subject:(Knock)

Colorado State University

1. Wise, Daniel M. Investigation into producer gas utilization in high performance natural gas engines.

Degree: PhD, Mechanical Engineering, 2013, Colorado State University

► A wide range of fuels are used in industrial gas fueled engines including well-head gas, pipeline natural gas, producer gas, coal gas, digester gas, landfill… (more)

▼ A wide range of fuels are used in industrial gas fueled engines including well-head gas, pipeline natural gas, producer gas, coal gas, digester gas, landfill gas, and liquefied petroleum gas. Many industrial gas fueled engines operate both at high power density for increased efficiency and at ultra-lean air-fuel ratios for low NOx emissions. These two conditions require that engine operation occurs in a narrow air-fuel ratio band between the limits of misfire and the initiation of knock. The ability to characterize these limits for a given fuel is essential for efficient and effective engine operation. This work pursues two primary research objectives: (1) to characterize producer gas blends by developing prognostic tools with respect to a given blend's resistance to knock and (2) to develop a process to determine knock onset for a given fuel gas through direct indication from pressure transducer data at varied air-fuel ratios (ranging from stoichiometric to ultra-lean) as well as varied intake conditions (ranging from naturally aspirated to boosted intake pressures replicating turbocharged engines) and to quantitatively characterize the knock event using discreet and repeatable metrics derived from the analysis of the data. Methane number determination for natural gas blends is traditionally performed with research engines at stoichiometric conditions where the onset of knock is identified through subjective audible indication. To more closely replicate the operating conditions of a typical industrial engine, a Cooperative Fuel Research (CFR F2) engine is modified for boosted fuel/air intake and variable exhaust back pressure (to simulate turbocharger operation) with the incorporation of piezoelectric pressure transducers at the cylinder head to allow quantitative analysis of cylinder pressure conditions and transients precursive to, during, and following a knock event of varying magnitude. The interpretation of this data provides for evaluation of unique analytical methods to quantify and characterize engine knock under these conditions. In the course of this study an objective and consistent method for measuring methane number is developed, measured methane number for a total of 35 producer gas blends is provided, and a prognostic tool for predicting methane number, utilizing neural networks, is presented. Advisors/Committee Members: Olsen, Daniel B. (advisor), Caille, Gary (committee member), Marchese, Anthony (committee member), Sharvelle, Sybil (committee member).

Subjects/Keywords: knock; producer gas; methane number

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APA (6^th Edition):

Wise, D. M. (2013). Investigation into producer gas utilization in high performance natural gas engines. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/78876

Chicago Manual of Style (16^th Edition):

Wise, Daniel M. “Investigation into producer gas utilization in high performance natural gas engines.” 2013. Doctoral Dissertation, Colorado State University. Accessed April 15, 2021. http://hdl.handle.net/10217/78876.

MLA Handbook (7^th Edition):

Wise, Daniel M. “Investigation into producer gas utilization in high performance natural gas engines.” 2013. Web. 15 Apr 2021.

Vancouver:

Wise DM. Investigation into producer gas utilization in high performance natural gas engines. [Internet] [Doctoral dissertation]. Colorado State University; 2013. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/10217/78876.

Council of Science Editors:

Wise DM. Investigation into producer gas utilization in high performance natural gas engines. [Doctoral Dissertation]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/78876

2. Parameshwara, Vikram. Numerical study of autoignition of fuel-air mixtures at elevated temperatures and pressures .

Degree: Chalmers tekniska högskola / Institutionen för mekanik och maritima vetenskaper, 2021, Chalmers University of Technology

URL: http://hdl.handle.net/20.500.12380/302265

► In a conventional spark-ignition (SI) engine the fuel-air mixture is injected into the cylinder where it is mixed with the residual gasses and compressed. Under… (more)

▼ In a conventional spark-ignition (SI) engine the fuel-air mixture is injected into the cylinder where it is mixed with the residual gasses and compressed. Under normal operation, combustion takes place at the end of the compression stroke, the mixture is ignited by a spark, flame kernel grows, turbulent flame develops and propagates to the walls where it is quenched. As we strive for better thermal efficiency, the compression ratio is the ideal parameter to increase. However, a phenomenon called knock occurs at elevated temperatures and pressures and impedes the improvements to thermal efficiency by simply increasing the compression ratio. Knock occurs because of auto-ignition initiated locally in hot spots in the unburnt fuel-air mixture ahead of the advancing flame front. It deteriorates the working characteristics of the engine and drastically reduces its durability. Previously, most of the research into knock was carried out experimentally. The recent developments in computer hardware, software, and dedicated chemical kinetic mechanisms that can accurately model the behaviour of gasoline have made it possible to calculate the autoignition of fuels with acceptable accuracy. The main objective of this project is to study the autoignition behaviour of various fuel-air mixtures at elevated temperatures and pressures using the aforementioned chemical kinetic mechanisms. The autoignition of commercially available fuels is the focus of this thesis. The chemical kinetic mechanisms chosen are from RWTH Aachen University, King Abdullah University of Science, and Technology and Lawrence Livermore National Laboratory. Using the software CHEMKIN-Pro, these mechanisms are adopted to simulate the ignition delay times of iso-octane, n-heptane, and primary reference fuel blended with ethanol (PRF-E) at various temperatures, pressures, and equivalence ratios (fuel-air ratio). The calculated values of ignition delay times are compared with the experimental data available for the same input parameters and the chemical mechanisms from Aachen university is chosen based on the accuracy of the results and the speed of computations. Using the selected mechanism, ignition delay times for E10 (fuel of RON95 with 10% ethanol by volume), 95 unleaded (RON95), and 98 unleaded (RON98) are calculated. The knock prediction model by Kalghatgi et al., 2017 is used as the criterion for suggesting the best operating range temperature range of the fuels to avoid knock in an engine. Using MATLAB and Excel, graphs for the calculated ignition delay times vs temperature are plotted to study the dependence of various input parameters (temperature, pressure, equivalence ratio, and fuel composition). The following results are observed: • The ignition delay times reduce with an increase in the input pressure at a particular temperature. • The ignition delay times decrease with an increase in equivalence ratio (ϕ). • The knock resistance of a fuel increases with an increase in the RON of a fuel. • The ignition delay times reduce with an increase in…

Subjects/Keywords: Knock; Auto-ignition; Ignition delay

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APA (6^th Edition):

Parameshwara, V. (2021). Numerical study of autoignition of fuel-air mixtures at elevated temperatures and pressures . (Thesis). Chalmers University of Technology. Retrieved from http://hdl.handle.net/20.500.12380/302265

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Parameshwara, Vikram. “Numerical study of autoignition of fuel-air mixtures at elevated temperatures and pressures .” 2021. Thesis, Chalmers University of Technology. Accessed April 15, 2021. http://hdl.handle.net/20.500.12380/302265.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Parameshwara, Vikram. “Numerical study of autoignition of fuel-air mixtures at elevated temperatures and pressures .” 2021. Web. 15 Apr 2021.

Vancouver:

Parameshwara V. Numerical study of autoignition of fuel-air mixtures at elevated temperatures and pressures . [Internet] [Thesis]. Chalmers University of Technology; 2021. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/20.500.12380/302265.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Parameshwara V. Numerical study of autoignition of fuel-air mixtures at elevated temperatures and pressures . [Thesis]. Chalmers University of Technology; 2021. Available from: http://hdl.handle.net/20.500.12380/302265

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brunel University

3. Dingle, Simon Frederick. Lubricant induced pre-ignition in an optical spark-ignition engine.

Degree: PhD, 2014, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/14026 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.824232

► This work focuses on the introduction of lubricant into the combustion chamber and the effect that this has on pre-ignition. Apparently for the first time,… (more)

▼ This work focuses on the introduction of lubricant into the combustion chamber and the effect that this has on pre-ignition. Apparently for the first time, the work presented provides detailed full-bore optical data for lubricant induced pre-ignition and improves understanding of the super-knock phenomena that affects modern downsized gasoline engines. A new single-cylinder optical research engine was designed, fabricated and installed. The research engine featured poppet valves and a unique four-valve layout. At the same time, complete optical access to the piston crown and top-land crevice region was maintained in a disc-shaped combustion chamber. The engine featured a novel optical window clamping arrangement to withstand peak knocking pressures in excess of 120bar; a capability that is believed to be unique amongst full-bore optical engines at present. A system was designed for deliberately introducing a controlled sample of lubricant into the hottest region of the combustion chamber. It was found that, through injecting a small sample of lubricant (~3.2μl roughly equivalent to 0.1 air-to-fuel ratio) during the compression stroke, a strong pre-ignition response could be induced. A total of 18 lubricants were tested thermodynamically and their pre-ignition frequencies measured via analysis of in-cylinder pressure data. It was found that the occurrence of pre-ignition varied greatly depending on the lubricant injected, with pre-ignition frequencies ranging from 10% to 83%. The measured pre-ignition frequency of a lubricant was found to be inversely proportional to the lubricant density, with a coefficient of determination of 0.91. Calcium and magnesium based detergents were not found to have any measureable effect on pre-ignition frequency. Equally, anti-oxidant additives (including a novel, high-temperature anti-oxidant) were not found to have any effect on pre-ignition frequency in the concentrations considered. Image sets were captured for the deliberate introduction of two different lubricants. Images were captured via the natural light emission from combustion. It was found that pre-ignition most commonly initiated in the area surrounding the active exhaust valve head and resulted in a deflagration that caused the combustion phasing to be advanced. With 97RON gasoline, pre-ignition was rarely sufficiently advanced for end-gas auto-ignition and knock to occur. Image sets were also captured for the natural introduction of lubricant from the top-land crevice subsequent to high-intensity end-gas knock. High-intensity knock was achieved by fuelling with a low octane gasoline. Droplets of lubricant were observed moving within the main charge and causing pre-ignition in cycles subsequent to the natural release of lubricant. A total of 11 lubricant droplets were observed causing pre-ignition within the main charge. Naturally released lubricant droplets were found to survive within the combustion chamber for multiple cycles and lead to a corresponding "on-off" knocking combustion pattern that has been so widely…

Subjects/Keywords: Knock; Low-speed pre-ignition; Super-knock; Oil; Down-sizing

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APA (6^th Edition):

Dingle, S. F. (2014). Lubricant induced pre-ignition in an optical spark-ignition engine. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/14026 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.824232

Chicago Manual of Style (16^th Edition):

Dingle, Simon Frederick. “Lubricant induced pre-ignition in an optical spark-ignition engine.” 2014. Doctoral Dissertation, Brunel University. Accessed April 15, 2021. http://bura.brunel.ac.uk/handle/2438/14026 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.824232.

MLA Handbook (7^th Edition):

Dingle, Simon Frederick. “Lubricant induced pre-ignition in an optical spark-ignition engine.” 2014. Web. 15 Apr 2021.

Vancouver:

Dingle SF. Lubricant induced pre-ignition in an optical spark-ignition engine. [Internet] [Doctoral dissertation]. Brunel University; 2014. [cited 2021 Apr 15]. Available from: http://bura.brunel.ac.uk/handle/2438/14026 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.824232.

Council of Science Editors:

Dingle SF. Lubricant induced pre-ignition in an optical spark-ignition engine. [Doctoral Dissertation]. Brunel University; 2014. Available from: http://bura.brunel.ac.uk/handle/2438/14026 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.824232

University of Alberta

4. Mashkournia, Masoud. Electromagnetic Variable Valve Timing on a Single Cylinder Engine in HCCI and SI.

Degree: MS, Department of Mechanical Engineering, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/mk61rj26s

► An experimental single cylinder engine with variable valve timing is used to study spark ignition and homogeneous charge compression ignition (HCCI). The discrete wavelet transform,… (more)

Subjects/Keywords: SI; HCCI; Valve Timing; Electromagnetic; Knock

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APA (6^th Edition):

Mashkournia, M. (2012). Electromagnetic Variable Valve Timing on a Single Cylinder Engine in HCCI and SI. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/mk61rj26s

Chicago Manual of Style (16^th Edition):

Mashkournia, Masoud. “Electromagnetic Variable Valve Timing on a Single Cylinder Engine in HCCI and SI.” 2012. Masters Thesis, University of Alberta. Accessed April 15, 2021. https://era.library.ualberta.ca/files/mk61rj26s.

MLA Handbook (7^th Edition):

Mashkournia, Masoud. “Electromagnetic Variable Valve Timing on a Single Cylinder Engine in HCCI and SI.” 2012. Web. 15 Apr 2021.

Vancouver:

Mashkournia M. Electromagnetic Variable Valve Timing on a Single Cylinder Engine in HCCI and SI. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Apr 15]. Available from: https://era.library.ualberta.ca/files/mk61rj26s.

Council of Science Editors:

Mashkournia M. Electromagnetic Variable Valve Timing on a Single Cylinder Engine in HCCI and SI. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/mk61rj26s

Luleå University of Technology

5. Lööv, Sara. Effektiv och kontrollerad materialförsörjning i fordonsindustrin : En fallstudie av Scanias CKD-verksamhet.

