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You searched for subject:(IL 1b processing). Showing records 1 – 2 of 2 total matches.

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1. Buzzy, Christina Antonopoulos. NON-CANONICAL IL-1ß PROCESSING VIA CASPASE-8 IN MURINE DENDRITIC CELLS AND MACROPHAGES.

Degree: PhD, Pathology, 2015, Case Western Reserve University School of Graduate Studies

Dysregulated IL-1ß production has been implicated in a host of disease settings, ranging from autoinflammatory syndromes to diabetes and Alzheimer’s disease. The first-identified interleukin-converting enzyme or `ICE’ was caspase-1. The assembly of cytosolic, multi-protein complexes called inflammasomes canonically triggers the activation of caspase-1. Following proximity-induced autocatalysis of procaspase-1 dimers, activated caspase-1 molecules proteolytically catalyze the conversion of the pro- inflammatory cytokines, proIL-1ß and proIL-18, into their mature cytokines, IL-1ß and IL-18. As will be discussed in the following chapters, the coordination of IL-1ß cytokine generation is under the regulation of the individual proteins which comprise the particular cytosolic, multi-protein complex.Caspase-8 has recently emerged as an alternative ICE in response to microbially- induced inflammation for the generation of mature IL-1ß and IL-18. My dissertation studies contribute to this rapidly growing field in multiple unique facets. First, in the genetic absence of caspase-1, caspase-8 is recruited to an ASC-containing platform for IL-1ß processing in response to nigericin in dendritic cells (DCs). Besides acting as a direct IL-1ß convertase, caspase-8 also regulates the activation of caspase-1 induced by nigericin in LPS-primed control DCs, adding an additional layer of complexity to IL-1ß regulation. In the context of anti-cancer treatment, the commonly used pro-apoptotic chemotherapeutic drug, doxorubicin (Dox), induces a non-canonical IL-1ß processing platform, consisting of RIP1/FADD/caspase-8 and is driven by the downregulation of the cellular inhibitor of apoptosis protein 1 (cIAP1). Enzymatically active caspase-8 then promotes IL-1ß maturation within this specific ripoptosome complex. Of particular interest is how this inflammatory signaling pathway may contribute to cancer pathogenesis or amelioration during treatment with Dox.My data additionally demonstrate the intertwined nature of inflammatory and cell death signaling pathways. The activity of IL-1ß convertases, caspase-1 and caspase-8, dictates which mode of cell death a TLR4-primed macrophage/DC will undergo and is highly stimulus-dependent. Consequently, IL-1ß signaling is closely associated with pyroptosis when a caspase-1 platform is engaged and apoptotosis when a caspase-8 platform is engaged. Herein this thesis, I discriminate between caspase-1- and caspase-8- based signaling pathways in murine dendritic cells and macrophages for the proteolytic maturation of IL-1ß. Advisors/Committee Members: Dubyak, George (Advisor), Pearlman, Eric (Committee Chair).

Subjects/Keywords: Immunology; Biology; caspase-8; IL-1b processing; pro-apoptotic chemotherapeuitc drugs; NLRP3; inflammasome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Buzzy, C. A. (2015). NON-CANONICAL IL-1ß PROCESSING VIA CASPASE-8 IN MURINE DENDRITIC CELLS AND MACROPHAGES. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1417718855

Chicago Manual of Style (16th Edition):

Buzzy, Christina Antonopoulos. “NON-CANONICAL IL-1ß PROCESSING VIA CASPASE-8 IN MURINE DENDRITIC CELLS AND MACROPHAGES.” 2015. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed November 30, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1417718855.

MLA Handbook (7th Edition):

Buzzy, Christina Antonopoulos. “NON-CANONICAL IL-1ß PROCESSING VIA CASPASE-8 IN MURINE DENDRITIC CELLS AND MACROPHAGES.” 2015. Web. 30 Nov 2020.

Vancouver:

Buzzy CA. NON-CANONICAL IL-1ß PROCESSING VIA CASPASE-8 IN MURINE DENDRITIC CELLS AND MACROPHAGES. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 2015. [cited 2020 Nov 30]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1417718855.

Council of Science Editors:

Buzzy CA. NON-CANONICAL IL-1ß PROCESSING VIA CASPASE-8 IN MURINE DENDRITIC CELLS AND MACROPHAGES. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1417718855

2. Soulliere, Cheryl. Regulation of rainbow trout (Oncorhynchus mykiss) mRNA at the innate/adaptive immunity interface in response to Vibrogen 2.

Degree: 2017, University of Waterloo

Aquaculture supplies the world market with over 50% of food fish and is an economically important industry, however limited disease management strategies often lead to significant economic losses and/or poor-quality product. Historically, antibiotics were used prophylactically and as a treatment in disease outbreaks caused by bacterial infections, however, due to antibiotic resistance among pathogens alternative methods for disease control are required and strong vaccination programs are essential. Vibrogen 2, is considered a successful vaccine available for use in aquaculture, however the reasons for this success are poorly understood. Furthermore, while fish appear to have immunological machinery and signaling molecules similar to those found in mammals, evidence suggests fish may use these immune mechanisms differently. In this study, the early immune response elicited by Vibrogen 2 vaccination in rainbow trout head kidney and RTS11 cells was examined through investigation of mRNA expression of two pro-inflammatory cytokines; IL-1β and IFNγ, as well as the mRNA expression of MH class I associated TAP1 and MH class II associate S25-7, to understand the efficacy of the bacterin, as well as, the innate/adaptive immunity interface in teleosts. In RTS11 cells Vibrogen 2 up-regulated IL-1β, S25-7 and TAP1 but not IFNγ in the first 24h post-stimulation and in rainbow trout head kidney IL-1β, IFNγ and TAP1 but not S25-7 was up-regulated in the first 24h after vaccination. Moreover, at 24h S25-7 expression was trending down in head kidney. The results suggest Vibrogen 2 is initiating antibody production in rainbow trout via the pro-inflammatory pathway while the MH class I pathway is also activated. Further, it is possible that 14-1 or INVX rather than S25-7 is involved in MH class II presentation in rainbow trout head kidney. It appears both MH class I and MH class II may be involved in the development of long term immunity in fish and thus, both pathways should be considered when developing vaccines in the future.

Subjects/Keywords: innate immunity; adaptive immunity; pro-inflammatory response; S25-7; TAP1; IL-1B; IFNy; head kidney; RTS11; trout; vaccine efficacy; macrophage; inflammation; pro-inflammatory cytokines; invariant chain; gene expression; Vibriosis; Vibrio anguillarum; Vibrio ordalii; Vibrogen 2; interleukin 1 beta; interferon gamma; rainbow trout; mRNA expression; transporter associated with antigen processing 1

…68 3.3.2 Normalized ratios of IL-1β, IFNγ, S25-7 and TAP1 in Head Kidney of Rainbow Trout… …68 3.3.3 The First 24h of IL-1β, IFNγ, S25-7 and TAP1 Gene Expression in the Head Kidney… …IL-1β, IFNγ, S25-7 and TAP1 normalized ratios in RTS11 cells .. ...…46 Figure 2-3: IL… …compared to control expression levels ... …... …49 Figure 2-4: IL-1β, IFNγ, S25-7 and TAP1… …51 Figure 2-5: Summary of IL-1β, IFNγ, S25-7 and TAP1 fold-change gene expression in RTS11… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Soulliere, C. (2017). Regulation of rainbow trout (Oncorhynchus mykiss) mRNA at the innate/adaptive immunity interface in response to Vibrogen 2. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/12096

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Soulliere, Cheryl. “Regulation of rainbow trout (Oncorhynchus mykiss) mRNA at the innate/adaptive immunity interface in response to Vibrogen 2.” 2017. Thesis, University of Waterloo. Accessed November 30, 2020. http://hdl.handle.net/10012/12096.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Soulliere, Cheryl. “Regulation of rainbow trout (Oncorhynchus mykiss) mRNA at the innate/adaptive immunity interface in response to Vibrogen 2.” 2017. Web. 30 Nov 2020.

Vancouver:

Soulliere C. Regulation of rainbow trout (Oncorhynchus mykiss) mRNA at the innate/adaptive immunity interface in response to Vibrogen 2. [Internet] [Thesis]. University of Waterloo; 2017. [cited 2020 Nov 30]. Available from: http://hdl.handle.net/10012/12096.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soulliere C. Regulation of rainbow trout (Oncorhynchus mykiss) mRNA at the innate/adaptive immunity interface in response to Vibrogen 2. [Thesis]. University of Waterloo; 2017. Available from: http://hdl.handle.net/10012/12096

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.