subject:(IgG)

NSYSU

1. Shih, Jen-Ching. none.

Degree: Master, Chemistry, 2003, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901103-135319

Subjects/Keywords: QCM; IgG

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APA (6^th Edition):

Shih, J. (2003). none. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901103-135319

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shih, Jen-Ching. “none.” 2003. Thesis, NSYSU. Accessed July 17, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901103-135319.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shih, Jen-Ching. “none.” 2003. Web. 17 Jul 2019.

Vancouver:

Shih J. none. [Internet] [Thesis]. NSYSU; 2003. [cited 2019 Jul 17]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901103-135319.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shih J. none. [Thesis]. NSYSU; 2003. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901103-135319

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. LIMA, Fernanda Cristina Gomes de. Análise da ação do meropenem e Polimixina E com a IgG humana frente isolados de Pseudomonas aeruginosa provenientes de infecções relacionadas à Assistência à Saúde .

Degree: 2015, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/14079

► Pseudomonas aeruginosa é uma importante causa de infecções apresentando, no Brasil, altas taxas de morbidade e mortalidade. Os principais mecanismos de resistência utilizados por P.… (more)

▼ Pseudomonas aeruginosa é uma importante causa de infecções apresentando, no Brasil, altas taxas de morbidade e mortalidade. Os principais mecanismos de resistência utilizados por P. aeruginosa são a produção de β-lactamases e a expressão de bombas de efluxo. O uso de IgG e antibióticos para tratamento destas infecções tem apresentado resultados bem sucedidos, o que motiva a realização de estudos quanto a atividade in vitro da IgG humana com antibióticos frente a isolados de P. aeruginosa. O objetivo deste estudo foi detectar a presença dos genes de resistência, a relação clonal, o tempo de morte em isolados de P. aeruginosa provenientes de Infecções relacionadas à saúde (IrAS) de hospitais públicos do Recife-PE, em 2014, e verificar in vitro a taxa de fagocitose e a produção de oxido nítrico (NO) por monócitos humanos quando infectados por P. aeruginosa tratada com IgG humana em associação a Meropenem e Polimixina E. Foram avaliados 32 isolados para a detecção de genes de resistência por PCR, e para a avaliação de similaridade genética por ERIC-PCR. Destes, foram selecionados os 5 isolados apresentando maior número de genes de resistência e menor similaridade genética. Esses cinco isolados foram submetidos às associações dos sub-CIMs destes antibióticos com a IgG humana, para a determinação do tempo de morte, da taxa de fagocitose de células bacterianas e para a dosagem de NO por monócitos humanos. Foi detectado a presença do gene blaKPC em dois isolados de P. aeruginosa. Todos os isolados possuem os genes mexR, mexB, mexE e rpsL, apenas um isolado foi negativo para os genes mexA e mexF. Foram detectados 30 perfis genéticos distintos entre os isolados bacterianos. O tempo de morte dos isolados revelou que os tratamentos combinados com antibióticos, principalmente Polimixina E, são mais letais para as células bacterianas. A taxa de fagocitose por monócitos humanos revelou que quando infectados com bactéria tratada com IgG mais antibiótico, os monócitos ficam mais ativos à fagocitose e reduz drasticamente a quantidade de células bacterianas. Houve diferença significativa na produção de NO naqueles tratados com Meropenem e IgG. Com o presente estudo concluiu-se que a combinação de IgG mais antibiótico pode ser uma alternativa para o tratamento dessas infecções, pois a bactéria estará em número reduzido facilitando a fagocitose. Advisors/Committee Members: SANTOS, Fábio André Bryner dos (advisor).

Subjects/Keywords: Pseudomonas aeruginosa; IgG; Antibióticos; Infecções

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APA (6^th Edition):

LIMA, F. C. G. d. (2015). Análise da ação do meropenem e Polimixina E com a IgG humana frente isolados de Pseudomonas aeruginosa provenientes de infecções relacionadas à Assistência à Saúde . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/14079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

LIMA, Fernanda Cristina Gomes de. “Análise da ação do meropenem e Polimixina E com a IgG humana frente isolados de Pseudomonas aeruginosa provenientes de infecções relacionadas à Assistência à Saúde .” 2015. Thesis, Universidade Federal de Pernambuco. Accessed July 17, 2019. http://repositorio.ufpe.br/handle/123456789/14079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

LIMA, Fernanda Cristina Gomes de. “Análise da ação do meropenem e Polimixina E com a IgG humana frente isolados de Pseudomonas aeruginosa provenientes de infecções relacionadas à Assistência à Saúde .” 2015. Web. 17 Jul 2019.

Vancouver:

LIMA FCGd. Análise da ação do meropenem e Polimixina E com a IgG humana frente isolados de Pseudomonas aeruginosa provenientes de infecções relacionadas à Assistência à Saúde . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2015. [cited 2019 Jul 17]. Available from: http://repositorio.ufpe.br/handle/123456789/14079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIMA FCGd. Análise da ação do meropenem e Polimixina E com a IgG humana frente isolados de Pseudomonas aeruginosa provenientes de infecções relacionadas à Assistência à Saúde . [Thesis]. Universidade Federal de Pernambuco; 2015. Available from: http://repositorio.ufpe.br/handle/123456789/14079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

3. Hodge, Lauren B. Effect of omega-3 fatty acid supplementation to gestating and lactating mares on milk IgG and fatty acid composition, mare and foal blood concentrations of IgG, fatty acid composition, insulin and glucose, and placental efficiency.

Degree: MS, Animal and Dairy Sciences, 2015, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06262015-110031/ ;

► There are conflicting results from previous research evaluating the effects of dietary omega-3 fatty acid supplementation on IgG concentration of colostrum, milk and foal… (more)

Subjects/Keywords: nutrition; Equine; IgG; omega-3

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APA (6^th Edition):

Hodge, L. B. (2015). Effect of omega-3 fatty acid supplementation to gestating and lactating mares on milk IgG and fatty acid composition, mare and foal blood concentrations of IgG, fatty acid composition, insulin and glucose, and placental efficiency. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-06262015-110031/ ;

Chicago Manual of Style (16^th Edition):

Hodge, Lauren B. “Effect of omega-3 fatty acid supplementation to gestating and lactating mares on milk IgG and fatty acid composition, mare and foal blood concentrations of IgG, fatty acid composition, insulin and glucose, and placental efficiency.” 2015. Masters Thesis, Mississippi State University. Accessed July 17, 2019. http://sun.library.msstate.edu/ETD-db/theses/available/etd-06262015-110031/ ;.

MLA Handbook (7^th Edition):

Hodge, Lauren B. “Effect of omega-3 fatty acid supplementation to gestating and lactating mares on milk IgG and fatty acid composition, mare and foal blood concentrations of IgG, fatty acid composition, insulin and glucose, and placental efficiency.” 2015. Web. 17 Jul 2019.

Vancouver:

Hodge LB. Effect of omega-3 fatty acid supplementation to gestating and lactating mares on milk IgG and fatty acid composition, mare and foal blood concentrations of IgG, fatty acid composition, insulin and glucose, and placental efficiency. [Internet] [Masters thesis]. Mississippi State University; 2015. [cited 2019 Jul 17]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06262015-110031/ ;.

Council of Science Editors:

Hodge LB. Effect of omega-3 fatty acid supplementation to gestating and lactating mares on milk IgG and fatty acid composition, mare and foal blood concentrations of IgG, fatty acid composition, insulin and glucose, and placental efficiency. [Masters Thesis]. Mississippi State University; 2015. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06262015-110031/ ;

Universidade Estadual de Campinas

4. Silva, Luana Cristina Andrade da, 1984-. Purificação de fragmentos Fab a partir de solução de clivagem enzimática de IgG humana : cromatografia de pseudoafinidade com quelatos metálicos e fenil boronato como ligantes .

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/266062

► Resumo: As imunoglobulinas do tipo G humana (IgG) são amplamente empregadas em tratamentos terapêuticos contra câncer, doenças auto-imunes e imunodeficiência adquirida e na área analítica,… (more)

▼ Resumo: As imunoglobulinas do tipo G humana (IgG) são amplamente empregadas em tratamentos terapêuticos contra câncer, doenças auto-imunes e imunodeficiência adquirida e na área analítica, em testes diagnósticos. Os fragmentos Fab podem ser obtidos por clivagem enzimática da molécula de IgG nativa ou por tecnologia de DNA recombinante. O fragmento Fab é composto por regiões constantes e variáveis, sendo que as regiões variáveis de cadeias leve e pesada compõem o fragmento Fv. Tanto os fragmentos Fab quanto os fragmentos Fv foram citados como segunda e terceira geração de anticorpos, respectivamente, e têm sido crescente o interesse em empregá-los em tratamentos terapêuticos, visto que ambos possuem menor massa molar em relação a IgG intacta e preservam a capacidade de se ligar ao antígeno. Como a utilização destas proteínas nas áreas terapêutica e analítica requer um alto grau de pureza, estratégias de purificação vêm sendo desenvolvidas. No intuito de contribuir para o desenvolvimento de processos de purificação de fragmento Fab, estudou-se o emprego de ligantes alternativos aos bioespecíficos proteínas A, G e L, comumente empregados para este fim. Neste trabalho, a purificação dos fragmentos Fab foi realizada por cromatografia de afinidade com íons metálicos imobilizados (IMAC) e fenil boronato (FB). Quanto a IMAC, avaliou-se o efeito dos agentes quelantes IDA (ácido iminodiacético) e TREN (Tris 2(aminoetil) amina), os íons metálicos Cu2+, Ni2+ e Co2+ e os tampões Tris-HCl, fosfato de sódio e HEPES na ausência e na presença de NaCl para a purificação de Fab a partir de solução de clivagem enzimática de IgG humana policlonal. Os resultados foram analisados conjuntamente por Western blot e imunodifusão radial. No que se refere à pureza dos fragmentos Fab obtidos na etapa de flowthrough, a ordem crescente obtida foi TREN-Ni2+ (100%) > IDA-Ni2 (88,6%) > IDA-Co2+ (71,1%) > IDA-Cu2+ (63,9%). Em relação ao rendimento, obteve-se a seguinte ordem: TREN-Ni2+ (86,2%) = IDA-Co2+ (86,2%) > IDA-Ni2+ (74,3%) = IDA-Cu2+ (73,7%). No tocante à capacidade de adsorção de proteína total por mL de gel, obteve-se a ordem 14,3 mg mL-1 ( TREN-Ni2+) > 11,2 mg mL-1 (IDA-Ni2+) > 5,65 mg mL-1 (IDA-Cu2+) > 4,3 mg mL-1 (IDA-Co2+). O ligante FB adsorveu os fragmentos Fab e Fc e não promoveu a separação destes. Este trabalho possibilitou concluir que os agentes quelantes IDA e TREN com os íons metálicos empregados, possibilitam separar os fragmentos Fab de fragmentos Fc e obtê-los com grau de pureza satisfatório; Abstract: Polyclonal human G immunoglobulins are widely applied in therapeutic treatments for cancer, autoimmune diseases and immunodeficiency and in the analytical area of diagnostic testing. The human IgG Fab fragments can be obtained via enzymatic cleavage of the native molecule or recombinant DNA technology. The Fab is composed of constant regions (CL and CH) and variable (VL and VH). The variable regions of the light and heavy chains comprise the Fv fragment. Fab fragments and Fv fragments were cited as second and third generation of… Advisors/Committee Members: Bueno, Sônia Maria Alves, 1961- (advisor), Bueno, Sonia Maria Alves (advisor), Azzoni, Adriano Rodrigues (committee member), Bresolin, Igor Tadeu Lazzaroto (committee member), Simioni, Patricia Ucelli (committee member), Tamashiro, Wirla Maria da Silva Cunha (committee member).

Subjects/Keywords: IgG; Purificação; Quelatos; Fenil

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APA (6^th Edition):

Silva, Luana Cristina Andrade da, 1. (2014). Purificação de fragmentos Fab a partir de solução de clivagem enzimática de IgG humana : cromatografia de pseudoafinidade com quelatos metálicos e fenil boronato como ligantes . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/266062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Luana Cristina Andrade da, 1984-. “Purificação de fragmentos Fab a partir de solução de clivagem enzimática de IgG humana : cromatografia de pseudoafinidade com quelatos metálicos e fenil boronato como ligantes .” 2014. Thesis, Universidade Estadual de Campinas. Accessed July 17, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/266062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Luana Cristina Andrade da, 1984-. “Purificação de fragmentos Fab a partir de solução de clivagem enzimática de IgG humana : cromatografia de pseudoafinidade com quelatos metálicos e fenil boronato como ligantes .” 2014. Web. 17 Jul 2019.

Vancouver:

Silva, Luana Cristina Andrade da 1. Purificação de fragmentos Fab a partir de solução de clivagem enzimática de IgG humana : cromatografia de pseudoafinidade com quelatos metálicos e fenil boronato como ligantes . [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2019 Jul 17]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/266062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva, Luana Cristina Andrade da 1. Purificação de fragmentos Fab a partir de solução de clivagem enzimática de IgG humana : cromatografia de pseudoafinidade com quelatos metálicos e fenil boronato como ligantes . [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/266062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Beutier, Héloïse. Plaquettes et neutrophiles : acteurs clés dans le choc allergique dépendant des IgG : Platelets and neutrophils : key players in IgG-induced anaphylaxis.

Degree: Docteur es, Immunologie, 2016, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2016PA066519

►

Le choc anaphylactique est une réaction allergique systémique qui survient en quelques minutes et pouvant être fatale. Mon travail de thèse s’articule autour de deux… (more)

▼

Le choc anaphylactique est une réaction allergique systémique qui survient en quelques minutes et pouvant être fatale. Mon travail de thèse s’articule autour de deux projets dont la finalité est de mieux comprendre le mécanisme physiopathologique. La première partie de ce travail consiste à étudier in vivo chez la souris les contributions des récepteurs Fc aux IgG (FcγRs), des cellules effectrices et des médiateurs contribuant dans un modèle d’anaphylaxie systémique passive induit par une sous-classe particulière d’IgG : des IgG1, des IgG2a ou des IgG2b monoclonales dirigées contre un même antigène. Cette étude a permis de démontrer que le FcγRIII, les neutrophiles et les monocytes/macrophages sont les acteurs majoritaires quelque soit la sous-classe d’IgG de souris ; en revanche, la participation des basophiles ainsi que la contribution relative des médiateurs histamine et PAF sont dépendantes de la sous-classe d’IgG utilisée. La deuxième partie de ce travail consiste à étudier plus particulièrement la population plaquettaire dans un modèle de souris humanisées. Contrairement à la souris, les plaquettes humaines expriment un FcγR, le FcγRIIA déjà identifié comme acteur clé de l’anaphylaxie. Un modèle de choc allergique induit par des IgG humaines dans des souris transgéniques pour le FcγRIIA m’a permis de tester l’hypothèse suivant laquelle les plaquettes participent à l’initiation et/ou à la propagation de la réaction. Ce modèle a permis de mettre en évidence une thrombocytopénie sévère, des complexes plaquettes-leucocytes circulants et de montrer que le transfert de plaquettes ou de leur surnageant restaure les signes cliniques du choc allergique.

Anaphylaxis is a systemic hyperacute allergic reaction that occurs within minutes and can be fatal. The aim of my PhD project is to investigate the physiopathological mechanisms underlying anaphylaxis induction. The first part of my work focused on the contribution of FcγRs, effector cells and mediators in passive murine models of systemic anaphylaxis induced by the different subclasses of mouse specific IgG ; directed against the same antigen: IgG1, IgG2a or IgG2b. This study demonstrated that FcγRIII, neutrophils and monocytes/macrophages are the key players of anaphylaxis induction whatever the mouse IgG subclasses used. On the contrary, basophil participation and the relative contribution of histamine and PAF are IgG subclass dependent. The second part of this work examined the role of platelets in anaphylaxis using a humanized mouse model. Opposing the murine situation, human platelets express an IgG receptor, FcγRIIA. This receptor has already been identified as a key player in anaphylaxis. Using aggregated human IgG to induce anaphylaxis in mice transgenic for FcγRIIA, we tested our hypothesis that platelets contribute to the initiation and/or the propagation of this reaction. Anaphylaxis in this model was accompanied by a severe thrombocytopenia, the presence of circulating platelet-leukocyte complexes and activated platelets. I further demonstrated that the…

Advisors/Committee Members: Bruhns, Pierre (thesis director), Jönsson, Friederike (thesis director).

Subjects/Keywords: Anaphylaxie; Récepteurs Fc aux IgG; Plaquettes; Neutrophiles; IgG; Platelet-Activating factor (PAF); Anaphylaxis; IgG receptors; Neutrophils; 571.96

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Beutier, H. (2016). Plaquettes et neutrophiles : acteurs clés dans le choc allergique dépendant des IgG : Platelets and neutrophils : key players in IgG-induced anaphylaxis. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066519

Chicago Manual of Style (16^th Edition):

Beutier, Héloïse. “Plaquettes et neutrophiles : acteurs clés dans le choc allergique dépendant des IgG : Platelets and neutrophils : key players in IgG-induced anaphylaxis.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed July 17, 2019. http://www.theses.fr/2016PA066519.

MLA Handbook (7^th Edition):

Beutier, Héloïse. “Plaquettes et neutrophiles : acteurs clés dans le choc allergique dépendant des IgG : Platelets and neutrophils : key players in IgG-induced anaphylaxis.” 2016. Web. 17 Jul 2019.

Vancouver:

Beutier H. Plaquettes et neutrophiles : acteurs clés dans le choc allergique dépendant des IgG : Platelets and neutrophils : key players in IgG-induced anaphylaxis. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2019 Jul 17]. Available from: http://www.theses.fr/2016PA066519.

Council of Science Editors:

Beutier H. Plaquettes et neutrophiles : acteurs clés dans le choc allergique dépendant des IgG : Platelets and neutrophils : key players in IgG-induced anaphylaxis. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066519

Universidad Nacional de La Plata

6. Garro, Adriana del Carmen. Estudio de la respuesta inmune humoral en la gestación porcina.

Degree: 2015, Universidad Nacional de La Plata

URL: http://hdl.handle.net/10915/54668

► La preñez en los mamíferos se atribuye a la regulación del sistema inmune materno por factores placentarios. En el presente trabajo de tesis se estudió… (more)

▼ La preñez en los mamíferos se atribuye a la regulación del sistema inmune materno por factores placentarios. En el presente trabajo de tesis se estudió la respuesta inmune humoral durante la gestación porcina. Se procesaron 45 tractos reproductivos y se recogieron sueros, placentas y muestras de tejidos placentarios maternos y fetales de 30 días (n=17); 65-70 días (n=9) y de 96-114 (n=6) días de preñez, así como de útero no gestante (n=13). Se estudió la presencia de IgG y su receptor en tejidos placentarios por inmunohistoquímica, se determinó la concentración de Ac IgG asimétricos mediante ELISA de captura y la concentración de IgG en suero y en extractos placentarios porcinos por inmunoturbidimetría e inmunodifusión radial. Se detectó la presencia de IgG y su receptor en tejidos placentarios maternos y fetales a partir de los 30 días de gestación hasta el final de la preñez. En placenta materna se halló positividad a IgG en todos los períodos estudiados, así como en los constituyentes de la placenta fetal, particularmente en las vellosidades que conforman la interfase feto materna. Se observó expresión positiva del receptor Fc gama de las IgG durante toda la preñez, salvo en glándulas uterinas al final de la gestación. En suero no encontramos diferencias significativas en el porcentaje de Ac IgG asimétricos entre cerdas vacías y preñadas, pero sí entre los diferentes períodos gestacionales, hallando disminución de Ac IgG asimétricos a los 30 días de preñez y aumento a los 95-114 días (32±3 vs 43±3, p: 0,01). En homogenatos de placenta materna se observó la mayor concentración de anticuerpos asimétricos a los 65-70 días (x=44,62), descendiendo los valores al final de la gestación (x=13,50), mientras que en homogenatos fetales las concentraciones de Ac IgG asimétricos permanecieron constantes durante toda la preñez (x=43,54). Se encontraron diferencias significativas en suero entre cerdas vacías y preñadas en la concentración de IgG/Turb (F: 4,17 y p: 0,0483), con una disminución al final de la preñez; mientras que los valores de IgG/IDR (F: 5,16 y p: 0,0316) fueron mayores a los hallados por turbidimetría (p: 0,1278; R2: 0,22), disminuyendo también al final de la preñez. En conclusión, se halló IgG y su receptor Fc gama sobre cortes histológicos placentarios, tanto maternos como fetales, a partir de los 30 días de preñez, se postula que las IgG serían de origen materno y que por transcitosis alcanzan la interfase feto materna. Se determinó la concentración de Ac IgG asimétricos durante la gestación porcina, no hallándose en suero diferencias significativas entre cerdas vacías y preñadas, probablemente debido al tipo de placenta epiteliocorial y no invasiva que presenta esta especie. Únicamente en los homogenatos de placenta fetal se hallaron valores altos de Ac IgG asimétricos a concentraciones constantes durante la preñez. Se postula que la respuesta inmune humoral que se instala durante la gestación, a través de los Ac IgG asimétricos, protegería al embrión/feto del sistema inmune materno posibilitando una… Advisors/Committee Members: Koncurat, Mirta Adriana, Mórtola, Eduardo Carlos, Portiansky, Enrique Leo, Sota, Rodolfo Luzbel de la.

Subjects/Keywords: Ciencias Veterinarias; Mamíferos; Porcinos; Reproducción; cerdo; placenta; IgG; Ac IgG asimétricos; receptor Fc-gama de IgG

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Garro, A. d. C. (2015). Estudio de la respuesta inmune humoral en la gestación porcina. (Thesis). Universidad Nacional de La Plata. Retrieved from http://hdl.handle.net/10915/54668

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Garro, Adriana del Carmen. “Estudio de la respuesta inmune humoral en la gestación porcina.” 2015. Thesis, Universidad Nacional de La Plata. Accessed July 17, 2019. http://hdl.handle.net/10915/54668.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Garro, Adriana del Carmen. “Estudio de la respuesta inmune humoral en la gestación porcina.” 2015. Web. 17 Jul 2019.

Vancouver:

Garro AdC. Estudio de la respuesta inmune humoral en la gestación porcina. [Internet] [Thesis]. Universidad Nacional de La Plata; 2015. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/10915/54668.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Garro AdC. Estudio de la respuesta inmune humoral en la gestación porcina. [Thesis]. Universidad Nacional de La Plata; 2015. Available from: http://hdl.handle.net/10915/54668

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Zambia

7. Farah, Ali Noor Abucar. Seroprevalence of anti-toxoplasma IgG antibody in HIV patients with neurological manifestations .

Degree: 2012, University of Zambia

URL: http://hdl.handle.net/123456789/1052

► Neurological complications of HIV disease secondary to opportunistic infections are significantly common. At least one third of patients with advanced HIV disease will present with… (more)

▼ Neurological complications of HIV disease secondary to opportunistic infections are significantly common. At least one third of patients with advanced HIV disease will present with neurological complications possibly secondary to opportunistic Infections such as Cerebral Toxoplasmosis. In patients with the acquired immunodeficiency syndrome (AIDS), toxoplasmic encephalitis (TE) is usually a presumptive diagnosis based on positive anti-toxoplasma Antibody (Ab), clinical manifestations and characteristic neuroradiological abnormalities. One hundred and nineteen patients were recruited in to this study; all of the participants were sero-positive for HIV, with a history suggestive of opportunistic infections of CNS or obvious neurological symptoms and signs such as headache and confusional state, meningeal irritation, cranial nerve deficits, adult onset seizures and hemiplegia. The aim of the study was to determine seroprevalence of anti-toxoplasma IgG Ab in HIV/AIDS patient with neurological manifestations, and to establish a rational means for the clinician facing decision making concerning empiric anti-toxoplasma therapy. The Study was conducted between November 2008 and January 2009 at the University Teaching Hospital, a major teaching hospital in Lusaka, Zambia, that still offers out-patient as well as inpatient health care to a wide spectrum of population. The study looked at male and female HIV positive adults regardless of whether or not they were on treatment with Highly Active Anti-retroviral therapy (HAART), who presented with central nervous system manifestations/ abnormalities. After obtaining informed consent from the participant or next of kin, history was obtained including demographic factors and anti-retroviral therapy state. Clinical examination was conducted for every participant with special emphasis on neurological examination. Blood samples were collected for serology test for Anti-toxoplasma IgG Ab using TOXO Direct Latex which is rapid slide agglutination procedure, developed for the direct detection of Anti-toxoplasma Ab in human serum. v The seroprevalence of toxoplasmosis was 11.8%, total number of specimen tested were 119 with frequency of 105 negative (88.2%) and 14 positive (11.8%). The majority of the patients were males 61(52.6%), the total number of female participants was 55 (47.4%). Most of the patients 94 (79.0%) were HAART naïve/pre-HAART, and 25 (21.0%) were on HAART. Common clinical manifestations of the patients enrolled were headache, neck-ache/stiffness, confusion, convulsion, fever and focal neurological lesions (hemi-plegia & cranial nerve deficit). Seroprevalence of toxoplasma was quite low as compared to other studies though this could be explained by the small sample size. Further investigations were warranted to determine the diagnostic ability of utilizing the toxoplasma Ab test

Subjects/Keywords: Anti-toxoplasma IgG – Antibody – HIV Patients

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APA (6^th Edition):

Farah, A. N. A. (2012). Seroprevalence of anti-toxoplasma IgG antibody in HIV patients with neurological manifestations . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/1052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Farah, Ali Noor Abucar. “Seroprevalence of anti-toxoplasma IgG antibody in HIV patients with neurological manifestations .” 2012. Thesis, University of Zambia. Accessed July 17, 2019. http://hdl.handle.net/123456789/1052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Farah, Ali Noor Abucar. “Seroprevalence of anti-toxoplasma IgG antibody in HIV patients with neurological manifestations .” 2012. Web. 17 Jul 2019.

Vancouver:

Farah ANA. Seroprevalence of anti-toxoplasma IgG antibody in HIV patients with neurological manifestations . [Internet] [Thesis]. University of Zambia; 2012. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/123456789/1052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Farah ANA. Seroprevalence of anti-toxoplasma IgG antibody in HIV patients with neurological manifestations . [Thesis]. University of Zambia; 2012. Available from: http://hdl.handle.net/123456789/1052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

8. Sitters, Sofie. Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/30490

► A commercial turbidimetric immunoassay (ARS Foal-IgG Test; Animal Reproduction Systems, Chino, CA), designed for measuring IgG concentrations in foal serum, was evaluated as a means… (more)

Subjects/Keywords: Diergeneeskunde; IgG; mare colostrum; refractometer; turbidimetric immunoassay

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APA (6^th Edition):

Sitters, S. (2008). Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/30490

Chicago Manual of Style (16^th Edition):

Sitters, Sofie. “Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed July 17, 2019. http://dspace.library.uu.nl:8080/handle/1874/30490.

MLA Handbook (7^th Edition):

Sitters, Sofie. “Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum.” 2008. Web. 17 Jul 2019.

Vancouver:

Sitters S. Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2019 Jul 17]. Available from: http://dspace.library.uu.nl:8080/handle/1874/30490.

Council of Science Editors:

Sitters S. Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/30490

Universiteit Utrecht

9. Zandstra, G.J. Immunoglobulin G determination for foals in the veterinary practice, which tests are most accurate.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/291925

► Monitoring the success of passive transfer of immunoglobulins is important because foals with failure of passive transfer are at increased risk for the development of… (more)

Subjects/Keywords: IgG; Neonatal foals; Protein; Screeningtests; Turbidimetric immunoassay

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APA (6^th Edition):

Zandstra, G. J. (2014). Immunoglobulin G determination for foals in the veterinary practice, which tests are most accurate. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/291925

Chicago Manual of Style (16^th Edition):

Zandstra, G J. “Immunoglobulin G determination for foals in the veterinary practice, which tests are most accurate.” 2014. Masters Thesis, Universiteit Utrecht. Accessed July 17, 2019. http://dspace.library.uu.nl:8080/handle/1874/291925.

MLA Handbook (7^th Edition):

Zandstra, G J. “Immunoglobulin G determination for foals in the veterinary practice, which tests are most accurate.” 2014. Web. 17 Jul 2019.

Vancouver:

Zandstra GJ. Immunoglobulin G determination for foals in the veterinary practice, which tests are most accurate. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2019 Jul 17]. Available from: http://dspace.library.uu.nl:8080/handle/1874/291925.

Council of Science Editors:

Zandstra GJ. Immunoglobulin G determination for foals in the veterinary practice, which tests are most accurate. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/291925

10. Pereira de Oliveira, Andresa. Aplicação da citometria de fluxo no diagnóstico da leishmaniose tegumentar americana .

Degree: 2011, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/9121

► A LTA é uma infecção parasitária tendo Leishmania (Viannia) braziliensis) como agente etiológico de maior incidência no Brasil. Em todas as formas clínicas da doença,… (more)

▼ A LTA é uma infecção parasitária tendo Leishmania (Viannia) braziliensis) como agente etiológico de maior incidência no Brasil. Em todas as formas clínicas da doença, sabe-se que a resposta imune é dependente de células T. Embora estudos avaliem a resposta humoral na LTA, ainda não está esclarecido o papel de anticorpos específicos na imunidade contra Leishmania. Além disso, o diagnóstico da LTA demonstra dificuldades pois o mesmo é realizado por associações clínicas, epidemiológicas e laboratoriais. Considerando os benefícios da citometria de fluxo, uma nova abordagem utilizada na pesquisa de anticorpos com aplicabilidade superior aos diferentes protocolos de detecção e revelação convencionais, este estudo trouxe uma alternativa através da pesquisa de anticorpos IgG de L(V)braziliensis, aplicável ao diagnóstico da LTA e critério de cura.O objetivo deste estudo foi comparar as técnicas de reação de imunofluorescência indireta (IFI) e citometria de fluxo na avaliação clínica-laboratorial dos pacientes antes e após cura clínica e avaliar a aplicabilidade da citometria de fluxo no monitoramento pós-terapêutico dos pacientes. Soros de 14 pacientes antes do tratamento (AT), 13 pacientes 1 ano após tratamento (PT), 10 pacientes 2 e 5 anos PT foram analisados e os resultados foram expressos em níveis de reatividade de IgG, a partir da porcentagem de parasitos fluorescentes positivos (PPFP). A diluição 1:256 das amostras permitiu diferenciar os indivíduos AT e PT. A análise comparativa da IFI e da citometria de fluxo pela curva ROC (Receiver Operating Characteristic Curve) demonstrou, respectivamente, ASC (área sob a curva) =0,78 (IC95%= 0,64-0,89) e ASC=0,90 (IC95%= 0,75-0.95), demonstrando elevada acurácia do teste de citometria de fluxo. Nossos dados demonstram que 20% foi o melhor ponto de corte identificado pela curva ROC para o ensaio de citometria de fluxo. Este ensaio apresentou sensibilidade de 86% e especificidade de 77%, enquanto a IFI apresentou sensibilidade de 71% e especificidade de 69%. O monitoramento pós terapêutico através da análise comparativa dos índices de desempenho das técnicas em 1, 2 e 5 anos PT, revelou melhor desempenho da citometria de fuxo em relação a IFI. Portanto, a citometria de fluxo demonstrou ser uma alternativa diagnóstica aplicada ao estudo da LTA e que as informações obtidas nesse estudo abrem perspectivas para o monitoramento de cura pós- terapêutica da LTA Advisors/Committee Members: Rêgo Alves Pereira, Valéria (advisor).

Subjects/Keywords: LTA; IgG; Citometria de fluxo; IFI.

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APA (6^th Edition):

Pereira de Oliveira, A. (2011). Aplicação da citometria de fluxo no diagnóstico da leishmaniose tegumentar americana . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/9121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pereira de Oliveira, Andresa. “Aplicação da citometria de fluxo no diagnóstico da leishmaniose tegumentar americana .” 2011. Thesis, Universidade Federal de Pernambuco. Accessed July 17, 2019. http://repositorio.ufpe.br/handle/123456789/9121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pereira de Oliveira, Andresa. “Aplicação da citometria de fluxo no diagnóstico da leishmaniose tegumentar americana .” 2011. Web. 17 Jul 2019.

Vancouver:

Pereira de Oliveira A. Aplicação da citometria de fluxo no diagnóstico da leishmaniose tegumentar americana . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2011. [cited 2019 Jul 17]. Available from: http://repositorio.ufpe.br/handle/123456789/9121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pereira de Oliveira A. Aplicação da citometria de fluxo no diagnóstico da leishmaniose tegumentar americana . [Thesis]. Universidade Federal de Pernambuco; 2011. Available from: http://repositorio.ufpe.br/handle/123456789/9121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

11. Piche-Nicholas, Nicole Melissa. Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor.

Degree: 2014, Boston University

URL: http://hdl.handle.net/2144/14377

► A large body of data exists demonstrating the key role of FcRn in extending the half-life of therapeutic antibodies by rescuing them from lysosomal degradation.… (more)

▼ A large body of data exists demonstrating the key role of FcRn in extending the half-life of therapeutic antibodies by rescuing them from lysosomal degradation. This led to the widely accepted hypothesis that FcRn binding of an IgG via the CH2-CH3 interface of Fc correlates with IgG half-life. Several studies have demonstrated that in vivo half-life can be modified by changing the binding affinity of IgG to FcRn. These modifications were generated by mutating the coding sequence for the Fc region that resulted in enhanced or reduced FcRn binding at endosomal pH without enhancing binding at neutral pH. In contrast to this, we have observed that the half-lifes of IgG molecules that had showed no target-mediated disposition or off-target binding varies widely, even when they share identical Fc domains. This led us to hypothesize that domains of IgG molecules other than Fc could contribute to the modulation of FcRn binding and affect in vivo half-life. This hypothesis was strengthened by recent publications by other groups showing a correlation between antibody charge and the FcRn affinity and/or in vivo half-life. In this study we explored the role of IgG domains other than the FcRn binding domain in altering the affinity between IgG and FcRn and its relation to the in vivo half-life. Here we describe a surface plasmon resonance (SPR) based assay developed to examine the steady-state binding affinity (KD) of IgG molecules to FcRn. We systematically dissected the contributions of IgG variable domain regions in modulating the affinity between FcRn and IgG. Through analysis of a broad collection of therapeutic antibodies containing more than 100 unique IgG molecules against more than 25 different therapeutic targets we have demonstrated that variable domains and in particular CDRs significantly alter binding affinity to FcRn, by 10 to 80-fold, whereas heavy and light chain isotypes do not. Because CDRs modulate the affinity between IgG and FcRn in our in vitro studies, it is important to understand the role they play in modulation of IgG half-life in vivo as this would have far-reaching implications in the half-life optimization efforts of IgG therapeutics.

Subjects/Keywords: Pharmacology; FcRn; IgG; Neonatal Fc receptor

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APA (6^th Edition):

Piche-Nicholas, N. M. (2014). Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor. (Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/14377

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Piche-Nicholas, Nicole Melissa. “Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor.” 2014. Thesis, Boston University. Accessed July 17, 2019. http://hdl.handle.net/2144/14377.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Piche-Nicholas, Nicole Melissa. “Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor.” 2014. Web. 17 Jul 2019.

Vancouver:

Piche-Nicholas NM. Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor. [Internet] [Thesis]. Boston University; 2014. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/2144/14377.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Piche-Nicholas NM. Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor. [Thesis]. Boston University; 2014. Available from: http://hdl.handle.net/2144/14377

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

12. Chen, Junbo. Fine granular deposition of clonal immunoreactivity on podocyte cell bodies: a primary podocytopathy marker and potential clue to disease mechanism.

Degree: MS, Pathology, 2018, Boston University

URL: http://hdl.handle.net/2144/31223

► Minimal change disease (MCD) and primary (idiopathic) focal segmental glomerulosclerosis (1FSGS), referred to collectively as “primary podocytopathies”, are major causes of nephrotic syndrome in children… (more)

▼ Minimal change disease (MCD) and primary (idiopathic) focal segmental glomerulosclerosis (1FSGS), referred to collectively as “primary podocytopathies”, are major causes of nephrotic syndrome in children and adults, and are thought to be due to direct podocyte damage visible only at the electron microscopic level. Lupus podocytopathy (LP) is a newly recognized entity that involves severe podocyte injury in the setting of systemic lupus erythematosus, in the complete absence of peripheral capillary wall immune deposits. All of these pathologic diagnoses hinge on the ultrastructural finding of severe podocyte injury and foot process effacement. In addition to these ultrastructural changes, we have observed the presence of fine granular anti-IgG antibody immunoreactivity on podocyte cell bodies in kidney biopsies of patients with MCD, LP, and some patients with the tip lesion variant and NOS variants of 1FSGS. To validate this finding, we compared antibody staining from primary podocytopathy biopsies with those in biopsies from patients with other disease states, including lesions associated with severe podocyte injury in the absence of immune deposits: secondary (adaptive) focal segmental glomerulosclerosis, thin basement membrane disease, diabetic nephropathy, and renal amyloidosis. We found that a fine granular pattern of anti-IgG immunoreactivity on podocyte cell bodies is a specific morphologic feature of the primary podocytopathies, including virtually all cases of MCD that we encountered, some instances of tip lesion variant and NOS variant of 1FSGS, and one cases of LP. The antigen targeted by the anti-IgG immunostaining in these biopsies exhibited one of several oligoclonal IgG heavy chain subtype plus light chain profiles. Ultra-high resolution microscopy revealed fine linear anti-IgG staining along filtration slit diaphragms, suggesting that IgG deposition may potentially be targeting a filtration slit-associated antigen such as podocin. Our findings suggest the possibility of a direct antibody-mediated mechanism of podocyte injury in the primary podocytopathies, one that potentially targets podocyte-specific protein structures, and which may provide a specific and more rapid diagnostic marker for this group of diseases. The findings also suggest an etiologic relationship between MCD and some instances of 1FSGS. Advisors/Committee Members: Henderson, Joel M. (advisor), Andry, Christopher D. (advisor).

Subjects/Keywords: Pathology; IgG; Minimal change disease; Podocyte

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APA (6^th Edition):

Chen, J. (2018). Fine granular deposition of clonal immunoreactivity on podocyte cell bodies: a primary podocytopathy marker and potential clue to disease mechanism. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/31223

Chicago Manual of Style (16^th Edition):

Chen, Junbo. “Fine granular deposition of clonal immunoreactivity on podocyte cell bodies: a primary podocytopathy marker and potential clue to disease mechanism.” 2018. Masters Thesis, Boston University. Accessed July 17, 2019. http://hdl.handle.net/2144/31223.

MLA Handbook (7^th Edition):

Chen, Junbo. “Fine granular deposition of clonal immunoreactivity on podocyte cell bodies: a primary podocytopathy marker and potential clue to disease mechanism.” 2018. Web. 17 Jul 2019.

Vancouver:

Chen J. Fine granular deposition of clonal immunoreactivity on podocyte cell bodies: a primary podocytopathy marker and potential clue to disease mechanism. [Internet] [Masters thesis]. Boston University; 2018. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/2144/31223.

Council of Science Editors:

Chen J. Fine granular deposition of clonal immunoreactivity on podocyte cell bodies: a primary podocytopathy marker and potential clue to disease mechanism. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/31223

University of Texas – Austin

13. -8757-3869. Mass spectrometry combined with strategic enzymatic digestion, selective derivatization and ultraviolet photodissociation for the identification and characterization of Immunoglobulin G antibodies.

Degree: Chemistry, 2016, University of Texas – Austin

URL: http://hdl.handle.net/2152/72663

► Immunoglobulin G (IgG) antibodies represent important analytical targets both for their therapeutic properties and for their critical role in the adaptive immune response. While much… (more)

▼ Immunoglobulin G (IgG) antibodies represent important analytical targets both for their therapeutic properties and for their critical role in the adaptive immune response. While much of the primary structure is conserved across the IgG class, subtle changes in amino acid sequence and the presence or absence of post-translational modifications can have a profound effect on the function and therapeutic potential of a given antibody. As such, there remains a high demand for versatile analytical tools capable of both identification and complete structural characterization of IgGs. The work presented in this dissertation largely focuses on the development of mass spectrometry-based methods for the improved analysis of antibodies. This was accomplished using strategic enzymatic Brodbeltselectivity for regions of particular diagnostic value or to facilitate comprehensive structural characterization. A method based on chromophore-mediated 351 nm UVPD was developed as a means to streamline the identification of antibodies in mixtures by enhancing selectively for the third complementarity determining region of the IgG heavy chain (CDR-H3). The hypervariable sequences within this region serve as the primary determinant of antigen binding specificity and thus provide a molecular signature by which to differentiate unique antibodies. To accomplish this, a highly conserved cysteine residue located in the framework preceding the CDR-H3 region was exploited for selective tagging with an Alexa Fluor 350 (AF350) thiol-selective maleimide. This site-specific tagging combined with strategic enzymatic digestion and 351 nm UVPD allowed selective dissociation of only AF350-labeled peptides for facile discrimination of CDR-H3 sequences within a high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) based workflow. Two variations of middle-down mass spectrometry based on either restricted Lys-C proteolysis or hinge-selective IdeS digestion combined with 193 nm UVPD were used for the characterization of monoclonal antibodies. Both strategies yielded considerably greater diagnostic sequence information when benchmarked against conventional collision- and electron-based activation methods. The Lys-C proteolysis method was found to have considerable implications for the analysis of serological antibody repertoires owing to its facile implementation into high-throughput proteomic workflows and ability to unambiguously differentiate unique CDR-H3 sequences. The development and implementation of a front-end dual spray reactor for high-throughput ion/ion-mediated bioconjugation is demonstrated for the enhanced structural characterization of unmodified and post-translationally modified peptide cations by 193 nm UVPD and CID. The ability to generate ion/ion complexes in real-time followed by efficient covalent conversion allowed integration of the dual spray reactor into a high-throughput LC-MS [superscript n] workflow for rapid derivatization of peptide mixtures. Advisors/Committee Members: Brodbelt, Jennifer S. (advisor), Crooks, Richard M (committee member), Georgiou, George (committee member), Webb, Lauren J (committee member), Zhang, Yan (committee member).

Subjects/Keywords: Mass spectrometry; Antibodies; IgG; Ultraviolet photodissociation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

-8757-3869. (2016). Mass spectrometry combined with strategic enzymatic digestion, selective derivatization and ultraviolet photodissociation for the identification and characterization of Immunoglobulin G antibodies. (Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/72663

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

-8757-3869. “Mass spectrometry combined with strategic enzymatic digestion, selective derivatization and ultraviolet photodissociation for the identification and characterization of Immunoglobulin G antibodies.” 2016. Thesis, University of Texas – Austin. Accessed July 17, 2019. http://hdl.handle.net/2152/72663.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

-8757-3869. “Mass spectrometry combined with strategic enzymatic digestion, selective derivatization and ultraviolet photodissociation for the identification and characterization of Immunoglobulin G antibodies.” 2016. Web. 17 Jul 2019.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-8757-3869. Mass spectrometry combined with strategic enzymatic digestion, selective derivatization and ultraviolet photodissociation for the identification and characterization of Immunoglobulin G antibodies. [Internet] [Thesis]. University of Texas – Austin; 2016. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/2152/72663.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

-8757-3869. Mass spectrometry combined with strategic enzymatic digestion, selective derivatization and ultraviolet photodissociation for the identification and characterization of Immunoglobulin G antibodies. [Thesis]. University of Texas – Austin; 2016. Available from: http://hdl.handle.net/2152/72663

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Kansas State University

14. Haukos, Kaitlin A. Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay.

Degree: MSin Biomedical Sciences, Department of Clinical Sciences, 2017, Kansas State University

URL: http://hdl.handle.net/2097/35556

► To protect horses from disease, equine practitioners typically prescribe a protocol of an initial primary vaccination followed by a booster vaccination 3-4 weeks later. Subsequent… (more)

▼ To protect horses from disease, equine practitioners typically prescribe a protocol of an initial primary vaccination followed by a booster vaccination 3-4 weeks later. Subsequent boosters are given every 6-12 months depending on the pathogen of concern. Each vaccination incurs an additional cost and increased chance for adverse reactions. Despite wide-spread protocol acceptance, duration of effectiveness of vaccines in protecting horses from disease is not well documented. It was hypothesized that horses vaccinated annually since birth have increased antibody production that remains consistent and sufficient for long-term protection from common diseases. This work resulted in the development of a novel, multiplex-magnetic bead-based indirect immunoassay to screen sera from vaccinated adult horses to measure antibody levels in response to vaccine administration. Antigens tested included West Nile Virus, Eastern Equine Encephalitis, Western Equine Encephalitis, Equine Influenza Virus, Equine Herpes Virus 1 and 4, Tetanus, and 7 different Rabies antigens (3 lab and 4 wild strains). The developed assay was a 7-plex capture antibody, which quantified equine IgG (Immunoglobulin G) that binds viral antigens derived from different rabies virus strains along with pure vaccine samples of the 7 different antigens. A 7-point standard curve was developed to quantify the viral-antigen reactive IgG concentration in vaccinated horse serum. Vaccinated horses increased serum antibody concentration for each antigen post-vaccination with the percent increase ranging between 34.0% for Equine Herpes Virus 4 and 257.3% for Equine Influenza Virus. Use of the novel assay will provide equine veterinarians with an economical method to measure immune activation toward common pathogens of concern. This methodology will provide foundation level information regarding antigen specific IgG concentrations that ultimately may be extrapolated to establish protective levels of immunity resulting in establishment of vaccine protocols. Advisors/Committee Members: Elizabeth G. Davis.

Subjects/Keywords: Equine; IgG; Vaccine reaction; Microsphere Assay; Serology

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APA (6^th Edition):

Haukos, K. A. (2017). Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay. (Masters Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/35556

Chicago Manual of Style (16^th Edition):

Haukos, Kaitlin A. “Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay.” 2017. Masters Thesis, Kansas State University. Accessed July 17, 2019. http://hdl.handle.net/2097/35556.

MLA Handbook (7^th Edition):

Haukos, Kaitlin A. “Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay.” 2017. Web. 17 Jul 2019.

Vancouver:

Haukos KA. Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay. [Internet] [Masters thesis]. Kansas State University; 2017. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/2097/35556.

Council of Science Editors:

Haukos KA. Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay. [Masters Thesis]. Kansas State University; 2017. Available from: http://hdl.handle.net/2097/35556

Universidade Estadual de Campinas

15. Suzuki, Lisandra Akemi. Resposta imune humoral na neurocisticercose .

Degree: 2010, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308741

► Resumo: A neurocisticercose (NC) e uma importante causa de doença neurológica em muitos paises em desenvolvimento, incluindo o Brasil. O diagnostico clinico da NC e… (more)

▼ Resumo: A neurocisticercose (NC) e uma importante causa de doença neurológica em muitos paises em desenvolvimento, incluindo o Brasil. O diagnostico clinico da NC e dificultado pelo polimorfismo e pela não especificidade dos sintomas. As tecnicas de neuroimagem e pesquisa de anticorpos específicos tem contribuído para o diagnostico da NC e uma melhor compreensão dos processos fisiopatológicos dessa infecção. O presente trabalho teve como objetivo avaliar, por meio de técnicas imunoenzimaticas (ELISA), a resposta imune humoral na NC, utilizando como preparações antigênicas o liquido vesicular (LV) e uma fração glicoproteica obtida do extrato bruto de cisticercos de Taenia solium (T. solium) com afinidade por lentil-lectina (fração Gp). Cinquenta e seis amostras de liquido cefalorraquidiano (LCR), 22 de pacientes com NC e 34 de pacientes com outros problemas neurológicos, foram utilizadas para a pesquisa de IgG e suas subclasses, com os seguintes resultados: IgG-LV: 100% de sensibilidade e especificidade; IgG1 -LV: 72,73% de sensibilidade e 100% de especificidade; IgG2-LV: 81,81% de sensibilidade e 100% de especificidade; IgG3-LV: 59,09% de sensibilidade e 97,06% de especificidade; IgG4-LV: 90,91% de sensibilidade e 97,06% de especificidade; IgG-fração Gp: 90,91% de sensibilidade e 97,06% de especificidade; IgG1-fração Gp: 59,09% de sensibilidade e 91,18% de especificidade; IgG2-fração Gp: 68,18% de sensibilidade e 94,12% de especificidade; IgG3-fração Gp: 36,36% de sensibilidade e 100% de especificidade; IgG4-fração Gp: 86,36% de sensibilidade e 100% de especificidade. Quarenta e sete amostras de LCR, 16 de pacientes com NC e 31 de pacientes com outros problemas neurológicos foram utilizadas para a pesquisa de IgE, com os seguintes resultados: IgE-LV e IgE-fração Gp: 93,75% de sensibilidade e 100% de especificidade. Cinquenta e sete amostras de soros, 22 de pacientes com NC, 18 de pacientes com outras infecções e 17 de pessoas presumivelmente sadias, foram utilizadas para a pesquisa da IgG e suas subclasses, IgE, IgA e IgM, com os seguintes resultados: IgG-LV: 100% de sensibilidade e especificidade; IgG1-LV: 86,36% de sensibilidade e 94,28% de especificidade; IgG2-LV: 90,91% de sensibilidade e 97,14% de especificidade; IgG3-LV: 86,36% de sensibilidade e 97,14% de especificidade; IgG4-LV: 100% de sensibilidade e de especificidade; IgG-fração Gp: 95,45% de sensibilidade e 100% de especificidade; IgG1-fração Gp: 63,64% de sensibilidade e 94,28% de especificidade; IgG2-fração Gp: 68,18% de sensibilidade e 97,14% de especificidade; IgG3-fração Gp: 54,54% de sensibilidade e 88,57% de especificidade; IgG4-fração Gp: 90,91% de sensibilidade e 100% de especificidade; IgELV: 90,91% de sensibilidade e 97,14% de especificidade; IgE-fração Gp: 86,36% de sensibilidade e 100% de especificidade; IgA-LV: 54,54% de sensibilidade e 94,28% de especificidade; IgA-fração Gp: 13,63% de sensibilidade e 100% de especificidade. Anticorpos IgM não foram detectados com as preparações de LV e fração Gp. Nossos resultados mostraram que, com ambas… Advisors/Committee Members: Rossi, Claudio Lucio, 1950- (advisor), Livramento, Jose Antonio (committee member), Gomes, Helio Rodrigues (committee member), Garlipp, Celia Regina (committee member), Mazon, Silvia de Barros (committee member).

Subjects/Keywords: Neurocisticercose; Imunoglobulinas; IgG; IgE; IgA; IgM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suzuki, L. A. (2010). Resposta imune humoral na neurocisticercose . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/308741

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suzuki, Lisandra Akemi. “Resposta imune humoral na neurocisticercose .” 2010. Thesis, Universidade Estadual de Campinas. Accessed July 17, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308741.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suzuki, Lisandra Akemi. “Resposta imune humoral na neurocisticercose .” 2010. Web. 17 Jul 2019.

Vancouver:

Suzuki LA. Resposta imune humoral na neurocisticercose . [Internet] [Thesis]. Universidade Estadual de Campinas; 2010. [cited 2019 Jul 17]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308741.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suzuki LA. Resposta imune humoral na neurocisticercose . [Thesis]. Universidade Estadual de Campinas; 2010. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308741

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lund

16. Sjögren, Jonathan. Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy.

Degree: 2015, University of Lund

URL: http://lup.lub.lu.se/record/5050759 ; http://portal.research.lu.se/ws/files/3040734/5050807.pdf

► A majority of the proteins of the immune system are glycosylated and the glycans of IgG are essential for its functionality. Bacteria display enzymes that… (more)

Subjects/Keywords: Infektionsmedicin; IgG; endoglycosidase; Streptococcus pyogenes; glycosylation; EndoS

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APA (6^th Edition):

Sjögren, J. (2015). Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy. (Doctoral Dissertation). University of Lund. Retrieved from http://lup.lub.lu.se/record/5050759 ; http://portal.research.lu.se/ws/files/3040734/5050807.pdf

Chicago Manual of Style (16^th Edition):

Sjögren, Jonathan. “Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy.” 2015. Doctoral Dissertation, University of Lund. Accessed July 17, 2019. http://lup.lub.lu.se/record/5050759 ; http://portal.research.lu.se/ws/files/3040734/5050807.pdf.

MLA Handbook (7^th Edition):

Sjögren, Jonathan. “Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy.” 2015. Web. 17 Jul 2019.

Vancouver:

Sjögren J. Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy. [Internet] [Doctoral dissertation]. University of Lund; 2015. [cited 2019 Jul 17]. Available from: http://lup.lub.lu.se/record/5050759 ; http://portal.research.lu.se/ws/files/3040734/5050807.pdf.

Council of Science Editors:

Sjögren J. Bacterial modulation of host glycosylation - in infection, biotechnology, and therapy. [Doctoral Dissertation]. University of Lund; 2015. Available from: http://lup.lub.lu.se/record/5050759 ; http://portal.research.lu.se/ws/files/3040734/5050807.pdf

17. Pinto, Juliano Ventura. Avaliação da presença do Fator XI de coagulação em preparações de imunoglobulina G para uso intravenoso.

Degree: Mestrado, Biotecnologia, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-10122014-145928/ ;

►

A disponibilidade de hemoderivados é um parâmetro importante para medir a qualidade da saúde em um país. Dentre os produtos hemoderivados, imunoglobulinas tem alto valor… (more)

▼

A disponibilidade de hemoderivados é um parâmetro importante para medir a qualidade da saúde em um país. Dentre os produtos hemoderivados, imunoglobulinas tem alto valor agregado. O Instituto Butantan tem por objetivo o estabelecimento de uma planta industrial para fracionamento de plasma, com um processo produtivo baseado principalmente em cromatografias. Eventos tromboembólicos a partir de infusões de imunoglobulinas por via intravenosa (IgIV) foram relacionados com presença de Fator XI de coagulação (FXI) como contaminantes nas preparações de IgIVs. Com objetivo de detectar o FXI nas frações, o processo cromatográfico foi testado em escala piloto, bancada, e em cromatografia direta e o FXI foi dosado nas frações iniciais e no produto final IgIV. Foram estabelecidos os métodos de dosagem de atividade de FXI por tempo de coagulação e ensaio cromogênico. Concluímos que o FXI acompanha a IgG nas etapas iniciais dos processos cromatográficos e verificamos que ocorre a presença de FXI nos produtos finais. Este trabalho contribui para o desenvolvimento do conjunto de testes de controle de qualidade de biofármacos derivados de plasma humano.

The availability of hemoderivatives is an important parameter to measure a countrys health quality. Among hemoderivatives products, immunoglobulin have high value.. Instituto Butantan, aims the establishment of an industrial plant for plasma fractionation with a process drawn, based mainly in chromatographies. Thromboembolic events from infusions of intravenous immunoglobulins (IVIg) have been related with the presence of coagulation Factor XI (FXI) as contaminant in the IVIG preparations. With an objective of tracking FXI in the chromatographic fractions, the process was tested in pilot and bench scales and in direct chromatography and the FXI was measured in the initial fractions and in the final product IVIg. The methods of measurement of FXI activity thru coagulation time and chromogenic assay were established. We have concluded that FXI accompanies IgG in the early stages of the chromatographic processes and verified that the presence occurs in the final products, This work contributes to the development of the set of quality control tests of biopharmaceuticals derivatives from human plasma.

Advisors/Committee Members: Martins, Elizabeth Angelica Leme.

Subjects/Keywords: aTTP; aTTP; Chromogenic; Cromogênico; Efeitos tromboembólicos; FXI; FXI; Hemoderivados; Hemoderivatives; IgG; IgG; Thromboembolic effects

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APA (6^th Edition):

Pinto, J. V. (2014). Avaliação da presença do Fator XI de coagulação em preparações de imunoglobulina G para uso intravenoso. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/87/87131/tde-10122014-145928/ ;

Chicago Manual of Style (16^th Edition):

Pinto, Juliano Ventura. “Avaliação da presença do Fator XI de coagulação em preparações de imunoglobulina G para uso intravenoso.” 2014. Masters Thesis, University of São Paulo. Accessed July 17, 2019. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-10122014-145928/ ;.

MLA Handbook (7^th Edition):

Pinto, Juliano Ventura. “Avaliação da presença do Fator XI de coagulação em preparações de imunoglobulina G para uso intravenoso.” 2014. Web. 17 Jul 2019.

Vancouver:

Pinto JV. Avaliação da presença do Fator XI de coagulação em preparações de imunoglobulina G para uso intravenoso. [Internet] [Masters thesis]. University of São Paulo; 2014. [cited 2019 Jul 17]. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-10122014-145928/ ;.

Council of Science Editors:

Pinto JV. Avaliação da presença do Fator XI de coagulação em preparações de imunoglobulina G para uso intravenoso. [Masters Thesis]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-10122014-145928/ ;

Addis Ababa University

18. Hassen, Mamo. Analysis of Humoral Immune Response to a Panel of Plasmodium falciparum Blood- Stage Vaccine Candidate Antigens in Naturally Primed Populations in Seasonal Malaria Settings in Ethiopia .

Degree: 2013, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/429

► In Ethiopia, the general population is quite vulnerable to unpredictable cyclic epidemics of Plasmodium falciparum malaria. However, there is very little information on the anti-malaria… (more)

▼ In Ethiopia, the general population is quite vulnerable to unpredictable cyclic epidemics of Plasmodium falciparum malaria. However, there is very little information on the anti-malaria immune profile of the population residing in the endemic regions of the country. This study was designed to investigate the nature of humoral immune response to malaria in two population groups in two endemic localities, Shewa Robit in the north and Boditi in south. In a cross-sectional study, the study participants were diagnosed for malaria infection microscopically and by the rapid diagnostic test (RDT). The sera were tested by using enzyme-linked immunosorbent assay (ELISA) for total immunoglobulin (Ig) G against P. falciparum blood-stage vaccine candidate antigens: apical membrane antigen 1 (AMA1), glutamate-rich protein (GLURP) R2 region, and merozoite surface protein 2 (MSP2) allelic variants (3D7 and FC27) in Shewa Robit. Total IgG against GLURP-R0, MSP3 and GMZ2 and IgG subclasses against GLURP-R0 and MSP3 were assayed in both Shewa Robit and Boditi sera. Whereas 23(8.6%) blood-smear-positive cases for P. falciparum were detected in Boditi, all Shewa Robit study participants had no detectable P. falciparum infection. At both localities total IgG prevalence and levels to GMZ2 were significantly higher than the response to the component domains iv (GLURP-R0 and MSP3) indicating the induction of strong GMZ2-specific natural antibodies. There was significant difference between the median antibody level to GMZ2, GLURP-R0 and MSP3 compared to the responses to other antigens tested in Shewa Robit, indicating that GMZ2 could be a more relevant blood-stage malaria vaccine candidate antigen. Higher total IgG and subclass prevalence and levels were detected in Shewa Robit than Boditi, suggesting difference in the intensity of malaria transmission in the two localities and/or genetic differences between the two population groups in their response to blood-stage P. falciparum antigens. In both study sites, IgG subclass antibody levels to GLURP-R0 were significantly higher than that to MSP3 for all corresponding subclasses in most individuals, indicating the higher relative immunogenicity and protective potential of GLURP-R0 compared to MSP3. Against both GLURP-R0 and MSP3, the ratio of cytophilic to noncytophilic antibodies was >1 in the majority of the study participants, in both study sites, indicating the induction of protective antibodies against the two antigens. Analysis of age-related pattern in antibody levels against the antigens tested showed a positive association with increasing age for most antigens suggesting the role of intrinsic agerelated factors in immune maturation. The age factor appears plausible as there was no evidence for increase in antibody response with increasing frequency of reported past clinical malaria. Overall, the study has shown that Ethiopian population groups residing in unstable and seasonal malaria epidemiological settings have a high prevalence and levels of long-lived antibodies that readily… Advisors/Committee Members: Prof. Beyene Petros (advisor).

Subjects/Keywords: Antigen; Blood-stage vaccine; Cytophilic IgG subclass; ELISA; Ethiopia; Falciparum malaria; Noncytophilic IgG subclass

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APA (6^th Edition):

Hassen, M. (2013). Analysis of Humoral Immune Response to a Panel of Plasmodium falciparum Blood- Stage Vaccine Candidate Antigens in Naturally Primed Populations in Seasonal Malaria Settings in Ethiopia . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hassen, Mamo. “Analysis of Humoral Immune Response to a Panel of Plasmodium falciparum Blood- Stage Vaccine Candidate Antigens in Naturally Primed Populations in Seasonal Malaria Settings in Ethiopia .” 2013. Thesis, Addis Ababa University. Accessed July 17, 2019. http://etd.aau.edu.et/dspace/handle/123456789/429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hassen, Mamo. “Analysis of Humoral Immune Response to a Panel of Plasmodium falciparum Blood- Stage Vaccine Candidate Antigens in Naturally Primed Populations in Seasonal Malaria Settings in Ethiopia .” 2013. Web. 17 Jul 2019.

Vancouver:

Hassen M. Analysis of Humoral Immune Response to a Panel of Plasmodium falciparum Blood- Stage Vaccine Candidate Antigens in Naturally Primed Populations in Seasonal Malaria Settings in Ethiopia . [Internet] [Thesis]. Addis Ababa University; 2013. [cited 2019 Jul 17]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hassen M. Analysis of Humoral Immune Response to a Panel of Plasmodium falciparum Blood- Stage Vaccine Candidate Antigens in Naturally Primed Populations in Seasonal Malaria Settings in Ethiopia . [Thesis]. Addis Ababa University; 2013. Available from: http://etd.aau.edu.et/dspace/handle/123456789/429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Harvard University

19. Mahan, Alison Emilia. Regulation and Programming of Antibody Effector Function through IgG Glycosylation.

Degree: PhD, Biology: Medical Sciences, Division of, 2014, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070040

► Antibodies are the defining characteristic of the humoral immune response. Their functions are diverse, including direct neutralization of pathogens and recruitment of other immune molecules… (more)

▼ Antibodies are the defining characteristic of the humoral immune response. Their functions are diverse, including direct neutralization of pathogens and recruitment of other immune molecules or cells. While most successful vaccines induce protective neutralizing antibody responses, effective vaccine-elicited neutralizing antibodies against some pathogens, including HIV, HCV, malaria, and TB, remain elusive. Thus, researchers have begun to focus on how vaccines can elicit strong non-neutralizing antibody functions, including recruitment of innate immune factors for antibody-dependent cellular cytotoxicity, complement deposition, and anti\-body-dependent phagocytosis. The antibody's constant region (Fc) mediates most effector functions through isotype and subclass selection or alteration of the structure of the Fc-attached N-glycan, which controls function with exquisite specificity. Glycan modifications are naturally induced during inflammatory conditions such as autoimmune disease and natural infection however, the specific signals that regulate Fc-glycosylation remain unknown. This dissertation sought to understand how antibody glycosylation is regulated and how it can be programmed through vaccination. To do this, we first developed a technique to analyze antibody glycan structures both of bulk Fc and antigen-specific antibodies. Using this technique, we observed significant modulation of antibody glycans during viral infection as well as in vaccine-elicited antibodies. To identify specific signals important for altering the antibody glycan, we transcriptionally profiled stimulated B cells and identified a set of innate and adaptive stimuli that regulate the genes responsible for antibody glycosylation. The results described in this dissertation begin to define the specific mechanism(s) by which infection and vaccination modulate antibody glycosylation to elicit functional antibodies that can ultimately provide effective and sustained protection from infection. Advisors/Committee Members: Alter, Galit (advisor), Ribbeck, Katharina (committee member), Anthony, Robert (committee member), Kagan, Jonathan (committee member).

Subjects/Keywords: Biology; Immunology; Virology; effector function; Fc function; glycosylation; HIV; IgG; IgG-glycan

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mahan, A. E. (2014). Regulation and Programming of Antibody Effector Function through IgG Glycosylation. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070040

Chicago Manual of Style (16^th Edition):

Mahan, Alison Emilia. “Regulation and Programming of Antibody Effector Function through IgG Glycosylation.” 2014. Doctoral Dissertation, Harvard University. Accessed July 17, 2019. http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070040.

MLA Handbook (7^th Edition):

Mahan, Alison Emilia. “Regulation and Programming of Antibody Effector Function through IgG Glycosylation.” 2014. Web. 17 Jul 2019.

Vancouver:

Mahan AE. Regulation and Programming of Antibody Effector Function through IgG Glycosylation. [Internet] [Doctoral dissertation]. Harvard University; 2014. [cited 2019 Jul 17]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070040.

Council of Science Editors:

Mahan AE. Regulation and Programming of Antibody Effector Function through IgG Glycosylation. [Doctoral Dissertation]. Harvard University; 2014. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070040

Universitat Autònoma de Barcelona

20. Mateu Pruñonosa, Lourdes. Caracterización clínica e inmunológica de la inmunodeficiencia común variable, la deficiencia de subclases de Inmunoglobulina G y la deficiencia de Inmunoglobulina A en adultos.

Degree: Departament de Medicina, 2017, Universitat Autònoma de Barcelona

URL: http://hdl.handle.net/10803/457985

► Predominant antibody deficiencies are the most frequent primary immunodeficiencies (PID) in adults. This is a very heterogeneous group of immunodeficiencies, that include patients with a… (more)

▼ Predominant antibody deficiencies are the most frequent primary immunodeficiencies (PID) in adults. This is a very heterogeneous group of immunodeficiencies, that include patients with a large clinical spectrum with no correlation with immunoglobulin concentrations. Since the 1990s different diagnostic criteria and classifications for PID have been proposed. PID is a pathology poorly known by most physicians. This, and the great clinical variability of the manifestations, lead both to a lack and to a delay in the diagnosis of PID. For a correct diagnosis of common variable immunodeficiency (CVI), deficiency of IgG subclasses and IgA deficiency, it is essential to perform a functional study in order to evaluate the specific antibody response to polysaccharide antigens. Lung disease in adult patients with PID is the leading cause of morbidity and mortality with an important deterioration of their quality of life. In the present study we described a cohort of 85 adult patients with PID monitored by the Infectious Diseases Unit of Germans Trias i Pujol Hospital. Most patients belong to the group of isotope or light chain deficiencies with generally normal numbers of B cells. They are diagnosed in the fifth decade of their life, with a slight predominance of women. Approximately one-third of the patients present chronic lung disease and bronchiectasis when diagnosed with PID; more than a third of the patients present a poor response to the vaccines and a quarter of the patients require replacement therapy with immunoglobulins. Clinical, radiological and laboratory characteristics of patients diagnosed with hypogammaglobulinemia of uncertain significance (HGUS) have been compared with those diagnosed with probable or possible CVI - according to the 2013 Ameratunga criteria -, and no differences between the two groups were found. Although patients with HGUS had a conservative management, they did not present a worse prognosis. We also compared clinical, analytical and radiological characteristics of patients with PID, dividing those who were diagnosed before 2009, and those who were diagnosed after. From 2009 to present, we have performed different interventions in order to improve the awareness of these diseases among physicians of our area. We observed an increase in the PID diagnosis and a tendency to reduce the diagnosis delay. No differences were found in clinical, analytical and radiological characteristics of patients in the two groups. We also compared patients with or without bronchiectasis, being pneumonia the only predictor factor of bronchiectasis. In addition, we observed a trend towards lower concentrations of IgG2 and IgG3 in patients with bronchiectasis. Finally, the functional response was evaluated, we assessed the specific Salmonella typhi antibodies, we measured pre and post vaccination concentrations in adult patients with PID. None of the patients on substitute treatment responded to the vaccine. Patients without treatment and with clinically stability responded more frequently than those symptomatic.… Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Pedro-Botet Montoya, Ma Luisa (director), true (authorsendemail).

Subjects/Keywords: Immunideficiència comú variable; Inmunodeficiencia común variable; Common variable immunodeficiency; Deficiència; Deficiencia; Deficiency; Subclasses de IgG; Deficiencia de subclases de IgG; IgG subclass deficiency; Ciències de la Salut; 616

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mateu Pruñonosa, L. (2017). Caracterización clínica e inmunológica de la inmunodeficiencia común variable, la deficiencia de subclases de Inmunoglobulina G y la deficiencia de Inmunoglobulina A en adultos. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/457985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mateu Pruñonosa, Lourdes. “Caracterización clínica e inmunológica de la inmunodeficiencia común variable, la deficiencia de subclases de Inmunoglobulina G y la deficiencia de Inmunoglobulina A en adultos.” 2017. Thesis, Universitat Autònoma de Barcelona. Accessed July 17, 2019. http://hdl.handle.net/10803/457985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mateu Pruñonosa, Lourdes. “Caracterización clínica e inmunológica de la inmunodeficiencia común variable, la deficiencia de subclases de Inmunoglobulina G y la deficiencia de Inmunoglobulina A en adultos.” 2017. Web. 17 Jul 2019.

Vancouver:

Mateu Pruñonosa L. Caracterización clínica e inmunológica de la inmunodeficiencia común variable, la deficiencia de subclases de Inmunoglobulina G y la deficiencia de Inmunoglobulina A en adultos. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2017. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/10803/457985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mateu Pruñonosa L. Caracterización clínica e inmunológica de la inmunodeficiencia común variable, la deficiencia de subclases de Inmunoglobulina G y la deficiencia de Inmunoglobulina A en adultos. [Thesis]. Universitat Autònoma de Barcelona; 2017. Available from: http://hdl.handle.net/10803/457985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Marques, Ana Lúcia Silveira Lessa. Aquisição passiva de anticorpos IgG maternos reativos com os lipopolissacarídeos de enterobactérias incidentes em infecções neonatais por recém-nascidos pré-termos e a termo.

Degree: Mestrado, Biologia da Relação Patógeno-Hospedeiro, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-16072009-152507/ ;

►

As espécies Klebsiella pneumoniae, Escherichia coli e Pseudomonas aeruginosa são responsáveis por infecções neonatais hospitalares. Lipopolissacarídeo (LPS) é o principal indutor de respostas inflamatórias. Os… (more)

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As espécies Klebsiella pneumoniae, Escherichia coli e Pseudomonas aeruginosa são responsáveis por infecções neonatais hospitalares. Lipopolissacarídeo (LPS) é o principal indutor de respostas inflamatórias. Os objetivos foram avaliar a transferência placentária de IgG reativa ao LPS de K. pneumoniae, E. coli O111, O26 e O6 e P. aeruginosa empregando ELISA para dosar IgG em soro materno e de cordão de 29 neonatos pré-termos e 32 a termo; analisar IgM total e específica no soro materno; e investigar a influência das patologias apresentadas pelas mães na transferência placentária. Concentrações de IgG total foram reduzidas em pré-termos como esperado, porem índices de transferência placentária de IgG total e IgG anti-LPS foram sistematicamente reduzidos quando comparados aos neonatos a termo. Níveis de IgM total e anti-LPS foram equivalentes em mães de ambos os grupos. As patologias das mães influenciaram os níveis de IgM no grupo de mães de pré-termos. Estes resultados indicam uma imunidade adquirida deficiente pelo grupo pré-termo aumentando os riscos de infecção.

Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa species are responsible for neonatal nosocomial infections. Bacterial lipopolysaccharide (LPS) is the major inducer of the inflammatory responses. The aims were to evaluate the placental transfer of IgG reactive to LPS present in K. pneumoniae, in E. coli O111, O26 and O6 and in P. aeruginosa employing ELISA to detect IgG in maternal and cord sera from 29 preterm and 32 term neonates; to analyze total and specific IgM on the mothers sera; and to investigate the influence of the pathologies presented by some mothers in the placental transfer. Total IgG concentrations were reduced in preterm neonates as expected, but placental transfer indexes of total and anti-LPS IgG were systematically reduced when compared with term neonates. Total and anti-LPS IgM levels were equivalent on mothers of both groups. The mothers pathologies influenced only the IgM levels in the preterm mothers group. These results indicate a deficient acquired immunity by the preterm group increasing the risk of infection.

Advisors/Committee Members: Sampaio, Magda Maria Sales Carneiro.

Subjects/Keywords: Antibodies IgG; Anticorpos IgG; Bacterial infections; Enterobacteria; Enterobactérias; Infecções bacterianas; Lipopolissacarídeos; Lipopolysaccharides; Newborn; Parasitologia; Parasitology; Recém-nascido; Transferência transplacentária; Transplacental transfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marques, A. L. S. L. (2009). Aquisição passiva de anticorpos IgG maternos reativos com os lipopolissacarídeos de enterobactérias incidentes em infecções neonatais por recém-nascidos pré-termos e a termo. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42135/tde-16072009-152507/ ;

Chicago Manual of Style (16^th Edition):

Marques, Ana Lúcia Silveira Lessa. “Aquisição passiva de anticorpos IgG maternos reativos com os lipopolissacarídeos de enterobactérias incidentes em infecções neonatais por recém-nascidos pré-termos e a termo.” 2009. Masters Thesis, University of São Paulo. Accessed July 17, 2019. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-16072009-152507/ ;.

MLA Handbook (7^th Edition):

Marques, Ana Lúcia Silveira Lessa. “Aquisição passiva de anticorpos IgG maternos reativos com os lipopolissacarídeos de enterobactérias incidentes em infecções neonatais por recém-nascidos pré-termos e a termo.” 2009. Web. 17 Jul 2019.

Vancouver:

Marques ALSL. Aquisição passiva de anticorpos IgG maternos reativos com os lipopolissacarídeos de enterobactérias incidentes em infecções neonatais por recém-nascidos pré-termos e a termo. [Internet] [Masters thesis]. University of São Paulo; 2009. [cited 2019 Jul 17]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-16072009-152507/ ;.

Council of Science Editors:

Marques ALSL. Aquisição passiva de anticorpos IgG maternos reativos com os lipopolissacarídeos de enterobactérias incidentes em infecções neonatais por recém-nascidos pré-termos e a termo. [Masters Thesis]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/42/42135/tde-16072009-152507/ ;

22. Marassa, Ana Maria. Identificação de fonte sanguínea em dípteros da Família Culicidae, em áreas de epizootia da febre amarela silvestre.

Degree: PhD, Epidemiologia, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/6/6132/tde-20072009-153444/ ;

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A importância em conhecer o padrão alimentar em mosquitos da Família Culicidae permite esclarecer alguns aspectos relacionados à transmissão de zoonoses e estimar o grau… (more)

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A importância em conhecer o padrão alimentar em mosquitos da Família Culicidae permite esclarecer alguns aspectos relacionados à transmissão de zoonoses e estimar o grau de contato humano-vetor que é fator relevante em estudos epidemiológicos. Com o objetivo de explorar o comportamento alimentar dessa Família, em área epizoótica de febre amarela silvestre, foram coletados exemplares nos municípios de Santo Antônio das Missões e Garruchos, Estado do Rio Grande do Sul. Fêmeas ingurgitadas foram obtidas por aspiração em ambiente de mata, no período de setembro de 2005 a abril de 2007 e identificadas segundo fonte de sangue ingerido através da técnica imunoenzimática ELISA de captura no sistema avidinabiotina. Foram testadas seis fontes de alimento: ave, bovino, eqüino, humano, macaco e rato. Os resultados obtidos mediante a padronização de anticorpos monoclonais possibilitaram demonstrar pela primeira vez o reconhecimento de sangue humano ingerido nesses mosquitos pelo emprego da subclasse IgG1 e comprovar a sensibilidade e especificidade da técnica ELISA de captura. No município de Santo Antônio das Missões, de um total de 190 amostras, 60,9% reagiram para sangue de boi, 23,6% para humano, 9,9% para ave, 1,9% para macaco e 3,7% para combinações de dois hospedeiros. Quanto às amostras referentes ao município de Garruchos, das 158 fêmeas capturadas na área Cachoeirinha pode-se observar reatividade para ave (16%), boi (29,6%), humano (36,8%), cavalo (4%), macaco (0,8%) e combinações de hospedeiros (12,8%), enquanto que para as 149 fêmeas pertencentes à área de São José, detectou-se sangue ingerido de boi em (51,5%), ave e humano (11,5%), macaco (6,2%), cavalo (0,8%) e mistos (18,5%). Aedes scapularis, Aedes crinifer, Culex (Culex) spp., Haemagogus leucocelaenus apresentaram maior número de fêmeas ingurgitadas nos dois municípios. Os resultados obtidos com Aedes scapularis sugerem ecletismo, conforme combinações detectadas em amostras de sangue de diferentes fontes. Haemagogus leucocelaenus apresentou a maior proporção de amostras contendo sangue humano em relação às demais fontes e essa característica traz implicações, por ser espécie incriminada na transmissão e por se tratar de área de ocorrência de epizootias de febre amarela.

The knowledge of mosquitoes Culicidae host feeding patterns permits to clarify some aspects related to zoonosis transmission and to estimate the degree of human-vector contact which is relevant in epidemiological studies. Aiming to explore the feeding behavior of these mosquitoes, specimens were collected in the municipalities of Santo Antônio das Missões and Garruchos, Rio Grande do Sul, an epizootic area of sylvatic yellow fever. Engorged females were collected by aspiration from forested areas from September 2005-April 2007 and their blood meals were identified using the avidin-biotin system of immunoenzymatic ELISA capture. Six blood meal sources were tested: bird, cattle, horse, human, monkey and rat. The result achieved with the species-specific IgG1 mAb was unprecedented for mosquito…

Advisors/Committee Members: Gomes, Almerio de Castro.

Subjects/Keywords: Culicidae; Culicidae; Feeding Habits; Hábito Alimentar; IgG; IgG; Isotipos; Isotypes; Sandwich Enzyme-Linked Immunosorbent Assay; Técnica Imunoenzimática ELISA de Captura

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marassa, A. M. (2009). Identificação de fonte sanguínea em dípteros da Família Culicidae, em áreas de epizootia da febre amarela silvestre. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-20072009-153444/ ;

Chicago Manual of Style (16^th Edition):

Marassa, Ana Maria. “Identificação de fonte sanguínea em dípteros da Família Culicidae, em áreas de epizootia da febre amarela silvestre.” 2009. Doctoral Dissertation, University of São Paulo. Accessed July 17, 2019. http://www.teses.usp.br/teses/disponiveis/6/6132/tde-20072009-153444/ ;.

MLA Handbook (7^th Edition):

Marassa, Ana Maria. “Identificação de fonte sanguínea em dípteros da Família Culicidae, em áreas de epizootia da febre amarela silvestre.” 2009. Web. 17 Jul 2019.

Vancouver:

Marassa AM. Identificação de fonte sanguínea em dípteros da Família Culicidae, em áreas de epizootia da febre amarela silvestre. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2019 Jul 17]. Available from: http://www.teses.usp.br/teses/disponiveis/6/6132/tde-20072009-153444/ ;.

Council of Science Editors:

Marassa AM. Identificação de fonte sanguínea em dípteros da Família Culicidae, em áreas de epizootia da febre amarela silvestre. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/6/6132/tde-20072009-153444/ ;

23. Sampaio, Barbara Fialho Carvalho. Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana.

Degree: Mestrado, Doenças Tropicais e Saúde Internacional, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/99/99131/tde-08012013-154837/ ;

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A toxoplasmose afeta cerca de um bilhão de pessoa em todo mundo, é geralmente assintomática, apesar de doença ocular ou doenças grave e letal em… (more)

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A toxoplasmose afeta cerca de um bilhão de pessoa em todo mundo, é geralmente assintomática, apesar de doença ocular ou doenças grave e letal em fetos, pacientes com HIV e transplantados. A sorologia é a principal ferramenta para o diagnóstico e determinação de incidência, que é uma tarefa difícil, devido à alta prevalência na maioria dos países. Estudos de incidência são ideais em crianças, mas este grupo é protegido pela sociedade e de difícil abordagem por métodos invasivos como a punção venosa. A saliva pode ser uma ótima alternativa por sua coleta não ser invasiva, aceitável para crianças, e conter pequenas quantidades de IgG, eliminada através da mucosa gengival e fluido crevicular. Métodos de detcção de anticorpos disponíveis no mercado estão focados em amostras de soro, com baixa sensibilidade e são raros os relatos de pesquisas com material biológico alternativo, como a saliva. Sendo assim, padronizamos imunoensaios com alta sensibilidade para detecção de anticorpos anti- T. gondii na saliva frente a amostras de soro de 20 voluntários adultos. A sensibilidade e especificidade dos nossos dot-ELISA e ELISA de captura com proteína A foram semelhantes entre soro e saliva. Também testamos 100 amostras de saliva de universitários em nossos ensaios, onde mostramos uma frequência da toxoplasmose de 19% (IC 95% 12-28%). Imunoensaios para detecção de IgG anti-T. gondii em saliva são uma ferramenta muito promissora para estudos epidemiológicos da toxoplasmose em crianças ou outros grupos protegidos.

Toxoplasmosis that affects about one billion people worldwide is usually asymptomatic, despite ocular disease and severe and lethal disease in fetuses, AIDS patients and transplant recipients. Serology is the main approach for diagnosis and incidence determination is a difficult task due to high prevalence in most countries. Incidence studies are feasible in children but this age group is protected and difficult to approach by invasive methods as venipuncture. Saliva could be obtained by non-invasive procedure, acceptable for children, and it contains small amounts of IgG from mucosal and gingival crevicular fluid. Available antibody detection methods are focused in serum samples, with low sensitivity and few reports of alternative biological material, like saliva. Here, we standardized immunoassays with high sensitivity for detection of anti-T. gondii IgG in paired saliva and serum sample from 20 adult volunteers, which allows DOT-ELISA and a Protein A IgG capture assay. The sensitivity and specificity of the saliva DOT-ELISA were similar to sera ELISA. We also tested 100 saliva samples from university graduates in all assays, showing 19% (95%CI 12-28%) frequency of toxoplasmosis in this group, lower than reported for our area. Protein A IgG capture saliva assay was also efficient with similar results. Immunoassay with saliva IgG for toxoplasmosis is a very promising tool for use for the epidemiology of toxoplasmosis in children or other protected groups.

Advisors/Committee Members: Andrade Junior, Heitor Franco de.

Subjects/Keywords: Detecção de IgG; dot-ELISA; dot-ELISA; IgG detection; Immunoassays; Imunoensaios; Protein A; Proteína A; Saliva; Saliva; Toxoplasmose (diagnóstico); Toxoplasmosis(diagnostic)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sampaio, B. F. C. (2012). Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/99/99131/tde-08012013-154837/ ;

Chicago Manual of Style (16^th Edition):

Sampaio, Barbara Fialho Carvalho. “Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana.” 2012. Masters Thesis, University of São Paulo. Accessed July 17, 2019. http://www.teses.usp.br/teses/disponiveis/99/99131/tde-08012013-154837/ ;.

MLA Handbook (7^th Edition):

Sampaio, Barbara Fialho Carvalho. “Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana.” 2012. Web. 17 Jul 2019.

Vancouver:

Sampaio BFC. Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2019 Jul 17]. Available from: http://www.teses.usp.br/teses/disponiveis/99/99131/tde-08012013-154837/ ;.

Council of Science Editors:

Sampaio BFC. Desenvolvimento de ensaios imunoenzimáticos para otimização da detecção de IgG anti - T.gondii em saliva humana. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/99/99131/tde-08012013-154837/ ;

Universidade Federal de Santa Maria

24. Endrigo Pompermayer. SENSIBILIDADE E ESPECIFICIDADE DO TESTE DA TURVAÇÃO PELO SULFATO DE ZINCO EM POTROS NEONATOS.

Degree: 2011, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3629

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Passive immunity acquired by the newborn foal through the colostrum is essential to prevent perinatal infections. The aim of this study was to establish the… (more)

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Passive immunity acquired by the newborn foal through the colostrum is essential to prevent perinatal infections. The aim of this study was to establish the sensitivity and specificity of the zinc sulfate turbidity test (ZST) 12 hours after first suckling for the detection of immunoglobulins (IgG) to indicate or not the failure of passive immunity transfer. There was no difference in the ZST results obtained from blood collected at 12 (n=110) and 18 (n=38) hours, confirming that the test may be performed as early as 12 hours after the first colostrum intake. When compared to the single radial immunodiffusion test, the ZST test showed 76.50%, 95.65% and 94.68% sensitivity and 93.75%, 93.94% and 75.92% specificity, respectively, for a standard of 400, 600 and 800 mg dL-1 of immunoglobulins (IgG). The results of the ZST test, i.e. according to label instructions, performed 12 hours after the first suckling at 37 °C is a reliable diagnostic tool for detecting IgG levels in newborn foals.

A imunidade passiva adquirida pelo potro neonato através da ingestão do colostro é essencial para a prevenção das infecções neonatais. O objetivo deste trabalho foi determinar a sensibilidade e a especificidade do teste da turvação pelo sulfato de zinco (TSZ) 12 horas após a primeira ingestão de colostro para detecção de imunoglobulinas G (IgG) para indicar ou não a deficiência de transferência da imunidade passiva. Não houve diferença nos resultados do teste TSZ com amostras séricas de potros PSC coletadas 12 (n=110) ou 18h (n=38) após a primeira ingestão do colostro, confirmando que o teste pode ser conduzido 12h após a ingestão de colostro. Quando comparado com a imunodifusão radial simples, o teste TSZ apresentou sensibilidade de 76,50%, 95,65% e de 94,68% e especificidade 93,75%, 93,94% e de 75,92%, para padrões de 400, 600 e 800mg dL-1 de IgG, respectivamente. Assim, o TSZ é um valioso teste para o diagnóstico no campo, desde que sejam seguidas estritamente as indicações do fabricante, especialmente quanto à temperatura. O teste pode e deve ser realizado 12 horas após a primeira mamada, pois apresenta bom índice de precisão.

Advisors/Committee Members: Mara Iolanda Batistella Rubin, Karin Erica Brass, Rodrigo Costa Mattos.

Subjects/Keywords: TSZ; colostro; imunoglobulina; IgG; equino; MEDICINA VETERINARIA; IgG; equine; imunidade passiva; immunoglobulin; colostrum; ZST; passive immunity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pompermayer, E. (2011). SENSIBILIDADE E ESPECIFICIDADE DO TESTE DA TURVAÇÃO PELO SULFATO DE ZINCO EM POTROS NEONATOS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3629

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pompermayer, Endrigo. “SENSIBILIDADE E ESPECIFICIDADE DO TESTE DA TURVAÇÃO PELO SULFATO DE ZINCO EM POTROS NEONATOS.” 2011. Thesis, Universidade Federal de Santa Maria. Accessed July 17, 2019. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3629.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pompermayer, Endrigo. “SENSIBILIDADE E ESPECIFICIDADE DO TESTE DA TURVAÇÃO PELO SULFATO DE ZINCO EM POTROS NEONATOS.” 2011. Web. 17 Jul 2019.

Vancouver:

Pompermayer E. SENSIBILIDADE E ESPECIFICIDADE DO TESTE DA TURVAÇÃO PELO SULFATO DE ZINCO EM POTROS NEONATOS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2011. [cited 2019 Jul 17]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3629.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pompermayer E. SENSIBILIDADE E ESPECIFICIDADE DO TESTE DA TURVAÇÃO PELO SULFATO DE ZINCO EM POTROS NEONATOS. [Thesis]. Universidade Federal de Santa Maria; 2011. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3629

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Oliveira-mendes, Andresa Pereira de. Aplicação da Citometria de Fluxo no Diagnótico e Critério de Cura da Leishmaniose Tegumentar Americana .

Degree: 2015, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/16876

► A leishmaniose tegumentar americana (LTA) é um problema de saúde pública, que afeta a produtividade e a vitalidade das pessoas. Embora estudos avaliem a resposta… (more)

▼ A leishmaniose tegumentar americana (LTA) é um problema de saúde pública, que afeta a produtividade e a vitalidade das pessoas. Embora estudos avaliem a resposta humoral na LTA, ainda não está completamente esclarecido o papel de anticorpos específicos na imunidade contra Leishmania. Além dos desafios sócio-econômicos que agravam ainda mais o problema da LTA, o diagnóstico da doença demonstra dificuldades, sendo freqüentemente necessário à correlação de vários elementos para se chegar ao diagnóstico definitivo. Dessa maneira, o objetivo desse estudo, foi avaliar o uso da citometria de fluxo, como uma metodologia alternativa na avaliação diagnóstica em indivíduos com LTA ativa (AT), como critério de cura pós-terapêutica em indivíduos após o tratamento (PT), naqueles com cura clínica espontânea (CE) e em indivíduos com outras doenças (doença de Chagas – DC, leishmaniose visceral–LV, hanseníase e esporotricose). A reatividade relatada pela citometria de fluxo, utilizando promastigotas vivas e fixadas de Leishmania (Viannia) braziliensis foi respectivamente, 86% e 90% de porcentagem de parasitas fluorescentes positivos (PPFP). Por análise comparativa, entre citometria de fluxo e imunofluorescência indireta, utilizando os pacientes AT, 1, 2 e 5 anos PT, verificou-se que a citometria de fluxo mostrou sensibilidade de 86% e especificidade de 77%, enquanto a IFI teve uma sensibilidade de 78% e especificidade de 85%. Contudo esta técnica teve confirmada a sua aplicabilidade no critério de cura da LTA. Analisando os resultados apresentados pelos pacientes CE, obtivemos um desempenho com 100% de especificidade. O diagnóstico diferencial da LTA que utiliza soros de pacientes DC e LV demonstrou reação cruzada, revelando resultados falso-positivos. No entanto, a utilização de soros de pacientes com esporotricose, tuberculose e hanseníase, demonstrou potencial para o uso da citometria de fluxo no diagnóstico diferencial. O estudo mostrou que os ensaios realizados utilizando anticorpos IgG, detectados por Leishmania (V.) braziliensis na citometria de fluxo, representam uma ferramenta alternativa para o diagnóstico da LTA e também abrem perspectivas para a utilização no monitoramento e critério de cura da LTA. Advisors/Committee Members: Pereira, Valéria Rêgo Alves (advisor).

Subjects/Keywords: Leishmaniose Tegumentar Americana; IgG; Citometria de fluxo; Leishmania (Viannia) braziliensis; American Tegumentary Leishmaniasis; IgG; Flow Cytometry; Leishmania (Viannia) braziliensis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira-mendes, A. P. d. (2015). Aplicação da Citometria de Fluxo no Diagnótico e Critério de Cura da Leishmaniose Tegumentar Americana . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/16876

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira-mendes, Andresa Pereira de. “Aplicação da Citometria de Fluxo no Diagnótico e Critério de Cura da Leishmaniose Tegumentar Americana .” 2015. Thesis, Universidade Federal de Pernambuco. Accessed July 17, 2019. http://repositorio.ufpe.br/handle/123456789/16876.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira-mendes, Andresa Pereira de. “Aplicação da Citometria de Fluxo no Diagnótico e Critério de Cura da Leishmaniose Tegumentar Americana .” 2015. Web. 17 Jul 2019.

Vancouver:

Oliveira-mendes APd. Aplicação da Citometria de Fluxo no Diagnótico e Critério de Cura da Leishmaniose Tegumentar Americana . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2015. [cited 2019 Jul 17]. Available from: http://repositorio.ufpe.br/handle/123456789/16876.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira-mendes APd. Aplicação da Citometria de Fluxo no Diagnótico e Critério de Cura da Leishmaniose Tegumentar Americana . [Thesis]. Universidade Federal de Pernambuco; 2015. Available from: http://repositorio.ufpe.br/handle/123456789/16876

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vienna

26. Bauer, Isabel Julia. Epitope mimics for pathogenic anti-aquaporin 4 autoantibodies in Neuromyelitis optica.

Degree: 2015, University of Vienna

URL: http://othes.univie.ac.at/39176/

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Neuromyelitis optica (NMO) ist eine gegen Astrozyten gerichtete Autoimmunerkrankung des zentralen Nervensystems, die vor allem den optischen Nerv und das Rückenmark betrifft und zu Lähmungen… (more)

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Neuromyelitis optica (NMO) ist eine gegen Astrozyten gerichtete Autoimmunerkrankung des zentralen Nervensystems, die vor allem den optischen Nerv und das Rückenmark betrifft und zu Lähmungen und eingeschränkter Sehkraft führt. Die Krankheit ist gekennzeichnet durch spezifische Autoantikörper, die sich gegen konformationelle Epitope auf den extrazellulären Schleifen des astrozytären Wasserkanals Aquaporin 4 (AQP4) richten. Die vorliegende Arbeit behandelt die Identifizierung von Epitop-Imitatoren für diese AQP4- spezifischen Antikörper (NMO-IgG). Hierfür wurde eine Phagen-präsentierte 12-Mer-Peptid- Bibliothek für ein Biopanning mit aufgereinigten polyklonalen Immunglobulinen eines NMOIgG- seropositiven Patienten verwendet. Nach einer Spezifitätszunahme in drei Biopanning- Runden wurden einzelne Phagen-Klone isoliert und ihre Reaktivität mit NMO-IgG in ELISAVersuchen evaluiert. Weiters wurden die Peptide, die durch DNA-Sequenzierung der einzelnen Phagen-Klone ermittelt wurden, mit Hilfe Mimotop-basierter Computerprogramme auf humanes AQP4 abgebildet. Basierend auf den ELISA-Ergebnissen und der errechneten Lage der Mimotope wurden acht vielversprechende Kandidaten ausgewählt und in einer FACS-basierten Analyse auf ihr Potential, die Bindung von NMO-IgG an sein Antigen, AQP4, zu blockieren, untersucht. Die FACS-Experimente ergaben, dass vier Peptide dazu fähig sind, die Interaktion von NMO-IgG mit AQP4 zu reduzieren, was darauf hindeutet, dass diese Peptide in der Tat Epitope von NMO-IgG imitieren. Aufgrund seines polyklonalen Ursprungs enthält der in dieser Arbeit verwendete Antikörper-Pool nicht nur eine, sondern mehrere Subpopulationen von NMO-IgG. Hier wurden Mimotope für mindestens zwei Untergruppen von NMO-IgG gefunden: eine A-Schleifen-unabhängige und eine A-Schleifenabhängige. Die Identifizierung der vier Mimotope stellt eine solide Grundlage dar, von der ausgehend langfristig einige Anwendungen vorstellbar sind. Die Mimotope könnten die Klassifizierung der Antikörper-Pools verschiedener Patienten nach ihrer Reaktivität mit unterschiedlichen Mimotopen ermöglichen, oder für aktive Immunisierungsstrategien verwendet werden, z. B. um im Tiermodell den Effekt bestimmter NMO-IgG-Subpopulationen zu untersuchen.

Neuromyelitis optica (NMO) is a severe autoimmune astrocytopathy of the central nervous system (CNS) that preferentially affects the optic nerve and the spinal cord leading to paralysis and impaired vision. The disease is hallmarked by specific autoantibodies that target conformational epitopes composed of the extracellular loops of the astrocytic water channel aquaporin 4 (AQP4). This study addresses the identification of epitope mimics (so-called mimotopes) for these AQP4-specific autoantibodies (NMO-IgG) For this purpose, we used a random phagedisplayed 12-mer peptide library for biopanning with purified polyclonal immunoglobulin G (IgG) of an NMO-IgG-seropositive patient as target. After increasing target specificity within three rounds of panning, single phage clones…

Subjects/Keywords: 42.13 Molekularbiologie; Neuromyelitis optica / Epitop / Mimotop / Phagendisplay / Aquaporin 4 / NMO-IgG; Neuromyelitis optica / epitope / mimotope / phage display / aquaporin 4 / NMO-IgG

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bauer, I. J. (2015). Epitope mimics for pathogenic anti-aquaporin 4 autoantibodies in Neuromyelitis optica. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/39176/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bauer, Isabel Julia. “Epitope mimics for pathogenic anti-aquaporin 4 autoantibodies in Neuromyelitis optica.” 2015. Thesis, University of Vienna. Accessed July 17, 2019. http://othes.univie.ac.at/39176/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bauer, Isabel Julia. “Epitope mimics for pathogenic anti-aquaporin 4 autoantibodies in Neuromyelitis optica.” 2015. Web. 17 Jul 2019.

Vancouver:

Bauer IJ. Epitope mimics for pathogenic anti-aquaporin 4 autoantibodies in Neuromyelitis optica. [Internet] [Thesis]. University of Vienna; 2015. [cited 2019 Jul 17]. Available from: http://othes.univie.ac.at/39176/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bauer IJ. Epitope mimics for pathogenic anti-aquaporin 4 autoantibodies in Neuromyelitis optica. [Thesis]. University of Vienna; 2015. Available from: http://othes.univie.ac.at/39176/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Linköping University

27. Kärnhall, Johan. New SPR based assays for plasma protein titer determination.

Degree: Chemistry and Biology, 2011, Linköping University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70044

► Reliable analytical tools are important for time efficient and economical process development, production and batch release of pharmaceuticals. Therapeutics recovered from human plasma, called… (more)

Subjects/Keywords: SPR; Surface Plasmon Resonance; Biacore; IgG; IgG Subclass; HSA; Albumin; plasma; plasma fractionation; Bioengineering; Bioteknik; Immunology engineering; Immunteknik

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kärnhall, J. (2011). New SPR based assays for plasma protein titer determination. (Thesis). Linköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kärnhall, Johan. “New SPR based assays for plasma protein titer determination.” 2011. Thesis, Linköping University. Accessed July 17, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kärnhall, Johan. “New SPR based assays for plasma protein titer determination.” 2011. Web. 17 Jul 2019.

Vancouver:

Kärnhall J. New SPR based assays for plasma protein titer determination. [Internet] [Thesis]. Linköping University; 2011. [cited 2019 Jul 17]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kärnhall J. New SPR based assays for plasma protein titer determination. [Thesis]. Linköping University; 2011. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-70044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

28. Dal Ponte, Silvana Teixeira. Valor diagnóstico e prognóstico do CD64 na sepse.

Degree: 2015, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/129642

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INTRODUÇÃO: A sepse é uma resposta inflamatória sistêmica causada por infecção suspeita ou confirmada. As avaliações clínicas são essenciais para a sua detecção e tratamento… (more)

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INTRODUÇÃO: A sepse é uma resposta inflamatória sistêmica causada por infecção suspeita ou confirmada. As avaliações clínicas são essenciais para a sua detecção e tratamento precoce. Hemoculturas podem demorar até dois dias para produzir um resultado, e nem sempre são confiáveis. No entanto, estudos recentes sugeriram que a expressão de CD64 de neutrófilos pode ser uma alternativa sensível e específica para o diagnóstico de uma infecção sistêmica. OBJETIVO: Analisar a diferença de valores entre CD64 de indivíduos com síndrome de resposta inflamatória sistêmica (SIRS), e sepse suspeita ou confirmada, que satisfazem os critérios de diagnóstico para SIRS ao chegar na unidade de emergência. MÉTODO: Este foi um estudo de coorte prospectivo observacional. A amostra foi composta de 109 pacientes com idade de 18 anos ou mais, com critérios de SIRS na chegada ao serviço de emergência. Expressão CD64 foi medida no prazo de 6 horas de internação, e novamente após 48 h. RESULTADOS: A análise da curva ROC sugeriu que um corte de 1.45 dos níveis de CD64 poderia diagnosticar sepse com uma sensibilidade de 0,85, especificidade de 0,75, uma precisão de 82,08%, um valor preditivo positivo de 0,964, um valor preditivo negativo de 0,375 e uma razão de verossimilhança de 3,3381. A área sob a curva foi de 0,832. CONCLUSÃO: CD64 parece ser útil como biomarcador, sensível e específico para discriminar entre SRIS e sépsis.

INTRODUCTION: Sepsis is a systemic inflammatory response to suspected or confirmed infection. Clinical evaluations are essential for its early detection and treatment. Blood cultures may take as long as two days to yield a result, and are not always reliable. However, recent studies have suggested that neutrophil CD64 expression may be a sensitive and specific alternative for the diagnosis of systemic infection. OBJECTIVE: To analyze the difference in CD64 values between subjects with systemic inflammatory response syndrome (SIRS), suspected or confirmed sepsis, who meet diagnostic criteria for SIRS upon arriving at an emergency unit. METHOD: This was a prospective observational cohort study. The sample consisted of 109 patients aged 18 years with criteria for SIRS on arrival to Emergency department. CD64 expression was measured within 6 hours of hospital admission, and once again after 48 h. RESULTS: ROC curve analysis suggested that a cutoff of 1.45 for CD64 expression could diagnose sepsis with a sensitivity of 0.85, a specificity of 0.75, an accuracy of 82.08%, a positive predictive value of 0.964, a negative predictive value of 0.375 and a positive likelihood ratio of 3.3381. The area under the curve was 0.832. CONCLUSION: CD64 appears to be a useful, sensitive and specific biomarker in discriminating between SIRS and sepsis.

Advisors/Committee Members: Goldani, Luciano Zubaran.

Subjects/Keywords: SIRS; Receptores de IgG; Sepsis; Sepse; Biomarkers; CD64

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dal Ponte, S. T. (2015). Valor diagnóstico e prognóstico do CD64 na sepse. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/129642

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dal Ponte, Silvana Teixeira. “Valor diagnóstico e prognóstico do CD64 na sepse.” 2015. Thesis, Universidade do Rio Grande do Sul. Accessed July 17, 2019. http://hdl.handle.net/10183/129642.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dal Ponte, Silvana Teixeira. “Valor diagnóstico e prognóstico do CD64 na sepse.” 2015. Web. 17 Jul 2019.

Vancouver:

Dal Ponte ST. Valor diagnóstico e prognóstico do CD64 na sepse. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2015. [cited 2019 Jul 17]. Available from: http://hdl.handle.net/10183/129642.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dal Ponte ST. Valor diagnóstico e prognóstico do CD64 na sepse. [Thesis]. Universidade do Rio Grande do Sul; 2015. Available from: http://hdl.handle.net/10183/129642

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

29. Poen, M.J. Influence of a single-dose analgesic in sows post-partum on piglet growth and IgG level.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/302272

► The amount of colostrum newborn piglets ingest in the first 24h after birth is of vital importance for their growth and the development of their… (more)

▼ The amount of colostrum newborn piglets ingest in the first 24h after birth is of vital importance for their growth and the development of their immunity system during the first weeks of life. Colostrum does not only provide them with a high dose of energy and nutrients but contains a very high concentration of IgG antibodies as well. Treating post-partum sows with an analgesic might enhance the sow’s welfare and hence enlarge the amount of colostrum intake by its piglets. In this clinical trial, the influence of injecting a single dose Meloxicam (Novem®) to farrowing sows post-partum on piglet bodyweight and serological IgG concentration is studied. Sows included (n=30) farrowed at the same day on the same farrow-to-finish farm in The Netherlands and were divided into two groups, a trial group (n=12) that received Meloxicam 20mg/ml (Novem®) and a control group (n=18) that did not receive any treatment. In the larger part of the litters, the piglets received an individual ID number, this enabled paired analysis on weight data from different points in time for 129 control and 120 trial piglets. Piglets were weighed at days 3 and 18 and serological IgG concentrations were quantified in 249 piglets at day 3 using Pig IgG ELISA (Bethyl Laboratories®). All data on weight and IgG-titre were analysed with SPSS21 Mixed ANOVA. The weight at 3 days appeared to be significantly (p=0,002) higher in the Meloxicam treated group (mean 1,88kg) than in the control group (mean 1,70kg). However, the observed higher mean on day 18 (trial:5,54kg, control:5,53kg) was not significantly different (0,234). The logIgG-values on day 3 (trial:4,54, control:4,10) were also not significantly different (p=0,099) between the two groups. Finally, as piglets might benefit from the analgesic in being able to ingest more colostrum, less spread of IgG titres within these litters could be expected. In this study, the variation coefficient did not appear to differ significantly (p=0,051) between the trial (VC=18,7%) and control (VC=29,8%) group. However, the p-value being below 0,1 did show a strong statistical trend towards significance. In conclusion of these results, administering Novem® to post-partum sows enlarges piglet weight at 3 days of age, possibly due to the intake of an enlarged amount of colostrum. This difference in weight did not show to be consistent in time, no significant differences could be found by day 18. As for piglet IgG titres, treatment led to two statistical trends to significance, the first in enlarging IgG concentration and secondly in lowering the variation of IgG titres within trial litters.The amount of colostrum newborn piglets ingest in the first 24h after birth is of vital importance for their growth and the development of their immunity system during the first weeks of life. Colostrum does not only provide them with a high dose of energy and nutrients but contains a very high concentration of IgG antibodies as well. Treating post-partum sows with an analgesic might enhance the sow’s welfare and hence enlarge the… Advisors/Committee Members: Stegeman, Prof.Dr. J.A..

Subjects/Keywords: innate immunity; pigs; IgG; piglet growth; Meloxicam; post partum analgesics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Poen, M. J. (2014). Influence of a single-dose analgesic in sows post-partum on piglet growth and IgG level. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/302272

Chicago Manual of Style (16^th Edition):

Poen, M J. “Influence of a single-dose analgesic in sows post-partum on piglet growth and IgG level.” 2014. Masters Thesis, Universiteit Utrecht. Accessed July 17, 2019. http://dspace.library.uu.nl:8080/handle/1874/302272.

MLA Handbook (7^th Edition):

Poen, M J. “Influence of a single-dose analgesic in sows post-partum on piglet growth and IgG level.” 2014. Web. 17 Jul 2019.

Vancouver:

Poen MJ. Influence of a single-dose analgesic in sows post-partum on piglet growth and IgG level. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2019 Jul 17]. Available from: http://dspace.library.uu.nl:8080/handle/1874/302272.

Council of Science Editors:

Poen MJ. Influence of a single-dose analgesic in sows post-partum on piglet growth and IgG level. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/302272

Universiteit Utrecht

30. Diebolder, C.A. Structural studies by cryo-electron tomography of two initiation complexes of the immune system: C1-IgG6 and NAIP5-NLRC4.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/321426

► Complement activation by antibodies bound to pathogens, tumors, and self antigens is a critical feature of natural immune defense, a number of disease processes, and… (more)

▼ Complement activation by antibodies bound to pathogens, tumors, and self antigens is a critical feature of natural immune defense, a number of disease processes, and immunotherapies. How antibodies activate the complement cascade, however, is poorly understood. We found that speciﬁc noncovalent interactions between Fc segments of immunoglobulin G (IgG) antibodies resulted in the formation of ordered antibody hexamers after antigen binding on cells. These hexamers recruited and activated C1, the ﬁrst component of complement, thereby triggering the complement cascade. The interactions between neighboring Fc segments could be manipulated to block, reconstitute, and enhance complement activation and the killing of target cells, using all four human IgG subclasses. We oﬀer a general model for understanding antibody-mediated complement activation and the design of antibody therapeutics with enhanced eﬃcacy. Inflammasomes are high molecular weight protein complexes that play a crucial role in innate immunity by activating caspase-1. Inflammasome formation is initiated when molecules originating from invading microorganisms activate NLRs and induce NLR multimerization. Little is known about the conformational changes involved in NLR activation and the structural organization of NLR multimers. Here we show by cryo-electron tomography that flagellin-induced NAIP5/NLRC4 multimers form right- and left-handed helical polymers with a diameter of 28 nm and a rise of 6.5 nm. Sub tomogram averaging produced an electron density map at 4 nm resolution, which was used for rigid body fitting of NLR subdomains derived from the crystal structure of dormant NLRC4. The resulting structural model of inflammasome-incorporated NLRC4 indicates that a prominent rotation of the LRR domain of NLRC4 is necessary for multimer formation, providing unprecedented insight in the conformational changes that accompany NLR activation. The resolution of electron tomograms is anisotropic due to geometrical constraints during data collection, such as the limited tilt range and single-axis tilt series acquisition. Acquisition of dual axis-tilt series can decrease these effects. However, in cryo-electron tomography, to limit the electron radiation damage that occurs during imaging, the total dose should not increase and must be fractionated over the two tilt series. Here, we set out to determine whether it is beneficial to fractionate electron dose for recording dual-axis cryo-electron tilt series or whether it is better to perform single-axis acquisition. To assess the quality of tomographic reconstructions in different directions, we introduce conical Fourier shell correlation (cFSCe/o). Employing cFSCe/o, we compared the resolution isotropy of single-axis and dual-axis (cryo-)electron tomograms using even/odd split data sets. We show that the resolution of dual-axis simulated and cryo-electron tomograms in the plane orthogonal to the electron beam becomes more isotropic compared to single-axis tomograms and high-resolution peaks along the tilt axis disappear.… Advisors/Committee Members: Gros, Piet, Koster, A.J., Koning, Roman I.

Subjects/Keywords: cryo-electron tomography; complement; IgG-Hexamer; inflammasome; conical Fourier shell correlation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Diebolder, C. A. (2015). Structural studies by cryo-electron tomography of two initiation complexes of the immune system: C1-IgG6 and NAIP5-NLRC4. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/321426

Chicago Manual of Style (16^th Edition):

Diebolder, C A. “Structural studies by cryo-electron tomography of two initiation complexes of the immune system: C1-IgG6 and NAIP5-NLRC4.” 2015. Doctoral Dissertation, Universiteit Utrecht. Accessed July 17, 2019. http://dspace.library.uu.nl:8080/handle/1874/321426.

MLA Handbook (7^th Edition):

Diebolder, C A. “Structural studies by cryo-electron tomography of two initiation complexes of the immune system: C1-IgG6 and NAIP5-NLRC4.” 2015. Web. 17 Jul 2019.

Vancouver:

Diebolder CA. Structural studies by cryo-electron tomography of two initiation complexes of the immune system: C1-IgG6 and NAIP5-NLRC4. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2015. [cited 2019 Jul 17]. Available from: http://dspace.library.uu.nl:8080/handle/1874/321426.

Council of Science Editors:

Diebolder CA. Structural studies by cryo-electron tomography of two initiation complexes of the immune system: C1-IgG6 and NAIP5-NLRC4. [Doctoral Dissertation]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/321426

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