Degree: 2013, Luleå University of Technology

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42592

► Due to the increased competition in the automotive industry, companies have been forced to become more flexible and to use resourses and information more… (more)

▼ Due to the increased competition in the automotive industry, companies have been forced to become more flexible and to use resourses and information more effectively. The automotive industry is associated with production lines and it is common to use a modulation system, used to manage deep product ranges. CKD productions handle components and modules, which are delivered to international customers where they are assembled to end products. CKD productions are requiring the same components and modules as the production lines and this leads to many complex transportations of material. The study was conducted at Scania in Södertälje that manufactures buses and trucks. In the current situation, the demand for Scania’s products is low, but the demand is expected to increase in the next economic boom. Therefore, Scania has set a target to increase the production of existing products and to increase the number of product variants with existing capacity to ensure future supplies. In order to achieve these objectives, the internal logistics flows associated with the chassis production will have to fundamentally change. These changes are carried out by the project, “Master Process Logistics”, which means that unpredictable transports are substituted to loop traffic by train. The planned changes will also affect the transportations of materials to CKD production, which mean that new methods for material supply to CKD production must be developed. The aim of this study was to develop a framework for efficient and controlled supply of materials to a CKD production in the automotive industry. The aim has been reached by (1) examining the characteristics of the supply of materials in the automotive industry and (2) examining the characteristics of efficient and controlled material supply. The results are based on observations, interviews, focus groups and a literature review.Effective supply of materials is strongly linked to overall company goals and strategy. Concrete measures to achieve the objectives should be formulated and by performing these measures the efficiency can be enhanced. Efficiency aims to affect variables such as cost, time, flexibility, customer service and environment. Actions to increase efficiency are primarily to perform more with fewer resources, JIT, customer focus, minimizing waste and planning transportation routes to create predetermined material flows. The use of trains, return flows and already existing transport routes can lead to increased fill ratio and by that increased efficiency. Controlled supply of materials includes tracking and tracing, meaning to constantly be aware of the location of the products and to have access to information when an unforeseen event occurs. Controlled supply of materials can be created by introducing control points in the flow, predictable routes and good communication.The framework in this study contributes to create efficient and controlled supply of materials to CKD production. The concrete steps are to use a terminal, increase the number of predetermined…

Subjects/Keywords: Technology; Teknik; Logistik; CKD; Completely knock down

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APA (6^th Edition):

Lööv, S. (2013). Effektiv och kontrollerad materialförsörjning i fordonsindustrin : En fallstudie av Scanias CKD-verksamhet. (Thesis). Luleå University of Technology. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42592

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lööv, Sara. “Effektiv och kontrollerad materialförsörjning i fordonsindustrin : En fallstudie av Scanias CKD-verksamhet.” 2013. Thesis, Luleå University of Technology. Accessed April 15, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42592.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lööv, Sara. “Effektiv och kontrollerad materialförsörjning i fordonsindustrin : En fallstudie av Scanias CKD-verksamhet.” 2013. Web. 15 Apr 2021.

Vancouver:

Lööv S. Effektiv och kontrollerad materialförsörjning i fordonsindustrin : En fallstudie av Scanias CKD-verksamhet. [Internet] [Thesis]. Luleå University of Technology; 2013. [cited 2021 Apr 15]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42592.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lööv S. Effektiv och kontrollerad materialförsörjning i fordonsindustrin : En fallstudie av Scanias CKD-verksamhet. [Thesis]. Luleå University of Technology; 2013. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42592

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

King Abdullah University of Science and Technology

6. mubarak ali, mohammed jaasim. Modeling of Pre-ignition and Super-knock in Spark Ignition Engines.

Degree: Physical Science and Engineering (PSE) Division, 2018, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/628315

► Advanced combustion concepts are required to meet the increasing global energy demand and stringent emission regulations imposed by the governments on automobile manufacturers. Improvement in… (more)

▼ Advanced combustion concepts are required to meet the increasing global energy demand and stringent emission regulations imposed by the governments on automobile manufacturers. Improvement in efficiency and reduction in emissions can be achieved by downsizing the Spark Ignition (SI) engines. The operating range of SI engine is limited by occurrence of knock, pre-ignition and the following super-knock due to boosting of intake pressure, to account for the reduction of power, as a result of downsizing the engine. Super-knock, which represents high momentary pressure accompanied with pressure oscillations, is known to permanently damage the moving component of the engines. Therefore fundamental comprehensive understanding of the mechanism involved in pre-ignition and super-knock are required to design highly efficient spark ignition engines with lower emissions that can meet the increasing government regulations. \nThe thesis focuses on auto-ignition characteristics of endgas and the bulk mixture properties that favor transition of pre-ignition to super-knock. Direct numerical studies indicate that super-knock occurs to due to initiation of premature flame front that transition into detonation. In literature, many sources are reported to trigger pre-ignition. Due to the uncertainty of the information on the sources that trigger pre-ignition, it is extremely difficult to predict and control pre-ignition event in SI engines. Since the information on the source of pre-ignition is not available, the main focus of this work is to understand the physical and chemical mechanisms involved in super-knock, factors that influence super-knock and methods to predict super-knock. \n Pre-ignition was initiated at known locations and crank angle using a hotspot of known size and strength. Different parametric cases were studied and the location and timing of pre-ignition initiation is found to be extremely important in determining the transition of pre-ignition event to super-knock. Pre-ignition increases the temperature of the endgas and the overall bulk mixture, that transitions the pre-ignition flame front to a detonation. The transition of the flame propagation mode from deflagration to detonation was investigated with different type of analysis methods and all results confirmed the transition of pre-ignition flame front to detonation that results in super- knock. Advisors/Committee Members: Im, Hong G. (advisor), Johansson, Bengt (committee member), Sarathy, Mani (committee member), Sun, Shuyu (committee member), Rutland, Christopher (committee member).

Subjects/Keywords: super-knock; pre-ignition; sequential auto-ignition

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APA (6^th Edition):

mubarak ali, m. j. (2018). Modeling of Pre-ignition and Super-knock in Spark Ignition Engines. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/628315

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

mubarak ali, mohammed jaasim. “Modeling of Pre-ignition and Super-knock in Spark Ignition Engines.” 2018. Thesis, King Abdullah University of Science and Technology. Accessed April 15, 2021. http://hdl.handle.net/10754/628315.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

mubarak ali, mohammed jaasim. “Modeling of Pre-ignition and Super-knock in Spark Ignition Engines.” 2018. Web. 15 Apr 2021.

Vancouver:

mubarak ali mj. Modeling of Pre-ignition and Super-knock in Spark Ignition Engines. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2018. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/10754/628315.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

mubarak ali mj. Modeling of Pre-ignition and Super-knock in Spark Ignition Engines. [Thesis]. King Abdullah University of Science and Technology; 2018. Available from: http://hdl.handle.net/10754/628315

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Bayle, Simon. Etude de FOXA1 dans les cellules épithéliales mammaires humaines : FOXA1 study in human mammary epithelial cells.

Degree: Docteur es, Sciences, technologie, santé. Génétique, 2013, Université de Bordeaux Segalen

URL: http://www.theses.fr/2013BOR22119

►

Les cancers du sein sont divisés en sous types définis par leur histologie, leur prolifération et l’expression du récepteur aux œstrogènes ER. Notre étude porte… (more)

▼

Les cancers du sein sont divisés en sous types définis par leur histologie, leur prolifération et l’expression du récepteur aux œstrogènes ER. Notre étude porte sur le gène FOXA1 dans le sous-type luminal caractérisé par des cellules bien différenciées, peu prolifératives et exprimant fortement les protéines FOXA1 et ER. Des études suggèrent que FOXA1 est impliqué dans le développement de la glande mammaire, dans la différenciation et la prolifération des cellules mammaires. Dans ce cadre, mon projet de thèse s’articulait autour de trois points ; développer un Knock-In au niveau du gène FOXA1, identifier le rôle de FOXA1 dans la différenciation mammaire et enfin rechercher le rôle des facteurs ER et FOXA1 dans la résistance à l’hormonothérapie. De nombreux tests d’intégration ciblée ont été réalisés à l’aide de différentes matrices de recombinaison et de nucléases spécifiques, les ZFNs. Aucune intégration ciblée n’a finalement été observée. Nous avons montré qu’en fonction du contexte cellulaire, FOXA1 jouait différents rôles dans la différenciation cellulaire et l’expression de la molécule d’adhérence E-Cadhérine. Ces résultats suggèrent que FOXA1 influence l’agressivité tumorale suivant le contexte cellulaire. Nous avons également identifié une amplification d’ER et de FOXA1 dans les cellules tumorales résistantes à l’hormonothérapie par une étude génomique. Les tests in vitro ont montré que la surexpression de FOXA1 augmenterait bien la résistance au fulvestrant mais la surexpression d’ER aurait l’effet inverse, suggérant l’implication d’autres facteurs. De futures recherches nous permettront d’identifier ces facteurs et de préciser les rôles de FOXA1 et d’ER dans la différenciation luminale, l’agressivité tumorale et dans la réponse cellulaire à l’hormonothérapie

Breast cancers are divided into subtypes defined by their histology, proliferation and expression of estrogen receptor ER. Our study focuses on the FOXA1 gene in the luminal subtype characterized by well-differentiated cells, low proliferative and strongly expressing FOXA1 protein and ER. Studies suggest that FOXA1 is involved in the mammary gland development and in the differentiation and proliferation of mammary cells. In this context, my thesis project was structured around three points, develop a knock-in at the FOXA1 gene, identify the role of FOXA1 in mammary differentiation and finally explore the role of ER and FOXA1 in resistance to hormone therapy. Many targeted integration tests were performed using different matrix of recombination and specific nucleases, the ZFNs. No direct integration was finally observed. We showed that depending on the cell context, FOXA1 played different roles in cell differentiation and expression of E-cadherin, an adhesion molecule. These results suggest that FOXA1 influence tumor aggressiveness depending on the cell context. We also identified amplification of ER and FOXA1 in tumor cell resistant to hormone therapy by a genomic study. Surprisingly, in vitro tests showed that overexpression of FOXA1 increased…

Advisors/Committee Members: Iggo, Richard (thesis director).

Subjects/Keywords: Knock-In; FOXA1; Cancer du Sein; Différenciation; Hormonothérapie; Knock-In; FOXA1; Breast Cancer; Differentiation; Hormonotherapy

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APA (6^th Edition):

Bayle, S. (2013). Etude de FOXA1 dans les cellules épithéliales mammaires humaines : FOXA1 study in human mammary epithelial cells. (Doctoral Dissertation). Université de Bordeaux Segalen. Retrieved from http://www.theses.fr/2013BOR22119

Chicago Manual of Style (16^th Edition):

Bayle, Simon. “Etude de FOXA1 dans les cellules épithéliales mammaires humaines : FOXA1 study in human mammary epithelial cells.” 2013. Doctoral Dissertation, Université de Bordeaux Segalen. Accessed April 15, 2021. http://www.theses.fr/2013BOR22119.

MLA Handbook (7^th Edition):

Bayle, Simon. “Etude de FOXA1 dans les cellules épithéliales mammaires humaines : FOXA1 study in human mammary epithelial cells.” 2013. Web. 15 Apr 2021.

Vancouver:

Bayle S. Etude de FOXA1 dans les cellules épithéliales mammaires humaines : FOXA1 study in human mammary epithelial cells. [Internet] [Doctoral dissertation]. Université de Bordeaux Segalen; 2013. [cited 2021 Apr 15]. Available from: http://www.theses.fr/2013BOR22119.

Council of Science Editors:

Bayle S. Etude de FOXA1 dans les cellules épithéliales mammaires humaines : FOXA1 study in human mammary epithelial cells. [Doctoral Dissertation]. Université de Bordeaux Segalen; 2013. Available from: http://www.theses.fr/2013BOR22119

8. Vedin, Jonas. Stochastic Knock Control for Improved Efficiency.

Degree: Vehicular Systems, 2019, Linköping University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-158635

► Increasing the efficiency and performance of internal combustion engines is always of interest in the automotive industry. One limiting factor to achieve this in… (more)

Subjects/Keywords: Knock control; Stochastic knock control; Knock simulation; Control Engineering; Reglerteknik

…5.3 5.4 5.5 5.2.2 5.2.3 Knock 5.3.1 5.3.2 5.3.3 Probability Error at Knock Event… …Threshold Optimisation . . . . . . . . . . . . . . . . . . . . . . . . . Conventional Knock… …Control . . . . . . . . . . . . . . . . . . . . . . Cumulative Summation Knock Control with… …Conventional Control with Optimal Threshold . . . . . . . . . . . . 5.3.5 Likelihood knock control… …with fast-forward algorithm . . . . . . . . . 5.3.6 Live estimation knock control…

10 more images…

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APA (6^th Edition):

Vedin, J. (2019). Stochastic Knock Control for Improved Efficiency. (Thesis). Linköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-158635

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vedin, Jonas. “Stochastic Knock Control for Improved Efficiency.” 2019. Thesis, Linköping University. Accessed April 15, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-158635.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vedin, Jonas. “Stochastic Knock Control for Improved Efficiency.” 2019. Web. 15 Apr 2021.

