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You searched for subject:(Jadomycin). Showing records 1 – 3 of 3 total matches.

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1. SREEKANTHAN, SREENIWAS. SHOCK INDUCED JADOMYCIN PRODUCTION FROM STREPTOMYCES VENEZUELAE ISP5230 VS1099.

Degree: Master of Applied Science, Department of Process Engineering and Applied Science, 2013, Dalhousie University

The production of jadomycin by Streptomyces venezuelae was carried out by applying various environmental stress conditions on the culture in nutrient deprived, amino acid rich MSM production media. The effects of culture transfer time (18, 21 and 24 h) from the growth media to the production media, heat shock at different temperatures (35, 40, 45 and 50°C) and exposure times (0.5 and 1 h), alcohol shock using ethanol and methanol at various concentrations (3.0, 4.5, 6.0 and 7.5 % v/v), multiple shocking by 3 % v/v ethanol at various times [once (0 h), twice (0, 3 h) and three times (0, 3 and 6 h)] and ethanol shock with various concentrations (3.0, 4.5 and 6.0 % v/v) and varying nitrogen concentrations (45, 60 and 75 mM) in the media on the growth and activity of S.venezuelae and jadomycin production were investigated. The growth of S.venezuelae was determined by measuring cell number and TF yield and the Jadomycin B was determined by measuring the optical density (AU¬526nm). Shocking the culture that was transferred after 18 h of growth in the growth medium produced the highest jadomycin, cell growth and activity compared to those that were transfered after 21 and 24 h. The culture shocked at a temperature of 40°C for 1 h produced the highest jadomycin. Higher temperature and/or longer exposure time resulted in poor cell growth and activity. The ethanol concentration of 4.5 % v/v produced the highest jadomycin. The culture shocked with ethanol once (0 h) produced higher jadomycin, cell growth and activity compared to the cultures shocked twice [(0, 3 h) and three times (0, 3 and 6 h)]. A nitrogen concentration of 75 mM with 4.5 % v/v in the production media increased cell growth and jadomycin production. The results showed that shocking the culture once with ethanol (3 % v/v) produced 81.5 % more jadomycin than shocking with heat at a temperature of 45°C for 1 h. However, increasing the ethanol concentration to 4.5 % v/v produced 10.8 % more jadomycin than 3 % v/v ethanol. By increasing the nitrogen concentration to 75 mM in the production medium and shocking with the 4.5 % v/v ethanol further increased the jadomycin production by 13.9 % above that without nitrogen addition. Therefore, the treatment with ethanol (4.5 % v/v) and nitrogen (75 mM) is recommended for jadomycin production. Advisors/Committee Members: N/A (external-examiner), DR. MARK GIBSON (graduate-coordinator), DR. SUZANNE M. BUDGE (thesis-reader), DR. ABDEL GHALY, DR. SU-LING BROOKS (thesis-supervisor), Not Applicable (ethics-approval), No (manuscripts), No (copyright-release).

Subjects/Keywords: JADOMYCIN

…Table 6.1. Changes in growth, activity and jadomycin production of S.venezuelae during… …164 Table 6.2. Jadomycin kinetic parameters a affected by culture transfer time… …169 Table 6.3. Changes in growth, activity and jadomycin production of S.venezuelae as… …171 Table 6.4. Jadomycin kinetic parameters as affected by heat shock temperature… …178 Table 6.5. Changes in growth, activity and jadomycin production of S.venezuelae as… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

SREEKANTHAN, S. (2013). SHOCK INDUCED JADOMYCIN PRODUCTION FROM STREPTOMYCES VENEZUELAE ISP5230 VS1099. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/40650

Chicago Manual of Style (16th Edition):

SREEKANTHAN, SREENIWAS. “SHOCK INDUCED JADOMYCIN PRODUCTION FROM STREPTOMYCES VENEZUELAE ISP5230 VS1099.” 2013. Masters Thesis, Dalhousie University. Accessed May 29, 2020. http://hdl.handle.net/10222/40650.

MLA Handbook (7th Edition):

SREEKANTHAN, SREENIWAS. “SHOCK INDUCED JADOMYCIN PRODUCTION FROM STREPTOMYCES VENEZUELAE ISP5230 VS1099.” 2013. Web. 29 May 2020.

Vancouver:

SREEKANTHAN S. SHOCK INDUCED JADOMYCIN PRODUCTION FROM STREPTOMYCES VENEZUELAE ISP5230 VS1099. [Internet] [Masters thesis]. Dalhousie University; 2013. [cited 2020 May 29]. Available from: http://hdl.handle.net/10222/40650.

