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You searched for subject:(Hydrolyzable tannins). Showing records 1 – 2 of 2 total matches.

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1. Vidmar, Neža. Vpliv hidrolizirajočih taninov iz izvlečkov kostanja na črevesne epitelijske celice humanega in prašičjega izvora.

Degree: 2017, Univerza v Mariboru

V magistrskem delu smo ugotavljali vpliv različnih koncentracij kostanjevih taninov na presnovo celic in izražanje toksičnih učinkov na epitelijske celice ter izražanje pozitivnih učinkov kostanjevih taninov na tesne celične stike med epitelijskimi celicami tankega črevesja humanega in prašičjega izvora. Za citotoksični test, test presnovne aktivnosti celic, test viabilnosti celic in funkcionalne celične 3-D modele smo uporabili prašičje enterocite CLAB in humane fetalne epitelijske celice tankega črevesja H4-1 ter hidrolizirajoče tanine iz izvlečkov kostanja, FARMATAN in FARMATAN ET 50. Pri izpostavitvi funkcionalnih celičnih 3D-modelov taninom in merjenju transepitelijske električne upornosti (TEER) smo uporabili elektrodo za merjenje električne upornosti in potenciala. V celicah, ki so bile izpostavljene višjim koncentracijam kostanjevih taninov, je prišlo do zmanjšane količine kristal vijoličnega. Pri presnovni aktivnosti celic so se pri višjih koncentracijah vrednosti absorbance v primerjavi s kontrolo znižale. Po izpostavitvi celičnih linij kostanjevemu taninu so vrednosti transepitelijske električne upornosti (TEER) glede na kontrolo naraščale.

In this thesis, the effect of different concentrations of chestnut tannins on the metabolism of cells and the expression of the toxic effects on epithelial cells, and expression of the positive effects of chestnut tannins on tight junctions between epithelial cells of the small intestine of human and porcine origin was studied. For the cytotoxic test, the metabolic activity of cells, cell viability test and functional 3D cell models, porcine enterocytes CLAB and human fetal epithelial cells of the small intestine H4-1 and hydrolyzable tannin extracts of chestnut, FARMATAN and FARMATAN ET 50 were used. For the exposure of functional 3D cell models to tannins and for measuring of transepithelial resistance, electrode for measuring the electrical resistance and potential was used. When cells were exposed to higher concentrations of chestnut tannins there was a reduced amount of crystal violet. In the metabolic activity of the cells at higher concentrations, absorbance values compared with control decreased. After exposure of the cell lines to chestnut tannins values of transepithelial electrical resistance (TEER) compared to the control increased.

Advisors/Committee Members: Škorjanc, Dejan.

Subjects/Keywords: hidrolizirajoči tanini; črevesje; tesni stiki; funkcionalni 3D – celični modeli; transepitelijska električna upornost (TEER); hydrolyzable tannins; gut; tight junctions; functional 3D cell models; transephitelial electrical resistance (TEER)

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APA (6th Edition):

Vidmar, N. (2017). Vpliv hidrolizirajočih taninov iz izvlečkov kostanja na črevesne epitelijske celice humanega in prašičjega izvora. (Masters Thesis). Univerza v Mariboru. Retrieved from https://dk.um.si/IzpisGradiva.php?id=66089 ; https://dk.um.si/Dokument.php?id=112216&dn=

Chicago Manual of Style (16th Edition):

Vidmar, Neža. “Vpliv hidrolizirajočih taninov iz izvlečkov kostanja na črevesne epitelijske celice humanega in prašičjega izvora.” 2017. Masters Thesis, Univerza v Mariboru. Accessed January 22, 2020. https://dk.um.si/IzpisGradiva.php?id=66089 ; https://dk.um.si/Dokument.php?id=112216&dn=.

MLA Handbook (7th Edition):

Vidmar, Neža. “Vpliv hidrolizirajočih taninov iz izvlečkov kostanja na črevesne epitelijske celice humanega in prašičjega izvora.” 2017. Web. 22 Jan 2020.

Vancouver:

Vidmar N. Vpliv hidrolizirajočih taninov iz izvlečkov kostanja na črevesne epitelijske celice humanega in prašičjega izvora. [Internet] [Masters thesis]. Univerza v Mariboru; 2017. [cited 2020 Jan 22]. Available from: https://dk.um.si/IzpisGradiva.php?id=66089 ; https://dk.um.si/Dokument.php?id=112216&dn=.

Council of Science Editors:

Vidmar N. Vpliv hidrolizirajočih taninov iz izvlečkov kostanja na črevesne epitelijske celice humanega in prašičjega izvora. [Masters Thesis]. Univerza v Mariboru; 2017. Available from: https://dk.um.si/IzpisGradiva.php?id=66089 ; https://dk.um.si/Dokument.php?id=112216&dn=


Miami University

2. Chen, Yumin. Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems.

Degree: PhD, Chemistry, 2004, Miami University

Reactive oxygen species (ROS) are produced in human metabolic processes and uncontrolled ROS are detrimental to human health. <i>In vitro</i> chemical assays show that dietary tannins have potent ROS scavenging activity. My research is designed to use model systems to reveal interactions between tannins and factors (pH and protein) found in the human digestive tract where tannins work as biological antioxidants. Pentagalloyl glucose (PGG), bovine serum albumin (BSA), and NaIO4 were chosen as the model tannin, protein and oxidant, respectively. PGG was prepared from tannic acids via a methanolysis reaction. [14C]PGG was synthesized from [U-14C]-D-glucopyranose and tri-<i>O</i>-benzylgallic acid as a radiochemical tracer. When PGG was oxidized by NaIO4, the formation of different oxidation products was controlled by the reaction pH. PGG oxidation produced an o-semiquinone radical intermediate, which formed polymeric products at low pH (e.g. pH 2.1). The <i>o</i>-semiquinone radical ionized at pH > 5 and was labile to being further oxidized to <i>o</i>-quinone. Reaction pH similarly affected the formation of oxidation products of epicatechin16 (4¿8) catechin (procyanidin) or epigallocatechin gallate (EGCG), suggesting that these three different tannins followed the same oxidation mechanism. When PGG was oxidized in the presence of BSA at various pH values, BSA promoted the formation of quinone but inhibited the formation of polymeric products. In addition to affecting PGG oxidation, BSA interacted with PGG under oxidizing conditions and formed oxidized PGG-BSA complexes. A radiochemical method was devised to quantitate oxidized PGG-BSA complexes. The complexes were treated with sodium dodecyl sulfate (SDS) to remove any non-covalently bound PGG and were co-precipitated with BSA by trichloroacetic acid (TCA) for radiochemical assessment. The molar ratio of PGG and BSA determined the solubility of oxidized PGG-BSA complexes. Soluble complexes were formed at low molar ratios (e.g. PGG/BSA=1). At high molar ratios (e.g. PGG/BSA=22), soluble complexes were rapidly converted to insoluble complexes. Besides NaIO4, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS•+) and 2,2’-azobis(isobutyramidine) dihydrochloride (AAPH) were tested as oxidants. It was found that NaIO4 and ABTS•+ induced the formation of oxidized PGG-BSA complexes more rapidly than AAPH. Advisors/Committee Members: Hagerman, Ann (Advisor).

Subjects/Keywords: Chemistry, Biochemistry; Polyphenol; Tannins; Hydrolyzable tannins; Tannin-protein interaction; Polyphenol-protein interaction; Polyphenol oxidation; Tannin oxidation; Antioxidant; Pentagalloyl glucose; Oxidized tannin-protein complexes; Oxidized polyphenol-protein comp

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Chen, Y. (2004). Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems. (Doctoral Dissertation). Miami University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925

Chicago Manual of Style (16th Edition):

Chen, Yumin. “Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems.” 2004. Doctoral Dissertation, Miami University. Accessed January 22, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925.

MLA Handbook (7th Edition):

Chen, Yumin. “Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems.” 2004. Web. 22 Jan 2020.

Vancouver:

Chen Y. Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems. [Internet] [Doctoral dissertation]. Miami University; 2004. [cited 2020 Jan 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925.

Council of Science Editors:

Chen Y. Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems. [Doctoral Dissertation]. Miami University; 2004. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925

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