Vancouver:

Vedin J. Stochastic Knock Control for Improved Efficiency. [Internet] [Thesis]. Linköping University; 2019. [cited 2021 Apr 15]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-158635.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vedin J. Stochastic Knock Control for Improved Efficiency. [Thesis]. Linköping University; 2019. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-158635

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Johannes Gutenberg Universität Mainz

9. Damrath, Ewa Maria. The characterization of the Atxn2-CAG42-knock-in mouse as a model for Spinocerebellar Ataxia Type 2.

Degree: 2012, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2012/3254/

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Die Ursache der neurodegenerativen Erkrankung Spinozerebelläre Ataxie Typ 2 (SCA2) ist eine expandierte Polyglutamin-Domäne im humanen ATXN2-Gen von normalerweise 22 auf über 31 CAGs. Von… (more)

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Die Ursache der neurodegenerativen Erkrankung Spinozerebelläre Ataxie Typ 2 (SCA2) ist eine expandierte Polyglutamin-Domäne im humanen ATXN2-Gen von normalerweise 22 auf über 31 CAGs. Von der Degeneration sind vorwiegend die zerebellären Purkinje Neuronen betroffen, in denen zunehmend zytoplasmatische Aggregate sichtbar werden. Auch wenn die genaue Funktion von ATXN2 und die zugrunde liegenden molekularen Mechanismen noch immer ungeklärt sind, werden ein toxischer Funktionsgewinn sowie der Verlust der normalen Proteinfunktion als mögliche Ursachen diskutiert.rnUm ein wirklichkeitsgetreues Tiermodell für die SCA2 zu haben, wurde eine knock-in Maus generiert, deren einzelnes CAG im Atxn2-Gen durch 42 CAGs ersetzt wurde. Dieses Mausmodell ist durch eine stabile Vererbung der Expansion charakterisiert. Weiterhin zeigt sie ein verringertes Körpergewicht sowie eine spät beginnende motorische Inkoordination, was dem Krankheitsbild von SCA2 entspricht. rnIm Weiteren konnte gezeigt werden, dass, obwohl die Atxn2 mRNA-Spiegel in Großhirn und Kleinhirn erhöht waren, die Menge an löslichem ATXN2 im Laufe der Zeit abnahm und dies mit einem Auftreten an unlöslichem ATXN2 korrelierte. Dieser im Kleinhirn progressive Prozess resultierte schließlich in zytoplasmatischen Aggregaten innerhalb der Purkinje Neuronen alter Mäuse. Der Verlust an löslichem ATXN2 könnte Effekte erklären, die auf einen partiellen Funktionsverlust von ATXN2 zurückzuführen sind, wobei die Aggregatbildung einen toxischen Funktionsgewinn wiederspiegeln könnte. Neben ATXN2 wurde auch sein Interaktor PABPC1 zunehmend unlöslich. Während dies im Großhirn eine Erhöhung der PABPC1 mRNA- und löslichen Proteinspiegel zur Folge hatte, konnte keine kompensatorische Veränderung seiner mRNA und zudem eine Verminderung an löslichem PABPC1 im Kleinhirn beobachtet werden. Auch PABPC1 wurde in Aggregate sequestriert. Diese Unterschiede zwischen Großhirn und Kleinhirn könnten zu der spezifischen Vulnerabilität des Kleinhirns beitragen.rnUm die Folgen auf mRNA-Prozessierung zu untersuchen, wurde ein Transkriptomprofil im mittleren sowie fortgeschrittenen Alter der Mäuse erstellt. Hierbei war eine erhöhte Expression von Fbxw8 im Kleinhirn alter Mäuse auffällig. Als Komponente eines Ubiquitin-E3-Ligase-Komplexes, hilft FBXW8 in der Degradierung von Zielproteinen und könnte somit die Toxizität des expandieren ATXN2 verringern. rnZur näheren Beschreibung der physiologischen Funktion von ATXN2, konnte in ATXN2-knock-out Mäusen gezeigt werden, dass das Fehlen von ATXN2 zu einer reduzierten globalen Proteinsyntheserate führte und somit eine Rolle als Translationsaktivator möglich erscheint. Kompensatorisch wurde eine erhöhte S6-Phosphorylierung gemessen.

The neurodegenerative disease Spinocerebellar Ataxia Type 2 (SCA2) is caused by an expanded polyglutamine domain in the human ATXN2 gene from usually 22 to more than 31 CAGs. The primary site of degeneration is the cerebellar Purkinje neurons in which cytoplasmic aggregates become visible during late stages of the disease. The exact…

Subjects/Keywords: Polyglutamin; Ataxin-2; knock-in Maus; PABPC1; Aggregate; polyglutamine; Ataxin-2; knock-in mouse; PABPC1; aggregates; Life sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Damrath, E. M. (2012). The characterization of the Atxn2-CAG42-knock-in mouse as a model for Spinocerebellar Ataxia Type 2. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2012/3254/

Chicago Manual of Style (16^th Edition):

Damrath, Ewa Maria. “The characterization of the Atxn2-CAG42-knock-in mouse as a model for Spinocerebellar Ataxia Type 2.” 2012. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed April 15, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2012/3254/.

MLA Handbook (7^th Edition):

Damrath, Ewa Maria. “The characterization of the Atxn2-CAG42-knock-in mouse as a model for Spinocerebellar Ataxia Type 2.” 2012. Web. 15 Apr 2021.

Vancouver:

Damrath EM. The characterization of the Atxn2-CAG42-knock-in mouse as a model for Spinocerebellar Ataxia Type 2. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2012. [cited 2021 Apr 15]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2012/3254/.

Council of Science Editors:

Damrath EM. The characterization of the Atxn2-CAG42-knock-in mouse as a model for Spinocerebellar Ataxia Type 2. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2012. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2012/3254/

Oregon State University

10. McKee, Laird Wesley. The effect of the antiknock fluid content in gasoline on piston ring wear as determined by radioactive tracer.

Degree: MS, Mechanical Engineering, 1956, Oregon State University

URL: http://hdl.handle.net/1957/51743

Subjects/Keywords: Gasoline – Anti-knock and anti-knock mixtures

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McKee, L. W. (1956). The effect of the antiknock fluid content in gasoline on piston ring wear as determined by radioactive tracer. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/51743

Chicago Manual of Style (16^th Edition):

McKee, Laird Wesley. “The effect of the antiknock fluid content in gasoline on piston ring wear as determined by radioactive tracer.” 1956. Masters Thesis, Oregon State University. Accessed April 15, 2021. http://hdl.handle.net/1957/51743.

MLA Handbook (7^th Edition):

McKee, Laird Wesley. “The effect of the antiknock fluid content in gasoline on piston ring wear as determined by radioactive tracer.” 1956. Web. 15 Apr 2021.

Vancouver:

McKee LW. The effect of the antiknock fluid content in gasoline on piston ring wear as determined by radioactive tracer. [Internet] [Masters thesis]. Oregon State University; 1956. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/1957/51743.

Council of Science Editors:

McKee LW. The effect of the antiknock fluid content in gasoline on piston ring wear as determined by radioactive tracer. [Masters Thesis]. Oregon State University; 1956. Available from: http://hdl.handle.net/1957/51743

Oregon State University

11. Mingle, John Glenn. Mixture control testing of a spark-ignition engine with anti-detonant admission.

Degree: MS, Mechanical Engineering, 1949, Oregon State University

URL: http://hdl.handle.net/1957/53052

Subjects/Keywords: Gasoline – Anti-knock and anti-knock mixtures

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mingle, J. G. (1949). Mixture control testing of a spark-ignition engine with anti-detonant admission. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/53052

Chicago Manual of Style (16^th Edition):

Mingle, John Glenn. “Mixture control testing of a spark-ignition engine with anti-detonant admission.” 1949. Masters Thesis, Oregon State University. Accessed April 15, 2021. http://hdl.handle.net/1957/53052.

MLA Handbook (7^th Edition):

Mingle, John Glenn. “Mixture control testing of a spark-ignition engine with anti-detonant admission.” 1949. Web. 15 Apr 2021.

Vancouver:

Mingle JG. Mixture control testing of a spark-ignition engine with anti-detonant admission. [Internet] [Masters thesis]. Oregon State University; 1949. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/1957/53052.

Council of Science Editors:

Mingle JG. Mixture control testing of a spark-ignition engine with anti-detonant admission. [Masters Thesis]. Oregon State University; 1949. Available from: http://hdl.handle.net/1957/53052

Oregon State University

12. Seshadri, Erode Venkataramanan. Performance of a spark-ignition engine with admission of some internal knock-suppressing coolants.

Degree: MS, Mechanical Engineering, 1949, Oregon State University

URL: http://hdl.handle.net/1957/53211

Subjects/Keywords: Gasoline – Anti-knock and anti-knock mixtures

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Seshadri, E. V. (1949). Performance of a spark-ignition engine with admission of some internal knock-suppressing coolants. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/53211

Chicago Manual of Style (16^th Edition):

Seshadri, Erode Venkataramanan. “Performance of a spark-ignition engine with admission of some internal knock-suppressing coolants.” 1949. Masters Thesis, Oregon State University. Accessed April 15, 2021. http://hdl.handle.net/1957/53211.

MLA Handbook (7^th Edition):

Seshadri, Erode Venkataramanan. “Performance of a spark-ignition engine with admission of some internal knock-suppressing coolants.” 1949. Web. 15 Apr 2021.

Vancouver:

Seshadri EV. Performance of a spark-ignition engine with admission of some internal knock-suppressing coolants. [Internet] [Masters thesis]. Oregon State University; 1949. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/1957/53211.

Council of Science Editors:

Seshadri EV. Performance of a spark-ignition engine with admission of some internal knock-suppressing coolants. [Masters Thesis]. Oregon State University; 1949. Available from: http://hdl.handle.net/1957/53211

Université Montpellier II

13. Suárez, Catherine. Function and regulation of a serine protease implicated in malaria parasite remodelling and egress : Activité et régulation d'une protéase à serine impliquée dans la maturation et la libération des mérozoïtes de Plasmodium, agent du paludisme.

Degree: Docteur es, Biologie Santé, 2012, Université Montpellier II

URL: http://www.theses.fr/2012MON20209

► Le paludisme demeure une des maladies infectieuses les plus meurtrières au monde. Propagé par la piqûre d’un moustique femelle du genre Anopheles, le parasite du… (more)

▼ Le paludisme demeure une des maladies infectieuses les plus meurtrières au monde. Propagé par la piqûre d’un moustique femelle du genre Anopheles, le parasite du paludisme (Plasmodium) migre dans la circulation sanguine et infecte les cellules hépatiques de la victime. Dans le foie, le parasite se différencie et se reproduit par schizogonie à l’intérieur d’une vacuole parasitophore pour compléter la production de plusieurs milliers de mérozoïtes par cellule hépatique infectée. Ces mérozoïtes sont par la suite libérés dans la circulation sanguine où ils infectent les érythrocytes circulants dans lesquels le parasite subit des cycles d’infection, réplication et libération (processus de sortie actif provoqué par le parasite). Ces cycles répétitifs dans le sang sont à l’origine des symptômes cliniques de la maladie.Des études sur Plasmodium falciparum ont montré que P. falciparum SUB1 (PfSUB1), une protéase à sérine de la famille des subtilisines, est relâchée à l’intérieur de la vacuole parasitophore peu avant la libération des mérozoïtes des hématies. A l’intérieur de cette vacuole, la protéase intervient dans la maturation de protéines membres de la famille des SERA (famille de protéines du type papaïne) ainsi qu’un certain nombre de protéines de surface du mérozoïte (famille des MSP). Un grand intérêt a été porté sur cette protéase, car l’inhibition pharmacologique de l’activité de PfSUB1 bloque le processus de sortie et d’invasion des mérozoïtes dans le stade érythrocytaire du parasite in vitro.Le stade hépatique du parasite est une cible idéale pour le développement de traitements prophylactiques antipaludiques, car il précède la phase symptomatique de la maladie. En conséquence, il est important de mieux comprendre les mécanismes de fonctionnement du parasite à ce stade. Le présent projet avait pour but principal d’effectuer une étude du rôle de SUB1 dans le stade hépatique de Plasmodium. Pour ce faire, ce travail s’est effectué sur l’orthologue de PfSUB1 chez le parasite murin P. berghei. Dans un premier temps, l’expression de PbSUB1 dans les stades hépatiques du parasite a été confirmée en utilisant des anticorps spécifiques et en générant une lignée mutante de P. berghei exprimant la protéine endogène fusionnée à un marqueur hémagglutinine. Par la suite, l’enzyme a été exprimée sous forme recombinante et sa fonction et spécificité ont été partiellement caractérisées. Ce travail confirma que la protéase est capable de cliver des peptides basés sur les séquences de PbMSP1 et PbSERA3, substrats potentiels exprimés dans les stades hépatiques du parasite. Finalement, afin de mieux caractériser la fonction de PbSUB1, deux approches récentes permettant d’effectuer un knock-out conditionnel chez P. berghei ont été testées: le système Tet et la mutagenèse conditionnelle Flp/FRT. Afin d’utiliser cette dernière approche, une nouvelle méthode pour insérer des sites de reconnaissances Flp (sites FRT) dans les régions intergéniques de clones (dans ce cas, un clone comprenant pbsub1 et gènes voisins) provenant d’une… Advisors/Committee Members: Blackman, Michael J. (thesis director).

