You searched for subject:(In vitro antiplasmodial activity).
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1.
Cohen Potier de Courcy, Anita.
Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures.
Degree: Docteur es, Sciences, 2012, Aix Marseille Université
URL: http://www.theses.fr/2012AIXM5503 
► L'objectif de ce travail consiste en la synthèse et l'évaluation antiparasitaire in vitro de nouveaux dérivés du thiazole et apparentés. Plusieurs stratégies de synthèse visant…
(more)
▼ L'objectif de ce travail consiste en la synthèse et l'évaluation antiparasitaire in vitro de nouveaux dérivés du thiazole et apparentés. Plusieurs stratégies de synthèse visant à une pharmacomodulation en séries mono- et polycycliques ont été étudiées : en série 2-méthyl-5-nitrothiazole, la pharmacomodulation anti-Trichomonas de la position 4 par stratégie SRN1 n'a pas permis d'améliorer l'activité déjà démontrée en série 2-méthyl-5-nitroimidazole, mais a conduit à des dérivés à activité antiproliférative in vitro, spécifique de la lignée HepG2. En série 4-arylsulfonylméthyl-2-méthylthiazole, la pharmacomodulation de la position 5, par couplage de Suzuki-Miyaura d'une part, et par arylation directe et réaction de Knoevenagel intramoléculaire d'autre part, a conduit à des dérivés mono- et polycycliques dont certains ont démontré une activité antiplasmodiale in vitro encourageante. En série 5H-thiazolo[3,2-a]pyrimidin-5-one, la réaction de double couplage de Suzuki-Miyaura a révélé l'importance du groupement phényle en position 6 pour l'activité antiplasmodiale de ces dérivés. Enfin, l'évaluation biologique in vitro de thiéno[3,2-d]pyrimidin-4(3H)-ones a permis de caractériser le pharmacophore responsable de l'activité antiplasmodiale significative de cette série. Les résultats préliminaires encourageants d'une étude mécanistique antiplasmodiale présentent l'inhibition spécifique des kinases plasmodiales comme un mécanisme d'action potentiel de ces composés.
The objective of this work consists of the synthesis and the antiparasitic in vitro evaluation of new thiazole derivatives and related structures. Several synthetic strategies aiming at the pharmacomodulation on mono- and polycyclic series have been studied: in 2-methyl-5-nitrothiazole series, the anti-Trichomonas pharmacomodulation on position 4 by SRN1 strategy did not improve the activity previously demonstrated in 2-methyl-5-nitroimidazole series, but led to derivatives displaying a selective in vitro antiproliferative activity toward the HepG2 cell line. In 4-arylsulfonylmethyl-2-methylthiazole series, the pharmacomodulation on position 5, by Suzuki-Miyaura cross-coupling reaction on the one hand, and by direct arylation and intramolecular Knoevenagel reaction on the other hand, led to mono- and polycyclic derivatives among which some displayed an encouraging in vitro antiplasmodial activity. In 5H-thiazolo[3,2-a]pyrimidin-5-one series, a double Suzuki-Miyaura cross-coupling reaction revealed that the phenyl group on position 6 contributes to the antiplasmodial effect of this series. Finally, the in vitro biological evaluation of the thieno[3,2-d]pyrimidin-4(3H)-one scaffold let to characterize the pharmacophore responsible for the significant antiplasmodial activity. Some preliminary encouraging results regarding a mechanistic antiplasmodial study show the specific inhibition of plasmodial kinases, as a potential mechanism of action of some of these compounds.
Advisors/Committee Members: Vanelle, Patrice (thesis director), Rathelot, Pascal (thesis director).
Subjects/Keywords: Thiazole; Plasmodium falciparum; Activité antiproliférative in vitro; Activité antiplasmodiale in vitro; Pharmacomodulation; Kinases plasmodiales; Srn1; Palladium; Couplage de Suzuki-Miyaura; Arylation directe; Thiazole; Plasmodium falciparum; In vitro antiproliferative activity; In vitro antiplasmodial activity; Pharmacomodulation; Plasmodial kinases; Srn1; Palladium; Suzuki-Miyaura cross-coupling; Direct arylation
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APA (6th Edition):
Cohen Potier de Courcy, A. (2012). Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2012AIXM5503
Chicago Manual of Style (16th Edition):
Cohen Potier de Courcy, Anita. “Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures.” 2012. Doctoral Dissertation, Aix Marseille Université. Accessed January 21, 2021.
http://www.theses.fr/2012AIXM5503.
MLA Handbook (7th Edition):
Cohen Potier de Courcy, Anita. “Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures.” 2012. Web. 21 Jan 2021.
Vancouver:
Cohen Potier de Courcy A. Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures. [Internet] [Doctoral dissertation]. Aix Marseille Université 2012. [cited 2021 Jan 21].
Available from: http://www.theses.fr/2012AIXM5503.
Council of Science Editors:
Cohen Potier de Courcy A. Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures. [Doctoral Dissertation]. Aix Marseille Université 2012. Available from: http://www.theses.fr/2012AIXM5503
Freie Universität Berlin
2.
Köhler, Inga.
Evaluation of ergoline derivatives with antiplasmodial activity and in vitro
bioactivity-guided fractionation of tropical medicinal plants traditionally
used as antimalarials.
Degree: 2003, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-11371
► Malaria is still the most dangerous parasitic infectious disease causing millions of deaths every year. In many countries where Malaria is endemic the traditional medical…
(more)
▼ Malaria is still the most dangerous parasitic infectious disease causing
millions of deaths every year. In many countries where Malaria is endemic the
traditional medical methods constitute a strong part in the public health care
system. Phytochemical research on medicinal plants traditionally used as
antimalarials are urgently needed. In this context we looked at several
Central American and West African medicinal plants, which are traditionally
used against malaria and/or symptoms of the disease. Analysis of all plant
species was based on literature and/or ethnobotanical studies. We produced
plant extracts of different polarity from various plant parts of eight species
from El Slavador and eleven species from Ghana, which are still used in the
Central American and West African traditional phytotherapy, All extracts were
tested for their in
vitro actvity against a Chloroquine-sensitive strain PoW
and a Chloroquine-resistent clone Dd2 of Plasmodium falciparum. The stem bark
from Exostema mexicanum Gray (Rubiaceae), the leaves from Calea tenuifolia
Kunth (Asteraceae), and the aerial parts from Microglossa pyrifolia (Lam.)
Kuntze (Asteraceae) exhibited a promising
antiplasmodial activity. Thus
bioactivity-guided fractionations were carried out in order to isolate and
characterize the major antiprotozoan principles of this species. Further
purification of the active lipophilic extract (petrol ether/ methanol 1:1) and
the methanolic extract of E. mexicanum led to the isolation of seven
4-phenylcoumarines, with 4',5,7,8-tetramethoxy-4-phenylcoumarin [O-methyl-
exostemin], 4',8-dihydroxy-5,7-dimethoxy-4-phenylcoumarin [exomexin A], and
3',4'-dihydroxy-5,7,8-trimethoxy-4-phenylcoumarin [exomexin B] as new natural
compounds. O-Methyl-exostemin showed a significant
activity against P.
falciparum (IC50-values: 10.5 µM [PoW] and 4.7 µM [Dd2]). Additionally, of all
active compounds the cytotoxic effect on the human endothelium cell line (ECV
304) in
vitro were assessed. Here, all tested 4-phenylcoumarines exhibited a
significant cytotoxic
activity with IC50-values between 1,2 µM and 10,0 µM.
Given this result, the unrestricted use of the E. mexicanum as an
ethnomedicinal substance cannot be recommended based. Bioactivity-guided
fractionation of the lipophilic leaf extract of C. tenuifolia led to the
isolation of five flavones and two unsaturated fatty acids. All compounds
showed activities against P. falciparum, with 7-methylacacetin exhibiting the
strongest inhibition of the parasite (IC50-values: 20.1 µM [PoW] and 17.1 µM
[Dd2]). All seven compounds were also tested on the human endothelium cell
line (ECV 304). Based on our results, we cannot completely assess the
antiplasmodial potential of this plant species. However our findings may
represent a rational explanation for a potential antimalarial effect of the
leaves of C. tenuifolia. Bioactivity-guided fractionation of the aerial parts
of M. pyrifolia lead to the isolation of 18 natural compounds, e.g. different
furanoditerpenes and geranylgeraniol derivatives. Sinapyl…
Advisors/Committee Members: n (gender), Prof. Eckart Eich (firstReferee), Prof. Martin Melzig (furtherReferee).
Subjects/Keywords: in vitro antiplasmodial activity; Plasmodium falciparum; malaria; medicinal plants; ergoline derivatives; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
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APA (6th Edition):
Köhler, I. (2003). Evaluation of ergoline derivatives with antiplasmodial activity and in vitro
bioactivity-guided fractionation of tropical medicinal plants traditionally
used as antimalarials. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-11371
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Köhler, Inga. “Evaluation of ergoline derivatives with antiplasmodial activity and in vitro
bioactivity-guided fractionation of tropical medicinal plants traditionally
used as antimalarials.” 2003. Thesis, Freie Universität Berlin. Accessed January 21, 2021.
http://dx.doi.org/10.17169/refubium-11371.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Köhler, Inga. “Evaluation of ergoline derivatives with antiplasmodial activity and in vitro
bioactivity-guided fractionation of tropical medicinal plants traditionally
used as antimalarials.” 2003. Web. 21 Jan 2021.
Vancouver:
Köhler I. Evaluation of ergoline derivatives with antiplasmodial activity and in vitro
bioactivity-guided fractionation of tropical medicinal plants traditionally
used as antimalarials. [Internet] [Thesis]. Freie Universität Berlin; 2003. [cited 2021 Jan 21].
Available from: http://dx.doi.org/10.17169/refubium-11371.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Köhler I. Evaluation of ergoline derivatives with antiplasmodial activity and in vitro
bioactivity-guided fractionation of tropical medicinal plants traditionally
used as antimalarials. [Thesis]. Freie Universität Berlin; 2003. Available from: http://dx.doi.org/10.17169/refubium-11371
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Addis Ababa University
3.
ABDU, TUHA.
SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
.
Degree: 2012, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/6214
► Malaria and leishmania are very common parasitic diseases of the developing world and drug resistance has hindered their efficient control. Pyrazole derivatives were synthesized using…
(more)
▼ Malaria and leishmania are very common parasitic diseases of the developing world and drug
resistance has hindered their efficient control. Pyrazole derivatives were synthesized using
aldol condensation and subsequent cyclization reactions. The compounds were synthesized in
a good yield (71.39%-95.23%). The compounds were purified by recrystallization and their
chemical structure was characterized by elemental microanalysis, IR, and 1HNMR
spectroscopy. In vivo antimalarial and in
vitro antileishmanial
activity was conducted using
four day suppression test and Alamar blue reduction method, respectively.
The results for antimalarial
activity conducted using P. berghei infected mice at a dose level
of 48.46μmol/kg/day showed that all the synthesized compounds have lower
activity than the
standard drug chloroquine phosphate. Compound IIc, 1-phenyl-4-(3-(thiophen-2-yl)-4,5-
dihydro-1H-pyrazol-5-yl)-3-p-tolyl-1H-pyrazole, showed relatively the highest %
suppression, 63.40%. The result for antileishmanial
activity test revealed that all the
synthesized compounds except compound IIb had better antileishmanial
activity than the
standard drug miltefosine (IC50= 3.1911 μg/ml). All of the synthesized compounds except
compounds III and IIIb exhibited lower antileishmanial
activity compared to the standard
drug amphotericin B deoxycholate (IC50=0.0460 μg/ml). Compound IIIb, phenyl pyrazoline
with propanoyl side chain, 1-(3-phenyl-5-(1-phenyl-3-p-tolyl-1H-pyrazol-4-yl)-4,5-
dihydropyrazol-1-yl)propan-1-one, was found to be the most active (IC50= 0.0112) and two
hundred eighty five and four fold more active than the standards miltefosine and
amphotericin B deoxycholate, respectively.
Keywords: pyrazole, in vivo antimalarial
activity, in
vitro antileishmanial
activity.
Advisors/Committee Members: ADNAN A. BEKHIT(Prof.),(PHD) (advisor).
Subjects/Keywords: pyrazole; in vivo antimalarial activity; in vitro antileishmanial activity
Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
ABDU, T. (2012). SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6214
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
ABDU, TUHA. “SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
.” 2012. Thesis, Addis Ababa University. Accessed January 21, 2021.
http://etd.aau.edu.et/dspace/handle/123456789/6214.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
ABDU, TUHA. “SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
.” 2012. Web. 21 Jan 2021.
Vancouver:
ABDU T. SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
. [Internet] [Thesis]. Addis Ababa University; 2012. [cited 2021 Jan 21].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/6214.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
ABDU T. SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
. [Thesis]. Addis Ababa University; 2012. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6214
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Addis Ababa University
4.
ABDU, TUHA.
SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
.
Degree: 2012, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/6218
► Malaria and leishmania are very common parasitic diseases of the developing world and drug resistance has hindered their efficient control. Pyrazole derivatives were synthesized using…
(more)
▼ Malaria and leishmania are very common parasitic diseases of the developing world and drug
resistance has hindered their efficient control. Pyrazole derivatives were synthesized using
aldol condensation and subsequent cyclization reactions. The compounds were synthesized in
a good yield (71.39%-95.23%). The compounds were purified by recrystallization and their
chemical structure was characterized by elemental microanalysis, IR, and 1HNMR
spectroscopy. In vivo antimalarial and in
vitro antileishmanial
activity was conducted using
four day suppression test and Alamar blue reduction method, respectively.
The results for antimalarial
activity conducted using P. berghei infected mice at a dose level
of 48.46μmol/kg/day showed that all the synthesized compounds have lower
activity than the
standard drug chloroquine phosphate. Compound IIc, 1-phenyl-4-(3-(thiophen-2-yl)-4,5-
dihydro-1H-pyrazol-5-yl)-3-p-tolyl-1H-pyrazole, showed relatively the highest %
suppression, 63.40%. The result for antileishmanial
activity test revealed that all the
synthesized compounds except compound IIb had better antileishmanial
activity than the
standard drug miltefosine (IC50= 3.1911 μg/ml). All of the synthesized compounds except
compounds III and IIIb exhibited lower antileishmanial
activity compared to the standard
drug amphotericin B deoxycholate (IC50=0.0460 μg/ml). Compound IIIb, phenyl pyrazoline
with propanoyl side chain, 1-(3-phenyl-5-(1-phenyl-3-p-tolyl-1H-pyrazol-4-yl)-4,5-
dihydropyrazol-1-yl)propan-1-one, was found to be the most active (IC50= 0.0112) and two
hundred eighty five and four fold more active than the standards miltefosine and
amphotericin B deoxycholate, respectively.
Keywords: pyrazole, in vivo antimalarial
activity, in
vitro antileishmanial
activity.
Advisors/Committee Members: Adnan A. Bekhit, (Ph.D.) (advisor).
Subjects/Keywords: pyrazole; in vivo antimalarial activity; in vitro antileishmanial activity.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
ABDU, T. (2012). SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6218
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
ABDU, TUHA. “SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
.” 2012. Thesis, Addis Ababa University. Accessed January 21, 2021.
http://etd.aau.edu.et/dspace/handle/123456789/6218.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
ABDU, TUHA. “SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
.” 2012. Web. 21 Jan 2021.
Vancouver:
ABDU T. SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
. [Internet] [Thesis]. Addis Ababa University; 2012. [cited 2021 Jan 21].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/6218.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
ABDU T. SYNTHESIS AND BIOLOGICAL SCREENING OF SOME PYRAZOLE DERIVATIVES AS ANTIMALARIAL AND ANTILEISHMANIAL AGENTS
. [Thesis]. Addis Ababa University; 2012. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6218
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
5.
Magoshi, Innocentia
Botlhale.
Effect of in
vitro simulated gastro-duodenal digestion on the antioxidant and
anti-inflammatory activity of South Africa Fynbos honey.
Degree: MSc, Anatomy, 2017, University of Pretoria
URL: http://hdl.handle.net/2263/61661
► Honey has been shown to have bioactivity. Fynbos (FB) honey was investigated for its bioactivity as this vegetation type is from a unique bio diverse…
(more)
▼ Honey has been shown to have bioactivity. Fynbos (FB)
honey was investigated for its bioactivity as this vegetation type
is from a unique bio diverse region in the Cape Floristic Kingdom.
