subject:(In vivo)

1. Nader, Joëlle. Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy.

Degree: Docteur es, Biologie, médecine et santé, 2017, Nantes

URL: http://www.theses.fr/2017NANT1043

►

Au cours de ma thèse, j’ai travaillé sur deux sujets complémentaires. Le premier a concerné l’étude des interactions entre les cellules tumorales et leur microenvironnement,… (more)

▼

Au cours de ma thèse, j’ai travaillé sur deux sujets complémentaires. Le premier a concerné l’étude des interactions entre les cellules tumorales et leur microenvironnement, dans quatre modèles de mésothéliomes malins (MM) de rat. Les analyses histologiques du stroma, associées à des analyses protéomiques et de l’expression de différentes cytokines/chimiokines ont permis d’identifier trois stades d’invasivité croissante, associés à des modifications quantitatives de nombreuses protéines et à un infiltrat immunitaire décroissant. La tumeur la plus invasive a été caractérisée par une immunosuppression avec un profil moléculaire spécifique augmentant le potentiel métastatique. Le second sujet a concerné l’évaluation de l’efficacité de la virothérapie anti-tumorale, basée sur l’utilisation de la souche vaccinale du virus de la rougeole MV et de son variant MV-ΔC, pour le traitement du MM. Les deux virus ont induit des régressions tumorales chez des souris NOD SCID transplantées avec deux lignées de MM humains, le MV-ΔC amplifiant cet effet en induisant une mort cellulaire plus rapide, attestée par une réduction plus marquée de la masse tumorale. Ce potentiel apoptotique est associé, in vitro, à une production accrue du signal de danger HMGB1 et à la synthèse d’une grande quantité d’ARN double brin viral. Ces cellules infectées par le MV-ΔC sont aussi capables de favoriser la maturation des cellules dendritiques grâce à la réplication virale et à l’activation de Protéine Kinase R. Ce travail de caractérisation de nouveaux modèles immunocompétents et de nouvelles stratégies thérapeutiques permet d'envisager un meilleur traitement des patients souffrant de mésothéliome.

As a PhD student, I worked in parallel on two complementary subjects. The first one concerned the study of the interactions between tumor cells and their microenvironment in four models of rat malignant mesothelioma (MM), differing in both their immune infiltrate and their metastatic potential. Histological analyses of stroma, together with proteomic analyses and expression of different cytokines, chemokines and growth factors, led us to the identification of three stages of increasing invasiveness, associated with quantitative changes in many proteins and a decreased immune infiltrate. The most invasive tumor was characterized by immunosuppression with a specific molecular profile increasing the metastatic potential. The second topic was the evaluation of the efficacy of anti-tumor virotherapy, based on the use of the Schwarz strain of measles virus (MV) and its variant MV-ΔC for the treatment of MM. Both viruses induced tumor regressions in NOD SCID mice transplanted with two human MM cell lines, but MV-ΔC amplified this effect by inducing faster cell death, as revealed by a marked reduction of the tumor mass. This apoptotic potential is associated, in vitro, with an increased production of the danger signal HMGB1 and the synthesis of a large amount of viral double-stranded RNA. These MV-ΔC-infected cells are also capable of promoting the maturation of…

Advisors/Committee Members: Pouliquen, Daniel (thesis director), Boisgerault, Nicolas (thesis director).

Subjects/Keywords: Modèles in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nader, J. (2017). Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy. (Doctoral Dissertation). Nantes. Retrieved from http://www.theses.fr/2017NANT1043

Chicago Manual of Style (16^th Edition):

Nader, Joëlle. “Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy.” 2017. Doctoral Dissertation, Nantes. Accessed March 07, 2021. http://www.theses.fr/2017NANT1043.

MLA Handbook (7^th Edition):

Nader, Joëlle. “Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy.” 2017. Web. 07 Mar 2021.

Vancouver:

Nader J. Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy. [Internet] [Doctoral dissertation]. Nantes; 2017. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2017NANT1043.

Council of Science Editors:

Nader J. Modèles précliniques d’étude du mésothéliome : application à l’évaluation de la virothérapie anti-tumorale in vivo : Experimental models of malignant mesothelioma for the evaluation of in vivo anti-tumoral virotherapy. [Doctoral Dissertation]. Nantes; 2017. Available from: http://www.theses.fr/2017NANT1043

2. Halilaj, Eni. Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis.

Degree: PhD, Biomedical Engineering, 2015, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:419393/

► Humans have a unique opposable thumb that maintains a fine balance between mobility and stability to allow motions that span from precision handling to power… (more)

▼ Humans have a unique opposable thumb that maintains a fine balance between mobility and stability to allow motions that span from precision handling to power grasping. Much of the versatility of the thumb is due to the saddle geometry of first carpometacarpal (CMC) joint. Located at the base of the thumb, the CMC joint is the most common site of reconstructive surgery in the upper extremity. Nearly 40% of postmenopausal women are affected by CMC osteoarthritis (OA)—a progressive degenerative disease of articular cartilage, bone, and synovium that results in decreased dexterity and partial impairment of the upper extremity. Although current treatment options can alleviate pain, they cannot restore the lost strength and mobility, or halt disease progression, because the pathophysiology of the disease is poorly understood. While both biological and mechanical factors are implicated in the etiology of CMC OA, the synergistic mechanism of disease evolution remains unclear, partially due to a paucity of published data on CMC joint biomechanics in healthy and diseased populations. Accordingly, the objective of this work was to study CMC joint biomechanics using in vivo image-based techniques, and to determine if there are any differences with sex, age, or OA onset that could point to the etiology of the disease. 155 subjects—healthy men and women of two age groups and patients with early stage CMC OA—were recruited for the study. Sequential computed tomography scans of the hands and wrists of all the participants were acquired while they performed functional tasks and range-of-motion activities. The imaging data were used to study joint motion, stability, contact mechanics, articular shape and congruence, as well as to model soft tissue: cartilage and ligaments. The findings presented here suggest that there may not be a mechanical predisposition to CMC OA, but that women may be more susceptible to cartilage loss due to systemic changes with aging. In addition to channeling future research in the right direction, the furthered understanding of CMC joint biomechanics should serve to refine the current surgical procedures and to inspire new conservative options for disease treatment and prevention. Advisors/Committee Members: Crisco, Joseph (Director), Laidlaw, David (Director), Sharon, Swartz (Reader), Braden, Flemming (Reader), Amy, Ladd (Reader).

Subjects/Keywords: in vivo joint kinematics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Halilaj, E. (2015). Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:419393/

Chicago Manual of Style (16^th Edition):

Halilaj, Eni. “Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis.” 2015. Doctoral Dissertation, Brown University. Accessed March 07, 2021. https://repository.library.brown.edu/studio/item/bdr:419393/.

MLA Handbook (7^th Edition):

Halilaj, Eni. “Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis.” 2015. Web. 07 Mar 2021.

Vancouver:

Halilaj E. Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis. [Internet] [Doctoral dissertation]. Brown University; 2015. [cited 2021 Mar 07]. Available from: https://repository.library.brown.edu/studio/item/bdr:419393/.

Council of Science Editors:

Halilaj E. Image-Based Analysis of Thumb Carpometacarpal Joint Biomechanics in Health and Early Osteoarthritis. [Doctoral Dissertation]. Brown University; 2015. Available from: https://repository.library.brown.edu/studio/item/bdr:419393/

University of Arizona

3. Nguyen, Christopher David. Light Sheet Reflectance Microscopy .

Degree: 2019, University of Arizona

URL: http://hdl.handle.net/10150/633125

► Many human diseases are diagnosed by the histopathological analysis of suspicious lesions where cellular morphological changes are examined at microscopic resolution. Reflectance confocal microscopy (RCM)… (more)

Subjects/Keywords: in vivo; light sheet; microscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, C. D. (2019). Light Sheet Reflectance Microscopy . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/633125

Chicago Manual of Style (16^th Edition):

Nguyen, Christopher David. “Light Sheet Reflectance Microscopy .” 2019. Masters Thesis, University of Arizona. Accessed March 07, 2021. http://hdl.handle.net/10150/633125.

MLA Handbook (7^th Edition):

Nguyen, Christopher David. “Light Sheet Reflectance Microscopy .” 2019. Web. 07 Mar 2021.

Vancouver:

Nguyen CD. Light Sheet Reflectance Microscopy . [Internet] [Masters thesis]. University of Arizona; 2019. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10150/633125.

Council of Science Editors:

Nguyen CD. Light Sheet Reflectance Microscopy . [Masters Thesis]. University of Arizona; 2019. Available from: http://hdl.handle.net/10150/633125

4. Risticevic, Sanja. Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/7006

► The objective of the current research project relies on implementation of solvent-free, green and environmentally friendly solid phase microextraction (SPME) sample preparation alternative in the… (more)

▼ The objective of the current research project relies on implementation of solvent-free, green and environmentally friendly solid phase microextraction (SPME) sample preparation alternative in the area of complex sample characterization. The advantages that the technique offers in comparison to traditional methods of sample preparation including solvent-free implementation, short sample preparation times, small sample amount requirements, advanced automation capability and minimization of matrix effects are effectively employed during ex vivo and laboratory investigations of complex samples. More important, the underlying features of the technique including miniaturized format, nonexhaustive extraction recoveries and on-site compatibility were fully exploited in order to investigate the metabolome of biological systems directly on the site. Hence, in vivo SPME extraction format was employed in direct immersion SPME sampling of biological systems, hence eliminating the crucial prerequisites associated with multiple preparative steps and incorporation of metabolism quenching that are encountered during implementation of traditional sample preparation methods in global metabolite analysis. Furthermore, in vivo sampling format was hyphenated to comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (GCxGC-ToFMS) for high-resolution sampling of volatile and semivolatile metabolites in ‘Honeycrisp’ apples. The initial stages of the project involved evaluation of performance characteristics of commercial SPME extraction coatings in terms of extraction selectivity, extraction sensitivity and desorption efficiency by employing headspace SPME analysis of both aqueous standards spiked with representative volatile and semivolatile metabolites as well as the apple homogenate. DVB/CAR/PDMS coating was selected on the basis of optimum metabolite coverage and extraction sensitivity and was consequently employed during ex vivo and in vivo sampling assays corresponding to determination of volatile and semivolatile metabolites. The former extraction methodology incorporated appropriate sample preparation steps for quenching metabolic activity so that the relevant metabolome profile is not biased against unstable metabolites and those that are susceptible to inter-metabolite conversions which adversely impact preservation of metabolite identity. The two sample preparation assays were compared in terms of metabolite coverage and analytical precision in order to identify SPME route toward characterization of more representative metabolome and determination of instantaneous and more ‘true’ metabolism snapshoot. This is the first report illustrating the implementation of in vivo direct immersion SPME assay for non invasive determination of endogenous fruit metabolites whose profiles and contents are highly correlated to a multitude of influential fruit quality traits.

Subjects/Keywords: solid phase microextraction; in vivo

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Risticevic, S. (2012). Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7006

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Risticevic, Sanja. “Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome.” 2012. Thesis, University of Waterloo. Accessed March 07, 2021. http://hdl.handle.net/10012/7006.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Risticevic, Sanja. “Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome.” 2012. Web. 07 Mar 2021.

Vancouver:

Risticevic S. Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10012/7006.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Risticevic S. Solid phase microextraction coupled to comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for metabolite profiling of apples: Potential of non-invasive in vivo sampling assay in characterization of metabolome. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/7006

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

5. Morris, Bart Alan. An Efficient Approach to EPID Transit Dosimetry.

Degree: MS, Physical Sciences and Mathematics, 2014, Louisiana State University

URL: etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219

► Introduction: In order to maintain uniform standards in the accuracy of fractionated radiation therapy, quantification of the delivered dose per fraction accuracy is required. The… (more)

▼ Introduction: In order to maintain uniform standards in the accuracy of fractionated radiation therapy, quantification of the delivered dose per fraction accuracy is required. The pupose of this study was to investigate the feasibility of a transit dosimetry method using the electronic portal imaging device (EPID) for dose delivery error detection and prevention. Methods and Materials: In the proposed method, 2D predicted transit images were generated for comparison with online images acquired during treatment. Predicted transit images were generated by convolving through-air EPID measurements of each field with pixel-specific kernels selected from a library of pre-calculated Monte Carlo pencil kernels of various radiological thicknesses. The kernel used for each pixel was selected based on the calculated radiological thickness of the patient along the line joining the pixel to the virtual source. The accuracy of the technique was evaluated in homogeneous plastic water phantoms, a heterogeneous cylindrical phantom, and an anthropomorphic head phantom. Gamma analysis was used to quantify the accuracy of the technique for the various cases. Results: In the comparison between the measured and predicted images, an average of 99.4% of the points in passed a 3%/ 3 mm gamma for the homogeneous plastic water phantoms. Points for the heterogeneous cylindrical phantom analysis had a 94.6% passing rate. For the anthropomorphic head phantom, an average of 98.3% and 96.6% of points passed the 5%/3mm and 3%/3mm gamma criteria, respectively for all field sizes. Failures occurred typically at points when object thickness was changing rapidly or at boundaries between materials, and at the edges of large fields. Discussion: The results suggested that the proposed transit dosimetry method is a feasible approach to in vivo dose monitoring. The gamma analysis passing rates are within the accuracy needed for transit dosimetry. Future research efforts should include evaluation of the method for more complex treatment techniques and assessment of the sensitivity to changes in EPID or linac hardware, as well as characterization of any dependency the method may have on image ghosting or lag, gantry angle, or long-term stability.

Subjects/Keywords: EPID dosimetry transit in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morris, B. A. (2014). An Efficient Approach to EPID Transit Dosimetry. (Masters Thesis). Louisiana State University. Retrieved from etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219

Chicago Manual of Style (16^th Edition):

Morris, Bart Alan. “An Efficient Approach to EPID Transit Dosimetry.” 2014. Masters Thesis, Louisiana State University. Accessed March 07, 2021. etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219.

MLA Handbook (7^th Edition):

Morris, Bart Alan. “An Efficient Approach to EPID Transit Dosimetry.” 2014. Web. 07 Mar 2021.

Vancouver:

Morris BA. An Efficient Approach to EPID Transit Dosimetry. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Mar 07]. Available from: etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219.

Council of Science Editors:

Morris BA. An Efficient Approach to EPID Transit Dosimetry. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-06192014-080930 ; https://digitalcommons.lsu.edu/gradschool_theses/3219

McGill University

6. Athlan, Eric S. Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins.

Degree: PhD, Department of Biochemistry, 1998, McGill University

URL: https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732

►

L'expression des trois protéines de neurofilaments (NF) NFL, NFM et NFH ainsi que I'a-intemexine et la périphérine a été déterminée par immunobuvardage "Western" durant Ie… (more)

▼

L'expression des trois protéines de neurofilaments (NF) NFL, NFM et NFH ainsi que I'a-intemexine et la périphérine a été déterminée par immunobuvardage "Western" durant Ie développement de neurones embryonnaires de ganglions dorsaux en culture. Au jour 0 de la culture, les neurones contenaient surtout de I'a-intemexine. La quantité de périphérine a augmenté de façon significative après 1-2 jours en culture, celle de NFL et NFM après 2-3 jours, et celle de NFH après 5-6 jours. L'analyse des cultures par immunofluorescence indirecte a révélé que les cinq protéines de filament intermédiaire neuronaux (Fin) étaient co-Iocalisées dans tout les compartiments des neurones. L'analyse d'extraits cellulaires solubles au Triton X-100 suite à un traitement a I'acide okadaïque a indiqué que la solubilisation de I'a-intemexine et de la périphérine suivaient Ie même profile que pour NFL, NFM et NFH. L'incubation de ces extraits cellulaires avec des anticorps spécifiques pour chaque protéine permit la co-immunoprécipitation de NFH avec NFL, NFM avec NFL, NFM avec a-internexine et a-internexine avec peripherine démontrant ainsi que ces cinq protéines sont associées dans Ie réseau de Fin des neurones sensorielles en culture.

The developmental profile of the neurofilament (NF) triplet proteins, a-internexin and peripherin in cultured embryonal dorsal root ganglion neurons was determined by Western blot analysis. At day 0 in culture, the neurons contained mostly a-internexin. A significant increase in peripherin levels was seen at days 1-2, in the mid-sized (NFM) and low molecular weight (NFL) NF subunits at days 2-3, and in the high molecular weight (NFH) NF subunit at days 5-6. Immunofluorescence microscopy showed that the five neuronal intermediate filament (nlF) proteins were colocalized in all neuronal cell bodies and neurites. Analysis of Triton X-100-soluble extracts from okadaic acid-treated DRG cultures revealed that peripherin and a-internexin followed the same fragmentation pattern observed for NFs. When these extracts were incubated with antibodies specific for the individual proteins, coimmunoprecipitation of NFH with NFL, NFM with NFL, NFM with a-internexin, and a-internexin with peripherin were observed, demonstrating that nlF proteins in cultured sensory neurons form a highly integrated network.

Advisors/Committee Members: Mushynski, Walter.

Subjects/Keywords: In Vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Athlan, E. S. (1998). Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732

Chicago Manual of Style (16^th Edition):

Athlan, Eric S. “Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins.” 1998. Doctoral Dissertation, McGill University. Accessed March 07, 2021. https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732.

MLA Handbook (7^th Edition):

Athlan, Eric S. “Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins.” 1998. Web. 07 Mar 2021.

Vancouver:

Athlan ES. Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins. [Internet] [Doctoral dissertation]. McGill University; 1998. [cited 2021 Mar 07]. Available from: https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732.

Council of Science Editors:

Athlan ES. Study of In Vivo and In Vitro Associations Between Neuronal Intermediate Filament Proteins. [Doctoral Dissertation]. McGill University; 1998. Available from: https://escholarship.mcgill.ca/downloads/c821gn513.pdf ; https://escholarship.mcgill.ca/concern/theses/sf2687732

University of Georgia

7. Ross, Teresa Adrianna Coleman. In vivo analysis of wild-type and variant human ferrochelatases.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/24481

► Ferrochelatase is the terminal enzyme in the heme biosynthetic pathway and catalyzes the insertion of ferrous iron into protoporphyrin IX in the formation of protoheme… (more)

Subjects/Keywords: Ferrochelatase; S. cerevisiae; in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ross, T. A. C. (2014). In vivo analysis of wild-type and variant human ferrochelatases. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/24481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ross, Teresa Adrianna Coleman. “In vivo analysis of wild-type and variant human ferrochelatases.” 2014. Thesis, University of Georgia. Accessed March 07, 2021. http://hdl.handle.net/10724/24481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ross, Teresa Adrianna Coleman. “In vivo analysis of wild-type and variant human ferrochelatases.” 2014. Web. 07 Mar 2021.

Vancouver:

Ross TAC. In vivo analysis of wild-type and variant human ferrochelatases. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10724/24481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ross TAC. In vivo analysis of wild-type and variant human ferrochelatases. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/24481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

8. Gupta, Bakul. Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo.

Degree: Chemistry, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true

► Porous silicon (PSi) photonic crystals have aroused interest as chemical and biological sensing devices and have been used as label-free optical biosensors in recent years.… (more)

▼ Porous silicon (PSi) photonic crystals have aroused interest as chemical and biological sensing devices and have been used as label-free optical biosensors in recent years. The advantage of using PSi as a sensor comes from the ease of fabrication and high quality optics. The recent advances in modifying the surface of these porous materials with different surface chemistries have been utilized to add stability to the otherwise unstable freshly etched PSi surface. The results presented in this thesis have been obtained while developing porous silicon microsensors for their use in detecting protease enzyme activity in vivo. The research presented here primarily focuses on the additions made to the surface modification strategies to add functionalities to the surface for specific target (enzyme) detection and quantification both in vitro and in vivo. A specific class of photonic crystals, rugate filters were fabricated on p-type silicon and used as transducers to transform biological changes to easily read-out optical signals. The presence of a high reflectivity resonant stop-band in the reflectance spectra of these filters made them an ideal choice to be used as a transducing element thereby adding sensitivity to the biosensor. A chemical route based on hydrosilylation of alkynes was adopted to modify the freshly etched PSi surface. This was followed by the copper (I) - catalysed azide cycloaddition reaction using a synthetic anti-fouling polymer to resist non-specific adsorption of biomolecules. Immobilisation of enzyme responsive peptides to these organic layers added specificity to the PSi structure. This specificity was demonstrated by the selective detection of matrix metalloproteases (MMP), MMP-2 and MMP-9 enzymes released from different primary ocular cells by measuring optical blue shifts of these PSi rugate filters. Detection of pg quantities of these MMPs was achieved using these microsensors. The sensitivity achieved by the PSi microsensors was lower than that achieved with zymography; the standard protease detection technique. This functionalisation of the surface with peptides adds versatility to the surface and allows for a range of other biological species to be detected for applications in biology and medicine. Towards protease detection in vivo, PSi microparticles were injected into eyes of live rabbit animal models. The microparticles were shown to be biocompatible for up to 4 weeks. The animal models were injected with a bacterial endotoxin, lipopolysaccharide to cause endotoxin induced uveitis (EIU) with associated up regulation of the release of proteases inside the eye. Efforts were made to detect the release of MMP-9 in vivo by the injected PSi microparticles by measuring optical changes in the reflectivity spectra of these particles in real time. Digestion of the peptide was detected down to 1.3 picomoles (11.7 nM) of the protease in the uveitis infected eye. The detection of proteases both in vitro and in vivo via the use of robust, selective and flexible surface chemistry on PSi photonic… Advisors/Committee Members: Gooding, Prof. J. Justin, Chemistry, Faculty of Science, UNSW, Reece, Dr. Peter J., Physics, Faculty of Science, UNSW.

Subjects/Keywords: In vivo; Porous silicon; Protease

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gupta, B. (2013). Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Gupta, Bakul. “Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo.” 2013. Doctoral Dissertation, University of New South Wales. Accessed March 07, 2021. http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Gupta, Bakul. “Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo.” 2013. Web. 07 Mar 2021.

Vancouver:

Gupta B. Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Mar 07]. Available from: http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true.

Council of Science Editors:

Gupta B. Bioresponsive porous silicon photonic crystals for monitoring protease activity in vivo. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53296 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11991/SOURCE02?view=true

University of Manchester

9. Harrison, James Andrew Roy. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.

Degree: 2018, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017

► Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no… (more)

▼ Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no large scale analysis has been performed for hepatic uptake data. The primary aim of this thesis was therefore to investigate the utility of various clearance parameters generated in hepatocellular assays for the prediction of in vivo clearance. Large scale literature analyses were performed for uptake data in both rat and human hepatocytes. In the rat, it was highlighted that over-prediction was the predominant issue for suspension and media loss hepatocyte assays. Conversely, monolayer and SCH assays suffered from under-prediction. However, in human hepatocytes under-prediction was observed in all assay formats. Use of empirical scaling factors improved predictions in both species, and are recommended for future use. The media loss assay, a method described by Soars et al[1], was further developed in rat hepatocytes through inclusion of transporter and metabolic enzyme inhibitors. Using a two-compartment model, individual clearance parameters (CLmet, CLactive and CLpassive) were estimated, and were also used to estimate binding and partitioning terms (Kp, Kpu and fucell). IVIVE of data produced from this assay resulted in a lower bias than had been noted from literature data. However, it was hypothesised that additional clearance parameters could be used in a mechanistic approach to further improve predictions. SCH assays were performed to generate estimates of uptake rates, as well as efflux rates from both the sinusoidal and canalicular membranes. Combining clearance terms from both the media loss and SCH assays using the CLint,total term led to less bias when predicting in vivo clearance than observed using uptake or metabolism data alone. Additionally, the use of empirical scaling factors identified from the literature analysis led to further reduction in prediction bias. Future work must now focus on the application of this research to human hepatocytes. It is concluded that the work presented in this thesis provides evidence for the usefulness of both uptake and extended clearance terms, in conjunction with empirical scaling methods, for the prediction of in vivo clearance. Adaptation of the media loss assay allowed the estimation of several key pharmacokinetic parameters. Although some of these are not always useful in a quantitative fashion, they remain essential properties of a compound that must be considered when predicting behaviour within the body. Advisors/Committee Members: Houston, James.

Subjects/Keywords: Hepatocytes; Pharmacokinetics; In vivo prediction

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harrison, J. A. R. (2018). Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017

Chicago Manual of Style (16^th Edition):

Harrison, James Andrew Roy. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Doctoral Dissertation, University of Manchester. Accessed March 07, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017.

MLA Handbook (7^th Edition):

Harrison, James Andrew Roy. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Web. 07 Mar 2021.

Vancouver:

Harrison JAR. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Mar 07]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017.

Council of Science Editors:

Harrison JAR. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Doctoral Dissertation]. University of Manchester; 2018. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313017

University of Manchester

10. Harrison, James. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.

Degree: PhD, 2018, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108

► Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no… (more)

▼ Predictive pharmacokinetics now forms a critical part of the drug discovery process. However, metabolic data has been demonstrated to under-predict in vivo clearance, while no large scale analysis has been performed for hepatic uptake data. The primary aim of this thesis was therefore to investigate the utility of various clearance parameters generated in hepatocellular assays for the prediction of in vivo clearance. Large scale literature analyses were performed for uptake data in both rat and human hepatocytes. In the rat, it was highlighted that over-prediction was the predominant issue for suspension and media loss hepatocyte assays. Conversely, monolayer and SCH assays suffered from under-prediction. However, in human hepatocytes under-prediction was observed in all assay formats. Use of empirical scaling factors improved predictions in both species, and are recommended for future use. The media loss assay, a method described by Soars et al[1], was further developed in rat hepatocytes through inclusion of transporter and metabolic enzyme inhibitors. Using a two-compartment model, individual clearance parameters (CLmet, CLactive and CLpassive) were estimated, and were also used to estimate binding and partitioning terms (Kp, Kpu and fucell). IVIVE of data produced from this assay resulted in a lower bias than had been noted from literature data. However, it was hypothesised that additional clearance parameters could be used in a mechanistic approach to further improve predictions. SCH assays were performed to generate estimates of uptake rates, as well as efflux rates from both the sinusoidal and canalicular membranes. Combining clearance terms from both the media loss and SCH assays using the CLint,total term led to less bias when predicting in vivo clearance than observed using uptake or metabolism data alone. Additionally, the use of empirical scaling factors identified from the literature analysis led to further reduction in prediction bias. Future work must now focus on the application of this research to human hepatocytes. It is concluded that the work presented in this thesis provides evidence for the usefulness of both uptake and extended clearance terms, in conjunction with empirical scaling methods, for the prediction of in vivo clearance. Adaptation of the media loss assay allowed the estimation of several key pharmacokinetic parameters. Although some of these are not always useful in a quantitative fashion, they remain essential properties of a compound that must be considered when predicting behaviour within the body.

Subjects/Keywords: Hepatocytes; Pharmacokinetics; In vivo prediction

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harrison, J. (2018). Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108

Chicago Manual of Style (16^th Edition):

Harrison, James. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Doctoral Dissertation, University of Manchester. Accessed March 07, 2021. https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108.

MLA Handbook (7^th Edition):

Harrison, James. “Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance.” 2018. Web. 07 Mar 2021.

Vancouver:

Harrison J. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Mar 07]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108.

Council of Science Editors:

Harrison J. Utility of hepatocellular systems to measure drug transport and metabolism for prediction of in vivo drug clearance. [Doctoral Dissertation]. University of Manchester; 2018. Available from: https://www.research.manchester.ac.uk/portal/en/theses/utility-of-hepatocellular-systems-to-measure-drug-transport-and-metabolism-for-prediction-of-in-vivo-drug-clearance(2da57336-1850-47a4-82ab-24e59798283e).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.823108

11. Vautheny, Audrey. Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy.

Degree: Docteur es, Sciences de la vie et de la santé, 2019, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2019SACLS170

►

Les processus de neuro-inflammation jouent un rôle majeur dans la maladie d'Alzheimer (MA). Des études génétiques récentes démontrent cette association entre neuro-inflammation et MA et… (more)

▼

Les processus de neuro-inflammation jouent un rôle majeur dans la maladie d'Alzheimer (MA). Des études génétiques récentes démontrent cette association entre neuro-inflammation et MA et impliquent notamment un gène, TREM2, qui code pour un récepteur exprimé à la surface de la microglie. La tauopathie est une lésion caractéristique de la MA. Elle se traduit par l’hyperphosphorylation et l’agrégation intraneuronale de la protéine Tau. Les travaux sur le rôle de TREM2 dans le développement de la pathologie Tau sont peu nombreux et donnent des résultats contradictoiresAinsi, l’objectif de ma thèse est d’étudier le rôle de la neuroinflammation et de TREM2 dans la progression de la tauopathie, dans deux modèles différents. Le premier est obtenu par injection stéréotaxique de vecteurs AAV dans la couche CA1 de l’hippocampe de souris déficientes ou non en TREM2. Ces vecteurs entrainent la surexpression de différentes formes de la protéine Tau humaine et permettent de récapituler les différents stades de la tauopathie.Nous avons en parallèle utilisé un modèle transgénique plus progressif de tauopathie, la souris THY-Tau22, afin d’étudier l’influence du stade de la pathologie dans l’effet provoqué par une déficience en TREM2 sur l’évolution de la pathologie. Notre étude a mis en évidence la toxicité des formes solubles de Tau dans le modèle AAV par rapport à ses formes agrégées. Le modèle transgénique THY-Tau22 nous a permis de mettre en évidence une augmentation des lésions tauopathiques dans les souris déficientes en TREM2 par rapport aux souris qui ne le sont pas, uniquement à un stade avancé. Cela suggère que, à l’instar des modèles amyloïdes, l’effet de la déficience en TREM2 sur le décours de la tauopathie est différent en fonction du stade considéré.

Neuroinflammation processes appear to play a major role in Alzheimer's disease (AD). Recent genetic studies support this correlation between neuroinflammation and AD and include a gene, TREM2, expressed on microglial surface. Tauopathy is a characteristic lesion of AD. It results in hyperphosphorylation and intraneuronal aggregation of Tau protein. In the literature, only few articles describe the role of TREM2 in the development of Tau pathology, and they report contradictory results. We therefore do not know for sure whether a deficiency in TREM2 has a deleterious effect or not on tauopathy. Thus, the goal of my thesis is to study the role of neuroinflammation and TREM2 in the progression of tauopathy, in two different models. The first is obtained by stereotaxic injection of AAV vectors into the CA1 layer of the hippocampus of TREM2-deficient or non-deficient mice. These vectors lead to the overexpression of different forms of the human tau protein, thus making it possible to recapitulate the different tauopathy stages.In parallel, we used a more progressive trangenic model of tauopathy, the THY-Tau22 mouse, to study the influence of TREM2 deficiency at different stage of the pathology. Our study demonstrated the toxicity of Tau soluble forms in the AAV model compared…

Advisors/Committee Members: Bemelmans, Alexis (thesis director).

Subjects/Keywords: Tauopathie; Neuroinflammation; Modèles in vivo; Tauopathy; Neuroinflammation; In vivo models

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vautheny, A. (2019). Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2019SACLS170

Chicago Manual of Style (16^th Edition):

Vautheny, Audrey. “Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy.” 2019. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 07, 2021. http://www.theses.fr/2019SACLS170.

MLA Handbook (7^th Edition):

Vautheny, Audrey. “Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy.” 2019. Web. 07 Mar 2021.

Vancouver:

Vautheny A. Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2019. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2019SACLS170.

Council of Science Editors:

Vautheny A. Rôle de la neuroinflammation et du récepteur microglial TREM2 dans la progression de deux modèles de tauopathie : Role of Neuroinflammation and the TREM2 Microglial Receptor in the Progression of two Models of Tauopathy. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2019. Available from: http://www.theses.fr/2019SACLS170

Uniwersytet im. Adama Mickiewicza w Poznaniu

12. Igielska-Kalwat, Joanna. Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów .

Degree: 2016, Uniwersytet im. Adama Mickiewicza w Poznaniu

URL: http://hdl.handle.net/10593/14415

► Skóra stanowi bardzo dobry model badawczy w odniesieniu do całościowego zjawiska starzenia. Jest ona bezpośrednio narażona na destrukcyjne działanie czynników zewnętrznych. Ulega starzeniu szybciej niż… (more)

Subjects/Keywords: karotenoidy; carotenoids; biodostępność; bioavailability; badania in vivo; in vivo studies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Igielska-Kalwat, J. (2016). Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów . (Doctoral Dissertation). Uniwersytet im. Adama Mickiewicza w Poznaniu. Retrieved from http://hdl.handle.net/10593/14415

Chicago Manual of Style (16^th Edition):

Igielska-Kalwat, Joanna. “Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów .” 2016. Doctoral Dissertation, Uniwersytet im. Adama Mickiewicza w Poznaniu. Accessed March 07, 2021. http://hdl.handle.net/10593/14415.

MLA Handbook (7^th Edition):

Igielska-Kalwat, Joanna. “Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów .” 2016. Web. 07 Mar 2021.

Vancouver:

Igielska-Kalwat J. Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów . [Internet] [Doctoral dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10593/14415.

Council of Science Editors:

Igielska-Kalwat J. Badanie wpływu form kosmetycznych na biodostępność wybranych karotenoidów . [Doctoral Dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2016. Available from: http://hdl.handle.net/10593/14415

University of Newcastle

13. Choi, Jeong-Hwa. In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease.

Degree: Food Science, 2014, University of Newcastle

URL: http://hdl.handle.net/1959.13/1041655

►

Research Doctorate - PhD (Food Science)

Folic acid has been the focus of extensive research since it plays an essential role in the human body… (more)

▼

Research Doctorate - PhD (Food Science)

Folic acid has been the focus of extensive research since it plays an essential role in the human body as a major coenzyme in one-carbon metabolism. This metabolic role facilitates both methionine and DNA synthesis. It has been suggested that increased folate intake, and hence blood status, provides benefits in maintaining health. However, a growing body of literature now reports differing opinions with respect to the level of folic acid intake, the type of vitamer and pathoaetiological outcomes. Part of this controversy lies in the study of cancer aetiology, particularly in the context of adenomatous polyp (AP) occurrence. AP is an antecedent of colorectal cancer. As part of the present study an in vivo model was therefore developed in order to re-examine the role of folate in carcinogenesis, taking into account both genetic variants and environmental factors. To obtain further evidence, the biological characteristics of folate vitamers in promoting cancer cell proliferation were examined using an in vitro model. 202 individuals were recruited and placed into two groups depending upon whether they had been diagnosed with AP or were control subjects. The blood folate, thiol levels, dietary folate intake, including synthetic and natural forms, and intake of nutrients related to one-carbon metabolism (pyridoxine, riboflavin, cobalamin, niacin, methionine and vitamin C) of each subject were evaluated. Sixteen major genetic variants in folate metabolism were also determined as potential risk factors in AP aetiology: MTHFR C677T, A1208C, G1793A, SHMT C1420T, TS 1496del6, TSER 2R3R, TS 3RG>C, GCPII C1561T, CBS 844ins66, DHFR 19bp del , RCF G80A, CTH G1364T, IVS 10-430 C>T, BHMT G595A, MS A2756G and MSR A66G. Statistical analysis has provided evidence to suggest that AP aetiology depends upon a range of interactions between genes and nutrition. The following factors were found to be associated with an increased risk of developing AP: RBC folate level (p for females = 0.0021 and males = 0.004), nutritional intake (p for methylfolate intake below the median = 0.0189 and cobalamin intake above median = 0.0402) and genetic variants (p for CBS 844ins68 = 0.035, TS 1496del6 = 0.048, MSR A66G = 0.025 and MTHFR C677T- CBS 844ins66 = 0.0403). Although no direct correlation was found between PteGlu intake and the occurrence of AP, genetic variants predicted a differential AP risk depending on total dietary folate intake level (in subjects with total dietary folate intake above the RDI: p for MTHFR A1298C= 0.026, MTHFR C677T-A1298C-G1793A=0.016, TS 1496del6 = 0.024, DHFR 19bp del = 0.032, and, in subjects with total dietary folate intake below the RDI: MTHFR A1298C2= 0.011, RFC G80A-GCPII C1561T3= 0.033). Additionally, vitamin C was also associated with occurrence of AP when examined by RCF G80A and GCPII C1561T genotype (p for RFC G80A AA = 0.0473, GCP II C1561T CT= 0.0276 and RFC G80A-GCPII C1561T AA/CC = 0.0026). An in vitro model utilizing both colon (Caco-2) and breast cancer (MCF 7)…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: cancer; folic acid; in vitro; in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Choi, J. (2014). In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1041655

Chicago Manual of Style (16^th Edition):

Choi, Jeong-Hwa. “In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease.” 2014. Doctoral Dissertation, University of Newcastle. Accessed March 07, 2021. http://hdl.handle.net/1959.13/1041655.

MLA Handbook (7^th Edition):

Choi, Jeong-Hwa. “In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease.” 2014. Web. 07 Mar 2021.

Vancouver:

Choi J. In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease. [Internet] [Doctoral dissertation]. University of Newcastle; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1959.13/1041655.

Council of Science Editors:

Choi J. In vitro and in vivo approaches to the examination of folate-related nutritional genetics in health and disease. [Doctoral Dissertation]. University of Newcastle; 2014. Available from: http://hdl.handle.net/1959.13/1041655

Ruhr Universität Bochum

14. Goh, Jinzhong Jeremy. Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice.

Degree: 2012, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198

► Der Hippocampus spielt eine wichtige Rolle bei der Bildung von Gedächtnis. Die vorliegende Studie dient zur Klärung der Frage nach der synaptischen Änderung, die sich… (more)

Subjects/Keywords: Elektrophysiologie; Maus; In vivo; Neuronale Plastizität; Hippocampus

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goh, J. J. (2012). Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goh, Jinzhong Jeremy. “Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice.” 2012. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goh, Jinzhong Jeremy. “Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice.” 2012. Web. 07 Mar 2021.

Vancouver:

Goh JJ. Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice. [Internet] [Thesis]. Ruhr Universität Bochum; 2012. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goh JJ. Characterization of the effects of novel object-space information on synaptic plasticity in the hippocampal CA1 sub- region of freely behaving mice. [Thesis]. Ruhr Universität Bochum; 2012. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-36198

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

15. Ziebarth, Wibke. Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo.

Degree: 2010, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823

► Das kolorektale Adenom gilt als Vorläuferläsion des kolorektalen Karzinoms, wobei die Dysregulation des APC/β- catenin-Signalweges in der Adenom-Karzinom-Sequenz ein frühes Schlüsselereignis ist. Ziel dieser Arbeit… (more)

Subjects/Keywords: Dickdarmkrebs; Adenom; In vivo; Mesalazin; Adenom / Rückbildung

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ziebarth, W. (2010). Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ziebarth, Wibke. “Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo.” 2010. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ziebarth, Wibke. “Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo.” 2010. Web. 07 Mar 2021.

Vancouver:

Ziebarth W. Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo. [Internet] [Thesis]. Ruhr Universität Bochum; 2010. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ziebarth W. Der Einfluss von 5-Aminosalicylsäure auf die Progression kolorektaler Adenome in vivo. [Thesis]. Ruhr Universität Bochum; 2010. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-28823

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

16. Buschler, Arne. Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment.

Degree: 2012, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433

► Synaptische Plastizität gilt als Mechanismus, der Lern- und Gedächtnisvorgängen zugrunde liegt. Dabei spielt auf molekularer Ebene der NMDA-Rezeptor, besonders an den Schaffer-Kollateralen-CA1-Synapsen, eine große Rolle.… (more)

Subjects/Keywords: Neuronale Plastizität; In vivo; Elektrophysiologie; Langzeitpotenzierung; Laus

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Buschler, A. (2012). Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Buschler, Arne. “Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment.” 2012. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Buschler, Arne. “Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment.” 2012. Web. 07 Mar 2021.

Vancouver:

Buschler A. Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment. [Internet] [Thesis]. Ruhr Universität Bochum; 2012. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Buschler A. Characterisation of hippocampal synaptic plasticity in freely behaving mice and its modulation by environmental enrichment. [Thesis]. Ruhr Universität Bochum; 2012. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-37433

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

17. Merkendorf, Tobias. Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui.

Degree: 2014, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178

► Mikrobielle Rhodopsine sind eine weitverbreitete Gruppe von Membranproteinen mit vielen unterschiedlichen Funktionen. Eine davon ist, lichtgetrieben Protonen über eine Membran zu transportieren und einen Protonengradienten… (more)

Subjects/Keywords: Bakteriorhodopsin; Membrantransport; Photozyklus; In vivo; Optogenetik

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Merkendorf, T. (2014). Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Merkendorf, Tobias. “Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui.” 2014. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Merkendorf, Tobias. “Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui.” 2014. Web. 07 Mar 2021.

Vancouver:

Merkendorf T. Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Merkendorf T. Biophysikalische Untersuchungen am Protonenpumpmechanismus von Rhodopsinen aus Halobacterium salinarum und Haloarcula marismortui. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Cristina Apolinário da Silva, Andréa. Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas .

Degree: 2008, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/2183

► A esquistossomose é uma doença parasitária crônica das regiões tropicais e subtropicais e está associada a uma alta morbidade da população infectada. A terapia da… (more)

▼ A esquistossomose é uma doença parasitária crônica das regiões tropicais e subtropicais e está associada a uma alta morbidade da população infectada. A terapia da esquistossomose é feita apenas com um único fármaco, o praziquantel, ativo contra todas as espécies de Schistosoma, e o oxamniquina ativo apenas contra o Schistosoma mansoni. Contudo há relatos na literatura de resistência do parasito ao tratamento para os dois fármacos, gerando uma preocupação a nível mundial e levando a Organização Mundial de Saúde (OMS) convocar a indústria e os pesquisadores a buscar novos fármacos com esta finalidade. Neste contexto a busca de novos fármacos com atividade esquistossomicida torna-se o objetivo deste trabalho, onde os derivados imidazolidínicos das séries 5-benzilideno-3-benzil-4-tioxo-imidazolidin-2-ona (LPSF/PT), 5-benzilideno-3-(4-metil-benzil)-imidazolidina-2,4-diona (LPSF/MS) e 5-arilazo-4-tioxo-imidazolidin-2-ona (LPSF/PT) foram avaliados in vitro frente a vermes adultos de Schistosoma mansoni (Cepa BH). Dos derivados avaliados in vitro os que apresentaram atividade esquistossomicida, foram os derivados da série 3-benzil-5-(arilazo)-4-tioxo-imidazolidin-2-ona (LPSF/PT). Para a avaliação in vivo, o derivado 3-benzil-5-(4-cloro-arilazo)-4-tioxoimidazolidin- 2-ona (LPSF/PT05) foi administrado oralmente em Tween 80, Tween/óleo/água e em dispersão sólida com 90 % PEG (Polietilenoglicol). Apenas esta última apresentou efeito esquistossomicida. Foram administradas quatro doses: 3, 10, 30 e 100 mg/kg. A redução do número de vermes recuperados foi significativa para a dose de 10 e 30 mg/kg, aproximadamente 50 % e para a dose de 100 mg/kg a redução foi de 68%. A redução do número de vermes na dose de 100 mg/kg corroborou com a resposta imune celular do derivado e levantou a hipótese do seu efeito imunossupressor ser ao nível de resposta Th1 e Th2. Somado a esses dados a resposta histopatológica revelou uma modulação na resposta granulomatosa, produziu uma ação contra o dano hepático causado pela infecção pelo S. mansoni Advisors/Committee Members: Lins Galdino, Suely (advisor).