Subjects/Keywords: Paludisme; Plasmodium berghei; Sub1; Stade hépatique; Knock-out génétique; Malaria; Plasmodium berghei; Sub1; Liver stage; Gene knock-out

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suárez, C. (2012). Function and regulation of a serine protease implicated in malaria parasite remodelling and egress : Activité et régulation d'une protéase à serine impliquée dans la maturation et la libération des mérozoïtes de Plasmodium, agent du paludisme. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2012MON20209

Chicago Manual of Style (16^th Edition):

Suárez, Catherine. “Function and regulation of a serine protease implicated in malaria parasite remodelling and egress : Activité et régulation d'une protéase à serine impliquée dans la maturation et la libération des mérozoïtes de Plasmodium, agent du paludisme.” 2012. Doctoral Dissertation, Université Montpellier II. Accessed April 15, 2021. http://www.theses.fr/2012MON20209.

MLA Handbook (7^th Edition):

Suárez, Catherine. “Function and regulation of a serine protease implicated in malaria parasite remodelling and egress : Activité et régulation d'une protéase à serine impliquée dans la maturation et la libération des mérozoïtes de Plasmodium, agent du paludisme.” 2012. Web. 15 Apr 2021.

Vancouver:

Suárez C. Function and regulation of a serine protease implicated in malaria parasite remodelling and egress : Activité et régulation d'une protéase à serine impliquée dans la maturation et la libération des mérozoïtes de Plasmodium, agent du paludisme. [Internet] [Doctoral dissertation]. Université Montpellier II; 2012. [cited 2021 Apr 15]. Available from: http://www.theses.fr/2012MON20209.

Council of Science Editors:

Suárez C. Function and regulation of a serine protease implicated in malaria parasite remodelling and egress : Activité et régulation d'une protéase à serine impliquée dans la maturation et la libération des mérozoïtes de Plasmodium, agent du paludisme. [Doctoral Dissertation]. Université Montpellier II; 2012. Available from: http://www.theses.fr/2012MON20209

14. Mouchacca, Pierre. Granzyme B-td TOMATO, un nouvel outil fluorescent pour le suivi de la cytolyse chez la souris : Role of non-coding regulatory RNAs on the adaptative response of speudomonas brassicacearum to the rhizosphere and changing environments.

Degree: Docteur es, Immunologie, 2012, Aix Marseille Université

URL: http://www.theses.fr/2012AIXM4008

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La fonction de cytolyse est un mécanisme majeur des effecteurs du système immunitaire pour éliminer les cellules infectées ou tumorales. Cette fonction associe l'activité de… (more)

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La fonction de cytolyse est un mécanisme majeur des effecteurs du système immunitaire pour éliminer les cellules infectées ou tumorales. Cette fonction associe l'activité de la perforine, qui forme des pores dans la membrane d'une cellule cible, à la sécrétion de protéases: les granzymes. Ces dernières sont des molécules pro-apoptotiques qui induisent la mort de la cellule cible. Les granzymes et en particulier granzyme B ciblent plusieurs voies intracellulaires complémentaires pour assurer l'efficacité de la cytolyse. Or il est difficile d'observer directement la fonction de cytolyse au cours de réponse immunitaire in vivo dans des conditions physiologiques. Dans les travaux présentés dans cette thèse, nous avons développé un nouveau modèle qui permet de suivre la fonction de cytolyse en temps réel par l'expression d'une protéine de fusion fluorescente GZMB-tdTomato. Les résultats obtenus par expression rétrovirale ont montré que la protéine de fusion est correctement exprimée dans les vésicules cytolytiques qui deviennent fluorescentes. Dans un second temps, nous avons réalisé un nouveau modèle murin qui exprime GZMB-tdTomato de manière substituée au GZMB natif par recombinaison homologue (Knock In). Nous avons mis en évidence que la protéine de fusion conserve l'activité catalytique de la protéine native et ses caractéristiques (conditions d'expression, de maturation, de sécrétion et demeure active après le passage dans la cellule cible lors de la cytolyse). En utilisant un modèle murin exprimant un TCR transgénique nous avons pu suivre le déroulement de la fonction de cytolyse de lymphocytes cytotoxiques en temps réel par video microscopies.

Cytolysis is a major function used by the immune system's effectors to kill infected or tumor cells. Cytolysis depends on the pore forming protein perforin and the secretion of proteases of the granzyme family. Granzymes, including granzyme B (GZMB) have pro-apoptotic features and induce target cell death. Several complementary pathways are triggered by granzymes to ensure efficient cytolysis. It remains difficult to directly observe cytolysis during in vivo immune responses under physiological conditions. In this PhD we developed a new model to visualize cytolytic function in real time by expression of a fusion protein: GZMB-tdTomato. Results obtained from retroviral transduction showed that the fusion protein is correctly expressed in cytolytic vesicles, which became fluorescent. We then constructed a new mouse model by homologous recombination (Knock In) that express GZMB-tdTomato substituted for the native GZMB. The fusion protein conserves the catalytic activity of GZMB and its features (expression, maturation, secretion conditions) and remains active after its passage into target cells. Using TCR transgenic OTI cells, we followed the sequence of events of cytolysis from lymphocytes in real time by videomicroscopy. We also observed the cytolytic vesicles relocalization towards the cell contact zone and the death of target cell by cytolysis. Finally, we studied in vivo…

Advisors/Committee Members: Boyer, Claude (thesis director).

Subjects/Keywords: Cytolyse; Granzyme B; Souris; Knock In; Microscopie confocale; Ctl; Cytolysis; Granzyme B, mouse, Knock In, confocal microscopy; Ctl

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mouchacca, P. (2012). Granzyme B-td TOMATO, un nouvel outil fluorescent pour le suivi de la cytolyse chez la souris : Role of non-coding regulatory RNAs on the adaptative response of speudomonas brassicacearum to the rhizosphere and changing environments. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2012AIXM4008

Chicago Manual of Style (16^th Edition):

Mouchacca, Pierre. “Granzyme B-td TOMATO, un nouvel outil fluorescent pour le suivi de la cytolyse chez la souris : Role of non-coding regulatory RNAs on the adaptative response of speudomonas brassicacearum to the rhizosphere and changing environments.” 2012. Doctoral Dissertation, Aix Marseille Université. Accessed April 15, 2021. http://www.theses.fr/2012AIXM4008.

MLA Handbook (7^th Edition):

Mouchacca, Pierre. “Granzyme B-td TOMATO, un nouvel outil fluorescent pour le suivi de la cytolyse chez la souris : Role of non-coding regulatory RNAs on the adaptative response of speudomonas brassicacearum to the rhizosphere and changing environments.” 2012. Web. 15 Apr 2021.

Vancouver:

Mouchacca P. Granzyme B-td TOMATO, un nouvel outil fluorescent pour le suivi de la cytolyse chez la souris : Role of non-coding regulatory RNAs on the adaptative response of speudomonas brassicacearum to the rhizosphere and changing environments. [Internet] [Doctoral dissertation]. Aix Marseille Université 2012. [cited 2021 Apr 15]. Available from: http://www.theses.fr/2012AIXM4008.

Council of Science Editors:

Mouchacca P. Granzyme B-td TOMATO, un nouvel outil fluorescent pour le suivi de la cytolyse chez la souris : Role of non-coding regulatory RNAs on the adaptative response of speudomonas brassicacearum to the rhizosphere and changing environments. [Doctoral Dissertation]. Aix Marseille Université 2012. Available from: http://www.theses.fr/2012AIXM4008

15. Helary, Louise. Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères : In vivo validation of the implication of new genes in mammalian muscle development.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2019, Limoges

URL: http://www.theses.fr/2019LIMO0053

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Même si les acteurs majeurs du développement musculaire ont été identifiés et les voies de transductions décrites, d’autres régulateurs restent encore à découvrir. Un crible… (more)

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Même si les acteurs majeurs du développement musculaire ont été identifiés et les voies de transductions décrites, d’autres régulateurs restent encore à découvrir. Un crible ARNi pratiqué sur un modèle cellulaire couramment utilisé, la lignée myoblastique C2C12, a identifié 20 nouveaux gènes potentiellement impliqués dans la myogenèse in vitro. Au cours de ma thèse, deux de ces gènes ont été invalidés sur modèle souris en utilisant la technologie CRISPR/Cas9 pour valider in vivo leur implication. Pour l’un d’entre eux, seuls les animaux hétérozygotes ont pu être étudiés puisqu’une létalité précoce a été observée chez les homozygotes mutés. Aucune anomalie du développement musculaire n’a été mise en évidence. Une étude plus fine dans les premières phases du développement embryonnaire nous a permis de montrer le rôle indispensable de cette protéine précocement. L’étude du second gène – dont les analyses se poursuivent – semble confirmer in vivo le rôle de ce gène au cours de la myogenèse. Pour éviter la survenue de létalité embryonnaire et observer rapidement les effets de l’invalidation d’autres gènes, une technique de transgenèse somatique s’appuyant sur l’ARN interférence a été mis en place via l’injection de lentivirus contenant une cassette d’expression de shRNA directement dans le tibialis antérieur des souris. La validation de cette approche a été faite sur le gène de la myostatine, régulateur négatif du développement musculaire, et a montré une diminution de l’expression du gène associée à une augmentation de l’aire des fibres musculaires. La même approche appliquée à trois autres gènes renforce l’hypothèse de l’implication d’un des gènes dans le développement musculaire. Cette approche permet donc un crible rapide « in vivo » de gènes identifiés in vitro. Cependant, certaines améliorations doivent être apportées au protocole au regard des résultats obtenus.

Even if the major actors and transduction pathways of muscle development have been identified, there are still unknown regulatory factors. An in vitro RNAi screening performed on C2C12 myoblastic cells has permitted to identify 20 novel genes potentially implicated in myogenesis. During my thesis, two of these genes were invalidated on mouse model using CRISPR/Cas9 technology in order to confirm their implication in vivo. For the first gene, due to an early lethality occurring in homozygous mutated animals, only heterozygous animals were studied and there was no muscular development anomaly detected. A refined study of earlier stages of embryonic development permitted to show the essential role of the protein in these phases. The study of the second gene, still in progress, seems to confirm in vivo the implication of the gene on the myogenesis. In order to avoid embryonic lethality due to germline invalidation and to observe more rapidly the effects of gene invalidation in muscle, we developed a technique of somatic transgenesis based on RNA interference. Lentivirus containing a shRNA expression cassette was injected directly into the tibialis anterior…

Advisors/Committee Members: Véronique, Blanquet (thesis director), Duchesne-Collardot, Amandine (thesis director).

Subjects/Keywords: Développement musculaire; Knock-out; ShRNA; CRISPR/Cas9; DNAJC2; Muscle development; Knock-out; ShRNA; CRISPR/Cas9; DNAJC2; 599

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Helary, L. (2019). Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères : In vivo validation of the implication of new genes in mammalian muscle development. (Doctoral Dissertation). Limoges. Retrieved from http://www.theses.fr/2019LIMO0053

Chicago Manual of Style (16^th Edition):

Helary, Louise. “Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères : In vivo validation of the implication of new genes in mammalian muscle development.” 2019. Doctoral Dissertation, Limoges. Accessed April 15, 2021. http://www.theses.fr/2019LIMO0053.

MLA Handbook (7^th Edition):

Helary, Louise. “Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères : In vivo validation of the implication of new genes in mammalian muscle development.” 2019. Web. 15 Apr 2021.

Vancouver:

Helary L. Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères : In vivo validation of the implication of new genes in mammalian muscle development. [Internet] [Doctoral dissertation]. Limoges; 2019. [cited 2021 Apr 15]. Available from: http://www.theses.fr/2019LIMO0053.

Council of Science Editors:

Helary L. Validation in vivo de l'implication de nouveaux gènes impliqués dans le développement musculaire des mammifères : In vivo validation of the implication of new genes in mammalian muscle development. [Doctoral Dissertation]. Limoges; 2019. Available from: http://www.theses.fr/2019LIMO0053

16. De Santis, Flavia. Genome editing to understand neural circuits formation : a novel CRISPR/Cas9-based strategy for conditional mutagenesis and functional study of the role of the meteorin gene family in zebrafish neurodevelopment : Edition du génome pour comprendre la formation des circuits neuronaux : une nouvelle stratégie CRSPR/Cas9 pour la mutagenèse conditionnelle et étude fonctionnelle du rôle de la famille des gènes des météorines dans le développement neurologique du poisson zébra.