Six FB and one medical grade Manuka (MAN) UMF 15+ honeys that were
of quality grade (Codex Alimentarius) were used. Each honey sample
was subjected to in
vitro simulated gastro-duodenal digestion and
the antioxidant and anti-inflammatory
activity of each fraction was
determined. These fractions were undigested/raw honey (UD), gastric
digest (GD) and gastro-duodenal digest (GDD). Included were pH and
digestive enzyme controls. The total polyphenol and the flavonoid
content (TPC and TFC) were determined with the Folin-Ciocalteu
(F-C) and aluminium chloride methods respectively. Antioxidant
activity was measured with the trolox equivalent antioxidant
capacity (TEAC) and oxygen radical absorbance capacity (ORAC)
assays. Cellular antioxidant
activity (CAA) in the Caco-2 and SC-1
cell lines using the dichloroflourescein diacetate (DCFH DA) assay
was investigated. Nitric oxide (NO) scavenging
activity was
determined with the sodium nitroprusside (SNP) assay.
Pro-inflammatory and anti-inflammatory effects of honey were
evaluated in non-stimulated and stimulated with LPS/IFN γ murine
macrophage RAW 264.7 cells, respectively. Cell viability using the
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
assay was done. TPC and TFC of MAN were higher than that of FB
honeys. With GD, TPC and TFC of MAN increased and following GDD,
TPC decreased and TFC remained unchanged. In contrast TPC and TFC
of FB honey were maintained with GD and GDD. TEAC assay revealed
activity by MAN being higher than that of FB honeys. With GD
digestion, the antioxidant
activity of MAN was unchanged but
following GDD,
activity was reduced. For FB honeys, TEAC was
maintained with GD and GDD. ORAC assay revealed that the
activity
of MAN was similar to that of FB. Digestion had no effect on
activity of both MAN and FB honeys. CAA in the Caco-2 and SC-1 cell
line was higher for MAN compared to FB honey. In both cell lines a
similar trend was observed where with GD, CAA was unchanged while
with GDD, CAA was reduced. This loss of CAA following GDD was found
to be due to H2O2 formation as a result of polyphenol degradation
in an alkaline environment containing sodium bicarbonate and
pancreatin. NO scavenging
activity of MAN was greater than FB. For
both types of honey with GD, NO scavenging
activity was unchanged
and with GDD for MAN was reduced and for FB unchanged. Digestion
showed an increased pro-inflammatory effect for MAN, FB1, FB2 and
FB3. The UD fractions of MAN, FB1 and FB6 had anti-inflammatory
effects. FB5 and FB6 honeys showed increased anti-inflammatory
activity after GD and GDD. All honey fractions did not show any
cytotoxicity. In conclusion, FB honey has antioxidant, pro- and
anti-inflammatory properties. With digestion, GD
activity was
either increased or unchanged while with GDD
activity was reduced,
lost or unchanged. Observed effects were…
Advisors/Committee Members: Bester, Megan J. (advisor), Serem, June Cheptoo (coadvisor).
Subjects/Keywords: UCTD; Antioxidant
activity;
Anti-inflammatory activity; In vitro
simulated digestion;
Fynbos
Record Details
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Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Magoshi, I. (2017). Effect of in
vitro simulated gastro-duodenal digestion on the antioxidant and
anti-inflammatory activity of South Africa Fynbos honey. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/61661
Chicago Manual of Style (16th Edition):
Magoshi, Innocentia. “Effect of in
vitro simulated gastro-duodenal digestion on the antioxidant and
anti-inflammatory activity of South Africa Fynbos honey.” 2017. Masters Thesis, University of Pretoria. Accessed January 21, 2021.
http://hdl.handle.net/2263/61661.
MLA Handbook (7th Edition):
Magoshi, Innocentia. “Effect of in
vitro simulated gastro-duodenal digestion on the antioxidant and
anti-inflammatory activity of South Africa Fynbos honey.” 2017. Web. 21 Jan 2021.
Vancouver:
Magoshi I. Effect of in
vitro simulated gastro-duodenal digestion on the antioxidant and
anti-inflammatory activity of South Africa Fynbos honey. [Internet] [Masters thesis]. University of Pretoria; 2017. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/2263/61661.
Council of Science Editors:
Magoshi I. Effect of in
vitro simulated gastro-duodenal digestion on the antioxidant and
anti-inflammatory activity of South Africa Fynbos honey. [Masters Thesis]. University of Pretoria; 2017. Available from: http://hdl.handle.net/2263/61661
North-West University
6.
Vlok, Martha Carolina.
Artemisinin-quinoline hybrids :|bdesign, synthesis and antimalarial activity / Martha Carolina (Marli) Vlok
.
Degree: 2013, North-West University
URL: http://hdl.handle.net/10394/9543
► Introduction - Malaria is a major global health problem, with more than 500 million reported cases and at least 1 million deaths each year. The…
(more)
▼ Introduction -
Malaria is a major global health problem, with more than 500 million reported cases and at least 1 million deaths each year. The main problem with malaria control is the emerging drug resistance. Plasmodium falciparum (P. falciparum) developed widespread resistance to antimalarial drugs such as chloroquine (CQ) and mefloquine, but not to the artemisinins. The World Health Organization (WHO) recommended artemisinin combination therapy (ACT) for the treatment of uncomplicated malaria in all chloroquine resistance areas. However, P. falciparum has recently started to display resistance to these ACTs, highlighting the need for new chemotherapeutic approaches for the treatment of P. falciparum infections.
Aims -
The aims of this study were: (i) to design and synthesise a new series of antimalarial hybrid drugs, consisting of dihydroartemisinin (DHA) and aminoquinoline moieties bound covalently through different, very distinctive linkers; (ii) to determine the in vitro antiplasmodial activity and cytotoxicity of the synthesised series; (iii) to ascertain whether the in vitro antiplasmodial activity of the promising compounds would be carried over in vivo against Plasmodium vinckei (P. vinckei); and, (iv) to obtain an indication of the pharmacokinetic properties of this class of antimalarial drugs by performing snapshot pharmacokinetic analysis.
Methods -
DHA was coupled via an aminoethylether bond to various aminoquinolines to give hybrids and hybrid-dimers. CQ-susceptible (D10 and 3D7) and CQ-resistant (Dd2) strains of P. falciparum were used to determine the in vitro antiplasmodial activity. In vitro cytotoxicity was assessed using a mammalian cell-line (Chinese Hamster Ovarian, CHO). The antiproliferative activity of the hybrid-dimers was tested against three cell lines; renal adenocarcinoma (TK-10), breast adenocarcinoma (MCF-7) and melanoma (UACC-62). P. vinckei-infected mice were treated with the hybrid drugs for four days at a dosage of 0.8 mg/kg, 2.5 mg/kg, 7.5 mg/kg or 15 mg/kg intraperitoneally (ip) or orally (po), with 2.7 mg/kg, 8.3 mg/kg, 25 mg/kg or 50 mg/kg, in order to determine their antimalarial activity. A snapshot oral and intravenous (IV) pharmacokinetic study was performed.
Results -
All compounds were obtained as the 10-β-isomers and were isolated as the oxalate salts. Low nanomolar in vitro antiplasmodial activities were displayed by several compounds in this series, with IC50 values ranging from 5.15 to 29.5 nM, in comparison with the values of 2.09–5.11 nM and 21.54–157.90 nM for each of DHA and CQ respectively. All compounds displayed good selectivity towards P. falciparum in vitro (selectivity index (SI) ≥ 20). Two of the hybrids, featuring non-methylated and methylated two-carbon diaminoalkyl linkers, exerted potent in vivo antimalarial activities, with ED50 values of 1.1 and 1.4 mg/kg by ip route and 12 and 16 mg/kg po, respectively. Long-term monitoring of parasitaemia showed a complete cure of mice (without recrudescence) at 15 mg/kg ip and at 50 mg/kg po for these…
Subjects/Keywords: Malaria;
Artemisinin;
Quinoline;
Hybrid;
Pharmacokinetics;
In vitro and in vivo activity
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APA (6th Edition):
Vlok, M. C. (2013). Artemisinin-quinoline hybrids :|bdesign, synthesis and antimalarial activity / Martha Carolina (Marli) Vlok
. (Thesis). North-West University. Retrieved from http://hdl.handle.net/10394/9543
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vlok, Martha Carolina. “Artemisinin-quinoline hybrids :|bdesign, synthesis and antimalarial activity / Martha Carolina (Marli) Vlok
.” 2013. Thesis, North-West University. Accessed January 21, 2021.
http://hdl.handle.net/10394/9543.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vlok, Martha Carolina. “Artemisinin-quinoline hybrids :|bdesign, synthesis and antimalarial activity / Martha Carolina (Marli) Vlok
.” 2013. Web. 21 Jan 2021.
Vancouver:
Vlok MC. Artemisinin-quinoline hybrids :|bdesign, synthesis and antimalarial activity / Martha Carolina (Marli) Vlok
. [Internet] [Thesis]. North-West University; 2013. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10394/9543.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vlok MC. Artemisinin-quinoline hybrids :|bdesign, synthesis and antimalarial activity / Martha Carolina (Marli) Vlok
. [Thesis]. North-West University; 2013. Available from: http://hdl.handle.net/10394/9543
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
ÖNLÜ, Şeyma.
Hypericum türlerinde in vitro bitki rejenerasyonu ve sekonder metabolit üretimi: In vitro plant regeneration and secondary metabolite production in Hypericum species.
Degree: Ziraat Fakültesi, 2019, University of Ankara
URL: http://hdl.handle.net/20.500.12575/70195
► Hypericum cinsi içerdiği biyoaktif bileşiklerden dolayı tıbbi ve ekonomik olarak önemli bir bitki grubudur. Bu tez çalışmasında da Hypericum perforatum ve Türkiye'de sınırlı bölgelerde yetişmekte…
(more)
▼ Hypericum cinsi içerdiği biyoaktif bileşiklerden dolayı tıbbi ve ekonomik olarak önemli bir bitki grubudur. Bu tez çalışmasında da Hypericum perforatum ve Türkiye'de sınırlı bölgelerde yetişmekte olan H. pruinatum ile endemik H. heterophyllum türlerinde in
vitro sürgün rejenerasyonu, kallus eldesi ve dış koşullara alıştırma çalışmaları yürütülmüştür. H. heterophyllum türünde, 0.5 mg/L Thidiazuron (TDZ) ve 0.5 mg/L Indol Bütirik Asit (IBA) içeren Linsmaier ve Skoog (LS) besin ortamında yeşil, kırılgan ve sulu kallus elde edilmiştir. Üç türde de en yüksek sürgün rejenerasyonu 1 mg/L benzilaminopürin (BAP) içeren LS ortamından elde edilmiştir. Eksplant başına en yüksek sürgün sayısı H. perforatum türünde 9.77, H. pruinatum'da 3.20 ve H. heterophyllum türünde ise 5.30 adet olmuştur. H. pruinatum türünde in
vitro şartlarda elde edilen bitkicikler dış koşullara başarılı bir şekilde adapte edilirken, H. heterophyllum türüne ait bitkiciklerin adaptasyonunda başarı sağlanamamıştır. Ayrıca, in
vitro şartlarda gelişen sürgün ve kalluslar absisik asit (ABA) ve salisilik asit (SA) elisitörleri ile muamele edilmiştir. Doğadan (in vivo) toplanan bitkilerde, elisitasyon uygulanan in
vitro sürgün ve kalluslar ile dış koşullara adapte edilen in
vitro bitkiciklerde toplam fenolik içeriği, 2,2-difenil-1-pikrilhidrazil (DPPH) aktivitesi ve Yüksek Performanslı Sıvı Kromatografisi (YPSK) analizleri yapılmıştır. Toplam fenolik ve DPPH çalışmalarında, üç türde de doğadan toplanan bitkilerin yaprak örneklerinde en yüksek sonuçlar elde edilmiştir. Öte yandan, Hypericum cinsinin önemli bileşiklerinden olan hiperisin ve psödohiperisin H. perforatum türünün serada yetiştirilen bitkilerinin yaprak kısımlarında en yüksek seviyede tespit edilmiştir. H. pruinatum türünde hiperisin, psödohiperisin, rutin, klorojenik asit, hiperozit, kateşin, kersetin, kersitrin, kemferol ve apigenin bileşikleri YPSK analizlerinde belirlenmiştir. Hem H. pruinatum hem de H. perforatum türünde 0.05 mg/L ABA elisitör uygulamasında psödohiperisin miktarında artış meydana gelmiştir. Ayrıca, H. heterophyllum türünde klorojenik asit, hiperozit, kateşin, kersetin, kemferol ve apigenin biyoaktif bileşikleri de tespit edilmiştir.
Advisors/Committee Members: ÖZCAN,Sebahattin (advisor).
Subjects/Keywords: H.perforatum; H.pruinatum; In vitro rejenerasyon; Activity; ABA; SA
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
ÖNLÜ, . (2019). Hypericum türlerinde in vitro bitki rejenerasyonu ve sekonder metabolit üretimi: In vitro plant regeneration and secondary metabolite production in Hypericum species. (Doctoral Dissertation). University of Ankara. Retrieved from http://hdl.handle.net/20.500.12575/70195
Chicago Manual of Style (16th Edition):
ÖNLÜ, Şeyma. “Hypericum türlerinde in vitro bitki rejenerasyonu ve sekonder metabolit üretimi: In vitro plant regeneration and secondary metabolite production in Hypericum species.” 2019. Doctoral Dissertation, University of Ankara. Accessed January 21, 2021.
http://hdl.handle.net/20.500.12575/70195.
MLA Handbook (7th Edition):
ÖNLÜ, Şeyma. “Hypericum türlerinde in vitro bitki rejenerasyonu ve sekonder metabolit üretimi: In vitro plant regeneration and secondary metabolite production in Hypericum species.” 2019. Web. 21 Jan 2021.
Vancouver:
ÖNLÜ . Hypericum türlerinde in vitro bitki rejenerasyonu ve sekonder metabolit üretimi: In vitro plant regeneration and secondary metabolite production in Hypericum species. [Internet] [Doctoral dissertation]. University of Ankara; 2019. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/20.500.12575/70195.
Council of Science Editors:
ÖNLÜ . Hypericum türlerinde in vitro bitki rejenerasyonu ve sekonder metabolit üretimi: In vitro plant regeneration and secondary metabolite production in Hypericum species. [Doctoral Dissertation]. University of Ankara; 2019. Available from: http://hdl.handle.net/20.500.12575/70195
8.
Zarlaha, Aggeliki.
Απομόνωση, μελέτη και βιολογική δραστικότητα συστατικών φυσικών προϊόντων.
Degree: 2016, University of Ioannina; Πανεπιστήμιο Ιωαννίνων
URL: http://hdl.handle.net/10442/hedi/38936
► The aim of the thesis is to qualitatively and quantitatively assess the phenolic and flavonoid content of extracts of medicinal plants of the Lamiaceae family…
(more)
▼ The aim of the thesis is to qualitatively and quantitatively assess the phenolic and flavonoid content of extracts of medicinal plants of the Lamiaceae family typically found in the Greek flora. Their antioxidant capacity was correlated to their anticancer activity by in vitro and in silico studies.Specifically, we evaluated the impact of using four different solvent media (water, ethanol, dichloromethane and hexane) to the: total phenolic content, total flavonoid content, antioxidant activity, qualitative and quantitative composition of three aromatic plants: Sweet Basil (Ocimum basilicum), Rosmary (Rosmarinus officinalis Linn.), and Marjorma (Origanum Majorana).Our results showed significant differences among the four different extracting routes with ethanol being the best extracting medium. We also found that antioxidant activity is linearly correlated to the total phenolic and flavonoid content. The strong influence of the solvent medium was also confirmed by experiments with HPLC, ESI-MS and LC-MS. These experiments allowed us to determine the specific composition of each extract and the relative content for each solvent. Using these techniques we were able to suggest the content of isoeugenol in basil extracts for the first time in the literature.The biological activity of the extracts was studied in vitro against a variety of malignant and normal cells lines to assess the possible selective anticancer capacity of the extracts. The results were compared with the analogous stemmed in parallel for the main molecular constituents (eugenol, isoeugenol, linalool, caffeic acid and rosmarinic acid). Remarkably, caffeic acid was found to be in the same activity range compared to cisplatin against four cell lines exhibiting significant anticancer activity; isoeugenol was found to be more cytotoxic than eugenol. In silico modelling showed that isoeugenol can effectively inhibit cyclooxygenase and lipoxygenase enzymatic action adopting a pose similar to the co-crystallized ligand of indomethacin.
O στόχος της παρούσας διατριβής αυτής ήταν ο προσδιορισμός του είδους και της ποσότητας των φαινολικών και φλαβονοειδών ενώσεων στα εκχυλίσματα των αρωματικών και φαρμακευτικών φυτών της οικογένειας Lamiaceae της ελληνικής υπαίθρου, η διαπίστωση των αντιοξειδωτικών και αντικαρκινικών τους ιδιοτήτων και η ταυτοποίηση των ενώσεων με αντιοξειδωτική και βιολογική δράση.Σκοπός μας ήταν η μελέτη της επίδρασης τεσσάρων διαλυτών εκχύλισης (αιθανόλης, νερού, διχλωρομεθανίου και εξανίου) στο ολικό φαινολικό περιεχόμενο, στον υπολογισμό των συνολικών φλαβονοειδών, στην αντιοξειδωτική δράση, στην ποιοτική και ποσοτική σύσταση των εκχυλισμάτων τριών φυτών της ελληνικής χλωρίδας, του βασιλικού (λατ. Ocimum basilicum), του δενδρολίβανου (Rosmarinus officinalis Linn.), και της ματζουράνας (λατ. Origanum Majorana).Η σύγκριση των αποτελεσμάτων στους τέσσερις διαλύτες εκχύλισης έδειξε σημαντικές διαφορές μεταξύ αυτών, με την αιθανόλη να παρουσιάζει την καλύτερη απόδοση σε σχέση με τους υπόλοιπους διαλύτες. Οι τιμές της αντιοξειδωτικής ικανότητας…
Subjects/Keywords: Φαρμακευτικά φυτά; Αντικαρκινική δράση; Medicinal plants; In vitro anticancer activity
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APA ·
Chicago ·
MLA ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Zarlaha, A. (2016). Απομόνωση, μελέτη και βιολογική δραστικότητα συστατικών φυσικών προϊόντων. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/38936
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zarlaha, Aggeliki. “Απομόνωση, μελέτη και βιολογική δραστικότητα συστατικών φυσικών προϊόντων.” 2016. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed January 21, 2021.
http://hdl.handle.net/10442/hedi/38936.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zarlaha, Aggeliki. “Απομόνωση, μελέτη και βιολογική δραστικότητα συστατικών φυσικών προϊόντων.” 2016. Web. 21 Jan 2021.
Vancouver:
Zarlaha A. Απομόνωση, μελέτη και βιολογική δραστικότητα συστατικών φυσικών προϊόντων. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2016. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10442/hedi/38936.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zarlaha A. Απομόνωση, μελέτη και βιολογική δραστικότητα συστατικών φυσικών προϊόντων. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2016. Available from: http://hdl.handle.net/10442/hedi/38936
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
9.
Wittner, Moritz Stefan.
Anti-cariogenic Properties of Different Probiotic Bacteria in vitro.
Degree: 2020, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-27486
► Objectives: Probiotics can be used for prevention and treatment of caries. The aim was to evaluate characteristics which are relevant for the prevention of caries…
(more)
▼ Objectives: Probiotics can be used for prevention and treatment of caries. The aim was to evaluate characteristics which are relevant for the prevention of caries (arginine-deiminase-system (ADS)-
activity, cariogenicity, adhesion) from different probiotic strains.
Methods: Six lactobacilli (Lactobacillus acidophilus LA-5, Lactobacillus paracasei F-19, Lactobacillus rhamnosus GG und LB 21, Lactobacillus reuteri DSM 17928 und PTA 5289), two streptococci (Streptococcus sanguinis, Streptococcus oligofermentans) and one bifidobacterium strain (Bifidobacterium animalis subsp. Lactis BB-12) were selected. The ADS-
activity was assessed by measuring the production of L-Citrullin after incubation of the bacterial strains in the presence of galactose and arginine. Cariogenicity was evaluated using a fermentation assay with saccharose in microtiter plates with a pH value determination. The adherence characteristics were determined by measuring the amount of bacteria adhered to saliva coated hydroxyapatite. Streptococcus mutans strain ATCC 25175 was used as the positive control for bacterial adhesion and fermentation. Differences between the strains were evaluated using ANOVA and post-hoc Tukey´s HSD test.
Results: The evaluated parameters varied widely between the different bacterial strains. Lactobacillus reuteri PTA 5289 had a significantly higher ADS-
activity than all other groups, followed by Streptococcus oligofermentans (p<0,05/HSD). Furthermore, both showed no pH drop after incubation with saccharose. In the fermentation assays with Lactobacillus rhamnosus LGG and Lactobacillus paracasei F-19 pH dropped under a critical value of 5.2. Streptococcus oligofermentans showed the significantly highest adhesion capacity of all the tested strains. The adhesion of all lactobacilli and the bifidobacterium strains was lower in comparison to the streptococci strains (p<0,05)
Conclusion: Streptococcus oligofermentans and Lactobacillus reuteri PTA 5289 have promising characteristics for the prevention of caries. Further studies should aim to investigate the influence of these strains on cariogenic biofilms and the demineralization of dental hard tissue.
Advisors/Committee Members: male (gender), N.N. (firstReferee), N.N. (furtherReferee).
Subjects/Keywords: probiotics; arginine-deaminase-system activity; in vitro; adhesion; cariogenicity; ddc:610
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APA ·
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Manager
APA (6th Edition):
Wittner, M. S. (2020). Anti-cariogenic Properties of Different Probiotic Bacteria in vitro. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27486
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wittner, Moritz Stefan. “Anti-cariogenic Properties of Different Probiotic Bacteria in vitro.” 2020. Thesis, Freie Universität Berlin. Accessed January 21, 2021.
http://dx.doi.org/10.17169/refubium-27486.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wittner, Moritz Stefan. “Anti-cariogenic Properties of Different Probiotic Bacteria in vitro.” 2020. Web. 21 Jan 2021.
Vancouver:
Wittner MS. Anti-cariogenic Properties of Different Probiotic Bacteria in vitro. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Jan 21].
Available from: http://dx.doi.org/10.17169/refubium-27486.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wittner MS. Anti-cariogenic Properties of Different Probiotic Bacteria in vitro. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27486
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
10.
Stanisavljević, Nemanja S., 1986-.
Karakterizacija i in vitro biološke aktivnosti fenolnih
jedinjenja graška (Pisum sativum L).
Degree: Biološki fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:10882/bdef:Content/get
► BIOLOGIJA - MOLEKULARNA BIOLOGIJA / BIOLOGY - MOLECULAR BIOLOGY
Porodica mahunarki (Fabaceae) obiluje ekonomski i poljoprivredno značajnim vrstama među kojima se po zastupljenosti gajenja u…
(more)
▼ BIOLOGIJA - MOLEKULARNA BIOLOGIJA / BIOLOGY -
MOLECULAR BIOLOGY
Porodica mahunarki (Fabaceae) obiluje ekonomski i
poljoprivredno značajnim vrstama među kojima se po zastupljenosti
gajenja u Srbiji izdvaja grašak (Pisum sativum L.) sa udelom od
4,5% u ukupnoj povrtarskoj proizvodnji. Semenjače graška, posebno
genotipova sa izrazito pigmentisanim tamnim semenima predstavljaju
značajan izvor fenolnih jedinjenja čiji su sastav i biološke
aktivnosti nedovoljno ispitani, te stoga ni potencijal ovog
materijala nije iskorišćen na odgovarajući način. Semenjače graška
čine i do 10% suve mase semena i uglavnom predstavljaju otpadni
materijal koji nastaje u procesu industrijske izolacije proteina
semena koji se koriste u humanoj i animalnoj ishrani. U ovom radu
je ispitivan sadržaj fenolnih jedinjenja prisutnih u semenjačama
deset evropskih genotipova graška kao i njihove biološke
aktivnosti. Pored toga je ispitivan i uticaj različitih rastvarača,
termičke obrade i in vitro digestije semena graška na prinos
fenolnih jedinjenja i antioksidativne aktivnosti dobijenih
ekstrakata. Dobijeni rezultati su pokazali da termički tretman i in
vitro digestija dovode do statistički značajnog povećanja sadržaja
fenolnih jedinjenja, kako u vodenim ekstraktima semena graška tako
i u ekstraktima pripremljenim pomoću organskog rastvarača. Utvrđeno
je da peptidi koji nastaju u toku in vitro digestije proteina
semena graška mogu značajno interferirati sa testovima za
određivanje antioksidativne aktivnosti, pogotovo u vodenim
rastvorima, pošto i sami poseduju detektabilne antioksidativne
aktivnosti. Pokazano je da kombinacija rastvarača koja sadrži 70%
acetona, 29% destilovane vode i 1% sirćetne kiseline najpodesnije
sredstvo za ekstrakciju ukupnih fenola iz semenjače graška, koje
daje najveći prinos kako kod genotipova sa bledom, tako i kod
genotipova sa izrazito tamno pigmentisanom semenjačom. Sadržaj
ukupnih fenola u ekstraktima dobijenim primenom pomenutog
rastvarača se kod pojedinih genotipova (Assas, MBK 168 и Aslaug)
kretao u opsegu 41,8-46,6 mg GAE/ g s.m., što su najviše do sada
zabeležene vrednosti kod graška. Isti genotipovi su ujedno sadržali
najviše taninskih materija i antocijanina i pokazivali su
najizraženije sposobnosti uklanjanja DPPH radikala i redukcije Fe3+
jona. Analizom ekstrakata semenjača pomoću UHPLC–LTQ OrbiTrap MS
tehnike identifikovano je 25 najzastupljenijih fenolnih jedinjenja
i tom prilokom je detektovano 9 jedinjenja čije prisustvo do sad
nije bilo zabeleženo u semanjačama graška...
Advisors/Committee Members: Radović, Svetlana, 1958-.
Subjects/Keywords: peas; seed coat; thermal treatment; in vitro digestion;
phenolic compounds; antioxidant activity; anti-tumor
activity.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Stanisavljević, Nemanja S., 1. (2016). Karakterizacija i in vitro biološke aktivnosti fenolnih
jedinjenja graška (Pisum sativum L). (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:10882/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stanisavljević, Nemanja S., 1986-. “Karakterizacija i in vitro biološke aktivnosti fenolnih
jedinjenja graška (Pisum sativum L).” 2016. Thesis, Univerzitet u Beogradu. Accessed January 21, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:10882/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stanisavljević, Nemanja S., 1986-. “Karakterizacija i in vitro biološke aktivnosti fenolnih
jedinjenja graška (Pisum sativum L).” 2016. Web. 21 Jan 2021.
Vancouver:
Stanisavljević, Nemanja S. 1. Karakterizacija i in vitro biološke aktivnosti fenolnih
jedinjenja graška (Pisum sativum L). [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 21].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10882/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stanisavljević, Nemanja S. 1. Karakterizacija i in vitro biološke aktivnosti fenolnih
jedinjenja graška (Pisum sativum L). [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10882/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
11.
Lemoine, Clément.
Culture in vitro de plantes halophiles du littoral breton et orientation de leur métabolisme vers la production de principes actifs pour la nutrition et la cosmétique : In vitro culture of halophytes from Brittany coast and metabolic engineering towards bioproduction of active extracts for food and cosmetic industries.
Degree: Docteur es, Environnement, 2018, Brest
URL: http://www.theses.fr/2018BRES0113
► Les plantes halophiles sont des plantes résistantes au stress salin, qui subissent une grande variété de stress dans leur environnement naturel. Ces conditions les ont…
(more)
▼ Les plantes halophiles sont des plantes résistantes au stress salin, qui subissent une grande variété de stress dans leur environnement naturel. Ces conditions les ont menées à synthétiser des molécules de défense, qui peuvent présenter des activités biologiques intéressantes de par leur structure et diversité. Dans le cadre d’une collaboration avec la PME Salipouss, trois espèces ont été choisies sur la base de tests antioxydants préliminaires, avec pour objectif d’optimiser (i) la multiplication de plants in vitro pour des cultures industrielles en serre et (ii) d’améliorer le niveau d’activité de leurs extraits. La diversité des composés potentiellement actifs présents dans ces extraits est ensuite analysée par fractionnement bioguidé, afin d’isoler des molécules valorisables. Ce fractionnement est appuyé par des analyses de composés par RMN, permettant d’obtenir des informations sur la structure des composés bio-actifs. Les résultats obtenus montrent le fort potentiel de valorisation de ces trois espèces dans l’industrie, et plus particulièrement dans la nutrition et la cosmétique.
Halophytes are salt tolerant or salt-resistant plants which undergo high stress in their natural habitat. As a consequence of environmental stresses, they produce a number of active defense molecules which display interesting biological activities because of their diverse actions or structures. For the present study, three halophytic species were selected from preliminary antioxidant screening. In collaboration with Salipouss SME, objectives of the work were (i) to optimize in vitro halophyte multiplication in order to produce biomass under greenhouse and (ii) to elicit particular metabolic pathways in order to improve extract activities. To attempt to isolate molecules with potentially valuable activities, the variety of compounds from these extracts is reduced by successive fractionations. In addition, NMR analyzes allow to obtain indications on the nature and on the structure of the active compounds. First results highlight the strong activities of the selected halophytes, making them promising candidates for industrial uses, especially in nutrition and cosmetics.
Advisors/Committee Members: Magné, Christian (thesis director), Charles, Gilbert (thesis director).
Subjects/Keywords: Halophytes; Culture in vitro; Activités biologiques; Orientation métabolique; RMN; Halophytes; In vitro culture; Biological activity; Metabolic engineering; NMR
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APA (6th Edition):
Lemoine, C. (2018). Culture in vitro de plantes halophiles du littoral breton et orientation de leur métabolisme vers la production de principes actifs pour la nutrition et la cosmétique : In vitro culture of halophytes from Brittany coast and metabolic engineering towards bioproduction of active extracts for food and cosmetic industries. (Doctoral Dissertation). Brest. Retrieved from http://www.theses.fr/2018BRES0113
Chicago Manual of Style (16th Edition):
Lemoine, Clément. “Culture in vitro de plantes halophiles du littoral breton et orientation de leur métabolisme vers la production de principes actifs pour la nutrition et la cosmétique : In vitro culture of halophytes from Brittany coast and metabolic engineering towards bioproduction of active extracts for food and cosmetic industries.” 2018. Doctoral Dissertation, Brest. Accessed January 21, 2021.
http://www.theses.fr/2018BRES0113.
MLA Handbook (7th Edition):
Lemoine, Clément. “Culture in vitro de plantes halophiles du littoral breton et orientation de leur métabolisme vers la production de principes actifs pour la nutrition et la cosmétique : In vitro culture of halophytes from Brittany coast and metabolic engineering towards bioproduction of active extracts for food and cosmetic industries.” 2018. Web. 21 Jan 2021.
Vancouver:
Lemoine C. Culture in vitro de plantes halophiles du littoral breton et orientation de leur métabolisme vers la production de principes actifs pour la nutrition et la cosmétique : In vitro culture of halophytes from Brittany coast and metabolic engineering towards bioproduction of active extracts for food and cosmetic industries. [Internet] [Doctoral dissertation]. Brest; 2018. [cited 2021 Jan 21].
Available from: http://www.theses.fr/2018BRES0113.
Council of Science Editors:
Lemoine C. Culture in vitro de plantes halophiles du littoral breton et orientation de leur métabolisme vers la production de principes actifs pour la nutrition et la cosmétique : In vitro culture of halophytes from Brittany coast and metabolic engineering towards bioproduction of active extracts for food and cosmetic industries. [Doctoral Dissertation]. Brest; 2018. Available from: http://www.theses.fr/2018BRES0113
Universidade do Estado do Rio de Janeiro
12.
Graziela da Silva Mello.
Avaliação do potencial antimalárico de Norantea brasiliensis Choisy (Marcgraviaceae) cultivada in vitro e in vivo
.
Degree: Master, 2012, Universidade do Estado do Rio de Janeiro
URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8936
;
► A malária é uma doença infecciosa causada por protozoários do gênero Plasmodium, transmitidos ao homem, principalmente, através da picada do mosquito infectado. O tratamento é…
(more)
▼ A malária é uma doença infecciosa causada por protozoários do gênero Plasmodium, transmitidos ao homem, principalmente, através da picada do mosquito infectado. O
tratamento é realizado por meio do uso de drogas, como a cloroquina, uma vez que não há vacina eficiente contra a doença. Porém, a resistência dos parasitos aos medicamentos tem levado à busca por novas substâncias com atividade antimalárica, inclusive de origem vegetal.
Nesse contexto, o presente trabalho teve por objetivo avaliar a atividade antimalárica de extratos metanólicos de Norantea brasiliensis cultivada sob condições in vivo e in vitro,
espécie nativa ocorrente em restingas, com potencial medicinal já comprovado para várias atividades. Foram desenvolvidos protocolos de calogênese e cultura de raízes da espécie visando à definição de um sistema de produção de metabólitos. Para a cultura in vitro, explantes foram inoculados em meio líquido e sólido contendo diferentes fitorreguladores e concentrações. A partir da cultura de tecidos, foram testados extratos do material produzido
biotecnologicamente para comparação com o material botânico cultivado no campo. Os testes sobre o potencial antimalárico foram realizados in vivo, utilizando-se camundongos infectados pelo Plasmodium berghei ANKA, e in vitro utilizando o Plasmodium falciparum. Em seguida
foram administrados a cloroquina e os extratos vegetais. A parasitemia foi observada seguindo os protocolos já estabelecidos pelo Laboratório de Imunofarmacologia do Instituto Oswaldo Cruz (IOC). Resultados mostraram que explantes foliares e caulinares de plantas germinadas in vitro, inoculados em meio sólido B5 suplementado com 2,0 mg.mL-1 de ANA, são as melhores fontes para a produção de raízes, apresentando maiores valores de peso fresco
e peso seco, mostrando-se um sistema promissor para a produção in vitro de metabólitos da espécie. A avaliação da atividade antimalárica in vivo revelou seu potencial a partir de extrato de raízes de planta cultivada in vivo, na concentração de 50 mg/kg apresentando redução
significativa da parasitemia quando comparada com o controle não tratado. Paralelamente, nos testes in vitro a concentração de 100 μg/kg do extrato de raízes de planta cultivada in vivo apresentou diferença significativa quando comparada com as outras concentrações testadas e
o controle negativo. Além disso, há uma tendência de aumento do efeito inibitório conforme o aumento da concentração do extrato. Os resultados indicam o potencial de atividade antimalárica em raízes de N. brasiliensis, sendo este estudo o primeiro realizado para a espécie
Malaria is an infectious disease caused by protozoa of the genus Plasmodium,
transmitted to humans primarily through the bite of an infected mosquito. The treatment is
accomplished through the use of drugs such as chloroquine, since there is no effective vaccine
against the disease. However, the resistance of parasites to drugs has led to the search for new
antimalarial substances, including vegetable. In this context, this study aimed to evaluate the…
Advisors/Committee Members: Andrea Furtado Macedo, Claudia Simões Gurgel, Silvia Amaral Gonçalves da Silva, Valber da Silva Frutuoso, Norma Albarello.
Subjects/Keywords: BOTANICA; Plant medicinal; Atividade antimalárica; Cultura in vitro de raízes; Marcgraviacea - Cultivo; Antimaláricos; Malária; Fertilização in vitro; N. brasiliensis; Planta medicinal; N.brasiliensis; In vitro roots culture; Antimalarial activity
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APA (6th Edition):
Mello, G. d. S. (2012). Avaliação do potencial antimalárico de Norantea brasiliensis Choisy (Marcgraviaceae) cultivada in vitro e in vivo
. (Masters Thesis). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8936 ;
Chicago Manual of Style (16th Edition):
Mello, Graziela da Silva. “Avaliação do potencial antimalárico de Norantea brasiliensis Choisy (Marcgraviaceae) cultivada in vitro e in vivo
.” 2012. Masters Thesis, Universidade do Estado do Rio de Janeiro. Accessed January 21, 2021.
http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8936 ;.
MLA Handbook (7th Edition):
Mello, Graziela da Silva. “Avaliação do potencial antimalárico de Norantea brasiliensis Choisy (Marcgraviaceae) cultivada in vitro e in vivo
.” 2012. Web. 21 Jan 2021.
Vancouver:
Mello GdS. Avaliação do potencial antimalárico de Norantea brasiliensis Choisy (Marcgraviaceae) cultivada in vitro e in vivo
. [Internet] [Masters thesis]. Universidade do Estado do Rio de Janeiro; 2012. [cited 2021 Jan 21].
Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8936 ;.
Council of Science Editors:
Mello GdS. Avaliação do potencial antimalárico de Norantea brasiliensis Choisy (Marcgraviaceae) cultivada in vitro e in vivo
. [Masters Thesis]. Universidade do Estado do Rio de Janeiro; 2012. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8936 ;
Universidade do Estado do Rio de Janeiro
13.
Adriane de Lacerda Nery.
Estudo in vitro de derivados sintéticos da Isoniazida e da Pirazinamida sobre Leishmania (Viannia) braziliensis.
Degree: Master, 2015, Universidade do Estado do Rio de Janeiro
URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9440
;
► As leishmanioses são um grupo de doenças causadas por protozoários do gênero Leishmania spp que afetam 98 países. No Brasil, no ano de 2013, foram…
(more)
▼ As leishmanioses são um grupo de doenças causadas por protozoários do gênero Leishmania spp que afetam 98 países. No Brasil, no ano de 2013, foram relatados 3.253 casos de leishmaniose visceral e 18.226 casos de Leishmaniose Tegumentar Americana. O tratamento de primeira escolha continua sendo realizado com antimoniais pentavalentes, e em casos de insucessos os fármacos de segunda escolha são a pentamidina e a anfotericina B. Tais medicamentos causam intensos efeitos adversos e ultimamente têm surgido cepas resistentes aos mesmos. Em áreas endêmicas têm sido cada vez mais comum o surgimento da co-infecção Leishmania com Mycobacterium tuberculosis. O tratamento para a tuberculose com pirazinamida (PZA) e isoniazida (INZ), controla a leishmaniose. Esses dados sugerem atividade anti-leishmania da PZA e da INZ. O objetivo deste trabalho foi avaliar a atividade in vitro da INZ e da PZA e seus compostos derivados (série G e série R, respectivamente) sobre Leishmania (Viannia) braziliensis. As moléculas foram testadas em monocamadas de macrófagos peritoneais de camunongos infectados com L. (V) braziliensis durante 48h. Todas as moléculas testadas inibiram o índice de infecção de forma dose dependente em comparação aos controles. As moléculas da série R foram mais ativas do que a PZA, porém o resultado foi significativo somente para a R02 (p < 0,005). Apenas a molécula R05 (76,64M) foi relativamente tóxica para macrófagos. Os compostos mais ativos foram R02, G01 e G02, cujos índices de seletividade foram 14,31, 19 e 30, respectivamente. A dosagem de nitrito foi feita em sobrenadantes de monocamadas de macrófagos peritoniais infectados e tratados com as substâncias nas concentrações 10 e 100M. A G01 e a G02 estimularam a produção de NO2 nas duas concentrações, entretanto o resultado foi estatisticamente significativo para a G02 em 100M (p < 0,0001), a G05 só estimulou óxido nítrico na maior concentração. Todos os compostos da série R estimularam NO2, contudo, o resultado foi estatisticamente significativo para a R03 e R05 a 100M (p < 0,001). Adicionalmente, foi realizado uma análise preditiva in sílico de parâmetros farmacocinéticos das moléculas mais ativas in vitro, utilizando o software admetSAR. Os dados obtidos mostraram que de forma semelhante às suas moléculas originais a G01, G02 e R02 apresentaram alta capacidade de serem absorvidas pelo trato gastrointestinal, baixo potencial hepatotoxico e carcinogênico. Juntos, esses dados demonstram que essas moléculas são seletivamente tóxicas para o parasito com potencial para serem testadas pela via oral em estudos em modelo experimental de infecção.
Leishmaniasis are a group of diseases caused by protozoan of genus Leishmania spp affecting of 98 countries. In Brazil, in the year 2013 were 3.253 reported cases of visceral leishmaniasis and 18.226 cases of cutaneous leishmaniasis. The first choice of treatment is still performed with pentavalent antimonials and in cases of failures drugs of second line of treatment are pentamidine and anfotericin B. These drugs cause…
Advisors/Committee Members: Silvia Amaral Gonçalves da Silva, Patrícia Maria Lourenço Dutra, Wallace Pacienza Lima, Wânia Ferraz Pereira Manfro.
Subjects/Keywords: Leishmania (Viannia) braziliensis; Isoniazida; Pirazinamida; Atividade in vitro; Leishmania (Viannia) braziliensis; Isoniazid and Pyrazinamide; In vitro activity; PARASITOLOGIA; Leishmania braziliensis - Teses; Isoniazida - Teses; Pirazinamida; Técnicas in vitro
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APA ·
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APA (6th Edition):
Nery, A. d. L. (2015). Estudo in vitro de derivados sintéticos da Isoniazida e da Pirazinamida sobre Leishmania (Viannia) braziliensis. (Masters Thesis). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9440 ;
Chicago Manual of Style (16th Edition):
Nery, Adriane de Lacerda. “Estudo in vitro de derivados sintéticos da Isoniazida e da Pirazinamida sobre Leishmania (Viannia) braziliensis.” 2015. Masters Thesis, Universidade do Estado do Rio de Janeiro. Accessed January 21, 2021.
http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9440 ;.
MLA Handbook (7th Edition):
Nery, Adriane de Lacerda. “Estudo in vitro de derivados sintéticos da Isoniazida e da Pirazinamida sobre Leishmania (Viannia) braziliensis.” 2015. Web. 21 Jan 2021.
Vancouver:
Nery AdL. Estudo in vitro de derivados sintéticos da Isoniazida e da Pirazinamida sobre Leishmania (Viannia) braziliensis. [Internet] [Masters thesis]. Universidade do Estado do Rio de Janeiro; 2015. [cited 2021 Jan 21].
Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9440 ;.
Council of Science Editors:
Nery AdL. Estudo in vitro de derivados sintéticos da Isoniazida e da Pirazinamida sobre Leishmania (Viannia) braziliensis. [Masters Thesis]. Universidade do Estado do Rio de Janeiro; 2015. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9440 ;
14.
KOCERGINSKY, Patrícia de Oliveira.
Criptococose e determinação do efeito antifúngico in vitro e in vivo por sistema de liberação controlada com ciclopirox olamina
.
Degree: 2013, Universidade Federal de Pernambuco
URL: http://repositorio.ufpe.br/handle/123456789/18294
► A criptococose é uma infecção fúngica predominantemente oportunista cujos principais agentes etiológicos são Cryptococcus neoformans e C. gattii. O tratamento de escolha para a micose…
(more)
▼ A criptococose é uma infecção fúngica predominantemente oportunista cujos principais
agentes etiológicos são Cryptococcus neoformans e C. gattii. O tratamento de escolha para a
micose é a anfotericina B associada ou não a 5-fluorocitosina seguido de terapia de
manutenção com fluconazol. Contudo, falhas no tratamento associadas à toxicidade e ao
aparecimento de resistência aos fármacos têm sido relatadas, o que torna essencial a
descoberta de novas alternativas terapêuticas, como a ciclopirox olamina (CPO). Neste
contexto, o objetivo deste estudo foi caracterizar e avaliar a ação in
vitro e in vivo da CPO
livre e encapsulada em lipossomas frente a amostras de Cryptococcus neoformans para futura
aplicação no tratamento da criptococose sistêmica. Foram obtidas 30 amostras de
Cryptococcus neoformans provenientes de pacientes imunocomprometidos. A preparação dos
lipossomas convencionais e furtivos de CPO foi realizada pelo método da hidratação do filme
lipídico e a caracterização foi realizada avaliando os seguintes parâmetros: tamanho de
partícula, Índice de Polidispersão (PDI) e taxa de encapsulação (EE%). Para otimização dos
constituintes lipídicos, foi realizado um planejamento fatorial fracionado a 24-1 a partir da
melhor formulação obtida nos estudos de pré-formulação. A cinética de liberação in
vitro foi
conduzida para avaliar e comparar estatisticamente o perfil de liberação dos sistemas
convencional e furtivo. Adicionalmente, testes de susceptibilidade antifúngica foram
realizados de acordo com Clinical and Laboratotry Standards Institute (CLSI). Para
caracterização molecular dos isolados, PCR fingerprinting foi conduzida utilizando os primers
M13 e URA5. O estudo in vivo foi conduzido com camundongos imunossuprimidos,
infectados com Cryptococcus neoformans (106 cels/mL) e tratados com CPO lipossomal
(Lipo-CPO) (0.5 mg/Kg). As concentrações de CPO utilizadas na forma livre e encapsuladas
em lipossomas convencionais e furtivos variaram de 0,30 a 625 µg/mL. Os resultados do
planejamento fatorial mostraram que o ponto central apresentou características proeminentes
com redução do tamanho de partícula em 17,1%; melhora do PDI em 15,34% e da quantidade
de fármaco encapsulado (25%). A cinética do lipossoma furtivo apresentou uma velocidade
de liberação mais controlada quando comparada ao lipossoma convencional. Com relação ao
teste de susceptibilidade, todos os inóculos foram susceptíveis a CPO livre, com atividade
fungistática entre 0,30 e 0,61 g/mL e fungicida entre 1,22 e 4,88 g/mL. Não houve
diferença relacionada à atividade antifúngica entre as formulações lipossomais convencionais
e furtivas. A atividade fungistática dos lipossomas foi observada em concentrações variando
de 1,22 e 2,44 g/mL. A faixa das concentrações fungicidas foi de 1,22 a 9,76 g/mL. O
padrão de bandas do URA5 revelou que todos os isolados apresentam genótipo VNI,
característico de C. neoformans. Lipo-CPO apresentou eficácia antifúngica comparada à
anfotericina B após 14 dias de infecção, reduzindo a carga…
Advisors/Committee Members: MAGALHÃES, Nereide Stela Santos (advisor), NEVES, Rejane Pereira (advisor), http://lattes.cnpq.br/1063790618901917 (advisor).
Subjects/Keywords: Criptococose;
ciclopirox olamina lipossomal;
cinética de liberação in vitro;
criptococose experimental;
Cryptococosis;
Ciclopirox olamine lipossomal;
Release kinetic in vitro;
Antifungal activity in vitro;
experimental cryptococcosis
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APA ·
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MLA ·
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APA (6th Edition):
KOCERGINSKY, P. d. O. (2013). Criptococose e determinação do efeito antifúngico in vitro e in vivo por sistema de liberação controlada com ciclopirox olamina
. (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/18294
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
KOCERGINSKY, Patrícia de Oliveira. “Criptococose e determinação do efeito antifúngico in vitro e in vivo por sistema de liberação controlada com ciclopirox olamina
.” 2013. Thesis, Universidade Federal de Pernambuco. Accessed January 21, 2021.
http://repositorio.ufpe.br/handle/123456789/18294.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
KOCERGINSKY, Patrícia de Oliveira. “Criptococose e determinação do efeito antifúngico in vitro e in vivo por sistema de liberação controlada com ciclopirox olamina
.” 2013. Web. 21 Jan 2021.
Vancouver:
KOCERGINSKY PdO. Criptococose e determinação do efeito antifúngico in vitro e in vivo por sistema de liberação controlada com ciclopirox olamina
. [Internet] [Thesis]. Universidade Federal de Pernambuco; 2013. [cited 2021 Jan 21].
Available from: http://repositorio.ufpe.br/handle/123456789/18294.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
KOCERGINSKY PdO. Criptococose e determinação do efeito antifúngico in vitro e in vivo por sistema de liberação controlada com ciclopirox olamina
. [Thesis]. Universidade Federal de Pernambuco; 2013. Available from: http://repositorio.ufpe.br/handle/123456789/18294
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
15.
Devrnja, Nina M., 1979-.
In vitro propagacija i biološka aktivnost etarskog ulja i
metanolnih ekstrakata povratiča (Tanacetum vulgare L.).
Degree: Biološki fakultet, 2017, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:16899/bdef:Content/get
► Biologija - Fiziologija biljaka / Biology - Plant physiology
Rodu Tanacetum pripadaju mnoge aromatične, lekovite i ukrasne vrste bogate biološki aktivnim sekundarnim metabolitima. Tanacetum vulgare…
(more)
▼ Biologija - Fiziologija biljaka / Biology - Plant
physiology
Rodu Tanacetum pripadaju mnoge aromatične, lekovite
i ukrasne vrste bogate biološki aktivnim sekundarnim metabolitima.
Tanacetum vulgare L. (syn. Chrysanthemum vulgare L., povratič,
vratiželja, konopljika) je višegodišnja, zeljasta biljka, poreklom
iz Evrope i centralne Azije koja se obično sreće duž puteva, pruga,
pašnjaka i polja ali i priobalnih područja. U Srbiji je ova vrsta
deo sinurbane (ruderalne) flore. Predmet istraživanja ove
disertacije bila ja fitohemijska karakterizacija sastava i
bioloških aktivnosti etarskog ulja i metanolnih ekstrakata ove
vrste. In vitro kultura T. vulgare je uspešno uspostavljena iz
semena biljaka sakupljenih u prirodi. Kultura izdanaka je uspešno
održavana i multiplikovana na MS hranljivoj podlozi uz dodatak
6-benziaminopurina (BAP) a prilikom ožiljavanja BAP je zamenjen
indolsirćetnom kiselinom (IBA). In vitro kultura korenova održavana
je u tečnoj MS hranljivoj podlozi sa dodatkom IBA koja je uticala
na povećanje biomase gajenih korenova tokom subkultura. Histoločkom
analizom sekretornih struktura in vitro gajenih izdanaka utvrđeno
je da su na listovima i stablu prisutne biserijatne glandularne
trihome sa sekrecionim sadržajem u kojem su detektovani lipidi,
terpeni i alkaloidi. Hemijska analiza metanolnih ekstrakata herbe i
korena T. vulgare ukazala je na to da su ekstrakti biljaka gajenih
in vitro kvalitativno siromašniji u odnosu na metanolne ekstrakte
biljaka iz prirode ali su se ekstrakti biljaka in vitro odlikovali
višestruko većom zastupljenošću pojedinih jedinjenja, pre svih
3,5-O-dikafeoilhininskom kiselinom. Najzastupljenija jedinjenja su
fenolne kiseline iz grupe derivata cimetne kiseline i to:
neohlorogena, 3,5-O-dikafeoilhininska i dikafeoilhininska kiselina.
Pored fenolnih kiselina detektovano je i 17 flavonoida. Relativni
udeo fenolnih kiselina i flavonoida se razlikovao u zavisnosti od
dela biljke korišćenog za pripremanje matanolnog
ekstrakta...
Advisors/Committee Members: Ćalić, Dušica.
Subjects/Keywords: Tanacetum vulgare L.; in vitro culture; essential oil;
methanol extracts; antimicrobial activity; cytotoxic activity;
insecticidal activity; induction of defense mechanisms
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APA ·
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CSE |
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APA (6th Edition):
Devrnja, Nina M., 1. (2017). In vitro propagacija i biološka aktivnost etarskog ulja i
metanolnih ekstrakata povratiča (Tanacetum vulgare L.). (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:16899/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Devrnja, Nina M., 1979-. “In vitro propagacija i biološka aktivnost etarskog ulja i
metanolnih ekstrakata povratiča (Tanacetum vulgare L.).” 2017. Thesis, Univerzitet u Beogradu. Accessed January 21, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:16899/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Devrnja, Nina M., 1979-. “In vitro propagacija i biološka aktivnost etarskog ulja i
metanolnih ekstrakata povratiča (Tanacetum vulgare L.).” 2017. Web. 21 Jan 2021.
Vancouver:
Devrnja, Nina M. 1. In vitro propagacija i biološka aktivnost etarskog ulja i
metanolnih ekstrakata povratiča (Tanacetum vulgare L.). [Internet] [Thesis]. Univerzitet u Beogradu; 2017. [cited 2021 Jan 21].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:16899/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Devrnja, Nina M. 1. In vitro propagacija i biološka aktivnost etarskog ulja i
metanolnih ekstrakata povratiča (Tanacetum vulgare L.). [Thesis]. Univerzitet u Beogradu; 2017. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:16899/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
16.
Skorić, Marijana V., 1974-.
Regulacija produkcije labdanskih diterpena u kulturi in
vitro Cistus creticus L. i analiza njihove biološke
aktivnosti.
Degree: Biološki fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:11142/bdef:Content/get
► Biologija - Fiziologija biljaka / Biology - Plant physiology
Cistus creticus L. je višegodišnja, žbunasta biljka koja nastanjuje suva i topla područja Mediterana. Smolu ove…
(more)
▼ Biologija - Fiziologija biljaka / Biology - Plant
physiology
Cistus creticus L. je višegodišnja, žbunasta biljka
koja nastanjuje suva i topla područja Mediterana. Smolu ove vrste
lokalno stanovništvo Krita sakuplja od davnina zbog izraženih
aromatičnih i lekovitih svojstava. Dominantna biološki aktivna
komponenta smole i izdanaka ove biljke su labdanski diterpeni, dok
je koren biljke još uvek nepoznatog sastava. U cilju iznalaženja
optimalnih uslova za produkciju labdanskih diterpena predmet
istraživanja ove disertacije bio je ispitivanje regulacije
produkcije labdanskih diterpena u biljkama C. creticus gajenim in
vitro pod uticajem različitih abiotičkih faktora i hranljivih
podloga sa varirajućim sadržajem različitih šećera. Uspešno je
uspostavljena in vitro kultura biljaka C. creticus na MS medijumu
bez fitohormona, pri čemu parametri rastenja i razvića eksplantata
nisu pokazali značajne razlike između ispitivanih klonova.
Optimalne koncentracije ugljenih hidrata za rastenje i razviće
biljaka C. creticus u uslovima in vitro su od 0,03 M do 0,1 M
saharoze i glukoze. Histohemijskim analizama listova izdanaka
gajenih in vitro je utvrđeno da kapitatne glandularne trihome tipa
II C. creticus predstavljaju mesto akumulacije labdanskih diterpena
i fenolnih jedinjenja. Genetička transformacija C. creticus je
izvršena pomoću Agrobacterium rhizogenes A4M70GUS, pri čemu je
uspostavljena kultura transformisanih korenova. Fitohemijska
karakterizacija biljaka C. creticus gajenih in vitro, omogućila je
identifikaciju i kvantifikaciju glavnih grupa sekundarnih
metabolita (terpena i fenolnih jedinjenja) koji se u ovim uslovima
produkuju. Labdanski diterpeni su dominantna jedinjenja u izdancima
biljaka C. creticus gajenim in vitro, a među njima su
najzastupljeniji labd-7,13-dien-15-ol i labd-7,13-dien-15-ol
acetat. Od isparljivih sekundarnih metabolita najzastupljeniji su
monoterpeni i seskviterpeni. U dihlormetanskim ekstraktima eksudata
kapitatnih trihoma tipa II najzastupljenije jedinjenje je
labd-7,13-dien-15-ol acetat. U korenovima C. creticus potvrđeno je
prisustvo fenolnih jedinjenja i masnih kiselina, dok labdanski
diterpeni nisu detektovani. Analiza sadržaja fenolnih jedinjenja
ukazala je da su u etanolnim ekstraktima izadanaka i korenova
prisutna fenolna jedinjenja kao što su hininska kiselina,
galokatehin i katehin...
Advisors/Committee Members: Todorović, Slađana.
Subjects/Keywords: Cistus creticus L.; labdane diterpenes; in vitro
culture; phenolic compounds; MVA and MEP biosynthetic pathway;
antioxidant activity; antimicrobial activity; cytotoxic
activity
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Skorić, Marijana V., 1. (2016). Regulacija produkcije labdanskih diterpena u kulturi in
vitro Cistus creticus L. i analiza njihove biološke
aktivnosti. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11142/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Skorić, Marijana V., 1974-. “Regulacija produkcije labdanskih diterpena u kulturi in
vitro Cistus creticus L. i analiza njihove biološke
aktivnosti.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 21, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:11142/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Skorić, Marijana V., 1974-. “Regulacija produkcije labdanskih diterpena u kulturi in
vitro Cistus creticus L. i analiza njihove biološke
aktivnosti.” 2016. Web. 21 Jan 2021.
Vancouver:
Skorić, Marijana V. 1. Regulacija produkcije labdanskih diterpena u kulturi in
vitro Cistus creticus L. i analiza njihove biološke
aktivnosti. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 21].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11142/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Skorić, Marijana V. 1. Regulacija produkcije labdanskih diterpena u kulturi in
vitro Cistus creticus L. i analiza njihove biološke
aktivnosti. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11142/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Norte
17.
Dantas, Gracielle Rodrigues.
Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
.
Degree: 2012, Universidade do Rio Grande do Norte
URL: http://repositorio.ufrn.br/handle/123456789/13079
► Malaria is a major parasitic disease worldwide, accounting for about 500 million cases and causing 2 million to 3 million deaths annually. Four species are…
(more)
▼ Malaria is a major parasitic disease worldwide, accounting for about 500 million cases
and causing 2 million to 3 million deaths annually. Four species are responsible for
transmitting this disease to humans: Plasmodium falciparum, Plasmodium vivax,
Plasmodium malariae and Plasmodium ovale. The parasite resistance to antimalarial
drugs and the usual limitations of the vector control implications are contributing to the
spread of the disease. The most of significant advances in the search for new
antimalarial drugs is based on natural components, the main ones being currently used
antimalarial drugs derived from plants. Research on natural products of marine origin
(particularly algae) show that some species possess
antiplasmodial activity. Knowing
that the coast of Rio Grande do Norte is home to several species of algae, the present
study was to evaluate, for the first time, the antimalarial
activity of ethanolic extracts of
seaweed Spatoglossum schroederi, Gracilaria birdiae and Udotea flabellum against
Plasmodium falciparum 3D7 strain tests and in
vitro using the murine model
(Plasmodium berghei) for evaluation in vivo. These species were ground, macerated
with ethanol for 24 hours and the extracts concentrated in rotaevaporador (45 ° C ± 5 °
C). For in
vitro tests, the extracts were diluted and tested at concentrations between 100
and 1.56 μg/ml (seven concentrations in triplicate), in order to obtain IC50 of each
extract. The cytotoxicity tests with macrophages and BGM were performed using the
MTT colorimetric assay. BGM macrophages and cells were distributed in 96 wells per
plate (1x 105 to macrophages and 1x104 cells per well for BGM) and incubated for 24h
at 37 ° C. The ethanol extracts were diluted and tested at concentrations of 100 to 1,56
μg/ml (seven concentrations in triplicate). After periods of 24 hours of incubation with
the extracts, 100 μg of MTT was added to each well, and 3 hours elapsed, the
supernatant was removed and added 200 μl of DMSO in each well. The absorbance of
each well was obtained by reading on a spectrophotometer at 570 nm filter. To evaluate
the acute toxicity in vivo, Swiss mice received a single dose (oral) 2000 mg/kg/animal of
each extract tested. The parameters of acute toxicity were observed for 8 days. For in
vivo tests, Swiss mice were inoculated with 1x105 erythrocytes infected with P. berghei.
The treatment was given first to fourth day after infection with 0.2 ml of the extracts in
doses of 1000 and 500 mg//g animal. The negative control group received 0.2 ml of 2%
Tween-20, whereas the positive control group received sub-dose of chloroquine (5
mg/kg/animal). The assessment of antimalarial
activity was done by suppressing
suppressing the parasitemia at 5 and 7 days after infection. The growth inhibition of
parasites was determined relative to negative control (% inhibition = parasitaemia in
control - parasitemia in sample / parasitemia control x 100), the mortality of animals
was monitored daily for 30 days The results showed that algae Spatoglossum…
Advisors/Committee Members: Andrade Neto, Valter Ferreira de (advisor), CPF:59626305487 (advisor), http://lattes.cnpq.br/4863082845974813 (advisor), Rocha, Hugo Alexandre de Oliveira (advisor), CPF:76111830449 (advisor), http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799567J8&dataRevisao=null (advisor).
Subjects/Keywords: Malária;
Algas marinhas;
Extratos brutos;
Atividade antiplasmódica;
Malaria;
Seaweeds;
Crude extracts;
Antiplasmodial activity
Record Details
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Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dantas, G. R. (2012). Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
. (Thesis). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/13079
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dantas, Gracielle Rodrigues. “Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
.” 2012. Thesis, Universidade do Rio Grande do Norte. Accessed January 21, 2021.
http://repositorio.ufrn.br/handle/123456789/13079.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dantas, Gracielle Rodrigues. “Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
.” 2012. Web. 21 Jan 2021.
Vancouver:
Dantas GR. Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
. [Internet] [Thesis]. Universidade do Rio Grande do Norte; 2012. [cited 2021 Jan 21].
Available from: http://repositorio.ufrn.br/handle/123456789/13079.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dantas GR. Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
. [Thesis]. Universidade do Rio Grande do Norte; 2012. Available from: http://repositorio.ufrn.br/handle/123456789/13079
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Norte
18.
Dantas, Gracielle Rodrigues.
Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
.
Degree: 2012, Universidade do Rio Grande do Norte
URL: http://repositorio.ufrn.br/handle/123456789/13079
► Malaria is a major parasitic disease worldwide, accounting for about 500 million cases and causing 2 million to 3 million deaths annually. Four species are…
(more)
▼ Malaria is a major parasitic disease worldwide, accounting for about 500 million cases
and causing 2 million to 3 million deaths annually. Four species are responsible for
transmitting this disease to humans: Plasmodium falciparum, Plasmodium vivax,
Plasmodium malariae and Plasmodium ovale. The parasite resistance to antimalarial
drugs and the usual limitations of the vector control implications are contributing to the
spread of the disease. The most of significant advances in the search for new
antimalarial drugs is based on natural components, the main ones being currently used
antimalarial drugs derived from plants. Research on natural products of marine origin
(particularly algae) show that some species possess
antiplasmodial activity. Knowing
that the coast of Rio Grande do Norte is home to several species of algae, the present
study was to evaluate, for the first time, the antimalarial
activity of ethanolic extracts of
seaweed Spatoglossum schroederi, Gracilaria birdiae and Udotea flabellum against
Plasmodium falciparum 3D7 strain tests and in
vitro using the murine model
(Plasmodium berghei) for evaluation in vivo. These species were ground, macerated
with ethanol for 24 hours and the extracts concentrated in rotaevaporador (45 ° C ± 5 °
C). For in
vitro tests, the extracts were diluted and tested at concentrations between 100
and 1.56 μg/ml (seven concentrations in triplicate), in order to obtain IC50 of each
extract. The cytotoxicity tests with macrophages and BGM were performed using the
MTT colorimetric assay. BGM macrophages and cells were distributed in 96 wells per
plate (1x 105 to macrophages and 1x104 cells per well for BGM) and incubated for 24h
at 37 ° C. The ethanol extracts were diluted and tested at concentrations of 100 to 1,56
μg/ml (seven concentrations in triplicate). After periods of 24 hours of incubation with
the extracts, 100 μg of MTT was added to each well, and 3 hours elapsed, the
supernatant was removed and added 200 μl of DMSO in each well. The absorbance of
each well was obtained by reading on a spectrophotometer at 570 nm filter. To evaluate
the acute toxicity in vivo, Swiss mice received a single dose (oral) 2000 mg/kg/animal of
each extract tested. The parameters of acute toxicity were observed for 8 days. For in
vivo tests, Swiss mice were inoculated with 1x105 erythrocytes infected with P. berghei.
The treatment was given first to fourth day after infection with 0.2 ml of the extracts in
doses of 1000 and 500 mg//g animal. The negative control group received 0.2 ml of 2%
Tween-20, whereas the positive control group received sub-dose of chloroquine (5
mg/kg/animal). The assessment of antimalarial
activity was done by suppressing
suppressing the parasitemia at 5 and 7 days after infection. The growth inhibition of
parasites was determined relative to negative control (% inhibition = parasitaemia in
control - parasitemia in sample / parasitemia control x 100), the mortality of animals
was monitored daily for 30 days The results showed that algae…
Advisors/Committee Members: Andrade Neto, Valter Ferreira de (advisor), CPF:59626305487 (advisor), http://lattes.cnpq.br/4863082845974813 (advisor), Rocha, Hugo Alexandre de Oliveira (advisor), CPF:76111830449 (advisor), http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799567J8&dataRevisao=null (advisor).
Subjects/Keywords: Malária;
Algas marinhas;
Extratos brutos;
Atividade antiplasmódica;
Malaria;
Seaweeds;
Crude extracts;
Antiplasmodial activity
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dantas, G. R. (2012). Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
. (Masters Thesis). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/13079
Chicago Manual of Style (16th Edition):
Dantas, Gracielle Rodrigues. “Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
.” 2012. Masters Thesis, Universidade do Rio Grande do Norte. Accessed January 21, 2021.
http://repositorio.ufrn.br/handle/123456789/13079.
MLA Handbook (7th Edition):
Dantas, Gracielle Rodrigues. “Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
.” 2012. Web. 21 Jan 2021.
Vancouver:
Dantas GR. Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
. [Internet] [Masters thesis]. Universidade do Rio Grande do Norte; 2012. [cited 2021 Jan 21].
Available from: http://repositorio.ufrn.br/handle/123456789/13079.
Council of Science Editors:
Dantas GR. Avaliação da atividade antimalárica de extratos obtidos de algas marinhas no litoral do Rio Grande do Norte
. [Masters Thesis]. Universidade do Rio Grande do Norte; 2012. Available from: http://repositorio.ufrn.br/handle/123456789/13079
Addis Ababa University
19.
Addisu, Asfaw.
Synthesis of some novel 2-Pyrazolyl-4(3H)-quinazolinone derivatives of potential antimalarial and antileishmanial activities
.
Degree: 2012, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/6220
► In this work, some derivatives of 2-pyrazolyl-4(3H)-quinazolinones have been synthesized by cyclization and condensation reactions. The synthesized compounds were obtained in a good yield (55.9-94%).…
(more)
▼ In this work, some derivatives of 2-pyrazolyl-4(3H)-quinazolinones have been synthesized by
cyclization and condensation reactions. The synthesized compounds were obtained in a good
yield (55.9-94%). The chemical structures of the final compounds were also verified by
spectroscopic tools (IR, 1H NMR and elemental microanalyses). The in vivo anti-malarial
activity of these compounds against P.berghei infected mice was found to be moderate at oral
dose of 48.46 μmol/kg/day. This dose is equivalent to 25 mg/kg of chloroquine phosphate which
causes 100% inhibition of the parasite. Among the synthesized compounds, 2-(3-(thiophen-2-yl)-
4,5-dihydro-1H-pyrazol-5-yl)-3-phenylquinazolin-4(3H)-one, Xa, showed better
activity with
percent suppression of 65.89. The synthesized compounds were also evaluated for their in
vitro
anti-leishmanial
activity against L. donovani. Among the synthesized compounds, compound
IXe (IC50=0.0121 μg/ml) was found to possess four fold the potency of amphotericine B
(IC50=0.0460 μg/ml) while it was two hundred sixty four (264) times more potent than
miltefosine (IC50=3.1911 μg/ml). Compound Xa (IC50=0.0211 μg/ml) was twice more potent
than amphotericine B and this is the second most active compound as anti-leishmanial agent
among the synthesized compounds. The synthesized compounds exhibited better inhibitory
activity as indicated by their lower IC50 than miltefosine, except for compound XI. Compound
XI showed the least
activity which might be due to the presence of a phenyl group on the
pyrazole N1. Furthermore, in the thiophen series cyclization of α,β-unsaturated compounds to the
corresponding pyrazoline analogs led to improvement in
activity while the phenyl series showed
conflicting results.
Keywords: 2-pyrazolyl-quinazolin-4(3H)-one, in vivo anti-malarial
activity, in
vitro antileishmanial
activity and acute toxicity
Advisors/Committee Members: Adnan A. Bekhit, (Ph.D.) (advisor).
Subjects/Keywords: 2-pyrazolyl-quinazolin-4(3H)-one; in vivo anti-malarial activity; in vitro antileishmanial activity and acute toxicity.
Record Details
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Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Addisu, A. (2012). Synthesis of some novel 2-Pyrazolyl-4(3H)-quinazolinone derivatives of potential antimalarial and antileishmanial activities
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6220
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Addisu, Asfaw. “Synthesis of some novel 2-Pyrazolyl-4(3H)-quinazolinone derivatives of potential antimalarial and antileishmanial activities
.” 2012. Thesis, Addis Ababa University. Accessed January 21, 2021.
http://etd.aau.edu.et/dspace/handle/123456789/6220.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Addisu, Asfaw. “Synthesis of some novel 2-Pyrazolyl-4(3H)-quinazolinone derivatives of potential antimalarial and antileishmanial activities
.” 2012. Web. 21 Jan 2021.
Vancouver:
Addisu A. Synthesis of some novel 2-Pyrazolyl-4(3H)-quinazolinone derivatives of potential antimalarial and antileishmanial activities
. [Internet] [Thesis]. Addis Ababa University; 2012. [cited 2021 Jan 21].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/6220.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Addisu A. Synthesis of some novel 2-Pyrazolyl-4(3H)-quinazolinone derivatives of potential antimalarial and antileishmanial activities
. [Thesis]. Addis Ababa University; 2012. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6220
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Addis Ababa University
20.
Girma, Worku.
Synthesis of some novel 2-styryl-4(3H)-quinazolinone derivatives with potential antimalarial and antileishmanial activities
.
Degree: 2012, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/6227
► Malaria and leishmaniasis are the most prevalent tropical diseases caused by protozoan parasites. Half of world's population is at risk of malaria and more than…
(more)
▼ Malaria and leishmaniasis are the most prevalent tropical diseases caused by protozoan parasites. Half of world's population is at risk of malaria and more than 2 million of new cases of leishmaniasis occur annually. There are no effective vaccines available for these diseases and current treatments suffer from several limitations. Therefore, novel drugs for malaria and leishmaniasis are much-needed.
In this work, some derivatives of 2-styryl-4(3H)-quinazolinones have been synthesized using different chemical reactions like cyclization, condensation and hydrolysis. The in vivo anti-malarial
activity of these compounds on P.berghei infected mice was found to be low to moderate with an oral dose of 0.04846 mmol/kg/day. The dose employed for the antimalarial test was equivalent to 25 mg/kg of chloroquine phosphate which causes 100% inhibition of the parasite. Among the synthesized compounds,4-{(1E)-2-[3,4-dihydro-3-(methylphenyl)-4-oxoquinazolin-2-yl]vinyl}-3- methoxyphenyl acetate IVc, showed better
activity with percent suppression of 47.44. The other test compounds IVa, IVb, IVd, and Va-Vc exhibited comparable activities. On the other hand, the synthesized compounds were evaluated for their in
vitro anti-leishmanial
activity against L. donovani. Except for Compounds IVb and IVd which has IC50 value of 3.3248 and 3.3260 μg/ml respectively, the test compounds such as IVa, IVc and Va-Vc (IC50 value 0f 0.0212-2.2654 μg/ml) exhibited better potency than the reference drug miltefosine (IC50 = 3.1911μg/ml). In addition, compound IVa (IC50 = 0.0212 μg/ml) displayed better antileishmanial
activity than the reference drug amphotericine B (IC50= 0.0460μg/ml). Compounds IVb-Vc showed less
activity than the reference drug amphotericine B.
Keywords: 2-styrylquinazolin-4(3H)-one, in vivo anti-malarial
activity, in
vitro anti-leishmanial
activity and acute toxicity.
Advisors/Committee Members: ADNAN A. BEKHIT(Prof.),(PHD) (advisor).
Subjects/Keywords: 2-styrylquinazolin-4(3H)-one; in vivo anti-malarial activity; in vitro anti-leishmanial activity and acute toxicity.
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Girma, W. (2012). Synthesis of some novel 2-styryl-4(3H)-quinazolinone derivatives with potential antimalarial and antileishmanial activities
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6227
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Girma, Worku. “Synthesis of some novel 2-styryl-4(3H)-quinazolinone derivatives with potential antimalarial and antileishmanial activities
.” 2012. Thesis, Addis Ababa University. Accessed January 21, 2021.
http://etd.aau.edu.et/dspace/handle/123456789/6227.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Girma, Worku. “Synthesis of some novel 2-styryl-4(3H)-quinazolinone derivatives with potential antimalarial and antileishmanial activities
.” 2012. Web. 21 Jan 2021.
Vancouver:
Girma W. Synthesis of some novel 2-styryl-4(3H)-quinazolinone derivatives with potential antimalarial and antileishmanial activities
. [Internet] [Thesis]. Addis Ababa University; 2012. [cited 2021 Jan 21].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/6227.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Girma W. Synthesis of some novel 2-styryl-4(3H)-quinazolinone derivatives with potential antimalarial and antileishmanial activities
. [Thesis]. Addis Ababa University; 2012. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6227
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
21.
Poljarević, Jelena M. 1983-.
Kompleks Pt (II) i Pt(IV) sa novim diaminskim ligandima:
sinteza, karakterizacija i antitumorska aktivnost.
Degree: Hemijski fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:11560/bdef:Content/get
► Hemija - Opšta i neorganska hemija / Chemistry - General and Inorganic Chemistry
U ovom radu opisana je sinteza, karakterizacija i biološka aktivnost sedam organskih…
(more)
▼ Hemija - Opšta i neorganska hemija / Chemistry -
General and Inorganic Chemistry
U ovom radu opisana je sinteza, karakterizacija i
biološka aktivnost sedam organskih jedinjenja edda-tipa, šest
kompleksa platine(IV) i pet kompleksa platine(II) sa ovim organskim
molekulima kao ligandima. Sintetisana su jedinjenja:
(S,S)-etilendiamin-N,N'-di-2-amino-(3-cikloheksil)propanska
kiselina dihidrohlorid, njoj odgovarajući metil, etil, n-propil,
n-butil, izobutil i izopentil estri i njima odgovarajući kompleksi
platine(IV) i platine(II). Ligandi su sintetisani polazeći od
neesencijalne aminokiseline, S-2-amino-3-cikloheksilpropanske
kiseline hidrohlorida, iz koje je sintetisana diamindikarboksilna
kiselina i njeni estri. U reakciji
kalijum-tetrahalogenidoplatinata(II), odnosno
kalijum-heksahalogenidoplatinata(IV) i odgovarajućeg
dehidrohlorovanog liganda dobijeni su diamindihalogenidoplatina(II)
i diamintetrahalogenidoplatina(IV) kompleksi. Strukture svih
sintetisanih jedinjenja utvrđene su standardnim spektroskopskim
metodama: infracrvenom spektroskopijom, NMR spektroskopijom,
masenom spektrometrijom dok je pretpostavljena molekulska formula
potvrđena elementalnom analizom. DFT i MM proračuni u saglasnosti
su sa rezultatima ovih metoda i dali su mogućnost pretpostavke
najstabilnijih geometrijskih izomera oktaerdaskih platina(IV)
kompleksa. Biološka istraživanja zasnovana su na ispitivanju in
vitro aktivnosti sintetisanih liganada i kompleksa na nekoliko
tumorskih ćelijskih linija: C6 i U251 glioma, L929 fibrosarkoma i
B16 melanoma, ćelije humanog melanoma A375, kancera debelog creva
HCT116, ćelije humanog karcinoma pluća A549 i adenokarcinoma dojki
MCF7. Rezultati su pokazali veoma dobru antitumorsku aktivnost ovih
jedinjenja. Od svih sintetisanih liganada ističe se ligand
O,O'-dietil-(S,S)-etilendiamin-N,N'-di-2-amino-(3-
cikloheksil)propanoat dihidrohlorid, L3, koji je pokazao izuzetno
dejstvo na izabranim tumorskim ćelijskim linijama, koje je istog
reda veličine ili bolje od cisplatine. Najaktivniji među
sintetisanim kompleksima, tetrahlorido(O,O'-dietil-(S,S)-
etilendiamin-N,N'-di-2-amino-(3-cikloheksil)propanoato)platina(IV)
kompleks, K3,...
Advisors/Committee Members: Sabo, Tibor J..
Subjects/Keywords: platinum complexes; in vitro antitumor activity;
edda-type of ligands; geometrical isomers
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Poljarević, J. M. 1. (2016). Kompleks Pt (II) i Pt(IV) sa novim diaminskim ligandima:
sinteza, karakterizacija i antitumorska aktivnost. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11560/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Poljarević, Jelena M 1983-. “Kompleks Pt (II) i Pt(IV) sa novim diaminskim ligandima:
sinteza, karakterizacija i antitumorska aktivnost.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 21, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:11560/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Poljarević, Jelena M 1983-. “Kompleks Pt (II) i Pt(IV) sa novim diaminskim ligandima:
sinteza, karakterizacija i antitumorska aktivnost.” 2016. Web. 21 Jan 2021.
Vancouver:
Poljarević JM1. Kompleks Pt (II) i Pt(IV) sa novim diaminskim ligandima:
sinteza, karakterizacija i antitumorska aktivnost. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 21].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11560/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Poljarević JM1. Kompleks Pt (II) i Pt(IV) sa novim diaminskim ligandima:
sinteza, karakterizacija i antitumorska aktivnost. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11560/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manitoba
22.
Yu, Lilei.
Identification and Antioxidant Properties of Phenolic Compounds during Production of Bread from Purple Wheat Grains and Investigation of Bread Extracts after Simulated Gastrointestinal Digestion.
Degree: Food Science, 2014, University of Manitoba
URL: http://hdl.handle.net/1993/24316
► Content of free- (FPC) and bound- phenolics (BPC) significantly (p<0.05) increased during mixing, fermenting and baking. Bread crust and crumb contained the highest FPC and…
(more)
▼ Content of free- (FPC) and bound- phenolics (BPC) significantly (p<0.05) increased during mixing, fermenting and baking. Bread crust and crumb contained the highest FPC and BPC, respectively. Antioxidant activities (AOA) followed the trends of their respective phenolic contents. HPLC analysis demonstrated that different phenolic acids showed various responses to the bread-making process. Total anthocyanin content (TAC) was significantly (p<0.05) reduced through mixing and baking, but fermentation elevated the levels. Anthocyanin extract of purple wheat exerted higher AOA than those of common wheat. Digested purple wheat extracts after in-
vitro digestion demonstrated significantly (p<0.05) higher AOA than common wheat. During in-
vitro testing, extracts exhibited concentration-dependent effects, while the use of different cell lines exhibited varying levels of cellular antioxidant and pro-oxidant properties. Purple wheat demonstrated higher cytoprotectivity and cellular AOA than those of common wheat. Our findings suggest that purple wheat has the potential to act as functional food in bakery products.
Advisors/Committee Members: Beta, Trust (Food Science) (supervisor), Sapirstein, Harry (Food Science) Diehl-Jones, William (Faculty of Nursing) (examiningcommittee).
Subjects/Keywords: Purple wheat; Bread-making; Phenolic compounds; anthocyanins; antioxidant activity; in-vitro digestion; cell culture
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yu, L. (2014). Identification and Antioxidant Properties of Phenolic Compounds during Production of Bread from Purple Wheat Grains and Investigation of Bread Extracts after Simulated Gastrointestinal Digestion. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/24316
Chicago Manual of Style (16th Edition):
Yu, Lilei. “Identification and Antioxidant Properties of Phenolic Compounds during Production of Bread from Purple Wheat Grains and Investigation of Bread Extracts after Simulated Gastrointestinal Digestion.” 2014. Masters Thesis, University of Manitoba. Accessed January 21, 2021.
http://hdl.handle.net/1993/24316.
MLA Handbook (7th Edition):
Yu, Lilei. “Identification and Antioxidant Properties of Phenolic Compounds during Production of Bread from Purple Wheat Grains and Investigation of Bread Extracts after Simulated Gastrointestinal Digestion.” 2014. Web. 21 Jan 2021.
Vancouver:
Yu L. Identification and Antioxidant Properties of Phenolic Compounds during Production of Bread from Purple Wheat Grains and Investigation of Bread Extracts after Simulated Gastrointestinal Digestion. [Internet] [Masters thesis]. University of Manitoba; 2014. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/1993/24316.
Council of Science Editors:
Yu L. Identification and Antioxidant Properties of Phenolic Compounds during Production of Bread from Purple Wheat Grains and Investigation of Bread Extracts after Simulated Gastrointestinal Digestion. [Masters Thesis]. University of Manitoba; 2014. Available from: http://hdl.handle.net/1993/24316
23.
Figueiredo, Sônia Aparecida.
Avaliação in vitro e in vivo do potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis (EEEGb).
Degree: Mestrado, Medicamentos e Cosméticos, 2013, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/60/60137/tde-21062013-133538/
;
► A radiação solar ultravioleta (RUV) pode induzir efeitos à pele devidos a sua ação direta ou indireta, por meio da geração de radicais livres. Esses…
(more)
▼ A radiação solar ultravioleta (RUV) pode induzir efeitos à pele devidos a sua ação direta ou indireta, por meio da geração de radicais livres. Esses efeitos podem provocar diversas lesões na pele humana como o câncer de pele. No Brasil, segundo o Instituto Nacional do Câncer (INCA, 2012), este tipo de câncer corresponde a 25% de todos os tumores diagnosticados. Como medida profilática de proteção da pele contra os efeitos da radiação solar pode-se citar o uso de protetores solares, produtos tópicos adicionados de filtros solares UV sintéticos com propriedades de absorção e reflexão dos raios solares, e como medida preventiva é recomendado o uso de fotoquimioprotetores, produtos tópicos ou de administração oral incorporados de extratos vegetais ou substancias naturais isoladas com atividades antioxidante e/ou sequestradora de radicais livres e atividade anti-inflamatória. Os protetores solares são considerados produtos OTC, em alguns países, e por isso devem ter sua eficácia comprovada por métodos in
vitro ou in vivo padronizados. Assim, o presente trabalho teve como objetivo investigar o potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis usando métodos in
vitro e in vivo, respectivamente. O extrato foi caracterizado quimicamente por medida dos teores de flavonoides, polifenois e lipídios e funcionalmente pela determinação da atividade antioxidante e/ou sequestradora de radicais livres por diferentes métodos in
vitro. A citotoxidade e fotoestabilidade do extrato, como também, o potencial fotoprotetor do extrato e das formulações adicionadas deste em diferentes concentrações foram avaliados in
vitro por medida da viabilidade celular de cultura de células de fibroblastos (L929). A eficácia fotoprotetora e/ou fotoquimioprotetora da formulação adicionada do extrato foi testada in vivo por medida das quantidades de GSH endógeno e das interleucinas IL-1? e TNF-?, como também, pela medida da atividade da mieloperoxidase, usando os camundongos hairless, como modelo animal. Os teores de flavonoides e polifenois de 3,4 mg EQ/g e 69,84 mg EAG/g, respectivamente, foram menores àqueles de outros extratos vegetais. Os menores teores de flavonoides e polifenois refletiram na menor atividade antioxidante desse extrato que apresentou valores de IC50 de 47,47 ?g/mL e 425,06 ?g/mL para atividade antioxidante determinada pelos métodos de DPPHo e peroxidação lipídica, respectivamente. O teor de lipídio encontrado foi de 45%, isto sugere que esse extrato deve ser armazenado em condições controladas para minimizar a sua instabilidade por meio da oxidação dos lipídios por reações oxidativas. Os estudos de citotoxidade mostraram que o extrato na concentração de 25 ?g/mL diminuiu em 40% a viabilidade das células L929. A citotoxidade do extrato foi maior quando exposto à radiação UVA que à UVB, sugerindo que este extrato pode ser mais fotoinstável à radiação UVA. Nos testes de fotoproteção empregando culturas de células, o extrato na concentração de 100 mg/mL e formulação adicionada de 20%…
Advisors/Committee Members: Fonseca, Maria Jose Vieira.
Subjects/Keywords: antioxidant activity; atividade antioxidante; citotoxidade; cytotoxicity; fotoproteção; fotoquimioproteção; Garcinia brasiliensis; Garcinia brasiliensis; in vitro method; in vivo method.; método in vitro; método in vivo; photochemioprotection; photoprotection
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Figueiredo, S. A. (2013). Avaliação in vitro e in vivo do potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis (EEEGb). (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/60/60137/tde-21062013-133538/ ;
Chicago Manual of Style (16th Edition):
Figueiredo, Sônia Aparecida. “Avaliação in vitro e in vivo do potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis (EEEGb).” 2013. Masters Thesis, University of São Paulo. Accessed January 21, 2021.
http://www.teses.usp.br/teses/disponiveis/60/60137/tde-21062013-133538/ ;.
MLA Handbook (7th Edition):
Figueiredo, Sônia Aparecida. “Avaliação in vitro e in vivo do potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis (EEEGb).” 2013. Web. 21 Jan 2021.
Vancouver:
Figueiredo SA. Avaliação in vitro e in vivo do potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis (EEEGb). [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 21].
Available from: http://www.teses.usp.br/teses/disponiveis/60/60137/tde-21062013-133538/ ;.
Council of Science Editors:
Figueiredo SA. Avaliação in vitro e in vivo do potencial fotoprotetor e/ou fotoquimioprotetor do extrato etanólico do epicarpo de Garcinia brasiliensis (EEEGb). [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/60/60137/tde-21062013-133538/ ;
Univerzitet u Beogradu
24.
Tošić, Svetlana M., 1969-.
Fiziološki i biohemijski aspekti propagacije endemičnih
vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica
(Pers.) Schott in vitro.
Degree: Biološki fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:10897/bdef:Content/get
► Biologija - Fiziologija i molekularna biologija biljaka / Biology - Physiology and Molecular biology of Plants
aromatičnih biljaka koje se koriste u tradicionalnoj medicini, hortikulturi,…
(more)
▼ Biologija - Fiziologija i molekularna biologija
biljaka / Biology - Physiology and Molecular biology of
Plants
aromatičnih biljaka koje se koriste u
tradicionalnoj medicini, hortikulturi, hemijskoj, prehrambenoj i
kozmetičkoj industriji. Retke, endemične i/ili ugrožene vrste
familije Lamiaceae predstavljaju poseban izazov za proučavanje,
prevashodno u cilju očuvanja specijskog diverziteta i njihovih
populacija u prirodi, ali i zato što su izvor važnih bioaktivnih
molekula raznovrsnog dejstva (antimikrobna, antvirusna, fungicidna,
antioksidantna, citotoksična, alelohemijska, insekticidna
aktivnost, itd.) i potencijalno široke primene. Usled izrazite
heterogenosti, predstavnici roda Micromeria su bili često predmet
taksonomskih diskusija. Pripadnike sekcije Pseudomelissa gde spada
i М. pulegium, nakon molekularno genetičkih analiza hloroplastne
DNK, Brauchler (2005) premešta u rod Clinopodium. Ipak, u ovoj tezi
je primenjen tradicionalni taksonomski koncept, predložen od strane
Harley-a i sar. (2004), koji podržava homogenost roda Micromeria,
pri čemu je sect. Pseudomelissa njegova sastavna jedinica. U skladu
sa primenjenim sistemom dve istraživane vrste su klasifikovane u
idividualne sekcije roda: Micromeria sect. Micromeria (M.
croatica), odnosno Micromeria sect. Pseudomelissa (M. pulegium).
Micromeria pulegium predstavlja endemičnu vrstu Južnih Karpata u
Rumuniji, sa enklavom u istočnoj Srbiji, a Micromeria croatica
balkansku endemičnu vrstu čiji se areal vezuje pre svega za
planinski venac Dinarida. Njihove prirodne populacije imaju mali
broj jedinki i nastanjuju staništa koja su pod negativnim uticajem
antropogenih faktora. Imajući u vidu značaj roda Micromeria, kao i
činjenicu da su pomenute vrste retke i ugrožene, javila se potreba
da se one gaje zarad očuvanja i istraživanja bez pritiska na
prirodne populacije i bez remećenja prirodnog genofonda. Iz
pomenutih razloga obe vrste roda Micromeria uvedene su kulturu
biljnih tkiva in vitro. U ovoj disertaciji vršeno je ispitivanje
efekata regulatora rastenja na morfogenezu i produkciju etarskih
ulja endemičnih vrsta M. pulegium i M. croatica in
vitro...
Advisors/Committee Members: Stojičić, Dragana.
Subjects/Keywords: Micromeria pulegium; Micromeria croatica; in vitro; BA;
kinetin; auxins; trichome; essential oils; antioxidant activity;
antimicrobial activity
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tošić, Svetlana M., 1. (2016). Fiziološki i biohemijski aspekti propagacije endemičnih
vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica
(Pers.) Schott in vitro. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:10897/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tošić, Svetlana M., 1969-. “Fiziološki i biohemijski aspekti propagacije endemičnih
vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica
(Pers.) Schott in vitro.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 21, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:10897/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tošić, Svetlana M., 1969-. “Fiziološki i biohemijski aspekti propagacije endemičnih
vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica
(Pers.) Schott in vitro.” 2016. Web. 21 Jan 2021.
Vancouver:
Tošić, Svetlana M. 1. Fiziološki i biohemijski aspekti propagacije endemičnih
vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica
(Pers.) Schott in vitro. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 21].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10897/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tošić, Svetlana M. 1. Fiziološki i biohemijski aspekti propagacije endemičnih
vrsta Micromeria pulegium (Rochel) Benth. i Micromeria croatica
(Pers.) Schott in vitro. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10897/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
25.
Krania, Fotini.
Επίδραση ειδικών πρωτεολυτικών ενζύμων στην εξωσωματική παραγωγή και στα ποιοτικά χαρακτηριστικά των εμβρύων αγελάδων.
Degree: 2015, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας
URL: http://hdl.handle.net/10442/hedi/35458
► Here, we examined the effects of in vitro fertilization (IVF) and in vitro culture (IVC) media modification with the addition of tissue-type plasminogen activator (t-PA)…
(more)
▼ Here, we examined the effects of in vitro fertilization (IVF) and in vitro culture (IVC) media modification with the addition of tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA), respectively, on bovine embryo development and quality, assessed by quantification of various genes’ expression used as markers of embryo viability. Plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in all IVF and IVC spent media by a spectrophotometric method. In experiment 1, 2016 cumulus-oocyte complexes (COCs) were divided into 4 groups with modified composition of the IVF medium containing t-PA and/or its inhibitor epsilon-aminocaproic acid (control, t-PA, t-PA+ε-ACA, ε-ACA). t-PA was used at a final activity of 500 IU/ml, while inhibitor’s final concentration was 10 mM. The 2nd experiment is divided into two subgroups, where the effect of IVC medium modification with 5 IU/ml of u-PA is examined on denuded embryos cultured in semi-defined synthetic oviductal fluid (SOF) for seven days (experiment 2.a, 1631 putative zygotes) and on embryos co-cultured on cumulus cells monolayer in serum-containing SOF medium (experiment 2.b, 629 putative zygotes). Relative quantification of all mRNA trancripts was performed using real-time polymerase chain reaction (real-time PCR). To evaluate the quality of produced embryos (Day 7 blastocysts), a set of genes has been selected to be analyzed (PLAC8, AKR1B1, BIRC5 or survivin, BBC3 or PUMA, PΤGS-2 or COX-2, BCL2L1, SLC2A5 or GLUT-5, MnSOD, PLG and PLAUR); their expression is indicative of embryo quality. For Day 3 and Day 4 embryo gene analysis, another group of genes was selected to be studied (BAX, BCL2L1, PIPOX, G6PD, SLC2A5, MnSOD and KAT2B or PCAF). Under our experimental conditions, it appears that exogenously added t-PA to IVF media, suppresses cleavage and bovine blastocyst formation rates. The low IVP rates were reversed, when t-PA activity was reduced by ε-ACA addition. Apoptosis or cell cycle arrest at early stage embryos (morulae) may be favored by this modification reflecting a possible negative effect of t-PA addition on embryo quality. In the 2nd experiment, despite u-PA’s significant role in early and late embryo development, the inclusion of exogenous u-PA in the in vitro bovine embryo culture medium did not have any effect on embryo yield and/or quality under our experimental culture conditions. However, much research is needed to unveil the mechanism by which t-PA and u-PA are involved in in vitro embryo production systems. Even though the above experiments did not favor IVP results and embryo quality, those two activators are definitely involved into fertilization and embryo culture necessitating further investigation of their possible mechanisms and interactions into these processes.
Στην παρούσα διατριβή μελετάται η επίδραση της τροποποίησης των in vitro υποστρωμάτων της γονιμοποίησης (IVF) και της καλλιέργειας των εμβρύων (IVC) με την προσθήκη του ενεργοποιού του πλασμινογόνου του ιστικού τύπου…
Subjects/Keywords: In vitro γονιμοποίηση; In vitro καλλιέργεια εμβρύων; Γονιδιακή έκφραση; Ενζυμική δραστηριότητα ενεργοποιών του πλασμινογόνου και αδρανοποιών τους; Διαφορική χρώση κυττάρων; Απόπτωση; In vitro fertilization; in vitro culture of embryos; Gene expression; Enzymatic activity of plasminogen activators and/or plasminogen activators inhibitors; Differential cell staining; Apoptosis
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APA (6th Edition):
Krania, F. (2015). Επίδραση ειδικών πρωτεολυτικών ενζύμων στην εξωσωματική παραγωγή και στα ποιοτικά χαρακτηριστικά των εμβρύων αγελάδων. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/35458
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Krania, Fotini. “Επίδραση ειδικών πρωτεολυτικών ενζύμων στην εξωσωματική παραγωγή και στα ποιοτικά χαρακτηριστικά των εμβρύων αγελάδων.” 2015. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed January 21, 2021.
http://hdl.handle.net/10442/hedi/35458.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Krania, Fotini. “Επίδραση ειδικών πρωτεολυτικών ενζύμων στην εξωσωματική παραγωγή και στα ποιοτικά χαρακτηριστικά των εμβρύων αγελάδων.” 2015. Web. 21 Jan 2021.
Vancouver:
Krania F. Επίδραση ειδικών πρωτεολυτικών ενζύμων στην εξωσωματική παραγωγή και στα ποιοτικά χαρακτηριστικά των εμβρύων αγελάδων. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2015. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10442/hedi/35458.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Krania F. Επίδραση ειδικών πρωτεολυτικών ενζύμων στην εξωσωματική παραγωγή και στα ποιοτικά χαρακτηριστικά των εμβρύων αγελάδων. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2015. Available from: http://hdl.handle.net/10442/hedi/35458
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Sul
26.
Gomes, Madson Ralide Fonseca.
Estudo químico, biológico e toxicológico de drimys angustifolia Miers e drymis brasiliensis miers.
Degree: 2012, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/139449
► O gênero Drimys é o de maior área de distribuição geográfica da família (Winteraceae), que compreende sete gêneros e cerca de 120 espécies. No Brasil,…
(more)
▼ O gênero Drimys é o de maior área de distribuição geográfica da família (Winteraceae), que compreende sete gêneros e cerca de 120 espécies. No Brasil, encontra-se desde a Bahia até o Rio Grande do Sul e ocorre em duas espécies, Drimys angustifolia Miers. e Drimys brasiliensis Miers, conhecidas popularmente como “casca-de-anta”. As folhas e cascas são usadas na medicina popular como antiescorbútico, estimulante, antiespasmódica, antidiarreica, antifebril, contra hemorragia uterina, antibacteriana, no tratamento de asma, bronquite, certas afecções do trato digestivo e, algumas vezes no tratamento do câncer e inseticidas. Ambas são caracterizadas pela presença de flavonóides e sesquiterpenoides. O objetivo deste trabalho foi o de avaliar a composição química, o potencial antioxidante, antiviral, inseticida, antitumoral, antifúngico, avaliação tóxica aguda e, também, formular e caracterizar duas nanoemulsões dos óleos voláteis das duas espécies do estudo. Por meio da hidrodestilação, foi possível realizar a extração dos óleos essenciais e determinar seus rendimentos que variaram entre 0,3 e 0,5% de folhas. A identificação dos componentes foi realizada utilizando a Cromatrografia Gasosa acoplada ao espectrofotômetro de massas (CG-EM), as quais apresentaram como componentes majoritários em todas as análises, o biciclogermacreno para a espécie Drimys angustifolia e a ciclocolorenona para a espécie Drimys brasiliensis. A formulação e caracterização da nanoemulsão foi realizada dentro dos parâmetros estabelecidos (tamanho de partícula, índice de polidispersão, pH) e através da microscopia eletrônica de transmissão foi confirmado o tamanho na escala nano. No que tange a atividade inseticida, ambas as espécies mostraram-se promissoras repelindo o cupim da espécie Cryptotermes brevis com índices de repelência negativos. Para avaliação inicial do potencial larvicida de Aedes aegypti, os óleos das duas espécies não foram eficientes. No entanto, a nanoemulsão de Drimys brasiliensis levou a 69% e 89% de mortalidade, correspondendo às concentrações de 0,5 μg/mL e 0,83 μg/mL, respectivamente. Com relação a atividade antioxidante, os óleos das duas espécies não foram capazes de reduzir o radical (2,2-difenil-1-picril-hidrazil) DPPH . Já na atividade antiviral frente ao VHS-1, as duas espécies mostraram-se promissoras inibindo a replicação viral. Na atividade antitumoral nas linhagens de glioma (U-138 MG) e de bexiga (T24), somente o óleo de Drimys brasiliensis reduziu a viabilidade celular das duas linhagens pelo ensaio do MTT, com resultados similares na contagem celular. Na análise da citometria pela incorporação da Anexina-V/Iodeto de propídio caracterizou-se indução de apoptose tardia. Na atividade antifúngica, as duas espécies foram eficazes contra o fungo oportunista Acremonium, presente em diversas infecções em transplantados e imunocomprometidos. Quanto à avaliação da toxicidade aguda, as duas espécies mostraram importantes sinais de toxicidade em ratos Wistar como ptose, tremor, redução da atividade motora, exolftamia,…
Advisors/Committee Members: Limberger, Renata Pereira.
Subjects/Keywords: Drimys angustifolia; Drimys; Essential oils; Drimys brasiliensis; Biological activity; Winteraceae; In vitro toxicity; Óleos essenciais; Toxicidade; In vivo toxicity
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APA (6th Edition):
Gomes, M. R. F. (2012). Estudo químico, biológico e toxicológico de drimys angustifolia Miers e drymis brasiliensis miers. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/139449
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gomes, Madson Ralide Fonseca. “Estudo químico, biológico e toxicológico de drimys angustifolia Miers e drymis brasiliensis miers.” 2012. Thesis, Universidade do Rio Grande do Sul. Accessed January 21, 2021.
http://hdl.handle.net/10183/139449.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gomes, Madson Ralide Fonseca. “Estudo químico, biológico e toxicológico de drimys angustifolia Miers e drymis brasiliensis miers.” 2012. Web. 21 Jan 2021.
Vancouver:
Gomes MRF. Estudo químico, biológico e toxicológico de drimys angustifolia Miers e drymis brasiliensis miers. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2012. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10183/139449.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gomes MRF. Estudo químico, biológico e toxicológico de drimys angustifolia Miers e drymis brasiliensis miers. [Thesis]. Universidade do Rio Grande do Sul; 2012. Available from: http://hdl.handle.net/10183/139449
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Federal de Viçosa
27.
Cláudia Martellet Fogaça.
Tolerância aos estresses salino e térmico em cultivares de taro e mandioca tuberizadas in vitro.
Degree: 2007, Universidade Federal de Viçosa
URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=526
► A tuberização é um complexo processo de desenvolvimento que é influenciado por variáveis genéticas, ambientais e fisiológicas, sendo caracterizado por significativas mudanças anatômicas, hormonais e…
(more)
▼ A tuberização é um complexo processo de desenvolvimento que é influenciado por variáveis genéticas, ambientais e fisiológicas, sendo caracterizado por significativas mudanças anatômicas, hormonais e bioquímicas. Nesse sentido, a microtuberização in
vitro, além de sua importância pela rápida propagação vegetativa das plantas livres de doenças, manutenção e manipulação de material, facilidade de conservação e intercâmbio de germoplasma, se presta como sistema experimental para o melhor entendimento de processos associados à indução e controle da tuberização. Com o objetivo de induzir o processo de microtuberização in
vitro de cultivares de mandioca e de taro foi analisado a ação de diferentes reguladores de crescimento, BAP e ANA, doses de sacarose (3%, 6% e 8% p/v) e regime luminoso diferenciado. Foram analisadas características morfológicas como o comprimento da parte aérea (CPA), o número médio de raízes (NR), o diâmetro de rizomas (DR), o padrão de desenvolvimento dos microtubérculos, bem como características anatômicas. Com base nos resultados verificou-se que as cultivares de taro e mandioca respondem diferentemente à microtuberização in
vitro. A cultivar de taro Japonês respondeu melhor ao processo de indução de microrizomas, principalmente quando submetida ao tratamento com 22,2 M BAP e 8% (p/v) sacarose. Já em mandioca, a cultivar Parazinha respondeu melhor à indução de raízes tuberiformes, tendo o tratamento constituído de 0,4 M BAP , 1,6 M ANA e 8% (p/v) sacarose como maior indutor na formação de raiz tuberiforme, tanto em meio semisólido quanto em meio líquido. No comprimento médio da parte aérea, comprimento médio da raiz mais longa e número médio de raízes destacou-se a cultivar de mandioca Mantiqueira, porém, em meio de indução à raiz tuberiforme, foi considerada recalcitrante. Anatomicamente, a microtuberização in
vitro caracterizou-se pela presença de grãos de amido nos microrizomas e raízes tuberiformes de taro e mandioca, respectivamente. Para estudar os efeitos do estresse salino e de baixa temperatura in
vitro, cultivares de taro e mandioca tuberizadas em meio MS foram transferidas para meio com diferentes concentrações salinas (0, 50, 75 e 100 mmol L-1 de NaCl) ou submetidas a diferentes temperaturas (5, 10 e 25C), por um período de 7 dias. Foram mensurados os níveis de etileno aos 5, 20 e 40 dias de cultivo para o experimento de estresse salino, e aos 3 e 7 dias para os de estresse térmico. Também, foram realizadas análises bioquímicas de enzimas antioxidantes (POD, PPO e CAT) e isoenzimas, além da análise das características morfológicas decorrentes do estresse abiótico. A adição de NaCl ao meio de cultivo induziu o estresse oxidativo, caracterizado pelo aumento da atividade das enzimas POD e CAT, e elevada concentração de etileno em mandioca. Em meio líquido, o NaCl elevou a produção de etileno e aumento da atividade da CAT na mandioca Parazinha; verificou-se também, diferença de tolerância ao estresse térmico e salino entre as cultivares de mandioca e taro. A baixa temperatura, em mandioca,…
Advisors/Committee Members: Wagner Campos Otoni, Fernando Luiz Finger, Mário Puiatti, Maria Aparecida Nogueira Sediyama, Vicente Wagner Dias Casali.
Subjects/Keywords: Enzyme activity; MELHORAMENTO VEGETAL; Minituberização in vitro; Estresse salino e térmico; Atividade de enzima; Micro tuberization in vitro; Salt and thermal stresses
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APA ·
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CSE |
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APA (6th Edition):
Fogaça, C. M. (2007). Tolerância aos estresses salino e térmico em cultivares de taro e mandioca tuberizadas in vitro. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=526
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fogaça, Cláudia Martellet. “Tolerância aos estresses salino e térmico em cultivares de taro e mandioca tuberizadas in vitro.” 2007. Thesis, Universidade Federal de Viçosa. Accessed January 21, 2021.
http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=526.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fogaça, Cláudia Martellet. “Tolerância aos estresses salino e térmico em cultivares de taro e mandioca tuberizadas in vitro.” 2007. Web. 21 Jan 2021.
Vancouver:
Fogaça CM. Tolerância aos estresses salino e térmico em cultivares de taro e mandioca tuberizadas in vitro. [Internet] [Thesis]. Universidade Federal de Viçosa; 2007. [cited 2021 Jan 21].
Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=526.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fogaça CM. Tolerância aos estresses salino e térmico em cultivares de taro e mandioca tuberizadas in vitro. [Thesis]. Universidade Federal de Viçosa; 2007. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=526
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université de Bordeaux I
28.
Moretti, Daniela.
Exposure of neuronal networks to GSM mobile phone signals : Exposition de réseaux de neurones à des signaux de téléphonie mobile de type GSM.
Degree: Docteur es, Electronique, 2013, Université de Bordeaux I
URL: http://www.theses.fr/2013BOR14864
► Le système nerveux central est la cible la plus probable d'effets biologiques dûs à l'exposition aux radiofréquences (RF) de la téléphonie mobile. Plusieurs études sur…
(more)
▼ Le système nerveux central est la cible la plus probable d'effets biologiques dûs à l'exposition aux radiofréquences (RF) de la téléphonie mobile. Plusieurs études sur l’EEG (électroencéphalogramme) ont montré des variations dans le spectre de la bande alpha pendant et / ou après l'exposition aux radiofréquences, avec les yeux fermés ou pendant le sommeil. Dans ce contexte, l'observation de l'activité électrique spontanée des réseaux neuronaux sous exposition aux radiofréquences représente un outil efficace pour détecter de possibles effets des RF de faible niveau sur le système nerveux. Dans ce travail de thèse, nous avons développé un dispositif expérimental dédié à l'exposition dans la gamme des GHz de réseaux neuronaux et permettant simultanément l’enregistrement de l'activité électrique des neurones. Une cellule électromagnétique transversale (TEM) a été utilisée afin d'exposer les réseaux neuronaux aux signaux GSM-1800 à un niveau de DAS de 3,2 W / kg. L'enregistrement de l'activité électrique neuronale et la détection en termes de spikes et bursts sous exposition ont été réalisées à l'aide de réseaux de micro-électrodes (MEAs). Ce travail démontre la faisabilité de l’étude (culture de réseaux de neurones primaires, enregistrement de l'activité électrique et analyse des signaux obtenus sous exposition aux radiofréquences) et expose des résultats préliminaires. Dans l'expérience principale (16 cultures), il y avait une diminution réversible de 30% du taux moyen de spikes (MFR) et de bursts (BR) pendant les 3 min d’exposition aux RF. Des expériences supplémentaires sont nécessaires pour mieux caractériser cet effet, notamment en termes d'élévation de la température au niveau microscopique.
The central nervous system is the most likely target of mobile telephony radiofrequency field (RF) exposure in terms of biological effects. Several EEG (electroencephalography) studies have reported variations in the alpha-band power spectrum during and/or after RF exposure, in resting EEG and during sleep. In this context, the observation of the spontaneous electrical activity of neuronal networks under RF exposure can be an efficient tool to detect the occurrence of low-level RF effects on the nervous system. In this thesis research work we developed a dedicated experimental setup in the GHz range for the simultaneous exposure of neuronal networks and monitoring of electrical activity. A transverse electromagnetic (TEM) cell was used to expose the neuronal networks to GSM-1800 signals at a SAR level of 3.2 W/kg. Recording of the neuronal electrical activity and detection of the extracellular spikes and bursts under exposure were performed using Micro Electrode Arrays (MEAs). This work provides the proof of feasibility and preliminary results of the integrated investigation regarding exposure setup, culture of the neuronal network, recording of the electrical activity and analysis of the signals obtained under RF exposure. In the main experiment (16…
Advisors/Committee Members: Lewis, Noëlle (thesis director), Garenne, André (thesis director).
Subjects/Keywords: Étude de faisabilité; Signal GSM-1800; Réseaux de neurones; Activité électrique; Micro Electrode Arrays; In vitro; Feasibility study; GSM-1800 signal; Neuronal networks; Electrical activity; Micro Electrode Arrays; In vitro
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APA ·
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APA (6th Edition):
Moretti, D. (2013). Exposure of neuronal networks to GSM mobile phone signals : Exposition de réseaux de neurones à des signaux de téléphonie mobile de type GSM. (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2013BOR14864
Chicago Manual of Style (16th Edition):
Moretti, Daniela. “Exposure of neuronal networks to GSM mobile phone signals : Exposition de réseaux de neurones à des signaux de téléphonie mobile de type GSM.” 2013. Doctoral Dissertation, Université de Bordeaux I. Accessed January 21, 2021.
http://www.theses.fr/2013BOR14864.
MLA Handbook (7th Edition):
Moretti, Daniela. “Exposure of neuronal networks to GSM mobile phone signals : Exposition de réseaux de neurones à des signaux de téléphonie mobile de type GSM.” 2013. Web. 21 Jan 2021.
Vancouver:
Moretti D. Exposure of neuronal networks to GSM mobile phone signals : Exposition de réseaux de neurones à des signaux de téléphonie mobile de type GSM. [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2013. [cited 2021 Jan 21].
Available from: http://www.theses.fr/2013BOR14864.
Council of Science Editors:
Moretti D. Exposure of neuronal networks to GSM mobile phone signals : Exposition de réseaux de neurones à des signaux de téléphonie mobile de type GSM. [Doctoral Dissertation]. Université de Bordeaux I; 2013. Available from: http://www.theses.fr/2013BOR14864
29.
Lemoine, Manuela.
La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.
Degree: Docteur es, Sciences de la vie, 2009, Université François-Rabelais de Tours
URL: http://www.theses.fr/2009TOUR4005
► L’objectif de la thèse a été d’apporter des éléments sur la réaction acrosomique (RA) aviaire afin de mieux comprendre les processus menant à la fécondation…
(more)
▼ L’objectif de la thèse a été d’apporter des éléments sur la réaction acrosomique (RA) aviaire afin de mieux comprendre les processus menant à la fécondation et de mieux maîtriser la capacité des spermatozoïdes à être conservés. Nos résultats ont conforté l’hypothèse de l’absence de capacitation chez les oiseaux. De plus, il n’y a pas d’hyperactivation de la mobilité lors de la RA. Seul le Ca2+ s’avère être l’élément indispensable au déclenchement de la RA. L’évaluation de la RA avec des spermatozoïdes conservés à l’état liquide ou après cryoconservation a révélé une évolution différente en fonction du type de conservation. L’étude des voies de signalisation susceptibles d’être impliquées dans le déclenchement de la RA a suggéré l’activation de 3voies, PKA, PI3K et MAPK ERK. Ce travail ouvre de nombreuses perspectives scientifiques vers l’approfondissement des connaissances de la RA chez les oiseaux et sur l’utilisation qui peut en être faite pour mieux maîtriser la qualité des gamètes.
The aim of this work was to provide new information on chicken acrosome reaction (AR) for a better comprehension of the mechanisms leading to this reaction and a better control of the fertilizing potential of spermatozoa after in vitro storage. Our results showed that calcium is the factor absolutely necessary to initiate the AR and supported the hypothesis that chicken spermatozoa do not need to be capacitated. Moreover, motility hyperactivation was not found at the time of AR. Then, we showed that chicken sperm ability to undergo the AR may differ depending on the type of semen storage. Indeed, this ability was dramatically affected by liquid storage, but was submitted to contrasted effect after cryopreservation. Finally, we investigated the potential involvement of several signaling pathways in initiation of the chicken AR and the results showed that the AR could be mediated by activation of the PKA, PI3K and ERK MAPK pathways.
Advisors/Committee Members: Blesbois, Elisabeth (thesis director).
Subjects/Keywords: Conservation in vitro
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APA (6th Edition):
Lemoine, M. (2009). La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2009TOUR4005
Chicago Manual of Style (16th Edition):
Lemoine, Manuela. “La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.” 2009. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 21, 2021.
http://www.theses.fr/2009TOUR4005.
MLA Handbook (7th Edition):
Lemoine, Manuela. “La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.” 2009. Web. 21 Jan 2021.
Vancouver:
Lemoine M. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2009. [cited 2021 Jan 21].
Available from: http://www.theses.fr/2009TOUR4005.
Council of Science Editors:
Lemoine M. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2009. Available from: http://www.theses.fr/2009TOUR4005
Universidade do Rio Grande do Sul
30.
Bernardi, Ana Paula Machado.
Análise química, avaliação da atividade antioxidante e obtenção de culturas in vitro de espécies de hypericum nativas do Rio Grande do Sul.
Degree: 2007, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/14812
► Aproximadamente vinte espécies do gênero Hypericum (Guttiferae) têm ocorrência natural no Brasil, e concentram-se principalmente na região Sul do País. Considerando a importância deste gênero…
(more)
▼ Aproximadamente vinte espécies do gênero Hypericum (Guttiferae) têm ocorrência natural no Brasil, e concentram-se principalmente na região Sul do País. Considerando a importância deste gênero como fonte de substâncias com variadas atividades biológicas, tais como analgésica, antidepressiva, antimicrobiana, antiviral, antiproliferativa, entre outras, o presente trabalho teve como objetivos analisar a constituição química e o potencial antioxidante de espécies de Hypericum, desenvolver protocolos para manutenção de algumas espécies nativas através de culturas de tecidos e células e validar metodologia para quantificação de benzopiranos em H. polyanthemum proveniente de cultivo a campo, in
vitro e aclimatizado. Utilizando-se métodos cromatográficos e espectroscópicos, foram isolados e identificados o ácido fenólico ácido 5-O-cafeoil-1-metoxi-quínico e os flavonóides 3,7-dimetil-quercetina, 3-Ometil- quercetina, I3,II8-biapigenina, guaijaverina, isoquercitrina e hiperosídeo, todos derivados da quercetina e obtidos da fração acetato de etila das partes aéreas de H. ternum. Ainda desta espécie, porém da fração n-hexano das raízes, obteve-se o derivado de floroglucinol uliginosina B. De H. myrianthum foram isolados e identificados os flavonóides quercetina e hiperosídeo, da fração metanólica das partes aéres, bem como os derivados de floroglucinol japonicina A e uliginosina B, fração n-hexano das raízes. Através do método colorimétrico de Folin-Ciocalteau, foram quantificados os teores de fenólicos totais das espécies H. caprifoliatum, H. carinatum, H. myrianthum e H. polyanthemum, verificando-se teores variando entre 37,40 a 228,36 mg EQ/g de extrato seco. Utilizando as técnicas de TRAP (Total Radical-trapping Antioxidant Parameter), ORAC-PGV (Oxygen Radical Absorbance Capacity) e reação bioautográfica e espectrofotométrica com radicais DPPH• (2,2 difenil-1-picril-hidrazil) evidenciou-se que extratos brutos metanólicos, e frações metanólicas e n-hexânicas das espécies H. caprifoliatum, H. carinatum H. myrianthum e H. polyanthemum, bem como produtos isolados de espécies de Hypericum nativas do Estado apresentam potencial antioxidante. A regeneração in
vitro foi obtida em meio Murashige & Skoog modificado (MΔ) para as espécies H. campestre, H. caprifoliatum, H. carinatum, H. myrianthum, H. polyanthemum e H. ternum utilizando diferentes combinações e concentrações dos reguladores de crescimento 6-benzilamino-purina (BAP), ácido naftaleno-acético (ANA) e ácido 2,4 dicloro-fenóxi-acético (2,4-D). Plântulas dessas espécies foram posteriormente aclimatizadas com sucesso, sendo cultivadas a campo. As espécies micropropagadas demonstraram perfis químicos qualitativamente similares aos apresentados pelas plantas desenvolvidas no campo, de modo que o cultivo in
vitro apresenta-se como uma alternativa interessante aos métodos convencionais de produção de biomassa para extração de metabólitos secundários bioativos. Culturas de calos foram estabelecidas em meio MΔ para H. myrianthum, H. polyanthemum e H. ternum, utilizando diferentes…
Advisors/Committee Members: Von Poser, Gilsane Lino.
Subjects/Keywords: Hypericum; Hypericum; Phenolic compounds; Guttiferae; Antioxidant activity; Atividade antioxidante; In vitro culture; Compostos fenólicos; Acclimatization; Benzopyrans
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APA (6th Edition):
Bernardi, A. P. M. (2007). Análise química, avaliação da atividade antioxidante e obtenção de culturas in vitro de espécies de hypericum nativas do Rio Grande do Sul. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/14812
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bernardi, Ana Paula Machado. “Análise química, avaliação da atividade antioxidante e obtenção de culturas in vitro de espécies de hypericum nativas do Rio Grande do Sul.” 2007. Thesis, Universidade do Rio Grande do Sul. Accessed January 21, 2021.
http://hdl.handle.net/10183/14812.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bernardi, Ana Paula Machado. “Análise química, avaliação da atividade antioxidante e obtenção de culturas in vitro de espécies de hypericum nativas do Rio Grande do Sul.” 2007. Web. 21 Jan 2021.
Vancouver:
Bernardi APM. Análise química, avaliação da atividade antioxidante e obtenção de culturas in vitro de espécies de hypericum nativas do Rio Grande do Sul. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2007. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10183/14812.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bernardi APM. Análise química, avaliação da atividade antioxidante e obtenção de culturas in vitro de espécies de hypericum nativas do Rio Grande do Sul. [Thesis]. Universidade do Rio Grande do Sul; 2007. Available from: http://hdl.handle.net/10183/14812
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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