Subjects/Keywords: Esquistossomose; Atividade Esquistossomicida; Imidazolidina; Avaliação in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cristina Apolinário da Silva, A. (2008). Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/2183

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cristina Apolinário da Silva, Andréa. “Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas .” 2008. Thesis, Universidade Federal de Pernambuco. Accessed March 07, 2021. http://repositorio.ufpe.br/handle/123456789/2183.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cristina Apolinário da Silva, Andréa. “Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas .” 2008. Web. 07 Mar 2021.

Vancouver:

Cristina Apolinário da Silva A. Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2008. [cited 2021 Mar 07]. Available from: http://repositorio.ufpe.br/handle/123456789/2183.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cristina Apolinário da Silva A. Novas moléculas imidazolidínicas: potenciais candidatas a fármacos esquistossomicidas . [Thesis]. Universidade Federal de Pernambuco; 2008. Available from: http://repositorio.ufpe.br/handle/123456789/2183

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Amaral, Leonardo Lira do. Desenvolvimento de uma metodologia de avaliação dosimétrica de transmissão, usando filmes radiocrômicos em tratamentos radioterápicos.

Degree: PhD, Física Aplicada à Medicina e Biologia, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/59/59135/tde-13052014-144712/ ;

►

Apesar da introdução do controle da qualidade individual nas técnicas complexas de tratamentos, tem-se comprovado que, mesmo assim, é possível a ocorrência de erros na… (more)

▼

Apesar da introdução do controle da qualidade individual nas técnicas complexas de tratamentos, tem-se comprovado que, mesmo assim, é possível a ocorrência de erros na aplicação da dose no momento da aplicação. No entanto, ainda não estão bem estabelecidas as ferramentas de redundância a fim de controlar a dose no momento da terapêutica, além do que, as técnicas mais modernas de tratamento radioterápico desenvolvem as aplicações com feixes rotacionais e os dosímetros tradicionalmente utilizados em controle da qualidade oferecem limitações angulares. Assim, este trabalho vem contribuir para o desenvolvimento de uma metodologia de controle da qualidade de transmissão in vivo utilizando filmes radiocrômicos acoplados ao cabeçote do acelerador linear, durante aplicações radioterápicas nas técnicas de tratamento conformacional e IMRT. A metodologia de controle da qualidade desenvolvida neste trabalho baseia-se na obtenção da distribuição de dose in vivo de tratamentos radioterápicos com um filme radiocrômico EBT2 posicionado em um suporte acrílico, semelhante a uma bandeja, a uma distância fonte-superfície de 56,8 cm, acoplado ao acessório holder do acelerador linear durante a aplicação de todo o tratamento teleterápico. Posteriormente, foi realizada uma análise gama para comparação da distribuição de dose medida pelo filme com a esperada pelo sistema de planejamento, obtida no plano coronal e central de um objeto simulador, com dimensões semelhantes ao suporte acrílico, posicionado à distância de 100 cm, como resultado da transferência do plano em questão. Com os resultados encontrados na seção conformacional, avaliando tanto a simulação Monte Carlo quanto as irradiações, pode-se concluir que a diferença entre a distribuição de dose do sistema de planejamento, na distância foco detector de 100 cm, e do filme, na distância de 56,8 cm, é diminuta e, desta forma, é viável criar uma metodologia para verificação dosimétrica de transmissão utilizando o filme radiocrômico acoplado ao cabeçote do acelerador. O controle da qualidade proposto na técnica de IMRT concordou com o esperado em 24 das 25 situações testadas, apresentando apenas um resultado diferente, ou seja, uma concordância de 96% com o esperado. As avaliações in vivo concordaram com 98% dos controles avaliados. Desta forma, pode-se concluir que a metodologia proposta neste trabalho é factível para o controle da qualidade de transmissão in vivo, em tratamentos radioterápicos que usam a técnica de tratamento conformacional e IMRT e, como ela não oferece dificuldades para o deslocamento angular do gantry, ela poderá ser aplicada em técnicas teleterápicas mais modernas.

Even with the introduction of the individual quality control in the complex techniques of radiation therapy treatments, the occurrence of errors in the release of the dose at the time of application is possible. However, in order to monitor the dose at the time of therapy, redundancy tools are not yet well established, Besides that, the most modern techniques of radiation treatment use rotational beams…

Advisors/Committee Members: Ghilardi Netto, Thomaz.

Subjects/Keywords: Avaliações in vivo; Controle da Qualidade; Dosimetria in vivo; Filme Radiocrômico; IMRT; IMRT; in vivo dosimetry; in vivo evaluations; quality assurance; radiochromic film

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Amaral, L. L. d. (2014). Desenvolvimento de uma metodologia de avaliação dosimétrica de transmissão, usando filmes radiocrômicos em tratamentos radioterápicos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/59/59135/tde-13052014-144712/ ;

Chicago Manual of Style (16^th Edition):

Amaral, Leonardo Lira do. “Desenvolvimento de uma metodologia de avaliação dosimétrica de transmissão, usando filmes radiocrômicos em tratamentos radioterápicos.” 2014. Doctoral Dissertation, University of São Paulo. Accessed March 07, 2021. http://www.teses.usp.br/teses/disponiveis/59/59135/tde-13052014-144712/ ;.

MLA Handbook (7^th Edition):

Amaral, Leonardo Lira do. “Desenvolvimento de uma metodologia de avaliação dosimétrica de transmissão, usando filmes radiocrômicos em tratamentos radioterápicos.” 2014. Web. 07 Mar 2021.

Vancouver:

Amaral LLd. Desenvolvimento de uma metodologia de avaliação dosimétrica de transmissão, usando filmes radiocrômicos em tratamentos radioterápicos. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Mar 07]. Available from: http://www.teses.usp.br/teses/disponiveis/59/59135/tde-13052014-144712/ ;.

Council of Science Editors:

Amaral LLd. Desenvolvimento de uma metodologia de avaliação dosimétrica de transmissão, usando filmes radiocrômicos em tratamentos radioterápicos. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/59/59135/tde-13052014-144712/ ;

University of Michigan

20. Vigen, Marina. Investigating the Role of Matrix Architecture on Vascularization in MMP-Sensitive PEG Hydrogels.

Degree: PhD, Biomedical Engineering, 2014, University of Michigan

URL: http://hdl.handle.net/2027.42/110494

► The formation of functional blood vessels in engineered or ischemic tissues remains a significant scientific and clinical hurdle. Cell delivery, scaffold design, and growth factor… (more)

▼ The formation of functional blood vessels in engineered or ischemic tissues remains a significant scientific and clinical hurdle. Cell delivery, scaffold design, and growth factor delivery have been investigated to support neovascularization. This thesis focuses on a hybrid approach wherein cells are seeded within a biosynthetic scaffold. Our approach is motivated by the relatively poor performance of cells alone; cell engraftment is minimal (10%) in scaffold-free approaches. Natural and synthetic materials have been utilized to improve engraftment, but the biosynthetic scaffold presented here offers unique advantages to overcome limitations of natural materials and offers tunability of matrix properties and biological response. A PEG hydrogel platform was adapted to investigate the roles of network crosslinking density and susceptibility to proteolysis on vascularization. Four-arm PEG vinyl sulfone (PEGVS) was polymerized by Michael-type addition with reactive cysteine groups on a slowly degraded matrix metalloprotease (MMP) susceptible peptide, GPQG↓IWGQ, or a peptide that is cleaved more rapidly, VPMS↓MRGG. Vascular networks formed in vitro from encapsulated endothelial cells and supportive stromal fibroblasts. Morphogenesis was robust to changes in cross-linking peptide identity, but significantly attenuated in more crosslinked gels. All gel types supported the de novo formation of perfused vasculature from transplanted cells in subcutaneous implants in vivo; however, unlike the in vitro findings, vascularization was not decreased in the more cross-linked gels. A mouse model of hindlimb ischemia was used to further assess the ability of PEG hydrogels to support revascularization in a model relevant for clinical translation. Cell-laden PEG hydrogel precursors and fibrin controls were delivered to SCID mice after femoral artery ligation. PEG hydrogels supported the formation of perfused vasculature irrespective of crosslinking-peptide identity. Hydrogel delivery improved reperfusion to the ischemic limb. Substantial loss of gel mechanical integrity and vessel regression were evident in fibrin gels, but not in PEG gels, 2 weeks post-implantation, suggesting PEG hydrogels are superior to fibrin with regards to vessel persistence. In sum, these findings demonstrate that structurally stable biomimetic PEG-based hydrogels direct vascularization in ischemic tissues via cell transplantation and hold promise in tissue regeneration and therapeutic angiogenesis. Advisors/Committee Members: Putnam, Andrew James (committee member), Weiss, Stephen J. (committee member), Stegemann, Jan Philip (committee member), Shikanov, Ariella (committee member).

Subjects/Keywords: vascularization; biomaterials; in vivo; Biomedical Engineering; Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vigen, M. (2014). Investigating the Role of Matrix Architecture on Vascularization in MMP-Sensitive PEG Hydrogels. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/110494

Chicago Manual of Style (16^th Edition):

Vigen, Marina. “Investigating the Role of Matrix Architecture on Vascularization in MMP-Sensitive PEG Hydrogels.” 2014. Doctoral Dissertation, University of Michigan. Accessed March 07, 2021. http://hdl.handle.net/2027.42/110494.

MLA Handbook (7^th Edition):

Vigen, Marina. “Investigating the Role of Matrix Architecture on Vascularization in MMP-Sensitive PEG Hydrogels.” 2014. Web. 07 Mar 2021.

Vancouver:

Vigen M. Investigating the Role of Matrix Architecture on Vascularization in MMP-Sensitive PEG Hydrogels. [Internet] [Doctoral dissertation]. University of Michigan; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2027.42/110494.

Council of Science Editors:

Vigen M. Investigating the Role of Matrix Architecture on Vascularization in MMP-Sensitive PEG Hydrogels. [Doctoral Dissertation]. University of Michigan; 2014. Available from: http://hdl.handle.net/2027.42/110494

Vanderbilt University

21. Miteva, Martina. Optimizing PEG Molecular Weight and Molar Composition for Enhanced In Vivo Pharmacokinetics of a Mixed Micellar siRNA Carrier.

Degree: MS, Biomedical Engineering, 2013, Vanderbilt University

URL: http://hdl.handle.net/1803/15044

► RNA interference (RNAi) by small interfering RNA (siRNA) possesses great promise as a therapeutic for pathologies whose etiology is related to gene overexpression. However, due… (more)

Subjects/Keywords: PEG; pharmacokinetics; siRNA; cationic micelles; in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miteva, M. (2013). Optimizing PEG Molecular Weight and Molar Composition for Enhanced In Vivo Pharmacokinetics of a Mixed Micellar siRNA Carrier. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miteva, Martina. “Optimizing PEG Molecular Weight and Molar Composition for Enhanced In Vivo Pharmacokinetics of a Mixed Micellar siRNA Carrier.” 2013. Thesis, Vanderbilt University. Accessed March 07, 2021. http://hdl.handle.net/1803/15044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miteva, Martina. “Optimizing PEG Molecular Weight and Molar Composition for Enhanced In Vivo Pharmacokinetics of a Mixed Micellar siRNA Carrier.” 2013. Web. 07 Mar 2021.

Vancouver:

Miteva M. Optimizing PEG Molecular Weight and Molar Composition for Enhanced In Vivo Pharmacokinetics of a Mixed Micellar siRNA Carrier. [Internet] [Thesis]. Vanderbilt University; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1803/15044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miteva M. Optimizing PEG Molecular Weight and Molar Composition for Enhanced In Vivo Pharmacokinetics of a Mixed Micellar siRNA Carrier. [Thesis]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/15044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McMaster University

22. Balenko, Matthew. CAPSAZEPINE ATTENUATES CANCER-INDUCED BONE PAIN BY INHIBITING GLUTAMATE RELEASE.

Degree: MSc, 2014, McMaster University

URL: http://hdl.handle.net/11375/15970

►

Breast cancer has the highest incidence rate in women, accounting for more than 22% of all cancers and possessing a strong disposition to metastasize to… (more)

▼

Breast cancer has the highest incidence rate in women, accounting for more than 22% of all cancers and possessing a strong disposition to metastasize to bone. These skeletal metastases become a significant cause of morbidity and mortality in patients with the primary symptom being pain. Pain is a major concern in determining a patient’s quality of life and there have been many attempts to understand and control bone pain with little success. Previous studies have shown that glutamate plays a role in bone cancer pain, with an excess in free glutamate able to cause pain either directly through excitotoxic pathways or indirectly though the dysregulation of osteoclasts and osteoblasts, causing bone dysregulation. TRPV-1 receptors have also has been implicated in the mechanisms of bone cancer pain, as osteoclasts release protons during bone remodeling which can elicit a TRPV-1-related nociceptive response from neurons in the surrounding periosteum. Capsazepine was identified during a high throughput screen of 30,000 compounds to be a potent inhibitor of breast cancer cell-mediated glutamate release, a neurotransmitter with known associations in neural signaling, bone homeostasis, and pain. Capsazepine also has antagonistic effects on transient receptor potential vanilloid type 1 (TRPV-1) receptors which act as key players in both heat and vanilloid-induced nociception. These findings suggest that Capsazepine may provide a multi-site effect for the treatment of cancer-induced bone pain. An animal model of breast cancer-induced bone pain involved intrafemorally injecting MDA-MB-231 cancer cells to measure pain. Behavioural tests are then performed measuring dynamic weight bearing and paw withdrawal thresholds. These measurements are used to demonstrate both movement-evoked and spontaneous pain-related behaviour of the affected limb. Using Capsazepine, we demonstrate a dose-dependent attenuation of pain behaviour in vivo, while confirming tumour presence using immunohistochemistry (IHC). We show that TRPV-1 and glutamate play an important role in the onset and severity of bone cancer pain and blocking these pain pathways provide relief from pain commonly associated with cancer in the bone.

Thesis

Master of Health Sciences (MSc)

Advisors/Committee Members: Singh, Gurmit, Health Sciences.

Subjects/Keywords: Glutamate; Cancer; Pain; TRPV1; Capsazepine; in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balenko, M. (2014). CAPSAZEPINE ATTENUATES CANCER-INDUCED BONE PAIN BY INHIBITING GLUTAMATE RELEASE. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/15970

Chicago Manual of Style (16^th Edition):

Balenko, Matthew. “CAPSAZEPINE ATTENUATES CANCER-INDUCED BONE PAIN BY INHIBITING GLUTAMATE RELEASE.” 2014. Masters Thesis, McMaster University. Accessed March 07, 2021. http://hdl.handle.net/11375/15970.

MLA Handbook (7^th Edition):

Balenko, Matthew. “CAPSAZEPINE ATTENUATES CANCER-INDUCED BONE PAIN BY INHIBITING GLUTAMATE RELEASE.” 2014. Web. 07 Mar 2021.

Vancouver:

Balenko M. CAPSAZEPINE ATTENUATES CANCER-INDUCED BONE PAIN BY INHIBITING GLUTAMATE RELEASE. [Internet] [Masters thesis]. McMaster University; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11375/15970.

Council of Science Editors:

Balenko M. CAPSAZEPINE ATTENUATES CANCER-INDUCED BONE PAIN BY INHIBITING GLUTAMATE RELEASE. [Masters Thesis]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/15970

Penn State University

23. Huo, Bingxing. Control of cerebral hemodynamics during voluntary locomotion in rodents.

Degree: 2014, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/23641

► Increases in cerebral blood flow are widely used as surrogate indicators of neural activity, making the understanding of the precision and regularity of cerebral hemodynamic… (more)

▼ Increases in cerebral blood flow are widely used as surrogate indicators of neural activity, making the understanding of the precision and regularity of cerebral hemodynamic signals of fundamental importance. To determine whether cerebral blood flow is tightly regulated spatially and temporally, we measured hemodynamic signals in the mouse cortex during voluntary locomotion, which drives large changes in neural activity and in the peripheral cardiovascular system. Locomotion drives the increase of neural activity in both frontal and parietal cortices in mice. Parietal cortex, especially within the forelimb and hindlimb representations, showed significant increases of blood flow and volume, supporting neurovascular coupling. But frontal cortex showed little or no hemodynamic response, suggesting a neurovascular decoupling mechanism within the region. To quantify the spatial and temporal characters of cerebral hemodynamics, we developed a novel empirical model that is based on microscopic dynamics of artery and vein dilations. This model reliably fits the cerebral hemodynamics in the parietal cortex and quantitatively separated out arterial and venous blood volume. Using this model, we showed that the cerebral hemodynamics in regions presenting neurovascular coupling are highly predictable in space and time. Further, by pharmacologically manipulating the heart rate, we separately tested the sympathetic and parasympathetic influences on the cerebral hemodynamics. We found that the cerebral arterial response and blood flow are highly robust to cardiovascular perturbations; but venous blood volume changes were largely compromised by adrenergic blockade. We concluded that neurovascular coupling during voluntary locomotion is a region-specific, rather than a global phenomenon. The arterial response within the region presenting neurovascular coupling is tightly controlled by a central nervous system, while the venous response is under the influence of the peripheral system. Advisors/Committee Members: Patrick James Drew, Dissertation Advisor/Co-Advisor, Steven Schiff, Committee Member, Corina Stefania Drapaca, Committee Member, Dezhe Jin, Committee Member.

Subjects/Keywords: cerebral hemodynamics; neurovascular coupling; in vivo imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huo, B. (2014). Control of cerebral hemodynamics during voluntary locomotion in rodents. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/23641

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huo, Bingxing. “Control of cerebral hemodynamics during voluntary locomotion in rodents.” 2014. Thesis, Penn State University. Accessed March 07, 2021. https://submit-etda.libraries.psu.edu/catalog/23641.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huo, Bingxing. “Control of cerebral hemodynamics during voluntary locomotion in rodents.” 2014. Web. 07 Mar 2021.

Vancouver:

Huo B. Control of cerebral hemodynamics during voluntary locomotion in rodents. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Mar 07]. Available from: https://submit-etda.libraries.psu.edu/catalog/23641.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huo B. Control of cerebral hemodynamics during voluntary locomotion in rodents. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/23641

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

24. Best, Gordon. Scaphoid and Lunate Carpal Mechanics Over the Spectrum of Healthy Function .

Degree: Mechanical and Materials Engineering, 2016, Queens University

URL: http://hdl.handle.net/1974/14609

► When ligaments within the wrist are damaged, the resulting loss in range of motion and grip strength can lead to reduced earning potential and restricted… (more)

Subjects/Keywords: In Vivo ; Laxity ; Biomechanics ; Scaphoid ; Lunate ; Wrist

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Best, G. (2016). Scaphoid and Lunate Carpal Mechanics Over the Spectrum of Healthy Function . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/14609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Best, Gordon. “Scaphoid and Lunate Carpal Mechanics Over the Spectrum of Healthy Function .” 2016. Thesis, Queens University. Accessed March 07, 2021. http://hdl.handle.net/1974/14609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Best, Gordon. “Scaphoid and Lunate Carpal Mechanics Over the Spectrum of Healthy Function .” 2016. Web. 07 Mar 2021.

Vancouver:

Best G. Scaphoid and Lunate Carpal Mechanics Over the Spectrum of Healthy Function . [Internet] [Thesis]. Queens University; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1974/14609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Best G. Scaphoid and Lunate Carpal Mechanics Over the Spectrum of Healthy Function . [Thesis]. Queens University; 2016. Available from: http://hdl.handle.net/1974/14609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vermont

25. Spinella, Anthony J. In vivo characterization of hippocampal electrophysiological processes in the heterozygous Pten knockout model of autism.

Degree: Neuroscience, 2018, University of Vermont

URL: https://scholarworks.uvm.edu/hcoltheses/258

► While cognitive deficits have been described in the heterozygous Pten (+/-) KO mouse model of autism, little work has been done to demonstrate how… (more)

Subjects/Keywords: Pten; Autism; CA1; Place Cells; in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Spinella, A. J. (2018). In vivo characterization of hippocampal electrophysiological processes in the heterozygous Pten knockout model of autism. (Thesis). University of Vermont. Retrieved from https://scholarworks.uvm.edu/hcoltheses/258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Spinella, Anthony J. “In vivo characterization of hippocampal electrophysiological processes in the heterozygous Pten knockout model of autism.” 2018. Thesis, University of Vermont. Accessed March 07, 2021. https://scholarworks.uvm.edu/hcoltheses/258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Spinella, Anthony J. “In vivo characterization of hippocampal electrophysiological processes in the heterozygous Pten knockout model of autism.” 2018. Web. 07 Mar 2021.

Vancouver:

Spinella AJ. In vivo characterization of hippocampal electrophysiological processes in the heterozygous Pten knockout model of autism. [Internet] [Thesis]. University of Vermont; 2018. [cited 2021 Mar 07]. Available from: https://scholarworks.uvm.edu/hcoltheses/258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Spinella AJ. In vivo characterization of hippocampal electrophysiological processes in the heterozygous Pten knockout model of autism. [Thesis]. University of Vermont; 2018. Available from: https://scholarworks.uvm.edu/hcoltheses/258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

26. Yuen, Jones. Preparation, characterisation and in vivo osteogenesis of mesoporous bioactive glasses.

Degree: 2012, Queensland University of Technology

URL: https://eprints.qut.edu.au/53101/

► Bone defects, especially large bone defects, remain a major challenge in orthopaedic surgery. Autologous bone transplantation is considered the most effective treatment, but insufficient donor… (more)

▼ Bone defects, especially large bone defects, remain a major challenge in orthopaedic surgery. Autologous bone transplantation is considered the most effective treatment, but insufficient donor tissue, coupled with concerns about donor site morbidity, has hindered this approach in large-scale applications. Alternative approaches include implanting biomaterials such as bioactive glass (BG), which has been widely used for bone defect healing, due to having generally good biocompatibility, and can be gradually biodegraded during the process of new bone formation. Mesoporous bioactive glass (MBG) is a newly developed bioactive glass which has been proven to have enhanced in-vitro bioactivity; however the in-vivo osteogenesis has not been studied. A critical problem in using the bone tissue engineering approach to restore large bone defects is that the nutrient supply and cell viability at the centre of the scaffold is severely hampered since the diffusion distance of nutrients and oxygen for cell survival is limited to 150-200µm. Cobalt ions has been shown to mimic hypoxia, which plays a pivotal role in coupling angiogenesis with osteogenesis in-vivo by activating hypoxia inducing factor-1α (HIF-1α) transcription factor, subsequently initiating the expression of genes associated with tissue regeneration. Therefore, one aim of this study is to investigate the in-vivo osteogenesis of MBG by comparison with BG and β-TCP, which are widely used clinically. The other aim is to explore hypoxia-mimicking biomaterials by incorporating Cobalt into MBG and β-TCP. MBG and β-TCP incorporated with 5% cobalt (5Co-MBG and 5CCP) have also been studied in-vivo to determine whether the hypoxic effect has a beneficial effect on the bone formation. The composition and microstructure of synthesised materials (BG, MBG, 5Co-MBG, 5CCP) were characterised, along with the mesopore properties of the MBG materials. Dissolution and cytotoxicity of the Co-containing materials were also investigated. Femoral samples with defects harvested at 4 and 8 weeks were scanned using micro-CT followed by processing for histology (H&E staining) to determine bone formation. Histology of MBG showed a slower rate of bone formation at 4 weeks than BG, however at 8 weeks it could be clearly seen that MBG had more bone formation. The in-vivo results show that the osteogenesis of MBG reciprocates the enhanced performance shown in-vitro compared to BG. Dissolution study showed that Co ions can be efficiently released from MBG and β-TCP in a controllable way. Low amounts of Co incorporated into the MBG and β-TCP showed no significant cytotoxicity and the Co-MBG powders maintained a mesopore structure although not as highly ordered as pure MBG. Preliminary study has shown that Co incorporated samples showed little to no bone formation, instead incurring high lymphocyte activity. Further studies need to be done on Co incorporated materials to determine the cause for high lymphocyte activity in-vivo, which appear to hinder bone formation. In conclusion, this…

Subjects/Keywords: in vivo osteogenesis; mesoporous bioactive glasses

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yuen, J. (2012). Preparation, characterisation and in vivo osteogenesis of mesoporous bioactive glasses. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/53101/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yuen, Jones. “Preparation, characterisation and in vivo osteogenesis of mesoporous bioactive glasses.” 2012. Thesis, Queensland University of Technology. Accessed March 07, 2021. https://eprints.qut.edu.au/53101/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yuen, Jones. “Preparation, characterisation and in vivo osteogenesis of mesoporous bioactive glasses.” 2012. Web. 07 Mar 2021.

Vancouver:

Yuen J. Preparation, characterisation and in vivo osteogenesis of mesoporous bioactive glasses. [Internet] [Thesis]. Queensland University of Technology; 2012. [cited 2021 Mar 07]. Available from: https://eprints.qut.edu.au/53101/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yuen J. Preparation, characterisation and in vivo osteogenesis of mesoporous bioactive glasses. [Thesis]. Queensland University of Technology; 2012. Available from: https://eprints.qut.edu.au/53101/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Northeastern University

27. Luo, Yi. The Development Of Nanosensors For In Vivo Detection Of Physiological Molecules.

Degree: PhD, School of Pharmacy, 2018, Northeastern University

URL: http://hdl.handle.net/2047/D20316234

► Nanosensors are an emerging tool for biomedical research and personalized medicine. By incorporating a recognition moiety and a reporter on a nanoscale platform, the nanosensor… (more)

▼ Nanosensors are an emerging tool for biomedical research and personalized medicine. By incorporating a recognition moiety and a reporter on a nanoscale platform, the nanosensor has displayed its ability to continuously monitor physiological molecules. To develop nanosensors for in vivo applications, characteristics such as sensitivity, dynamic range, selectivity, reversibility, biocompatibility, residency time, implantation, and clearance have to be considered. Choosing and optimizing the recognition moiety is crucial for the fabrication of an in vivo nanosensor. Currently, natural large molecules, such as proteins and nucleic acids, enzymes, and synthetic small molecules have all been used as recognition moieties. In this thesis, we will demonstrate two nanosensors using different recognition moieties to measure acetylcholine and glucose, respectively. First, acetylcholine is a neurotransmitter associated with cognition, learning, and memory. Previously, the detection of acetylcholine relied on microelectrode and microdialysis, which suffer from invasiveness and limited spatial resolution respectively. To overcome these issues, we developed a nanosensor to detect acetylcholine using magnetic resonance imaging (MRI). Butyrylcholinesterase (BuChE) serving as the recognition moiety, and pH-sensitive contrast agents serving as the reporter, are immobilized on the surface of a nanoparticle. Enzymatic hydrolysis of acetylcholine created a pH-drop in the microenvironment close to the surface of the nanoparticle, which was detected by the contrast agents leading to an increase in signal intensity. Delivered to the brain of rats, this nanosensor detected drug-induced release of acetylcholine. Second, we detected glucose as the first example of small molecule detection using an optode format. The level of glucose is an important parameter to determine the dose of insulin therapy. So far, the clinically used glucose monitors are all based on the enzymatic oxidation of glucose. To extend the residency time of the glucose sensor, we developed a glucose-sensitive nanofiber based on a small molecule boronic acid as the recognition moiety. By incorporating boronic acids containing an electro-withdrawing group, the nanofiber managed to continuously detect glucose at physiological pH with an improved in vivo residency time.

Subjects/Keywords: In vivo; Nanosensor; Physiological molecules; Pharmaceutical sciences

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Luo, Y. (2018). The Development Of Nanosensors For In Vivo Detection Of Physiological Molecules. (Doctoral Dissertation). Northeastern University. Retrieved from http://hdl.handle.net/2047/D20316234

Chicago Manual of Style (16^th Edition):

Luo, Yi. “The Development Of Nanosensors For In Vivo Detection Of Physiological Molecules.” 2018. Doctoral Dissertation, Northeastern University. Accessed March 07, 2021. http://hdl.handle.net/2047/D20316234.

MLA Handbook (7^th Edition):

Luo, Yi. “The Development Of Nanosensors For In Vivo Detection Of Physiological Molecules.” 2018. Web. 07 Mar 2021.

Vancouver:

Luo Y. The Development Of Nanosensors For In Vivo Detection Of Physiological Molecules. [Internet] [Doctoral dissertation]. Northeastern University; 2018. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2047/D20316234.

Council of Science Editors:

Luo Y. The Development Of Nanosensors For In Vivo Detection Of Physiological Molecules. [Doctoral Dissertation]. Northeastern University; 2018. Available from: http://hdl.handle.net/2047/D20316234

28. Hanini-Daoud, Maroua. Traitement des informations thalamiques au travers des ganglions de la base : approche électrophysiologique et optogénétique in vivo : Treatment of thalamic information through the basal ganglia : combining electrophysiology and optogenetics in vivo.

Degree: Docteur es, Neurosciences, 2016, Aix Marseille Université

URL: http://www.theses.fr/2016AIXM4109

►

Le centre médian/parafasciculaire (CM/Pf) du thalamus a récemment émergé comme un élément d'intérêt dans le contexte de la maladie de Parkinson. Ainsi le fonctionnement normal… (more)

▼

Le centre médian/parafasciculaire (CM/Pf) du thalamus a récemment émergé comme un élément d'intérêt dans le contexte de la maladie de Parkinson. Ainsi le fonctionnement normal et pathologique des GB ne peut pas être pleinement élucidé sans qu'il ne soit pris en considération. Dans ce contexte, nous avons analysé le transfert des informations thalamiques dans les GB en enregistrant, in vivo, les réponses évoquées au niveau de la structure de sortie des GB, la substantce noire pars reticulata (SNr) soit par la stimulation électrique ou optogénétique du CM/Pf. Ensuite, nous avons étudié les composantes des GB impliquées dans ces réponses en analysant les réponses évoquées par l'activation optogenetique spécifique des voies thalamo-striée, thalamo-subthalamique ou thalamo-nigrale. À la fois l'activation électrique et optogenetique du CM/Pf évoquent des réponses complexes dans la SNr qui sont composées d'une inhibition qui peut être précédée et/ou suivie d'excitations. L'inhibition et l'excitation tardive dépendent de l'activation des voies trans-striatales, alors que les premières excitations mettent en jeu les voies thalamo-subthalamique et thalamo-nigrale. Nous avons également étudié l'impact des interneurones cholinergiques du striatum ainsi que les afférences dopaminergiques sur le transfert des informations thalamiques dans les GB. Pour ce faire, nous avons enregistré les réponses évoquées au niveau des neurones de projection du striatum suite à la stimulation électrique du CM/Pf avec ou sans l'inhibition optogénétique des CINs. Nous serons alors en mesure de déterminer comment les CINs sont impliqués dans le transfert des informations thalamiques au sein des GB.

The centre median/parafascicular (CM/Pf) of the thalamus has recently emerged as a component of interest in the context of Parkinson’s disease. Thus normal and pathological dynamics of BG cannot be fully understood unless it is taken into account. Here, we analyzed the transfer of CM/Pf information through BG by recording, in vivo, the evoked responses of BG output neurons in the substantia nigra pars reticulata (SNr) to either electrical or optogenetic CM/Pf stimulations. Then, we investigated the BG components involved in these responses by analyzing the responses evoked by specific optogenetic activation of the thalamo-striatal, thalamo-subthalamic or thalamo-nigral pathways. Both electrical and optogenetic activation of CM/Pf evoke complex responses in SNr that are composed of an inhibition that can be preceded and/or followed by excitations. The inhibition and the late excitation rely on the activation of the trans-striatal pathways, whereas the early excitations involve thalamo-subthalamic and thalamo-nigral projections. We are currently analyzing whether and how the striatal cholinergic interneurons (CINs) and the dopaminergic afferent system modulate the transfer of thalamic information within the BG. For the second part of my project, we analyzed the treatment of thalamic information from CM/Pf at the level of the striatum. To do this, we…

Advisors/Committee Members: Kerkerian-Le Goff, Lydia (thesis director), Maurice, Nicolas (thesis director).

Subjects/Keywords: Ganglions de la base; Thalamus; Interneurones cholinergiques; Électrophysiologie in vivo; Optogénétique in vivo; Basal ganglia; Thalamus; Cholinergic interneurons; Electrophysiology in vivo; Optogenetics in vivo; 612

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hanini-Daoud, M. (2016). Traitement des informations thalamiques au travers des ganglions de la base : approche électrophysiologique et optogénétique in vivo : Treatment of thalamic information through the basal ganglia : combining electrophysiology and optogenetics in vivo. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2016AIXM4109

Chicago Manual of Style (16^th Edition):

Hanini-Daoud, Maroua. “Traitement des informations thalamiques au travers des ganglions de la base : approche électrophysiologique et optogénétique in vivo : Treatment of thalamic information through the basal ganglia : combining electrophysiology and optogenetics in vivo.” 2016. Doctoral Dissertation, Aix Marseille Université. Accessed March 07, 2021. http://www.theses.fr/2016AIXM4109.

MLA Handbook (7^th Edition):

Hanini-Daoud, Maroua. “Traitement des informations thalamiques au travers des ganglions de la base : approche électrophysiologique et optogénétique in vivo : Treatment of thalamic information through the basal ganglia : combining electrophysiology and optogenetics in vivo.” 2016. Web. 07 Mar 2021.

Vancouver:

Hanini-Daoud M. Traitement des informations thalamiques au travers des ganglions de la base : approche électrophysiologique et optogénétique in vivo : Treatment of thalamic information through the basal ganglia : combining electrophysiology and optogenetics in vivo. [Internet] [Doctoral dissertation]. Aix Marseille Université 2016. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2016AIXM4109.

Council of Science Editors:

Hanini-Daoud M. Traitement des informations thalamiques au travers des ganglions de la base : approche électrophysiologique et optogénétique in vivo : Treatment of thalamic information through the basal ganglia : combining electrophysiology and optogenetics in vivo. [Doctoral Dissertation]. Aix Marseille Université 2016. Available from: http://www.theses.fr/2016AIXM4109

University of Manitoba

29. Bhullar, Prabhpal Kaur. Role of connexin36 proteins in peripheral nerves during the triple response and pain.

Degree: Physiology and Pathophysiology, 2017, University of Manitoba

URL: http://hdl.handle.net/1993/32402

► Introduction: Gap junctions allow direct cell-to-cell communication and form electrical synapses between neurons primarily via the gap junction protein connexin36 (Cx36). We examined the potential… (more)

▼ Introduction: Gap junctions allow direct cell-to-cell communication and form electrical synapses between neurons primarily via the gap junction protein connexin36 (Cx36). We examined the potential contribution of Cx36 in spinal dorsal root ganglia and peripheral nerves to the classic triple response (flare, wheal, and edema) following stimulation of nociceptors. The flare reflects increased blood flow beyond the injured area and the wheal is due to changes in the vascular permeability. Studies conducted in the 1980s suggested that electrical coupling between unmyelinated C-fibers in the periphery may contribute to these responses. Cx36 is a protein allowing gap junction formation between neurons. Objectives: We aimed to quantify the contribution of Cx36-containing gap junctions to plasma extravasation responses following stimulation of nociceptors. Methods: Wild-type (WT) and Cx36 knockout (KO) mice lacking this protein in all neurons were used for studies in which application of noxious stimuli (xylene, formalin, electrical stimulation of the sciatic nerve) was performed. Extravasation of Evans Blue dye from the circulation in the limbs was monitored via spectrophotometry, laser Doppler flux and in vivo fluorescence imaging after noxious stimulation in anesthetized mice. Results: A small but non-significant difference was found between KO and WT animals in the extravasated EB dye after front-paw’s chemical stimulation with xylene. A trend for greater responses as well as a faster rate of change in the Evans Blue fluorescence was found within the first 2-5 minutes after xylene application in WT vs. KO mice. Conclusions: The enhanced early vascular response observed in this study after nociceptor stimulation in the WT animals as compared to the KO animals is consistent with earlier reports of direct electrical coupling between C-fibers and with evidence of Cx36 expression in small sensory neurons. These results suggest that Cx36 transiently contributes to the triple response. Advisors/Committee Members: Stecina, Katinka (Physiology and Pathophysiology) (supervisor), Nagy, James I (Physiology and Pathophysiology) Jackson, Michael F (Pharmacology & Therapeutics) (examiningcommittee).

Subjects/Keywords: Connexin36; Triple response; In vivo imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bhullar, P. K. (2017). Role of connexin36 proteins in peripheral nerves during the triple response and pain. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/32402

Chicago Manual of Style (16^th Edition):

Bhullar, Prabhpal Kaur. “Role of connexin36 proteins in peripheral nerves during the triple response and pain.” 2017. Masters Thesis, University of Manitoba. Accessed March 07, 2021. http://hdl.handle.net/1993/32402.

MLA Handbook (7^th Edition):

Bhullar, Prabhpal Kaur. “Role of connexin36 proteins in peripheral nerves during the triple response and pain.” 2017. Web. 07 Mar 2021.

Vancouver:

Bhullar PK. Role of connexin36 proteins in peripheral nerves during the triple response and pain. [Internet] [Masters thesis]. University of Manitoba; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1993/32402.

Council of Science Editors:

Bhullar PK. Role of connexin36 proteins in peripheral nerves during the triple response and pain. [Masters Thesis]. University of Manitoba; 2017. Available from: http://hdl.handle.net/1993/32402

University of Bath

30. Wright, Victoria Louise. The role of nicotinic acetylcholine receptors in motivated behaviour.

Degree: PhD, 2015, University of Bath

URL: https://researchportal.bath.ac.uk/en/studentthesis/the-role-of-nicotinic-acetylcholine-receptors-in-motivated-behaviour(afd6eedb-f2af-4779-b802-2e8f59e3eb13).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687374

► Understanding how memory, learning and reward work in unison to form adaptive and sometime maladaptive behaviour is at the forefront of modern neuroscience. The largest… (more)

▼ Understanding how memory, learning and reward work in unison to form adaptive and sometime maladaptive behaviour is at the forefront of modern neuroscience. The largest unmet need in treating maladaptive reward learning behaviours such as addiction is maintaining long-term abstinence and preventing relapse after re-exposure to drug-associated cues. Nicotinic acetylcholine receptors (nAChR) have been implicated in responses to drugs of abuse other than nicotine (Rahman et al., 2015) and the aim of this work was to characterise the role of α7 nAChRs in morphine reward learning using conditioned place preference (CPP). The α7 nAChR antagonist methyllycaconitine (MLA) was used to determine if these receptors contribute to specific stages of drug-paired learning, namely acquisition, expression, reconsolidation or reinstatement of morphine-CPP. In 7-8week old C57BL/6J mice MLA (4mg/kg, s.c), given 20 minutes prior to a conditioning dose of morphine (10mg/kg, i.p) or post-test trial, had no effect on the acquisition, reconsolidation or expression of morphine-CPP. However, when given 20 minutes prior to a priming dose of morphine (5mg/kg, i.p), MLA (4mg/kg, s.c) significantly inhibited drug-induced reinstatement. The mechanisms of this effect were investigated using glutamate receptor autoradiography. Changes in 2-Amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (AMPA) and N-methyl-D-aspartate (NMDA) binding were examined in mice treated with either saline or MLA at morphine reinstatement. There were no significant changes in NMDA receptor binding (using [3H]MK-801) but morphine reinstatement significantly increased [3H]AMPA binding in the CA1/2 of the ventral but not dorsal hippocampus, or in any other brain regions examined (including mPFC, nucleus accumbens, amygdala and VTA). The selective increase in the hippocampus was partially antagonised by MLA, linking α7 nAChR activation to glutamatergic synaptic plasticity in the hippocampus. Intracranial infusions of MLA into the ventral but not the dorsal hippocampus or medial prefrontal cortex blocked reinstatement to morphine-CPP in male Wistar rats.

Subjects/Keywords: 615.7; methyllycaconitine; Conditioned Place Preference; in vivo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wright, V. L. (2015). The role of nicotinic acetylcholine receptors in motivated behaviour. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/the-role-of-nicotinic-acetylcholine-receptors-in-motivated-behaviour(afd6eedb-f2af-4779-b802-2e8f59e3eb13).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687374

Chicago Manual of Style (16^th Edition):

Wright, Victoria Louise. “The role of nicotinic acetylcholine receptors in motivated behaviour.” 2015. Doctoral Dissertation, University of Bath. Accessed March 07, 2021. https://researchportal.bath.ac.uk/en/studentthesis/the-role-of-nicotinic-acetylcholine-receptors-in-motivated-behaviour(afd6eedb-f2af-4779-b802-2e8f59e3eb13).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687374.

MLA Handbook (7^th Edition):

Wright, Victoria Louise. “The role of nicotinic acetylcholine receptors in motivated behaviour.” 2015. Web. 07 Mar 2021.

Vancouver:

Wright VL. The role of nicotinic acetylcholine receptors in motivated behaviour. [Internet] [Doctoral dissertation]. University of Bath; 2015. [cited 2021 Mar 07]. Available from: https://researchportal.bath.ac.uk/en/studentthesis/the-role-of-nicotinic-acetylcholine-receptors-in-motivated-behaviour(afd6eedb-f2af-4779-b802-2e8f59e3eb13).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687374.

Council of Science Editors:

Wright VL. The role of nicotinic acetylcholine receptors in motivated behaviour. [Doctoral Dissertation]. University of Bath; 2015. Available from: https://researchportal.bath.ac.uk/en/studentthesis/the-role-of-nicotinic-acetylcholine-receptors-in-motivated-behaviour(afd6eedb-f2af-4779-b802-2e8f59e3eb13).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687374

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations