subject:(Human Induced Pluripotent Stem Cells)

University of Southern California

1. Pomeroy, Jordan Elliott. Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions.

Degree: PhD, Systems Biology and Disease, 2012, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847

► Future use of pluripotent cells for regenerative medicine will require the derivation and characterization of new cell lines in clinical grade conditions. Removing potential sources… (more)

▼ Future use of pluripotent cells for regenerative medicine will require the derivation and characterization of new cell lines in clinical grade conditions. Removing potential sources of contamination, such as cell culture components sourced from animals, will aid the regulatory approval for future stem cell based therapeutics. In this dissertation, I describe the development of a xenobiotic-free cell culture system for the derivation and maintenance of human pluripotent cell lines. I have derived >40 hiPSC lines and one hESC line in the xeno-free cell culture conditions with maintenance of pluripotency charcacterized by morphology and immunocytochemistry marker expression for >50 passages and >30 passages respectively. The hESC line, USC-01 and several hiPSC lines derived for this study demonstrate highly similar gene expression patterns although slight differences are apparent. USC-01 and several hiPSC lines demonstrate the ability to differentiate into cells displaying characteristics of all three germ layers in an embryoid body differentiation assay. Further examination of the process of reprogramming somatic cells to a pluripotent state at both the RNA and protein expression levels indicates several genes/markers selective for hiPSCs achieving a pluripotent state most similar to the “gold-standard” of pluripotency possessed by hESCs. This study confirms the usefulness of the markers TRA-1-60, E-Cadherin and EpCAM for live-cell selection of the best hiPSC colonies and also demonstrates the usefulness of the marker GCTM-2. Expression analysis of colonies undergoing reprogramming also indicates that the genes FOXD3, CDH3, LCK, EDNRB, EPHA1, SOX2, and HAS3 are active in only a small subset of colonies 30 days after transfection of the piggyBac transposon reprogramming cassette. Since these genes are active in all hESC and most hiPSC positive control lines tested, confirmation of their activation could be used to select reprogramming hiPSC colonies most likely to achieve a pluripotent state similar to hESCs. Increased selection and derivation efficiencies of hiPSC lines demonstrating high fidelity to the hESC pluripotent state will streamline the generation of hiPSC lines for future testing as a replacement for hESCs in regenerative medicine. Advisors/Committee Members: Pera, Martin F. (Committee Chair), Ying, Qi-Long (Committee Member), Lu, Wange (Committee Member), Chuong, Cheng-Ming (Committee Member).

Subjects/Keywords: embryonic; stem; induced; pluripotent; cells; clinical; human

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APA (6^th Edition):

Pomeroy, J. E. (2012). Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847

Chicago Manual of Style (16^th Edition):

Pomeroy, Jordan Elliott. “Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions.” 2012. Doctoral Dissertation, University of Southern California. Accessed January 20, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847.

MLA Handbook (7^th Edition):

Pomeroy, Jordan Elliott. “Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions.” 2012. Web. 20 Jan 2021.

Vancouver:

Pomeroy JE. Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions. [Internet] [Doctoral dissertation]. University of Southern California; 2012. [cited 2021 Jan 20]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847.

Council of Science Editors:

Pomeroy JE. Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions. [Doctoral Dissertation]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847

University of Georgia

2. Avery, John Welch. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.

Degree: 2018, University of Georgia

URL: http://hdl.handle.net/10724/37263

► Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling… (more)

▼ Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling the development of embryonic and adult tissues. In this work, we attempted a patient-specific disease in a dish model of the craniofacial disorder Treacher Collins Syndrome (TCS) and the directed differentiation of brown adipocytes from hPSCs. The TCS model was unable to replicate the expected phenotype of reduced neural crest cell numbers or migratory potential. This shortcoming has been putatively attributed to reducing and high antioxidant conditions of the culture medium. Modulating brown adipocyte activity in humans has become an attractive therapeutic target for obesity and related metabolic disorders such as type 2 diabetes. Murine models suggest the brown adipose organ is capable of significant changes in body mass and circulating levels of insulin and glucose. Methods to interrogate human brown adipocyte functionality have been based on adult primary cells or overexpression systems. Here we report a robust and efficient chemically-defined method to differentiate human pluripotent stem cells into brown adipocytes through a developmentally appropriate paraxial mesoderm intermediate. These adipocytes display characteristics of mature brown adipocytes including marker expression, responsiveness to stimulation, enhanced metabolic activity, thermogenic capacity, lipolysis, and utilization of fatty acids. These cells are capable of being maintained in culture for several weeks, are amenable to passage, and upon transplantation in mice can give rise to adipose depots that maintain both morphological and functional properties. We propose that these cells are appropriate for modeling brown adipocyte development in vivo, elucidating basic biological cell fate decisions and branch points and as a platform for high-throughput drug screening to identify putative therapeutic targets to combat obesity and related disorders.

Subjects/Keywords: Brown adipocytes; human pluripotent stem cells, induced pluripotent stem cells, neural crest cells, paraxial mesoderm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Avery, J. W. (2018). Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/37263

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Thesis, University of Georgia. Accessed January 20, 2021. http://hdl.handle.net/10724/37263.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Web. 20 Jan 2021.

Vancouver:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Internet] [Thesis]. University of Georgia; 2018. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10724/37263.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Thesis]. University of Georgia; 2018. Available from: http://hdl.handle.net/10724/37263

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

3. Avery, John Welch. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.

Degree: 2018, University of Georgia

URL: http://hdl.handle.net/10724/37106

► Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling… (more)

▼ Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling the development of embryonic and adult tissues. In this work, we attempted a patient-specific disease in a dish model of the craniofacial disorder Treacher Collins Syndrome (TCS) and the directed differentiation of brown adipocytes from hPSCs. The TCS model was unable to replicate the expected phenotype of reduced neural crest cell numbers or migratory potential. This shortcoming has been putatively attributed to reducing and high antioxidant conditions of the culture medium. Modulating brown adipocyte activity in humans has become an attractive therapeutic target for obesity and related metabolic disorders such as type 2 diabetes. Murine models suggest the brown adipose organ is capable of significant changes in body mass and circulating levels of insulin and glucose. Methods to interrogate human brown adipocyte functionality have been based on adult primary cells or overexpression systems. Here we report a robust and efficient chemically-defined method to differentiate human pluripotent stem cells into brown adipocytes through a developmentally appropriate paraxial mesoderm intermediate. These adipocytes display characteristics of mature brown adipocytes including marker expression, responsiveness to stimulation, enhanced metabolic activity, thermogenic capacity, lipolysis, and utilization of fatty acids. These cells are capable of being maintained in culture for several weeks, are amenable to passage, and upon transplantation in mice can give rise to adipose depots that maintain both morphological and functional properties. We propose that these cells are appropriate for modeling brown adipocyte development in vivo, elucidating basic biological cell fate decisions and branch points and as a platform for high-throughput drug screening to identify putative therapeutic targets to combat obesity and related disorders.

Subjects/Keywords: Brown adipocytes; human pluripotent stem cells, induced pluripotent stem cells, neural crest cells, paraxial mesoderm

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Avery, J. W. (2018). Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/37106

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Thesis, University of Georgia. Accessed January 20, 2021. http://hdl.handle.net/10724/37106.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Web. 20 Jan 2021.

Vancouver:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Internet] [Thesis]. University of Georgia; 2018. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10724/37106.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Thesis]. University of Georgia; 2018. Available from: http://hdl.handle.net/10724/37106

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Paredes, Maria Guadalupe. Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine.

Degree: Biomedical Engineering, 2018, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:792836/

► Ischemic heart disease is the leading cause of death globally, which takes more than 17.7 million lives every year (WHO, 2018), yet current treatments and… (more)

Subjects/Keywords: induced pluripotent stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paredes, M. G. (2018). Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792836/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paredes, Maria Guadalupe. “Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine.” 2018. Thesis, Brown University. Accessed January 20, 2021. https://repository.library.brown.edu/studio/item/bdr:792836/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paredes, Maria Guadalupe. “Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine.” 2018. Web. 20 Jan 2021.

Vancouver:

Paredes MG. Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine. [Internet] [Thesis]. Brown University; 2018. [cited 2021 Jan 20]. Available from: https://repository.library.brown.edu/studio/item/bdr:792836/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paredes MG. Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792836/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

5. Wendel, Jacqueline. Engineered Cardiac Tissues for Delivery of Cells to the Injured Myocardium.

Degree: PhD, Biomedical Engineering, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/175369

► With the high incidence of heart failure in the developing world and the inherent risks and limited availability of donor hearts, cell-based solutions have become… (more)

Subjects/Keywords: Cardiac; Human Induced Pluripotent Stem Cells; Myocardial Infarction; Preclinical; Tissue Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wendel, J. (2015). Engineered Cardiac Tissues for Delivery of Cells to the Injured Myocardium. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/175369

Chicago Manual of Style (16^th Edition):

Wendel, Jacqueline. “Engineered Cardiac Tissues for Delivery of Cells to the Injured Myocardium.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 20, 2021. http://hdl.handle.net/11299/175369.

MLA Handbook (7^th Edition):

Wendel, Jacqueline. “Engineered Cardiac Tissues for Delivery of Cells to the Injured Myocardium.” 2015. Web. 20 Jan 2021.

Vancouver:

Wendel J. Engineered Cardiac Tissues for Delivery of Cells to the Injured Myocardium. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11299/175369.

Council of Science Editors:

Wendel J. Engineered Cardiac Tissues for Delivery of Cells to the Injured Myocardium. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/175369

Penn State University

6. Lin, Tzu Ting. DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS.

Degree: 2017, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/13797txl5286

► Owing to their ability to differentiate into all cell types in body, and therefore greatly impact the landscape of regenerative medicine and tissue engineering, the… (more)

Subjects/Keywords: Stem cell culture medium; LaSR; Xeno-free medium; Stem cell culture; Human induced pluripotent stem cells; Human embryonic stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, T. T. (2017). DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/13797txl5286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Tzu Ting. “DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS.” 2017. Thesis, Penn State University. Accessed January 20, 2021. https://submit-etda.libraries.psu.edu/catalog/13797txl5286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Tzu Ting. “DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS.” 2017. Web. 20 Jan 2021.

Vancouver:

Lin TT. DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS. [Internet] [Thesis]. Penn State University; 2017. [cited 2021 Jan 20]. Available from: https://submit-etda.libraries.psu.edu/catalog/13797txl5286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin TT. DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS. [Thesis]. Penn State University; 2017. Available from: https://submit-etda.libraries.psu.edu/catalog/13797txl5286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

7. Hayworth, Miranda Kristine. Multipotent mesothelial progenitors derived from human pluripotent stem cells function in development and tissue repair.

Degree: 2016, University of Georgia

URL: http://hdl.handle.net/10724/34226

► The mesothelium, which constitutes the outermost layer of the coelomic organs including the heart, lung, liver and gut, plays a critical role in the development,… (more)

▼ The mesothelium, which constitutes the outermost layer of the coelomic organs including the heart, lung, liver and gut, plays a critical role in the development, homeostasis and potentially in repair of the internal organs following injury or disease. During organogenesis, the mesothelium contributes to the vasculature and stroma of the developing organs in addition to acting as an important mitogenic signal. While relatively quiescient in the adult, in response to injury, the mesothelial layer has been shown to reactivate its embryonic developmental program, invade the injured tissue and release cytokines that modulate the injury response. Here, we describe highly efficient methods for the differentiation of human pluripotent stem cells (hPSCs) into mesothelial progenitor cells (MPCs) and define their developmental potential in both in vitro and in vivo models. Differential gene expression analysis of freshly isolated murine embryonic mesothelium was used to validate the characterization of our hPSC-derived MPCs as authentic mesothelium. Clonogenic assays were used to determine the in vitro differentiation potential of hPSC-derived MPCs into fibroblast, smooth muscle and endothelial lineages and the multipotency of hPSC-derived MPCs was evaluated in vivo by assessing integration of hPSC-derived MPCs into embryonic chick hearts and mechanically-damaged neonatal mouse hearts. At the molecular level, hPSC-derived MPCs are indistinguishable from their in vivo counterparts and respond to signaling molecules that are known to impact mesothelial cell fate decisions during development as shown by their in vitro differentiation into fibroblasts, smooth muscle cells and endothelium in response to PDGF-alpha, PDGF-beta and Vegf signaling, respectively. When transplanted onto developing chick hearts, MPCs incorporate into the host mesothelium and invade the underlying myocardium. MPCs transplanted into mechanically-damaged neonatal mouse hearts migrate into damaged tissue along with endogenous epicardium-derived cells and assemble into coronary vessels in the repair zone. In addition to the utility of these cells for modeling mesothelial development and disease, this study opens up new avenues for tissue engineering and regeneration of the coelomic organs.

Subjects/Keywords: Human embryonic stem cells, human induced pluripotent stem cells; mesothelium; pluripotency; differentiation; regeneration; mesenchymal stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hayworth, M. K. (2016). Multipotent mesothelial progenitors derived from human pluripotent stem cells function in development and tissue repair. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/34226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hayworth, Miranda Kristine. “Multipotent mesothelial progenitors derived from human pluripotent stem cells function in development and tissue repair.” 2016. Thesis, University of Georgia. Accessed January 20, 2021. http://hdl.handle.net/10724/34226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hayworth, Miranda Kristine. “Multipotent mesothelial progenitors derived from human pluripotent stem cells function in development and tissue repair.” 2016. Web. 20 Jan 2021.

Vancouver:

Hayworth MK. Multipotent mesothelial progenitors derived from human pluripotent stem cells function in development and tissue repair. [Internet] [Thesis]. University of Georgia; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10724/34226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hayworth MK. Multipotent mesothelial progenitors derived from human pluripotent stem cells function in development and tissue repair. [Thesis]. University of Georgia; 2016. Available from: http://hdl.handle.net/10724/34226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

8. Awe, Jason Patrick. Investigating the Mechanisms of Reprogramming and Optimizing the Generation of Potentially Therapeutically Useful Induced Pluripotent Stem Cell Derivatives.

Degree: Molec & Med Pharmacology, 2014, UCLA

URL: http://www.escholarship.org/uc/item/25v79301

► Human induced pluripotent stem cells (hiPSCs), derived from easily obtainable skin cells, possess enormous opportunity for autologous cellular treatment therapies, gene correction, and disease modeling… (more)

▼ Human induced pluripotent stem cells (hiPSCs), derived from easily obtainable skin cells, possess enormous opportunity for autologous cellular treatment therapies, gene correction, and disease modeling without worries of ethical constraints associated with human embryonic stem cells (hESCs). Although lentiviral based reprogramming remains as one of the most popular methods for reprogramming, potentially oncogenic viral integrations in random locations throughout the genome along with non-human antigens associated with the reprogramming process thwart the clinical applications of these hiPSCs. To address these concerns we derived a hiPSC line void of any exogenous reprogramming factors and differentiated these hiPSCs into clinically relevant cell derivatives. In addition, to maintain clinical relevance, we implemented a methodology to clean our hiPSCs from non-human antigens to allow for current good manufacturing practice conditions that could help set the standard for human clinical trials with our factor-free hiPSCs. The field of stem cell reprogramming has rapidly advanced, and a new technique involving mRNA based reprogramming was introduced that we found to be difficult to reproduce due to an innate immune response based degradation of mRNA when introduced into the cell. To solve this problem, a small chemical compound was utilized that blocked important aspects of the innate immune response to single stranded mRNA that yielded robust and uniform expression of a key reprogramming factor. This stabilization could be important in increasing mRNA based reprogramming efficiency of hiPSC derivation. Another challenge in the hiPSC field is investigating nuanced potential differences manifested in transcriptional, epigenetic, immunological, and differentiation potentials between hESCs and hiPSCs. To help and potentially solve this problem and allow for more complete and faithful reprogramming to a hESC state, global microarray transcriptional analysis of oocyte cytoplasm was utilized to find eight putative novel shared reprogramming factors across multiple species. These factors have identifiable roles in opening up chromatin that can allow reprogramming factors to better access reprogramming loci that could confer the known reprogramming advantage that somatic cell nuclear transfer based reprogramming maintains over current direct reprogramming approaches. To address the recently observed immunogenicity issues of iPSCs, we studied the expression of two normally fetally associated genes implicated in an iPSC-specific immune response. We found high line-to-line variation between both hESC and hiPSC lines across different levels of differentiation and confirmed that current differentiation protocols derive cell types with a fetal phenotype as opposed to the adult phenotype needed for clinical applications as indicated by aberrant expression of specific fetal genes. Taken altogether, we hope these studies allow for more robust, reproducible, and clinically relevant hiPSCs that more closely resemble hESCs and maintain…

Subjects/Keywords: Pharmacology; Clinical Grade; Human Induced Pluripotent Stem Cells; Immunogenicity; Reprogramming; Stem Cell

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Awe, J. P. (2014). Investigating the Mechanisms of Reprogramming and Optimizing the Generation of Potentially Therapeutically Useful Induced Pluripotent Stem Cell Derivatives. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/25v79301

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Awe, Jason Patrick. “Investigating the Mechanisms of Reprogramming and Optimizing the Generation of Potentially Therapeutically Useful Induced Pluripotent Stem Cell Derivatives.” 2014. Thesis, UCLA. Accessed January 20, 2021. http://www.escholarship.org/uc/item/25v79301.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Awe, Jason Patrick. “Investigating the Mechanisms of Reprogramming and Optimizing the Generation of Potentially Therapeutically Useful Induced Pluripotent Stem Cell Derivatives.” 2014. Web. 20 Jan 2021.

Vancouver:

Awe JP. Investigating the Mechanisms of Reprogramming and Optimizing the Generation of Potentially Therapeutically Useful Induced Pluripotent Stem Cell Derivatives. [Internet] [Thesis]. UCLA; 2014. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/25v79301.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Awe JP. Investigating the Mechanisms of Reprogramming and Optimizing the Generation of Potentially Therapeutically Useful Induced Pluripotent Stem Cell Derivatives. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/25v79301

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

9. Schinzel, Robert. Die Kultur und Differenzierung von Humanen Embryonalen Stammzellen in Braune und Weisse Adipozyten.

Degree: 2012, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-14513

► Humane pluripotente Stammzellen (hPSC) haben das Potential die biologische und medizinische Forschung zu revolutionieren. Zuvor müssen jedoch eine Reihe grundlegender Probleme gelöst werden. So sind… (more)

▼ Humane pluripotente Stammzellen (hPSC) haben das Potential die biologische und medizinische Forschung zu revolutionieren. Zuvor müssen jedoch eine Reihe grundlegender Probleme gelöst werden. So sind die vorhandenen Kulturbedingungen ineffizient, unzuverlässig und aufwendig. Für viele wichtige Fragestellungen fehlen zudem geeignete Differenzierungsprotokolle, wodurch wichtige Zelltypen für Experimente nicht zur Verfügung stehen. Beispielsweise mangelt es an effektiven und robusten Strategien um Adipozyten in Zellkulturen herzustellen und zu kultivieren. Fettleibigkeit hat in den letzten Jahrzehnten epidemische Ausmaße in weiten Teilen der Bevölkerung erreicht, mit negativen Auswirkungen auf Gesundheit und Lebensqualität vieler Betroffenen. Ein wichtiger Faktor, für die mit Übergewicht assoziierten Stoffwechselerkrankungen, ist der Zustand von Adipozyten. Eine Methode die es ermöglicht funktionelle Adipozyten effizient in vitro zu kultivieren, würde die Untersuchung dieser Zellen im gesunden und pathologischen Zustand ermöglichen und könnte dadurch den Kampf gegen die Folgen der Fettleibigkeit unterstützen. Diese Doktorarbeit besteht aus zwei Teilen; der erste Teil bezieht sich auf die Entwicklung eines verbesserten Kultursystems von hPSCs, während der zweite Teil eine Methode zur Generierung von weißen und braunen Adipozyten aus Stammzellen vorstellt. Im ersten Teil der Arbeit demonstrieren wir, dass die sogenannte “enhanced culture platform” (ECP) geeignet für die Kultur von humanen Stammzellen ist. Zudem ermöglicht die Nutzung dieser Plattform Unzulänglichkeiten vorhandender Zellkultursysteme erheblich zu verbessern. Zum Beispiel wurden durch Optimierung der Kulturbedingungen, die Zellkultur sowohl effizienter gemacht und stabilisiert, als auch genetische Manipulation deutlich vereinfacht. Der zweite Teil der Arbeit hat die Entwicklung eines experimentellen Systems zur Differenzierung von hPSC in braune und weiße Adipozyten zum Thema. Dafür entwickelten wir eine Strategie die Zellen, mittels Überexpression ausgewählter Transkriptionsfaktoren, in bestimmte Zelltypen lenkt. Anhand einer Reihe von Untersuchungen auf morphologischer und molekularer Ebene folgerten wir, dass die Überexpression des adipozyten-spezifischen Transkriptionsfaktors PPARG2 ausreicht, um aus hPSC hergestellten mesodermalen Stammzellen effizient und robust vollständig ausgereifte weiße Fettzellen zu differenzieren. Die so generierten Adipozyten besitzen zudem für Fettzellen spezifische funktionelle Fähigkeiten: Sie können Lipolyse von Fettsäuren durchführen, neue Fettsäuren herstellen, Glukose nach Insulin-Stimulus aufnehmen und Adipokine freisetzen. Zusammenfassend bestätigten diese Ergebnisse die Identität und Funktionalität der Zellen und wir folgerten, dass wir erfolgreich ein in vitro System zur Herstellung von weißen Adipozyten entwickelt haben. Diese Resultate deuten zudem auf eine Eignung des Zellkultursystems für die Untersuchung des Einflusses von Adipozyten auf Krankheiten, wie zum Beispiel Fettleibigkeit, Lipodystrophie oder Diabetes… Advisors/Committee Members: [email protected] (contact), m (gender), Prof. Dr. Mutzel (firstReferee), Prof. Dr. Cowan (furtherReferee).

