ; http://digitalcommons.ohsu.edu/etd/3173.

Council of Science Editors:

Fouad GT. Investigating the biological role of Creld1 as a candidate gene for congenital heart defects. [Doctoral Dissertation]. Oregon Health Sciences University; 2002. Available from: doi:10.6083/M41R6NS5 ; http://digitalcommons.ohsu.edu/etd/3173

20. Sansig, Gilles. Des souris knock-out pour le récepteur métabotrope au glutamate mGluR7 révèlent son rôle dans la cognition et les émotions : Knockout mice for the metabotropic glutamate receptor mGluR7 reveal its role in cognition and emotions.

Degree: Docteur es, Neurosciences, 2016, Université de Strasbourg

URL: http://www.theses.fr/2016STRAJ018

► L’un des domaines clés de la recherche en neurosciences modernes consiste à comprendre les interactions complexes entre le stress et la génétique qui conduisent à… (more)

▼ L’un des domaines clés de la recherche en neurosciences modernes consiste à comprendre les interactions complexes entre le stress et la génétique qui conduisent à la manifestation de troubles tels que la dépression, l’anxiété et le dysfonctionnement cognitif. Des preuves de plus en plus nombreuses suggèrent que le système glutamatergique peut être une cible thérapeutique pertinente pour de tels troubles. Le glutamate est le neurotransmetteur utilisé par la grande majorité des synapses excitatrices dans le cerveau. Et les sous-types des récepteurs métabotropique au glutamate (mGluR1 – mGluR8) agissent avant tout comme d’importants régulateurs postsynaptiques de la neurotransmission dans le système nerveux central (SNC), en fournissant un mécanisme par lequel les réponses synaptiques rapides à travers des canaux cationiques dépendants du glutamate peuvent être affinées. Ainsi, les récepteurs mGluR participent à une grande variété de fonctions du système nerveux central. Au sein dela famille des récepteurs métabotropiques au glutamate, le récepteur présynaptique mGluR7 montre la conservation évolutive la plus élevée et on pense qu'il agit comme un régulateur de la libération de neurotransmetteurs. Le récepteur mGluR7 est également le plus largement distribué des récepteurs présynaptiques mGluR, présent sur une large gamme de synapses démontrées comme critiques à la fois dans le fonctionnement normal du système nerveux central, mais également dans une large gamme de troubles psychiatriques et neurologiques. De plus, un nombre croissant de preuves expérimentales suggèrent que le récepteur mGluR7 est non seulement un acteur clé dans l’élaboration de réponses synaptiques au niveau des synapses glutamatergiques, mais qu’il est également un régulateur clé de la transmission GABAergique inhibitrice. Le développement d’outils pharmacologiques et génétiques sélectifs a permis le démantèlement de la fonction du récepteur mGluR7 dans une multitude de processus physiologiques et comportementaux. Ainsi les souris knock-out ont mis en évidence un rôle du récepteur mGluR7 dans l’anxiété, le conditionnement de la peur, l’aversion, l’apprentissage et la mémoire spatiale. De plus, ces souris dépourvues du récepteur métabrotrope mGluR7 démontrent une sensibilité accrue aux crises épileptiques suggérant un rôle unique de ce récepteur dans la régulation de l’excitabilité neuronale. De même, une altération de la plasticité synaptique à court terme dans les souris transgéniques dépourvues du récepteur métabotrope au glutamate mGluR7 démontre que l’absence de récepteurs mGluR7 engendre des altérations de la plasticité synaptique à court terme dans l’hippocampe. En outre, la découverte et la caractérisation récente du premier antagoniste allostérique agissant sur le domaine VFTD de l’extrémité N-terminale du récepteur mGluR7 potentialise définitivement les observations effectuées sur les souris mGluR7 knock-out quant à la fonction de ce récepteur dans l’anxiété et la dépression. Ensemble, ces données suggèrent que le récepteur mGluR7 est un… Advisors/Committee Members: Weiss, Etienne (thesis director).

Subjects/Keywords: MGluR7; Souris knock-out; Émotion; Cognition; MGluR7; Knockout mice; Emotion; Cognition; 572.8; 573.8; 616.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sansig, G. (2016). Des souris knock-out pour le récepteur métabotrope au glutamate mGluR7 révèlent son rôle dans la cognition et les émotions : Knockout mice for the metabotropic glutamate receptor mGluR7 reveal its role in cognition and emotions. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2016STRAJ018

Chicago Manual of Style (16^th Edition):

Sansig, Gilles. “Des souris knock-out pour le récepteur métabotrope au glutamate mGluR7 révèlent son rôle dans la cognition et les émotions : Knockout mice for the metabotropic glutamate receptor mGluR7 reveal its role in cognition and emotions.” 2016. Doctoral Dissertation, Université de Strasbourg. Accessed January 18, 2021. http://www.theses.fr/2016STRAJ018.

MLA Handbook (7^th Edition):

Sansig, Gilles. “Des souris knock-out pour le récepteur métabotrope au glutamate mGluR7 révèlent son rôle dans la cognition et les émotions : Knockout mice for the metabotropic glutamate receptor mGluR7 reveal its role in cognition and emotions.” 2016. Web. 18 Jan 2021.

Vancouver:

Sansig G. Des souris knock-out pour le récepteur métabotrope au glutamate mGluR7 révèlent son rôle dans la cognition et les émotions : Knockout mice for the metabotropic glutamate receptor mGluR7 reveal its role in cognition and emotions. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2016. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2016STRAJ018.

Council of Science Editors:

Sansig G. Des souris knock-out pour le récepteur métabotrope au glutamate mGluR7 révèlent son rôle dans la cognition et les émotions : Knockout mice for the metabotropic glutamate receptor mGluR7 reveal its role in cognition and emotions. [Doctoral Dissertation]. Université de Strasbourg; 2016. Available from: http://www.theses.fr/2016STRAJ018

21. 林, 亜由美. Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1(Got1l1), a putative aspartate racemase? : アスパラギン酸ラセマーゼとされる Got1ｌ1 は、本当に D-アスパラギン酸を合成するのか？.

Degree: 博士（医学）, 2016, University of Toyama / 富山大学

URL: http://hdl.handle.net/10110/13665 ; http://dx.doi.org/10.15099/00005039

富山大学・富生命博乙第5号・林亜由美・2015/01/22

Amino Acids. 2015 Jan;47(1):79-86. doi: 10.1007/s00726-014-1847-3.に掲載。出版社版はhttp://link.springer.com/article/10.1007%2Fs00726-014-1847-3（オープンアクセス）

2014

Subjects/Keywords: Glutamic-oxaloacetic transaminase-1 like 1; D-Aspartate; Knockout mice; Testis; Hippocampus; Recombinant protein expression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

林, �. (2016). Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1(Got1l1), a putative aspartate racemase? : アスパラギン酸ラセマーゼとされる Got1ｌ1 は、本当に D-アスパラギン酸を合成するのか？. (Thesis). University of Toyama / 富山大学. Retrieved from http://hdl.handle.net/10110/13665 ; http://dx.doi.org/10.15099/00005039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

林, 亜由美. “Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1(Got1l1), a putative aspartate racemase? : アスパラギン酸ラセマーゼとされる Got1ｌ1 は、本当に D-アスパラギン酸を合成するのか？.” 2016. Thesis, University of Toyama / 富山大学. Accessed January 18, 2021. http://hdl.handle.net/10110/13665 ; http://dx.doi.org/10.15099/00005039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

林, 亜由美. “Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1(Got1l1), a putative aspartate racemase? : アスパラギン酸ラセマーゼとされる Got1ｌ1 は、本当に D-アスパラギン酸を合成するのか？.” 2016. Web. 18 Jan 2021.

Vancouver:

林 �. Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1(Got1l1), a putative aspartate racemase? : アスパラギン酸ラセマーゼとされる Got1ｌ1 は、本当に D-アスパラギン酸を合成するのか？. [Internet] [Thesis]. University of Toyama / 富山大学; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10110/13665 ; http://dx.doi.org/10.15099/00005039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

林 �. Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1(Got1l1), a putative aspartate racemase? : アスパラギン酸ラセマーゼとされる Got1ｌ1 は、本当に D-アスパラギン酸を合成するのか？. [Thesis]. University of Toyama / 富山大学; 2016. Available from: http://hdl.handle.net/10110/13665 ; http://dx.doi.org/10.15099/00005039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Hillman, Brandon G. Studies of GluN2C-containing NMDA Receptors in Schizophrenia-like Behaviors and Fear Learning: Relevance to the Glutamate Hypofunction Hypothesis of Schizophrenia.

Degree: PhD, Pharmacology (graduate program), 2012, Creighton University

URL: http://hdl.handle.net/10504/35542

► N-methyl-D-aspartate (NMDA) receptors play an important role in excitatory neurotransmission and mediate synaptic plasticity associated with learning and memory and are postulated to play an… (more)

▼ N-methyl-D-aspartate (NMDA) receptors play an important role in excitatory neurotransmission and mediate synaptic plasticity associated with learning and memory and are postulated to play an important role in numerous neurological diseases such as stroke, traumatic brain injury, alzheimers, dementia and schizophrenia. NMDA receptors are composed to two GluN1 and two GluN2 subunits and the identity of the GluN2 subunit (GluN2A-D) confers unique electrophysiologic and pharmacologic properties to the receptor. The precise role of GluN2C-containing receptors in vivo and in neuropsychiatric disorders such as schizophrenia is poorly understood. The purpose of this study was to determine the global and local in vivo role of GluN2C-containing NMDA receptors with the hypothesis that GluN2C-containing receptors are uniquely involved in the schizophrenia-like phenotypes in rodents and that pharmacologic potentiation of GluN2C-containing receptors is a viable therapeutic strategy in the treatment of schizophrenia. |The first aim of this study was to determine the result of genetic deletion of GluN2C from mice by performing a series of behavioral tests on GluN2C wildtype and knockout mice. Our results show that GluN2C knockout mice behave similar to wildtype in locomotor activity, anxiety, forced-swim induced despair, novel object recognition, pain sensitivity and reference memory. However, GluN2C knockout mice were found to exhibit deficits in fear acquisition and working memory which are two behaviors associated with the schizophrenia-like phenotype in rodents that correlate to the negative symptoms and cognitive deficits in schizophrenia. |The second aim of this study was to then determine the effect of partial or complete deletion of GluN2C subunit on schizophrenia-like behaviors in mice. We found again that GluN2C heterozygous and wildtype mice have normal spontaneous locomotor activity but exhibit hypersensitivity to NMDA channel blocker-induced hyperlocomotion and deficit in working memory versus wildtype counterpart. Additionally, GluN2C deficient mice were found to be more sensitive to social isolation-induced schizophrenia-like behaviors versus wildtype suggesting gene-environment interaction in the GluN2C knockout mouse. Furthermore, we show that systemic potentiation of GluN2C/D-containing receptors with CIQ restores naïve fear acquisition deficit and blocks NMDA antagonist-induced hyperlocomotion and deficit in working memory in GluN2C heterozygous mice. The results of this study show that deficit in function of GluN2C-containing NMDA receptors leads to schizophrenia-like behaviors and that pharmacologic enhancement of GluN2C-containing receptors may serve as a viable therapeutic strategy in the reversal of behavioral and cognitive deficits in schizophrenia. |The third aim of this study builds on the previous result of potentiation of GluN2C-containing receptors by delivering a selective potentiator of GluN2C-containing receptors into the prefrontal cortex (PFC) of wildtype and knockout mice after… Advisors/Committee Members: Dravid, Shashank M. (advisor), Hillman, Brandon G. (cuauthor).

Subjects/Keywords: Schizophrenia – genetics; Receptors, Glutamate – genetics; N-Methylaspartate – genetics; Mental Disorders – genetics; Behavior, Animal; Mice, Knockout

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hillman, B. G. (2012). Studies of GluN2C-containing NMDA Receptors in Schizophrenia-like Behaviors and Fear Learning: Relevance to the Glutamate Hypofunction Hypothesis of Schizophrenia. (Doctoral Dissertation). Creighton University. Retrieved from http://hdl.handle.net/10504/35542

Chicago Manual of Style (16^th Edition):

Hillman, Brandon G. “Studies of GluN2C-containing NMDA Receptors in Schizophrenia-like Behaviors and Fear Learning: Relevance to the Glutamate Hypofunction Hypothesis of Schizophrenia.” 2012. Doctoral Dissertation, Creighton University. Accessed January 18, 2021. http://hdl.handle.net/10504/35542.

MLA Handbook (7^th Edition):

Hillman, Brandon G. “Studies of GluN2C-containing NMDA Receptors in Schizophrenia-like Behaviors and Fear Learning: Relevance to the Glutamate Hypofunction Hypothesis of Schizophrenia.” 2012. Web. 18 Jan 2021.

Vancouver:

Hillman BG. Studies of GluN2C-containing NMDA Receptors in Schizophrenia-like Behaviors and Fear Learning: Relevance to the Glutamate Hypofunction Hypothesis of Schizophrenia. [Internet] [Doctoral dissertation]. Creighton University; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10504/35542.

Council of Science Editors:

Hillman BG. Studies of GluN2C-containing NMDA Receptors in Schizophrenia-like Behaviors and Fear Learning: Relevance to the Glutamate Hypofunction Hypothesis of Schizophrenia. [Doctoral Dissertation]. Creighton University; 2012. Available from: http://hdl.handle.net/10504/35542

23. Sanches, Juliane Cristina Trevisan. Estudo ultraestrutural e imunohistoquímico do estroma uterino durante a gestação de camundongos deficientes em decorim.

Degree: Mestrado, Biologia Celular e Tecidual, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09062009-103722/ ;

►

No presente estudo realizamos uma análise ultraestrutural e imuhistoquímica da organização das fibrilas de colágeno no endométrio durante a gestação de camundongos silvestres e deficientes… (more)

▼

No presente estudo realizamos uma análise ultraestrutural e imuhistoquímica da organização das fibrilas de colágeno no endométrio durante a gestação de camundongos silvestres e deficientes em decorim. Os resultados mostraram que as fibrilas de colágeno em ambos os genótipos sofrem grande variação de forma e tamanho. Observou-se variação significante, na percentagem de distribuição dos diâmetros das fibrilas de colágeno existentes na região decidualizada em ambos os genótipos, porém foi maior nos animais Dcn-/-. Estes animais também apresentam maior percentagem de fibrilas finas quando comparados aos animais Dcn+/+. Observamos ainda que biglicam é expresso no endométrio não decidualizado dos animais Dcn-/-, no 3º dia de gestação. A expressão de lumicam mostrou-se nítida no estroma decidualizado e não decidualizado nos animais Dcn-/- no 7º dia de gestação e foi ausente nos animais Dcn-/-. Estes resultados mostraram que a ausência do decorim promove distúrbios no processo de agregação lateral das fibrilas espessas de colágeno.

The present study is an ultrastructural investigation into the organization of collagen fibrils in the pregnant endometrium of wild-type and decorin-deficient mice. Our results showed that collagen fibrils from both genotypes present a great variability of shape and size in cross section. Significant variation in the diameter of collagen fibrils exists in the decidualized endometrium from both groups of animals. In the decidualized endometrium, the diameter of collagen fibrils increases in both genotypes were higher in Dcn-/- than in Dcn+/+ animals. In the Dcn-/- animals the percentage of thin fibrils with diameter is also higher, when compared with Dcn+/+ animals. We also showed that Bgn is expressed in the non decidualized endometrium in the Dcn-/- animals, on day 3 of pregnancy. The expression of lumican showed a very sharp labeling in the decidualized stroma from day 7, and in the non decidualized estroma from Dcn-/- animals. These results suggest that the deficience of decorin may play a role on collagen fibrillogenesis in different stages of pregnancy.

Advisors/Committee Members: Oliveira, Sergio Ferreira de.

Subjects/Keywords: Camundongos nocautes; Colágeno; Collagen; Decidua; Decídua; Decorim; Decorim; Endométrio; Endometrium; Knockout mice; Ultraestrutura; Ultrastructural

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sanches, J. C. T. (2009). Estudo ultraestrutural e imunohistoquímico do estroma uterino durante a gestação de camundongos deficientes em decorim. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09062009-103722/ ;

Chicago Manual of Style (16^th Edition):

Sanches, Juliane Cristina Trevisan. “Estudo ultraestrutural e imunohistoquímico do estroma uterino durante a gestação de camundongos deficientes em decorim.” 2009. Masters Thesis, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09062009-103722/ ;.

MLA Handbook (7^th Edition):

Sanches, Juliane Cristina Trevisan. “Estudo ultraestrutural e imunohistoquímico do estroma uterino durante a gestação de camundongos deficientes em decorim.” 2009. Web. 18 Jan 2021.

Vancouver:

Sanches JCT. Estudo ultraestrutural e imunohistoquímico do estroma uterino durante a gestação de camundongos deficientes em decorim. [Internet] [Masters thesis]. University of São Paulo; 2009. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09062009-103722/ ;.

Council of Science Editors:

Sanches JCT. Estudo ultraestrutural e imunohistoquímico do estroma uterino durante a gestação de camundongos deficientes em decorim. [Masters Thesis]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09062009-103722/ ;

24. Kyrargyri, Vasiliki. Ο ρόλος του μεταγραφικού παράγοντα NF-κΒ στη φυσιολογία των νευρώνων και σε διαταραχές του κεντρικού νευρικού συστήματος.

Degree: 2014, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/41819

►

Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning… (more)

▼

Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning and memory is not fully understood. In this study, we used mice with partial depletion of IκB kinase β, the main activating kinase in the inducible NF-κB pathway, selectively in myeloid lineage cells (mIKKβKO) or excitatory neurons (nIKKβKO) to measure synaptic strength at hippocampal Schaffer collaterals during long term potentiation (LTP) and instrumental conditioning in alert behaving individuals. Resting microglial cells in mIKKβKO mice showed less Iba1-immunoreactivity, and brain IL-1β mRNA levels were selectively reduced compared to controls. Measurement of field excitatory postsynaptic potentials (fEPSPs) evoked by stimulation of the CA3-CA1 synapse in mIKKβKO mice showed higher facilitation in response to paired pulses and enhanced LTP following high frequency stimulation. In contrast, nIKKβKO mice showed normal basic synaptic transmission and LTP induction but impairments in late LTP. To understand the consequences of such impairments in synaptic plasticity for learning and memory, we measured CA1 fEPSPs in behaving mice during instrumental conditioning. IKKβ was not needed in either microglia or neurons for mice to learn lever-pressing (appetitive behavior) to obtain food (consummatory behavior) but was required in both for modification of their hippocampus-dependent appetitive, not consummatory behavior. Our results show that microglia, through IKKβ and therefore NF-κB activity, regulate hippocampal synaptic plasticity and that both microglia and neurons, through IKKβ, are necessary for animals to modify hippocampus-driven behavior during associative learning.

Τα μικρογλοιακά κύτταρα αποτελούν τα τοπικά ανοσοποιητικά κύτταρα του Κεντρικού Νευρικού Συστήματος (ΚΝΣ) και μια πλούσια πηγή νευροδραστικών μεσολαβητών, ωστόσο η συνεισφορά τους στις φυσιολογικές διαδικασίες του εγκεφάλου, όπως η συναπτική πλαστικότητα, η μνήμη και η μάθηση δεν έχει κατανοηθεί προς το παρόν. Στην εργασία αυτή, χρησιμοποιήσαμε επίμυες με μερική απάλειψη της ΙκB κινάσης β, την βασικότερη κινάση ενεργοποίησης του επαγόμενου NF-κB μονοπατιού, επιλεκτικά σε κύτταρα μυελοειδικής προέλευσης (mIKKβKO) ή σε διεγερτικούς νευρώνες (nIKKβKO) με σκοπό να μετρήσουμε την αποτελεσματικότητα της συναπτικής διαβίβασης της οδού των παράπλευρων κλάδων Schaffer του ιππόκαμπου κατά τη διάρκεια της μακρόχρονης ενδυνάμωσης (LTP) και της συντελεστικής εξαρτημένης μάθησης. Τα μη ενεργοποιημένα μικρογλοιακά κύτταρα των mIKKβKO επιμύων έδειξαν μειωμένη Iba1-ανοσολογική έκφραση, και χαμηλότερο επίπεδο έκφρασης του mRNA της IL-1β στον εγκέφαλό τους σε σχέση με τους επιμύες ελέγχου. Μετρήσεις των διεγερτικών μετασυναπτικών δυναμικών πεδίου (fEPSPs) που προκλήθηκαν με διέγερση της CA3-CA1 σύναψης στους mIKKβKO επίμυες έδειξαν αυξημένη συναπτική ενδυνάμωση σε απόκριση διπλού διεγερτικού παλμού και ενισχυμένη LTP κατόπιν διέγερσης υψηλής συχνότητας. Σε αντίθεση, οι nIKKβKO επίμυες…

Subjects/Keywords: Μικρογλοία; Διαγονιδιακά ποντίκια; Συναπτική πλαστικότητα; Συμπεριφορά; Microglia; NF-κB; Knockout mice; Synaptic plasticity; Behavior

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kyrargyri, V. (2014). Ο ρόλος του μεταγραφικού παράγοντα NF-κΒ στη φυσιολογία των νευρώνων και σε διαταραχές του κεντρικού νευρικού συστήματος. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/41819

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kyrargyri, Vasiliki. “Ο ρόλος του μεταγραφικού παράγοντα NF-κΒ στη φυσιολογία των νευρώνων και σε διαταραχές του κεντρικού νευρικού συστήματος.” 2014. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 18, 2021. http://hdl.handle.net/10442/hedi/41819.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kyrargyri, Vasiliki. “Ο ρόλος του μεταγραφικού παράγοντα NF-κΒ στη φυσιολογία των νευρώνων και σε διαταραχές του κεντρικού νευρικού συστήματος.” 2014. Web. 18 Jan 2021.

Vancouver:

Kyrargyri V. Ο ρόλος του μεταγραφικού παράγοντα NF-κΒ στη φυσιολογία των νευρώνων και σε διαταραχές του κεντρικού νευρικού συστήματος. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10442/hedi/41819.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kyrargyri V. Ο ρόλος του μεταγραφικού παράγοντα NF-κΒ στη φυσιολογία των νευρώνων και σε διαταραχές του κεντρικού νευρικού συστήματος. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2014. Available from: http://hdl.handle.net/10442/hedi/41819

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

25. Alexander, Peter Barton. Requirement of a High-Flux Metabolic State for Mouse Embryonic Stem Cell Self-Renewal.

Degree: 2010, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/798

► Unbiased profiling of global metabolite levels has revealed that cultured mouse embryonic stem (ES) cells exist in a unique metabolic state. Metabolites fluctuating dramatically in… (more)

Subjects/Keywords: Threonine; Embryonic Stem Cells; Mice, Knockout

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APA (6^th Edition):

Alexander, P. B. (2010). Requirement of a High-Flux Metabolic State for Mouse Embryonic Stem Cell Self-Renewal. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/798

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alexander, Peter Barton. “Requirement of a High-Flux Metabolic State for Mouse Embryonic Stem Cell Self-Renewal.” 2010. Thesis, University of Texas Southwestern Medical Center. Accessed January 18, 2021. http://hdl.handle.net/2152.5/798.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alexander, Peter Barton. “Requirement of a High-Flux Metabolic State for Mouse Embryonic Stem Cell Self-Renewal.” 2010. Web. 18 Jan 2021.

Vancouver:

Alexander PB. Requirement of a High-Flux Metabolic State for Mouse Embryonic Stem Cell Self-Renewal. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2152.5/798.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alexander PB. Requirement of a High-Flux Metabolic State for Mouse Embryonic Stem Cell Self-Renewal. [Thesis]. University of Texas Southwestern Medical Center; 2010. Available from: http://hdl.handle.net/2152.5/798

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

26. Adusei, Daniel C. Early Developmental Alterations in GABAergic Protein Expression in Fragile X Knockout Mice.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/25402

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The purpose of this study was to examine the expression of GABAergic proteins in Fmr1 knockout mice during brain maturation and to assess behavioural changes… (more)

Subjects/Keywords: Fragile X syndrome; Fmr1 knockout mice; GABAergic system; GABA(A) receptor; 0572; 0317

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Adusei, D. C. (2010). Early Developmental Alterations in GABAergic Protein Expression in Fragile X Knockout Mice. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25402

Chicago Manual of Style (16^th Edition):

Adusei, Daniel C. “Early Developmental Alterations in GABAergic Protein Expression in Fragile X Knockout Mice.” 2010. Masters Thesis, University of Toronto. Accessed January 18, 2021. http://hdl.handle.net/1807/25402.

MLA Handbook (7^th Edition):

Adusei, Daniel C. “Early Developmental Alterations in GABAergic Protein Expression in Fragile X Knockout Mice.” 2010. Web. 18 Jan 2021.

Vancouver:

Adusei DC. Early Developmental Alterations in GABAergic Protein Expression in Fragile X Knockout Mice. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1807/25402.

Council of Science Editors:

Adusei DC. Early Developmental Alterations in GABAergic Protein Expression in Fragile X Knockout Mice. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25402

Univerzitet u Beogradu

27. Milosavljević, Petar D., 1960-. Uloga galektina-3 u razvoju periapeksnih inflamatornih lezija kod miša.

Degree: Fakultet veterinarske medicine, 2014, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:6864/bdef:Content/get

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Veterinarska medicina - Veterinarska patologija, imunologija / Veterinary medicine - Animal pathology, Immunology

Galektini su evolutivno veoma stari plejotropni proteini sa različitim funkcijama u urođenom… (more)

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Veterinarska medicina - Veterinarska patologija, imunologija / Veterinary medicine - Animal pathology, Immunology

Galektini su evolutivno veoma stari plejotropni proteini sa različitim funkcijama u urođenom i stečenom imunitetu. Do danas je kod sisara u mnogim tkivima, intra i ekstraćelijski, otkriveno ukupno 15 galektina sa nizom uloga u prepoznavanju patogena i finoj regulaciji imunološkog odgovora. Galektini se ponašaju kao receptori (PRR) za prepoznavanje zajedničkih molekularnih obrazaca mikroorganizama (PAMP) koji aktiviraju urođeni imunološki odgovor. Galektin-3 je himerični član familije galektina koji ispoljava proinflamatorno ali i imunoregulatorno delovanje. Snažan je aktivator leukocita i delujući kao adhezivni molekul važan je za njihovu direktnu adheziju za endotelne ćelije i proces ekstravazacije. Cilj ovog istraživanja bio je ispitivanje uticaja galektina-3 na razvoj eksperimentalno izazvanih periapeksnih inflamatornih lezija kod galektin-3 nokaut miševa mesec dana, šest nedelja i četiri meseca od izazivanja oboljenja. Periapeksne inflamatorne lezije izazvane su otvaranjem komunikacije između usne duplje i zubne pulpe stomatološkom burgijom. Prodor patogenih anaeroba dentalnog plaka P.gingivalis, P.intermedia, F.nucleatum, A.actinomycetemcomitans i drugih u zubnu pulpu vodi do razvoja snažnog periodontitisa. Lipopolisaharid (LPS) P.gingivalis, ligand TLR2 i TLR4, stimuliše lučenje različitih proinflamatornih i antiinflamatornih citokina od strane antigen prezentujućih ćelija (APĆ), pre svega makrofaga i dendritskih ćelija (DĆ). Galektin-3 je negativni regulator funkcije LPS pa je sekrecija citokina od strane makrofaga galektin-3 nokaut miševa povećana u odnosu na miševe divljeg tipa. Značajno smanjen broj neutrofilnih granulocita u periapeksnim inflamatornim infiltratima ovih životinja u odnosu na divlji tip, uz približno jednak broj makrofaga i limfocita, ukazuje da nedostatak ovog molekula smanjuje intenzitet inflamatornih procesa u periodoncijumu. Neutrofilni granulociti su najbrojnije ćelije u periapeksnim inflamatornim lezijama miševa divljeg tipa a makrofagi kod galektin-3 negativnih nokaut miševa. Real-time PCR detekcijom ekspresije gena za proinflamatorne i antiinflamatorne citokine na nivou informacione RNK (iRNK), mesec dana od izazivanja oboljenja utvrđeno je značajno povećanje ekspresije gena za proinflamatorne citokine IL-1β, IL-6, IL-17, IL-12, IL-23, IL-4 i I L-18 kod galektin-3 nokaut miševa u odnosu na miševe divljeg tipa i značajno smanjenje ekspresije gena za TNFα, IL-1α, IFNγ, IL-21, IL-10, i TGFβ...

Advisors/Committee Members: Jovanović, Milijan, 1951-.

Subjects/Keywords: Galectin-3; periapical inflammatory lesions; knockout mice; pathohistology; real-time PCR; cytokine gene expression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Milosavljević, Petar D., 1. (2014). Uloga galektina-3 u razvoju periapeksnih inflamatornih lezija kod miša. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:6864/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Milosavljević, Petar D., 1960-. “Uloga galektina-3 u razvoju periapeksnih inflamatornih lezija kod miša.” 2014. Thesis, Univerzitet u Beogradu. Accessed January 18, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:6864/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Milosavljević, Petar D., 1960-. “Uloga galektina-3 u razvoju periapeksnih inflamatornih lezija kod miša.” 2014. Web. 18 Jan 2021.

Vancouver:

Milosavljević, Petar D. 1. Uloga galektina-3 u razvoju periapeksnih inflamatornih lezija kod miša. [Internet] [Thesis]. Univerzitet u Beogradu; 2014. [cited 2021 Jan 18]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:6864/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Milosavljević, Petar D. 1. Uloga galektina-3 u razvoju periapeksnih inflamatornih lezija kod miša. [Thesis]. Univerzitet u Beogradu; 2014. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:6864/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manitoba

28. Adhikari Bagchi, Rushita. Transcriptional regulation of cardiac extracellular matrix gene expression and fibroblast phenotype by scleraxis.

Degree: Physiology and Pathophysiology, 2015, University of Manitoba

URL: http://hdl.handle.net/1993/31243

► Cardiac fibrosis contributes to heart failure by dramatically impairing cardiac function, increasing patient morbidity and mortality. The primary fibrillar collagen expressed in the heart is… (more)

▼ Cardiac fibrosis contributes to heart failure by dramatically impairing cardiac function, increasing patient morbidity and mortality. The primary fibrillar collagen expressed in the heart is type I, and increased collagen synthesis is the hallmark of cardiac fibrosis. Our laboratory has shown that the transcription factor scleraxis is sufficient to regulate the gene encoding collagen Iα2. The present thesis identifies and focuses on three key functions of scleraxis in the heart. First, we show that scleraxis is required for production of the cardiac extracellular matrix. Using in vitro and in vivo models, we observed a significant upregulation/reduction of matrix genes in response to induction/loss of scleraxis gene function respectively. In fact, scleraxis overexpression was sufficient to rescue matrix synthesis in scleraxis-null cells. In a murine model of cardiac pressure overload, scleraxis gene deletion blunted the induction of fibrotic collagen gene expression. Second, we provide evidence that scleraxis governs fibroblast-myofibroblast phenotype transition and fibroblast number. Scleraxis gene induction promoted cardiac myofibroblast phenoconversion while knockdown reduced myofibroblast marker gene expression. Scleraxis exerts direct transcriptional control on the a-smooth muscle actin gene-an established marker of myofibroblasts. Scleraxis null mice exhibited a dramatic reduction in cardiac fibroblast numbers- this is attributed to impairment of the epithelial-to-mesenchymal transition program which was marked by a corresponding loss of mesenchymal markers and increased epithelial markers. Loss-of-function experiments using primary cardiac proto-myofibroblasts recapitulated this paradigm, whereas scleraxis gene induction showed a reciprocal effect on mesenchymal markers. Third, data from this study supports the required role of scleraxis in the TGFb/Smad signaling pathway. Scleraxis is strongly upregulated by the potent pro-fibrotic cytokine TGFb, and works synergistically with the canonical Smad signaling pathway to increase Col1a2 expression by cardiac fibroblasts and myofibroblasts. Smad3 induced expression of the fibrillar collagens – an effect that was significantly attenuated following scleraxis knockdown. Smad3 binding to the Col1a2 gene promoter was significantly reduced in scleraxis null hearts. This study involved a comprehensive series of in vitro and in vivo experiments, and is the first to identify scleraxis as a key regulator of multiple fibroblast functions and a potential future target for therapeutic intervention in cardiac fibrosis. Advisors/Committee Members: Czubryt, Michael Paul (Physiology and Pathophysiology) (supervisor), Kardami, Elissavet (Physiology and Pathophysiology).

Subjects/Keywords: fibroblast; myofibroblast; transcription; gene regulation; transgenic mice; extracellular matrix; conditional knockout; fibrosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Adhikari Bagchi, R. (2015). Transcriptional regulation of cardiac extracellular matrix gene expression and fibroblast phenotype by scleraxis. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/31243

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Adhikari Bagchi, Rushita. “Transcriptional regulation of cardiac extracellular matrix gene expression and fibroblast phenotype by scleraxis.” 2015. Thesis, University of Manitoba. Accessed January 18, 2021. http://hdl.handle.net/1993/31243.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Adhikari Bagchi, Rushita. “Transcriptional regulation of cardiac extracellular matrix gene expression and fibroblast phenotype by scleraxis.” 2015. Web. 18 Jan 2021.

Vancouver:

Adhikari Bagchi R. Transcriptional regulation of cardiac extracellular matrix gene expression and fibroblast phenotype by scleraxis. [Internet] [Thesis]. University of Manitoba; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1993/31243.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adhikari Bagchi R. Transcriptional regulation of cardiac extracellular matrix gene expression and fibroblast phenotype by scleraxis. [Thesis]. University of Manitoba; 2015. Available from: http://hdl.handle.net/1993/31243

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

29. DeGorter, Marianne K. Statin Transport by Hepatic Organic Anion-Transporting Polypeptides (OATPs).

Degree: 2012, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/691

► Drug transport proteins are important determinants of drug absorption, tissue accumulation, and elimination from the body, and there is growing appreciation for the contribution of… (more)

▼ Drug transport proteins are important determinants of drug absorption, tissue accumulation, and elimination from the body, and there is growing appreciation for the contribution of altered drug transporter function to interindividual variability in drug response. The organic anion-transporting polypeptides (OATPs/SLCO) are uptake transporters with broad substrate specificity. Notably, the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors, or statins, are commonly prescribed OATP substrates. The OATP1B subfamily, expressed predominantly in the liver, is of particular importance to statins, which require hepatic entry to exert their low-density lipoprotein cholesterol lowering effect. We aimed to identify molecular determinants of substrate specificity in the human OATP1B subfamily in vitro, and found three regions required for transport of a non-statin substrate, cholecystokinin-8, thus improving our understanding of OATP1B transport mechanism. We employed Oatp1b2-/- mice to model reduced OATP1B function in humans, and observed liver-to-plasma ratios of atorvastatin and rosuvastatin were lower in Oatp1b2-/- mice compared with wild-type animals, further emphasizing the importance of this OATP subfamily to hepatic drug uptake. One challenge to statin therapy is the risk for muscle toxicity associated with elevated systemic statin exposure. We assessed intraindividual variability in statin pharmacokinetics in human subjects, and found a correlation in exposure to atorvastatin and simvastatin, which are both metabolized by cytochrome P450 3A (CYP3A). In contrast, atorvastatin and simvastatin exposure were not correlated with rosuvastatin, a statin that is transported but not significantly metabolized, thus illustrating the interplay between transport and metabolism that influences statin pharmacokinetics. Though numerous clinical trials have investigated statin effectiveness, interindividual variability in statin pharmacokinetics in a clinical setting is not well understood. We characterized atorvastatin and rosuvastatin concentration in 299 patients at London Health Sciences Center, and observed 45-fold variability. Genetic variants in SLCO1B1 and ABCG2 were associated with rosuvastatin concentration. Atorvastatin concentration was associated with SLCO1B1 variants and with 4β-hydroxycholesterol concentration, a marker of CYP3A activity. Lathosterol, a marker of HMG-CoA reductase function, was not associated with statin concentration in our population. Taken together, these studies further our understanding of OATP function, both in vivo and in vitro, and the contribution of OATPs to pharmacokinetics and drug response.

Subjects/Keywords: Organic anion-transporting polypeptides; pharmacogenetics; drug transporters; transporter knockout mice; statin transport; statin pharmacokinetics; Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

DeGorter, M. K. (2012). Statin Transport by Hepatic Organic Anion-Transporting Polypeptides (OATPs). (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

DeGorter, Marianne K. “Statin Transport by Hepatic Organic Anion-Transporting Polypeptides (OATPs).” 2012. Thesis, University of Western Ontario. Accessed January 18, 2021. https://ir.lib.uwo.ca/etd/691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

DeGorter, Marianne K. “Statin Transport by Hepatic Organic Anion-Transporting Polypeptides (OATPs).” 2012. Web. 18 Jan 2021.

Vancouver:

DeGorter MK. Statin Transport by Hepatic Organic Anion-Transporting Polypeptides (OATPs). [Internet] [Thesis]. University of Western Ontario; 2012. [cited 2021 Jan 18]. Available from: https://ir.lib.uwo.ca/etd/691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

DeGorter MK. Statin Transport by Hepatic Organic Anion-Transporting Polypeptides (OATPs). [Thesis]. University of Western Ontario; 2012. Available from: https://ir.lib.uwo.ca/etd/691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

30. Gibbins, Matthew Thomas George. Metabolic and vascular effects of thiosulfate sulfurtransferase deletion.

Degree: PhD, 2018, University of Edinburgh

URL: http://hdl.handle.net/1842/31558

► Hydrogen sulfide (H2S), is a gasotransmitter with several key roles in metabolism and vascular function. The effects of H2S are dependent on concentration and target… (more)

▼ Hydrogen sulfide (H2S), is a gasotransmitter with several key roles in metabolism and vascular function. The effects of H2S are dependent on concentration and target organ. For example, increased H2S concentrations impair liver metabolic function but protect against vascular dysfunction and atherosclerosis. Thiosulfate sulfurtransferase (TST), a nuclear encoded mitochondrial matrix enzyme, is proposed to be a component of the sulfide oxidising unit (SOU) which metabolises H2S. Preliminary data has shown that Tst deletion in mice (Tst-/-) increases circulating H2S levels measured in whole blood. Therefore, it was hypothesised that Tst-/- mice would exhibit worsened metabolic function in the liver but also protection of vascular function under conditions of vascular stress e.g. atherosclerosis. Liver metabolism was assessed by extensive metabolic phenotyping of Tst-/-mice fed control diet and in conditions of metabolic dysfunction induced by a high fat diet (HFD). Tst deletion altered glucose metabolism in mice; gluconeogenesis was increased in liver from Tst-/-mice fed control diet. Glucose intolerance in HFD-fed Tst-/-mice was also more severe than HFDfed C57BL/6 controls. In vitro metabolic investigations in primary hepatocytes isolated from Tst-/-mice demonstrated that mitochondrial ATP-linked and leak respiration were increased compared to controls. The effect of Tst deletion on vascular function was investigated in Tst- /-mice fed control or HFD using myography. Tst deletion did not alter vessel function when mice were maintained on a normal diet. HFD feeding (20 weeks) reduced maximal vessel constriction in the presence of endothelial nitric oxide synthase and cyclooxygenase inhibitors in C57BL/6 aorta. However, in Tst-/-mice fed HFD there was no reduction in maximal constriction suggesting a protective action of Tst deletion. The effects of Tst deletion on atherosclerotic lesions was investigated by generating double knock-out (DKO) mice by deletion of the Tst gene in ApoE-/- mice and (ApoE-/-Tst-/-). Atherosclerotic lesion formation was accelerated by feeding mice a western diet. Within the brachiocephalic branch lesion volume and total vessel volume were reduced in DKO mice fed western diet for 12 weeks, indicating that Tst deletion reduced lesion formation. Plasma cholesterol was reduced in DKO mice compared to ApoE-/- controls and a trend towards reduced systolic blood pressure was also noted. Overall this work supported the hypothesis that Tst deletion engenders metabolic dysfunction but vascular protection. The findings are consistent with the reported effects of increased H2S signalling. Overall inhibition of TST represents a novel target for treatment of atherosclerosis, with the caveat that glycaemia may be worsened due to hepatic metabolic dysfunction.

Subjects/Keywords: 616.3; hydrogen sulfide; Thiosulfate sulfurtransferase; Tst knockout mice; liver function; double knock-out; atherosclerosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gibbins, M. T. G. (2018). Metabolic and vascular effects of thiosulfate sulfurtransferase deletion. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/31558

Chicago Manual of Style (16^th Edition):

Gibbins, Matthew Thomas George. “Metabolic and vascular effects of thiosulfate sulfurtransferase deletion.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed January 18, 2021. http://hdl.handle.net/1842/31558.

MLA Handbook (7^th Edition):

Gibbins, Matthew Thomas George. “Metabolic and vascular effects of thiosulfate sulfurtransferase deletion.” 2018. Web. 18 Jan 2021.

Vancouver:

Gibbins MTG. Metabolic and vascular effects of thiosulfate sulfurtransferase deletion. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1842/31558.

Council of Science Editors:

Gibbins MTG. Metabolic and vascular effects of thiosulfate sulfurtransferase deletion. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/31558

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Open Access Theses and Dissertations