subject:(Immunity)

Texas A&M University

1. Tan, Peng. DISSECTING THE ROLES OF WHIP-TRIM14-PPP6C IN INNATE ANTIVIRAL IMMUNITY AND MYELOID BECLIN1 IN TUMOR IMMUNITY.

Degree: PhD, Medical Sciences, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173576

► The innate immune system has the ability to tune the inflammatory environment by modulating pathogen-recognition receptors (PRRs) signaling, which can either induce oncogenic changes or… (more)

▼ The innate immune system has the ability to tune the inflammatory environment by modulating pathogen-recognition receptors (PRRs) signaling, which can either induce oncogenic changes or generate antitumor immunity through cross-talking with adaptive immunity. Thus, tight regulation of innate immune signaling pathways is essential either for an effective immune response against viral infections and tumor or for preventing detrimental autoimmunity. Mitochondrial antiviral-signaling protein MAVS acts as a central hub for RIG-I receptor proximal signal propagation. However, key components in the assembly of the MAVS mitochondrial platform that promote RIG-I mitochondrial localization and optimal activation are still largely undefined. Employing pooled RNAi and yeast two-hybrid screenings, we report that the mitochondrial adaptor protein TRIM14 provides a docking platform for the assembly of the mitochondrial signaling complex required for maximal activation of RIG-I-mediated signaling, consisting of WHIP and protein phosphatase PPP6C. Following viral infection, the ubiquitin-binding domain in WHIP bridges RIG-I with MAVS through the binding to polyUb chains of RIGI at lysine 164. ATPase domain in WHIP contributes to the stabilization of RIG-I-dsRNA interaction. Moreover, phosphatase PPP6C is responsible for RIG-I dephosphorylation. Together, our findings define the WHIP-TRIM14-PPP6C mitochondrial signalosome required for RIG-I-mediated innate antiviral immunity. Giving the embryonic lethality of Beclin1 (Becn1) homozygous mice, roles of myeloid Becn1 in cancer-associated inflammation and tumor immunity remain elusive. We demonstrate herein that mice with myeloid loss of Becn1 (Becn1^ΔM) show high risk of precursor (pre)-B cell lymphoma associated with neutrophilia. Becn1 deficiency stabilizes neutrophil MEKK3 independent of autophagy, resulting in the aberrant p38 activation and exaggerated tumorigenic inflammation that triggers the expressions of PD-L1 and pro-survival genes in pre-B cells. Importantly, we identify low Becn1 expression of neutrophils correlates with programmed death ligand 1 (PD-L1) levels and cancer recurrence in patients with pre-B acute lymphoblastic lymphoma (ALL). In a mouse model of melanoma, expansion and infiltration of neutrophils and B cells due to Becn1 deficiency favor metastasis of B16 melanoma. Thus, homeostatic maintenance of Becn1 level in neutrophils will contribute to the outcome of the tumorigenic process of pre-B cells. Advisors/Committee Members: Moczygemba, Margie (advisor), Wang, Rongfu (advisor), Zhou, Yubin (committee member), Cao, Qi (committee member), Xu, Yi (committee member).

Subjects/Keywords: Innate Immunity; Tumor Immunity

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APA (6^th Edition):

Tan, P. (2018). DISSECTING THE ROLES OF WHIP-TRIM14-PPP6C IN INNATE ANTIVIRAL IMMUNITY AND MYELOID BECLIN1 IN TUMOR IMMUNITY. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173576

Chicago Manual of Style (16^th Edition):

Tan, Peng. “DISSECTING THE ROLES OF WHIP-TRIM14-PPP6C IN INNATE ANTIVIRAL IMMUNITY AND MYELOID BECLIN1 IN TUMOR IMMUNITY.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 02, 2021. http://hdl.handle.net/1969.1/173576.

MLA Handbook (7^th Edition):

Tan, Peng. “DISSECTING THE ROLES OF WHIP-TRIM14-PPP6C IN INNATE ANTIVIRAL IMMUNITY AND MYELOID BECLIN1 IN TUMOR IMMUNITY.” 2018. Web. 02 Mar 2021.

Vancouver:

Tan P. DISSECTING THE ROLES OF WHIP-TRIM14-PPP6C IN INNATE ANTIVIRAL IMMUNITY AND MYELOID BECLIN1 IN TUMOR IMMUNITY. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1969.1/173576.

Council of Science Editors:

Tan P. DISSECTING THE ROLES OF WHIP-TRIM14-PPP6C IN INNATE ANTIVIRAL IMMUNITY AND MYELOID BECLIN1 IN TUMOR IMMUNITY. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173576

2. Torres, Celia Aurora Tiglao. DNA Immunization: Role of Target Site, Bone Marrow-Derived Cells and Secretion of Antigen in the Initiation of Immune Responses: A Dissertation.

Degree: Graduate School of Biomedical Sciences, Department of Molecular Genetics and Microbiology, 1998, U of Massachusetts : Med

URL: http://escholarship.umassmed.edu/gsbs_diss/293

► DNA immunization, or the use of antigen-expressing DNAs to raise immune responses, represents a novel approach to the study and manipulation of immune responses.… (more)

▼ DNA immunization, or the use of antigen-expressing DNAs to raise immune responses, represents a novel approach to the study and manipulation of immune responses. In this dissertation, we examine the role of antigen expression at the target site, the role of antigen presentation by bone marrow-derived cells, and the effect of secretion of antigen on DNA-raised responses in mice. Immunizations were conducted using either gene gun delivery of DNA to the epidermis or intramuscular (i.m.) saline injections. To examine the role of antigen expression at the target site, we excised target sites at different time points following immunization. We immunized with plasmid DNA expressing three different forms of antigens: influenza hemagglutinin H1, human growth hormone and influenza nucleoprotein NP (membrane-bound, secreted and intracellular, respectively). We hypothesized that antigen expression at the target site would be essential in initiating immune responses. We demonstrate here that the target site plays different roles in gene gun and i.m. immunizations. We found that the skin target site played an essential role in eliciting maximal antibody and cytotoxic T lymphocyte (CTL) responses by gene gun immunization, although low-level responses can be raised independent of the target site. In contrast, the muscle target site was not essential for eliciting maximal immune responses following i.m. immunization. We suggest that gene gun immunization results in transfection of keratinocytes and bone marrow-derived Langerhans cells at the target site, and these cells together initiate maximal responses. In i.m. immunizations, on the other hand, nonmuscle cells at distal sites, perhaps bone marrow-derived cells in lymphoid tissues, become transfected and are sufficient for initiation of maximal responses. We also examined the role of antigen presentation by bone marrow-derived cells in initiation of CTL responses to influenza NP following gene gun and i.m. immunization. We hypothesized that antigen presentation by bone marrow-derived cells would be involved in initiation of CTL responses. To test this hypothesis, irradiated F1 mice of MHC class I H-2^bxd haplotype were reconstituted with bone marrow from either H-2^b or H-2^d donors, creating two sets of bone marrow chimeric mice (H-2^b → H-2^bxd and H-2^d → H-2^bxd, respectively). We immunized the two sets of bone marrow chimeric mice and determined the MHC haplotype restriction of the induced CTL responses using H-2^b- or H-2^d-restricted peptides of NP. We found that the CTL responses initiated following gene gun and i.m. immunization were restricted to the haplotype of the bone marrow donor. In H-2^b→ H-2^bxd chimeric mice, CTL responses were restricted to H-2^b, while in H-2^d→ H-2^bxd chimeric mice, CTL responses were restricted to H-2^d. Thus, antigen presentation by bone marrow-derived cells, and not by skin…

Subjects/Keywords: Immunity

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APA (6^th Edition):

Torres, C. A. T. (1998). DNA Immunization: Role of Target Site, Bone Marrow-Derived Cells and Secretion of Antigen in the Initiation of Immune Responses: A Dissertation. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from http://escholarship.umassmed.edu/gsbs_diss/293

Chicago Manual of Style (16^th Edition):

Torres, Celia Aurora Tiglao. “DNA Immunization: Role of Target Site, Bone Marrow-Derived Cells and Secretion of Antigen in the Initiation of Immune Responses: A Dissertation.” 1998. Doctoral Dissertation, U of Massachusetts : Med. Accessed March 02, 2021. http://escholarship.umassmed.edu/gsbs_diss/293.

MLA Handbook (7^th Edition):

Torres, Celia Aurora Tiglao. “DNA Immunization: Role of Target Site, Bone Marrow-Derived Cells and Secretion of Antigen in the Initiation of Immune Responses: A Dissertation.” 1998. Web. 02 Mar 2021.

Vancouver:

Torres CAT. DNA Immunization: Role of Target Site, Bone Marrow-Derived Cells and Secretion of Antigen in the Initiation of Immune Responses: A Dissertation. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 1998. [cited 2021 Mar 02]. Available from: http://escholarship.umassmed.edu/gsbs_diss/293.

Council of Science Editors:

Torres CAT. DNA Immunization: Role of Target Site, Bone Marrow-Derived Cells and Secretion of Antigen in the Initiation of Immune Responses: A Dissertation. [Doctoral Dissertation]. U of Massachusetts : Med; 1998. Available from: http://escholarship.umassmed.edu/gsbs_diss/293

3. Davidovics, Sarah A. Low-grade inflammation, immune capacity, and cancer.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/37166

► Inflammation is a well-established etiological factor in carcinogenesis. The immune system may also have the capacity to identify and clear malignant cells in a process… (more)

▼ Inflammation is a well-established etiological factor in carcinogenesis. The immune system may also have the capacity to identify and clear malignant cells in a process known as tumor immunosurveillance. The objective of this dissertation is to examine these roles of host immunity in carcinogenesis in immunocompetent adults. Specifically, we quantified the risk of cancer incidence and mortality by circulating, pre-diagnostic levels of the white blood cell (WBC) subtypes, neutrophils, lymphocytes, monocytes, eosinophils, and basophils, and cytomegalovirus (CMV) IgG titer in two longitudinal cohorts, the Atherosclerosis Risk in Communities (ARIC), 1987-2008, and the third National Health and Nutrition Examination Survey (NHANES III), 1988-2011. Multivariate Cox analyses were used to estimate the hazard ratios (HR) and 95% confidence intervals (CI) of cancer incidence and mortality by levels of immune markers. High neutrophil count was associated with an increased risk of total (HR: 1.11, 95% CI: 1.00, 1.25) and lung (HR: 1.59, 95% CI: 1.12, 2.26) cancer incidence, and total (HR: 1.44, 95% CI: 1.22, 1.72), lung (HR: 1.66, 95% CI: 1.18, 2.33) and breast (HR: 2.09, 95% CI: 1.10, 3.97) cancer mortality. Among men, high lymphocyte count was associated with a reduced risk of cancer incidence, after excluding prostate cancer (HR: 0.75, 95% CI: 0.62, 0.91), and an increased risk of prostate cancer incidence (HR: 1.31, 95% CI: 1.03, 1.66). Among women, lymphocyte count was positively associated with cancer mortality (HR: 1.40, 95% CI: 1.07, 1.82). The presence of basophils in circulation was associated with a reduced risk of cancer incidence (HR: 0.93, 95% CI: 0.85, 1.01) and mortality (HR: 0.87, 95% CI: 0.76, 1.00). Adjustment for the other WBC subtypes did not appreciably alter these estimates. No associations were found for monocyte or eosinophil counts. Lastly, high CMV IgG antibody titer was associated with increased cancer mortality in black CMV seropositive persons (HR: 1.38, 95% CI: 1.02, 1.89), while no association was present in whites or Mexican Americans. Our findings of an association between circulating pre-diagnostic levels of neutrophils, lymphocytes, basophils, and CMV IgG and cancer incidence and mortality support a role for low-grade inflammation and subclinical immune suppression in carcinogenesis. Advisors/Committee Members: Wang, Mei-Cheng (advisor).

Subjects/Keywords: cancer; immunity

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APA (6^th Edition):

Davidovics, S. A. (2014). Low-grade inflammation, immune capacity, and cancer. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Davidovics, Sarah A. “Low-grade inflammation, immune capacity, and cancer.” 2014. Thesis, Johns Hopkins University. Accessed March 02, 2021. http://jhir.library.jhu.edu/handle/1774.2/37166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Davidovics, Sarah A. “Low-grade inflammation, immune capacity, and cancer.” 2014. Web. 02 Mar 2021.

Vancouver:

Davidovics SA. Low-grade inflammation, immune capacity, and cancer. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Mar 02]. Available from: http://jhir.library.jhu.edu/handle/1774.2/37166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Davidovics SA. Low-grade inflammation, immune capacity, and cancer. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Cheng, Cheng. Genetic and Biochemical Analysis of Innate Immunity in Arabidopsis thaliana.

Degree: 2013, Texas Digital Library

URL: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66710

► Perception of evolutionarily conserved pathogen-associated molecular patterns (PAMPs) elicits rapid and profound transcriptional reprogramming in hosts and activates defense to pathogen attack. The molecular signaling… (more)

▼ Perception of evolutionarily conserved pathogen-associated molecular patterns (PAMPs) elicits rapid and profound transcriptional reprogramming in hosts and activates defense to pathogen attack. The molecular signaling networks underlying this plant pattern-triggered immunity (PTI) remain fragmented. We identified a series of mutants with altered pFRK1::LUC activity were identified and named as Arabidopsis genes governing immune gene expression (aggie) through forward genetic screening. Map-based cloning identified Aggie1 as encoding Arabidopsis C-terminal domain (CTD) phosphatase-like 3 (CPL3), a homolog of yeast FCP1 phosphatase that dephosphorylates the CTD of RNA polymerase II (RNAPII) during the transcription cycle. MAMP perception induced a rapid and transient CTD phosphorylation in Arabidopsis, underlying the modulation of CTD phosphorylation dynamics controlling plant immune responsive gene expression. Aggie1/CPL3 specifically dephosphorylated Ser2 of the CTD in vivo and in vitro and preferentially interacted with phosphorylated CTD. Transcriptional analysis indicates that cpl3 showed overall enhanced flg22-mediated transcription responses. Thus, Aggie1 negatively regulates immune responsive gene expression essential for suppression of pathogen growth by modulating the phosphorylation status of RNAPII CTD. Cyclin-dependent kinases C (CDKC) functions as RNAPII kinases. Interestingly, we also found the silencing of cdkc1 and cdkc2 in wild type reduced flg22-mediated transcription responses and the plants were more susceptible to Pseudomonas syringae DC3000, suggesting their positive role in PAMP-triggered immunity. Temperature fluctuation is a key determinant for microbial invasion into the host and for host evasion of the microbe. In contrast to mammals that maintain constant body temperature, plant internal temperature oscillates on a daily basis. It remains elusive how plants operate inducible defenses in response to temperature fluctuation. We report that ambient temperature changes lead to pronounced shifts of two distinct plant immune responses: pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI). Plants preferentially activate ETI signaling at relatively lower temperatures (10~23???C), whereas they switch to PTI signaling at moderately elevated temperatures (23~32???C). The Arabidopsis arp6 and hta9hta11 mutants, phenocopying plants grown at the elevated temperatures, exhibit enhanced PTI and yet reduced ETI responses. As the secretion of bacterial effectors favors low temperatures whereas bacteria multiply vigorously at elevated temperatures accompanied with increased MAMP production, our findings suggest that temperature oscillation might have driven dynamic co-evolution of distinct plant immune signaling responding to pathogen physiological changes. Advisors/Committee Members: He, Ping (advisor).

Subjects/Keywords: Plant immunity

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APA (6^th Edition):

Cheng, C. (2013). Genetic and Biochemical Analysis of Innate Immunity in Arabidopsis thaliana. (Thesis). Texas Digital Library. Retrieved from http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cheng, Cheng. “Genetic and Biochemical Analysis of Innate Immunity in Arabidopsis thaliana.” 2013. Thesis, Texas Digital Library. Accessed March 02, 2021. http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cheng, Cheng. “Genetic and Biochemical Analysis of Innate Immunity in Arabidopsis thaliana.” 2013. Web. 02 Mar 2021.

Vancouver:

Cheng C. Genetic and Biochemical Analysis of Innate Immunity in Arabidopsis thaliana. [Internet] [Thesis]. Texas Digital Library; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cheng C. Genetic and Biochemical Analysis of Innate Immunity in Arabidopsis thaliana. [Thesis]. Texas Digital Library; 2013. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Biron, Bethany. The Role of Peptidyl arginine deiminase, type IV (PAD4) in the Pathology of Shock/Sepsis.

Degree: Pathobiology, 2017, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:733274/

► Sepsis is a life threatening condition that elicits a dysregulated and damaging immune response, which in turn leads to tissue damage and Multiple Organ Dysfunction… (more)

▼ Sepsis is a life threatening condition that elicits a dysregulated and damaging immune response, which in turn leads to tissue damage and Multiple Organ Dysfunction (MOD). Critically ill patients that have suffered from trauma, complex surgery, gastrointestinal bleeding, obstetrical bleeding, etc., have an increased risk of developing sepsis, which may progress to Acute Respiratory Distress Syndrome (ARDS), leading in turn to an increase in mortality. Neutrophils are thought to play a role in the immune dysfunction seen in both shock and sepsis, and have been implicated in causing bystander tissue damage and organ dysfunction. Neutrophil extracellular traps (NETs) are large webs of decondensed chromatin coated with antimicrobial proteins that are released into the extracellular space. They serve as a novel effector function, regulated by the enzyme Peptidyl arginine deiminase, type IV (PAD4). NETs have been identified as an important aspect of innate immunity, but have also been linked to excessive inflammation and tissue damage. Their role in sepsis and shock/sepsis, however, is poorly understood. This dissertation examines how PAD4 (an enzyme central to NET formation) plays a role in the pathology of sepsis. It also examines whether a predispositional insult, such as severe shock antecedent to septic challenge, is affected this enzyme. Here we demonstrate that inhibition of PAD4 reduces mortality seen in a mouse polymicrobial sepsis model (CLP), as well as in a mouse model of hemorrhagic shock and sepsis (Hem/CLP). Clinically we showed that there is evidence of increased NET activity in septic patients. Together, this implies that PAD4-mediated NET formation contributes to the morbidity and mortality associated with sepsis and shock/sepsis, and that PAD4 merits further exploration as a possible therapeutic target against the damaging pro-inflammatory response seen in polymicrobial sepsis and indirect ARDS. Advisors/Committee Members: Ayala, Alfred (Advisor), Reichner, Jonathan (Reader), Lefort, Craig (Reader), Heffernan, Daithi (Reader), Levy, Bruce (Reader).

Subjects/Keywords: Cellular immunity

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APA (6^th Edition):

Biron, B. (2017). The Role of Peptidyl arginine deiminase, type IV (PAD4) in the Pathology of Shock/Sepsis. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733274/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Biron, Bethany. “The Role of Peptidyl arginine deiminase, type IV (PAD4) in the Pathology of Shock/Sepsis.” 2017. Thesis, Brown University. Accessed March 02, 2021. https://repository.library.brown.edu/studio/item/bdr:733274/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Biron, Bethany. “The Role of Peptidyl arginine deiminase, type IV (PAD4) in the Pathology of Shock/Sepsis.” 2017. Web. 02 Mar 2021.

Vancouver:

Biron B. The Role of Peptidyl arginine deiminase, type IV (PAD4) in the Pathology of Shock/Sepsis. [Internet] [Thesis]. Brown University; 2017. [cited 2021 Mar 02]. Available from: https://repository.library.brown.edu/studio/item/bdr:733274/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Biron B. The Role of Peptidyl arginine deiminase, type IV (PAD4) in the Pathology of Shock/Sepsis. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733274/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

6. Thompson, William Carl. The effects of actinomycin D on the primary and secondary immune responses induced in vitro.

Degree: PhD, Biochemistry and Biophysics, 1970, Oregon State University

URL: http://hdl.handle.net/1957/45640

► In order to assess the importance of RNA synthesis in the induction and antibody-producing phases of immune responses, actinomycin D was used to inhibit the… (more)

▼ In order to assess the importance of RNA synthesis in the induction and antibody-producing phases of immune responses, actinomycin D was used to inhibit the primary and secondary in vitro immune responses of mouse spleen cells against heterologous erythrocytes. The presence of actinomycin D (0.1 or 0.125 μg /ml) throughout the entire four day culture period inhibited the development of plaque-forming cells (PFCs) in both primary and secondary responses by more than 99%. The same drug concentration, if added after 1, 2, or 3 days of culture, inhibited the number of PFCs on the fourth day by 81-99%. Utilizing cultures of spleen cells from mice already undergoing a secondary immune response, no stimulation of the PFC number upon a 24 hour treatment with actinomycin D (1.25 μg /ml) was found, unlike the observation of Harris (J. Experimental Medicine 127:675, 1968), with a similar system of cultured rabbit spleen cells. The treatment of these mouse spleen suspensions for 24 hours with actinomycin D (1.25 μg /ml) usually brought about a 90-95% decrease in the number of PFCs. For both the primary and secondary in vitro immune responses the sensitivity to actinomycin D treatment throughout the culture period was determined, A drug concentration of 10⁻³ μg/ml caused no inhibition in either type of response, but rather caused some stimulation of PFC numbers by the end of the fourth day of culture, The secondary response was inhibited by 43% and 98% by actinomycin D concentrations of 3x10⁻³ and 10⁻² μg /ml respectively, The primary response was inhibited by 95% and 99% by actinomycin D concentrations of 3x10⁻³ and 10⁻² μg /ml respectively, This sensitivity of the entire response is much greater than that reported by Uyeki and Llacer (Biochemical Pharmacology 18:948, 1969) for actinomycin D additions at a later stage of the response, The spleen cell suspension was separated into a macrophagerich fraction and a lymphocyte-rich fraction. Treatment of the macrophage population for one hour with actinomycin D (0.1 μg /ml), either during or before the addition of antigen, inhibited by 95% the immune response which resulted following removal of the drug and addition of the lymphocyte-rich population to reconstitute the original cell suspension. The results are discussed with respect to the possible events occurring during the response which would account for the observed sensitivity to actinomycin D. A suggestion is made that the extremely high sensitivity of the early phases of the immune response may be due to the need for synthesis of some very large RNA molecule. Advisors/Committee Members: Newburgh, Robert W. (advisor).

Subjects/Keywords: Immunity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thompson, W. C. (1970). The effects of actinomycin D on the primary and secondary immune responses induced in vitro. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/45640

Chicago Manual of Style (16^th Edition):

Thompson, William Carl. “The effects of actinomycin D on the primary and secondary immune responses induced in vitro.” 1970. Doctoral Dissertation, Oregon State University. Accessed March 02, 2021. http://hdl.handle.net/1957/45640.

MLA Handbook (7^th Edition):

Thompson, William Carl. “The effects of actinomycin D on the primary and secondary immune responses induced in vitro.” 1970. Web. 02 Mar 2021.

Vancouver:

Thompson WC. The effects of actinomycin D on the primary and secondary immune responses induced in vitro. [Internet] [Doctoral dissertation]. Oregon State University; 1970. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1957/45640.