Subjects/Keywords: adipocytes; human embryonic stem cell; human induced pluripotent stem cells; differentiation; stem cells; programming; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schinzel, R. (2012). Die Kultur und Differenzierung von Humanen Embryonalen Stammzellen in Braune und Weisse Adipozyten. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-14513

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schinzel, Robert. “Die Kultur und Differenzierung von Humanen Embryonalen Stammzellen in Braune und Weisse Adipozyten.” 2012. Thesis, Freie Universität Berlin. Accessed January 20, 2021. http://dx.doi.org/10.17169/refubium-14513.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schinzel, Robert. “Die Kultur und Differenzierung von Humanen Embryonalen Stammzellen in Braune und Weisse Adipozyten.” 2012. Web. 20 Jan 2021.

Vancouver:

Schinzel R. Die Kultur und Differenzierung von Humanen Embryonalen Stammzellen in Braune und Weisse Adipozyten. [Internet] [Thesis]. Freie Universität Berlin; 2012. [cited 2021 Jan 20]. Available from: http://dx.doi.org/10.17169/refubium-14513.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schinzel R. Die Kultur und Differenzierung von Humanen Embryonalen Stammzellen in Braune und Weisse Adipozyten. [Thesis]. Freie Universität Berlin; 2012. Available from: http://dx.doi.org/10.17169/refubium-14513

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

10. Kolijn, K. The origin and future of hematopoietic stem cells.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/238606

► Hematopoietic stem cell (HSCs) therapy in the form of bone marrow transplantations has been used successfully in the clinic for over 40 years and continues… (more)

Subjects/Keywords: Hematopoietic stem cells; embryonic stem cells; induced pluripotent stem cells; ontogeny

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APA (6^th Edition):

Kolijn, K. (2012). The origin and future of hematopoietic stem cells. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/238606

Chicago Manual of Style (16^th Edition):

Kolijn, K. “The origin and future of hematopoietic stem cells.” 2012. Masters Thesis, Universiteit Utrecht. Accessed January 20, 2021. http://dspace.library.uu.nl:8080/handle/1874/238606.

MLA Handbook (7^th Edition):

Kolijn, K. “The origin and future of hematopoietic stem cells.” 2012. Web. 20 Jan 2021.

Vancouver:

Kolijn K. The origin and future of hematopoietic stem cells. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Jan 20]. Available from: http://dspace.library.uu.nl:8080/handle/1874/238606.

Council of Science Editors:

Kolijn K. The origin and future of hematopoietic stem cells. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/238606

Louisiana State University

11. Addison, Meredith Kathleen. Lentiviral transduction of epigenetically modified bovine adult stem cells.

Degree: MS, Animal Sciences, 2012, Louisiana State University

URL: etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024

► Bovine adipose-derived stem cells (ADS), a form of adult stem cells, are somatic cells that have similar characteristics of embryonic stem (ES) cells. Bovine ADS… (more)

▼ Bovine adipose-derived stem cells (ADS), a form of adult stem cells, are somatic cells that have similar characteristics of embryonic stem (ES) cells. Bovine ADS cells possess multipotent capabilities and have been found to express pluripotency genes associated with ES cells. The unique properties of ADS cells make them a desirable source for reprogramming experiments. The goal of reprogramming experiments is to transform somatic cells from a differentiated state to a pluripotent state. When somatic cells reprogram, there are certain epigenetic changes or modifications that must occur in order to successfully reprogram the nucleus. Epigenetic modifications will change the chromatin configuration without changing the DNA sequence. Somatic cells can be exposed to small molecules that may be able to reduce the chances of having incomplete chromatin modification. Two epigenetic modifying factors are a DNA methyltranferase inhibitor, zebularine (Zeb), and a histone deacetylase inhibitor, valproic acid (VPA). By inducing gene expression with the epigenetic modifiers, the cells may be stimulated to reprogram more efficiently than cells with lower gene expression. In the first experiment, three bovine ADS cell lines were treated with VPA or Zeb to observe the changes in expression levels of Oct4, Sox2, and Nanog (pluripotency-associated genes). The cells were treated for a period of 5, 7,10, or 14 days. VPA led to the highest increase of the pluripotency genes; however, both treatments may have produced a partial reprogramming. This partial reprogramming may result in the bovine ADS cells reaching complete pluripotency when combined with a reprogramming technique. In the second experiment, three bovine ADS cell lines were treated with VPA or Zeb for five days then followed with transduction using lentivirus. Oct4, Sox2, and Nanog were increased the highest when using epigenetic modifiers. Statistical differences for expression of the pluripotency-associated genes were found for cells treated with zebularine. While it was thought that viral transduction in combination with epigenetic modifiers would produce higher expression levels of the pluripotency-associated genes, this was not found to be true in this experiment.

Subjects/Keywords: bovine adult stem cells; induced pluripotent stem cells; stem cells

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APA (6^th Edition):

Addison, M. K. (2012). Lentiviral transduction of epigenetically modified bovine adult stem cells. (Masters Thesis). Louisiana State University. Retrieved from etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024

Chicago Manual of Style (16^th Edition):

Addison, Meredith Kathleen. “Lentiviral transduction of epigenetically modified bovine adult stem cells.” 2012. Masters Thesis, Louisiana State University. Accessed January 20, 2021. etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024.

MLA Handbook (7^th Edition):

Addison, Meredith Kathleen. “Lentiviral transduction of epigenetically modified bovine adult stem cells.” 2012. Web. 20 Jan 2021.

Vancouver:

Addison MK. Lentiviral transduction of epigenetically modified bovine adult stem cells. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2021 Jan 20]. Available from: etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024.

Council of Science Editors:

Addison MK. Lentiviral transduction of epigenetically modified bovine adult stem cells. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024

University of Melbourne

12. Viventi, Serena. Characterization of dorsal root ganglia sensory neurons from human pluripotent stem cells and their application for developing therapies to treat Friedreich Ataxia.

Degree: 2019, University of Melbourne

URL: http://hdl.handle.net/11343/227456

► Sensory neurons of the dorsal root ganglia (DRG) are the primary responders to stimuli inducing feelings of touch, pain, temperature, vibration, pressure and muscle tension.… (more)

▼ Sensory neurons of the dorsal root ganglia (DRG) are the primary responders to stimuli inducing feelings of touch, pain, temperature, vibration, pressure and muscle tension. They consist of multiple subpopulations based on their morphology, molecular and functional properties. Our understanding of DRG sensory neurons has been predominantly driven by rodent studies and using transformed cell lines, whereas less is known about human sensory DRG neurons simply because of limited availability of human tissue. Although these previous studies have been fundamental for our understanding of the sensory system, it is imperative to profile human DRG subpopulations as it is becoming evident that human sensory neurons do not share identical molecular and functional properties found in other species. Furthermore, there is a wide range of diseases and disorders that directly/indirectly cause sensory neuronal degeneration or dysfunctionality, such as Friedreich Ataxia (FRDA) disease. FRDA is an autosomal recessive disease characterized by degeneration of DRG proprioceptive sensory neurons, which is due to a low level of the mitochondrial protein Frataxin (FXN). Having an in vitro source of human DRG sensory neurons, such as from human pluripotent stem cells (hPSC), is paramount for studying their development, unique neuronal properties and for accelerating regenerative therapies to treat sensory neuropathies, including FRDA. To this end, in this thesis, we generated DRG sensory neurons both from hESC and induced pluripotent stem cells (iPSC) from FRDA patients, characterizing DRG sensory neurons subtypes. Our results show that our protocol gives rise to heterogenous subpopulations of sensory neurons, that include nociceptors, mechanoreceptors and proprioceptors, as indicated by immunostainings and Q-PCR analyses. We also observed that the generation of the subtypes is a dynamic process, with expression of different markers arising at different time points in cultures during differentiation. In summary, these data provide an in vitro platform to discover the unique gene expression profile of sensory neuronal cell types that may exist within the human DRG and offer also the possibility to use this platform to model sensory neurons-related diseases in vitro. Another aim of this thesis was to interrogate the ability of hPSC-derived sensory neurons to survive, differentiate and integrate in vivo. Transplantations of hPSC-derived sensory neural progenitors, both from hPSCs and FRDA iPSCs, were performed in the DRG of adult rats and tissue analyses were performed at 2-8 weeks post-transplantation. Our results showed survival of transplanted donor cells, within and surrounding the DRG and, most importantly, donor cells expressed markers of DRG sensory neurons and glia, demonstrating their capacity to differentiate to sensory neuronal and glial lineages in vivo. These novel studies start to address the possibility of developing cell replacement therapies for treating neurodegeneration occurring within the peripheral sensory nervous…

Subjects/Keywords: sensory neurons; human embryonic stem cells; induced pluripotent stem cells; Friedreich Ataxia; Dorsal Root Ganglia; stem cell therapy; gene-delivery; nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Viventi, S. (2019). Characterization of dorsal root ganglia sensory neurons from human pluripotent stem cells and their application for developing therapies to treat Friedreich Ataxia. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/227456

Chicago Manual of Style (16^th Edition):

Viventi, Serena. “Characterization of dorsal root ganglia sensory neurons from human pluripotent stem cells and their application for developing therapies to treat Friedreich Ataxia.” 2019. Doctoral Dissertation, University of Melbourne. Accessed January 20, 2021. http://hdl.handle.net/11343/227456.

MLA Handbook (7^th Edition):

Viventi, Serena. “Characterization of dorsal root ganglia sensory neurons from human pluripotent stem cells and their application for developing therapies to treat Friedreich Ataxia.” 2019. Web. 20 Jan 2021.

Vancouver:

Viventi S. Characterization of dorsal root ganglia sensory neurons from human pluripotent stem cells and their application for developing therapies to treat Friedreich Ataxia. [Internet] [Doctoral dissertation]. University of Melbourne; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11343/227456.

Council of Science Editors:

Viventi S. Characterization of dorsal root ganglia sensory neurons from human pluripotent stem cells and their application for developing therapies to treat Friedreich Ataxia. [Doctoral Dissertation]. University of Melbourne; 2019. Available from: http://hdl.handle.net/11343/227456

Universiteit Utrecht

13. Wever, I. From fibroblast to neuron: The use of stem cells for Parkinson's disease.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/258934

► One of the characterizations of Parkinson’s disease (PD) is the motor symptoms caused by the loss of the dopaminergic neurons of the substantia nigra pars… (more)

Subjects/Keywords: Parkinson's disease; Pluripotent Stem cells; Embryonic Stem cells; induced Pluripotent Stem cells; Neural development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wever, I. (2012). From fibroblast to neuron: The use of stem cells for Parkinson's disease. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/258934

Chicago Manual of Style (16^th Edition):

Wever, I. “From fibroblast to neuron: The use of stem cells for Parkinson's disease.” 2012. Masters Thesis, Universiteit Utrecht. Accessed January 20, 2021. http://dspace.library.uu.nl:8080/handle/1874/258934.

MLA Handbook (7^th Edition):

Wever, I. “From fibroblast to neuron: The use of stem cells for Parkinson's disease.” 2012. Web. 20 Jan 2021.

Vancouver:

Wever I. From fibroblast to neuron: The use of stem cells for Parkinson's disease. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Jan 20]. Available from: http://dspace.library.uu.nl:8080/handle/1874/258934.

Council of Science Editors:

Wever I. From fibroblast to neuron: The use of stem cells for Parkinson's disease. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/258934

California State University – Sacramento

14. Roenspie, Sean P. An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles.

Degree: MA, Biological Science (Stem Cell, 2011, California State University – Sacramento

URL: http://hdl.handle.net/10211.9/1093

► Fragile X-associated tremor/ataxia syndrome (FXTAS) is an adult onset neurodegenerative disorder affecting carriers of premutation expansions (55-200 CGG repeats) of the fragile X mental retardation… (more)

▼ Fragile X-associated tremor/ataxia syndrome (FXTAS) is an adult onset neurodegenerative disorder affecting carriers of premutation expansions (55-200 CGG repeats) of the fragile X mental retardation 1 (FMR1) gene. The clinical features of FXTAS, as well as various forms of clinical involvement in carriers without FXTAS, are thought to arise through a direct toxic gain-of-function of the FMR1 mRNA containing the expanded CGG repeat. A singular difficulty in the study of cellular regulation in FXTAS, as with most other neurogenetic disorders, is background genetic variation between/among cases and controls. To circumvent this problem, a novel approach was taken whereby single allele sub-clones were derived from a single individual who was profoundly mosaic for CGG repeat size, with CGG repeats in individual alleles ranging from the normal range (<50 CGG repeats) to well into the full mutation range (>700 CGG repeats). Sub-clones with expanded CGG repeat alleles, and those with CGG repeats in the normal range, thus represent background isogenic case-control comparison groups, since all clones derive from a single individual. In order to generate clonal fibroblast cell lines from the CGG repeat size mosaic, it was necessary to first develop a protocol that would successfully isolate single cells and allow for their efficient expansion. Once the protocol was established, a PCR-based method was used to genotype with respect to CGG repeat size each of the clonal cell lines. Through this process, fifteen clonal lines have been established to date, with representatives from normal (<55 CGG repeats), premutation (55-200 repeats) and full mutation (>200 repeats) ranges. These clonal lines are currently being characterized with respect to FMR1 gene expression (FMR1 mRNA and protein levels). The current clonal library forms the basis for our effort to reprogram the fibroblast cell lines into induced Pluripotent Stem Cells (iPSCs) with the intention of further differentiating the cells into neural progenitor cells, and subsequently, functional neurons. This research project was conducted in the department of Biochemistry and Molecular Medicine, University of California, Davis, School of Medicine, in the laboratory of Dr. Paul J Hagerman. Advisors/Committee Members: Peavy, Thomas R..

Subjects/Keywords: FXTAS; Neurodegenerative diseases; Induced pluripotent stem cells

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APA (6^th Edition):

Roenspie, S. P. (2011). An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles. (Masters Thesis). California State University – Sacramento. Retrieved from http://hdl.handle.net/10211.9/1093

Chicago Manual of Style (16^th Edition):

Roenspie, Sean P. “An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles.” 2011. Masters Thesis, California State University – Sacramento. Accessed January 20, 2021. http://hdl.handle.net/10211.9/1093.

MLA Handbook (7^th Edition):

Roenspie, Sean P. “An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles.” 2011. Web. 20 Jan 2021.

Vancouver:

Roenspie SP. An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles. [Internet] [Masters thesis]. California State University – Sacramento; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10211.9/1093.

Council of Science Editors:

Roenspie SP. An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles. [Masters Thesis]. California State University – Sacramento; 2011. Available from: http://hdl.handle.net/10211.9/1093

NSYSU

15. Wang, Yin-Hsuan. The cAMP/PKA-mediated Tau S409 phosphorylation through GSKIP/GSK3 axis in SH-SY5Y and iPS cells.

Degree: Master, Biological Sciences, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0404117-114837

► We previously described that PKA/GSKIP/GSK3Î² complex serves as a platform to anchor and phosphorylate Drp1 affecting mitochondria dynamics to provide neuroprotection. Recently, a known PKA/GSK3Î²… (more)

▼ We previously described that PKA/GSKIP/GSK3Î² complex serves as a platform to anchor and phosphorylate Drp1 affecting mitochondria dynamics to provide neuroprotection. Recently, a known PKA/GSK3Î² substrate, Tau, has been revealed its S409 phosphorylation is associating with the termination of neuron cells in Alzheimer disease. In this study, we attempt to extend that Tau phosphorylation is also mediated by PKA/GSKIP/GSK3Î² working complex. Our data showed that GSKIP-WT overexpression in SH-SY5Y cells increased phosphorylation of Tau S409 site (not S214; S262; S396; S404; T205 and T212 sites) over that of PKA- and GSK3Î² binding-defective mutants (V41/L45 and L130) under forskolin challenge, indicating that both PKA and GSK3Î² bindings may be associated to phosphorylate Tau at S409 site. Surprisingly, treatment with foskion in GSKIP-WT-overexpressing SH-SY5Y cells were significantly increase Tau phosphorylation at S409, suggesting that only PKA kinase activity, but not GSK3Î², is required in the GSKIP-mediated Tau phosphorylation. However, silencing of GSK3Î² resulted in a dramatic decrease in phosphorylation of Tau S409, revealing both GSKIP and GSK3Î² are crucial of PKA/GSKIP/GSK3Î²/Tau complex. Further, overexpressed kinase-dead GSK3Î² K85R (retains capacity to bind GSKIP) in SH-SY5Y cells, but not K85M (loss of capacity to bind GSKIP), has a higher Tau S409 phosphorylation, ensures that GSK3Î² acts solely as an anchor binding protein rather than its kinase activity in this signaling axis. Due to previous studies showed several different residues of Tau can be phosphorylated by PKA, we conducted In vitro kinase assay and provided two clear results: (1) As similar to early findings, PKA played a phosphorylation role on Ser409, Ser214 and Ser262 residues of Tau. (2) GSK3Î² provided a conformational shelter in PKA/GSKIP/GSK3Î²/Tau complex to harbor Tau Ser409 residue so that PKA is failed to phosphorylate Tau Ser409 residue. Furthermore, by using CRISPR/Cas9 system to produce APP WT/D678H and APP WT/WT ã APP D678H/D678H multifunctional stem cells (modified from an APP patient WT/D678H genotype), the results of analysis showed phosphorylation in Tau Ser262 and Ser214 residues and the Tau Ser409 intense phosphorylation in the brain of Alzheimerâs patients.. Coupling with previous findings of PKA suggested that the PKA/GSKIP/GSK3Î²/Tau complex may plays a key role on the development of Alzheimerâs disease. Taken together, our data provide compelling evidence to implicate that both GSKIP and GSK3Î² function as anchoring proteins to enhance cAMP/PKA/Tau axis signaling during Alzheimer pathogenesis. Advisors/Committee Members: Cheng Jiin-Tsuey (chair), Hong Yi-Ren (committee member), Huang Chi-Ying (chair), Chiou Shean-jaw (chair).

Subjects/Keywords: PKA; Tau; Alzheimer disease; Human induced pluripotent stem (iPS) cells; AÎ² precursor protein (; GSK3Î²; GSKIP

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APA (6^th Edition):

Wang, Y. (2017). The cAMP/PKA-mediated Tau S409 phosphorylation through GSKIP/GSK3 axis in SH-SY5Y and iPS cells. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0404117-114837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Yin-Hsuan. “The cAMP/PKA-mediated Tau S409 phosphorylation through GSKIP/GSK3 axis in SH-SY5Y and iPS cells.” 2017. Thesis, NSYSU. Accessed January 20, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0404117-114837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Yin-Hsuan. “The cAMP/PKA-mediated Tau S409 phosphorylation through GSKIP/GSK3 axis in SH-SY5Y and iPS cells.” 2017. Web. 20 Jan 2021.

Vancouver:

Wang Y. The cAMP/PKA-mediated Tau S409 phosphorylation through GSKIP/GSK3 axis in SH-SY5Y and iPS cells. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 20]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0404117-114837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang Y. The cAMP/PKA-mediated Tau S409 phosphorylation through GSKIP/GSK3 axis in SH-SY5Y and iPS cells. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0404117-114837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

16. Abud, Edsel Misael. Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases.

Degree: Biological Sciences, 2017, University of California – Irvine

URL: http://www.escholarship.org/uc/item/12b1q5fv

► Microglia play an important role in developmental and homeostatic brain function. They profoundly influence the development and progression of neurological disorders, including Alzheimer’s disease (AD).… (more)

▼ Microglia play an important role in developmental and homeostatic brain function. They profoundly influence the development and progression of neurological disorders, including Alzheimer’s disease (AD). In AD, microglia respond to degenerating neurons, beta-amyloid (Aβ) and tau pathology, and CNS inflammation. AD-etiology involves complex interactions between age, genetic, and environmental risk factors. Recently, GWAS studies in large AD cohorts have identified numerous SNPs present in innate immune genes, many expressed in microglia. These SNPs confer an elevated risk in developing AD, and harboring of these SNPS is considered a risk factor. While the genes themselves are not risk factors, SNPs in these genes may impair the normal function of microglia, such as cytokine production and phagocytosis. Furthermore, many of these genes are involved in the detection or clearance of Aβ, and therefore these SNPs may collectively influence microglia function or influence dysfunction.Studying human microglia is challenging because of the rarity and difficulty in acquiring cells from human CNS tissue. A methodology for generating human microglia from a renewable source has long remained elusive despite success in generating the other cell-types of the CNS. This elusiveness existed due to the ontogeny of microglia and their unique transcriptome and functional differences versus other myeloid cells. The goal of my dissertation was to generate human induced pluripotent stem cell (iPSC)-derived microglia (iMGLs) to study their molecular and physiological function in neurological disease. To accomplish this goal, I developed a fully-defined protocol to generate large number of pure (>95%) iMGLs under 40 days. iMGLs are highly like primary human microglia as assessed via whole-transcriptome and functional analyses. iMGL studies, in vitro and in vivo, highly suggest that they can be used as surrogates for human p microglia. Using iMGLs, I showed how AD-GWAS related microglial genes were influenced by two hallmark AD proteins—Aβ and Tau. In addition, I showed that iMGLs populate mouse brains and develop microglial-like arborized morphology and respond to Aβ in an AD transgenic mice. Together, this protocol represents an important advance in our ability to study human microglia in the context of development, health, and disease, such as AD

Subjects/Keywords: Neurosciences; Immunology; alzheimer's disease; disease modeling; human-mouse transplantation; induced pluripotent stem cells; microglia

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APA (6^th Edition):

Abud, E. M. (2017). Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/12b1q5fv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abud, Edsel Misael. “Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases.” 2017. Thesis, University of California – Irvine. Accessed January 20, 2021. http://www.escholarship.org/uc/item/12b1q5fv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abud, Edsel Misael. “Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases.” 2017. Web. 20 Jan 2021.

Vancouver:

Abud EM. Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases. [Internet] [Thesis]. University of California – Irvine; 2017. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/12b1q5fv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abud EM. Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases. [Thesis]. University of California – Irvine; 2017. Available from: http://www.escholarship.org/uc/item/12b1q5fv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

17. Kumar, Kevin Krishan. Investigation of Neuronal Manganese Regulation in Physiology and Disease Using High Throughput Screening, Induced Pluripotent Stem Cells, and Chemical Biology Approaches.

Degree: PhD, Neuroscience, 2014, Vanderbilt University

URL: http://hdl.handle.net/1803/14021

► Manganese (Mn) is both an essential biological cofactor and neurotoxicant. Disruption of Mn biology in the basal ganglia has been implicated in the pathogenesis of… (more)

Subjects/Keywords: High Throughput Screening; Manganese; Neurodegenerative diseases; Human Induced Pluripotent Stem Cells; Metabolomics

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APA (6^th Edition):

Kumar, K. K. (2014). Investigation of Neuronal Manganese Regulation in Physiology and Disease Using High Throughput Screening, Induced Pluripotent Stem Cells, and Chemical Biology Approaches. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14021

Chicago Manual of Style (16^th Edition):

Kumar, Kevin Krishan. “Investigation of Neuronal Manganese Regulation in Physiology and Disease Using High Throughput Screening, Induced Pluripotent Stem Cells, and Chemical Biology Approaches.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 20, 2021. http://hdl.handle.net/1803/14021.

MLA Handbook (7^th Edition):

Kumar, Kevin Krishan. “Investigation of Neuronal Manganese Regulation in Physiology and Disease Using High Throughput Screening, Induced Pluripotent Stem Cells, and Chemical Biology Approaches.” 2014. Web. 20 Jan 2021.

Vancouver:

Kumar KK. Investigation of Neuronal Manganese Regulation in Physiology and Disease Using High Throughput Screening, Induced Pluripotent Stem Cells, and Chemical Biology Approaches. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1803/14021.

Council of Science Editors:

Kumar KK. Investigation of Neuronal Manganese Regulation in Physiology and Disease Using High Throughput Screening, Induced Pluripotent Stem Cells, and Chemical Biology Approaches. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/14021

University of Edinburgh

18. Zhao, Chen. Investigation of the cell- and non-cell autonomous impact of the C9orf72 mutation on human induced pluripotent stem cell-derived astrocytes.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/25903

► Amyotrophic lateral sclerosis (ALS) is a late onset neurodegenerative disorder characterised by selective loss of upper and lower motor neurons (MNs). Recently, the GGGGCC (G4C2)… (more)

Subjects/Keywords: 616.8; ALS; amyotrophic lateral sclerosis; astrocytes; human induced pluripotent stem cells; iPSCs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, C. (2016). Investigation of the cell- and non-cell autonomous impact of the C9orf72 mutation on human induced pluripotent stem cell-derived astrocytes. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/25903

Chicago Manual of Style (16^th Edition):

Zhao, Chen. “Investigation of the cell- and non-cell autonomous impact of the C9orf72 mutation on human induced pluripotent stem cell-derived astrocytes.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed January 20, 2021. http://hdl.handle.net/1842/25903.

MLA Handbook (7^th Edition):

Zhao, Chen. “Investigation of the cell- and non-cell autonomous impact of the C9orf72 mutation on human induced pluripotent stem cell-derived astrocytes.” 2016. Web. 20 Jan 2021.

Vancouver:

Zhao C. Investigation of the cell- and non-cell autonomous impact of the C9orf72 mutation on human induced pluripotent stem cell-derived astrocytes. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1842/25903.

Council of Science Editors:

Zhao C. Investigation of the cell- and non-cell autonomous impact of the C9orf72 mutation on human induced pluripotent stem cell-derived astrocytes. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/25903

University of New South Wales

19. Fonoudi, Hananeh. Human Induced Pluripotent Stem Cells as a Model of Complex Cardiac Disorders.

Degree: Victor Chang Cardiac Research Institute, 2018, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/60401 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:52016/SOURCE02?view=true

► Hypoplastic left heart (HLH) is a genetically complex disease, characterized by hypoplasia of the left side of the heart. Although it is one of the… (more)

▼ Hypoplastic left heart (HLH) is a genetically complex disease, characterized by hypoplasia of the left side of the heart. Although it is one of the most severe forms of congenital heart defects, our current knowledge of the molecular underpinnings of the disease is very limited. Here, we have generated an in vitro model of HLH using human induced pluripotent stem cells (hiPSCs) to uncover disease-causing factors. hiPSCs were generated from 10 unrelated HLH patients and their non-HLH parents (trio design; 3 clones per individual; 87 hiPSC lines in total), providing controls that are as genetically similar to the patients as possible. To investigate differences during early stages of cardiovascular development, hiPSCs were differentiated using both embryoid body and small molecule cardiac-directed differentiation methods. Cellular populations produced upon differentiation and their respective gene expression profiles were studied. Gene expression analysis of the spontaneously differentiated cells showed lower expression of both cardiac and vascular smooth muscle markers in patients compared to controls. Flow cytometry analysis performed on hiPSC cultures after directed cardiac differentiation at 5-day intervals (days 0-30) showed ventricular cardiomyocyte differentiation in HLH-hiPSCs was perturbed. A time-course analysis on 5 HLH families using RNAseq revealed that the greatest differences between patients and parents was at day 20 post-differentiation initiation, with down-regulation of cell cycle related pathways being the main driver. This finding was further validated using a second independent cohort of 5 HLH families. Proliferation assays corroborated our RNAseq data and showed an approximate 50% reduction in the percentage of dividing cardiomyocytes derived from HLH-hiPSCs compared to parents (P < 0.05). Cell phenotyping also indicated that beating cardiomyocytes derived from patients were more immature and their calcium flux properties were significantly different (n > 1000; P < 0.001). In summary, our findings thus far suggest that the progression of cardiogenesis and vasculogenesis in HLH-hiPSCs is perturbed, which may be based on differences in cell cycle properties. Furthermore, the functionality of cardiomyocytes derived from HLH-hiPSCs was altered (with respect to calcium flux properties), suggestive of cardiomyocyte immaturity. Our data suggest a common pathogenic pathway underlying the formation of HLH despite the genetic heterogeneity of disease causation. Advisors/Committee Members: Harvey, Richard, Victor Chang Cardiac Research Institute, Faculty of Medicine, UNSW.

Subjects/Keywords: Cardiomyocytes; Human induced pluripotent stem cells; Hypoplastic left heart; Congenital heart defect

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fonoudi, H. (2018). Human Induced Pluripotent Stem Cells as a Model of Complex Cardiac Disorders. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/60401 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:52016/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Fonoudi, Hananeh. “Human Induced Pluripotent Stem Cells as a Model of Complex Cardiac Disorders.” 2018. Doctoral Dissertation, University of New South Wales. Accessed January 20, 2021. http://handle.unsw.edu.au/1959.4/60401 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:52016/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Fonoudi, Hananeh. “Human Induced Pluripotent Stem Cells as a Model of Complex Cardiac Disorders.” 2018. Web. 20 Jan 2021.

Vancouver:

Fonoudi H. Human Induced Pluripotent Stem Cells as a Model of Complex Cardiac Disorders. [Internet] [Doctoral dissertation]. University of New South Wales; 2018. [cited 2021 Jan 20]. Available from: http://handle.unsw.edu.au/1959.4/60401 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:52016/SOURCE02?view=true.

Council of Science Editors:

Fonoudi H. Human Induced Pluripotent Stem Cells as a Model of Complex Cardiac Disorders. [Doctoral Dissertation]. University of New South Wales; 2018. Available from: http://handle.unsw.edu.au/1959.4/60401 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:52016/SOURCE02?view=true

Freie Universität Berlin

20. Metzler, Eric. Generierung von hiPSCs und Redifferenzierung in induzierte myogene Zellen - abhängig vom Ursprungszelltyp?.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-27214

► Muskeldystrophien sind eine heterogene Gruppe von erblichen Muskelerkrankungen, die durch progressive Muskeldegeneration charakterisiert sind. Zellersatztherapien zielen darauf ab die geschädigten Muskelzellen zu ersetzten und den… (more)

▼ Muskeldystrophien sind eine heterogene Gruppe von erblichen Muskelerkrankungen, die durch progressive Muskeldegeneration charakterisiert sind. Zellersatztherapien zielen darauf ab die geschädigten Muskelzellen zu ersetzten und den degenerierten Muskel zu regenerieren und gelten als vielversprechende therapeutische Strategie zur Behandlung von Patienten mit Muskeldystrophien. Die Regeneration von Muskelgewebe hängt von muskelspezifischen Stammzellen ab, den sogenannten Satellitenzellen. Satellitenzellen kommen jedoch nur in sehr geringer Anzahl vor und sind daher nicht in ausreichendem Maße für eine ex vivo Kultivierung und genetische Korrektur vorhanden. Humane induzierte pluripotente Stammzellen (hiPSCs) hingegen, stellen den Schlüssel zu einer uneingeschränkten Anzahl autologer Zellen dar, die zur genetischen Korrektur und zur Regeneration von großen Muskeln benötigt werden, ohne dass es dabei zu Immunabstoßungsreaktionen kommt. hiPSCs wurden bereits aus diversen Zelltypen generiert. Zudem wurden bereits diverse Protokolle zur Generierung von Muskelzellen oder auch Muskelstammzellen aus hiPSCs etabliert. Dennoch ist das biotechnologische und therapeutische Potential dieser aus hiPSCs induzierten Muskelzellen bisher unklar. Auch der Einfluss des somatischen Ursprungszelltyps der hiPSCs auf ihre Kapazität in Muskelzellen zu differenzieren ist nicht abschließend geklärt. Myoblasten und periphere mononukleäre Blutzellen (PBMCs) stellen die beiden Zelltypen mit der größten Relevanz für die Generierung von autologen hiPSCs zur Behandlung von Muskeldystrophien dar. Blutproben sind leicht zu entnehmen und Myoblasten können aus Muskelbiopsie-Proben isolierten werden, die zu diagnostischen Zwecken von Patienten mit Verdacht auf Muskelerkrankungen entnommen werden. Daher wurden in dieser Arbeit humane Myoblasten und PBMCs vom selben Donor isoliert (n=4) und deren Kapazität zur Reprogrammierung in hiPSCs untersucht. Zudem wurden die generierten hiPSCs auf transkriptioneller Ebene verglichen und, unter Verwendung eines nicht-integrativen myogenen Differenzierungsprotokolls, ihre Kapazität zur in vitro Differenzierung in die myogene Linie bestimmt. Im Anschluss wurde das Potential dieser aus hiPSCs induzierten Muskelzellen zur Bildung von humanen Muskelfasern in einem immunsupprimierten Mausmodell analysiert. Die Reprogrammierung in hiPSCs war mit Myoblasten deutlich effizienter als mit PBMCs. Der Vergleich der generierten hiPSCs auf transkriptioneller Ebene ergab 122 signifikant unterschiedlich exprimierte Transkripte zwischen den beiden Zelltypen. Diese Transkripte sind in Signalwege mit potentiellem Einfluss auf die Differenzierungskapazitäten dieser pluripotenten Stammzellen involviert. Dennoch zeigten die in vitro Differenzierungsexperimente keine signifikanten Unterschiede in der Anzahl an generierten Muskelzellen zwischen den aus Myoblasten und PBMCs generiertenhiPSCs vom selben Donor. Die Differenzierungskapazität der hiPSCs aus verschiedenen Donoren unterschied sich allerdings stark voneinander, was den… Advisors/Committee Members: male (gender), Spuler, Simone (firstReferee), Stricker, Sigmar (furtherReferee).

Subjects/Keywords: Myogenic differentiation; human induced Pluripotent Stem Cells (hiPSCs); Epigenetic memory; Reprogramming; ddc:572

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Metzler, E. (2020). Generierung von hiPSCs und Redifferenzierung in induzierte myogene Zellen - abhängig vom Ursprungszelltyp?. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27214

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Metzler, Eric. “Generierung von hiPSCs und Redifferenzierung in induzierte myogene Zellen - abhängig vom Ursprungszelltyp?.” 2020. Thesis, Freie Universität Berlin. Accessed January 20, 2021. http://dx.doi.org/10.17169/refubium-27214.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Metzler, Eric. “Generierung von hiPSCs und Redifferenzierung in induzierte myogene Zellen - abhängig vom Ursprungszelltyp?.” 2020. Web. 20 Jan 2021.

Vancouver:

Metzler E. Generierung von hiPSCs und Redifferenzierung in induzierte myogene Zellen - abhängig vom Ursprungszelltyp?. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Jan 20]. Available from: http://dx.doi.org/10.17169/refubium-27214.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Metzler E. Generierung von hiPSCs und Redifferenzierung in induzierte myogene Zellen - abhängig vom Ursprungszelltyp?. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27214

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Laurentian University

21. Dénommé, Ginny Michelle. Induced pluripotent stem cells: where are we today? .

Degree: 2015, Laurentian University

URL: https://zone.biblio.laurentian.ca/dspace/handle/10219/2491

► For many years, scientists have been trying to elucidate the molecular mechanisms that convey pluripotency and self-renewal to stem cells. With this valuable knowledge, they… (more)

Subjects/Keywords: stem cells; somatic cells; induced pluripotent stem cells; self-renewal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dénommé, G. M. (2015). Induced pluripotent stem cells: where are we today? . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/dspace/handle/10219/2491

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dénommé, Ginny Michelle. “Induced pluripotent stem cells: where are we today? .” 2015. Thesis, Laurentian University. Accessed January 20, 2021. https://zone.biblio.laurentian.ca/dspace/handle/10219/2491.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dénommé, Ginny Michelle. “Induced pluripotent stem cells: where are we today? .” 2015. Web. 20 Jan 2021.

Vancouver:

Dénommé GM. Induced pluripotent stem cells: where are we today? . [Internet] [Thesis]. Laurentian University; 2015. [cited 2021 Jan 20]. Available from: https://zone.biblio.laurentian.ca/dspace/handle/10219/2491.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dénommé GM. Induced pluripotent stem cells: where are we today? . [Thesis]. Laurentian University; 2015. Available from: https://zone.biblio.laurentian.ca/dspace/handle/10219/2491

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Michigan

22. Qian, Xu. Defining the Mechanism by which Synthetic Polymer Surfaces Support Human Pluripotent Stem Cell Self-Renewal.

Degree: PhD, Oral Health Sciences, 2015, University of Michigan

URL: http://hdl.handle.net/2027.42/113368

► Human pluripotent stem cells (hPSCs), which include embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have become a promising resource for regenerative medicine… (more)

▼ Human pluripotent stem cells (hPSCs), which include embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have become a promising resource for regenerative medicine and research into early development because these cells are able to indefinitely self-renew and are capable of differentiation into specialized cell types of all three germ layers and trophoectoderm. However, a major limitation for successful therapeutic application of hPSCs and their derivatives is the potential xenogeneic contamination and instability of current culture conditions. Synthetic polymers, such as poly[2-(methacryloyloxy) ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide] (PMEDSAH), offer multiple advantages over mouse embryonic fibroblasts (MEFs) and Matrigel for hPSC culture. The main purpose of this dissertation is to define the mechanisms by which hPSCs are propagated on synthetic polymers. By physical modifications of PMEDSAH, we found that modifying substrate thickness changed the physical properties, and thus altered pluripotent stem cell behavior. Our data suggest that the 105 nm thick atom transfer radical polymerization (ATRP) PMEDSAH possesses the optimal gel architecture for hPSC expansion with its intermediate thickness, hydrophilicity, surface charge, and a moderate degree of inter-chain association. Our findings demonstrate the importance of polymer physical properties in hPSC expansion. The 105 nm thick ATRP PMEDSAH and similar modifications may be used to obtain scalable populations of clinical-grade hPSCs for regenerative medicine. Although a specific group of transcription factors, such as OCT4, SOX2 and NANOG, are known to play critical roles in hPSC pluripotency and reprogramming, other factors and the key signaling pathways regulating these important properties are not completely understood. In this dissertation, we also investigated the role of the PSC marker Developmental Pluripotency Associated 5 (DPPA5) in hPSCs. Our data demonstrate higher expression of DPPA5 in hPSCs under PMEDSAH and other feeder-free conditions, compared to MEFs. DPPA5 stabilizes protein levels and enhances the function of NANOG. Finally, DPPA5 increases the hiPSC-reprogramming efficiency. These results provide new molecular insight into the function of the DPPA5 in hPSCs. Our findings extend our understanding of the mechanism by which PMEDSAH and other feeder-free conditions support hPSC self-renewal, and offers improvements to current protocols in hPSC maintenance and reprogramming. Advisors/Committee Members: Krebsbach, Paul H. (committee member), Lahann, Joerg (committee member), Kapila, Yvonne (committee member), Taichman, Russell S. (committee member).

Subjects/Keywords: human embryonic stem cells; induced pluripotent stem cells; synthetic polymers; Self-renewal; DPPA5; gel architecture; Biomedical Engineering; Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qian, X. (2015). Defining the Mechanism by which Synthetic Polymer Surfaces Support Human Pluripotent Stem Cell Self-Renewal. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/113368

Chicago Manual of Style (16^th Edition):

Qian, Xu. “Defining the Mechanism by which Synthetic Polymer Surfaces Support Human Pluripotent Stem Cell Self-Renewal.” 2015. Doctoral Dissertation, University of Michigan. Accessed January 20, 2021. http://hdl.handle.net/2027.42/113368.

MLA Handbook (7^th Edition):

Qian, Xu. “Defining the Mechanism by which Synthetic Polymer Surfaces Support Human Pluripotent Stem Cell Self-Renewal.” 2015. Web. 20 Jan 2021.

Vancouver:

Qian X. Defining the Mechanism by which Synthetic Polymer Surfaces Support Human Pluripotent Stem Cell Self-Renewal. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2027.42/113368.

Council of Science Editors:

Qian X. Defining the Mechanism by which Synthetic Polymer Surfaces Support Human Pluripotent Stem Cell Self-Renewal. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/113368

University of Edinburgh

23. Ríos De Anda, María Elena Mitzy. Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC.

Degree: PhD, 2020, University of Edinburgh

URL: http://hdl.handle.net/1842/36895

► Cellular reprogramming to pluripotency can be achieved by using a defined cocktail of transcription factors, converting differentiated somatic cells into induced pluripotent stem cells (iPSCs).… (more)

▼ Cellular reprogramming to pluripotency can be achieved by using a defined cocktail of transcription factors, converting differentiated somatic cells into induced pluripotent stem cells (iPSCs). These cells behave like embryonic stem cells (ES) and can be used to generate all cell types in the body. OCT4, SOX2, KLF4 and cMYC (OSKM) were the first set of reprogramming factors defined based on both their importance on maintaining pluripotency, and their ability to reprogram mouse and human somatic cells to iPSCs. Despite recent progress in refining the reprogramming technique, this process is still highly inefficient and often leads to partially reprogrammed cells. This is even more dramatic in human cells, where efficiency is low (∼0.001–1%) hindering the therapeutic prospects and promise of iPSCs and their clinical applications. Extensive studies have been focused in elucidating the molecular mechanisms by which the transcription factors contribute to reprogramming, but there is still a notable lack of understanding of the reprogramming process at the protein level. Particularly little is known about the protein network by which the reprogramming factors maintain pluripotency in human ES and drive pluripotency during the reprogramming process. Due to the relevance of OCT4 as a core transcription factor for both, the pluripotency network and the reprogramming process, the work present herein focused on studying OCT4 at the proteomic level. By exploiting proteomic approaches focused in on and off-chromatin bound proteins this work described for the first time the OCT4 protein interactors involved in early stages of reprogramming and during pluripotency. For the hES interactome, OCT4 binding partners involved in pluripotency maintenance were identified, in addition to a new set of chromatin associated proteins that have non-previously been described in the pluripotency context. On the other hand, during early reprogramming, OCT4 interactors included somatic transcription factors and other interacting proteins non-previously reported in the reprogramming context, such as proteins involved in cell death, development and differentiation. Further comparison of both interactomes revealed that the initial engagement of OCT4 with the somatic proteome is markedly different from that in ES, illustrating how OCT4 is able to change its chromatin-binding dynamics in order to stablish different phenotypes. Additionally, the analysis was expanded by applying the same on and off chromatin proteomic approaches to three OCT4 mutants bearing deletions of essential or non-essential reprogramming regions located in the transactivation domains (TAD). This allowed the identification of a unique set of seven proteins present in all the OCT4 with reprogramming capacity (WT or mutant). Interestingly, these were not associated with OCT4 in ES nor the deficient OCT4 mutant bearing a deletion of an essential reprogramming domain. This set of proteins included: UFD1L, RAI1, TNIP2, ETV4, XPO6, FBRSL1 and MCMBP and further functional analysis proved…

Subjects/Keywords: embryonic stem cells; induced pluripotent stem cells; iPSC; OCT4; SOX2; KLF4; cMYC; somatic transcription factors; proteomic approach analysis; human iPSCs reprogramming

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ríos De Anda, M. E. M. (2020). Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/36895

Chicago Manual of Style (16^th Edition):

Ríos De Anda, María Elena Mitzy. “Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC.” 2020. Doctoral Dissertation, University of Edinburgh. Accessed January 20, 2021. http://hdl.handle.net/1842/36895.

MLA Handbook (7^th Edition):

Ríos De Anda, María Elena Mitzy. “Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC.” 2020. Web. 20 Jan 2021.

Vancouver:

Ríos De Anda MEM. Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC. [Internet] [Doctoral dissertation]. University of Edinburgh; 2020. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1842/36895.

Council of Science Editors:

Ríos De Anda MEM. Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC. [Doctoral Dissertation]. University of Edinburgh; 2020. Available from: http://hdl.handle.net/1842/36895

University of Edinburgh

24. Ríos De Anda, María Elena Mitzy. Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC.

Degree: PhD, 2020, University of Edinburgh

URL: https://doi.org/10.7488/era/196 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.802307

► Cellular reprogramming to pluripotency can be achieved by using a defined cocktail of transcription factors, converting differentiated somatic cells into induced pluripotent stem cells (iPSCs).… (more)

▼ Cellular reprogramming to pluripotency can be achieved by using a defined cocktail of transcription factors, converting differentiated somatic cells into induced pluripotent stem cells (iPSCs). These cells behave like embryonic stem cells (ES) and can be used to generate all cell types in the body. OCT4, SOX2, KLF4 and cMYC (OSKM) were the first set of reprogramming factors defined based on both their importance on maintaining pluripotency, and their ability to reprogram mouse and human somatic cells to iPSCs. Despite recent progress in refining the reprogramming technique, this process is still highly inefficient and often leads to partially reprogrammed cells. This is even more dramatic in human cells, where efficiency is low (∼0.001–1%) hindering the therapeutic prospects and promise of iPSCs and their clinical applications. Extensive studies have been focused in elucidating the molecular mechanisms by which the transcription factors contribute to reprogramming, but there is still a notable lack of understanding of the reprogramming process at the protein level. Particularly little is known about the protein network by which the reprogramming factors maintain pluripotency in human ES and drive pluripotency during the reprogramming process. Due to the relevance of OCT4 as a core transcription factor for both, the pluripotency network and the reprogramming process, the work present herein focused on studying OCT4 at the proteomic level. By exploiting proteomic approaches focused in on and off-chromatin bound proteins this work described for the first time the OCT4 protein interactors involved in early stages of reprogramming and during pluripotency. For the hES interactome, OCT4 binding partners involved in pluripotency maintenance were identified, in addition to a new set of chromatin associated proteins that have non-previously been described in the pluripotency context. On the other hand, during early reprogramming, OCT4 interactors included somatic transcription factors and other interacting proteins non-previously reported in the reprogramming context, such as proteins involved in cell death, development and differentiation. Further comparison of both interactomes revealed that the initial engagement of OCT4 with the somatic proteome is markedly different from that in ES, illustrating how OCT4 is able to change its chromatin-binding dynamics in order to stablish different phenotypes. Additionally, the analysis was expanded by applying the same on and off chromatin proteomic approaches to three OCT4 mutants bearing deletions of essential or non-essential reprogramming regions located in the transactivation domains (TAD). This allowed the identification of a unique set of seven proteins present in all the OCT4 with reprogramming capacity (WT or mutant). Interestingly, these were not associated with OCT4 in ES nor the deficient OCT4 mutant bearing a deletion of an essential reprogramming domain. This set of proteins included: UFD1L, RAI1, TNIP2, ETV4, XPO6, FBRSL1 and MCMBP and further functional analysis proved…

Subjects/Keywords: embryonic stem cells; induced pluripotent stem cells; iPSC; OCT4; SOX2; KLF4; cMYC; somatic transcription factors; proteomic approach analysis; human iPSCs reprogramming

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ríos De Anda, M. E. M. (2020). Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC. (Doctoral Dissertation). University of Edinburgh. Retrieved from https://doi.org/10.7488/era/196 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.802307

Chicago Manual of Style (16^th Edition):

Ríos De Anda, María Elena Mitzy. “Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC.” 2020. Doctoral Dissertation, University of Edinburgh. Accessed January 20, 2021. https://doi.org/10.7488/era/196 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.802307.

MLA Handbook (7^th Edition):

Ríos De Anda, María Elena Mitzy. “Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC.” 2020. Web. 20 Jan 2021.

Vancouver:

Ríos De Anda MEM. Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC. [Internet] [Doctoral dissertation]. University of Edinburgh; 2020. [cited 2021 Jan 20]. Available from: https://doi.org/10.7488/era/196 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.802307.

Council of Science Editors:

Ríos De Anda MEM. Initial OCT4 engagement with the somatic proteome during reprogramming to iPSC. [Doctoral Dissertation]. University of Edinburgh; 2020. Available from: https://doi.org/10.7488/era/196 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.802307

Universiteit Utrecht

25. Boer, A.S. de. iPS cell reprogramming.

Degree: 2009, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/35214

► Recently, pluripotent cells that are not derived from an embryo have been generated. These so called induced pluripotent stem cells (iPS cells) are pluripotent stem… (more)

Subjects/Keywords: Geneeskunde; embryonic stem cells, induced pluripotent stem cells, reprogramming, pluripotency, epigenetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boer, A. S. d. (2009). iPS cell reprogramming. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/35214

Chicago Manual of Style (16^th Edition):

Boer, A S de. “iPS cell reprogramming.” 2009. Masters Thesis, Universiteit Utrecht. Accessed January 20, 2021. http://dspace.library.uu.nl:8080/handle/1874/35214.

MLA Handbook (7^th Edition):

Boer, A S de. “iPS cell reprogramming.” 2009. Web. 20 Jan 2021.

Vancouver:

Boer ASd. iPS cell reprogramming. [Internet] [Masters thesis]. Universiteit Utrecht; 2009. [cited 2021 Jan 20]. Available from: http://dspace.library.uu.nl:8080/handle/1874/35214.

Council of Science Editors:

Boer ASd. iPS cell reprogramming. [Masters Thesis]. Universiteit Utrecht; 2009. Available from: http://dspace.library.uu.nl:8080/handle/1874/35214

26. 山添, 知宏. Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する.

Degree: 博士（医学）, 2015, Hamamatsu University School of Medicine / 浜松医科大学

URL: http://hdl.handle.net/10271/2879

►

Abstract. Although neural and mesenchymal stem cells have been well-known to have a strong glioma tropism, this activity in induced pluripotent stem cells (iPSCs) has… (more)

Subjects/Keywords: induced pluripotent stem cells; neural stem cells; glioma; migration; gene therapy

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APA (6^th Edition):

山添, �. (2015). Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する. (Thesis). Hamamatsu University School of Medicine / 浜松医科大学. Retrieved from http://hdl.handle.net/10271/2879

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

山添, 知宏. “Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する.” 2015. Thesis, Hamamatsu University School of Medicine / 浜松医科大学. Accessed January 20, 2021. http://hdl.handle.net/10271/2879.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

山添, 知宏. “Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する.” 2015. Web. 20 Jan 2021.

Vancouver:

山添 �. Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する. [Internet] [Thesis]. Hamamatsu University School of Medicine / 浜松医科大学; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10271/2879.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

山添 �. Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する. [Thesis]. Hamamatsu University School of Medicine / 浜松医科大学; 2015. Available from: http://hdl.handle.net/10271/2879

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

27. Schnabel, Lauren. Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells.

Degree: PhD, Veterinary Medicine, 2013, Cornell University

URL: http://hdl.handle.net/1813/34305

► Advancement of stem cell therapy is dependent upon the practicality, safety, and efficacy of the cells being evaluated for clinical application. Over the past decade,… (more)

▼ Advancement of stem cell therapy is dependent upon the practicality, safety, and efficacy of the cells being evaluated for clinical application. Over the past decade, the need for banked stem cells which are readily available for use at the time of a patient's diagnosis has become apparent. The overall goal of this dissertation research was to compare induced pluripotent stem cells (iPSCs) to bone marrow-derived mesenchymal stem cells (MSCs), first in terms of their ability to be generated from genetically diverse individuals, and then in terms of their immunogenic and immunomodulatory properties for potential allogeneic use. It has previously been demonstrated in mice that genetic background affects the proliferation and differentiation rates of MSCs. The purpose of our first study was to determine if genetic background affects the efficiency of generating iPSCs from mice. Results of this study confirmed that genetic background does affect both the efficiency of generating iPSCs during the early stages of reprogramming as well as the pluripotent stability of the iPSCs during later stages of reprogramming. The results also confirmed the need to understand the immunogenic and immunomodulatory properties of these cells for potential allogeneic application given that it may not be feasible to generate iPSCs from all individuals or to wait for the time that it takes to generate iPSCs and then screen them for safety and efficacy. The purpose of our second study, therefore, was to evaluate the in vitro immunogenic and immunomodulatory properties of murine iPSCs compared to MSCs using modified mixed leukocyte reactions. Our comparisons revealed that iPSCs generated through both lentiviral and piggyBac reprogramming methods had similar immunogenic properties as MSCs, and more potent immunomodulatory effects than MSCs. This information is critical when considering the use of iPSCs in the place of MSCs for both regenerative medicine and transplant medicine. Further studies must be performed, however, in order to determine if iPSCs retain their immunogenic and immunomodulatory properties upon differentiation into specific cell or tissue types. With this knowledge, we then shifted the focus of our third study to the horse, which is a valuable model for the human immune response. The purposes of this study were to immunophenotype MSCs from horses of known MHC haplotype and to compare the immunogenicity of MSCs with differing immunophenotypes, particularly in regards to MHC class II expression, through modified mixed leukocyte reactions. Results of this study demonstrated for the first time the extreme heterogeneity that exists in MHC class II expression by equine MSCs and that MHC class II positive equine MSCs are capable of inciting an immune response in vitro. This knowledge is critical for the treatment of our equine patients as well as for studies using the horse as an animal model for human diseases. Future experiments to determine if we can modulate this MHC class II expression in culture will be of great interest prior to… Advisors/Committee Members: Fortier, Lisa Ann (chair), Flaminio, Maria Julia Bevilaqua Felippe (committee member), Schimenti, John C. (committee member), Gilbert, Robert Owen (committee member), Nagy, Andras Bartos (committee member), Roberson, Mark Stephen (committee member).

Subjects/Keywords: Induced pluripotent stem cells (iPSCs); Mesenhcymal stem cells (MSCs); Immunonology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schnabel, L. (2013). Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/34305

Chicago Manual of Style (16^th Edition):

Schnabel, Lauren. “Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells.” 2013. Doctoral Dissertation, Cornell University. Accessed January 20, 2021. http://hdl.handle.net/1813/34305.

MLA Handbook (7^th Edition):

Schnabel, Lauren. “Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells.” 2013. Web. 20 Jan 2021.

Vancouver:

Schnabel L. Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1813/34305.

Council of Science Editors:

Schnabel L. Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34305

Uppsala University

28. Bartish, Margarita. Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome.

Degree: Biology Education Centre, 2012, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353

► The derivation of pluripotent stem cells (now termed induced pluripotent stem cells, iPSC) from mature somatic cells was a finding of seminal importance to… (more)

Subjects/Keywords: induced pluripotent stem cells; stem cells; disease modeling; cellular reprogramming

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bartish, M. (2012). Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bartish, Margarita. “Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome.” 2012. Thesis, Uppsala University. Accessed January 20, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bartish, Margarita. “Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome.” 2012. Web. 20 Jan 2021.

Vancouver:

Bartish M. Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome. [Internet] [Thesis]. Uppsala University; 2012. [cited 2021 Jan 20]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bartish M. Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome. [Thesis]. Uppsala University; 2012. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

29. GUNEWARDENE, NILIKSHA. The potential of induced pluripotent stem cells for spiral ganglion neuron replacement.

Degree: 2014, University of Melbourne

URL: http://hdl.handle.net/11343/43098

► In mammals, overexposure to loud noise, ototoxic medication or even ageing can incur irreversible damage to the sensory hair cells and spiral ganglion neurons (SGNs)… (more)

▼ In mammals, overexposure to loud noise, ototoxic medication or even ageing can incur irreversible damage to the sensory hair cells and spiral ganglion neurons (SGNs) resulting in sensorineural hearing loss (SNHL). Currently, the cochlear implant is the only available treatment for SNHL, but its functionality is dependent on a healthy complement of SGNs. Therefore in cases of severe SNHL, where the numbers of SGNs are significantly depleted, the efficacy of this neural prosthesis may be compromised. Using stem cells to replace damaged SGNs is an emerging therapeutic strategy for deafness. Whilst previous studies have explored the potential for several stem cell types, particularly human embryonic stem cells (hESCs) to replace SGNs, it will eventually be important that transplanted cells are from an autologous source. This thesis therefore aims to explore the potential of human induced pluripotent stem cells (hiPSCs) for SGN replacement. These cells offer the option of transplanting SGNs generated from a patient’s own cells to potentially restore hearing function and/or improve the efficiency of the cochlear implant. To investigate the potential of hiPSCs, it is first necessary to assess their potential to differentiate into a SGN lineage. In the first study of the thesis, an established neural induction protocol was used to differentiate two hiPSC lines (iPS1 and iPS2) and one human embryonic stem cell line (hESC, H9) towards a neurosensory lineage in vitro. Immunocytochemistry and qRT-PCR were used to analyse the expression of key markers involved in SGN development, at defined time points of differentiation. The hiPSC- and hESC-derived neurosensory progenitors expressed the dorsal hindbrain and otic placodal markers (PAX7 and PAX2), pro-neurosensory marker (SOX2), ganglion neuronal markers (NEUROD1, BRN3A, ISLET1, ßIII-tubulin, Neurofilament kDa 160) and sensory SGN markers (GATA3 and VGLUT1) over the time course examined. The hiPSC-and hESC-derived neurosensory progenitors had the highest expression levels of the sensory neural markers at 35 days in vitro. Whilst all cell lines analysed produced neurosensory-like progenitors, variabilities in the levels of marker expression were observed between hiPSC lines and within samples of the same cell line, when compared to the hESC controls. Thereby, suggesting that hiPSCs have a more variable differentiation potential compared to the hESCs. The functionality of the hiPSC-derived neurons was next assessed using patch clamp electrophysiology and in vitro co-culture assays. It was found that the cells were capable of firing action potentials in response to depolarisation and exhibited a phasic profile of activity, thus indicating that the neurons were physiologically active. Following co-culture of cochlear explants or denervated explants with hiPSC- and hESC-derived neurons, their neural processes were observed to make direct contact and form extensive synaptic connections with inner and outer hair cells in vitro. However the hiPSC-derived neurons were…

Subjects/Keywords: stem cells; spiral ganglion; cochlea; induced pluripotent stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

GUNEWARDENE, N. (2014). The potential of induced pluripotent stem cells for spiral ganglion neuron replacement. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/43098

Chicago Manual of Style (16^th Edition):

GUNEWARDENE, NILIKSHA. “The potential of induced pluripotent stem cells for spiral ganglion neuron replacement.” 2014. Doctoral Dissertation, University of Melbourne. Accessed January 20, 2021. http://hdl.handle.net/11343/43098.

MLA Handbook (7^th Edition):

GUNEWARDENE, NILIKSHA. “The potential of induced pluripotent stem cells for spiral ganglion neuron replacement.” 2014. Web. 20 Jan 2021.

Vancouver:

GUNEWARDENE N. The potential of induced pluripotent stem cells for spiral ganglion neuron replacement. [Internet] [Doctoral dissertation]. University of Melbourne; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11343/43098.

Council of Science Editors:

GUNEWARDENE N. The potential of induced pluripotent stem cells for spiral ganglion neuron replacement. [Doctoral Dissertation]. University of Melbourne; 2014. Available from: http://hdl.handle.net/11343/43098

UCLA

30. Chan, Ngam Fung David. Modeling Early Human Development with Human Pluripotent Stem Cells.

Degree: Molec, Cell, & Dev Biology, 2012, UCLA

URL: http://www.escholarship.org/uc/item/6wm0k0pd

► Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) are pluripotent stem cells (hPSCs) that have the capacity to differentiate into a panoply… (more)

▼ Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) are pluripotent stem cells (hPSCs) that have the capacity to differentiate into a panoply of various cell types and to serve as a model for human development. Nevertheless, it is unknown whether in vitro development of hPSCs mirrors in vivo human development. We performed comprehensive transcriptome profiling to compare hPSC-derived progeny with tissue-derived counterparts. While hESC and hiPSC make nearly identical progeny for the neural, hepatic and mesenchymal lineages, these hPSC-derived progeny maintained, regardless of the cell lineage, the expression of genes normally associated with early development, including LIN28A, LIN28B and DPPA4. These data and expression patterns in early human fetal tissue suggested the hPSC-derivatives were reflective of very early human development. The LIN28/let-7 pathway has been known to regulate development. We hypothesize that high Lin28 and low level let-7 activities may be inhibiting the developmental maturity in hPSC-derivatives. In addition, such a LIN28/let-7 expression pattern is also thought to contribute to tumor aggressiveness. We have established a human cell-based system to screen for small molecules that could modulate LIN28/let-7 activity. This screening could potentially unveil powerful small molecules that could modulate developmental maturity and therapeutically combat cancer. Previous reports and our own transcriptional profiling indicate that hPSCs are suitable for modeling early human development. Epithelial to mesenchymal transitions (EMTs) are thought to be essential to generate diversity of tissues during early fetal development, but these events are impossible to study at the molecular level in vivo in humans. The first EMT event in human development occurs just prior to generation of the primitive streak. Because hPSCs are thought to most closely resemble cells found in epiblast stage embryos prior to formation of the primitive streak, we sought to model this first human EMT in vitro with hPSCs. We have demonstrated the EMT progression in hPSCs with embryoid bodies. We also identified PTK7 as a marker of this EMT population, which was used to purify these cells for identification of novel markers of human germ layer development.

Subjects/Keywords: Developmental biology; Molecular biology; development; differentiation; embryonic stem cells; induced pluripotent stem cells; pluripotent stem cells; stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chan, N. F. D. (2012). Modeling Early Human Development with Human Pluripotent Stem Cells. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/6wm0k0pd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chan, Ngam Fung David. “Modeling Early Human Development with Human Pluripotent Stem Cells.” 2012. Thesis, UCLA. Accessed January 20, 2021. http://www.escholarship.org/uc/item/6wm0k0pd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chan, Ngam Fung David. “Modeling Early Human Development with Human Pluripotent Stem Cells.” 2012. Web. 20 Jan 2021.

Vancouver:

Chan NFD. Modeling Early Human Development with Human Pluripotent Stem Cells. [Internet] [Thesis]. UCLA; 2012. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/6wm0k0pd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chan NFD. Modeling Early Human Development with Human Pluripotent Stem Cells. [Thesis]. UCLA; 2012. Available from: http://www.escholarship.org/uc/item/6wm0k0pd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations