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1.
Buitelaar, C.
Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch.
Degree: 2013, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/281034 
► Background: Kisspeptins are recognized to be regulators within the hypothalamus-pituitary-gonadal axis and stimulators of GnRH and gonadotrophin secretion in mammals. In mice and sheep it…
(more)
▼ Background: Kisspeptins are recognized to be regulators within the hypothalamus-pituitary-gonadal axis and stimulators of
GnRH and gonadotrophin secretion in mammals. In mice and sheep it is shown that mRNA expression of the Kiss1 gene and Kiss1R gene, as well as plasma LH concentrations varies between cycle phases. Little is known about sensitivity to
kisspeptin between different cycle phases in the
dog. Therefore the aim of the study is to find out whether there is a difference in sensitivity to
kisspeptin throughout the different stages of the canine estrous cycle and anestrus.
Methods: Beagle bitches were treated intravenously with cKP10 (0,5 µg/kg) during anestrus, follicular
phase and
luteal phase (PIP and PDP). Plasma LH concentrations are measured.
Results: In each experimental
phase, plasma LH concentrations increased significantly after cKP10 administration. LH response peaks were higher during anestrus and PDP compared to follicular
phase and PIP.
Conclusion: LH responses show that sensitivity to
kisspeptin differs during the estrous cycle and anestrus of the Beagle bitch. LH response is high during anestrus and PDP compared to follicular
phase and PIP. More research should be done to confirm our findings and the meaning of high plasma LH response during PDP should be further investigated.
Advisors/Committee Members: Albers-Wolthers, C.H.J..
Subjects/Keywords: Diergeneeskunde; Kisspeptin, dog, GnRH, luteal phase
…luteal phase in the bitch", Theriogenology, vol. 65, no. 7, pp. 1346-1359.
de Roux, N… …difference in sensitivity to kisspeptin during the various stages
of the estrous cycle and anestrus… …in the dog. Results show that intravenous cKP10 administration causes a
raise in plasma LH… …concentration during anestrus and PDP compared to the follicular phase and PIP in the
Beagle bitch… …phase. This could be due to differences in mRNA expression of Kiss1 and Kiss1R.
BIBLIOGRAPHY…
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APA (6th Edition):
Buitelaar, C. (2013). Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/281034
Chicago Manual of Style (16th Edition):
Buitelaar, C. “Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch.” 2013. Masters Thesis, Universiteit Utrecht. Accessed March 06, 2021.
http://dspace.library.uu.nl:8080/handle/1874/281034.
MLA Handbook (7th Edition):
Buitelaar, C. “Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch.” 2013. Web. 06 Mar 2021.
Vancouver:
Buitelaar C. Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Mar 06].
Available from: http://dspace.library.uu.nl:8080/handle/1874/281034.
Council of Science Editors:
Buitelaar C. Stimulation of LH secretion by 0.5 µg/kg cKP10 in various stages of the estrous cycle and anestrus in the Beagle bitch. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/281034
Universiteit Utrecht
2.
Dam, K. van.
“Comparative sequence analysis of canine progesterone receptor-B-Upstream Segment and the relationship of this segment with the estrous cycle in Canidae".
Degree: 2008, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/35787
► The dog seems to have unique features concerning activation function (AF) 3 domain and putative phosphorylation sites of the progesterone receptor-B-Upstream Segment (BUS) in comparison…
(more)
▼ The
dog seems to have unique features concerning activation function (AF) 3 domain and putative phosphorylation sites of the progesterone receptor-B-Upstream Segment (BUS) in comparison with mice, rats, rabbits, horses, cattle and human. The first aim of this research is to assess if the
dog is still unique when compared to wolves, seals, ferrets, cats and pigs. A comparative sequence analyses was performed. In the wolf, the same differences as the
dog were found in the AF3 domain and putative phosphorylation sites. Therefore, the differences found in AF3 domains and putative phosphorylation sites do not seem to be unique for the
dog, but might be unique for wolf-like canids or Canidae in general.
According to many authors, canids or Canidae in general have a unique reproduction cycle. One of the remarkable features of this cycle is the prolonged
luteal phase, during which progesterone is the dominating hormone. The question rises if there is a connection between the unique sequence of BUS and the apparently unique prolonged
luteal phase of Canidae. Answering this question is the second aim of this study. Therefore, a short review was performed. Besides Canidae, also some members of the Ursidae and Phocidae families showed a prolonged
luteal phase. These species were all monoestrous females with spontaneous ovulation. The sequence analysis of the seal showed no changes in the AF3 domain and the changes in the putative phosphorylation sites differed from the changes in the
dog and wolf. Therefore, no connection between BUS and the estrous cycle could be assessed in Canidae.
Advisors/Committee Members: Gracanin, A., Mol, J.A..
Subjects/Keywords: Diergeneeskunde; progesterone receptor, dog, canine, BUS, estrous cycle, AF3 domain, phosphorylation sites, luteal phase, Canidae
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APA (6th Edition):
Dam, K. v. (2008). “Comparative sequence analysis of canine progesterone receptor-B-Upstream Segment and the relationship of this segment with the estrous cycle in Canidae". (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/35787
Chicago Manual of Style (16th Edition):
Dam, K van. ““Comparative sequence analysis of canine progesterone receptor-B-Upstream Segment and the relationship of this segment with the estrous cycle in Canidae".” 2008. Masters Thesis, Universiteit Utrecht. Accessed March 06, 2021.
http://dspace.library.uu.nl:8080/handle/1874/35787.
MLA Handbook (7th Edition):
Dam, K van. ““Comparative sequence analysis of canine progesterone receptor-B-Upstream Segment and the relationship of this segment with the estrous cycle in Canidae".” 2008. Web. 06 Mar 2021.
Vancouver:
Dam Kv. “Comparative sequence analysis of canine progesterone receptor-B-Upstream Segment and the relationship of this segment with the estrous cycle in Canidae". [Internet] [Masters thesis]. Universiteit Utrecht; 2008. [cited 2021 Mar 06].
Available from: http://dspace.library.uu.nl:8080/handle/1874/35787.
Council of Science Editors:
Dam Kv. “Comparative sequence analysis of canine progesterone receptor-B-Upstream Segment and the relationship of this segment with the estrous cycle in Canidae". [Masters Thesis]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/35787
University of Otago
3.
de Croft, Simon.
Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse
.
Degree: University of Otago
URL: http://hdl.handle.net/10523/5611
► The gonadotropin-releasing hormone (GnRH) neurons are the principal neurons in a network within the hypothalamus that controls fertility in all mammals. GnRH neuron output is…
(more)
▼ The gonadotropin-releasing hormone (
GnRH) neurons are the principal neurons in a network within the hypothalamus that controls fertility in all mammals.
GnRH neuron output is influenced by a wide range of neurotransmitters. One recently-discovered and essential neurotransmitter in this network is known as
kisspeptin.
Kisspeptin neurons exist in two distinct populations; in the rostral periventricular nucleus of the third ventricle (RP3V) and in the arcuate nucleus (ARN). Based upon anatomical evidence, the RP3V
kisspeptin neurons are hypothesized to be important for the positive feedback mechanism of estrogen that drives the preovulatory
GnRH surge. Also, anatomical evidence has led to the suggestion that the ARN
kisspeptin neuronal population is involved in the negative feedback mechanism that homeostatically suppresses
GnRH release and also is important to drive pulsatile
GnRH secretion.
In my PhD, I have performed experiments to gain functional insight into the roles of these two
kisspeptin neuronal populations. To identify
kisspeptin neurons, I have used a knock-in
kisspeptin-GFP mouse line, in which only
kisspeptin neurons should appear fluorescent. I used immunohistochemistry to show that
kisspeptin neurons can be identified in vitro using GFP fluorescence.
I have then used patch-clamp electrophysiology techniques to describe the spontaneous firing rate of
kisspeptin neurons and described this across the estrous cycle. I demonstrate that the spontaneous electrical activity is not strongly
subject to fluctuating gonadal steroid levels. Unexpectantly, I have described a male-dominant sex difference in spontaneous firing rate of ARN
kisspeptin neurons. Additionally, I show that RP3V
kisspeptin neurons are not strongly activated at the time of the
GnRH surge when measured in an acute brain slice.
ARN
kisspeptin neurons are thought to form an interconnected network, and utilize coexpressed neuropeptides to generate a pulsatile output that is important for generating the pulsatile pattern of
GnRH release from the hypothalamus. I have used cell-attached and whole-cell electrophysiological recording techniques to show that neurokinin B and dynorphin potently modulate ARN
kisspeptin neuronal output in an opposing manner, and described the receptors that mediate these effects.
Following this, I have used whole-cell electrophysiological recording techniques to test for functional connections between ARN
kisspeptin neurons. Whereas no evidence was found for monosynaptic glutamatergic synaptic connections, some evidence was found for neuropeptidergic neurotransmission between ARN
kisspeptin neurons.
These results provide electrophysiological data to inform and update theories of
kisspeptin neuronal function which are built largely on anatomical data.
Advisors/Committee Members: Herbison, Allan (advisor).
Subjects/Keywords: Kisspeptin;
Hypothalamus;
GnRH
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APA ·
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APA (6th Edition):
de Croft, S. (n.d.). Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/5611
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
de Croft, Simon. “Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse
.” Doctoral Dissertation, University of Otago. Accessed March 06, 2021.
http://hdl.handle.net/10523/5611.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
de Croft, Simon. “Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse
.” Web. 06 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
de Croft S. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse
. [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10523/5611.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
de Croft S. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse
. [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/5611
Note: this citation may be lacking information needed for this citation format:
No year of publication.
4.
Crossard, Elodie.
Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.
Degree: Docteur es, Sciences de la vie, 2011, Université François-Rabelais de Tours
URL: http://www.theses.fr/2011TOUR4026
► Le kisspeptine (kp) est un peptide, dérivé du gène kiss-1, jouant un rôle majeur dans le contrôle central de la fonction de reproduction en régulant…
(more)
▼ Le kisspeptine (kp) est un peptide, dérivé du gène kiss-1, jouant un rôle majeur dans le contrôle central de la fonction de reproduction en régulant la sécrétion du GnRH chez l’adulte mais également au cours du développement. Les neurones exprimant kiss-1 sont situés dans la région rostrale périventriculaire du 3ème ventricule (RP3V) et le noyau arqué (ARC). L’expression de kiss-1 est hautement régulée par les stéroides sexuels, positivement dans RP3V et négativement dans ARC. Ces deux populations de neurones à kp semblent avoir des rôles différents. Les neurones à kp du RP3V seraient impliqués dans la genèse du pic préovulatoire et ceux de l’ARC dans la régulation de la sécrétion pulsatile de GnRH.L’objectif de la thèse était de déterminer la période de neurogenèse des neurones à kp ainsi que les variations de l’expression de kiss-1 et de kp dans ces deux régions au cours des différentes phases du développement chez le rat mâle et femelle.Nos résultats ont permis de cibler les périodes clés de l’ontogenèse des neurones à kp en montrant 1) que les neurones à kp de l’ARC naissent sur une période étendue à partir du jour embryonnaire (E)12,5; 2) l’existence d’une sous- expression péri-natale du kp dans l’ARC indépendante du sexe; 3) la mise en place, en période néonatale, de différences sexuelles dans les niveaux d’expression et la distribution neuroanatomique du kp; 4) l’existence de régulations péri-pubertaires de kp, dépendantes du sexe et de la région ; 5) la présence de fibres à kp dans des régions hypothalamiques suggère un rôle de kp au-delà de la fonction de reproduction.
Kisspeptin (kp) is a neuropeptide, derived from the kiss-1 gene, which plays a key role in the central control of reproduction by regulating GnRH secretion in adult but also during development. Cells which express kiss-1 are localized in two distincts hypothalamic regions: the rostral peri-ventricular third ventricule area (RP3V) and the arcuate nucleus (ARC). Kiss-1 expression is highly regulated by sex steroids: positively in the RP3V and negatively in the ARC. RP3V kp neurons have been implicated in the pre-ovulatory GnRH surge whereas ARC kp neurons may predominantly act on GnRH secretion pulsatility. The aim of this PhD work was to determine the neurogenesis period of kp neurons and changes of kiss-1 and kp expression in both regions during different stages of development in rats. Our results highlight key periods of kp neurons ontogenesis and show that: 1) ARC kp neurons are born during an extended embryonic neurogenesis period starting at embryonic day (E) 12,5; 2) a sex independent down-regulation of kp occurs during peri-natal period; 3) sex difference in the expression level and neuroanatomique distribution of kp establishes during neo-natal period; 4) kp was regulated during peri-pubertal period in sex and region dependant manner; 5) kp-ir fibers are detected throughout the septo-hypothalamic continuum suggesting that kp could be implicated in other functions than reproductive function.
Advisors/Committee Members: Duittoz, Anne (thesis director), Franceschini-Laurent, Isabelle (thesis director).
Subjects/Keywords: GnRH; Kisspeptin; Rat; Neurogenesis; Sex differentiation
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APA ·
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APA (6th Edition):
Crossard, E. (2011). Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2011TOUR4026
Chicago Manual of Style (16th Edition):
Crossard, Elodie. “Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.” 2011. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 06, 2021.
http://www.theses.fr/2011TOUR4026.
MLA Handbook (7th Edition):
Crossard, Elodie. “Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.” 2011. Web. 06 Mar 2021.
Vancouver:
Crossard E. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2011. [cited 2021 Mar 06].
Available from: http://www.theses.fr/2011TOUR4026.
Council of Science Editors:
Crossard E. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2011. Available from: http://www.theses.fr/2011TOUR4026
5.
Francou, Bruno.
Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms.
Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2016, Université Paris-Saclay (ComUE)
URL: http://www.theses.fr/2016SACLS101
► Les hypogonadismes hypogonadotropes congénitaux (CHH) sont des maladies héréditaires caractérisées par un déficit de sécrétion des gonadotrophines par l’hypophyse, à l’origine d’une infertilité ou d’une…
(more)
▼ Les hypogonadismes hypogonadotropes congénitaux (CHH) sont des maladies héréditaires caractérisées par un déficit de sécrétion des gonadotrophines par l’hypophyse, à l’origine d’une infertilité ou d’une absence complète de puberté. On distingue les formes isolées avec olfaction normale (nCHH) et les formes syndromiques associant au déficit gonadotrope d’autres signes, tel qu’un défaut d’olfaction dans le cas du syndrome de Kallmann (SK), la forme plus fréquente de CHH. Les gènes identifiés dans le SK participent au développement embryonnaire et les gènes des nCHH sont impliqués dans la régulation de la sécrétion de la GnRH ou de son action. A ce stade, deux populations de neurones hypothalamiques gonadotropes sont connues, le neurone à GnRH et le neurone KNDy, sécrétant les Kisspeptines et la Neurokinine B. On estimait que l’ensemble des gènes identifiés couvraient moins de 20% des étiologies génétiques. L’objectif de ce doctorat était d’étudier prévalences et mécanismes physiopathologiques des gènes connus et d’identifier de nouvelles étiologies génétiques de CHH. Dans la première partie, nous avons caractérisé la fonctionnalité de tous les variants identifiés sur les gènes KISS1R, TACR3 et TAC3. Cela a permis de préciser les prévalences chez 600 patients, d’identifier un profil neuroendocrinien propre à l’altération de la signalisation Neurokinine B et de démontrer l’implication des Kisspeptines au cours de la vie embryonnaire. Enfin, nous proposons un modèle d’interaction entre le neurone à GnRH et le neurone KNDy. Dans la seconde partie, nous avons identifié deux nouveaux gènes, SEMA3A dans une forme familiale de SK et PNPLA6 dans une forme familiale rare de CHH syndromique. En conclusion, notre connaissance accrue des formes génétiques de CHH, a permis de développer un panel d’exome ciblé dédié au diagnostic par séquençage nouvelle génération permettant l’analyse simultanée de gènes candidats et de gènes connus.
Congenital hypogonadotropic hypogonadism (CHH) is characterized by deficient or absent pubertal development due to deficient or absent secretion of the pituitary gonadotropins. The many known genetic causes are generally classified into distinct nosological groups. One comprises abnormalities that affect the pre-natal development or migration of GnRH neurons, the paradigm of which is Kallmann syndrome. The other encompasses molecular abnormalities that only affect hypothalamic GnRH synthesis, GnRH release or GnRH signaling at pituitary level. At this stage, two populations of hypothalamic neurons implicated in a gonadotrop function are identified, GnRH neurons and KNDy neurons secreting kisspeptins and neurokinin B. All of the identified genes would represent less than 20% of genetic etiologies.The aim of this PhD was to study the prevalence and pathophysiology mechanisms of known genes and to identify new genetic etiologies of CHH.In the first part, we characterized the function of all molecular events identified on KISS1R, TACR3 and TAC3 genes. Prevalences were estimated in 600 patients. A…
Advisors/Committee Members: Guiochon-Mantel, Anne (thesis director).
Subjects/Keywords: Hypogonadisme; Génétique; Caractérisation fonctionnelle; GnRH; Kallmann; Kisspeptine; Hypogonadism; Genetics; Functionnal characterization; GnRH; Kallmann; Kisspeptin
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APA ·
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APA (6th Edition):
Francou, B. (2016). Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLS101
Chicago Manual of Style (16th Edition):
Francou, Bruno. “Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 06, 2021.
http://www.theses.fr/2016SACLS101.
MLA Handbook (7th Edition):
Francou, Bruno. “Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms.” 2016. Web. 06 Mar 2021.
Vancouver:
Francou B. Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Mar 06].
Available from: http://www.theses.fr/2016SACLS101.
Council of Science Editors:
Francou B. Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLS101
University of Otago
6.
Yeo, Shel-Hwa.
Role of arcuate nucleus kisspeptin neurons in mediating oestrogen negative feedback on gonadotrophin-releasing hormone neurons in adult mouse
.
Degree: 2012, University of Otago
URL: http://hdl.handle.net/10523/2586
► Reproduction and fertility in mammals is critically dependent on the neurosecretion of gonadotrophin-releasing hormone (GnRH) released by the hypothalamic GnRH neurons into the hypophyseal portal…
(more)
▼ Reproduction and fertility in mammals is critically dependent on the neurosecretion of gonadotrophin-releasing hormone (
GnRH) released by the hypothalamic
GnRH neurons into the hypophyseal portal system of the anterior pituitary.
GnRH binds to
GnRH receptors on the pituitary gonadotrophs and directs gonadotrophin secretion into the peripheral circulation. The oestrogen negative feedback mechanism is important in controlling the fluctuating profiles of pulsatile
GnRH and luteinizing hormone (LH) release. For the major part of the oestrous cycle, oestrogen exerts negative feedback actions on both
GnRH neurons and pituitary gonadotrophs to suppress
GnRH release and ultimately restrain LH secretion at its nadir. The precise neurobiological mechanisms underlying oestrogen’s inhibitory effects on
GnRH/LH release are largely unknown. It is hypothesised that
kisspeptin neurons are required to mediate oestrogen negative feedback actions to suppress pulsatile
GnRH/LH secretion. This thesis focused on investigating the role of arcuate nucleus (ARN)
kisspeptin neurons in mediating oestrogen negative feedback actions on
GnRH neurons in adult female mice.
Anterograde tract tracing was conducted to determine the projections of both ARN and rostral periventricular area of the third ventricle (RP3V)
kisspeptin neurons. Retrograde tracing was performed to verify the axonal projections of ARN and RP3V
kisspeptin neurons into the rostral preoptic area and ARN, respectively. The ARN
kisspeptin neurons were found to project widely within both the medial and lateral aspects of the hypothalamus and associated limbic structures, and possibly innervate
GnRH neuron cell bodies or dendrites in the rostral preoptic area. The RP3V
kisspeptin neurons exhibited a more medial projection pattern throughout the brain and were found to provide substantial inputs into the ARN. The diversity of brain regions targeted by
kisspeptin neurons suggests their involvement in regulating multiple circuits in addition to the
GnRH neuronal network. Despite the wide projections of
kisspeptin neurons into various regions, none of the
kisspeptin neuron populations project to the external zone of the median eminence (ME). Together, these data indicate that ARN and RP3V
kisspeptin neurons do not innervate
GnRH nerve terminals in the external zone of the ME. It is possible that ARN
kisspeptin neurons regulate
GnRH nerve terminals through a nonsynaptic interaction, whereby the
kisspeptin may be released from terminals in the internal zone of the ME and act through volume transmission to eventually reach and control
GnRH release in the external zone.
Transgenic mouse models harbouring global and neuron-specific deletions of Gpr54 gene were examined for oestrogen negative feedback dynamics to assess the role of
kisspeptin-Gpr54 signalling in mediating oestrogen negative feedback. Both animal models exhibited abnormal oestrogen negative feedback profiles with the absence of postovariectomy LH elevations. This implied that
kisspeptin neuron input may be…
Advisors/Committee Members: Herbison, Allan (advisor).
Subjects/Keywords: arcuate nucleus;
kisspeptin;
oestrogen;
negative feedback;
GnRH;
LH
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APA (6th Edition):
Yeo, S. (2012). Role of arcuate nucleus kisspeptin neurons in mediating oestrogen negative feedback on gonadotrophin-releasing hormone neurons in adult mouse
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/2586
Chicago Manual of Style (16th Edition):
Yeo, Shel-Hwa. “Role of arcuate nucleus kisspeptin neurons in mediating oestrogen negative feedback on gonadotrophin-releasing hormone neurons in adult mouse
.” 2012. Doctoral Dissertation, University of Otago. Accessed March 06, 2021.
http://hdl.handle.net/10523/2586.
MLA Handbook (7th Edition):
Yeo, Shel-Hwa. “Role of arcuate nucleus kisspeptin neurons in mediating oestrogen negative feedback on gonadotrophin-releasing hormone neurons in adult mouse
.” 2012. Web. 06 Mar 2021.
Vancouver:
Yeo S. Role of arcuate nucleus kisspeptin neurons in mediating oestrogen negative feedback on gonadotrophin-releasing hormone neurons in adult mouse
. [Internet] [Doctoral dissertation]. University of Otago; 2012. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10523/2586.
Council of Science Editors:
Yeo S. Role of arcuate nucleus kisspeptin neurons in mediating oestrogen negative feedback on gonadotrophin-releasing hormone neurons in adult mouse
. [Doctoral Dissertation]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2586
Universiteit Utrecht
7.
Smeets, J.A.S.
Timing of puberty - Which factors trigger pulsatile GnRH release and the onset of puberty?.
Degree: 2015, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/311317
► Puberty is the process of physical and psychological development towards adulthood, ultimately marked by the ability to reproduce. This development requires activation at all levels…
(more)
▼ Puberty is the process of physical and psychological development towards adulthood, ultimately marked by the ability to reproduce. This development requires activation at all levels of the hypothalamus-pituitary-gonadal axis (HPG-axis): the hypothalamus for pulsatile secretion of gonadotropin-releasing hormone (
GnRH), the pituitary for the pulsatile release of gonadotrophic factors and the gonads for generating gametes and gonadal steroids in response to these pulses. Pulsatile
GnRH release from the hypothalamus is the primary drive to the HPG-axis. It seems that this is the limiting factor for the initiation of puberty. This system has the full potential to function at birth, but is being held in check. Interestingly, several direct and indirect upstream signaling pathways regulate the
GnRH-secreting neurons: such as
kisspeptin, leptin and gonadal steroids. Changes in these upstream factors might explain the initiation of pulsatile
GnRH secretion and pubertal development. However, despite extensive research, it remains a mystery as to what exactly triggers the sudden onset of puberty. Furthermore, a clear sex difference exists in the presentation of puberty, occurring 1-2 years earlier in girls. This suggests that underlying mechanisms controlling
GnRH secretion are differentially regulated in both sexes. The current review aims to give a critical overview of recent findings on factors that contribute to the initiation of puberty. Mechanisms that could underlie the sex difference in onset of puberty will be highlighted. New lines of research and remaining questions in the field will be discussed.
Advisors/Committee Members: Bakker, J., Oosting, R..
Subjects/Keywords: Puberty onset; pubertal development; sex difference; GnRH pulses; kisspeptin; leptin
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APA (6th Edition):
Smeets, J. A. S. (2015). Timing of puberty - Which factors trigger pulsatile GnRH release and the onset of puberty?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/311317
Chicago Manual of Style (16th Edition):
Smeets, J A S. “Timing of puberty - Which factors trigger pulsatile GnRH release and the onset of puberty?.” 2015. Masters Thesis, Universiteit Utrecht. Accessed March 06, 2021.
http://dspace.library.uu.nl:8080/handle/1874/311317.
MLA Handbook (7th Edition):
Smeets, J A S. “Timing of puberty - Which factors trigger pulsatile GnRH release and the onset of puberty?.” 2015. Web. 06 Mar 2021.
Vancouver:
Smeets JAS. Timing of puberty - Which factors trigger pulsatile GnRH release and the onset of puberty?. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Mar 06].
Available from: http://dspace.library.uu.nl:8080/handle/1874/311317.
Council of Science Editors:
Smeets JAS. Timing of puberty - Which factors trigger pulsatile GnRH release and the onset of puberty?. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/311317
University of Michigan
8.
Adams, Caroline.
Integrating Network and Intrinsic Changes in GnRH Neuron Control of Ovulation.
Degree: PhD, Mol & Integrtv Physiology PhD, 2020, University of Michigan
URL: http://hdl.handle.net/2027.42/155290
► Infertility affects 15-20% of couples; failure to ovulate is a common cause. Ovulation is triggered when estradiol switches from negative feedback action on the pituitary…
(more)
▼ Infertility affects 15-20% of couples; failure to ovulate is a common cause. Ovulation is triggered when estradiol switches from negative feedback action on the pituitary and hypothalamus to positive feedback, initiating a surge of gonadotropin-releasing hormone (
GnRH) secretion that causes a surge of luteinizing hormone (LH) release, which triggers ovulation. Our understanding of the neurobiological changes underlying the switch from negative to positive feedback is incomplete. High levels of estradiol are essential, and in rodents, the LH surge tends to occur at a specific time-of-day.
GnRH neurons, however, do not express the estrogen receptor required for feedback, thus estradiol-sensitive afferents likely convey estradiol information to
GnRH neurons. We hypothesized that
GnRH neurons switch from negative to positive feedback by integrating multiple changes to their synaptic inputs and intrinsic properties.
To investigate the neurobiological mechanisms that underlie surge generation, daily
GnRH/LH surges can be induced by ovariectomy and estradiol replacement (OVX+E) in rodents.
GnRH neuron activity and release are increased in the afternoon (positive feedback) and decreased in the morning (negative feedback). No time-of-day changes are observed in OVX mice that do not receive an estradiol implant. Previous studies using the daily surge model have elucidated multiple
GnRH neuron intrinsic and fast-synaptic changes during the switch from negative to positive feedback. It is unclear which if any of these changes are necessary for increasing
GnRH firing rate during positive feedback. We hypothesized that changes to
GnRH neuron intrinsic properties culminate in an increase in excitability to current steps during positive feedback and a decrease in excitability during negative feedback. To our surprise, changes to
GnRH neuron ionic conductances rendered
GnRH neurons more excitable during positive feedback relative to all other groups, but changes to ionic conductances between OVX and negative feedback animals had no net effect on
GnRH neuron excitability. A mathematical model using a novel application of a rigorous parameter estimation method predicted that multiple, redundant combinations of changes to
GnRH intrinsic conductances can produce the firing response in positive feedback. Changes to two interdependent parameters that determine the kinetics of voltage-gated potassium channels accounted for the similar neural responses during negative feedback and in OVX mice.
Although enhancing
GnRH neuron excitability is expected to increase firing rate during positive feedback, it is unclear if this change is necessary or if the concomitant increase is fast-synaptic transmission is sufficient for increasing
GnRH neural activity during positive feedback. To test this, we used dynamic clamp to inject positive feedback, negative feedback, and OVX postsynaptic conductance trains into cells from positive feedback, negative feedback, and OVX mice. Positive feedback conductance trains were more effective in initiating spiking in…
Advisors/Committee Members: Moenter, Sue (committee member), Schnell, Santiago David (committee member), Booth, Victoria (committee member), Elias, Carol (committee member), Forger, Daniel Barclay (committee member), Murphy, Geoffrey G (committee member).
Subjects/Keywords: GnRH; kisspeptin; estradiol feedback; Markov-chain Monte Carlo method; Physiology; Science
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APA (6th Edition):
Adams, C. (2020). Integrating Network and Intrinsic Changes in GnRH Neuron Control of Ovulation. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/155290
Chicago Manual of Style (16th Edition):
Adams, Caroline. “Integrating Network and Intrinsic Changes in GnRH Neuron Control of Ovulation.” 2020. Doctoral Dissertation, University of Michigan. Accessed March 06, 2021.
http://hdl.handle.net/2027.42/155290.
MLA Handbook (7th Edition):
Adams, Caroline. “Integrating Network and Intrinsic Changes in GnRH Neuron Control of Ovulation.” 2020. Web. 06 Mar 2021.
Vancouver:
Adams C. Integrating Network and Intrinsic Changes in GnRH Neuron Control of Ovulation. [Internet] [Doctoral dissertation]. University of Michigan; 2020. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/2027.42/155290.
Council of Science Editors:
Adams C. Integrating Network and Intrinsic Changes in GnRH Neuron Control of Ovulation. [Doctoral Dissertation]. University of Michigan; 2020. Available from: http://hdl.handle.net/2027.42/155290
University of Arizona
9.
Rometo, Adonna Marie.
Ovarian Steroid Modulation of Neuropeptide Gene Expression and Neuronal Morphology in the Primate Hypothalamus
.
Degree: 2008, University of Arizona
URL: http://hdl.handle.net/10150/194500
► In the United States, there are currently more than 40 million postmenopausal women. These women are faced with a variety of physiological changes including ovarian…
(more)
▼ In the United States, there are currently more than 40 million postmenopausal women. These women are faced with a variety of physiological changes including ovarian steroid withdrawal and alterations in hypothalamic neurons. Within the hypothalamic infundibular nucleus of postmenopausal women, there is neuronal hypertrophy and an increase in neurokinin B gene expression. Recent studies identified the kisspeptins and dynorphins as major regulators of reproduction. In our first experiment, we examined the location and alterations of KiSS-1 mRNA-expressing neurons in the hypothalami of pre and postmenopausal women. KiSS-1 neurons were largely confined to the infundibular nucleus, and in postmenopausal women, exhibited neuronal hypertrophy and increased gene expression. To determine if these changes could result from alterations in ovarian steroids, we investigated KiSS-1 gene expression in the hypothalamic infundibular nucleus of non-human primates. Similar to the findings in postmenopausal women, ovariectomy of monkeys resulted in neuronal hypertrophy and increased KiSS-1 gene expression within the infundibular nucleus. Further, estrogen treatment of ovariectomized monkeys yielded a dramatic decrease in KiSS-1 gene expression. Together, these findings suggest that the postmenopausal alterations in KiSS-1 neurons are secondary to ovarian failure.In a second study, we examined alterations in dynorphin gene expression in the hypothalami of pre and postmenopausal women. Dynorphin mRNA-expressing neurons were identified in multiple nuclei. Numbers of dynorphin neurons were decreased within the mPOA and infundibular nucleus of postmenopausal women. In the infundibular nucleus of postmenopausal women, dynorphin neurons were hypertrophied. To determine the contribution of ovarian steroids on dynorphin gene expression, we examined dynorphin mRNA in a monkey model of menopause. Young ovariectomized monkeys exhibited hypertrophy of dynorphin neurons, with no changes in dynorphin gene expression. Estrogen replacement yielded a decrease in neuronal size and an increase in dynorphin neuron number.In future studies, we will use Quantum Dot FISH to determine if NKB, KiSS-1, and dynorphin are colocalized in the hypertrophied neurons. These neuropeptides are involved in the regulation of
GnRH and changes in their gene expression likely contribute to postmenopausal alterations in reproductive hormones. Our findings provide greater understanding of the postmenopausal condition and offer opportunities for pharmaceutical investigation and treatment.
Advisors/Committee Members: Rance, Naomi E (advisor), Hoyer, Patricia (committeemember), Levine, Rick (committeemember), Vanderah, Todd (committeemember).
Subjects/Keywords: Menopause;
Kisspeptin;
Dynorphin;
GnRH;
Primate
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APA (6th Edition):
Rometo, A. M. (2008). Ovarian Steroid Modulation of Neuropeptide Gene Expression and Neuronal Morphology in the Primate Hypothalamus
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/194500
Chicago Manual of Style (16th Edition):
Rometo, Adonna Marie. “Ovarian Steroid Modulation of Neuropeptide Gene Expression and Neuronal Morphology in the Primate Hypothalamus
.” 2008. Doctoral Dissertation, University of Arizona. Accessed March 06, 2021.
http://hdl.handle.net/10150/194500.
MLA Handbook (7th Edition):
Rometo, Adonna Marie. “Ovarian Steroid Modulation of Neuropeptide Gene Expression and Neuronal Morphology in the Primate Hypothalamus
.” 2008. Web. 06 Mar 2021.
Vancouver:
Rometo AM. Ovarian Steroid Modulation of Neuropeptide Gene Expression and Neuronal Morphology in the Primate Hypothalamus
. [Internet] [Doctoral dissertation]. University of Arizona; 2008. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10150/194500.
Council of Science Editors:
Rometo AM. Ovarian Steroid Modulation of Neuropeptide Gene Expression and Neuronal Morphology in the Primate Hypothalamus
. [Doctoral Dissertation]. University of Arizona; 2008. Available from: http://hdl.handle.net/10150/194500
University of Edinburgh
10.
George, Jyothis Thomas.
Functional characterisation and translational applications of kisspeptin-10.
Degree: PhD, 2012, University of Edinburgh
URL: http://hdl.handle.net/1842/9432
► Background: Kisspeptins, recently discovered hypothalamic neuropeptides encoded by the KISS1 gene, are essential for normal pubertal development and are modulated by diverse endocrine, metabolic and…
(more)
▼ Background: Kisspeptins, recently discovered hypothalamic neuropeptides encoded by the KISS1 gene, are essential for normal pubertal development and are modulated by diverse endocrine, metabolic and environmental signals. Exogenous kisspeptin administration potently stimulates LH secretion - by direct action on GnRH neurons while kisspeptin antagonists inhibit pulsatile LH secretion. Human studies of kisspeptin had hitherto used kisspeptin-54 that is cleaved further and the smallest bioactive form is a decapeptide (kisspeptin-10) with a shorter half-life. Kisspeptin-10 is thus putatively more attractive in studies assessing LH pulsatility and is also the basis for the development of antagonists. Unmet clinical needs: Decreased LH pulse frequency is the central pathology in pubertal delay, late-onset male hypogonadism and hypothalamic amenorrhoea. Manipulation of LH pulse frequency also has therapeutic potential in contraception, PCOS and sex-steroid dependant diseases such as endometriosis and prostatic hyperplasia. Hypothesis: That exogenous kisspeptin-10 enhances pulsatile LH secretion in healthy men and in patients with reproductive disorders associated with decreased pulse frequency. Research strategy: A first-in-human dose escalation study of kisspeptin-10 was performed in men and subsequently replicated in women. An intravenous infusion regime was optimised in healthy men and subsequently applied to hypogonadal patients. Specific questions were addressed sequentially as summarised below with key results. Dose escalation study: Question: Does kisspeptin-10 stimulate LH secretion in men? Findings: Six iv bolus doses (0.01 to 3 μg/kg) of GMP kisspeptin-10 and vehicle were administered at least a week apart to six healthy men. Rapid increase in LH, with peak concentrations was seen by 45 min post injection in all volunteers. There was a clear dose-dependent increase in LH concentrations in response to kisspeptin- 10 (P <0.0001). Area-Under-Curve analysis over 60 min following kisspeptin-10 administration showed 0.3 and 1μg/kg doses to be maximally stimulatory (P <0.01) with a reduced response at 3 μg/kg. Assessing the effect of steroid milieu: Question: Steroid feedback is central to the regulation of LH secretion: what effect does the steroid milieu have on LH responses to kisspeptin-10? Findings: The response to iv kisspeptin-10 (0.3μg/kg,) in the normal follicular phase (n=10) was compared with that in the presence of low endogenous sex steroids/high LH secretion (6 postmenopausal women) and in women taking combined contraceptive therapy (n=8) with suppressed LH secretion. Despite widely varying baseline secretion, LH increased significantly following kisspeptin-10 administration in the follicular phase (6.3±1.2 to 9.4±1.3 IU/L P=0.006), postmenopausal (35.3±2.8 to 44.7±3.4 IU/L P=0.005), etonogestrel (4.6±0.2 to 7.5±0.9 IU/L, P=0.02), and COCP groups (2.2±0.9 to 3.7±1.4 IU/L P<0.001). Pulse frequency study: Question: GnRH and LH secretion are pulsatile: can kisspeptin-10 enhance LH pulsatility? Findings: Four…
Subjects/Keywords: 616.4; kisspeptin; GnRH; hormone; Luteinizing; follicle stimulating hormones; testosterone
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APA ·
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APA (6th Edition):
George, J. T. (2012). Functional characterisation and translational applications of kisspeptin-10. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/9432
Chicago Manual of Style (16th Edition):
George, Jyothis Thomas. “Functional characterisation and translational applications of kisspeptin-10.” 2012. Doctoral Dissertation, University of Edinburgh. Accessed March 06, 2021.
http://hdl.handle.net/1842/9432.
MLA Handbook (7th Edition):
George, Jyothis Thomas. “Functional characterisation and translational applications of kisspeptin-10.” 2012. Web. 06 Mar 2021.
Vancouver:
George JT. Functional characterisation and translational applications of kisspeptin-10. [Internet] [Doctoral dissertation]. University of Edinburgh; 2012. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1842/9432.
Council of Science Editors:
George JT. Functional characterisation and translational applications of kisspeptin-10. [Doctoral Dissertation]. University of Edinburgh; 2012. Available from: http://hdl.handle.net/1842/9432
11.
Shitano, Fuki.
MR appearance of normal uterine endometrium considering menstrual cycle: differentiation with benign and malignant endometrial lesions : 月経周期を考慮した正常子宮内膜のMR所見：子宮内膜病変との鑑別.
Degree: 博士(医学), 2016, Kyoto University / 京都大学
URL: http://hdl.handle.net/2433/215408
;
http://dx.doi.org/10.14989/doctor.k19582
The final, definitive version of this paper has been published in Acta radiologica by SAGE Publications Ltd, All rights reserved.Final publication is available at http://acr.sagepub.com/
新制・課程博士
甲第19582号
医博第4089号
Subjects/Keywords: uterine endometrium; luteal phase; MRI; endometrial lesions
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APA (6th Edition):
Shitano, F. (2016). MR appearance of normal uterine endometrium considering menstrual cycle: differentiation with benign and malignant endometrial lesions : 月経周期を考慮した正常子宮内膜のMR所見：子宮内膜病変との鑑別. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/215408 ; http://dx.doi.org/10.14989/doctor.k19582
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shitano, Fuki. “MR appearance of normal uterine endometrium considering menstrual cycle: differentiation with benign and malignant endometrial lesions : 月経周期を考慮した正常子宮内膜のMR所見：子宮内膜病変との鑑別.” 2016. Thesis, Kyoto University / 京都大学. Accessed March 06, 2021.
http://hdl.handle.net/2433/215408 ; http://dx.doi.org/10.14989/doctor.k19582.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shitano, Fuki. “MR appearance of normal uterine endometrium considering menstrual cycle: differentiation with benign and malignant endometrial lesions : 月経周期を考慮した正常子宮内膜のMR所見：子宮内膜病変との鑑別.” 2016. Web. 06 Mar 2021.
Vancouver:
Shitano F. MR appearance of normal uterine endometrium considering menstrual cycle: differentiation with benign and malignant endometrial lesions : 月経周期を考慮した正常子宮内膜のMR所見：子宮内膜病変との鑑別. [Internet] [Thesis]. Kyoto University / 京都大学; 2016. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/2433/215408 ; http://dx.doi.org/10.14989/doctor.k19582.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shitano F. MR appearance of normal uterine endometrium considering menstrual cycle: differentiation with benign and malignant endometrial lesions : 月経周期を考慮した正常子宮内膜のMR所見：子宮内膜病変との鑑別. [Thesis]. Kyoto University / 京都大学; 2016. Available from: http://hdl.handle.net/2433/215408 ; http://dx.doi.org/10.14989/doctor.k19582
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Swedish University of Agricultural Sciences
12.
Tasende, Celia.
Pituitary and uterine sex steriod receptors in ewes.
Degree: 2005, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/959/
► The general aim of this research was to gain knowledge of oestrogen and progesterone receptor (ER and PR) expression in the uterus and pituitary gland…
(more)
▼ The general aim of this research was to gain knowledge of oestrogen and progesterone receptor (ER and PR) expression in the uterus and pituitary gland of the ewe in different reproductive stages (postpartum period, seasonal anoestrus and oestrous cycle), as well as in experimentally induced subnormal vs. normal luteal phases in anoestrous ewes. Single, saturable and high-affinity binding sites for both oestrogen (E) and progesterone (P) were demonstrated in all of the tissue samples of the pituitary and the uterus. The values of the apparent dissociation constants (Kd) of ER and PR did not differ between the different postpartum days examined. Likewise the Kd values of ER and PR did not differ between anoestrous ewes, anoestrous treated ewes and cyclic ewes. The similar Kd values found during the different reproductive stages suggest that variations in the sensitivity of these target tissues to the ovarian hormones may not depend on changes in receptor affinity but rather on the binding capacity (number of receptors). During the postpartum period of ewes lambing in the breeding season, both ER and PR concentrations in the uterus were significantly lower in early than in late postpartum. The correlation between PR and ER concentration was positive, while the correlation between uterine weight and the concentration of either steroid receptor was negative. During the late postpartum period the number of ewes with follicles larger than 4 mm (presumptive oestrogen-active follicles) increased. Therefore, the restoration of uterine ER and PR concentrations was temporally associated with the presence of E-active follicles in the ovary. Overall results suggest that E up-regulated the uterine steroid receptor concentrations and these molecular events may be involved in the uterine remodelling in the late postpartum period during the breeding season. In seasonal anoestrous ewes, low pituitary ER and PR concentrations were found; in contrast with the high receptor concentrations found in the uteri of the same animals. However, the ERα mRNA concentrations in both the pituitary gland and the uterus were similar. While P treatment did not affect the pituitary receptor concentrations, it did decrease the uterine receptor concentrations, but it did not affect ERα mRNA concentrations in either the pituitary or the uterus. Treatment with gonadotrophin-releasing hormone (GnRH), with or without P in the anoestrous ewes, increased the pituitary ER and PR concentrations ten fold without affected the uterine receptor concentrations. GnRH treatment (with or without P) increased ERα mRNA concentrations in both the pituitary gland and the uterus. The decreases of uterine steroid receptor concentrations with P treatment, without affecting the ERα mRNA concentrations, suggest that P down-regulation occurs at posttranscriptional level. The results show that regulation of ER and PR concentration by P and GnRH is tissue specific in anoestrous ewes. During the normal oestrous cycle in the breeding season, both pituitary and uterine ER and PR…
Subjects/Keywords: ewes; oestrous cycle; perinatal period; oestrogens; progesterone; gnrh; gonadotropins; hormones; reproduction; sex steroid receptors; postpartum; anoestrous ewes; subnormal luteal phase
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APA (6th Edition):
Tasende, C. (2005). Pituitary and uterine sex steriod receptors in ewes. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/959/
Chicago Manual of Style (16th Edition):
Tasende, Celia. “Pituitary and uterine sex steriod receptors in ewes.” 2005. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed March 06, 2021.
http://pub.epsilon.slu.se/959/.
MLA Handbook (7th Edition):
Tasende, Celia. “Pituitary and uterine sex steriod receptors in ewes.” 2005. Web. 06 Mar 2021.
Vancouver:
Tasende C. Pituitary and uterine sex steriod receptors in ewes. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2005. [cited 2021 Mar 06].
Available from: http://pub.epsilon.slu.se/959/.
Council of Science Editors:
Tasende C. Pituitary and uterine sex steriod receptors in ewes. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2005. Available from: http://pub.epsilon.slu.se/959/
University of Illinois – Urbana-Champaign
13.
Gal, Arnon.
The role of progesterone receptor in hypothalamic kisspeptin neurons.
Degree: PhD, 0361, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/49826
► In recent years, the neuropeptide kisspeptin, secreted by kisspeptin neurons, has been identified as the prime regulator of gonadotropin releasing hormone (GnRH). Kisspeptin neurons express…
(more)
▼ In recent years, the neuropeptide
kisspeptin, secreted by
kisspeptin neurons, has been identified as the prime regulator of gonadotropin releasing hormone (
GnRH).
Kisspeptin neurons express steroid hormone receptors through which gonadal steroids exert both positive and negative feedback to the hypothalamus; estrogen and its receptor, estrogen receptor α (ERα), have been shown to regulate
kisspeptin-mediated
GnRH release through regulation of
kisspeptin expression. However, the roles that the progesterone receptor (Pgr) and the androgen receptor have in progesteronic and androgenic regulation of
kisspeptin-mediated
GnRH release have not been investigated. Previous studies indicated that Pgr has a critical role in the induction of the
GnRH-mediated preovulatory luteinizing hormone (LH) surge. Therefore,
kisspeptin neurons may be the cells in which Pgr mediates this action. Hypersecretion of
GnRH is associated with the development of polycystic ovaries and hyperandrogenemia. The polycystic ovaries are the source of androgens and high level of androgens induces the development of polycystic ovaries. In addition, hyperandrogenemia may increase
GnRH release, potentially through the action on androgen receptor in
kisspeptin neurons. Increased LH level secondary to hypersecretion of
GnRH increases androgen production in the ovary and therefore may lead to a vicious cycle. There is no literature regarding whether the development of polycystic ovaries is structurally reversible, a question that may have great impact on the understanding of the pathogenesis of this condition.
GnRH activates the reproductive axis through induction of gonadotropins release from the pituitary gland; however, quantification of
GnRH is challenging because
GnRH is secreted into the pituitary portal circulation rather than into the general venous circulation. Serum LH level is a good indicator of
GnRH secretion and measurements of serum LH are commonly used for that purpose. Similarly, vaginal exfoliative cytology has been shown to be a good indicator of the cyclic changes in estrogen level throughout the rat estrous cycle. Vaginal exfoliative cytology is commonly used in biomedical and toxicologic studies in mice for the same purpose. Yet, where the rat estrous cycle remains consistently regular from one cycle to the other, the mouse estrous cycle is often irregular. Hence, vaginal exfoliative cytology may not be as good a predictor of the mouse estrous cycle stage as it is in the rat.
The hypotheses in this doctoral research are: 1) progesterone and progesterone receptor (Pgr) regulation on
kisspeptin expression, or androgenic regulation of Pgr and/or
kisspeptin expression increase
kisspeptin-mediated
GnRH secretion. 2) follicular cysts developed by hyperandrogenism disappear when normal hormonal milieu restored. Thus through their steroid hormone receptors, progesterone and androgens may participate in the pathogenesis of female infertility. To test these hypotheses, the following specific aims were developed: 1) compare the vaginal exfoliative…
Advisors/Committee Members: Ko, CheMyong (advisor), Ko, CheMyong (Committee Chair), Bahr, Janice M. (committee member), Wallig, Matthew A. (committee member), Federico, Federico A. (committee member), MacNeill, Amy L. (committee member).
Subjects/Keywords: Progesterone receptor; Kisspeptin neuron; Gonadotropin releasing hormone (GnRH); Regulation; Hypothalamic-Pituitary-Ovarian axis
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APA (6th Edition):
Gal, A. (2014). The role of progesterone receptor in hypothalamic kisspeptin neurons. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/49826
Chicago Manual of Style (16th Edition):
Gal, Arnon. “The role of progesterone receptor in hypothalamic kisspeptin neurons.” 2014. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed March 06, 2021.
http://hdl.handle.net/2142/49826.
MLA Handbook (7th Edition):
Gal, Arnon. “The role of progesterone receptor in hypothalamic kisspeptin neurons.” 2014. Web. 06 Mar 2021.
Vancouver:
Gal A. The role of progesterone receptor in hypothalamic kisspeptin neurons. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/2142/49826.
Council of Science Editors:
Gal A. The role of progesterone receptor in hypothalamic kisspeptin neurons. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/49826
Texas Tech University
14.
Inigo, Melissa Mae.
Influence of the menstrual cycle phases on exercise-indeced bronchospasm and its effect on exercise performance.
Degree: Health Exercise and Sport Sciences, 2012, Texas Tech University
URL: http://hdl.handle.net/2346/45245
► The main purpose of the study was to determine the influence of the menstrual cycle phases on sex differences in EIB and exercise performance. Twenty-six…
(more)
▼ The main purpose of the study was to determine the influence of the menstrual cycle phases on sex differences in EIB and exercise performance. Twenty-six adults (18-35 yrs) with EIB were recruited in the study. Data analysis was performed in 7 women and 7 men who exhibited evidence of EIB (>10% drop in FEV1) during at least one visit. Women were tested once during the early follicular
phase and once during the mid-
luteal phase. Participants performed pulmonary function tests pre- and post- maximal treadmill exercise. Exercise ventilation at 95% maximal heart rate was similar between men and women across menstrual cycle phases (men: 67.80 ± 17.35%MVV vs. early follicular
phase: 78.59 ± 21.59%MVV vs. mid-
luteal phase: 83.23 ± 26.39%MVV, p > .05). No group differences were also found for time to exhaustion (men: 676.71 ± 96.9s vs. early follicular
phase: 621.57 ± 133.5s vs. mid-
luteal phase: 645.14 ± 91.0s; p > .05) and peak oxygen uptake phases (men: 47.62 ± 5.78 ml/kg/min vs. early follicular: 43.44 ± 5.57 ml/kg/min vs. mid-
luteal: 42.59 ± 3.90 ml/kg/min; p > .05). The peak percent drop in FEV1, FVC, PEF, and FEF25-75% following exercise were similar (p > .05) between men and women across menstrual cycle phases. Interestingly, post hoc analysis showed an inverse association between estimated airway size at rest and the post-exercise change in FEV1 (R2 = .58, p < .05). In conclusion, this study showed that menstrual cycle
phase does not influence EIB occurrence and severity or present sex differences in EIB. Furthermore, exercise performance was also not found to differ across the menstrual cycle. More studies are needed to investigate the relationship between airway size and EIB in women and men.
Advisors/Committee Members: Gonzales, Joaquin U. (Committee Chair), Gonzales, Joaquin U. (committee member), McComb, Jacalyn J. R. (committee member), Roncesvalles, Maria N. C. (committee member).
Subjects/Keywords: Asthma, exercise-induced; Menstrual cycle; Follicular phase; Luteal phase
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to Zotero / EndNote / Reference
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APA (6th Edition):
Inigo, M. M. (2012). Influence of the menstrual cycle phases on exercise-indeced bronchospasm and its effect on exercise performance. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/45245
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Inigo, Melissa Mae. “Influence of the menstrual cycle phases on exercise-indeced bronchospasm and its effect on exercise performance.” 2012. Thesis, Texas Tech University. Accessed March 06, 2021.
http://hdl.handle.net/2346/45245.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Inigo, Melissa Mae. “Influence of the menstrual cycle phases on exercise-indeced bronchospasm and its effect on exercise performance.” 2012. Web. 06 Mar 2021.
Vancouver:
Inigo MM. Influence of the menstrual cycle phases on exercise-indeced bronchospasm and its effect on exercise performance. [Internet] [Thesis]. Texas Tech University; 2012. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/2346/45245.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Inigo MM. Influence of the menstrual cycle phases on exercise-indeced bronchospasm and its effect on exercise performance. [Thesis]. Texas Tech University; 2012. Available from: http://hdl.handle.net/2346/45245
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Graaf, J.J. de.
Kisspeptin signaling and its role in canine reproduction: a basic understanding.
Degree: 2012, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/289517
► There are several central and peripheral mechanisms that influence the GnRH secretion. For a long time it has been a mystery how these mechanisms influence…
(more)
▼ There are several central and peripheral mechanisms that influence the
GnRH secretion. For a long time it has been a mystery how these mechanisms influence the
GnRH neurons. In recent years there have been exciting new discoveries in this field, especially with the discovery of
kisspeptin and its receptor.
Kisspeptin is a neuropeptide that seems to have an important role in the regulation of
GnRH neurons in the hypothalamus in various mammalian species. It is, among other things, supposed to be involved in the onset of puberty and in the mediation of the positive feedback exhibited by estrogens on the
GnRH neurons in females, which leads to the
GnRH/LH surge.
Although a lot of research has already been done, to our best knowledge,
kisspeptin and its receptor have not yet been researched in the
dog. Because the
kisspeptin signaling has been called a major gatekeeper of the gonadotropic axis, it would be interesting to discover its role in the canine reproduction. To lessen the amount of dogs that are yearly euthanized all over the world because of overpopulation, it is important to find a non-surgical method of contraception that prevents estrous cyclicity. The
kisspeptin signaling pathway could be a suitable target for developing an effective, safe, long-term or permanent non-surgical contraceptive for the bitch.
The aims of the present study were to identify the canine KISS1 and KISS1R genes and to characterize the amino acid sequences of canine
kisspeptin and the
kisspeptin receptor. The identification and characterization were done in silico. The results show that the KiSS1 and the KiSS1R gene are present at the canine genome and a large homology is found between the canine peptides and the peptides found in other mammalian species. This gives a basis to believe that
kisspeptin signaling is also important in
GnRH secretion in the
dog and that it may indeed be a suitable target for a non-surgical contraceptive.
Advisors/Committee Members: Albers-Wolthers, C.H.J..
Subjects/Keywords: Canine; dog; bitch; kisspeptin; reproduction
…and its receptor indicate a similar function of
the kisspeptin system in human and dog.
Fig… …of sap/exo mix
KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC… …seconds and
60° C for 30 seconds. The second step was also done at 55° C.
KISSPEPTIN SIGNALING… …KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING
9
RESULTS
IN… …preprokisspeptin. The amino acid
sequence of canine kisspeptin 10 differs only two amino acids from human…
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Export
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APA (6th Edition):
Graaf, J. J. d. (2012). Kisspeptin signaling and its role in canine reproduction: a basic understanding. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289517
Chicago Manual of Style (16th Edition):
Graaf, J J de. “Kisspeptin signaling and its role in canine reproduction: a basic understanding.” 2012. Masters Thesis, Universiteit Utrecht. Accessed March 06, 2021.
http://dspace.library.uu.nl:8080/handle/1874/289517.
MLA Handbook (7th Edition):
Graaf, J J de. “Kisspeptin signaling and its role in canine reproduction: a basic understanding.” 2012. Web. 06 Mar 2021.
Vancouver:
Graaf JJd. Kisspeptin signaling and its role in canine reproduction: a basic understanding. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Mar 06].
Available from: http://dspace.library.uu.nl:8080/handle/1874/289517.
Council of Science Editors:
Graaf JJd. Kisspeptin signaling and its role in canine reproduction: a basic understanding. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/289517
Michigan State University
16.
Fowler, Natasha.
Associations between ovarian hormones and emotional eating across the menstrual cycle : do ovulatory shifts in hormones matter?.
Degree: 2018, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:16306
► "Prior research suggests a substantial role for ovarian hormones in increased risk for binge eating and emotional eating during the mid-luteal phase of the menstrual…
(more)
▼ "Prior research suggests a substantial role for ovarian hormones in increased risk for binge eating and emotional eating during the mid-luteal phase of the menstrual cycle. However, past studies have not examined how and if pronounced hormonal changes that precede the mid-luteal phase (i.e., the dramatic decrease in estradiol and increase in progesterone during/after ovulation) also contribute to mid-luteal increases in binge-related symptoms. Past theories and studies of phenotypes strongly related to binge eating (e.g., depression) suggest that these pronounced hormonal changes may also play a role. This study examined this hypothesis in 390 female twins (aged 15-25 years) from the Michigan State University Twin Registry. Daily ratings of emotional eating (assessed with the Dutch Eating Behavior Questionnaire) and daily saliva samples of hormones were measured over 45 consecutive days. Results revealed no significant associations between pronounced changes in estradiol or progesterone across ovulation and emotional eating scores in the mid-luteal phase, even after controlling for BMI and negative affect and examining participants with clinical binge eating episodes. Taken together, data suggest that pronounced hormonal change across ovulation may play less of a role in emotional eating than changes in estradiol and progesterone that occur during the mid-luteal phase." – Page ii.
Online resource;
Advisors/Committee Members: Klump, Kelly L, Moser, Jason, Sisk, Cheryl.
Subjects/Keywords: Compulsive eating; Hormones, Sex; Menstrual cycle; Luteal phase; Clinical psychology
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CSE |
Export
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APA (6th Edition):
Fowler, N. (2018). Associations between ovarian hormones and emotional eating across the menstrual cycle : do ovulatory shifts in hormones matter?. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:16306
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fowler, Natasha. “Associations between ovarian hormones and emotional eating across the menstrual cycle : do ovulatory shifts in hormones matter?.” 2018. Thesis, Michigan State University. Accessed March 06, 2021.
http://etd.lib.msu.edu/islandora/object/etd:16306.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fowler, Natasha. “Associations between ovarian hormones and emotional eating across the menstrual cycle : do ovulatory shifts in hormones matter?.” 2018. Web. 06 Mar 2021.
Vancouver:
Fowler N. Associations between ovarian hormones and emotional eating across the menstrual cycle : do ovulatory shifts in hormones matter?. [Internet] [Thesis]. Michigan State University; 2018. [cited 2021 Mar 06].
Available from: http://etd.lib.msu.edu/islandora/object/etd:16306.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fowler N. Associations between ovarian hormones and emotional eating across the menstrual cycle : do ovulatory shifts in hormones matter?. [Thesis]. Michigan State University; 2018. Available from: http://etd.lib.msu.edu/islandora/object/etd:16306
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Otago
17.
Schafer, Danielle.
Functional Characterisation of Direct Neural Inputs to Arcuate Nucleus Kisspeptin Neurons in the Mouse
.
Degree: University of Otago
URL: http://hdl.handle.net/10523/7719
► The pulsatile pattern of gonadotropin release is critical for puberty and fertility. Kisspeptin neurons in the arcuate nucleus (ARN) are thought to play an important…
(more)
▼ The pulsatile pattern of gonadotropin release is critical for puberty and fertility.
Kisspeptin neurons in the arcuate nucleus (ARN) are thought to play an important role in generating pulsatile gonadotropin secretion. Previous studies have shown that
kisspeptin neurons within the ARN project to gonadotropin-releasing hormone (
GnRH) neurons and that the synchronous activation of
kisspeptin neurons generates pulsatile LH secretion in vivo. While studies indicated that ARN
kisspeptin neurons co-express and are regulated by dynorphin (DYN) and neurokinin B (NKB), the neurotransmitters that control these neurons have not yet been fully explored. Many different internal and external factors, such as changes in stress and metabolic state, modulate pulsatile gonadotropin secretion. I hypothesized the neurotransmitters mediating the effects of stress and metabolism within the brain may act directly on ARN
kisspeptin neurons to ultimately regulate pulsatile LH secretion. As such, I aimed to characterise the direct effects of several neurotransmitters upon the ARN
kisspeptin neurons. To identify effects, I recorded calcium activity from ARN
kisspeptin neurons in acute brain slices prepared from transgenic intact male and female mice expressing the calcium indicator GCaMP6f selectively in ARN
kisspeptin neurons. These experiments were conducted in the presence of a voltage-gated sodium channel blocker and ionotropic amino acid receptor antagonists to ensure that only direct effects were measured. The effects of neurotransmitters on intracellular calcium levels in ARN
kisspeptin neurons were determined by measuring changes in fluorescence within individual neurons.
In intact diestrous females, experiments demonstrated that NKB, corticotropin-releasing hormone, arginine vasopressin (AVP), oxytocin, noradrenaline (NA), serotonin (5-HT), dopamine, neuropeptide Y, vasoactive intestinal peptide (VIP), and pituitary adenylate cyclase-activating polypeptide (PACAP) all act directly on ARN
kisspeptin neurons to increase intracellular calcium levels, whereas DYN and beta-endorphin directly inhibit the stimulatory effects of NKB on these cells. Alpha-melanocyte-stimulating hormone (α-MSH) and
GnRH did not exhibit any direct effects on GCaMP6f fluorescence levels in ARN
kisspeptin neurons.
I also identified substantial regional and sex differences. Whereas, AVP, 5-HT, VIP, and PACAP all activated a significantly greater percentage of
kisspeptin neurons in caudal ARN slices, NA exerted its most potent effects in the middle ARN region. Furthermore, the effect of AVP, 5-HT, VIP, and NA were pronounced in intact females, but significantly reduced, or even absent, in intact males.
These results demonstrate that many of the neurotransmitters thought to be involved in mediating stress and metabolic actions within the brain also directly modulate the ARN
kisspeptin neurons. As such, these neurons may provide an integration point through which different modalities regulate pulsatile LH secretion.
Advisors/Committee Members: Herbison, Allan (advisor).
Subjects/Keywords: GnRH;
Kisspeptin;
Arcuate nucleus;
neuroendocrinology;
calcium imaging
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APA ·
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Export
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Manager
APA (6th Edition):
Schafer, D. (n.d.). Functional Characterisation of Direct Neural Inputs to Arcuate Nucleus Kisspeptin Neurons in the Mouse
. (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/7719
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Schafer, Danielle. “Functional Characterisation of Direct Neural Inputs to Arcuate Nucleus Kisspeptin Neurons in the Mouse
.” Masters Thesis, University of Otago. Accessed March 06, 2021.
http://hdl.handle.net/10523/7719.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Schafer, Danielle. “Functional Characterisation of Direct Neural Inputs to Arcuate Nucleus Kisspeptin Neurons in the Mouse
.” Web. 06 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Schafer D. Functional Characterisation of Direct Neural Inputs to Arcuate Nucleus Kisspeptin Neurons in the Mouse
. [Internet] [Masters thesis]. University of Otago; [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10523/7719.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Schafer D. Functional Characterisation of Direct Neural Inputs to Arcuate Nucleus Kisspeptin Neurons in the Mouse
. [Masters Thesis]. University of Otago; Available from: http://hdl.handle.net/10523/7719
Note: this citation may be lacking information needed for this citation format:
No year of publication.
18.
Dias, Rodrigo da Silva.
Estudo comparativo do fenótipo clínico de mulheres com transtorno afetivo bipolar em fase reprodutiva da vida com e sem piora pré-menstrual do humor.
Degree: PhD, Psiquiatria, 2010, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5142/tde-28052010-101220/
;
► O impacto da flutuação dos hormônios esteróides sobre o curso do Transtorno Afetivo Bipolar durante a vida reprodutiva das mulheres é pouco estudado. Encontramos ainda…
(more)
▼ O impacto da flutuação dos hormônios esteróides sobre o curso do Transtorno Afetivo Bipolar durante a vida reprodutiva das mulheres é pouco estudado. Encontramos ainda muitas lacunas no conhecimento quanto a sua apresentação clínica e as suas implicações na evolução do Transtorno Afetivo Bipolar, especialmente sua associação com a ciclagem rápida e o seu valor preditivo para recorrência. Métodos: Mulheres com Transtorno Afetivo Bipolar (tipos I, II ou sem outra especificação) participantes do Systematic Treatment Enhancement Program for Bipolar Disorder, com idade entre 16 e 40 anos, foram divididas em dois grupos: com e sem relato de exacerbação pré-menstrual do Transtorno Afetivo Bipolar na avaliação inicial do estudo. Estes grupos foram comparados em relação às características clínicas do Transtorno Afetivo Bipolar, de vida reprodutiva e tratamento na entrada do estudo. Longitudinalmente, foi comparado o tempo de recorrência entre as pacientes que iniciaram o estudo eutímicas utilizando análise de sobrevivência Kaplan Meier e a regressão de Cox. O número de episódios entre as pacientes que foram seguidas por um período de um ano também foi comparado. Resultados: Das 706 mulheres que completaram o questionário, 490 (69,4%) relataram exacerbação pré-menstrual. Na entrada do estudo, quando comparadas ao grupo sem exacerbação pré-menstrual, as mulheres com exacerbação pré-menstrual encontravam-se mais deprimidas, apresentavam mais comorbidades psiquiátricas, sintomas do humor com uso de contraceptivos hormonais, ciclos menstruais irregulares, e estavam recebendo menos tratamento farmacológico. Mulheres com exacerbação pré-menstrual também relataram mais episódios de humor durante o ano anterior e eram mais susceptíveis de apresentar ciclagem rápida neste mesmo período. Na avaliação prospectiva, entre as mulheres que iniciaram o estudo eutímicas (exacerbação pré-menstrual n = 66, sem exacerbação pré-menstrual n = 63), o grupo com exacerbação pré-menstrual teve um tempo de recaída mais rápido ao se incluir estados subsindrômicos associados a recaídas em mania, depressão ou estado misto. O tempo médio de recidiva de 50% da amostra foi de 4,5 meses para as mulheres com a exacerbação pré-menstrual, em comparação com 8,5 meses para as do grupo sem exacerbação pré-menstrual (p = 0,02). A exacerbação prémenstrual também foi um fator significativamente associado a maior gravidade dos sintomas depressivos e de elevação do humor entre as mulheres com um ano de seguimento. Conclusões: As mulheres com Transtorno Afetivo Bipolar que relataram exacerbação pré-menstrual apresentaram uma maior comorbidade psiquiátrica, maior número de episódios do humor no ano anterior e ciclagem rápida. A avaliação prospectiva mostrou uma taxa de recorrência maior, mais estados subsindrômicos e maior número de episódios, mas não ciclagem rápida, nas mulheres do grupo exacerbação pré-menstrual. Nossos resultados sugerem que a exacerbação pré-menstrual pode ser considerada um marcador clínico preditor de um fenótipo clínico mais complexo e associado a…
Advisors/Committee Members: Lafer, Beny.
Subjects/Keywords: Bipolar disorder; Clinical evolution; Evolução clínica; Fase luteal; Luteal phase; Menstruação; Menstruation; Mulheres; Transtorno bipolar; Women
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APA ·
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Manager
APA (6th Edition):
Dias, R. d. S. (2010). Estudo comparativo do fenótipo clínico de mulheres com transtorno afetivo bipolar em fase reprodutiva da vida com e sem piora pré-menstrual do humor. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5142/tde-28052010-101220/ ;
Chicago Manual of Style (16th Edition):
Dias, Rodrigo da Silva. “Estudo comparativo do fenótipo clínico de mulheres com transtorno afetivo bipolar em fase reprodutiva da vida com e sem piora pré-menstrual do humor.” 2010. Doctoral Dissertation, University of São Paulo. Accessed March 06, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5142/tde-28052010-101220/ ;.
MLA Handbook (7th Edition):
Dias, Rodrigo da Silva. “Estudo comparativo do fenótipo clínico de mulheres com transtorno afetivo bipolar em fase reprodutiva da vida com e sem piora pré-menstrual do humor.” 2010. Web. 06 Mar 2021.
Vancouver:
Dias RdS. Estudo comparativo do fenótipo clínico de mulheres com transtorno afetivo bipolar em fase reprodutiva da vida com e sem piora pré-menstrual do humor. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Mar 06].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5142/tde-28052010-101220/ ;.
Council of Science Editors:
Dias RdS. Estudo comparativo do fenótipo clínico de mulheres com transtorno afetivo bipolar em fase reprodutiva da vida com e sem piora pré-menstrual do humor. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/5/5142/tde-28052010-101220/ ;
19.
Pousias, Serafeim.
Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών.
Degree: 2019, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας
URL: http://hdl.handle.net/10442/hedi/46215
► Ganirelix is a GnRH antagonist, which is widely used in Assisted Reproduction Technology (ART), for the prevention of premature LH peaks. Kisspeptin is a neuropeptide,…
(more)
▼ Ganirelix is a GnRH antagonist, which is widely used in Assisted Reproduction Technology (ART), for the prevention of premature LH peaks. Kisspeptin is a neuropeptide, which is secreted from the hypothalamus, and may have a crucial role on the onset of puberty in humans and on ovulation in women. The aim of this study was to examine the effect of Ganirelix on the secretion of LH and on follicle maturation in healthy women. Patients and measurements: Ten healthy female volunteers were studied during two menstrual cycles, i.e. cycle 1 (control, spontaneous cycle) and cycle 2 (Ganirelix). In both cycles, blood samples were taken daily starting on cycle day 2 until ovulation occurred on cycle 1 and transvaginal ultrasound was performed on cycle day 8 and 10 and then on a daily basis until ovulation occurred. During cycle 2, all women were given S.C. injections of the GnRH antagonist Ganirelix at the dose of 0,25 mg/day, starting on cycle day 2 until the day of the endogenous LH surge in cycle 1. In all blood samples, FSH, LH, Estradiol, Progesterone and Kisspeptin levels were measured. Results: During treatment with Ganirelix FSH levels remained stable and similar to those in cycle 1. LH levels were reduced in cycle 2 as compared to cycle 1 on days 3, 4, 7 and 9 (P<0,05), with no significant within-cycle variation. Estradiol levels until day 10 did not differ significantly between the two cycles. After day 10, no follicle maturation and no substantial increase in serum estradiol levels were observed in the antagonist cycle (cycle 2), in contrast to cycle 1. In cycle 1, ovulation was observed in 9 women. In cycle 2, one volunteer ovulated, despite the treatment with Ganirelix, and in 8 women ovulation was postponed for a week. Serum Kisspeptin levels were similar in both cycles, except on days 5 and 6 (P<0,05) in which they were higher in cycle 2. There was no significant within-cycle variation in Kisspeptin levels during the follicular phase. No correlation was found between serum FSH, estradiol and progesterone levels and those of Kisspeptin. However, Kisspeptin levels showed a positive correlation with those of LH on cycle days 2 (r=0,89, Ρ<0,01) and 3 (r=0,79, Ρ<0,05). Conclusions: This study shows for the first time that Ganirelix interferes with the selection of the dominant follicle. Since circulating gonadotrophin levels did not change substantially, it is suggested that Ganirelix affects the process of follicle selection by acting on the ovary. This, however, needs to be further investigated. Regarding the role of Kisspeptin in Ganirelix-suppressed gonadotrophin secretion further studies are required.
Ο Ganirelix είναι ένας GnRH ανταγωνιστής που χρησιμοποιείται για να εμποδίσει το πρώιμο κύμα της LH κατά τη διέγερση των ωοθηκών. Η κισσπεπτίνη είναι ένα νευροπεπτίδιο το οποίο εκκρίνεται από την υποθάλαμο και πιθανότατα διαδραματίζει κύριο ρόλο στον άξονα Υποθάλαμος-Υπόφυση-Γονάδων. Σκοπός της παρούσας διδακτορικής διατριβής ήταν να μελετήσει την επίδραση του Ganirelix στην έκκριση των γοναδοτροφινών και στην…
Subjects/Keywords: GnRH ανταγωνιστής; Ωοθυλακική φάση; Ωοθήκη; Ωοθυλάκιο; Ganirelix; GnRH antagonist; Ovary; Follicle; Follicular phase; Ganirelix
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APA (6th Edition):
Pousias, S. (2019). Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/46215
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pousias, Serafeim. “Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών.” 2019. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed March 06, 2021.
http://hdl.handle.net/10442/hedi/46215.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pousias, Serafeim. “Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών.” 2019. Web. 06 Mar 2021.
Vancouver:
Pousias S. Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2019. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10442/hedi/46215.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pousias S. Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2019. Available from: http://hdl.handle.net/10442/hedi/46215
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Decourt, Caroline.
Le kisspeptide : nouvelle molécule pour la maîtrise du cycle chez la jument ? : No title available.
Degree: Docteur es, Sciences de la vie, 2012, Université François-Rabelais de Tours
URL: http://www.theses.fr/2012TOUR4046
► Les kisspeptides (KP), puissants sécrétagogues de la GnRH, agissent par l'intermédiaire du récepteur couplé aux protéines G, le GPR54. Une perfusion i.v. de KP est…
(more)
▼ Les kisspeptides (KP), puissants sécrétagogues de la GnRH, agissent par l'intermédiaire du récepteur couplé aux protéines G, le GPR54. Une perfusion i.v. de KP est non seulement capable de synchroniser les ovulations chez la brebis cyclique, mais également d’induire un cycle chez la brebis en anoestrus. L'objectif de ma thèse a été d'étudier la neuroanatomie du système KP / GnRH, et de définir des applications potentielles du KP dans le contrôle du cycle chez la jument cyclique et en anoestrus. Nos résultats ont permis de montrer que 1) le KP interagit avec le système GnRH au niveau hypothalamique, 2) une perfusion i.v. de KP en début de phase folliculaire conduit à une stimulation - quoique transitoire – de la sécrétion des gonadotropines, 3) une capacité limitée pour reconstituer le stock en GnRH et / ou LH / FSH pourrait expliquer la nature transitoire de la stimulation par le KP, 4) une perfusion de KP pendant 3 jours, depuis le début de la phase folliculaire, tend à avancer le pic péri-ovulatoire de LH et l'ovulation, 5) une perfusion plus longue (soit plus de 3 jours) de KP, pendant toute la phase folliculaire, n’induit pas une ovulation plus précoce, 6) pendant la saison d'anoestrus, une perfusion de KP pendant 3 jours stimule la sécrétion de LH, avec une intensité néanmoins inférieure à celle observée avec une perfusion de GnRH. En conclusion, bien que l'utilisation potentielle du KP pour synchroniser ou induire l'ovulation pendant la saison de reproduction semble discutable, sa capacité à induire des cycles durant la période d'anoestrus présente un intérêt et mérite des études plus approfondies.
Kisspeptins (KP) are very potent secretagogues of GnRH which act through the G protein-coupled receptor GPR54. An i.v. infusion of KP is not only able to synchronize ovulations of cyclic ewes, but also to induce cycle in anoestrus ewes. The aim of my thesis was to investigate the neuroanatomy of the KP / GnRH system, and define potential applications for KP in the control of the estrus cycle in the cyclic and anoestrus mares. Our results showed that 1) KP interact with GnRH systems at the hypothalamic level, 2) an i.v. infusion of KP during early follicular phase leads to an enhanced - albeit transient - secretion of gonadotropins, 3) a limited ability to replenish GnRH and/or LH/FSH stores might explain the transient nature of the KP stimulation, 4) an infusion of KP for 3 days, since early follicular phase, modestly advances the periovulatory LH surge and the ovulation, 5) a longer (i.e. more than 3 days) infusion of KP during all the follicular phase does not lead to precocious ovulation, 6) during the anoestrus season, an infusion of KP for 3 days heightens LH secretion, with however a lower intensity to that observed with an infusion of GnRH. In conclusion, albeit the potential use of KP to synchronize or induce ovulation during the breeding season appears questionable, its ability to induce cycles during the anoestrus period is of interest and warrants further investigation.
Advisors/Committee Members: Caraty, Alain (thesis director), Gaillot-Briant, Christine (thesis director).
Subjects/Keywords: Kisspeptide; Jument; Gonadotropines; Phase folliculaire; Anœstrus; Kisspeptin; Mare; Gonadotropins; Follicular phase; Anœstrus
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APA (6th Edition):
Decourt, C. (2012). Le kisspeptide : nouvelle molécule pour la maîtrise du cycle chez la jument ? : No title available. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2012TOUR4046
Chicago Manual of Style (16th Edition):
Decourt, Caroline. “Le kisspeptide : nouvelle molécule pour la maîtrise du cycle chez la jument ? : No title available.” 2012. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 06, 2021.
http://www.theses.fr/2012TOUR4046.
MLA Handbook (7th Edition):
Decourt, Caroline. “Le kisspeptide : nouvelle molécule pour la maîtrise du cycle chez la jument ? : No title available.” 2012. Web. 06 Mar 2021.
Vancouver:
Decourt C. Le kisspeptide : nouvelle molécule pour la maîtrise du cycle chez la jument ? : No title available. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2012. [cited 2021 Mar 06].
Available from: http://www.theses.fr/2012TOUR4046.
Council of Science Editors:
Decourt C. Le kisspeptide : nouvelle molécule pour la maîtrise du cycle chez la jument ? : No title available. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2012. Available from: http://www.theses.fr/2012TOUR4046
Oregon State University
21.
Jacobs, Dakota.
Effect of PFAS Exposure on Reproduction : a Comparative Investigation between Kisspeptin-secreting AVPV and Arcuate Nuclei.
Degree: MS, Toxicology, 2016, Oregon State University
URL: http://hdl.handle.net/1957/59888
► Ovulation requires preovulatory surges of gonadotropin-releasing hormone (GnRH) from preoptic hypothalamic neurons, initiated by elevated ovarian estradiol (E₂). Rising estradiol activates a subset of sexually…
(more)
▼ Ovulation requires preovulatory surges of gonadotropin-releasing hormone (
GnRH) from preoptic hypothalamic neurons, initiated by elevated ovarian estradiol (E₂). Rising estradiol activates a subset of sexually dimorphic
kisspeptin (Kiss-1) neurons in the female, located in the anteroventral periventricular nuclei (AVPV). Conversely, estradiol negative feedback on
GnRH secretion is mediated by a neuroanatomically separate population of Kiss-1 neurons in the arcuate nuclei.
Kisspeptin stimulates
GnRH expression and secretion in vivo, and the development of this system is critical for the initiation of puberty. To elucidate how phenotypically similar Kiss-1 neuronal populations react differentially to estradiol exposure, we have generated two immortalized Kiss-1 cell lines from kiss1-GFP post-pubertal female mice. These cell models recapitulate in vivo differential responsiveness to estradiol, with KTaV-3 (AVPV-derived) demonstrating ~6-fold increases in kiss1 expression under higher estradiol doses (5pM – 50pM E₂), while kiss1 expression in KTaR-1 cells is suppressed up to 80% under lower E2 concentrations (2pM – 10pM). We probed temporal patterns of kiss1 and core clock gene expression in these lines in response to estradiol, and found distinct antiphasic patterns of bmal1 and per2 in KTaV-3 cells irrespective of estradiol exposure. Treatment of KTaV-3 cells with 25pM E₂, however, elicited distinct patterns of kiss1 expression over time in contrast to vehicle, suggesting differential coupling of intracellular oscillators to kiss1 transcriptional activity in the presence of estradiol. Further, we have found that expression alterations between nuclear receptor ERα and ERβ genes, esr1 and esr2, respectively, fluctuate divergently between these lines. We have implicated that the peaks of kiss1 expression demonstrated by the KTaV-3 lines may be mediated by both classical and non-classical estrogen signaling. In addition, we provide evidence that the negative regulation of kiss1 expression in KTaR-1 cells may be a function of mutual antagonism due to overabundance of contemporaneously expressed esr1 and esr2 genes that is not observed in KTaV-3 lines. Lastly, we explored the impact of an endocrine disrupting class of perfluorinated alkyl substances (PFASs) on these neurons, with preliminary results illustrating kiss1, esr1, and esr2 transcriptional activation and/or repression at relevant doses of perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), and perfluorohexanoic acid (PFHxA) in the two lines. At extremely low doses of PFOA, typical estrogenic demonstrations of kiss1 expression are presented by KTaV-3 and KTaR-1 neurons. However at the same dose of PFOS the expression modulation of kiss1 gated by estrogen signaling in this hypothalamic populations is flipped. This implicates that a sufficient exposure to these ubiquitous chemicals can have a potent effect on neuronal expression profiles for estrogen sensitive genes that is a complex function of dose, particular PFAS, and tissue type. Ongoing…
Advisors/Committee Members: Chappell, Patrick (advisor), Buermeyer, Andrew (committee member).
Subjects/Keywords: Kisspeptin; Kisspeptin neurons
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APA ·
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APA (6th Edition):
Jacobs, D. (2016). Effect of PFAS Exposure on Reproduction : a Comparative Investigation between Kisspeptin-secreting AVPV and Arcuate Nuclei. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/59888
Chicago Manual of Style (16th Edition):
Jacobs, Dakota. “Effect of PFAS Exposure on Reproduction : a Comparative Investigation between Kisspeptin-secreting AVPV and Arcuate Nuclei.” 2016. Masters Thesis, Oregon State University. Accessed March 06, 2021.
http://hdl.handle.net/1957/59888.
MLA Handbook (7th Edition):
Jacobs, Dakota. “Effect of PFAS Exposure on Reproduction : a Comparative Investigation between Kisspeptin-secreting AVPV and Arcuate Nuclei.” 2016. Web. 06 Mar 2021.
Vancouver:
Jacobs D. Effect of PFAS Exposure on Reproduction : a Comparative Investigation between Kisspeptin-secreting AVPV and Arcuate Nuclei. [Internet] [Masters thesis]. Oregon State University; 2016. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1957/59888.
Council of Science Editors:
Jacobs D. Effect of PFAS Exposure on Reproduction : a Comparative Investigation between Kisspeptin-secreting AVPV and Arcuate Nuclei. [Masters Thesis]. Oregon State University; 2016. Available from: http://hdl.handle.net/1957/59888
22.
Vosges, Mélanie.
Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.
Degree: Docteur es, Sciences de la vie, 2010, Université François-Rabelais de Tours
URL: http://www.theses.fr/2010TOUR4035
► A ce jour, les effets des perturbateurs endocriniens (PE) sur les circuits neuroendocrines contrôlant la fonction de reproduction ont fait l’objet de très peu de…
(more)
▼ A ce jour, les effets des perturbateurs endocriniens (PE) sur les circuits neuroendocrines contrôlant la fonction de reproduction ont fait l’objet de très peu de travaux. Chez les vertébrés, l’élément majeur du contrôle central de la fonction de reproduction est la GnRH (Gonadotropin-Releasing Hormone). Le développement et l’activité des neurones à GnRH sont finement régulés, notamment par les hormones stéroïdes, ce qui les rend potentiellement sensibles aux PE. L’objectif de ce travail était d'étudier les effets neuroendocrines des xéno-œstrogènes chez le poisson zèbre (Danio rerio). Nous montrons que le 17α-éthinylestradiol (EE2) et le nonylphénol (NP) perturbent l'ontogenèse du système à GnRH au cours du développement précoce. De plus, nous démontrons que ces effets impliquent des récepteurs des œstrogènes. Parallèlement, nous mettons en évidence l’effet inducteur de l’EE2 et du NP sur l'expression de l’aromatase cérébrale, l’enzyme de synthèse des œstrogènes. L’ensemble de ces données souligne la nécessité de considérer les réseaux neuroendocrines comme des variables critiques et sensibles dans le domaine de la perturbation endocrinienne.
Until now, studies dedicated to the actions of endocrine disrupting chemicals (EDCs) on the reproductive axis have focused on the gonads and peripheral organs leaving virtually unexplored their actions on neuroendocrine circuits controlling reproduction. In vertebrates, gonadotropin-releasing hormone (GnRH) is the key factor controlling the activity of the reproductive axis. The development and functioning of GnRH neurons are finely tuned, notably by sex steroids, making these neurons potential targets of EDCs. The aim of this work was to explore the neuroendocrine effects of xenoestrogens in the zebrafish (Danio rerio). We show that 17α-ethinylestradiol (EE2) and nonylphenol (NP) disrupts the ontogeny of GnRH system during zebrafish early life stage. Moreover, we demonstrate that these effects involve functional estrogens receptors. In parallel, we report the inducing effects of EE2 and NP on the expression of brain aromatase protein, the enzyme responsible for estrogen biosynthesis. Altogether, these results highlight the need to consider neuroendocrine networks as critical and sensitive endpoints in the field of endocrine disruption.
Advisors/Committee Members: Combarnous, Yves (thesis director).
Subjects/Keywords: GnRH
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APA (6th Edition):
Vosges, M. (2010). Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2010TOUR4035
Chicago Manual of Style (16th Edition):
Vosges, Mélanie. “Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.” 2010. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 06, 2021.
http://www.theses.fr/2010TOUR4035.
MLA Handbook (7th Edition):
Vosges, Mélanie. “Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.” 2010. Web. 06 Mar 2021.
Vancouver:
Vosges M. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2010. [cited 2021 Mar 06].
Available from: http://www.theses.fr/2010TOUR4035.
Council of Science Editors:
Vosges M. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2010. Available from: http://www.theses.fr/2010TOUR4035
23.
Friedman, Zoey.
Regulation of Kisspeptin-expressing Neurons and Stimulatory Mode of Action of Kisspeptin in Immortalized Hypothalamic Cell Models.
Degree: 2013, University of Toronto
URL: http://hdl.handle.net/1807/67247
► Fertility is a highly regulated process dependent on the orchestration of hypothalamic neuropeptides and peripheral hormones, which converge on GnRH neurons in the HPG axis.…
(more)
▼ Fertility is a highly regulated process dependent on the orchestration of hypothalamic neuropeptides and peripheral hormones, which converge on GnRH neurons in the HPG axis. Kisspeptin and its receptor, Gpr54 have emerged as fundamental gatekeepers of reproduction, acting upstream of GnRH neurons. Kiss neurons have been found to express estrogen receptors, Gpr54 and the GnIH receptor, Gpr147. We have generated immortalized, murine hypothalamic cell lines to investigate mechanisms of gene transcription in kisspeptin-expressing neuronal cell models. We show using qRT-PCR and western blot analyses that kisspeptin-expressing neuronal models are targets for gonadal steroids and hypothalamic neuropeptides. Further, using a novel GnRH-secreting cell line, we report that GnRH neurons expressing Gpr54 and Gpr47 are stimulated by kisspeptin, although suppressed by a kisspeptin/GnIH cotreatnent. Overall, these studies expand our knowledge of the regulation of Kiss neurons by estradiol, kisspeptin and GnIH, improving our understanding of kisspeptin as a modulator of GnRH neurons.
MAST
Advisors/Committee Members: Belsham, Denise, Physiology.
Subjects/Keywords: Kisspeptin; Hypothalamus; Neuron; GnRH; Estradiol; GnIH; Neuropeptide; 0719
…85
Figure 5.2. Kisspeptin induces c-Fos mRNA expression in the
mHypoA-GnRH/GFP neurons… …87
Figure 5.3. Kisspeptin-mediated regulation of GnRH mRNA expression in mHypoAGnRH/GFP… …kisspeptin on hypothalamic GnRH neurons downstream. To complete these studies, we
have utilized… …the pituitary, kisspeptin stimulates release of GnRH
into the portal system, which then… …suggesting that kisspeptin also acts
indirectly on GnRH neurons to modulate GnRH release via its…
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APA ·
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APA (6th Edition):
Friedman, Z. (2013). Regulation of Kisspeptin-expressing Neurons and Stimulatory Mode of Action of Kisspeptin in Immortalized Hypothalamic Cell Models. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/67247
Chicago Manual of Style (16th Edition):
Friedman, Zoey. “Regulation of Kisspeptin-expressing Neurons and Stimulatory Mode of Action of Kisspeptin in Immortalized Hypothalamic Cell Models.” 2013. Masters Thesis, University of Toronto. Accessed March 06, 2021.
http://hdl.handle.net/1807/67247.
MLA Handbook (7th Edition):
Friedman, Zoey. “Regulation of Kisspeptin-expressing Neurons and Stimulatory Mode of Action of Kisspeptin in Immortalized Hypothalamic Cell Models.” 2013. Web. 06 Mar 2021.
Vancouver:
Friedman Z. Regulation of Kisspeptin-expressing Neurons and Stimulatory Mode of Action of Kisspeptin in Immortalized Hypothalamic Cell Models. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1807/67247.
Council of Science Editors:
Friedman Z. Regulation of Kisspeptin-expressing Neurons and Stimulatory Mode of Action of Kisspeptin in Immortalized Hypothalamic Cell Models. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/67247
24.
Merkley, Christina M.
The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System.
Degree: 2013, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/1386
► The gonadotropin-releasing hormone (GnRH) neurons in the hypothalamus represent the final common output in the central control of reproduction. GnRH secretion is modulated indirectly by…
(more)
▼ The gonadotropin-releasing hormone (GnRH) neurons in the hypothalamus represent the final common output in the central control of reproduction. GnRH secretion is modulated indirectly by steroid feedback action of gonadal steroids on afferent interneurons. In recent years, the neuropeptide, kisspeptin, has emerged as a key mediator of steroid feedback onto GnRH neurons and the reproductive system. Kisspeptin neurons located in the preoptic area (POA) mediate estradiol (E2) positive feedback leading to the preovulatory surge, and kisspeptin cells in the arcuate nucleus (ARC) have been shown to mediate the negative feedback actions of E2 on pulsatile GnRH secretion. ARC kisspeptin neurons are distinguished from those in POA by their extensive reciprocal connections with each other, and their co-expression of two other neuropeptides, neurokinin B (NKB) and dynorphin. Because of this co-expression, the ARC kisspeptin neurons have been termed “KNDy” (Kisspeptin/NKB/Dynorphin) cells. Using sheep and mice as neuroendocrine models, the goal of this dissertation was to investigate the functional organization of KNDy cells and their projections to GnRH neurons, in order to gain insight into their role as mediators of steroid feedback. First, we showed that, contrary to expectations based on rodent work, KNDy neurons in the sheep are activated during both positive and negative feedback of E2 suggesting that they are common mediators of both GnRH pulses and the GnRH surge. Next, using KNDy cell peptides as markers, we showed that KNDy neurons in the sheep send direct neuronal projections to a majority of GnRH cell bodies spread over multiple regions, as well as input to GnRH terminals in the median eminence. We then explored the potential plasticity of synaptic inputs onto KNDy and GnRH neurons across the ovine estrous cycle, and found a significant increase in the total number of inputs to KNDy neurons, as well as KNDy inputs to GnRH cells, at the time of the preovulatory GnRH surge. Performing similar experiments in transgenic mice, we tested the hypothesis that plasticity in these inputs is regulated, in part, by E2. Finally, we began to investigate the possibility that NKB released by KNDy cells might play a functional role in the GnRH surge, by examining internalization of the NKB receptor, NK3R, across the estrous cycle. Taken together, these findings provided novel information on the roles of kisspeptin and KNDy neurons in steroid feedback control of GnRH secretion, and set the stage for future experiments to explore the mechanisms for steroid feedback in individual neurons, the functional role of synaptic plasticity in this system, and the role of KNDy peptides in regulating the reproductive neuroendocrine system across the estrous cycle.
Subjects/Keywords: Kisspeptin; reproduction; KNDy; neuroendocrinology; GnRH; plasticity; Endocrine System; Neurosciences
…phase and
are elevated throughout luteal phase, where they act to suppress GnRH/LH pulse… …of interaction between kisspeptin and GnRH neurons
46
2.5.
Heterogeneity among… …RESULTS
viii
4.3.1.
Direct inputs from KNDy (Kisspeptin/Dynorphin) neurons to GnRH… …INPUTS TO KISSPEPTIN
AND GNRH NEURONS DURING THE PREOVULATORY LH SURGE IN
THE EWE
109
5.1… …x28;pg/min) and LH (ng/ml) release during luteal and in late
follicular phase…
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APA ·
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APA (6th Edition):
Merkley, C. M. (2013). The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/1386
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Merkley, Christina M. “The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System.” 2013. Thesis, University of Western Ontario. Accessed March 06, 2021.
https://ir.lib.uwo.ca/etd/1386.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Merkley, Christina M. “The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System.” 2013. Web. 06 Mar 2021.
Vancouver:
Merkley CM. The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System. [Internet] [Thesis]. University of Western Ontario; 2013. [cited 2021 Mar 06].
Available from: https://ir.lib.uwo.ca/etd/1386.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Merkley CM. The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System. [Thesis]. University of Western Ontario; 2013. Available from: https://ir.lib.uwo.ca/etd/1386
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
25.
松木, 千紗.
Associations between brain-derived neurotrophic factor and estradiol in women's saliva. : 女性の唾液における脳由来神経栄養因子とエストラジオールの関連.
Degree: 博士（歯学）, 2016, Kanagawa Dental University / 神奈川歯科大学
URL: http://id.nii.ac.jp/1421/00000901/
► The aim of this study was to assess the relationship between brain-derived neurotrophic factor (BDNF) and sex hormones (estradiol [E2] and progesterone), using saliva samples…
(more)
▼ The aim of this study was to assess the relationship between brain-derived neurotrophic factor (BDNF) and sex hormones (estradiol [E2] and progesterone), using saliva samples obtained from healthy women.
Forty female dental hygienist students were divided into groups according to being in the follicular phase or luteal phase. Saliva BDNF, E2, and progesterone levels were measured using a sandwich ELISA system. The correlation between these factors was analyzed using Spearman's index, and fluctuations of these levels in the whole menstrual cycle were investigated classifying the subjects by every 4 days according to the phase of their menstrual cycle.
Saliva BDNF variations strongly correlated with saliva E2 levels in the follicular phase (r = 0.721, p = 0.000) and luteal phase (r = 0.770, p = 0.000). The correlation coefficient showing the relationship between progesterone and BDNF levels in the luteal phase (r = 0.371, p = 0.157) was lower than that in the follicular phase (r = 0.631, p = 0.001). Moreover, the fluctuation of BDNF levels in the menstrual cycle followed a similar pattern to that of E2.
We found that saliva BDNF and E2 levels were closely related in healthy young women. In particular, for first time, that correlation was investigated throughout the menstrual cycle. Monitoring of saliva BDNF may yield insight into women's reproductive and mental health.
2013
Subjects/Keywords: Adult; Brain-Derived Neurotrophic Factor; Estradiol; Female; Follicular Phase; Humans; Luteal Phase; Monitoring, Physiologic; Progesterone; Saliva; Young Adult
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APA (6th Edition):
松木, . (2016). Associations between brain-derived neurotrophic factor and estradiol in women's saliva. : 女性の唾液における脳由来神経栄養因子とエストラジオールの関連. (Thesis). Kanagawa Dental University / 神奈川歯科大学. Retrieved from http://id.nii.ac.jp/1421/00000901/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
松木, 千紗. “Associations between brain-derived neurotrophic factor and estradiol in women's saliva. : 女性の唾液における脳由来神経栄養因子とエストラジオールの関連.” 2016. Thesis, Kanagawa Dental University / 神奈川歯科大学. Accessed March 06, 2021.
http://id.nii.ac.jp/1421/00000901/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
松木, 千紗. “Associations between brain-derived neurotrophic factor and estradiol in women's saliva. : 女性の唾液における脳由来神経栄養因子とエストラジオールの関連.” 2016. Web. 06 Mar 2021.
Vancouver:
松木 . Associations between brain-derived neurotrophic factor and estradiol in women's saliva. : 女性の唾液における脳由来神経栄養因子とエストラジオールの関連. [Internet] [Thesis]. Kanagawa Dental University / 神奈川歯科大学; 2016. [cited 2021 Mar 06].
Available from: http://id.nii.ac.jp/1421/00000901/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
松木 . Associations between brain-derived neurotrophic factor and estradiol in women's saliva. : 女性の唾液における脳由来神経栄養因子とエストラジオールの関連. [Thesis]. Kanagawa Dental University / 神奈川歯科大学; 2016. Available from: http://id.nii.ac.jp/1421/00000901/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Linnaeus University
26.
Fäldt, Anna.
Menstruationscykelns påverkan på kvinnors prestation : - En litteraturstudie.
Degree: Sport Science, 2020, Linnaeus University
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-95141
► Bakgrund: De hormonella förändringarna som sker under menstruationscykeln bidrar till variationer i olika fysiologiska processer som påverkar både kvinnors mående och prestation. Det är…
(more)
▼ Bakgrund: De hormonella förändringarna som sker under menstruationscykeln bidrar till variationer i olika fysiologiska processer som påverkar både kvinnors mående och prestation. Det är inte enbart det välstuderade premenstruella syndromet innan blödning som påverkar utan variationer i könshormonerna under hela menstruationscykeln påverkar olika fysiologiska processer. Forskning om optimering av styrke- och konditionsträning utgår ofta från studier med manliga deltagare som har testosteron som uppbyggande hormon. Forskning på kvinnor visar att de hormonella förändringarna under menstruationscykeln bidrar till variation i metabolism, styrka, inflammation, vätskebalans, kroppstemperatur och risk för skador. Syfte: Litteraturstudiens syfte var att undersöka hur menstruationscykeln påverkar prestationsförmågan hos idrottande kvinnor. Metod: En litteratursökning genomfördes i databaserna PubMed och Sport Discus. Även en utökad sökning och en sekundärsökning utfördes. Totalt inkluderades tio studier. Resultat: Fem studier undersökte skillnader i muskelstyrka mellan follikelfasen (första blödningsdag fram till ägglossning) och lutealfasen (ägglossning fram till dagen innan nästa blödning). Fyra av studierna visade en ökad muskelstyrka och större muskeltillväxt under follikelfasen och en av studierna visade ingen skillnad mellan faserna. Fem studier undersökte syreupptagningsförmåga mellan follikel- och lutealfas. Två av studierna visade en högre syreupptagningsförmåga under lutealfasen, och tre av studierna visade inga skillnader mellan faserna. Slutsats: Studierna kring muskelstyrka indikerar att effekten av styrketräning är störst under follikelfasen, varemot flera studier behövs för att kunna utreda hur menstruationscykeln påverkar kvinnors syreupptagningsförmåga.
Background: The hormonal changes that occur during the menstrual cycle contribute to variations in different physiological processors that affect both women's sensation and performance. It is not only the well-studied premenstrual syndrome of bleeding that affects women but the continuous variation in the steoridhormones throughout the menstrual cycle affect several physiological functions. Research on optimization strength and fitness training is often based on studies with male participants who have testosterone as the dominant anabolic hormone. Research on women shows that the hormonal changes during the menstrual cycle contribute to variation in metabolism, strength, inflammation, fluid balance, body temperature and risk of injury. Purpose: This literature study purpose was to investigate how menstrual cycles affect the performance of athletic women. Method: A literature search was carried out using the PubMed and Sport Discus databases. An extended search and a secondary search were also performed and a total of ten studies were included. Results: Five studies examined differences in muscle…
Subjects/Keywords: Female athletes; follicle phase; hormones; luteal phase; menstruation; performance; Follikelfas; hormoner; kvinnliga atleter; lutealfas; menstruation; prestation; Sport and Fitness Sciences; Idrottsvetenskap
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APA (6th Edition):
Fäldt, A. (2020). Menstruationscykelns påverkan på kvinnors prestation : - En litteraturstudie. (Thesis). Linnaeus University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-95141
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fäldt, Anna. “Menstruationscykelns påverkan på kvinnors prestation : - En litteraturstudie.” 2020. Thesis, Linnaeus University. Accessed March 06, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-95141.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fäldt, Anna. “Menstruationscykelns påverkan på kvinnors prestation : - En litteraturstudie.” 2020. Web. 06 Mar 2021.
Vancouver:
Fäldt A. Menstruationscykelns påverkan på kvinnors prestation : - En litteraturstudie. [Internet] [Thesis]. Linnaeus University; 2020. [cited 2021 Mar 06].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-95141.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fäldt A. Menstruationscykelns påverkan på kvinnors prestation : - En litteraturstudie. [Thesis]. Linnaeus University; 2020. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-95141
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Tech
27.
Hess, Milan B.
The Effects of Prostaglandin F2a, Oxytocin and Gonadotropin Releasing Hormone on Ejaculate Characteristics in the Dog.
Degree: MS, Veterinary Medical Sciences, 2002, Virginia Tech
URL: http://hdl.handle.net/10919/31135
► Prostaglandin F2a (PGF2a), oxytocin and gonadotropin releasing hormone (GnRH) have been used in bulls, rams, boars, stallions or rodents to increase sperm numbers in the…
(more)
▼ Prostaglandin F2a (PGF2a), oxytocin and gonadotropin releasing hormone (
GnRH) have been used in bulls, rams, boars, stallions or rodents to increase sperm numbers in the ejaculate. Improving sperm quantity in the canine ejaculate would benefit all assisted reproductive techniques used in this species. The purpose of the present study was to evaluate the effects of PGF2a, oxytocin and
GnRH on canine ejaculate characteristics.
Eight, mature, medium size (25-30 kg), mixed breed dogs were randomly assigned to one of four treatment groups (N=2 dogs each); each group received one treatment per week for four weeks. Treatments were assigned based on a Latin Square design. A two-week training period was used to acclimate the dogs to manual semen collection. Treatments were 0.1 mg/kg PGF2a 15 minutes prior to collection, 2.5 units/
dog oxytocin 10 minutes prior to collection, 50 mg/
dog GnRH 60 minutes prior to collection, or 1.0 ml of saline 30 minutes prior to collection. An evaluator that was blinded to treatment analyzed ejaculate characteristics. Samples were evaluated for semen volume, concentration of spermatozoa per milliliter, motility, morphology, total sperm number and total morphologically normal motile sperm number (TNMS). In addition, a subjective ease of collection score was assigned following each collection (Scale 1-9, 1 being easiest to manually ejaculate).
Semen concentration, motility and morphology were not different between treatments. Semen volume was greater for dogs treated with PGF2a or oxytocin compared to saline. Total sperm number and TNMS were greater when dogs were treated with PGF2a compared to oxytocin,
GnRH and saline (p<0.05). The subjective ease of collection score was lower for dogs receiving PGF2a compared to
GnRH or saline (p<0.05).
In summary, administration of PGF2a or oxytocin prior to semen collection increased semen volume and PGF2a increased total sperm number in the ejaculate of the
dog. It did not appear that treatment with
GnRH had an effect on semen parameters evaluated in this study.
Advisors/Committee Members: Purswell, Beverly J. (committeechair), Dascanio, John J. (committee member), Parker, Nikola A. (committee member).
Subjects/Keywords: GnRH; dog; prostaglandin F2a; oxytocin; sperm number
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APA (6th Edition):
Hess, M. B. (2002). The Effects of Prostaglandin F2a, Oxytocin and Gonadotropin Releasing Hormone on Ejaculate Characteristics in the Dog. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/31135
Chicago Manual of Style (16th Edition):
Hess, Milan B. “The Effects of Prostaglandin F2a, Oxytocin and Gonadotropin Releasing Hormone on Ejaculate Characteristics in the Dog.” 2002. Masters Thesis, Virginia Tech. Accessed March 06, 2021.
http://hdl.handle.net/10919/31135.
MLA Handbook (7th Edition):
Hess, Milan B. “The Effects of Prostaglandin F2a, Oxytocin and Gonadotropin Releasing Hormone on Ejaculate Characteristics in the Dog.” 2002. Web. 06 Mar 2021.
Vancouver:
Hess MB. The Effects of Prostaglandin F2a, Oxytocin and Gonadotropin Releasing Hormone on Ejaculate Characteristics in the Dog. [Internet] [Masters thesis]. Virginia Tech; 2002. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10919/31135.
Council of Science Editors:
Hess MB. The Effects of Prostaglandin F2a, Oxytocin and Gonadotropin Releasing Hormone on Ejaculate Characteristics in the Dog. [Masters Thesis]. Virginia Tech; 2002. Available from: http://hdl.handle.net/10919/31135
Michigan State University
28.
Villa-Godoy, Alejandro.
Influence of negative energy balance and body condition on luteal function and estrous behavior in dairy cattle.
Degree: PhD, Department of Animal Science, 1987, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:22180
Subjects/Keywords: Dairy cattle; Dairy cattle – Fertility; Estrus; Luteal phase
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APA ·
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APA (6th Edition):
Villa-Godoy, A. (1987). Influence of negative energy balance and body condition on luteal function and estrous behavior in dairy cattle. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:22180
Chicago Manual of Style (16th Edition):
Villa-Godoy, Alejandro. “Influence of negative energy balance and body condition on luteal function and estrous behavior in dairy cattle.” 1987. Doctoral Dissertation, Michigan State University. Accessed March 06, 2021.
http://etd.lib.msu.edu/islandora/object/etd:22180.
MLA Handbook (7th Edition):
Villa-Godoy, Alejandro. “Influence of negative energy balance and body condition on luteal function and estrous behavior in dairy cattle.” 1987. Web. 06 Mar 2021.
Vancouver:
Villa-Godoy A. Influence of negative energy balance and body condition on luteal function and estrous behavior in dairy cattle. [Internet] [Doctoral dissertation]. Michigan State University; 1987. [cited 2021 Mar 06].
Available from: http://etd.lib.msu.edu/islandora/object/etd:22180.
Council of Science Editors:
Villa-Godoy A. Influence of negative energy balance and body condition on luteal function and estrous behavior in dairy cattle. [Doctoral Dissertation]. Michigan State University; 1987. Available from: http://etd.lib.msu.edu/islandora/object/etd:22180
University of Missouri – Columbia
29.
Atkins, Jacqueline Anne.
Effect of ovulatory follicle size on luteal function, pregnancy rate, and late embryonic/fetal mortality in beef cattle.
Degree: 2009, University of Missouri – Columbia
URL: http://hdl.handle.net/10355/9885
► Previous reports indicate that ovulation of small dominant follicles resulted in reduced pregnancy rates compared ovulation of large follicles in cattle. A reciprocal embryo transfer…
(more)
▼ Previous reports indicate that ovulation of small dominant follicles resulted in reduced pregnancy rates compared ovulation of large follicles in cattle. A reciprocal embryo transfer approach was used to differentiate between oocyte competence and uterine environment factors that affect establishment of pregnancy following induced single ovulations of small follicles. Embryos from donor cows that ovulated a small follicle ( [less than] 12.5 mm) were transferred into recipient cows that ovulated a large follicle ([equal to] 12.5 mm) and vice versa resulting in the following treatment groups: small to large (S-L; primary effects of oocyte quality; n = 111), large to small (L-S; primary effects of uterine environment; n = 122), small to small (S-S; negative control; n = 71), and large to large (L-L; positive control; n = 50). The probability of recovering a fertilized and live embryo 7 d after breeding increased as the diameter of the ovulatory follicle increased (p = 0.01). As ovulatory follicle diameter and serum concentrations of progesterone at embryo transfer increased in the recipient cow, the probability of pregnancy increased (p = 0.05 and [less than] 0.001, respectively); however neither follicle size nor progesterone at ET in the donor cow was significant (p [greater than] 0.3). In summary, ovulatory diameter at GnRH2 was positively associated with recovery of a live embryo (possibly indicating improved oocyte competence and (or) an early uterine environment that was more conducive to embryonic/fetal development in cows that ovulated a large follicle). Pregnancy establishment following embryo transfer was related to the uterine environment established by the ovulatory follicle independent of oocyte quality.
Advisors/Committee Members: Smith, Michael Fielding (advisor).
Subjects/Keywords: Beef cattle – Pregnancy; Luteal phase; Beef cattle – Fetuses – Mortality
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APA (6th Edition):
Atkins, J. A. (2009). Effect of ovulatory follicle size on luteal function, pregnancy rate, and late embryonic/fetal mortality in beef cattle. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/9885
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Atkins, Jacqueline Anne. “Effect of ovulatory follicle size on luteal function, pregnancy rate, and late embryonic/fetal mortality in beef cattle.” 2009. Thesis, University of Missouri – Columbia. Accessed March 06, 2021.
http://hdl.handle.net/10355/9885.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Atkins, Jacqueline Anne. “Effect of ovulatory follicle size on luteal function, pregnancy rate, and late embryonic/fetal mortality in beef cattle.” 2009. Web. 06 Mar 2021.
Vancouver:
Atkins JA. Effect of ovulatory follicle size on luteal function, pregnancy rate, and late embryonic/fetal mortality in beef cattle. [Internet] [Thesis]. University of Missouri – Columbia; 2009. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/10355/9885.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Atkins JA. Effect of ovulatory follicle size on luteal function, pregnancy rate, and late embryonic/fetal mortality in beef cattle. [Thesis]. University of Missouri – Columbia; 2009. Available from: http://hdl.handle.net/10355/9885
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Melbourne
30.
McColm, Susan Clare.
The effect of exogenous oestrogens on luteal function and sexual behaviour in mares.
Degree: 1981, University of Melbourne
URL: http://hdl.handle.net/11343/113983
► The early and accurate diagnosis of pregnancy is essential for efficient equine stud management. The early identification of non-pregnant mares is important for minimizing delays…
(more)
▼ The early and accurate diagnosis of pregnancy is essential for efficient equine stud management. The early identification of non-pregnant mares is important for minimizing delays in rebreeding thereby reducing the period of intensive stud management and the inter-foaling interval. An early test for the diagnosis of pregnancy/ non-pregnancy would also provide information relating to the occurrence of abnormal corpus luteum (CL) function and early embryonic mortality, two conditions commonly seen in mares (Day 1939, Nishikawa 1959, Rossdale 1969, Allen 1972, Hughes et al 1972, Allen et al 1974, Stabenfeldt et al 1974, Palmer 1978).
The most common methods of pregnancy diagnosis are performed 40 to 45 days post-service. The methods used are rectal palpation (Lensch 1961a, 1961b, Van Niekerk 1965a, Bain 1967a, 1967b, Solomon 1971, Voss et al 1973, Allen 1974, Parker et al 1975, Voss & Pickett 1975) and measurement of pregnant mare serum gonadotrophin (PMSG) (Wide & Wide 1963, Jeffcott et al 1975). Neither method is 100% accurate in the detection of either pregnant or non-pregnant mares (Allen 1969, Allen 1974, Jeffcott et al 1969, Mitchell 1970, Solomon 1972,.Luttman & von Lepel 1973, Parker 1975). Plasma progesterone values between days 18 and 26 post-service indicate the presence of a functional CL but not necessarily a CL of pregnancy (Squires et al 1974a, 1974b, 1975, Van Niekerk et al 1975). There is then a definite requirement for an early and accurate test for the diagnosis of pregnancy and the detection of non-pregnancy.
The oestrogen treatment test (OTT) was first used for the early diagnosis of pregnancy in mares by Nishikawa in 1952. The lack of oestrous behaviour after treatment with 3 to 5 mg stilboestrol diproprionate (S) on day 17 to 20 post-service was claimed to be 95% (Reddy.& Khan 1976) to 100% (Nishikawa 1959) accurate in the diagnosis of pregnancy. But, there is evidence from other species to suggest that exogenous oestrogens can cause luteolysis and. abortion in pregnant animals and interfere with cyclic activity Of served non-pregnant animals. Since both pregnant and non-pregnant mares receive S in the OTT it was considered important to. determine the effects of S on luteal function in both pregnant and non-pregnant mares. There is insufficient evidence relating to the effects of exogenous oestrogens. in. non-pregnant mares but several reports have shown a . variety of both physiological and behavioural effects to occur in other species depending on the dose rates used and the parameters studied.
The main, aims of the research reported in this thesis were:
(i) The establishment of a radioimmunoassay for progesterone as a means of assessing luteal function
(ii) to define the effects of stilboestrol diproprionate on luteal function and sexual behaviour of pregnant and non-pregnant mares
(iii) to determine the efficacy of the OTT as a test for .pregnancy diagnosis when used alone or in conjunction with other methods currently in use
(iv) to investigate the mechanisms by which exogenous…
Subjects/Keywords: Estrogen; Fertility; Horses; Luteal phase; Mares; Physiological effect; Reproduction
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APA (6th Edition):
McColm, S. C. (1981). The effect of exogenous oestrogens on luteal function and sexual behaviour in mares. (Masters Thesis). University of Melbourne. Retrieved from http://hdl.handle.net/11343/113983
Chicago Manual of Style (16th Edition):
McColm, Susan Clare. “The effect of exogenous oestrogens on luteal function and sexual behaviour in mares.” 1981. Masters Thesis, University of Melbourne. Accessed March 06, 2021.
http://hdl.handle.net/11343/113983.
MLA Handbook (7th Edition):
McColm, Susan Clare. “The effect of exogenous oestrogens on luteal function and sexual behaviour in mares.” 1981. Web. 06 Mar 2021.
Vancouver:
McColm SC. The effect of exogenous oestrogens on luteal function and sexual behaviour in mares. [Internet] [Masters thesis]. University of Melbourne; 1981. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/11343/113983.
Council of Science Editors:
McColm SC. The effect of exogenous oestrogens on luteal function and sexual behaviour in mares. [Masters Thesis]. University of Melbourne; 1981. Available from: http://hdl.handle.net/11343/113983
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Open Access Theses and Dissertations