Council of Science Editors:

Thompson WC. The effects of actinomycin D on the primary and secondary immune responses induced in vitro. [Doctoral Dissertation]. Oregon State University; 1970. Available from: http://hdl.handle.net/1957/45640

Baylor University

7. Chung, Cheng-Han, 1982-. The chromatin accessibility signature of aging in human blood leukocytes stem from CD8+ T cells.

Degree: PhD, Baylor University. Institute of Biomedical Studies., 2017, Baylor University

URL: http://hdl.handle.net/2104/10165

► Human aging is linked to changes in immune function that contribute to decreased responses to pathogens and increased systemic inflammation. Human aging is also associated… (more)

▼ Human aging is linked to changes in immune function that contribute to decreased responses to pathogens and increased systemic inflammation. Human aging is also associated with profound epigenetic changes across cell types and tissues. How these changes affect the aging –associated decline of the immune system is unknown. The Assay for Transposase Accessible Chromatin with sequencing technology (ATAC-seq) allowed us to study, at a system biology level, the open chromatin landscapes of human peripheral blood mononuclear cells (PBMCs), monocytes, purified B and T cell subsets from healthy young and healthy elderly individuals. We captured aging-associated epigenomic remodeling in PBMCs consisting of (1) systematic chromatin closing at promoters and enhancers targeting the T cell signaling and development and (2) chromatin opening, mostly at quiescent and repressed sites associated with cytotoxicity. Transcriptome profiling of the same individuals revealed gene expression changes concordant with epigenomic changes. Analysis of naïve and memory CD4+ and CD8+ T cell subsets demonstrated that the epigenomic signature of aging in PBMCs arises mostly from memory CD8+ T cells, indicating that aging differentially affects T cell epigenomes in a subset-specific manner. This study provides the first systems-level description of chromatin accessibility changes associated with immune aging in human PBMCs and T cell subsets. It revealed in PBMCs significant chromatin closing at promoters and enhancers, including at the IL7R locus and the IL-7 signaling pathway. Our study revealed individual-level variability in aging-associated chromatin remodeling and provided a systematic and modular tool for assessing deviations from chronological age. The open chromatin profiling of sorted T cell subsets, concluded that the chromatin “aging signature” captured in PBMCs, mostly stems from memory CD8+ T cells. The combined ATAC-seq/RNA-seq analyses uncovered epigenetic changes poised for expression changes and active noncoding elements (e.g., enhancers), both of which will be essential for understanding the regulatory mechanisms underlying immunosenescence. Nevertheless, ATAC-seq based open chromatin profiling is a straightforward approach to identify functional genomic regulatory regions, master regulators, and gene regulatory networks controlling complex in vivo processes. In our lab, ATAC-seq is utilized to understand the epigenetics differences in different immune cells and diseases. Advisors/Committee Members: Banchereau, Jacques. (advisor).

Subjects/Keywords: Aging. Immunity.

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APA (6^th Edition):

Chung, Cheng-Han, 1. (2017). The chromatin accessibility signature of aging in human blood leukocytes stem from CD8+ T cells. (Doctoral Dissertation). Baylor University. Retrieved from http://hdl.handle.net/2104/10165

Chicago Manual of Style (16^th Edition):

Chung, Cheng-Han, 1982-. “The chromatin accessibility signature of aging in human blood leukocytes stem from CD8+ T cells.” 2017. Doctoral Dissertation, Baylor University. Accessed March 02, 2021. http://hdl.handle.net/2104/10165.

MLA Handbook (7^th Edition):

Chung, Cheng-Han, 1982-. “The chromatin accessibility signature of aging in human blood leukocytes stem from CD8+ T cells.” 2017. Web. 02 Mar 2021.

Vancouver:

Chung, Cheng-Han 1. The chromatin accessibility signature of aging in human blood leukocytes stem from CD8+ T cells. [Internet] [Doctoral dissertation]. Baylor University; 2017. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2104/10165.

Council of Science Editors:

Chung, Cheng-Han 1. The chromatin accessibility signature of aging in human blood leukocytes stem from CD8+ T cells. [Doctoral Dissertation]. Baylor University; 2017. Available from: http://hdl.handle.net/2104/10165

University of Oxford

8. Brenna, Elena. Characterizing the specificity of T follicular helper cell repertoire in human peripheral blood and tonsil samples.

Degree: PhD, 2018, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:3a6abf6f-6a1a-426a-9643-d2349012d8d8 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770457

► T follicular helper cells (Tfh) are specialized CD4⁺ T cells, primarily localized in germinal centers (GCs) in secondary lymphoid organs. These cells play a key… (more)

Subjects/Keywords: Cellular immunity

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APA (6^th Edition):

Brenna, E. (2018). Characterizing the specificity of T follicular helper cell repertoire in human peripheral blood and tonsil samples. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:3a6abf6f-6a1a-426a-9643-d2349012d8d8 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770457

Chicago Manual of Style (16^th Edition):

Brenna, Elena. “Characterizing the specificity of T follicular helper cell repertoire in human peripheral blood and tonsil samples.” 2018. Doctoral Dissertation, University of Oxford. Accessed March 02, 2021. http://ora.ox.ac.uk/objects/uuid:3a6abf6f-6a1a-426a-9643-d2349012d8d8 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770457.

MLA Handbook (7^th Edition):

Brenna, Elena. “Characterizing the specificity of T follicular helper cell repertoire in human peripheral blood and tonsil samples.” 2018. Web. 02 Mar 2021.

Vancouver:

Brenna E. Characterizing the specificity of T follicular helper cell repertoire in human peripheral blood and tonsil samples. [Internet] [Doctoral dissertation]. University of Oxford; 2018. [cited 2021 Mar 02]. Available from: http://ora.ox.ac.uk/objects/uuid:3a6abf6f-6a1a-426a-9643-d2349012d8d8 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770457.

Council of Science Editors:

Brenna E. Characterizing the specificity of T follicular helper cell repertoire in human peripheral blood and tonsil samples. [Doctoral Dissertation]. University of Oxford; 2018. Available from: http://ora.ox.ac.uk/objects/uuid:3a6abf6f-6a1a-426a-9643-d2349012d8d8 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770457

Michigan State University

9. Moorhead, John W. A study of experimental conditions affecting the secondary immune response in vitro.

Degree: MS, Dept. of Microbiology and Public Health, 1966, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:14058

Subjects/Keywords: Immunity

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APA (6^th Edition):

Moorhead, J. W. (1966). A study of experimental conditions affecting the secondary immune response in vitro. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:14058

Chicago Manual of Style (16^th Edition):

Moorhead, John W. “A study of experimental conditions affecting the secondary immune response in vitro.” 1966. Masters Thesis, Michigan State University. Accessed March 02, 2021. http://etd.lib.msu.edu/islandora/object/etd:14058.

MLA Handbook (7^th Edition):

Moorhead, John W. “A study of experimental conditions affecting the secondary immune response in vitro.” 1966. Web. 02 Mar 2021.

Vancouver:

Moorhead JW. A study of experimental conditions affecting the secondary immune response in vitro. [Internet] [Masters thesis]. Michigan State University; 1966. [cited 2021 Mar 02]. Available from: http://etd.lib.msu.edu/islandora/object/etd:14058.

Council of Science Editors:

Moorhead JW. A study of experimental conditions affecting the secondary immune response in vitro. [Masters Thesis]. Michigan State University; 1966. Available from: http://etd.lib.msu.edu/islandora/object/etd:14058

University of Illinois – Urbana-Champaign

10. Granger, Kyle L. A pilot study on the effects of sow management practices on piglet immune responsiveness to weaning stress.

Degree: MS, Animal Sciences, 2016, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/95266

► The consequences of maternal prenatal stress exposure of the gestating sow on the developing immune system of her offspring is not well understood. There is… (more)

▼ The consequences of maternal prenatal stress exposure of the gestating sow on the developing immune system of her offspring is not well understood. There is limited data that primarily focuses on the health and well-being of the neonates of livestock animals, principally within the swine industry, in relation to the presence of unavoidable production stressors, including—but not limited to—weaning, mixing and social hierarchy. Each of the aforementioned stressors has been previously shown to affect the health, well-being, performance and productivity of sows as well as exacerbate the disease process by compromising the immune system. Thus, more information is needed to elucidate the complex relationship between prenatal stressors and postnatal immunological competence of the offspring. The objective of this thesis was to assess the effects of sow housing environment, social stress, and dietary fiber treatments during gestation on the immune and stress responsiveness of their progeny to weaning stress. Piglets were obtained from a larger-scale study of a 180 group-housed gestating sows. Briefly, sows were randomly allotted to 1 of 2 high fiber gestation diets (1) 30% wheat middlings and 15% soybean hulls (MID-SOY) or 30% distillers dried grains and 30% corn germ meal (DDG-GM) and to a group pen with feeding stalls of either 0.6 m (SHT) or 1.8 m (LNG) in length. Sows were fed dietary treatments starting on gestational d 35 and then moved to treatment pens at gestational d 37 and kept until d 104. On d 37, prior to moving into their experimental pens, a subsample of sows were subjected to a dominance test by which we determined a dominance value (DV). Those sows with a high DV were labeled dominant (DOM) and those with a low DV were labeled submissive (SUB). From both the larger study and the subsample of sows, 40-42 piglets were selected (balanced across treatments) based on body weight prior to weaning, with the two heaviest and two lightest piglets from each litter being used. All piglets were weaned at 19 ± 2 d-of-age. Blood samples were taken 24h prior to weaning, and then 7 and 14 days post-weaning to assess descriptive and functional aspects of both innate and adaptive immunity and cortisol. These data revealed that piglets weaned from sows fed MID-SOY during gestation had a profile indicative of a skewed TH1 (cell-mediated) response, while piglets weaned from sows fed DDG-GM diet were skewed toward TH2 (humoral) response. Piglets from sows housed in pens with LNG feeding stalls were better able to cope with weaning stress compared to those piglets from sows housed in pens with SHT. Moreover, sow social status differentially impact piglet immune responsiveness to weaning stress. Piglets weaned from SUB sows had a greater cell-mediated immune response which may have cost them in terms of performance because piglets from DOM sows had improved performance. These results show that: (1) feeding gestating sows high fiber diets can impact the development and growth of her offspring; (2) physical environment of gestating… Advisors/Committee Members: Salak-Johnson, Janeen L (advisor), Gaskins, Rex (committee member), Dailey, Megan J (committee member).

Subjects/Keywords: adaptive immunity; gestation; innate immunity; piglet; weaning

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APA (6^th Edition):

Granger, K. L. (2016). A pilot study on the effects of sow management practices on piglet immune responsiveness to weaning stress. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/95266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Granger, Kyle L. “A pilot study on the effects of sow management practices on piglet immune responsiveness to weaning stress.” 2016. Thesis, University of Illinois – Urbana-Champaign. Accessed March 02, 2021. http://hdl.handle.net/2142/95266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Granger, Kyle L. “A pilot study on the effects of sow management practices on piglet immune responsiveness to weaning stress.” 2016. Web. 02 Mar 2021.

Vancouver:

Granger KL. A pilot study on the effects of sow management practices on piglet immune responsiveness to weaning stress. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2142/95266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Granger KL. A pilot study on the effects of sow management practices on piglet immune responsiveness to weaning stress. [Thesis]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/95266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New Mexico

11. Sepahi, Ali. From olfaction to immunity: Characterization of nasal immunity in bony fish.

Degree: UNM Biology Department, 2018, University of New Mexico

URL: https://digitalrepository.unm.edu/biol_etds/277

► The olfactory system is a common route pathogen entry in vertebrates. As a consequence, the nasopharynx-associated lymphoid tissue (NALT) needs to rapidly clear infections… (more)

▼ The olfactory system is a common route pathogen entry in vertebrates. As a consequence, the nasopharynx-associated lymphoid tissue (NALT) needs to rapidly clear infections without compromising the sense of olfaction. NALT is present in teleost fish but its cellular and molecular mechanisms of action have not been investigated to this date. This dissertation focuses on three aims: 1. investigating the role of CCL19-like as a primordial chemokine in vertebrate nasal immunity, 2. determining the presence of tissue microenvironments within the olfactory organ (OO) of rainbow trout, and 3. understanding the immune contributions of olfactory sensory neurons (OSNs) in teleosts against viruses. In aim 1, we report six isoforms of CCL19-like chemokine in salmonids such as CK12. CK12 is mainly expressed in mucosal tissues and plays an important role in antiviral immunity. Although recombinant protein CK12 is not chemotactic in vitro, it induces infiltration of APCs and CD8⁺ T cells into OO of rainbow trout in vivo. In aim 2, we demonstrate the presence of two different microenvironments (mucosal tip and neuroepithelial) in the OO of rainbow trout. The tip of the epithelium harbors clusters of CD8α⁺ cells whereas few numbers are found in the neuroepithelium. CD8a⁺ cell localization corresponds with a higher expression of chemokine and chemokine receptors in the tip versus the neuroepithelial side. We report that cell proliferation in response to viral nasal delivery occurs mainly at the tip. In aim 3, we unravel for the first time a new function for OSNs by which nasal rhadboviruses induce apoptosis in crypt neurons, a type of OSN, in rainbow trout via the interaction of viral G glycoprotein and the neuron TrkA receptor. CD8α⁺ T cells infiltrate to the OO within minutes of nasal viral delivery and this response was abrogated when TrkA was blocked. Infiltrating CD8α⁺ T cells originated from the microvasculature surrounding the olfactory bulb (OB) and not the periphery. In conclusion, this dissertation provides the first tissue, cellular and molecular characterization of teleost NALT and reveals a novel function of vertebrate OSNs in eliciting rapid nasal anti-viral immune responses in the OO and OB. Advisors/Committee Members: Dr. Irene Salinas, Dr. Coenraad Adema, Dr. Judy Cannon, Dr. Jacques Robert.

Subjects/Keywords: Immunity; nasal; sensory neurons; teleost; Biology; Immunity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sepahi, A. (2018). From olfaction to immunity: Characterization of nasal immunity in bony fish. (Doctoral Dissertation). University of New Mexico. Retrieved from https://digitalrepository.unm.edu/biol_etds/277

Chicago Manual of Style (16^th Edition):

Sepahi, Ali. “From olfaction to immunity: Characterization of nasal immunity in bony fish.” 2018. Doctoral Dissertation, University of New Mexico. Accessed March 02, 2021. https://digitalrepository.unm.edu/biol_etds/277.

MLA Handbook (7^th Edition):

Sepahi, Ali. “From olfaction to immunity: Characterization of nasal immunity in bony fish.” 2018. Web. 02 Mar 2021.

Vancouver:

Sepahi A. From olfaction to immunity: Characterization of nasal immunity in bony fish. [Internet] [Doctoral dissertation]. University of New Mexico; 2018. [cited 2021 Mar 02]. Available from: https://digitalrepository.unm.edu/biol_etds/277.

Council of Science Editors:

Sepahi A. From olfaction to immunity: Characterization of nasal immunity in bony fish. [Doctoral Dissertation]. University of New Mexico; 2018. Available from: https://digitalrepository.unm.edu/biol_etds/277

Universiteit Utrecht

12. Fermie, J. Failure of Immunity Against Hepatitis C Virus: Implications of Defects in Early Immunity.

Degree: 2013, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/283340

► The hepatitis C virus (HCV) is an upcoming human pathogen. Although HCV infection is mostly asymptomatic, persistent infection may lead to liver failure or liver… (more)

Subjects/Keywords: HCV; Hepatitis C Virus; Adaptive immunity; Innate immunity; T cell; chronic infection; viral immunity; immunity

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APA (6^th Edition):

Fermie, J. (2013). Failure of Immunity Against Hepatitis C Virus: Implications of Defects in Early Immunity. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/283340

Chicago Manual of Style (16^th Edition):

Fermie, J. “Failure of Immunity Against Hepatitis C Virus: Implications of Defects in Early Immunity.” 2013. Masters Thesis, Universiteit Utrecht. Accessed March 02, 2021. http://dspace.library.uu.nl:8080/handle/1874/283340.

MLA Handbook (7^th Edition):

Fermie, J. “Failure of Immunity Against Hepatitis C Virus: Implications of Defects in Early Immunity.” 2013. Web. 02 Mar 2021.

Vancouver:

Fermie J. Failure of Immunity Against Hepatitis C Virus: Implications of Defects in Early Immunity. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Mar 02]. Available from: http://dspace.library.uu.nl:8080/handle/1874/283340.

Council of Science Editors:

Fermie J. Failure of Immunity Against Hepatitis C Virus: Implications of Defects in Early Immunity. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/283340

Mississippi State University

13. Moen, Eleanor Marie. Comparative analysis of the gut microbiome in wild and lab strains of Anopheles quadrimaculatus Say, and its effect on innate immunity.

Degree: MS, Agriculture and Life Sciences, College of, 2018, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06292018-154328/ ;

► Vector competence of mosquitoes has been linked to the conditions in which the larvae mature to adults. The microbial community obtained from the rearing environment… (more)

Subjects/Keywords: immunity; microbiome; Mosquito

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APA (6^th Edition):

Moen, E. M. (2018). Comparative analysis of the gut microbiome in wild and lab strains of Anopheles quadrimaculatus Say, and its effect on innate immunity. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-06292018-154328/ ;

Chicago Manual of Style (16^th Edition):

Moen, Eleanor Marie. “Comparative analysis of the gut microbiome in wild and lab strains of Anopheles quadrimaculatus Say, and its effect on innate immunity.” 2018. Masters Thesis, Mississippi State University. Accessed March 02, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-06292018-154328/ ;.

MLA Handbook (7^th Edition):

Moen, Eleanor Marie. “Comparative analysis of the gut microbiome in wild and lab strains of Anopheles quadrimaculatus Say, and its effect on innate immunity.” 2018. Web. 02 Mar 2021.

Vancouver:

Moen EM. Comparative analysis of the gut microbiome in wild and lab strains of Anopheles quadrimaculatus Say, and its effect on innate immunity. [Internet] [Masters thesis]. Mississippi State University; 2018. [cited 2021 Mar 02]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06292018-154328/ ;.

Council of Science Editors:

Moen EM. Comparative analysis of the gut microbiome in wild and lab strains of Anopheles quadrimaculatus Say, and its effect on innate immunity. [Masters Thesis]. Mississippi State University; 2018. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06292018-154328/ ;

University of Alberta

14. Guntermann, Silvia. Caspase activity and regulation in Drosophila melanogaster innate immunity.

Degree: PhD, Department of Medical Microbiology and Immunology, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/cr207tp490

► The fine balance struck between life and death is key for the health of all multicellular organisms and therefore the pathways that govern life and… (more)

▼ The fine balance struck between life and death is key for the health of all multicellular organisms and therefore the pathways that govern life and death decisions are evolutionarily highly conserved. For example, the human TNF and the Drosophila IMD pathways coordinate signaling elements such as conserved JNK, NF-κB, and caspase modules to drive appropriate responses. Caspases are an evolutionarily conserved family of cysteinyl aspartate proteases with fundamental roles in the opposing processes of cell-survival and cell-death. The caspase Dredd is an essential pro-survival regulator of the IMD mediated immune response. However, the detailed involvement of Dredd in the sequential activation of the IMD cascade, the position of Dredd, and Dredd’s interactions with the IMD pathway were unknown. In addition, the molecular mechanism of Dredd activation and regulation was unclear. In this study I conducted a thorough structural and functional analysis of the mechanism of Dredd activity in IMD signaling. I revealed numerous interactions of Dredd with early activators of the IMD pathway. I showed that the caspase activity inhibitor p35 blocked Dredd activation in a mechanism that is distinct from the general mechanism of p35- dependent caspase inhibition. In addition, Dredd activation appears independent of auto-processing which might be explained by the lack of Dredd linker residues that support auto-processing and may explain Dredd’s sole function in pro- survival responses. In a combination of cell culture and in vivo assays, I demonstrated that Dredd is required for the activation of dJNK signaling upstream of dTAK1 and determined that Dredd additionally functions down- stream of Imd activation. Furthermore, my data uncovered a dual regulation of dIAP2 by RING domain mediated destruction and Dronc mediated N-terminal proteolytic cleavage. In summary, the data indicate a distinct activation mechanism for Dredd and establish dual functions for Dredd in the IMD signaling cascade, where Dredd is required in a proximal signaling complex for the early transduction of a phospho-relay to Rel and dJNK as well as for the subsequent activation of Rel. The data also uncover a novel role for Dronc in immune signaling. Combined the findings underline the importance and complexity of non-apoptotic roles of caspases.

Subjects/Keywords: innate immunity; caspase

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APA (6^th Edition):

Guntermann, S. (2014). Caspase activity and regulation in Drosophila melanogaster innate immunity. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cr207tp490

Chicago Manual of Style (16^th Edition):

Guntermann, Silvia. “Caspase activity and regulation in Drosophila melanogaster innate immunity.” 2014. Doctoral Dissertation, University of Alberta. Accessed March 02, 2021. https://era.library.ualberta.ca/files/cr207tp490.

MLA Handbook (7^th Edition):

Guntermann, Silvia. “Caspase activity and regulation in Drosophila melanogaster innate immunity.” 2014. Web. 02 Mar 2021.

Vancouver:

Guntermann S. Caspase activity and regulation in Drosophila melanogaster innate immunity. [Internet] [Doctoral dissertation]. University of Alberta; 2014. [cited 2021 Mar 02]. Available from: https://era.library.ualberta.ca/files/cr207tp490.

Council of Science Editors:

Guntermann S. Caspase activity and regulation in Drosophila melanogaster innate immunity. [Doctoral Dissertation]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cr207tp490

Cornell University

15. Mouchka, Morgan. Microbial Friends And Foes: Characterizing The Cnidarian Response To Pathogenic And Mutualistic Microorganisms.

Degree: PhD, Ecology, 2014, Cornell University

URL: http://hdl.handle.net/1813/36127

► The ecology and evolution of cnidarians is driven by symbiotic and pathogenic hostmicrobe relationships. Research regarding these relationships is especially timely given the recent decline… (more)

▼ The ecology and evolution of cnidarians is driven by symbiotic and pathogenic hostmicrobe relationships. Research regarding these relationships is especially timely given the recent decline of coral reef ecosystems, in part due to disruptions in cnidarian-microbe interactions. My dissertation is an experimental analysis of how cnidarians respond to both harmful and beneficial microorganisms and explores the interplay between these two types of interactions. Corals provide a multifaceted habitat that supports a rich bacterial assemblage, and in Chapter 1, I review our current knowledge regarding the diversity, specificity, development, and functions of these assemblages. With a meta-analysis of previous work, I quantitatively analyze what is known regarding the relationship between coral-associated microorganisms and disease. Finally, I examine evidence that these populations could be disrupted by climatic change. One of the most well-known mutualistic relationships is that between cnidarians and unicellular dinoflagellates. To evaluate the molecular mechanisms that underlie the persistence of this relationship, in Chapter 2, I identify differentially expressed transcripts between symbiotic and aposymbiotic individuals of the model sea anemone, Aiptasia pallida. These transcripts include those with potential functions in several metabolic pathways, transport of nutrients between the partners, and host tolerance of the dinoflagellate. To broaden our understanding of the cnidarian response to pathogenic microbes, in Chapter 3, I report the host transcriptome response of aposymbiotic Aiptasia to experimental inoculation with Serratia marcescens. My results suggest that Aiptasia responds to bacterial challenge via the regulation of tumor necrosis factor receptor-associated factor-mediated signaling, apoptosis, and ubiquitination, thus suggesting that lower metazoans respond to immune challenge via highly conserved mechanisms. To determine how the Aiptasia immune response is modulated via the presence of dinoflagellate symbionts, in Chapter 4, I compared gene expression and behavioral assays of S. marcescens-exposed anemones with and without their symbionts. The presence of dinoflagellates greatly alters the number and type of genes expressed in response to bacterial challenge. In addition, symbiotic anemones were less likely to recover from pathogen exposure and had lower survival rates than their aposymbiotic counterparts. These results are consistent with the hypothesis that symbiotic dinoflagellates suppress Aiptasia immunity, perhaps to promote symbiotic homeostasis. Advisors/Committee Members: Harvell, Catherine Drew (chair), Lazzaro, Brian (committee member), Searle, Angela E. (committee member), (committee member).

Subjects/Keywords: cnidarian; immunity; microorganisms

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APA (6^th Edition):

Mouchka, M. (2014). Microbial Friends And Foes: Characterizing The Cnidarian Response To Pathogenic And Mutualistic Microorganisms. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/36127

Chicago Manual of Style (16^th Edition):

Mouchka, Morgan. “Microbial Friends And Foes: Characterizing The Cnidarian Response To Pathogenic And Mutualistic Microorganisms.” 2014. Doctoral Dissertation, Cornell University. Accessed March 02, 2021. http://hdl.handle.net/1813/36127.

MLA Handbook (7^th Edition):

Mouchka, Morgan. “Microbial Friends And Foes: Characterizing The Cnidarian Response To Pathogenic And Mutualistic Microorganisms.” 2014. Web. 02 Mar 2021.

Vancouver:

Mouchka M. Microbial Friends And Foes: Characterizing The Cnidarian Response To Pathogenic And Mutualistic Microorganisms. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1813/36127.

Council of Science Editors:

Mouchka M. Microbial Friends And Foes: Characterizing The Cnidarian Response To Pathogenic And Mutualistic Microorganisms. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36127

University of Cambridge

16. Kaewert, Sarah. Immune-driven positive and balancing selection in human populations.

Degree: PhD, 2019, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/293003

► This thesis examines the evidence for positive and balancing selection on immune genes in 388 individuals grouped into thirteen geographically diverse populations. The data are… (more)

▼ This thesis examines the evidence for positive and balancing selection on immune genes in 388 individuals grouped into thirteen geographically diverse populations. The data are high coverage whole genome sequences, many from populations that have been sparsely represented in global genetic diversity studies in the past. Two main analyses were performed for both positive and balancing selection: enrichment tests for each population and class of immune genes, and filtering for top variants or genes driving selection signals. Four different measures of positive selection and three measures of balancing selection were used to scan the whole genome data for evidence of selection. Further filters, including functional importance predictors, were used to filter results for potential driver variants. Positive selection results show significant enrichment for genes associated with bacteria or virus interaction, the innate immune system, and antigen processing and presentation. Results also include variants potentially driving signals of selection. One of these is a missense variant in the Northeast Siberian population in the gene IL27, which is involved in modulation of immune response to infection. Balancing selection enrichment tests show that genes associated with T cell function and antigen processing and presentation are significantly enriched in every population. The HLA region features heavily in these enrichments. One top gene result is GNLY, a gene that produces the antimicrobial protein granulysin, in the West Siberian and Island Southeast Asian populations. Another is PGLYRP4, which is a top gene in seven populations and is involved in recognition and defense against Gram-negative and -positive bacteria. In conclusion, as a general trend there is more enrichment in genes that interact with bacteria and viruses in the positive selection results, and more enrichment in genes involved with antigen processing and presentation and T cell function in the balancing selection results. The combined results show the different immune-driven selection histories of each population, as well as highlight a number of variants and genes that are potential drivers of selection and promising candidates for further study.

Subjects/Keywords: selection; immunity; genetics

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APA (6^th Edition):

Kaewert, S. (2019). Immune-driven positive and balancing selection in human populations. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/293003

Chicago Manual of Style (16^th Edition):

Kaewert, Sarah. “Immune-driven positive and balancing selection in human populations.” 2019. Doctoral Dissertation, University of Cambridge. Accessed March 02, 2021. https://www.repository.cam.ac.uk/handle/1810/293003.

MLA Handbook (7^th Edition):

Kaewert, Sarah. “Immune-driven positive and balancing selection in human populations.” 2019. Web. 02 Mar 2021.

Vancouver:

Kaewert S. Immune-driven positive and balancing selection in human populations. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Mar 02]. Available from: https://www.repository.cam.ac.uk/handle/1810/293003.

Council of Science Editors:

Kaewert S. Immune-driven positive and balancing selection in human populations. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/293003

University of Florida

17. Brice, David C. Innate Antiviral Defenses of Oral Epithelial Cells.

Degree: PhD, Medical Sciences - Immunology and Microbiology (IDP), 2018, University of Florida

URL: https://ufdc.ufl.edu/UFE0054013

► With many viruses spread via saliva, oral epithelial cells (OECs) act as one of the first barriers against infection of these pathogens. However, the defenses… (more)

Subjects/Keywords: immunity – peptide – virus

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APA (6^th Edition):

Brice, D. C. (2018). Innate Antiviral Defenses of Oral Epithelial Cells. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0054013

Chicago Manual of Style (16^th Edition):

Brice, David C. “Innate Antiviral Defenses of Oral Epithelial Cells.” 2018. Doctoral Dissertation, University of Florida. Accessed March 02, 2021. https://ufdc.ufl.edu/UFE0054013.

MLA Handbook (7^th Edition):

Brice, David C. “Innate Antiviral Defenses of Oral Epithelial Cells.” 2018. Web. 02 Mar 2021.

Vancouver:

Brice DC. Innate Antiviral Defenses of Oral Epithelial Cells. [Internet] [Doctoral dissertation]. University of Florida; 2018. [cited 2021 Mar 02]. Available from: https://ufdc.ufl.edu/UFE0054013.

Council of Science Editors:

Brice DC. Innate Antiviral Defenses of Oral Epithelial Cells. [Doctoral Dissertation]. University of Florida; 2018. Available from: https://ufdc.ufl.edu/UFE0054013

University of Cambridge

18. Kaewert, Sarah. Immune-driven positive and balancing selection in human populations.

Degree: PhD, 2019, University of Cambridge

URL: https://doi.org/10.17863/CAM.40154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774826

► This thesis examines the evidence for positive and balancing selection on immune genes in 388 individuals grouped into thirteen geographically diverse populations. The data are… (more)

▼ This thesis examines the evidence for positive and balancing selection on immune genes in 388 individuals grouped into thirteen geographically diverse populations. The data are high coverage whole genome sequences, many from populations that have been sparsely represented in global genetic diversity studies in the past. Two main analyses were performed for both positive and balancing selection: enrichment tests for each population and class of immune genes, and filtering for top variants or genes driving selection signals. Four different measures of positive selection and three measures of balancing selection were used to scan the whole genome data for evidence of selection. Further filters, including functional importance predictors, were used to filter results for potential driver variants. Positive selection results show significant enrichment for genes associated with bacteria or virus interaction, the innate immune system, and antigen processing and presentation. Results also include variants potentially driving signals of selection. One of these is a missense variant in the Northeast Siberian population in the gene IL27, which is involved in modulation of immune response to infection. Balancing selection enrichment tests show that genes associated with T cell function and antigen processing and presentation are significantly enriched in every population. The HLA region features heavily in these enrichments. One top gene result is GNLY, a gene that produces the antimicrobial protein granulysin, in the West Siberian and Island Southeast Asian populations. Another is PGLYRP4, which is a top gene in seven populations and is involved in recognition and defense against Gram-negative and -positive bacteria. In conclusion, as a general trend there is more enrichment in genes that interact with bacteria and viruses in the positive selection results, and more enrichment in genes involved with antigen processing and presentation and T cell function in the balancing selection results. The combined results show the different immune-driven selection histories of each population, as well as highlight a number of variants and genes that are potential drivers of selection and promising candidates for further study.

Subjects/Keywords: selection; immunity; genetics

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APA (6^th Edition):

Kaewert, S. (2019). Immune-driven positive and balancing selection in human populations. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.40154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774826

Chicago Manual of Style (16^th Edition):

Kaewert, Sarah. “Immune-driven positive and balancing selection in human populations.” 2019. Doctoral Dissertation, University of Cambridge. Accessed March 02, 2021. https://doi.org/10.17863/CAM.40154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774826.

MLA Handbook (7^th Edition):

Kaewert, Sarah. “Immune-driven positive and balancing selection in human populations.” 2019. Web. 02 Mar 2021.

Vancouver:

Kaewert S. Immune-driven positive and balancing selection in human populations. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Mar 02]. Available from: https://doi.org/10.17863/CAM.40154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774826.

Council of Science Editors:

Kaewert S. Immune-driven positive and balancing selection in human populations. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://doi.org/10.17863/CAM.40154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774826

Rutgers University

19. Pierre, Kamau, 1987-. Evaluation of the role of cortisol in modulating seasonal changes in immunity.

Degree: PhD, Biomedical Engineering, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55618/

►

Inflammatory diseases such as asthma, rheumatoid arthritis (RA) and systemic vasculitis exhibit seasonality with higher prevalence and aggravated symptoms occurring during the winter months. Though… (more)

▼

Inflammatory diseases such as asthma, rheumatoid arthritis (RA) and systemic vasculitis exhibit seasonality with higher prevalence and aggravated symptoms occurring during the winter months. Though the underlying causes remain to be elucidated, disease seasonality has been associated with circannual changes in antibodies, hormonal levels, acute phase reactants and numerous immunomodulatory components. For instance, RA risk biomarkers such as IL-6R mRNA, sIL-6 receptor and C-reactive proteins vary significantly throughout the year with peak expressions during the winter season. Apart from seasonal variation, the dynamics of functions such as cytokine production and hormone secretion display diurnal fluctuations that are entrained to the external 24-hour light/dark cycle. The light fraction of the light/dark cycle, or photoperiod, varies predictably throughout the year and is a robust environmental cue that synchronizes seasonal variations in neuroendocrine function. It is well established that cortisol, regulated via hypothalamic-pituitary-adrenal (HPA) axis activity and entrained to the external photoperiod, is a potent immunomodulator whose level varies seasonally. A detailed experimental analysis of the role of the HPA axis in regulating seasonal changes in immune function is lacking due to the complex network of biological interactions. In this work, we developed a semi-mechanistic mathematical model to evaluate cortisol’s role in modulating seasonal immunological plasticity. Our results indicate a shift from an anti- to a pro-inflammatory state as the seasons progress from summer through winter with elevated expression of the cortisol-activating enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), an inflammatory disease biomarker, predicted for the winter season. Experimentally, the pro-inflammatory phenotype correlated with mitotic asynchrony is mitigated following glucocorticoid-induced cell resynchronization. A cortisol-dependent, time-of-day and seasonal variability in the synchronization of the molecular clock and cell cycle was predicted. These findings have major health implications as the misalignment of internal dynamics with environmental signals has been associated with inflammatory disease progression. The current model provides a framework for exploring the impact of asynchrony between the circadian and cell cycle oscillators, amongst cells in a population, on immune system dynamics. Knowledge of the underlying putative mechanisms governing seasonal and diurnal modifications of immune system dynamics can be applied to design more effective preventative and chronotherapeutic strategies addressing the development and advancement of a pro-inflammatory state.

Advisors/Committee Members: Androulakis, Ioannis P (chair), Hacihaliloglu, Ilker (internal member), Shinbrot, Troy (internal member), Schlesinger, Naomi (outside member), School of Graduate Studies.

Subjects/Keywords: Cortisol; Immunity system

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APA (6^th Edition):

Pierre, Kamau, 1. (2017). Evaluation of the role of cortisol in modulating seasonal changes in immunity. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55618/

Chicago Manual of Style (16^th Edition):

Pierre, Kamau, 1987-. “Evaluation of the role of cortisol in modulating seasonal changes in immunity.” 2017. Doctoral Dissertation, Rutgers University. Accessed March 02, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/55618/.

MLA Handbook (7^th Edition):

Pierre, Kamau, 1987-. “Evaluation of the role of cortisol in modulating seasonal changes in immunity.” 2017. Web. 02 Mar 2021.

Vancouver:

Pierre, Kamau 1. Evaluation of the role of cortisol in modulating seasonal changes in immunity. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2021 Mar 02]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55618/.

Council of Science Editors:

Pierre, Kamau 1. Evaluation of the role of cortisol in modulating seasonal changes in immunity. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55618/

20. Evans, John W., III. Itk is a Dual Action Regulator of Immunoreceptor Signaling in the Innate and Adaptive Immune System: A Dissertation.

Degree: Immunology and Microbiology, Pathology, 2013, U of Massachusetts : Med

URL: http://escholarship.umassmed.edu/gsbs_diss/688

► The cells and molecules that comprise the immune system are essential for mounting an effective response against microbes. A successful immune response limits pathology… (more)

▼ The cells and molecules that comprise the immune system are essential for mounting an effective response against microbes. A successful immune response limits pathology within the host while simultaneously eliminating the pathogen. The key to this delicate balance is the correct recognition of the pathogen and the appropriate response of immune cells. Cellular activation originates through receptors that relay information about the state of the microenvironment to different compartments within the cell. The rapid relay of information is called signal transduction and employs a network of signaling mediators such as kinases, phosphatases, adaptor molecules, and transcription factors. IL-2 inducible T cell kinase (Itk) is a non-receptor tyrosine kinase that is an integral component of signal transduction downstream of many immunoreceptors. This dissertation describes two distinct pathways that utilize Itk in both phases of the immune response. T cells use the TCR to sense a multitude of peptide-based ligands and to transmit signals inside the cell to activate cellular function. In this regard, the diversity of ligands the T cells encounter can be portrayed as analog inputs. Once a critical threshold is met, signaling events transpire in close proximity to the plasma membrane to activate major downstream pathways in the cell. The majority of these pathways are digital in nature resulting in the on or off activation of T cells. We find, however, that altering the TCR signal strength that a T cell receives can result in an analog-based response. Here, the graded expression of a transcription factor, IRF4, is modulated through the activity of Itk. We link this graded response to an NFAT-mediated pathway in which the digital vs. analog nature has been previously uncharacterized. Finally, we demonstrate that the repercussions of an analog signaling pathway is the altered expression of a second transcription factor, Eomes, which is important in the differentiation and function of T cells. These results suggest that Itk is crucial in the modulation of TCR signal strength. Mast cells primarily rely on the IgE-bound FcεR1 for pathogen recognition. Crosslinking this receptor activates mast cells and results in degranulation and cytokine production via an expansive signaling cascade. Upon stimulation, Itk is recruited to the plasma membrane and phosphorylated. Little else is known about how Itk operates inside of mast cells. We find that mast cells lacking Itk are hyperresponsive to FcεR1-mediated activation. This is most apparent in the amount of IL-4 and IL-13 produced in comparison to wild-type mast cells. Increased cytokine production was accompanied by elevated and sustained signaling downstream of the FcεR1. Finally, biochemical evidence demonstrates that Itk is part of an inhibitory complex containing the phosphatase SHIP-1. These results indicate a novel function for Itk as a negative regulator in FcεR1- mediated mast cell activation. Advisors/Committee Members: Leslie J. Berg, PhD.

Subjects/Keywords: Signal Transduction; Protein-Tyrosine Kinases; Adaptive Immunity; Innate Immunity; Immunity; Immunopathology

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APA (6^th Edition):

Evans, John W., I. (2013). Itk is a Dual Action Regulator of Immunoreceptor Signaling in the Innate and Adaptive Immune System: A Dissertation. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from http://escholarship.umassmed.edu/gsbs_diss/688

Chicago Manual of Style (16^th Edition):

Evans, John W., III. “Itk is a Dual Action Regulator of Immunoreceptor Signaling in the Innate and Adaptive Immune System: A Dissertation.” 2013. Doctoral Dissertation, U of Massachusetts : Med. Accessed March 02, 2021. http://escholarship.umassmed.edu/gsbs_diss/688.

MLA Handbook (7^th Edition):

Evans, John W., III. “Itk is a Dual Action Regulator of Immunoreceptor Signaling in the Innate and Adaptive Immune System: A Dissertation.” 2013. Web. 02 Mar 2021.

Vancouver:

Evans, John W. I. Itk is a Dual Action Regulator of Immunoreceptor Signaling in the Innate and Adaptive Immune System: A Dissertation. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2013. [cited 2021 Mar 02]. Available from: http://escholarship.umassmed.edu/gsbs_diss/688.

Council of Science Editors:

Evans, John W. I. Itk is a Dual Action Regulator of Immunoreceptor Signaling in the Innate and Adaptive Immune System: A Dissertation. [Doctoral Dissertation]. U of Massachusetts : Med; 2013. Available from: http://escholarship.umassmed.edu/gsbs_diss/688

21. Ho, Eric Chun Hei. A Simple Animal Model for Characterizing Gene Reglatory Control of an Immune Response.

Degree: PhD, 2016, University of Toronto

URL: http://hdl.handle.net/1807/73011

► A robust but tightly regulated system of innate gut immunity is required for maintaining gut homeostasis. Given the complex nature of the mammalian gut, a… (more)

▼ A robust but tightly regulated system of innate gut immunity is required for maintaining gut homeostasis. Given the complex nature of the mammalian gut, a simple yet phylogenetically relevant model is useful to decipher the underlying molecular controls that sustain this balance. Here I use the purple sea urchin larva as a morphologically simple model for these studies. I approach this problem with three interrelated strategies: (1) Characterization of larval immunocytes based on morphology, cell behaviour and gene expression, (2) analysis of gene activity in a model of gut-associated immune response to bacteria and (3) regulatory analysis of selected response genes. I characterize five larval cell types that are active in gut immunity and develop a gut associated infection model using the bacterium Vibrio diazotrophicus. RNA-seq analysis of this model is used to identify immune genes that are differentially expressed over the course of infection. Member of the sea urchin IL-17 multigene family are identified as immune response genes. One subfamily, SpIL-17-I, is expressed early upon exposure to V. diazotrophicus in the mid- and hindgut and is then attenuated. BAC-based GFP transgenes and smaller reporter constructs recapitulate endogenous SpIL-17-I expression. Regulatory regions are defined upstream and downstream of the IL-17 coding sequence. Deletions of critical elements results in a decrease or absence of IL-17 expression but never ectopic expression or expression in the absence of immune stimulus. Downstream function of IL-17 was investigated by perturbing its receptors using Morpholino antisense oligonucleotides. In addition to physiological changes in the larva, expression of several immune effector genes are downregulated in IL-17R perturbed embryos. Collectively this work suggests that a core network of regulatory circuitry is shared with the vertebrates in the gut associated immune response even in this simple larval form. This can then form the basis for more elaborate network analysis of gut immunity in future studies using this model. Advisors/Committee Members: Rast, Jonathan P, Medical Biophysics.

Subjects/Keywords: comparative immunity; gut immunity; IL-17; innate immunity; sea urchin; 0982

12 more images…

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APA (6^th Edition):

Ho, E. C. H. (2016). A Simple Animal Model for Characterizing Gene Reglatory Control of an Immune Response. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/73011

Chicago Manual of Style (16^th Edition):

Ho, Eric Chun Hei. “A Simple Animal Model for Characterizing Gene Reglatory Control of an Immune Response.” 2016. Doctoral Dissertation, University of Toronto. Accessed March 02, 2021. http://hdl.handle.net/1807/73011.

MLA Handbook (7^th Edition):

Ho, Eric Chun Hei. “A Simple Animal Model for Characterizing Gene Reglatory Control of an Immune Response.” 2016. Web. 02 Mar 2021.

Vancouver:

Ho ECH. A Simple Animal Model for Characterizing Gene Reglatory Control of an Immune Response. [Internet] [Doctoral dissertation]. University of Toronto; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1807/73011.

Council of Science Editors:

Ho ECH. A Simple Animal Model for Characterizing Gene Reglatory Control of an Immune Response. [Doctoral Dissertation]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/73011

University of Georgia

22. Rolon, Ariel. Salmonella vaccination studies in breeders and their progeny.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/22495

► Salmonella are ubiquitous enteric bacteria and potential pathogens, some affecting a wide host species range. Eradication of poultry-specific Salmonella from commercial flocks resulted in increased… (more)

▼ Salmonella are ubiquitous enteric bacteria and potential pathogens, some affecting a wide host species range. Eradication of poultry-specific Salmonella from commercial flocks resulted in increased prevalence of serovars with a wider host range in poultry and consequently increased potential of human food-borne disease due to consumption of poultry products. Regulatory Hazard Analysis Critical Control Points has been implemented with the goal of reducing Salmonella prevalence in poultry meat. Management intervention strategies to curb the occurrence of Salmonella have begun to look at competitive exclusion, killed and live Salmonella vaccines during production. Our studies focused on establishing how combined vaccination programs protect breeders through rearing and production, and if maternal antibody and competitive exclusion is protective for the progeny. Antibody response was measured at the systemic and mucosal humoral level and an infection model established to relate antibody response to actual bacterial prevalence. Day-of-age breeder vaccination increased intestinal IgG at 3 but not 10 weeks of age. Crop IgA and IgG, as well as gut and serum IgG peaks were observed after killed vaccines delivered at 11 and 17 weeks of age. An approximate 0.8 Log reduction in Salmonella counts due to live 1 and 21-day vaccine applications were obtained at 3 and 6, but not 11 weeks of age. By week 22 all vaccination programs reduced Salmonella counts by approximately 1.3 Log. High maternal antibody throughout production passed to the progeny failed to reduce Salmonella counts, whereas competitive exclusion consistently reduced Salmonella counts by approximately 1.4 Log. Maternal intestinal IgG transferred to the progeny was observed up to 13 days, but no interference of maternal antibody on the effectiveness of day-of-age live vaccination was detected. Day-of-age live vaccine reduced Salmonella counts at 3 and 13 but not 34 days of age, indicating that more than one live vaccine is necessary for prolonged protection during rearing. Live vaccine protection is probably a combined effect of humoral, cell-mediated intestinal immunity, and a competitive exclusion effect. Competitive exclusion and vaccination programs will reduce but not eliminate the incidence of Salmonella, and therefore should constitute complementary and not substitutive tools to integral biosecurity programs.

Subjects/Keywords: Salmonella; Challenge; Vaccine; Humoral Immunity; Mucosal Immunity; Maternal Immunity.

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APA (6^th Edition):

Rolon, A. (2014). Salmonella vaccination studies in breeders and their progeny. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22495

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rolon, Ariel. “Salmonella vaccination studies in breeders and their progeny.” 2014. Thesis, University of Georgia. Accessed March 02, 2021. http://hdl.handle.net/10724/22495.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rolon, Ariel. “Salmonella vaccination studies in breeders and their progeny.” 2014. Web. 02 Mar 2021.

Vancouver:

Rolon A. Salmonella vaccination studies in breeders and their progeny. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10724/22495.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rolon A. Salmonella vaccination studies in breeders and their progeny. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22495

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Schrum, Jacob E. Pathways Involved in Recognition and Induction of Trained Innate Immunity by Plasmodium falciparum.

Degree: PhD, Department of Medicine, Division of Infectious Diseases and Immunology, 2017, U of Massachusetts : Med

URL: http://escholarship.umassmed.edu/gsbs_diss/917

► Malarial infection in naïve individuals induces a robust innate immune response, but our understanding of the mechanisms by which the innate immune system recognizes… (more)

Subjects/Keywords: Plasmodium falciparum; innate immunity; innate immune memory; cGAS; trained immunity; Immunity; Immunology of Infectious Disease

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APA (6^th Edition):

Schrum, J. E. (2017). Pathways Involved in Recognition and Induction of Trained Innate Immunity by Plasmodium falciparum. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from http://escholarship.umassmed.edu/gsbs_diss/917

Chicago Manual of Style (16^th Edition):

Schrum, Jacob E. “Pathways Involved in Recognition and Induction of Trained Innate Immunity by Plasmodium falciparum.” 2017. Doctoral Dissertation, U of Massachusetts : Med. Accessed March 02, 2021. http://escholarship.umassmed.edu/gsbs_diss/917.

MLA Handbook (7^th Edition):

Schrum, Jacob E. “Pathways Involved in Recognition and Induction of Trained Innate Immunity by Plasmodium falciparum.” 2017. Web. 02 Mar 2021.

Vancouver:

Schrum JE. Pathways Involved in Recognition and Induction of Trained Innate Immunity by Plasmodium falciparum. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2017. [cited 2021 Mar 02]. Available from: http://escholarship.umassmed.edu/gsbs_diss/917.

Council of Science Editors:

Schrum JE. Pathways Involved in Recognition and Induction of Trained Innate Immunity by Plasmodium falciparum. [Doctoral Dissertation]. U of Massachusetts : Med; 2017. Available from: http://escholarship.umassmed.edu/gsbs_diss/917

Universiteit Utrecht

24. Hogenkamp, A. Collectins in innate immune defense of pigs and chickens.

Degree: 2007, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/23210

► In the Netherlands, the number of chickens and pigs exceeds the number of human inhabitants by far. Therefore, combating infections in livestock is not only… (more)

▼ In the Netherlands, the number of chickens and pigs exceeds the number of human inhabitants by far. Therefore, combating infections in livestock is not only of economical importance, but also of great importance to public health. The innate immune system provides a first line defense against infectious pathogens, and its effector mechanisms rely on the recognition of features that are common to many pathogens. These features, known as pathogen-associated molecular patterns can be recognized by pattern recognition molecules at the site of infection, which is often an epithelial surface like the lung, skin or gastro-intestinal tract. Collectins, an important group of pattern recognition molecules, have been reported to be expressed in many epithelial surfaces. In the lung, Surfactant Protein-D (SP-D) contributes to the maintenance of a sterile respiratory system. Its role in the gastro-intestinal tract, which is dominated by the presence of micro-organisms, is less clear. Therefore, we investigated the interaction of porcine SP-D (pSP-D) with various Gram-negative bacteria isolated from the porcine intestine. It was shown that while pSP-D is capable of reducing growth rates of E. coli K12, the growth rates and survival of pathogenic bacterial strains isolated from the porcine intestine remained unaffected. Furthermore, pSP-D was shown to affect bacterial adhesion- and invasion- characteristics, leading to a significant increase in adhesion and invasion of bacteria into IPI-I2 cells. It is not yet clear what the mechanism behind these effects is, but it could reflect a scavenger function for pSP-D in the intestine. In the chicken, only two collectins had been described thus far. In this thesis, we report the discovery of three new chicken collectins, chicken Collectin 1-3 (cCL-1, -2,and -3) and one chicken lectin, chicken Lung Lectin (cLL). Results from this study support the assumption that cCL-1, -2 and -3, together with their respective mammalian homologues CL-L1, CL-K1 and CL-P1 represent three new unique classes within the collectin protein family. Because of its predominant expression in the chicken respiratory tract, the newly discovered chicken lectin was designated chicken Lung Lectin (cLL). To investigate whether these chicken (col)lectins are involved in avian innate immunity, their mRNA expression levels during bacterial and viral infections were investigated. Samples derived from three infection-models were analyzed, and the results showed that mRNA expression of chicken (col)lectins cCL-1, cCL-2, cSP-A and cLL can be affected by both bacterial and viral infections. We also investigated the structural and functional aspects of cLL. To this end, recombinant cLL was successfully produced in HEK-293 EBNA cells. Characterization of recombinant cLL revealed that this protein is capable of binding sugars, and, as predicted from its sequence, does so in a calcium-dependent manner. In addition, cLL showed anti-viral activity against avian IAV in our preliminary tests, and HAA-inhibition of a human isolate of IAV…

Subjects/Keywords: Diergeneeskunde; collectin; innate immunity; chicken

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APA (6^th Edition):

Hogenkamp, A. (2007). Collectins in innate immune defense of pigs and chickens. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/23210

Chicago Manual of Style (16^th Edition):

Hogenkamp, A. “Collectins in innate immune defense of pigs and chickens.” 2007. Doctoral Dissertation, Universiteit Utrecht. Accessed March 02, 2021. http://dspace.library.uu.nl:8080/handle/1874/23210.

MLA Handbook (7^th Edition):

Hogenkamp, A. “Collectins in innate immune defense of pigs and chickens.” 2007. Web. 02 Mar 2021.

Vancouver:

Hogenkamp A. Collectins in innate immune defense of pigs and chickens. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2007. [cited 2021 Mar 02]. Available from: http://dspace.library.uu.nl:8080/handle/1874/23210.

Council of Science Editors:

Hogenkamp A. Collectins in innate immune defense of pigs and chickens. [Doctoral Dissertation]. Universiteit Utrecht; 2007. Available from: http://dspace.library.uu.nl:8080/handle/1874/23210

25. 舟見, 健児. Toll-like receptor 3の細胞内局在、及びシグナル伝達に関与する機能ドメインの同定 : The cytoplasmic "linker region" in Toll-like receptor 3 controls receptor licalization and signaling; Toll-like receptor 3 ノサイボウナイキョクザイオヨビシグナルデンタツニカンヨスルキノウドメインノドウテイ.

Degree: Nara Institute of Science and Technology / 奈良先端科学技術大学院大学

URL: http://hdl.handle.net/10061/2831

Subjects/Keywords: Innate immunity

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APA (6^th Edition):

舟見, �. (n.d.). Toll-like receptor 3の細胞内局在、及びシグナル伝達に関与する機能ドメインの同定 : The cytoplasmic "linker region" in Toll-like receptor 3 controls receptor licalization and signaling; Toll-like receptor 3 ノサイボウナイキョクザイオヨビシグナルデンタツニカンヨスルキノウドメインノドウテイ. (Thesis). Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10061/2831

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

舟見, 健児. “Toll-like receptor 3の細胞内局在、及びシグナル伝達に関与する機能ドメインの同定 : The cytoplasmic "linker region" in Toll-like receptor 3 controls receptor licalization and signaling; Toll-like receptor 3 ノサイボウナイキョクザイオヨビシグナルデンタツニカンヨスルキノウドメインノドウテイ.” Thesis, Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Accessed March 02, 2021. http://hdl.handle.net/10061/2831.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

舟見, 健児. “Toll-like receptor 3の細胞内局在、及びシグナル伝達に関与する機能ドメインの同定 : The cytoplasmic "linker region" in Toll-like receptor 3 controls receptor licalization and signaling; Toll-like receptor 3 ノサイボウナイキョクザイオヨビシグナルデンタツニカンヨスルキノウドメインノドウテイ.” Web. 02 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

舟見 �. Toll-like receptor 3の細胞内局在、及びシグナル伝達に関与する機能ドメインの同定 : The cytoplasmic "linker region" in Toll-like receptor 3 controls receptor licalization and signaling; Toll-like receptor 3 ノサイボウナイキョクザイオヨビシグナルデンタツニカンヨスルキノウドメインノドウテイ. [Internet] [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; [cited 2021 Mar 02]. Available from: http://hdl.handle.net/10061/2831.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

舟見 �. Toll-like receptor 3の細胞内局在、及びシグナル伝達に関与する機能ドメインの同定 : The cytoplasmic "linker region" in Toll-like receptor 3 controls receptor licalization and signaling; Toll-like receptor 3 ノサイボウナイキョクザイオヨビシグナルデンタツニカンヨスルキノウドメインノドウテイ. [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; Available from: http://hdl.handle.net/10061/2831

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Utah

26. Bareyan, Diana H. Studies of vaccine adjuvants that can elicit the generation of mucosal immune responses to cutaneously delivered antigens;.

Degree: MS;, Pathology;, 2007, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/410/rec/1263

► Vaccines are designed to protect against infectious diseases. The majority of commercially available vaccines are administered to hosts via parenteral routes and provoke the development… (more)

▼ Vaccines are designed to protect against infectious diseases. The majority of commercially available vaccines are administered to hosts via parenteral routes and provoke the development of only systemic immunity. However, most pathogens infect susceptible hosts by crossing a mucosal barrier. The development of vaccines capable of eliciting both systemic and mucosal immune responses would be desirable, and provide better protection to immunized hosts. Our laboratory previously reported that the bioactive form of vitamin D3, la25(OH)2D3, when added to vaccines and administered cutaneously, induces both systemic and mucosal immune responses. It was established that antigen-laden dendritic cells (DCs) can bypass draining lymph node (LN) sequestration and localize to classical inductive sites of mucosal immunity when exposed to la25(OH)2D3 during their maturation. In Chapter 2 of this thesis, we demonstrate that la25(OH)2D3-exposed skin residing DCs can bypass the draining LN and localize to various non-draining LNs and Peyer's patches (PPs). The responsible mechanism involves a temporal suppression in the activation-induced upregulation of CCR7, resulting in a depressed chemotaxis toward CCL21 (a ligand for CCR7). The suppression is transient and normal chemotactic activities are required over time. Pathogen recognition is achieved in part through Toll-like receptors (TLRs) that are members of the pattern-recognition receptor family. Innate immune responses that are initiated by TLR activation can influence subsequent events and regulate the types of adaptive immune responses that develop. In Chapter 3 of this thesis, natural ligands for TLR3, TLR4 and TLR9 were examined for possible roles as adjuvants in the generation of systemic and mucosal immune responses. DCs activated with TLR3 or TLR4 ligands were able to upregulate the expression of 25-hydroxycholecalciferol-la-hydroxylase, an enzyme that catalyzes the conversion of bioinactive 25-hydroxyvitamin D3 to the active la25(OH)2D3. Also, TLR3/4-activated DCs exhibited altered migratory properties in vivo through a la25(OH)2D3 -dependent processes. Our results suggest that the successful generation of mucosal immunity to antigens administered cutaneously, might best be achieved when endogenously produced, or exogenously administered lot25(OH)2D3, is available.

Subjects/Keywords: Myeloid Dendritic Cell; Muscosal Immunity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bareyan, D. H. (2007). Studies of vaccine adjuvants that can elicit the generation of mucosal immune responses to cutaneously delivered antigens;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/410/rec/1263

Chicago Manual of Style (16^th Edition):

Bareyan, Diana H. “Studies of vaccine adjuvants that can elicit the generation of mucosal immune responses to cutaneously delivered antigens;.” 2007. Masters Thesis, University of Utah. Accessed March 02, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/410/rec/1263.

MLA Handbook (7^th Edition):

Bareyan, Diana H. “Studies of vaccine adjuvants that can elicit the generation of mucosal immune responses to cutaneously delivered antigens;.” 2007. Web. 02 Mar 2021.

Vancouver:

Bareyan DH. Studies of vaccine adjuvants that can elicit the generation of mucosal immune responses to cutaneously delivered antigens;. [Internet] [Masters thesis]. University of Utah; 2007. [cited 2021 Mar 02]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/410/rec/1263.

Council of Science Editors:

Bareyan DH. Studies of vaccine adjuvants that can elicit the generation of mucosal immune responses to cutaneously delivered antigens;. [Masters Thesis]. University of Utah; 2007. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/410/rec/1263

University of Utah

27. Zhang, Tian Yi. Programming of immune responsiveness by end-organ metabolism of glucocorticoids.

Degree: PhD, Pathology;, 2007, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/20/rec/936

► 11beta-Hydroxysteroid Dehydrogenase Type 1 (11betaHSD1) catalyzes the conversion of C11-keto-glucocorticoids (GCs) to bioactive C11-hydroxy-GCs, thereby generating activating ligands for the glucocorticoid receptor. We demonstrate that… (more)

▼ 11beta-Hydroxysteroid Dehydrogenase Type 1 (11betaHSD1) catalyzes the conversion of C11-keto-glucocorticoids (GCs) to bioactive C11-hydroxy-GCs, thereby generating activating ligands for the glucocorticoid receptor. We demonstrate that immune cells of both lymphoid (immature thymocytes, CD4+/CD8+ T cells and B220+ B cells) and myeloid (CD11b+ macrophages (M-phi-s). CD11c+ dendrite cells) lineages possess 11betaHSD1 reductase activity. Activation of wild-type, but not 11betaHSD1-/- CD4+ T cells in the presence of C11-keto-GCs attenuates IFN-gamma and IL-2 production. The ability of 11betaHSD1+/+ CD4+ T cells to generate bioactive GCs via intracellular 11betaHSD1 allows a degree of autonomous control over lymphocyte biology and effector functions. Mice deficient in 11betaHSD1 exhibit a proinflammatory phenotype in vivo, as evidenced by the accelerated weight loss and elevated serum TNFalpha, IL-12p40 levels following LPS challenge. 11betaHSD1-/- splenic M-phi-s (splnM-phi-s) and B cells contribute to the inflammatory phenotype since they over express inflammatory cytokines following LPS stimulation. 11betaHSD1+/+ and 11betaHSD1-/- bone marrow derived M-phi-s (BMM-phi-s) acquire and inflammatory phenotype only following conditioning with low levels of GCs, suggesting the hyperresponsiveness of 11betaHSD1-/- splinM-phi-s does not result from intrinsic 11betaHSD1 deficiency. Instead, the proinflammatory phenotype of 11betaHSD1-/- splnM-phi-s results from differential differentiation within the 11betaHSD1-/- host microenvironment, when plasma GC levels are moderately elevated. The overproduction of inflammatory cytokines by GC-conditioned BMM-phi-s and 11betaHSD1-/- splnM-phi-s appears to result from increased activation of the NFkB and MAP kinase signaling cascades, and an attenuation of P13K-dependent Akt activation. Additionally, 11betaHSD1-/- splnM-phi-s and GC conditioned BMM-phi-s express significantly more Src-homology 2 containing 5’ phosphatase 1 (SHIP1), which appears to be responsible for the depression in Akt activation. The presence of neutralizing antibodies for TGFbeta abrogates the ability of GCs to enhance SHIP1 expression in BMM-phi-s, suggesting GCs upregulate SHIP1 expression via induction to TGF-beta expression. Consistently, cultures of GC-conditioned BMM-phi-s and CD11b- immune cells obtained from the BM and spleen 11betaHSD1-/- mice express increased levels of bioactive TGFbeta. Our results suggest that modest elevations in micro-environmental GC levels can modify M-phi differentiation and thereby influence subsequent responses to stimulation.

Subjects/Keywords: immunity; Metabolism; Immue Response

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, T. Y. (2007). Programming of immune responsiveness by end-organ metabolism of glucocorticoids. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/20/rec/936

Chicago Manual of Style (16^th Edition):

Zhang, Tian Yi. “Programming of immune responsiveness by end-organ metabolism of glucocorticoids.” 2007. Doctoral Dissertation, University of Utah. Accessed March 02, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/20/rec/936.

MLA Handbook (7^th Edition):

Zhang, Tian Yi. “Programming of immune responsiveness by end-organ metabolism of glucocorticoids.” 2007. Web. 02 Mar 2021.

Vancouver:

Zhang TY. Programming of immune responsiveness by end-organ metabolism of glucocorticoids. [Internet] [Doctoral dissertation]. University of Utah; 2007. [cited 2021 Mar 02]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/20/rec/936.

Council of Science Editors:

Zhang TY. Programming of immune responsiveness by end-organ metabolism of glucocorticoids. [Doctoral Dissertation]. University of Utah; 2007. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/20/rec/936

University of California – Irvine

28. Morse, Robert Paul. Structural and functional characterization of contact-dependent growth inhibition systems.

Degree: Biological Sciences, 2014, University of California – Irvine

URL: http://www.escholarship.org/uc/item/61x733nv

► Bacteria have developed complex mechanisms to thrive in their environments. Contact- dependent growth inhibition (CDI), a novel inter-bacterial competition mechanism was recently discovered in gram-negative… (more)

▼ Bacteria have developed complex mechanisms to thrive in their environments. Contact- dependent growth inhibition (CDI), a novel inter-bacterial competition mechanism was recently discovered in gram-negative bacteria, and is used to block growth of neighboring cells. The CDI growth inhibitory activity is contained in the C-terminal domain of CdiA effector proteins (CdiA- CT, toxin), and is delivered into target bacterial cells. All CDI+ bacteria also produce small CdiI immunity proteins that protect them from autoinhibition. CdiA-CT and CdiI from different species exhibit high sequence variability, which predicts diverse structures and/or interaction interfaces. Here we describe the initial structural characterization of the CDI system, providing functional insights into distinct CDI mechanisms.We have solved X-ray crystal structures of CDI toxin/immunity complexes from Escherichia coli (EC869), Burkholderia pseudomallei (1026b), and Enterobacter cloacae (ECL), representing three distinct families of CDI proteins. The interfaces between the toxin/immunity pairs are distinct, providing important insights into CdiA-CT/CdiI binding specificity. The EC869 CdiA-CT/CdiI binding interface is especially intriguing and consists of a unique β-augmentation interaction, by which CdiA-CT^EC869 inserts a short β-hairpin structural element into a cavity on the surface of CdiI^EC869. This interface is conserved among EC869 family CdiA-CT/CdiI complexes, but heterologous toxin and immunity proteins within this family do not interact with high affinity. Additionally, the EC869, 1026b, and ECL toxin structures helped elucidate and characterize three distinct CdiA-CT growth-inhibiting activities. EC869 and 1026b CdiA-CTs are structurally similar and contain DNase and tRNase activities, respectively. In contrast, ECL CdiA-CT shares no structural homology with EC869 or 1026b CdiA-CTs, but is structurally and functionally similar to colicin E3, a toxin with rRNase activity from a distinct bacterial system. We also report the crystal structure of the CdiA-CT toxin from uropathogenic E. coli strain 536 in complex with its permissive factor, CysK, representing a unique step in CDI. The complex formed mimics the interaction found in the cysteine synthase complex. Finally, we solved the structure of a domain from the large, non-toxin region of E. coli CdiA, providing a starting point towards future research into the CdiA-CT cleavage and translocation mechanisms.

Subjects/Keywords: Biochemistry; Biology; Immunity; Structure; Toxin

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APA (6^th Edition):

Morse, R. P. (2014). Structural and functional characterization of contact-dependent growth inhibition systems. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/61x733nv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Morse, Robert Paul. “Structural and functional characterization of contact-dependent growth inhibition systems.” 2014. Thesis, University of California – Irvine. Accessed March 02, 2021. http://www.escholarship.org/uc/item/61x733nv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Morse, Robert Paul. “Structural and functional characterization of contact-dependent growth inhibition systems.” 2014. Web. 02 Mar 2021.

Vancouver:

Morse RP. Structural and functional characterization of contact-dependent growth inhibition systems. [Internet] [Thesis]. University of California – Irvine; 2014. [cited 2021 Mar 02]. Available from: http://www.escholarship.org/uc/item/61x733nv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Morse RP. Structural and functional characterization of contact-dependent growth inhibition systems. [Thesis]. University of California – Irvine; 2014. Available from: http://www.escholarship.org/uc/item/61x733nv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

29. Aggrey, Angela Akosua. A Novel Protective Role for Platelet-Mediated Immune Responses in Murine Experimental Cerebral Malaria.

Degree: PhD, 2013, University of Rochester

URL: http://hdl.handle.net/1802/27239

► Cerebral malaria is caused by complications from Plasmodium falciparum infection and claims hundreds of thousands of lives of young children in endemic countries annually. Platelets… (more)

Subjects/Keywords: Platelets; Innate Immunity, Malaria; Inflammation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aggrey, A. A. (2013). A Novel Protective Role for Platelet-Mediated Immune Responses in Murine Experimental Cerebral Malaria. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/27239

Chicago Manual of Style (16^th Edition):

Aggrey, Angela Akosua. “A Novel Protective Role for Platelet-Mediated Immune Responses in Murine Experimental Cerebral Malaria.” 2013. Doctoral Dissertation, University of Rochester. Accessed March 02, 2021. http://hdl.handle.net/1802/27239.

MLA Handbook (7^th Edition):

Aggrey, Angela Akosua. “A Novel Protective Role for Platelet-Mediated Immune Responses in Murine Experimental Cerebral Malaria.” 2013. Web. 02 Mar 2021.

Vancouver:

Aggrey AA. A Novel Protective Role for Platelet-Mediated Immune Responses in Murine Experimental Cerebral Malaria. [Internet] [Doctoral dissertation]. University of Rochester; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1802/27239.

Council of Science Editors:

Aggrey AA. A Novel Protective Role for Platelet-Mediated Immune Responses in Murine Experimental Cerebral Malaria. [Doctoral Dissertation]. University of Rochester; 2013. Available from: http://hdl.handle.net/1802/27239

Universiteit Utrecht

30. Kamp, M.E. Immunity to schistosomes in man and snail.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/301207

► Schistosomiasis is a parasitic disease found in tropical areas, caused by fluke worms of the genus Schistosoma (S.). This parasite can infect humans and other… (more)

Subjects/Keywords: Schistosomiasis; immunity; resistance; FREP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kamp, M. E. (2014). Immunity to schistosomes in man and snail. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/301207

Chicago Manual of Style (16^th Edition):

Kamp, M E. “Immunity to schistosomes in man and snail.” 2014. Masters Thesis, Universiteit Utrecht. Accessed March 02, 2021. http://dspace.library.uu.nl:8080/handle/1874/301207.

MLA Handbook (7^th Edition):

Kamp, M E. “Immunity to schistosomes in man and snail.” 2014. Web. 02 Mar 2021.

Vancouver:

Kamp ME. Immunity to schistosomes in man and snail. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Mar 02]. Available from: http://dspace.library.uu.nl:8080/handle/1874/301207.

Council of Science Editors:

Kamp ME. Immunity to schistosomes in man and snail. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/301207

Open Access Theses and Dissertations