Degree: Docteur es, Neuroscience, 2017, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2017PA066269

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Depuis quelques années, le poisson zèbre (Danio rerio) est devenu un modèle de choix pour l'étude du système nerveux et de ses fonctions. Récemment, des… (more)

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Depuis quelques années, le poisson zèbre (Danio rerio) est devenu un modèle de choix pour l'étude du système nerveux et de ses fonctions. Récemment, des technologies nouvelles d'édition du génome permettent la génération d'allèles mutés de manière constitutionnelle et l'étude fonctionnelle de gènes chez ce modèle vertébré. Néanmoins, certains loci nécessite une inactivation spatiotemporelle précise et contrôlée. La première partie de ma thèse décrit la mise au point d'une nouvelle stratégie de disruption génétique de manière tissu-spécifique, basée sur la technologie du CRISPR/Cas9 et du système UAS/Gal4. Cette technique permet l'introduction de mutations somatiques dans des tissus, des clones ou des cellules individuelles préalablement génétiquement marqués, rendant ainsi possible le suivi in vivo de l'effet de la mutation générée grâce au gène rapporteur. La seconde partie de ma thèse se centre sur l'étude fonctionnelle d'une famille des gènes, les meteorines, durant le développement du système nerveux et lors du ciblage axonale chez le poisson zèbre. Les Meteorines sont des protéines conservées chez les vertébrés qui ont été impliquées dans la prolifération, la différentiation des progéniteurs de neurones et notamment dans l'élongation axonale in vitro. Nous avons pu mettre en évidence que les meteorines sont exprimées le long de la ligne médiane du système nerveux chez les larves et au niveau du plancher de la partie postérieure du cerveau et de la moelle épinière. Par l'utilisation du CRISPR/Cas9, nous avons généré des lignées mutantes pour chaque gène meteorine et avons ainsi procédé à l'analyse de l'établissement des projections axonales dans ces lignées mutantes.

In recent years, the zebrafish (Danio rerio) has emerged as a powerful model organism to study neuronal circuit development and function. To date, different genome editing technologies allow the generation of constitutive mutant alleles, permitting the study of gene loss-of-function in this vertebrate model. Nevertheless, to assess the role of certain loci it might be required a precise spatiotemporal control of gene inactivation. The rst part of my thesis describes a novel strategy for tissue-specific gene disruption based on the CRISPR/Cas9 and the Gal4/UAS systems. The described technique allows the induction of somatic mutations in genetically labeled tissues, cell clones or single cells, making it possible to follow the effect of gene disruption in vivo via reporter gene expression. The second part of the thesis focuses on the functional analysis of the role of the meteorin gene family during neuronal development and axonal targeting in zebra sh. Meteorin family is conserved among vertebrates and its members have been shown to be involved in neuronal progenitor proliferation and differentiation and axonal elongation, in vitro. We used the zebrafish nervous system as a model to dissect the role of Meteorins during embryonic development, focusing on their potential role as novel guidance molecules. Interestingly, we found that genes belonging…

Advisors/Committee Members: Del Bene, Filippo (thesis director).

Subjects/Keywords: CRISPR/Cas9; Knock-Out conditionnel; Analyse clonale; Meteorin; Guidage axonal; Poisson zèbre; Tissue-speific knock-out; Meteorin; Zebrafish; 612.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

De Santis, F. (2017). Genome editing to understand neural circuits formation : a novel CRISPR/Cas9-based strategy for conditional mutagenesis and functional study of the role of the meteorin gene family in zebrafish neurodevelopment : Edition du génome pour comprendre la formation des circuits neuronaux : une nouvelle stratégie CRSPR/Cas9 pour la mutagenèse conditionnelle et étude fonctionnelle du rôle de la famille des gènes des météorines dans le développement neurologique du poisson zébra. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2017PA066269

Chicago Manual of Style (16^th Edition):

De Santis, Flavia. “Genome editing to understand neural circuits formation : a novel CRISPR/Cas9-based strategy for conditional mutagenesis and functional study of the role of the meteorin gene family in zebrafish neurodevelopment : Edition du génome pour comprendre la formation des circuits neuronaux : une nouvelle stratégie CRSPR/Cas9 pour la mutagenèse conditionnelle et étude fonctionnelle du rôle de la famille des gènes des météorines dans le développement neurologique du poisson zébra.” 2017. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed April 15, 2021. http://www.theses.fr/2017PA066269.

MLA Handbook (7^th Edition):

De Santis, Flavia. “Genome editing to understand neural circuits formation : a novel CRISPR/Cas9-based strategy for conditional mutagenesis and functional study of the role of the meteorin gene family in zebrafish neurodevelopment : Edition du génome pour comprendre la formation des circuits neuronaux : une nouvelle stratégie CRSPR/Cas9 pour la mutagenèse conditionnelle et étude fonctionnelle du rôle de la famille des gènes des météorines dans le développement neurologique du poisson zébra.” 2017. Web. 15 Apr 2021.

Vancouver:

De Santis F. Genome editing to understand neural circuits formation : a novel CRISPR/Cas9-based strategy for conditional mutagenesis and functional study of the role of the meteorin gene family in zebrafish neurodevelopment : Edition du génome pour comprendre la formation des circuits neuronaux : une nouvelle stratégie CRSPR/Cas9 pour la mutagenèse conditionnelle et étude fonctionnelle du rôle de la famille des gènes des météorines dans le développement neurologique du poisson zébra. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2017. [cited 2021 Apr 15]. Available from: http://www.theses.fr/2017PA066269.

Council of Science Editors:

De Santis F. Genome editing to understand neural circuits formation : a novel CRISPR/Cas9-based strategy for conditional mutagenesis and functional study of the role of the meteorin gene family in zebrafish neurodevelopment : Edition du génome pour comprendre la formation des circuits neuronaux : une nouvelle stratégie CRSPR/Cas9 pour la mutagenèse conditionnelle et étude fonctionnelle du rôle de la famille des gènes des météorines dans le développement neurologique du poisson zébra. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2017. Available from: http://www.theses.fr/2017PA066269

Stellenbosch University

17. Leigh, David Ken. Characterisation of synergestic and antagonistic petrol component chemical class effects on octane blending behaviour.

Degree: MEng, Mechanical and Mechatronic Engineering, 2020, Stellenbosch University

URL: http://hdl.handle.net/10019.1/108323

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ENGLISH ABSTRACT: Knock free operation has influenced the development of internal combustion (IC) engine technology and spark ignition (SI) fuels. Knock is the noise associated… (more)

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ENGLISH ABSTRACT: Knock free operation has influenced the development of internal combustion (IC) engine technology and spark ignition (SI) fuels. Knock is the noise associated with the harmful and abnormal combustion phenomenon known as autoignition. For a given engine configuration, the propensity of knock in IC engines depends primarily on the anti-knock quality of the fuel. Research octane number (RON) and motor octane number (MON) are measured fuel properties indicating a fuel s resistance to autoignition. Fuel producers ultimately seek to produce fuels with RON and MON values suitable for modern IC engines, however, the highly complex non-linear blending interactions between the constituents of the fuel recipe make this challenging. The present research was undertaken to characterise the non-linear synergistic and antagonistic octane blending behaviours between important common chemical class components, carefully selected to represent the SI fuel recipe on a fundamental level. The RON and MON values for all binary combinations of these components at various blend ratios were measured using Stellenbosch University’s uniquely modified Cooperative Fuels Research engine. All octane experiments were performed in accordance with the American Society for Testing and Materials D2699 and D2700 standards. The measured RON and MON data showed results consistent with the literature and highlighted a shortage of research data for some binary blends. The project hypothesis was supported, showing that non-linearities do exist in octane blending between binary combinations and that more than one non-linearity can exist in a binary blend. Using this and the measured data, conclusions were drawn on the links between the various chemical classes, blending ratios and the non-linearities in octane number. It was demonstrated that octane sensitivity is a function of blend ratio and provides insight into improving fuel recipes for modern SI engines. The octane boosting capabilities of a non-metallic octane booster, was shown to significantly boost the octane of some of the selected components and had no significant effect on others. It was found to influence the RON value more than the MON value of a blend. The suitability of two well established empirical octane prediction models were investigated for use in predicting octane. The predicted data was compared to the measured data and deficiency in the better performing model was identified and then optimised to improve the octane predictions for the binary blends.

AFRIKAANSE OPSOMMING: Die ontwikkeling van binnebrand motor-tegnologie en vonkontsteking (VO) brandstowwe word beïnvloed deur die vereiste vir klopvrye werking van die enjin. Klop is die geraas verwant aan die skadelike en abnormale verbrandingsverskynsel wat bekend staan as outo-ontsteking. Die neiging tot klop vir ‘n gegewe binnebrand motor konfigurasie, is hoofsaaklik afhanklik van die anti-klop kwaliteit van die brandstof. Die navorsingsoktaansyfer (NOS) en mo- toroktaansyfer (MOS) is meetbare brandstof…

Advisors/Committee Members: Haines, R., Floweday, G., Stellenbosch University. Faculty of Engineering. Dept. of Mechanical and Mechatronic Engineering..

Subjects/Keywords: Synergestics; Chemical inhibitors; Petroleum – Anti-knock and anti-knock mixtures; Petroleum – Analytics; Internal combustion engines – Fuel systems; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leigh, D. K. (2020). Characterisation of synergestic and antagonistic petrol component chemical class effects on octane blending behaviour. (Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/108323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leigh, David Ken. “Characterisation of synergestic and antagonistic petrol component chemical class effects on octane blending behaviour.” 2020. Thesis, Stellenbosch University. Accessed April 15, 2021. http://hdl.handle.net/10019.1/108323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leigh, David Ken. “Characterisation of synergestic and antagonistic petrol component chemical class effects on octane blending behaviour.” 2020. Web. 15 Apr 2021.

Vancouver:

Leigh DK. Characterisation of synergestic and antagonistic petrol component chemical class effects on octane blending behaviour. [Internet] [Thesis]. Stellenbosch University; 2020. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/10019.1/108323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leigh DK. Characterisation of synergestic and antagonistic petrol component chemical class effects on octane blending behaviour. [Thesis]. Stellenbosch University; 2020. Available from: http://hdl.handle.net/10019.1/108323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Vasiljevska Milijana. Karakteristike akutne zapaljenske reakcije miševa sa uklonjenim genom za makrofagni inhibitorni faktor.

Degree: 2016, University of Novi Sad

URL: https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija14618394333797.pdf?controlNumber=(BISIS)100834&fileName=14618394333797.pdf&id=5504&source=OATD&language=en ; https://www.cris.uns.ac.rs/record.jsf?recordId=100834&source=OATD&language=en

► Cilj ovog rada je bio da se ispitaju karakteristike sterilne zapaljenske reakcije u miševa sa delecijom Mif gena, korišćenjem modela subkutane implantacije polivinilskih sunđera.… (more)

▼ Cilj ovog rada je bio da se ispitaju karakteristike sterilne zapaljenske reakcije u miševa sa delecijom Mif gena, korišćenjem modela subkutane implantacije polivinilskih sunđera. Iako je MIF poznat pro-inflamacijski citokin o njegovom uticaju na procese regeneracije nakon oštećenja tkiva postoje kontradiktorni podaci. Zbog toga su u pokušaju razumevanja ovog problema ispitivani dinamika promena ćelijskih elemenata u eksudatu rane, procesi apoptoze inflamacijskih ćelija i njihova funkcionalna sposobnost in vitro (adhezivnost i produkcija NO). Ovi parametri su povezivani sa nivoom različitih biomolekula u eksudatu (pro- i anti-inflamacijskih citokina), hemokina; faktora rasta reaktanata akutne faze i NO). Dobijeni rezultati su poređeni sa rezultatima kod kontrolnog divljeg soja C57BL/6 (Mif+/+) miševa sa očuvanim Mif genom. Pokazano je da ranu fazu inflamacije (24 časa posle implantacije sunđera) karakteriše smanjeno nakupljanje neutrofilnih granulocita, manja produkcija IL-β, IFN-γ, IL-12 i NO, povećana produkcija IL-33 i GM-CSF i neočekivano manja apoptoza neutrofila u odnosu na divlji soj miševa. Shodno očekivanju, inflamacijske ćelije u kulturi su produkovale više NO, imale veću spontanu apoptozu koja se bitnije nije menjala pod uticajem LPS-a, ali su takođe neočekivano imale povećanu adhezivnost za plastiku. Kasnu fazu inflamacije (10. dana od implantacije sunđera) je karakterisalo manje nakupljanje granulocita; makrofaga i dendritskih ćelija što nije bilo povezano sa brojem neutrofila i monocita u perifernoj krvi, veće stvaranje džinovskih multijedarnih makrofaga i povećanje apoptoze kako neutrofila tako i makrofaga. U eksudatu su nađeni niži nivoi NO, IL-β, TNF-α i CXCL10 a povećani nivoi IL-12, IL- 33, IL-1Ra i CRP. Inflamacijske ćelije su u kulturi produkovale niže nivoe NO ali su, slično kao u ranoj fazi, imale povećanu spontanu i indukovanu adhezivnost za plastiku, u odnosu na divlji soj miševa. Na osnovu dobijenih rezultata se može zaključiti da predominacija anti-inflamacijskih medijatora kod Mif-/- miševa, praćena smanjenom mobilizacijom inflamacijskih ćelija u povređeno tkivo i povećanjem njihove apoptoze, mogu delovati povoljno na bržu rezoluciju inflamacije. Advisors/Committee Members: Belić Branislava, Cincović Marko R., Čolić Miodrag.

Subjects/Keywords: Makrofagni inhibitorni faktor,"Knock-out" životinje,Inflamacija,Apoptoza,Citokini,NO; Macrophage inhibitory factor"Knock-out" animals,Inflammation,Apoptosis,Cytokines,NО

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Milijana, V. (2016). Karakteristike akutne zapaljenske reakcije miševa sa uklonjenim genom za makrofagni inhibitorni faktor. (Thesis). University of Novi Sad. Retrieved from https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija14618394333797.pdf?controlNumber=(BISIS)100834&fileName=14618394333797.pdf&id=5504&source=OATD&language=en ; https://www.cris.uns.ac.rs/record.jsf?recordId=100834&source=OATD&language=en

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Milijana, Vasiljevska. “Karakteristike akutne zapaljenske reakcije miševa sa uklonjenim genom za makrofagni inhibitorni faktor.” 2016. Thesis, University of Novi Sad. Accessed April 15, 2021. https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija14618394333797.pdf?controlNumber=(BISIS)100834&fileName=14618394333797.pdf&id=5504&source=OATD&language=en ; https://www.cris.uns.ac.rs/record.jsf?recordId=100834&source=OATD&language=en.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Milijana, Vasiljevska. “Karakteristike akutne zapaljenske reakcije miševa sa uklonjenim genom za makrofagni inhibitorni faktor.” 2016. Web. 15 Apr 2021.

Vancouver:

Milijana V. Karakteristike akutne zapaljenske reakcije miševa sa uklonjenim genom za makrofagni inhibitorni faktor. [Internet] [Thesis]. University of Novi Sad; 2016. [cited 2021 Apr 15]. Available from: https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija14618394333797.pdf?controlNumber=(BISIS)100834&fileName=14618394333797.pdf&id=5504&source=OATD&language=en ; https://www.cris.uns.ac.rs/record.jsf?recordId=100834&source=OATD&language=en.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Milijana V. Karakteristike akutne zapaljenske reakcije miševa sa uklonjenim genom za makrofagni inhibitorni faktor. [Thesis]. University of Novi Sad; 2016. Available from: https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija14618394333797.pdf?controlNumber=(BISIS)100834&fileName=14618394333797.pdf&id=5504&source=OATD&language=en ; https://www.cris.uns.ac.rs/record.jsf?recordId=100834&source=OATD&language=en

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Isabella Monteiro Guimaraes. Participação do receptor metabotrópico de glutamato 5 na fisiologia de comportamentos motores e nas alterações motoras que ocorrem em camundongos modelo da doença de Huntington.

Degree: 2014, Universidade Federal de Minas Gerais; UFMG

URL: http://hdl.handle.net/1843/BUOS-9PFHS2

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Exportado OPUS

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Introdução: A… (more)

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Introdução: A doença de Huntington (DH) é uma desordem neurodegenerativa, progressiva e hereditária, que evolui inevitavelmente à morte. Uma vez que vários estudos indicam que o receptor metabotrópico de glutamato 5 (mGluR5) pode ter um papel importante na DH e na modulação de movimentos hipercinéticos, decidimos investigar se o mGluR5 tem um papel na hipercinesia envolvida na DH. Resultados: Os resultados obtidos em nosso estudo demonstraram que o mGluR5 interfere na atividade locomotora através de substratos neurais específicos. Para determinar o papel mGluR5 em DH, foi realizado cruzamentos entre camundongos modelo para a DH (HdhQ111/Q111) e seu controle (HdhQ20/Q20) e camundongos knockout para mGluR5 (mGluR5-/-). Foi observado que a deleção do mGluR5 altera atividade locomotora dos camundongos modelo da DH, indicando que existe uma interação funcional entre a Htt mutante e o mGluR5. Ademais, foi caracterizada uma interação funcional da Htt mutante com o mGluR5 capaz de alterar a atividade locomotora de camundongos. Para investigar o mecanismo subjacente a estas alterações de locomoção, foi realizado um ensaio de microarranjo. A expressão de um número de genes envolvidos na regulação do movimento, tal como a dineína cadeia leve, cadeia pesada e dinactina, foram alteradas em camundongos modelo para DH desprovidos da expressão de mGluR5. Tais alterações de expressão foram confirmadas por qPCR. Conclusão: Os resultados indicam que o mGluR5 pode desempenhar um papel no controle do movimento envolvendo vários substratos neurais, podendo estar envolvido na hipercinesia observada na DH. Além disso, resultados do ensaio de microarranjo sugerem que as alterações motoras promovidas pela deleção gênica do mGluR5 no camundongo modelo da DH podem ocorrer devido à alteração da expressão de genes que codificam proteínas relacionadas a motricidade.

Introduction: Huntington's disease (HD) is a neurodegenerative disorder, progressive and hereditary, that evolves inevitably to death. As we and others have demonstrated that the metabotropic glutamate receptor 5 (mGluR5) may have a role in HD pathology and that mGluR5 is involved in hyperkinetic movements, we decided to investigate whether mGluR5 has a role in HD-related hyperkinesia. Results: The results obtained in our study demonstrate that mGluR5 stimulation interferes in locomotor activity by acting on specific neural substrates. To determine mGluR5 role in HD, we have crossed HdhQ20/Q20and HdhQ111/Q111 mice with mGluR5 knockout mice (mGluR5-/-). mGluR5 knockout alters HD mice locomotor activity, indicating that there is a functional interaction between mutant Htt and mGluR5. To investigate the underlying changes in this locomotion regulation, a microarray assay was performed. The expression of a number of genes involved in movement…

Advisors/Committee Members: Fabiola Mara Ribeiro, Grace Schenatto Pereira.

Subjects/Keywords: Modelo Knock-in; Doença de Huntington; mGluR5; Locomoção; Huntington, Doença de; Neurociências; Modelo Knock-in; Receptores de glutamato metabotrópico

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guimaraes, I. M. (2014). Participação do receptor metabotrópico de glutamato 5 na fisiologia de comportamentos motores e nas alterações motoras que ocorrem em camundongos modelo da doença de Huntington. (Masters Thesis). Universidade Federal de Minas Gerais; UFMG. Retrieved from http://hdl.handle.net/1843/BUOS-9PFHS2

Chicago Manual of Style (16^th Edition):

Guimaraes, Isabella Monteiro. “Participação do receptor metabotrópico de glutamato 5 na fisiologia de comportamentos motores e nas alterações motoras que ocorrem em camundongos modelo da doença de Huntington.” 2014. Masters Thesis, Universidade Federal de Minas Gerais; UFMG. Accessed April 15, 2021. http://hdl.handle.net/1843/BUOS-9PFHS2.

MLA Handbook (7^th Edition):

Guimaraes, Isabella Monteiro. “Participação do receptor metabotrópico de glutamato 5 na fisiologia de comportamentos motores e nas alterações motoras que ocorrem em camundongos modelo da doença de Huntington.” 2014. Web. 15 Apr 2021.

Vancouver:

Guimaraes IM. Participação do receptor metabotrópico de glutamato 5 na fisiologia de comportamentos motores e nas alterações motoras que ocorrem em camundongos modelo da doença de Huntington. [Internet] [Masters thesis]. Universidade Federal de Minas Gerais; UFMG; 2014. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/1843/BUOS-9PFHS2.

Council of Science Editors:

Guimaraes IM. Participação do receptor metabotrópico de glutamato 5 na fisiologia de comportamentos motores e nas alterações motoras que ocorrem em camundongos modelo da doença de Huntington. [Masters Thesis]. Universidade Federal de Minas Gerais; UFMG; 2014. Available from: http://hdl.handle.net/1843/BUOS-9PFHS2

20. Rafael Luiz da Silva. Caracterização do sinal do fenômeno de detonação utilizando filtros adaptativos e estimador de potência.

Degree: 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/3/3140/tde-22102014-122132/

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O fenômeno de detonação que ocorre em motores de combustão interna é uma restrição de desempenho para baixas emissões de poluentes, altas solicitações de torque… (more)

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O fenômeno de detonação que ocorre em motores de combustão interna é uma restrição de desempenho para baixas emissões de poluentes, altas solicitações de torque (as quais resultariam em mais potência entregue pelo motor), economia de combustível e também é uma fonte de ruídos desagradáveis ao condutor do veículo. No Brasil, tal fenômeno pode ter ainda uma utilidade benéfica se identificado de forma leal, tendo em vista que a detonação é mais fácil de ocorrer com gasolina do que com álcool. Assim, a ocorrência de detonação pode ser um auxílio no gerenciamento otimizado da injeção, ignição, bem como pode possibilitar a identificação do tipo de combustível. Para isso, técnicas de processamento de sinais podem ser utilizadas para caracterizar tal fenômeno. Este trabalho tem como foco a identificação (ocorrência) e caracterização (intensidade e duração) do sinal de detonação utilizando filtragem adaptativa e estimadores de potência, onde o desempenho individual e combinado destes algoritmos é avaliado. Inicialmente, um modelo do sinal de detonação é proposto e amostras do sinal real são obtidas e com isso, mostra-se que as técnicas propostas rendem eficientes caracterizadores do fenômeno.

The knock phenomenon that happens in internal combustion engines is a constraint against performance in terms of low pollution rates, higher torque requests (which would imply higher delivered power by the engine), mileage as well as it is an annoying source of noise to the user. In Brazil, such phenomenon may be useful if identified accurately, due to the fact that knock has a higher likelihood to happen with gasoline than ethanol. Thus, the occurrence of knock may be a support in an optimized management of fuel injection and sparking time, as well as it may provide means to identify the fuel being used. In order to achieve such goal, signal processing techniques may be used for characterization of such phenomenon. This work has the focus of identifying (occurrence) and characterize (intensity and duration) of knock signal using adaptive filtering and power estimators, where the individual and combined performance are assessed. To begin with, a knock signal model is proposed as well as samples of the real signal are taken, with that, it is shown that the proposed techniques offer efficient detectors of the phenomenon.

Advisors/Committee Members: Antonio Carlos Seabra, Edson Caoru Kitani, Cássio Guimarães Lopes.

Subjects/Keywords: Combustão anormal; Detonação; Estimador de potência; Filtros adaptativos; Sensor de detonação; Abnormal combustion; Adaptive filters; Knock; Knock sensor; Power estimator

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, R. L. d. (2013). Caracterização do sinal do fenômeno de detonação utilizando filtros adaptativos e estimador de potência. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/3/3140/tde-22102014-122132/

Chicago Manual of Style (16^th Edition):

Silva, Rafael Luiz da. “Caracterização do sinal do fenômeno de detonação utilizando filtros adaptativos e estimador de potência.” 2013. Masters Thesis, University of São Paulo. Accessed April 15, 2021. http://www.teses.usp.br/teses/disponiveis/3/3140/tde-22102014-122132/.

MLA Handbook (7^th Edition):

Silva, Rafael Luiz da. “Caracterização do sinal do fenômeno de detonação utilizando filtros adaptativos e estimador de potência.” 2013. Web. 15 Apr 2021.

Vancouver:

Silva RLd. Caracterização do sinal do fenômeno de detonação utilizando filtros adaptativos e estimador de potência. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Apr 15]. Available from: http://www.teses.usp.br/teses/disponiveis/3/3140/tde-22102014-122132/.

Council of Science Editors:

Silva RLd. Caracterização do sinal do fenômeno de detonação utilizando filtros adaptativos e estimador de potência. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/3/3140/tde-22102014-122132/

21. Eino, Atsushi. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. : Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態.

Degree: 博士（医学）, 2016, Niigata University / 新潟大学

URL: http://hdl.handle.net/10191/41924

►

学位の種類: 博士（医学）. 報告番号: 甲第4127号. 学位記番号: 新大院博（医）甲第694号. 学位授与年月日: 平成28年3月23日

Journal of Cell Science. 2015, 128(23), 4453-4461.

Sqstm1 serves as a signaling hub and receptor for selective… (more)

Subjects/Keywords: Sqstm1; Autophagy; Selective autophagy; Nrf2; Knock-in mice

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APA (6^th Edition):

Eino, A. (2016). Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. : Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態. (Thesis). Niigata University / 新潟大学. Retrieved from http://hdl.handle.net/10191/41924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Eino, Atsushi. “Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. : Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態.” 2016. Thesis, Niigata University / 新潟大学. Accessed April 15, 2021. http://hdl.handle.net/10191/41924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Eino, Atsushi. “Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. : Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態.” 2016. Web. 15 Apr 2021.

Vancouver:

Eino A. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. : Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態. [Internet] [Thesis]. Niigata University / 新潟大学; 2016. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/10191/41924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Eino A. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. : Sqstm1-GFP ノックインマウスが明らかにした、オートファジーとストレス条件でのSqstm1 の動態. [Thesis]. Niigata University / 新潟大学; 2016. Available from: http://hdl.handle.net/10191/41924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

22. Zhang, Lianghui. Mechanisms for Phospholipase Cε-Dependent Regulation of Cardiac Hypertrophy.

Degree: PhD, 2013, University of Rochester

URL: http://hdl.handle.net/1802/26774

► To explore the mechanisms of phospholipase Cε (PLCε) signaling in cardiomyocyte hypertrophy, PLCε protein was depleted by siRNA in neonatal rat ventricular myocytes (NRVMs). Endothelin-1… (more)

▼ To explore the mechanisms of phospholipase Cε (PLCε) signaling in cardiomyocyte hypertrophy, PLCε protein was depleted by siRNA in neonatal rat ventricular myocytes (NRVMs). Endothelin-1 (ET-1), norepinephrine (NE), isoproterenol (ISO), and insulin-like growth factor-1 (IGF-1) were chosen as hypertrophic agonists. Expression of Gαq in NRVMs was also testes as a hypertrophic stimulus. We found that depletion of PLCε with siRNA significantly inhibited hypertrophic growth and gene expression induced by all agonists tested as well as Gαq, demonstrating that PLCε plays a convergent role in regulating cardiomyocyte hypertrophy. Our data show that PLC catalytic activity is required for PLCε-dependent regulation of hypertrophy, although PLCε deletion did not change global agonist-induced total inositol phosphate generation, suggesting PLC activity was required locally for PLCε-dependent regulation of hypertrophy. Further, PLCε binds to muscle-specific A kinase anchoring protein (mAKAPβ) – a nuclear membrane scaffold protein in the heart. The binding sites on the interaction surface of PLCε and mAKAPβ were mapped and the domains were expressed in NRVMs to disrupt endogenous binding of PLCε to mAKAPβ, which resulted in significantly reduced agonist or Gq-induced hypertrophy, proving that the perinuclear localization of PLCε was required for its hypertrophic function. Furthermore, we explored downstream effectors of PLCε in regulation of hypertrophy. We found that depletion of PLCε inhibited protein kinase D (PKD) activity stimulated by ET-1 or NE in NRVMs, and disruption of PLCε binding to mAKAPβ also reduces it, demonstrating that nuclear PKD activation required PLCε. Similarly, our data show that PLCε depletion inhibits ET-1 stimulated nuclear Ca2+ release in NRVMs, suggesting that IP3 generated by PLCε-dependent hydrolysis contributed to nuclear calcium signals. To further understand the functional role of PLCε found in cardiomyocyte hypertrophy, inducible and tissue specific PLCε knockout mice were generated. Conditional deletion of PLCε in mouse cardiac myocytes after development protected the heart from pressure overload-induced hypertrophy, which strongly supports our mechanistic data from NRVMs. Collectively, our studies have unveiled a novel and important mechanism of regulating cardiac hypertrophy, which suggest PLCε may be a promising target for pharmacological manipulation.

Subjects/Keywords: Phospholipase Cε; Hypertrophy; Cardiomyocyte; PkD; Nuclear Calcium; Inducible; Knock-out; Mice

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APA (6^th Edition):

Zhang, L. (2013). Mechanisms for Phospholipase Cε-Dependent Regulation of Cardiac Hypertrophy. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/26774

Chicago Manual of Style (16^th Edition):

Zhang, Lianghui. “Mechanisms for Phospholipase Cε-Dependent Regulation of Cardiac Hypertrophy.” 2013. Doctoral Dissertation, University of Rochester. Accessed April 15, 2021. http://hdl.handle.net/1802/26774.

MLA Handbook (7^th Edition):

Zhang, Lianghui. “Mechanisms for Phospholipase Cε-Dependent Regulation of Cardiac Hypertrophy.” 2013. Web. 15 Apr 2021.

Vancouver:

Zhang L. Mechanisms for Phospholipase Cε-Dependent Regulation of Cardiac Hypertrophy. [Internet] [Doctoral dissertation]. University of Rochester; 2013. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/1802/26774.

Council of Science Editors:

Zhang L. Mechanisms for Phospholipase Cε-Dependent Regulation of Cardiac Hypertrophy. [Doctoral Dissertation]. University of Rochester; 2013. Available from: http://hdl.handle.net/1802/26774

University of Oulu

23. Heikkilä, M. (Minna). Development of the adreno-genital system:female sex determination, ovarian and adrenal gland ontogeny regulated by Wnt-4 in mice.

Degree: 2002, University of Oulu

URL: http://urn.fi/urn:isbn:951426844X

► Abstract Although the genetic sex of an embryo is determined at conception by the presence or absence of the Y chromosome, both females and males… (more)

Subjects/Keywords: gonads; ovary; knock out mice

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APA (6^th Edition):

Heikkilä, M. (. (2002). Development of the adreno-genital system:female sex determination, ovarian and adrenal gland ontogeny regulated by Wnt-4 in mice. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:951426844X

Chicago Manual of Style (16^th Edition):

Heikkilä, M (Minna). “Development of the adreno-genital system:female sex determination, ovarian and adrenal gland ontogeny regulated by Wnt-4 in mice.” 2002. Doctoral Dissertation, University of Oulu. Accessed April 15, 2021. http://urn.fi/urn:isbn:951426844X.

MLA Handbook (7^th Edition):

Heikkilä, M (Minna). “Development of the adreno-genital system:female sex determination, ovarian and adrenal gland ontogeny regulated by Wnt-4 in mice.” 2002. Web. 15 Apr 2021.

Vancouver:

Heikkilä M(. Development of the adreno-genital system:female sex determination, ovarian and adrenal gland ontogeny regulated by Wnt-4 in mice. [Internet] [Doctoral dissertation]. University of Oulu; 2002. [cited 2021 Apr 15]. Available from: http://urn.fi/urn:isbn:951426844X.

Council of Science Editors:

Heikkilä M(. Development of the adreno-genital system:female sex determination, ovarian and adrenal gland ontogeny regulated by Wnt-4 in mice. [Doctoral Dissertation]. University of Oulu; 2002. Available from: http://urn.fi/urn:isbn:951426844X

Loughborough University

24. Akma, Tengku N. Miller cycle combustion strategy for downsized gasoline engines.

Degree: PhD, 2017, Loughborough University

URL: http://hdl.handle.net/2134/32421

► In response to the global concerns towards oil scarcity and climate change, the automotive industry is currently focusing on improving fuel economy and reducing exhaust… (more)

▼ In response to the global concerns towards oil scarcity and climate change, the automotive industry is currently focusing on improving fuel economy and reducing exhaust emissions. Modern downsized gasoline engines that come with a package that includes a boosting system, variable valve train and direct fuel injection system is effective for fuel economy improvement and emission reduction. However, the knocking issue becomes severe at high load operations as a result of the high intake boosting pressure. In regard to the part load conditions, the gas exchange process requires extra work to draw in air into the cylinder due to a lower amount of pressure in the intake manifold caused by the restriction of the throttle plate. The Miller cycle is regarded as a potential strategy of knock control for downsized gasoline engines. Extensive works have sought to examine the performance improvement via the Miller cycle, yet only limited research has been conducted on the manner in which it can influence knock suppression. The focus of this thesis is to investigate early and late intake valve closing timings in terms of how they affect the compression process, the ability to suppress engine knock and meet the power output required at high loads for spark-ignited gasoline engines. Apart from that, this research also demonstrates the Miller cycle potential by utilising fully variable valve timing in controlling the load at the part load condition without using a throttle. The early intake valve closing with different valve lifts was tested in order to investigate the impact during the gas exchange process, particularly the pumping losses and the potential to improve fuel economy. This study includes both experimental and simulation studies. A Lotus single-cylinder research engine referred to as SCORE was mainly used for the experimental component of the study. The simulation work was conducted using a one-dimensional spark ignition engine model built in the Ricardo WAVE software for naturally-aspirated and downsized engines. The engine model values are validated against the experimental values from the Lotus SCORE and Lotus SABRE engines. The combustion model with a reduced kinetics mechanism was validated using a Rover K-series engine. A broad matrix of the engine operations has been investigated combining a variety of engine speeds and engine loads. The Miller cycle effects on knock suppression in a downsized engine environment have been investigated in three parts, namely the Miller cycle at different speed-load, knock suppression with extreme Miller cycle, and knock analysis with combustion kinetics. Through the works, the Miller cycle has demonstrated its capability to suppress engine knocking in a more efficient manner as opposed to the standard engine operation. This is contributed by the fact that early and late intake valve closings could affect the end gas condition at the end of the compression stroke, thus making it possible to suppress the engine knocking. The experimental study for controlling load without using…

Subjects/Keywords: 629.25; Miller cycle; Downsized gasoline engine; Knock suppression; Load control

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APA (6^th Edition):

Akma, T. N. (2017). Miller cycle combustion strategy for downsized gasoline engines. (Doctoral Dissertation). Loughborough University. Retrieved from http://hdl.handle.net/2134/32421

Chicago Manual of Style (16^th Edition):

Akma, Tengku N. “Miller cycle combustion strategy for downsized gasoline engines.” 2017. Doctoral Dissertation, Loughborough University. Accessed April 15, 2021. http://hdl.handle.net/2134/32421.

MLA Handbook (7^th Edition):

Akma, Tengku N. “Miller cycle combustion strategy for downsized gasoline engines.” 2017. Web. 15 Apr 2021.

Vancouver:

Akma TN. Miller cycle combustion strategy for downsized gasoline engines. [Internet] [Doctoral dissertation]. Loughborough University; 2017. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/2134/32421.

Council of Science Editors:

Akma TN. Miller cycle combustion strategy for downsized gasoline engines. [Doctoral Dissertation]. Loughborough University; 2017. Available from: http://hdl.handle.net/2134/32421

Brunel University

25. Coates, Barnaby Paul. Investigation of engine design parameters on the efficiency and performance of the high specific power downsized SI engine.

Degree: PhD, 2012, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/12404 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683628

► This study investigates the impact of employing the Miller cycle on a high specific power downsized gasoline engine by means of Early Intake Valve Closing… (more)

▼ This study investigates the impact of employing the Miller cycle on a high specific power downsized gasoline engine by means of Early Intake Valve Closing (EIVC) and Late Intake Valve Closing (LIVC). This investigation assesses the potential for the Miller cycle to improve fuel economy at part load points, as well as high load points with significantly elevated boost pressures (Deep Miller) of up to 4 bar abs. The impact of geometric Compression Ratio (CR) and Exhaust Back Pressure (EBP) has also been investigated. The knock mitigating qualities of Deep Miller have been assessed, and its ability to increase maximum engine load explored. Low Speed Pre-ignition (LSPI) and autoignition tendencies with reduced coolant flow rates and with aged and new fuels have also been studied. This study comprises both experimental and analytical studies. A Ricardo Hydra single cylinder thermodynamic engine was developed and used for the experimental component of the study. This engine features a high specific power output (120kW/l) cylinder head from the Mahle 1.2l 3 cylinder aggressively downsized engine. The analytical component was carried out using a 1-dimensional GT-Power model based on the Ricardo Hydra experimental engine. A Design of Experiments (DoE) based test plan was adopted for this analytical study. The experimental study found that EIVC was the optimal strategy for improving fuel economy at both part-load and high-load conditions. LIVC yielded a fuel economy penalty at part-load operations and a fuel economy improvement at high-loads. The unexpected part-load LIVC result was attributed to the engine breathing dynamics of the experimental engine. The analytical study found moderate LIVC to be the optimal strategy at lower speeds, unless compensation for the increased degree of scavenging experienced with EIVC was compensated for, in which case EIVC was optimum. At higher speeds EIVC was found to be optimum regardless of whether or not compensation for scavenging was employed. It was generally found that less sensitivity to EBP was exhibited the more extreme the EIVC and LIVC. It was also found that a higher geometric CR could be tolerated with extreme EIVC and LIVC, and a fuel economy benefit could be obtained through the elevation of Geometric CR.

Subjects/Keywords: 621.4; Miller; Deep Miller; Knock; Pre-ignition; Over-expansion

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APA (6^th Edition):

Coates, B. P. (2012). Investigation of engine design parameters on the efficiency and performance of the high specific power downsized SI engine. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/12404 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683628

Chicago Manual of Style (16^th Edition):

Coates, Barnaby Paul. “Investigation of engine design parameters on the efficiency and performance of the high specific power downsized SI engine.” 2012. Doctoral Dissertation, Brunel University. Accessed April 15, 2021. http://bura.brunel.ac.uk/handle/2438/12404 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683628.

MLA Handbook (7^th Edition):

Coates, Barnaby Paul. “Investigation of engine design parameters on the efficiency and performance of the high specific power downsized SI engine.” 2012. Web. 15 Apr 2021.

Vancouver:

Coates BP. Investigation of engine design parameters on the efficiency and performance of the high specific power downsized SI engine. [Internet] [Doctoral dissertation]. Brunel University; 2012. [cited 2021 Apr 15]. Available from: http://bura.brunel.ac.uk/handle/2438/12404 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683628.

Council of Science Editors:

Coates BP. Investigation of engine design parameters on the efficiency and performance of the high specific power downsized SI engine. [Doctoral Dissertation]. Brunel University; 2012. Available from: http://bura.brunel.ac.uk/handle/2438/12404 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.683628

Michigan Technological University

26. Luo, Wei. STUDY OF REAL-TIME STOCHASTIC KNOCK DETECTION AND CONTROL FOR SPARK-IGNITION ENGINES.

Degree: PhD, Department of Mechanical Engineering-Engineering Mechanics, 2014, Michigan Technological University

URL: https://digitalcommons.mtu.edu/etd-restricted/286

► The ability to operate a spark-ignition (SI) engine near the knock limit provides a net reduction of engine fuel consumption. This work presents a… (more)

Subjects/Keywords: Control; Detection; Knock; SI Engine; Stochastic; Mechanical Engineering

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APA (6^th Edition):

Luo, W. (2014). STUDY OF REAL-TIME STOCHASTIC KNOCK DETECTION AND CONTROL FOR SPARK-IGNITION ENGINES. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etd-restricted/286

Chicago Manual of Style (16^th Edition):

Luo, Wei. “STUDY OF REAL-TIME STOCHASTIC KNOCK DETECTION AND CONTROL FOR SPARK-IGNITION ENGINES.” 2014. Doctoral Dissertation, Michigan Technological University. Accessed April 15, 2021. https://digitalcommons.mtu.edu/etd-restricted/286.

MLA Handbook (7^th Edition):

Luo, Wei. “STUDY OF REAL-TIME STOCHASTIC KNOCK DETECTION AND CONTROL FOR SPARK-IGNITION ENGINES.” 2014. Web. 15 Apr 2021.

Vancouver:

Luo W. STUDY OF REAL-TIME STOCHASTIC KNOCK DETECTION AND CONTROL FOR SPARK-IGNITION ENGINES. [Internet] [Doctoral dissertation]. Michigan Technological University; 2014. [cited 2021 Apr 15]. Available from: https://digitalcommons.mtu.edu/etd-restricted/286.

Council of Science Editors:

Luo W. STUDY OF REAL-TIME STOCHASTIC KNOCK DETECTION AND CONTROL FOR SPARK-IGNITION ENGINES. [Doctoral Dissertation]. Michigan Technological University; 2014. Available from: https://digitalcommons.mtu.edu/etd-restricted/286

University of Melbourne

27. Wu, Bolong. Identify new regulators of TNFR1-induced necroptotic pathway.

Degree: 2019, University of Melbourne

URL: http://hdl.handle.net/11343/233842

► Acute Myeloid Leukaemia (AML) is a vastly heterogeneous blood disorder with a poor prognosis for patients older than 65. Our group has been focused on… (more)

▼ Acute Myeloid Leukaemia (AML) is a vastly heterogeneous blood disorder with a poor prognosis for patients older than 65. Our group has been focused on developing new treatments for AML to replace the standard intensive chemotherapy. Previous data showed that the SMAC-mimetic birinapant in combination with the caspase inhibitor IDN could kill different types of AML both in vitro and in vivo through activation of necroptosis cell death pathway. However, over 50% of the patient samples tested in study showed resistance to necroptosis. This project aims to determine the molecular mechanisms that mediate necroptosis resistance in AML and identify new regulators of necroptotic pathway. The results obtained in this study will expand the knowledge of necroptosis signalling in leukaemia and will contribute to the optimal clinical use of birinapant/IDN drug combination. This project contains 2 parts; (1) We will use human AML cell lines that are resistant to necroptosis to determine the molecular changes involved in cell death resistance. (2) We will use CRISPR/Cas9 knock out screen in human AML cell lines that are sensitive to necroptosis, trying to identify new regulators of TNFR1-induced necroptotic pathway. Together these experimental approaches will allow a better understanding of the regulation of TNF-necroptosis signalling in AML. By overexpression wild-type RIPK3 in the KG-1 cell line, we successfully sensitised KG- 1 cells to necroptosis, which indicates that the KG-1 endogenous RIPK3 is dysfunctional. By cDNA sequence of KG-1 endogenous RIPK3, we detected several mutant base pairs, which may lead to the dysfunction, but this result needs further prove by genome sequence, which is undergoing. By CRISPR knock out screen, we found several targets that may lead to the necroptotic resistant, and MAGE3 is the most research-worthy one. Knockout MAGEB3 in the MV4;11 cell line led to the downregulation of RIPK3 and the necroptotic resistance. However, this result could not be repeated on the U937 cell line, and the mechanism of how MAGEB3 regulates RIPK3 is still unclear. Further research will be done on MAGEB3 to have a better understanding of the role of MAGEB3 in the necroptotic pathway. Together, all these results gave a better understanding of the necroptotic pathway and may contribute to the treatment of AML.

Subjects/Keywords: Acute Myeloid Leukemia; Necroptosis; CRISPR knock out screen; MAGEB3

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APA (6^th Edition):

Wu, B. (2019). Identify new regulators of TNFR1-induced necroptotic pathway. (Masters Thesis). University of Melbourne. Retrieved from http://hdl.handle.net/11343/233842

Chicago Manual of Style (16^th Edition):

Wu, Bolong. “Identify new regulators of TNFR1-induced necroptotic pathway.” 2019. Masters Thesis, University of Melbourne. Accessed April 15, 2021. http://hdl.handle.net/11343/233842.

MLA Handbook (7^th Edition):

Wu, Bolong. “Identify new regulators of TNFR1-induced necroptotic pathway.” 2019. Web. 15 Apr 2021.

Vancouver:

Wu B. Identify new regulators of TNFR1-induced necroptotic pathway. [Internet] [Masters thesis]. University of Melbourne; 2019. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/11343/233842.

Council of Science Editors:

Wu B. Identify new regulators of TNFR1-induced necroptotic pathway. [Masters Thesis]. University of Melbourne; 2019. Available from: http://hdl.handle.net/11343/233842

Duke University

28. Singh, Sumeet Pal. Mechanisms Underlying Bone Cell Recovery During Zebrafish Fin Regeneration .

Degree: 2013, Duke University

URL: http://hdl.handle.net/10161/8218

► Zebrafish regenerate amputated caudal fins, restoring the size and shape of the original appendage. Regeneration requires generation of diverse cell types comprising the adult… (more)

Subjects/Keywords: Biology; Bone; Knock-in; Plasticity; Regeneration; TALEN; Zebrafish

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APA (6^th Edition):

Singh, S. P. (2013). Mechanisms Underlying Bone Cell Recovery During Zebrafish Fin Regeneration . (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/8218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Singh, Sumeet Pal. “Mechanisms Underlying Bone Cell Recovery During Zebrafish Fin Regeneration .” 2013. Thesis, Duke University. Accessed April 15, 2021. http://hdl.handle.net/10161/8218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Singh, Sumeet Pal. “Mechanisms Underlying Bone Cell Recovery During Zebrafish Fin Regeneration .” 2013. Web. 15 Apr 2021.

Vancouver:

Singh SP. Mechanisms Underlying Bone Cell Recovery During Zebrafish Fin Regeneration . [Internet] [Thesis]. Duke University; 2013. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/10161/8218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Singh SP. Mechanisms Underlying Bone Cell Recovery During Zebrafish Fin Regeneration . [Thesis]. Duke University; 2013. Available from: http://hdl.handle.net/10161/8218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

California State University – Sacramento

29. McClain, Kevin. LabVIEW internal combustion engine detonation frequency analysis.

Degree: MS, Mechanical Engineering, 2011, California State University – Sacramento

URL: http://hdl.handle.net/10211.9/947

► The project discussed herein is to develop the hardware and software necessary to identify the detonation frequency of a BMW S14 internal combustion engine. This… (more)

Subjects/Keywords: Knock detection; Engine fuel maps; Engine timing maps; Fast Fourier transform

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APA (6^th Edition):

McClain, K. (2011). LabVIEW internal combustion engine detonation frequency analysis. (Masters Thesis). California State University – Sacramento. Retrieved from http://hdl.handle.net/10211.9/947

Chicago Manual of Style (16^th Edition):

McClain, Kevin. “LabVIEW internal combustion engine detonation frequency analysis.” 2011. Masters Thesis, California State University – Sacramento. Accessed April 15, 2021. http://hdl.handle.net/10211.9/947.

MLA Handbook (7^th Edition):

McClain, Kevin. “LabVIEW internal combustion engine detonation frequency analysis.” 2011. Web. 15 Apr 2021.

Vancouver:

McClain K. LabVIEW internal combustion engine detonation frequency analysis. [Internet] [Masters thesis]. California State University – Sacramento; 2011. [cited 2021 Apr 15]. Available from: http://hdl.handle.net/10211.9/947.

Council of Science Editors:

McClain K. LabVIEW internal combustion engine detonation frequency analysis. [Masters Thesis]. California State University – Sacramento; 2011. Available from: http://hdl.handle.net/10211.9/947

University of New South Wales

30. Rusly, Alvin Mulianto. The transiency of in-cylinder flame development in an automotive-size diesel engine.

Degree: Mechanical & Manufacturing Engineering, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52828 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11501/SOURCE01?view=true

► Global growth in the sales of light-duty diesel-powered vehicles is effectively driven by diesel engines superior fuel economy though concerns implicating emission formations and usage… (more)

▼ Global growth in the sales of light-duty diesel-powered vehicles is effectively driven by diesel engines superior fuel economy though concerns implicating emission formations and usage of non-renewable fossil still persist. Such obstacles present a need for better understanding of the diesel combustion, which will help improve engine efficiency and reduce pollutant emissions. To address this issue, experimental study of in-cylinder combustion processes is conducted in a light-duty diesel engine with focus on flame development transience. A new experimental research facility has been designed and constructed to study transient behaviour of diesel flames during combustion. The facility houses a modified single-cylinder diesel engine that allows optical access to the combustion chamber at realistic engine environment and ambient conditions. Two distinctly different diesel combustion regimes are investigated: one with short injection duration and the other with long injection duration. The first of the combustion regimes consists of short injection duration and long ignition delay ultimately resulting in a positive ignition dwell (fuel injection completes prior to ignition). In this regime, the overall combustion is dominated by premixed burn phase whereby further improvement of efficiency is limited by a drastic increase in in-cylinder pressure. If the problem is severe, undesirable pressure ringing follows the initial pressure rise, which is called diesel knock. The first part of this thesis addresses this issue of knocking in a light-duty diesel engine. In the optical engine, high-speed natural hot soot luminosity imaging was performed to visualise flame behaviour during the knocking cycles. It is found that the diesel flame oscillates against the normal swirl direction and the oscillation frequency matches the frequency of in-cylinder pressure ringing, which is the first observation of such correspondence in a diesel engine. Experimentation with pilot injection showed a remedial effect through elimination of pressure ringing and dampening of flame oscillation. Such results are connected with a short ignition delay and less intense premixed combustion as shown through a lower pressure rise rate and negative ignition dwell (i.e. mixing-controlled combustion).The second regime investigated in this thesis presents long injection duration through a single-hole injector resulting in a negative ignition dwell (combustion starts prior to the end of injection). This regime is dominated by mixing-controlled combustion phase corresponding to high-load engine operating conditions. Opposed to the short-injection regime with positive ignition dwell, this long-injection regime is characterised by a lifted flame that is under the strong influence of jet-wall interaction during and after the fuel injection. Therefore, the focus of last half of this thesis is on the jet-wall interaction and its impact on lift-off length (i.e. distance between the nozzle to the first detectable flame base within a specified spatial range with respect… Advisors/Committee Members: Kook, Sanghoon, Mechanical & Manufacturing Engineering, Faculty of Engineering, UNSW, Hawkes, Evatt R., Photovoltaics & Renewable Energy Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: Re-entrainment; Diesel; Knock; Lift-off Length; Optical Engine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rusly, A. M. (2013). The transiency of in-cylinder flame development in an automotive-size diesel engine. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52828 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11501/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Rusly, Alvin Mulianto. “The transiency of in-cylinder flame development in an automotive-size diesel engine.” 2013. Doctoral Dissertation, University of New South Wales. Accessed April 15, 2021. http://handle.unsw.edu.au/1959.4/52828 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11501/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Rusly, Alvin Mulianto. “The transiency of in-cylinder flame development in an automotive-size diesel engine.” 2013. Web. 15 Apr 2021.

Vancouver:

Rusly AM. The transiency of in-cylinder flame development in an automotive-size diesel engine. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Apr 15]. Available from: http://handle.unsw.edu.au/1959.4/52828 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11501/SOURCE01?view=true.

Council of Science Editors:

Rusly AM. The transiency of in-cylinder flame development in an automotive-size diesel engine. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/52828 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11501/SOURCE01?view=true

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