Council of Science Editors:

SREEKANTHAN S. SHOCK INDUCED JADOMYCIN PRODUCTION FROM STREPTOMYCES VENEZUELAE ISP5230 VS1099. [Masters Thesis]. Dalhousie University; 2013. Available from: http://hdl.handle.net/10222/40650


Dalhousie University

2. Martinez-Farina, Camilo. Investigations into the Biosynthesis, Derivatization, and Purification of Jadomycins.

Degree: MS, Department of Chemistry, 2015, Dalhousie University

Nature produces many clinically used medicines in the form of natural products. These compounds can be isolated from a variety of sources, but bacteria have been shown to be the most prolific source. The strain Streptomyces has been thoroughly investigated for such natural products, with Streptomyces venezuelae ISP5230 showing the ability to produce the clinically used antibiotic chloramphenicol, as well as the jadomycins, a family of secondary metabolites. These secondary metabolites are produced through a biosynthetic pathway where the incorporation of the amino acid into the jadomycin structure is likely non-enzymatic. This allows for jadomycins to be readily derivatized, where upwards of twenty-five derivatives have been previously isolated. This work presents the amplification of the jadomycin library through the production of novel jadomycins, as well as their further diversification through the use of synthetic derivatization. The study of jadomycins is not only important chemically, but also biologically because they have been shown to possess anti-cancerous properties. For this reason, the two synthetic derivatives were assessed for biological activities and their results are discussed herein. Investigations were also carried out to develop a method to assess biological activity by nuclear magnetic resonance, and are presented. Finally, investigations into the jadomycin purification methodology were carried out using JadX, a potential regulatory protein in the jadomycin biosynthesis, and are discussed. Advisors/Committee Members: n/a (external-examiner), Dr. Mark Stradiotto (graduate-coordinator), Dr. T. Bruce Grindley (thesis-reader), Dr. Frances L. Cozens (thesis-reader), Dr. David L. Jakeman (thesis-supervisor), Not Applicable (ethics-approval), Yes (manuscripts), Yes (copyright-release).

Subjects/Keywords: Natural Products; Jadomycin; Precursor-Directed Biosynthesis; WaterLOGSY

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Martinez-Farina, C. (2015). Investigations into the Biosynthesis, Derivatization, and Purification of Jadomycins. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/56323

Chicago Manual of Style (16th Edition):

Martinez-Farina, Camilo. “Investigations into the Biosynthesis, Derivatization, and Purification of Jadomycins.” 2015. Masters Thesis, Dalhousie University. Accessed May 29, 2020. http://hdl.handle.net/10222/56323.

MLA Handbook (7th Edition):

Martinez-Farina, Camilo. “Investigations into the Biosynthesis, Derivatization, and Purification of Jadomycins.” 2015. Web. 29 May 2020.

Vancouver:

Martinez-Farina C. Investigations into the Biosynthesis, Derivatization, and Purification of Jadomycins. [Internet] [Masters thesis]. Dalhousie University; 2015. [cited 2020 May 29]. Available from: http://hdl.handle.net/10222/56323.

Council of Science Editors:

Martinez-Farina C. Investigations into the Biosynthesis, Derivatization, and Purification of Jadomycins. [Masters Thesis]. Dalhousie University; 2015. Available from: http://hdl.handle.net/10222/56323


Dalhousie University

3. Dupuis, Stephanie. Precursor-Directed Biosynthesis of Novel Jadomycins and Expansion of the Jadomycin Library.

Degree: MS, Department of Chemistry, 2010, Dalhousie University

Jadomycins are secondary metabolites produced by Streptomyces venezuelae ISP5230 VS1099 in response to conditions of stress such as heat or ethanol shock. They have been shown to exhibit antibiotic and anticancer activity. Unique structural features of the jadomycins include a rare 2,6-dideoxysugar, L-digitoxose, and an oxazolone ring with an amino acid component. Previous studies have revealed that jadomycin derivatives can be produced by altering the amino acid in S. venezuelae ISP5230 VS1099 culture media which becomes incorporated into the oxazolone ring. One jadomycin from a proteogenic amino acid and three new jadomycins from non-proteogenic amino acids have been successfully produced on a large scale (4 mg/L to 12 mg/L, 2 L) and characterized using mass spectrometry, infrared spectroscopy, UV-visible spectroscopy, and nuclear magnetic resonance spectroscopy. One of these contains a terminal alkyne functionality and has been used in cycloaddition reactions with various azides to produce a library of triazole-containing jadomycins. Advisors/Committee Members: Dr. Frances Cozens (external-examiner), Dr. Mark Stradiotto (graduate-coordinator), Dr. Jean Burnell (thesis-reader), Dr. Ray Syvitski (thesis-reader), Dr. David Jakeman (thesis-supervisor), Not Applicable (ethics-approval), Yes (manuscripts), Not Applicable (copyright-release).

Subjects/Keywords: jadomycin; secondary metabolite; precursor-directed biosynthesis; cycloaddition; natural product

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Dupuis, S. (2010). Precursor-Directed Biosynthesis of Novel Jadomycins and Expansion of the Jadomycin Library. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/13053

Chicago Manual of Style (16th Edition):

Dupuis, Stephanie. “Precursor-Directed Biosynthesis of Novel Jadomycins and Expansion of the Jadomycin Library.” 2010. Masters Thesis, Dalhousie University. Accessed May 29, 2020. http://hdl.handle.net/10222/13053.

MLA Handbook (7th Edition):

Dupuis, Stephanie. “Precursor-Directed Biosynthesis of Novel Jadomycins and Expansion of the Jadomycin Library.” 2010. Web. 29 May 2020.

Vancouver:

Dupuis S. Precursor-Directed Biosynthesis of Novel Jadomycins and Expansion of the Jadomycin Library. [Internet] [Masters thesis]. Dalhousie University; 2010. [cited 2020 May 29]. Available from: http://hdl.handle.net/10222/13053.

Council of Science Editors:

Dupuis S. Precursor-Directed Biosynthesis of Novel Jadomycins and Expansion of the Jadomycin Library. [Masters Thesis]. Dalhousie University; 2010. Available from: http://hdl.handle.net/10222/13053

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