Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for subject:(Immune recognition 60). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters

1. Otali, Dennis. The combined effect of formalin fixation and individual steps in tissue processing on immunorecognition.

Degree: MS, 2007, University of Alabama – Birmingham

Because of many unanswered questions in this area, we investigated the effects of formalin fixation and the steps in tissue processing on immunohistochemical detection of specific antigen-antibody combinations of two cell cycle markers; proliferating cell nuclear antigen (PCNA) and Ki67/MIB-1. As a model to study these effects, we used cultured cells fixed in 10% neutral buffered formalin (NBF) alone and fixed in 10% NBF plus separately, the alcohols, xylene and paraffin used in each individual step in tissue processing. We found that in staining for PCNA, a proliferation antigen requires some fixation but that fixation with 10% NBF does not strongly affect the immunohistochemical signal for PCNA, except for fixation times longer than 48h, after which the signal decreases. The steps in tissue processing do decrease the intensity of PCNA staining, specifically the hydrophobic environment created by xylene. After antigen retrieval, staining for PCNA is not affected by the length of fixation but is decreased somewhat by dehydration at absolute ethanol and xylene during tissue processing. The Ki67/MIB-1 antibody recognizes and binds to the Ki67 epitope in cells. The frequency of this binding is a measure of cell proliferation in all active phases of the cell cycle (G1, S, G2 and M phase). While there is some decrease in the immunohistochemical signal with fixation in 10% NBF, dehydration and the development of a hydrophobic environment during tissue processing causes most of the decrease in Ki67/MIB-1 signal. After antigen retrieval, staining for Ki67/MIB-1 is decreased following periods of fixation in 10% NBF for longer than 24h. Staining for Ki67 after antigen retrieval is also decreased by dehydration during tissue processing especially the hydrophobic environment xylene. These results indicate that both fixation and the steps of tissue processing - dehydration through absolute ethanol and hydrophobic environment in xylene - affect epitope recognition by specific antibodies.

M.S.

xi, 69 p. : ill., digital, PDF file.

Biology

Natural Sciences and Mathematics

Formalin Fixation Tissue processing Immunohistochemistry Antigen retrieval

UNRESTRICTED

Advisors/Committee Members: Grizzle, William E., Watts, Stephen A.<br>, Manne, Upender.

Subjects/Keywords: Diagnostic immunohistochemistry <; br>; Cancer  – Immunodiagnosis <; br>; Antigen-antibody reactions <; br>; Immune recognition <; br>; Antigenic determinants <; br>; Antigens  – Antagonists <; br>; Formaldehyde <; br>; Fixation (Histology)<; br>; Matrix effect

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Otali, D. (2007). The combined effect of formalin fixation and individual steps in tissue processing on immunorecognition. (Masters Thesis). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,258

Chicago Manual of Style (16th Edition):

Otali, Dennis. “The combined effect of formalin fixation and individual steps in tissue processing on immunorecognition.” 2007. Masters Thesis, University of Alabama – Birmingham. Accessed September 18, 2019. http://contentdm.mhsl.uab.edu/u?/etd,258.

MLA Handbook (7th Edition):

Otali, Dennis. “The combined effect of formalin fixation and individual steps in tissue processing on immunorecognition.” 2007. Web. 18 Sep 2019.

Vancouver:

Otali D. The combined effect of formalin fixation and individual steps in tissue processing on immunorecognition. [Internet] [Masters thesis]. University of Alabama – Birmingham; 2007. [cited 2019 Sep 18]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,258.

Council of Science Editors:

Otali D. The combined effect of formalin fixation and individual steps in tissue processing on immunorecognition. [Masters Thesis]. University of Alabama – Birmingham; 2007. Available from: http://contentdm.mhsl.uab.edu/u?/etd,258

.