subject:(High Throughput Screening)

Ruhr Universität Bochum

1. Schuppert, Anna Katharina. Combinatorial screening of fuel cell catalysts for the oxygen reduction reaction.

Degree: 2014, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41410

► Brennstoffzellen sind aussichtsreiche Kandidaten um chemisch gespeicherte Energie (z.B. Wasserstoff aus erneuerbaren Energien) wieder in nutzbare elektrische Energie umzuwandeln. Die limitierende Reaktion ist dabei die… (more)

Subjects/Keywords: Katalyse; Brennstoffzelle; Platin; Elektrochemie; High throughput screening

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APA (6^th Edition):

Schuppert, A. K. (2014). Combinatorial screening of fuel cell catalysts for the oxygen reduction reaction. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schuppert, Anna Katharina. “Combinatorial screening of fuel cell catalysts for the oxygen reduction reaction.” 2014. Thesis, Ruhr Universität Bochum. Accessed January 19, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schuppert, Anna Katharina. “Combinatorial screening of fuel cell catalysts for the oxygen reduction reaction.” 2014. Web. 19 Jan 2021.

Vancouver:

Schuppert AK. Combinatorial screening of fuel cell catalysts for the oxygen reduction reaction. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Jan 19]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schuppert AK. Combinatorial screening of fuel cell catalysts for the oxygen reduction reaction. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McMaster University

2. Falconer, Shannon Beth. Chemical-genetics identifies two mechanistically unique spiro-analogs: an inhibitor of bacterial iron homeostasis and a zinc chelator that re-sensitizes a metallo-beta-lactamase-producing pathogen to carbapenem antibiotics.

Degree: PhD, 2014, McMaster University

URL: http://hdl.handle.net/11375/16101

►

Concomitant with antibiotic use is the development of bacterial strains that are resistant to such compounds. Presently, the rate at which antibiotic-resistant pathogenic bacteria are… (more)

Subjects/Keywords: Antibiotics; chemical genetics; high-throughput screening

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APA (6^th Edition):

Falconer, S. B. (2014). Chemical-genetics identifies two mechanistically unique spiro-analogs: an inhibitor of bacterial iron homeostasis and a zinc chelator that re-sensitizes a metallo-beta-lactamase-producing pathogen to carbapenem antibiotics. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16101

Chicago Manual of Style (16^th Edition):

Falconer, Shannon Beth. “Chemical-genetics identifies two mechanistically unique spiro-analogs: an inhibitor of bacterial iron homeostasis and a zinc chelator that re-sensitizes a metallo-beta-lactamase-producing pathogen to carbapenem antibiotics.” 2014. Doctoral Dissertation, McMaster University. Accessed January 19, 2021. http://hdl.handle.net/11375/16101.

MLA Handbook (7^th Edition):

Falconer, Shannon Beth. “Chemical-genetics identifies two mechanistically unique spiro-analogs: an inhibitor of bacterial iron homeostasis and a zinc chelator that re-sensitizes a metallo-beta-lactamase-producing pathogen to carbapenem antibiotics.” 2014. Web. 19 Jan 2021.

Vancouver:

Falconer SB. Chemical-genetics identifies two mechanistically unique spiro-analogs: an inhibitor of bacterial iron homeostasis and a zinc chelator that re-sensitizes a metallo-beta-lactamase-producing pathogen to carbapenem antibiotics. [Internet] [Doctoral dissertation]. McMaster University; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11375/16101.

Council of Science Editors:

Falconer SB. Chemical-genetics identifies two mechanistically unique spiro-analogs: an inhibitor of bacterial iron homeostasis and a zinc chelator that re-sensitizes a metallo-beta-lactamase-producing pathogen to carbapenem antibiotics. [Doctoral Dissertation]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16101

University of California – San Diego

3. Zhang, Siqi. High-throughput whole-animal screening using freshwater planarian as an alternative model for developmental neurotoxicity.

Degree: Bioengineering, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/9k5263nj

► The field of toxicology is under growing pressure to meet the demand of hazard assessment for the increasingly vast number of environmental toxicants. Due to… (more)

▼ The field of toxicology is under growing pressure to meet the demand of hazard assessment for the increasingly vast number of environmental toxicants. Due to low throughput and high cost, the traditional toxicity testing strategy, using rodent and higher mammalian animal-based models, is unable to adequately meet these competing demands. Hence, there is increasing recognition of the value of transforming toxicity testing to reduce the usage of mammal models and to more efficiently and reliably predict human relevant toxicity. To this end, a battery approach, integrating diverse in vitro and alternative animal models, was initiated to complement and accelerate hazard assessment. These alternative models are amenable to inexpensive, rapid and robust screening. In this dissertation, I introduce the asexual freshwater planarian Dugesia japonica as a novel alternative animal model to study developmental neurotoxicity. I developed and expanded a fully automated planarian high-throughput (HTS) screening platform to accomplish rapid screening of multiple morphological and behavioral endpoints. This HTS platform was evaluated for robustness, strengths and weaknesses, using an 87-compound library with known and suspected compounds and a 15-compound flame retardant library. As a unique advantage, the similar size of adult and regenerating planarians allows for direct comparison of two worm types to discern development-specific toxicity from overt systemic toxicity. We show that planarian is a useful model to potentially link toxicity pathways to whole-animal adverse functional outcomes by providing a large repertoire of behavioral endpoints. Comparative analysis of the planarian model with other alternative models, including zebrafish, nematode and in vitro cell-based models, provides insight into how different models complement each other in the battery approach. Finally, I discuss our comparative screen of organophosphrous pesticides (OPs) using the expanded platform, to investigate the possible mechanisms of developmental toxicity in OPs. Altogether, by establishing a new alternative animal model this work adds value to the battery approach to accelerate toxicity screening and prioritize toxicants, and provide further insight into the potential mechanisms of OP neurotoxicity.

Subjects/Keywords: Bioengineering; developmental neurotoxicology; high-throughput screening; planarian

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APA (6^th Edition):

Zhang, S. (2018). High-throughput whole-animal screening using freshwater planarian as an alternative model for developmental neurotoxicity. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/9k5263nj

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Siqi. “High-throughput whole-animal screening using freshwater planarian as an alternative model for developmental neurotoxicity.” 2018. Thesis, University of California – San Diego. Accessed January 19, 2021. http://www.escholarship.org/uc/item/9k5263nj.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Siqi. “High-throughput whole-animal screening using freshwater planarian as an alternative model for developmental neurotoxicity.” 2018. Web. 19 Jan 2021.

Vancouver:

Zhang S. High-throughput whole-animal screening using freshwater planarian as an alternative model for developmental neurotoxicity. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/9k5263nj.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang S. High-throughput whole-animal screening using freshwater planarian as an alternative model for developmental neurotoxicity. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/9k5263nj

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Harvard University

4. Matano, Leigh M. Accelerating the Discovery of Antibacterial Compounds to Validate Drug Targets and Probe Cell Physiology of S. Aureus.

Degree: PhD, 2017, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061471

►

Methicillin-resistant Staphylococcus aureus (MRSA) is a Gram-positive pathogen identified by its resistance to beta-lactam antibiotics. In an era where drug-resistant infections are becoming harder and… (more)

Subjects/Keywords: Antibiotics; drug discovery; high-throughput screening

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APA (6^th Edition):

Matano, L. M. (2017). Accelerating the Discovery of Antibacterial Compounds to Validate Drug Targets and Probe Cell Physiology of S. Aureus. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061471

Chicago Manual of Style (16^th Edition):

Matano, Leigh M. “Accelerating the Discovery of Antibacterial Compounds to Validate Drug Targets and Probe Cell Physiology of S. Aureus.” 2017. Doctoral Dissertation, Harvard University. Accessed January 19, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061471.

MLA Handbook (7^th Edition):

Matano, Leigh M. “Accelerating the Discovery of Antibacterial Compounds to Validate Drug Targets and Probe Cell Physiology of S. Aureus.” 2017. Web. 19 Jan 2021.

Vancouver:

Matano LM. Accelerating the Discovery of Antibacterial Compounds to Validate Drug Targets and Probe Cell Physiology of S. Aureus. [Internet] [Doctoral dissertation]. Harvard University; 2017. [cited 2021 Jan 19]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061471.

Council of Science Editors:

Matano LM. Accelerating the Discovery of Antibacterial Compounds to Validate Drug Targets and Probe Cell Physiology of S. Aureus. [Doctoral Dissertation]. Harvard University; 2017. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42061471

University of Texas Southwestern Medical Center

5. Zhong, Rui. Tackling Computational Challenges in High-Throughput RNA Interference Screening.

Degree: 2014, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/3335

► Since the discovery of RNAi decades ago, it has been increasingly used in biomedical and biological research. The success of analyzing single genes using siRNAs… (more)

Subjects/Keywords: High-Throughput Screening Assays; RNA Interference; Software

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APA (6^th Edition):

Zhong, R. (2014). Tackling Computational Challenges in High-Throughput RNA Interference Screening. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/3335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhong, Rui. “Tackling Computational Challenges in High-Throughput RNA Interference Screening.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed January 19, 2021. http://hdl.handle.net/2152.5/3335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhong, Rui. “Tackling Computational Challenges in High-Throughput RNA Interference Screening.” 2014. Web. 19 Jan 2021.

Vancouver:

Zhong R. Tackling Computational Challenges in High-Throughput RNA Interference Screening. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152.5/3335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhong R. Tackling Computational Challenges in High-Throughput RNA Interference Screening. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/3335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

6. Mandrell, David. Automated manipulation of zebrafish embryos for high-throughput toxicology screening of nanomaterials.

Degree: MS, Industrial Engineering, 2010, Oregon State University

URL: http://hdl.handle.net/1957/16495

► The field of nanotechnology has been rapidly developing new materials. These materials have become incorporated into consumer products and the environment after only minimal assessment… (more)

Subjects/Keywords: Automation; High throughput screening (Drug development)

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APA (6^th Edition):

Mandrell, D. (2010). Automated manipulation of zebrafish embryos for high-throughput toxicology screening of nanomaterials. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/16495

Chicago Manual of Style (16^th Edition):

Mandrell, David. “Automated manipulation of zebrafish embryos for high-throughput toxicology screening of nanomaterials.” 2010. Masters Thesis, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/16495.

MLA Handbook (7^th Edition):

Mandrell, David. “Automated manipulation of zebrafish embryos for high-throughput toxicology screening of nanomaterials.” 2010. Web. 19 Jan 2021.

Vancouver:

Mandrell D. Automated manipulation of zebrafish embryos for high-throughput toxicology screening of nanomaterials. [Internet] [Masters thesis]. Oregon State University; 2010. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/16495.

Council of Science Editors:

Mandrell D. Automated manipulation of zebrafish embryos for high-throughput toxicology screening of nanomaterials. [Masters Thesis]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/16495

University of Ottawa

7. Gaetz, Matthew. High-throughput siRNA Screen Identifies MTX2 as a Novel Mediator of Mitochondrial Morphology .

Degree: 2014, University of Ottawa

URL: http://hdl.handle.net/10393/31581

► Mitochondria exist in a dynamic network whereby fusion and fission events are critical to the health of the mitochondria, the cell, and the organism. Dysfunctional… (more)

Subjects/Keywords: Mitochondria; MTX2; High-throughput screening; RNAi

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APA (6^th Edition):

Gaetz, M. (2014). High-throughput siRNA Screen Identifies MTX2 as a Novel Mediator of Mitochondrial Morphology . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/31581

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gaetz, Matthew. “High-throughput siRNA Screen Identifies MTX2 as a Novel Mediator of Mitochondrial Morphology .” 2014. Thesis, University of Ottawa. Accessed January 19, 2021. http://hdl.handle.net/10393/31581.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gaetz, Matthew. “High-throughput siRNA Screen Identifies MTX2 as a Novel Mediator of Mitochondrial Morphology .” 2014. Web. 19 Jan 2021.

Vancouver:

Gaetz M. High-throughput siRNA Screen Identifies MTX2 as a Novel Mediator of Mitochondrial Morphology . [Internet] [Thesis]. University of Ottawa; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10393/31581.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gaetz M. High-throughput siRNA Screen Identifies MTX2 as a Novel Mediator of Mitochondrial Morphology . [Thesis]. University of Ottawa; 2014. Available from: http://hdl.handle.net/10393/31581

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

8. Koszela, Joanna. Novel screening methods for inhibitors of the human ubiquitin-conjugating enzymes.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/17893

► The ubiquitin-proteasome system (UPS) controls the stability, activity and localisation of most of the proteome and regulates virtually all cellular processes through modification of proteins… (more)

▼ The ubiquitin-proteasome system (UPS) controls the stability, activity and localisation of most of the proteome and regulates virtually all cellular processes through modification of proteins with ubiquitin. Ubiquitin conjugation is mediated by a conserved enzymatic cascade composed of E1, E2 and E3 enzymes, which cooperate to activate and transfer ubiquitin to substrate proteins. Dysfunction of the UPS is implicated in many disease states, including cancer, neurodegeneration, immune and cardiovascular disorders. Despite the central role of the UPS in cellular regulation, our understanding of the function, interactions and specificity of proteins that comprise the UPS is still limited. One approach to dissect and to study the UPS is to identify molecular probes, which can be used to specifically interrogate catalytic mechanisms and can be potentially considered as entry points for drug discovery. This work focuses on developing novel high-throughput screening methods for inhibitors of the ubiquitin-conjugating enzymes (E2s), using a unicellular organism Saccharomyces cerevisiae and in vitro technologies. S. cerevisiae is a model organism, commonly used in research as a valuable tool for genetic investigations and other high-throughput studies. In this work, we evaluated the toxicity of exogenously expressed human E2s on yeast cells and discovered that one of the E2s, Ube2U, significantly inhibited yeast growth. This inhibition was dependent on the Ube2U ubiquitin-conjugation activity, as demonstrated with a catalytically inactive Ube2U C89A control, which did not affect yeast growth. The growth defect induced by Ube2U allowed us to develop a screening setup for inhibitors of Ube2U, where the enzyme activity was coupled to cell growth readout. Potential Ube2U inhibitors would be identified as rescuers of the slow growing Ube2U-expressing yeast phenotype. Although screening methods in yeast are relatively straightforward to set up and run, the advantages of this system, namely simplicity of the detection signal and high-throughput, are limited by the fact that yeast is not a recognised large scale screening system in pharmaceutical industry, and that it is difficult to identify the target in a complex pathway such as the UPS. In vitro technologies are needed to provide the necessary structure-activity relationship for chemical optimisation. Therefore, we developed a novel, fluorescence-based, miniaturised assay technology, suitable for biochemical investigations and screening for inhibitors of a wide range of specific ubiquitination reactions within the UPS.

Subjects/Keywords: 572; ubiquitination; E2 enzymes; high-throughput screening

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Koszela, J. (2014). Novel screening methods for inhibitors of the human ubiquitin-conjugating enzymes. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/17893

Chicago Manual of Style (16^th Edition):

Koszela, Joanna. “Novel screening methods for inhibitors of the human ubiquitin-conjugating enzymes.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed January 19, 2021. http://hdl.handle.net/1842/17893.

MLA Handbook (7^th Edition):

Koszela, Joanna. “Novel screening methods for inhibitors of the human ubiquitin-conjugating enzymes.” 2014. Web. 19 Jan 2021.

Vancouver:

Koszela J. Novel screening methods for inhibitors of the human ubiquitin-conjugating enzymes. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1842/17893.

Council of Science Editors:

Koszela J. Novel screening methods for inhibitors of the human ubiquitin-conjugating enzymes. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/17893

University of Cambridge

9. Chong, Zheng Shan. Extracellular interaction screening using CRISPR activation.

Degree: PhD, 2019, University of Cambridge

URL: https://doi.org/10.17863/CAM.39124 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774750

► Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing… (more)

Subjects/Keywords: CRISPR; protein-protein interactions; high-throughput screening

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chong, Z. S. (2019). Extracellular interaction screening using CRISPR activation. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.39124 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774750

Chicago Manual of Style (16^th Edition):

Chong, Zheng Shan. “Extracellular interaction screening using CRISPR activation.” 2019. Doctoral Dissertation, University of Cambridge. Accessed January 19, 2021. https://doi.org/10.17863/CAM.39124 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774750.

MLA Handbook (7^th Edition):

Chong, Zheng Shan. “Extracellular interaction screening using CRISPR activation.” 2019. Web. 19 Jan 2021.

Vancouver:

Chong ZS. Extracellular interaction screening using CRISPR activation. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Jan 19]. Available from: https://doi.org/10.17863/CAM.39124 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774750.

Council of Science Editors:

Chong ZS. Extracellular interaction screening using CRISPR activation. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://doi.org/10.17863/CAM.39124 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774750

University of Texas – Austin

10. Ghorashian, Navid. Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans.

Degree: PhD, Biomedical Engineering, 2013, University of Texas – Austin

URL: http://hdl.handle.net/2152/46483

► With its well-characterized genome, simple anatomy, and vast array of uses in molecular biology, the roundworm, Caenorhabditis elegans (C. elegans) is a well-established model organism… (more)

▼ With its well-characterized genome, simple anatomy, and vast array of uses in molecular biology, the roundworm, Caenorhabditis elegans (C. elegans) is a well-established model organism in neurobiology. Concurrently, neurodegenerative diseases are some the most devastating and least understood ailments in modern medicine, making high-throughput approaches to understand their fundamental mechanisms imperative to developing new therapies. The worm's physical length-scales and simple genetics make it an ideal in vivo tool for high-throughput screening platforms. Concurrently, microfluidic technology has been used to make devices that manipulate these animals in a multitude of fashions to study various biological phenomena. With these considerations in mind, we have developed microfluidic platforms to facilitate optical interrogation of neurodegenerative and neuroregenerative phenomena in C. elegans for large-scale screens. First we developed a multiwell format device with 16 on-chip reservoirs to house and quickly deliver distinct worm populations to any liquid-format imaging platform. The system achieved unprecedented delivery speeds, avoided any population cross-contamination, and maintained animal viability. We then expanded this platform into a 64-well device that acted as a modular plug and play system for simple manipulation by conventional high-throughput liquid handling systems. The chip could be manipulated in the same fashion as a multiwell plate and interfaced with a novel pneumatic gasket system to achieve delivery speeds that were two-fold faster than those attained on the 16-well device. In addition, we worked to develop potential optical interrogation platform that could be fed populations of worms by the aforementioned delivery systems. This microfluidic chip consisted of an array of parallel traps to house individual worms over long durations for time-lapse studies of nerve regeneration after cuts to single axons mediated by a femtosecond pulse laser. Specifically, the platform was designed for regeneration studies in the C. elegans PQR neuron. Advisors/Committee Members: Ben-Yakar, Adela (advisor), Pierce-Shimomura, Jonathan T (committee member), Zhang, Xiaojing (committee member), Sokolov, Konstantin V (committee member), Dunn, Andrew K (committee member).

Subjects/Keywords: Neurobiology; Microfabrication; Microfluidics; Lasers; High-throughput screening

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APA (6^th Edition):

Ghorashian, N. (2013). Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/46483

Chicago Manual of Style (16^th Edition):

Ghorashian, Navid. “Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans.” 2013. Doctoral Dissertation, University of Texas – Austin. Accessed January 19, 2021. http://hdl.handle.net/2152/46483.

MLA Handbook (7^th Edition):

Ghorashian, Navid. “Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans.” 2013. Web. 19 Jan 2021.

Vancouver:

Ghorashian N. Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152/46483.

Council of Science Editors:

Ghorashian N. Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans. [Doctoral Dissertation]. University of Texas – Austin; 2013. Available from: http://hdl.handle.net/2152/46483

University of California – San Francisco

11. Ferreira, Rafaela. Combining virtual and high-throughput screening in the discovery of new cruzain inhibitors.

Degree: Chemistry and Chemical Biology, 2010, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/2509g569

► Cruzain's importance as a therapeutic target for Chagas Disease has been well established, and there is a continuous effort to find inhibitors for this cysteine… (more)

▼ Cruzain's importance as a therapeutic target for Chagas Disease has been well established, and there is a continuous effort to find inhibitors for this cysteine protease. Here I describe the use of both virtual and high throughput screening, followed by medicinal chemistry optimization, to identify and diversify inhibitors of cruzain. In the first study, reported in Chapter 1, virtual screening of the ZINC lead-like database led to the identification of two novel scaffolds of non-covalent cruzain inhibitors. Medicinal chemistry optimization of the most potent hit (Ki = 64 µM) resulted in a SAR series that included nanomolar inhbitors. However, more detailed studies on the most potent compounds revealed that they were aggregators. This study demonstrates for the first time an SAR due to aggregation. It is therefore a warning on the danger of considering SAR as definitive evidence for a specific mechanism of inhibition. Chapters 2-4 describe a collaboration with the NIH Chemical Genomics Center. We prospectively docked their 197,861 compound library, while they experimentally tested it versus cruzain by HTS. By running this side to side comparison, we were able to determine the main sources of false positives in HTS, to optimize a series of covalent reversible inhibitors found by HTS, and to evaluate in detail the false positives and false negatives from docking. Furthermore, five classes of non-covalent inhibitors were found, three of which were predicted by top scores in the dock ranked library. This study therefore indicates the potential for using virtual screening results to prioritize HTS hits. The work described here led to the identification of seven new classes of non covalent and one class of potent covalent reversible cruzain inhibitors. In addition, two high resolution crystal structures of this enzyme in complex with the new inhibitors were solved. Finally, this work highlights important lessons on the danger of simply interpreting SAR data, the false positives than can arise from HTS and docking, and the false negatives derived from docking.

Subjects/Keywords: Chemistry, Pharmaceutical; cruzain; high-throughput screening; virtual screening

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APA (6^th Edition):

Ferreira, R. (2010). Combining virtual and high-throughput screening in the discovery of new cruzain inhibitors. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/2509g569

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ferreira, Rafaela. “Combining virtual and high-throughput screening in the discovery of new cruzain inhibitors.” 2010. Thesis, University of California – San Francisco. Accessed January 19, 2021. http://www.escholarship.org/uc/item/2509g569.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ferreira, Rafaela. “Combining virtual and high-throughput screening in the discovery of new cruzain inhibitors.” 2010. Web. 19 Jan 2021.

Vancouver:

Ferreira R. Combining virtual and high-throughput screening in the discovery of new cruzain inhibitors. [Internet] [Thesis]. University of California – San Francisco; 2010. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/2509g569.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ferreira R. Combining virtual and high-throughput screening in the discovery of new cruzain inhibitors. [Thesis]. University of California – San Francisco; 2010. Available from: http://www.escholarship.org/uc/item/2509g569

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Abarca, Carla. High Throughput Screening of Nanoparticle Flotation Collectors.

Degree: PhD, 2017, McMaster University

URL: http://hdl.handle.net/11375/21064

►

Carla Abarca Ph.D. Thesis

The selective separation of valuable minerals by froth flotation is a critical unit operation in mineral processing. Froth flotation is based… (more)

▼

Carla Abarca Ph.D. Thesis

The selective separation of valuable minerals by froth flotation is a critical unit operation in mineral processing. Froth flotation is based on the ability of chemical reagents, called collectors, to selectively lower the surface energy of valuable mineral particles, facilitating attachment of the modified mineral particles to air bubbles in the flotation cell. The mineral laden bubbles rise to the surface forming a froth phase that can be isolated. Novel cationic polystyrene nanoparticle collectors have been developed recently to be used as effective flotation collectors, aiming to recover challenging nickel sulfide ores that respond poorly to conventional molecular flotation collectors. However, optimizing nanoparticle flotation collectors is a challenge. An effective nanoparticle collector candidate should meet three requirements: (1) it should be colloidally stable in the flotation media; (2) it should be hydrophobic enough to change the mineral surface and induce an air bubble-mineral particle attachment; and (3) specifically and strongly bind to metal-rich minerals. Producing nanoparticles that are simultaneously colloidally stable and sufficiently hydrophobic presents a problematic task. Thus, a delicate balance of nanoparticle properties is required for commercially viable nanoparticle collectors. This thesis presents a promising approach for discovering and characterizing novel nanoparticle collectors by using high throughput screening techniques. Developed was a workflow for fast fabrication and testing of nanoparticle candidates, including: (1) parallel production of large nanoparticle libraries covering a range of surface chemistries, (2) a high throughput colloidal stability assay to determine whether a nanoparticle type is stable in flotation conditions; (3) an automated contact angle assay to reject nanoparticles that are not hydrophobic enough to induce efficient bubble-particle attachment, and; (4) a laboratory flotation test in sodium carbonate (pH~10) with the best nanoparticle candidates. The automated colloidal stability assay was based on the optical characterization of diluted nanoparticle dispersions in multiwell plates, yielding critical coagulation concentrations (CCCs) of sodium carbonate. To pass this screening test, the CCC of candidate nanoparticles must be greater than the effective carbonate concentration in commercial flotation cells. Since the nanoparticle size affects the intrinsic light scattering properties of the nanoparticles, two routes were developed. The colloid stability assay was suitable for nanoparticles ranging between 50 nm and 500 nm, since nanoparticle size. The automated contact angle assay used a miniature 16-well plate format where flat glass slides were exposed to 200 μL nanoparticle dispersions. The cationic nanoparticles formed a saturated adsorbed monolayer on the glass, and after rinsing and drying, the water contact angle was automatically measured. Effective nanoparticle candidates had contact angles greater than 50…

Advisors/Committee Members: Pelton, Robert H., Chemical Engineering.

Subjects/Keywords: Nanoparticle Flotation Collectors; High Throughput Screening; Nanoparticles; Flotation; High Throughput; Click Chemistry

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APA (6^th Edition):

Abarca, C. (2017). High Throughput Screening of Nanoparticle Flotation Collectors. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/21064

Chicago Manual of Style (16^th Edition):

Abarca, Carla. “High Throughput Screening of Nanoparticle Flotation Collectors.” 2017. Doctoral Dissertation, McMaster University. Accessed January 19, 2021. http://hdl.handle.net/11375/21064.

MLA Handbook (7^th Edition):

Abarca, Carla. “High Throughput Screening of Nanoparticle Flotation Collectors.” 2017. Web. 19 Jan 2021.

Vancouver:

Abarca C. High Throughput Screening of Nanoparticle Flotation Collectors. [Internet] [Doctoral dissertation]. McMaster University; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11375/21064.

Council of Science Editors:

Abarca C. High Throughput Screening of Nanoparticle Flotation Collectors. [Doctoral Dissertation]. McMaster University; 2017. Available from: http://hdl.handle.net/11375/21064

Harvard University

13. Pardo, Carlos. Novel technologies for high-throughput and high-content studies on zebrafish larvae.

Degree: PhD, Engineering and Applied Sciences, 2013, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156677

►

The zebrafish larva is an ideal candidate for in vivo high-throughput screening: it is a small vertebrate, it is optically transparent, possesses complex organs, and… (more)

Subjects/Keywords: Engineering; Biomedical engineering; high-content; high-throughput; phenotyping; screening; tomography; zebrafish

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APA (6^th Edition):

Pardo, C. (2013). Novel technologies for high-throughput and high-content studies on zebrafish larvae. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156677

Chicago Manual of Style (16^th Edition):

Pardo, Carlos. “Novel technologies for high-throughput and high-content studies on zebrafish larvae.” 2013. Doctoral Dissertation, Harvard University. Accessed January 19, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156677.

MLA Handbook (7^th Edition):

Pardo, Carlos. “Novel technologies for high-throughput and high-content studies on zebrafish larvae.” 2013. Web. 19 Jan 2021.

Vancouver:

Pardo C. Novel technologies for high-throughput and high-content studies on zebrafish larvae. [Internet] [Doctoral dissertation]. Harvard University; 2013. [cited 2021 Jan 19]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156677.

Council of Science Editors:

Pardo C. Novel technologies for high-throughput and high-content studies on zebrafish larvae. [Doctoral Dissertation]. Harvard University; 2013. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156677

14. Helander, Jonathan Dean. Synthetic Agonists of Abscisic Acid Receptors and Their Metabolomic Effects on Plants.

Degree: Plant Biology, 2017, University of California – Riverside

URL: http://www.escholarship.org/uc/item/39k859cd

► Land plants respond to multiple abiotic stresses, including drought, through the signaling cascade induced by the phytohormone abscisic acid (ABA). Endogenous production, or externally applied… (more)

▼ Land plants respond to multiple abiotic stresses, including drought, through the signaling cascade induced by the phytohormone abscisic acid (ABA). Endogenous production, or externally applied ABA has a major function of eliciting guard cell closure, ultimately lowering transpiration and increasing drought tolerance in plants. Accordingly, the mechanisms of action in which ABA facilitates this response have been popular targets for agricultural research and applications. However, ABA has a multitude of responses aside from stomatal closure that are important for plant's survival to abiotic stress. In response to limited water availability, the phytohormone is known to maintain primary root growth while decreasing shoot growth, increase osmolyte accumulation, inhibit seed germination, and is involved in substantial crosstalk with other phytohormones. These responses are dependent on a core ABA signaling pathway comprised of three components: the ABA receptors known as the PYRABACTIN RESISTANT/PYRABACTIN RESISTANT-LIKE/REGULATORY COMPONENT OF ABA RECEPTORs (PYR/PYL/RCARs), the clade A protein phosphatase 2Cs (PP2Cs), and the sucrose nonfermenting related subfamily 2 (SnRK2s).The ABA receptors are the first interactors with ABA within the pathway, and cluster into three clades (I, II, III) based on sequence identity. Additionally, these clades can be further grouped based on oligomeric preference; clades I and II are preferentially monomeric, while clade III is preferentially dimeric. While some research has been done on non-redundant functions of the PYL proteins, many of the ABA responses remain uncharacterized with respect to the differential contributions of the different receptors. Additionally, most of the published ABA-receptor agonists are either direct ABA analogs displaying pan-agonist activity, or are primarily active only on the dimeric subgroup of the receptors. It would thus be potentially beneficial to develop agonists that show preferential activation of the monomeric receptors, allowing for temporal activation and subsequent analysis of their biological relevance.In order to identify compounds with novel selectivities, preferably on the monomeric receptors, I used high-throughput virtual screening to evaluate compounds unbiased to previous, active scaffolds. This resulted in a series of chemically similar hits which showed potent activity on the monomeric receptors, and translated to some in vivo responses. This potent, monomeric-specific scaffold was optimized using structure-aided design, improving the in vivo responses. Using this probe molecule I provide data that suggest that monomeric and dimeric ABA receptors may differentially control metabolomic and transcriptional responses, but are adequate in seed germination inhibition and primary root elongation.

Subjects/Keywords: Molecular biology; Amino acids; Glucosinolates; High throughput screening; HTS; Metabolomics; Virtual screening

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APA (6^th Edition):

Helander, J. D. (2017). Synthetic Agonists of Abscisic Acid Receptors and Their Metabolomic Effects on Plants. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/39k859cd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Helander, Jonathan Dean. “Synthetic Agonists of Abscisic Acid Receptors and Their Metabolomic Effects on Plants.” 2017. Thesis, University of California – Riverside. Accessed January 19, 2021. http://www.escholarship.org/uc/item/39k859cd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Helander, Jonathan Dean. “Synthetic Agonists of Abscisic Acid Receptors and Their Metabolomic Effects on Plants.” 2017. Web. 19 Jan 2021.

Vancouver:

Helander JD. Synthetic Agonists of Abscisic Acid Receptors and Their Metabolomic Effects on Plants. [Internet] [Thesis]. University of California – Riverside; 2017. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/39k859cd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Helander JD. Synthetic Agonists of Abscisic Acid Receptors and Their Metabolomic Effects on Plants. [Thesis]. University of California – Riverside; 2017. Available from: http://www.escholarship.org/uc/item/39k859cd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

15. Chebotarev, Oleg. High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/69980

►

The process of finding a new drug against a chosen target for a vascular disease usually involves high-throughput screening in static multi-well plates. However, due… (more)

Subjects/Keywords: Microfluidics; High throughput screening; Drug Screening; Monocyte adhesion; TNF-alpha; 0548; 0541

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APA (6^th Edition):

Chebotarev, O. (2013). High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/69980

Chicago Manual of Style (16^th Edition):

Chebotarev, Oleg. “High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments.” 2013. Masters Thesis, University of Toronto. Accessed January 19, 2021. http://hdl.handle.net/1807/69980.

MLA Handbook (7^th Edition):

Chebotarev, Oleg. “High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments.” 2013. Web. 19 Jan 2021.

Vancouver:

Chebotarev O. High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1807/69980.

Council of Science Editors:

Chebotarev O. High Throughput Microfluidic Platform Capable of Mimicking Key Vascular Microenvironments. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/69980

16. Firsova, Daria. Combining biological and chemical approaches in marine biodiscovery .

Degree: 2017, National University of Ireland – Galway

URL: http://hdl.handle.net/10379/6689

► Marine organisms are prolific producers of novel and structurally interesting metabolites. The identification of marine natural products contributes to many scientific areas including pharmacology, chemical… (more)

Subjects/Keywords: Marine natural products; High throughput screening; Colorectal cancer; Chemical screening; Sponges; Chemistry

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APA (6^th Edition):

Firsova, D. (2017). Combining biological and chemical approaches in marine biodiscovery . (Thesis). National University of Ireland – Galway. Retrieved from http://hdl.handle.net/10379/6689

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Firsova, Daria. “Combining biological and chemical approaches in marine biodiscovery .” 2017. Thesis, National University of Ireland – Galway. Accessed January 19, 2021. http://hdl.handle.net/10379/6689.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Firsova, Daria. “Combining biological and chemical approaches in marine biodiscovery .” 2017. Web. 19 Jan 2021.

Vancouver:

Firsova D. Combining biological and chemical approaches in marine biodiscovery . [Internet] [Thesis]. National University of Ireland – Galway; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10379/6689.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Firsova D. Combining biological and chemical approaches in marine biodiscovery . [Thesis]. National University of Ireland – Galway; 2017. Available from: http://hdl.handle.net/10379/6689

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

17. Kurbanov, Elbek. Synthetic, Biochemical, X-ray Crystallographic, Computational and High-Throughput Screening Approaches Toward Anthrax Toxin Lethal Factor Inhibition.

Degree: PhD, Medicinal Chemistry, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/192665

► The lethal factor (LF) enzyme secreted by Bacillus anthracis is chiefly responsible for anthrax-related cytotoxicity. In this dissertation, I present the computational design, synthesis, biochemical… (more)

▼ The lethal factor (LF) enzyme secreted by Bacillus anthracis is chiefly responsible for anthrax-related cytotoxicity. In this dissertation, I present the computational design, synthesis, biochemical testing, structural biology, and virtual and high-throughput screening approaches to identify binding requirements for LF inhibition. To this end, we designed ~50 novel compounds to probe design principles and structural requirements for LF. Specifically, in Chapters 2 and 3, computational, synthetic, biochemical and structural biology methods to explore the underinvestigated LF S2′ binding subsite are described. We discovered that LF domain 3 is very flexible and results in a relatively unconstrained S2′ binding site region. Additionally, we found that the S1′ subsite can undergo a novel conformational change resulting in a previously unreported tunnel region, which we term S1′*, that we expect can further be explored to design potent and selective LF inhibitors. Using this novel LF configuration, we virtually screened ~11 million drug-like compounds for activity against LF and have identified a novel compound that inhibits LF with an IC50 of 126 μM. In the course of this work, we found that reliable representation of zinc and other transition metal centers in macromolecules is nontrivial, due to the complexity of the coordination environment and charge distribution at the catalytic center. In Chapter 7, I will present work on applying and optimizing quantum mechanical methods developed by the Truhlar group to accurately calculate bond dissociation energies at low computational cost for various representative Zn2+ and Cd2+ model systems. By analyzing errors, we developed a prescription for an optimal system fragmentation strategy for our models. With this scheme, we find that the EE-3B-CE method is able to reproduce 53 conventionally calculated bond energies with an average absolute error of only 0.59 kcal/mol. Therefore, one could use the EE 3B CE approximation to obtain accurate results for large systems and/or identify better parameters for Zn centers for use in virtual screening. Finally, we present the results of a large-scale in vitro HTS campaign of ~250,000 small-molecules against LF. After extensive validation, involving secondary assays and hit synthesis we were able to prioritize a key lead for further prosecution.

Subjects/Keywords: anthrax; computational chemistry; high-throughput screening; lethal factor; structure-based inhibitor design; virtual screening

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APA (6^th Edition):

Kurbanov, E. (2015). Synthetic, Biochemical, X-ray Crystallographic, Computational and High-Throughput Screening Approaches Toward Anthrax Toxin Lethal Factor Inhibition. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/192665

Chicago Manual of Style (16^th Edition):

Kurbanov, Elbek. “Synthetic, Biochemical, X-ray Crystallographic, Computational and High-Throughput Screening Approaches Toward Anthrax Toxin Lethal Factor Inhibition.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 19, 2021. http://hdl.handle.net/11299/192665.

MLA Handbook (7^th Edition):

Kurbanov, Elbek. “Synthetic, Biochemical, X-ray Crystallographic, Computational and High-Throughput Screening Approaches Toward Anthrax Toxin Lethal Factor Inhibition.” 2015. Web. 19 Jan 2021.

Vancouver:

Kurbanov E. Synthetic, Biochemical, X-ray Crystallographic, Computational and High-Throughput Screening Approaches Toward Anthrax Toxin Lethal Factor Inhibition. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11299/192665.

Council of Science Editors:

Kurbanov E. Synthetic, Biochemical, X-ray Crystallographic, Computational and High-Throughput Screening Approaches Toward Anthrax Toxin Lethal Factor Inhibition. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/192665

Ruhr Universität Bochum

18. Cao, Jialin. High-throughput characterization of hydrogen storage in thin films using Si-cantilever arrays.

Degree: 2008, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-21835

► In der vorliegenden Arbeit wurde eine neue Hochdurchsatz-Charakterisierungsmethode entwickelt, um Wasserstoffspeicherung in dünnen Schichten mittels mikrostrukturierter Si-Biegebalken in Matrixanordnung zu untersuchen. Die Wasserstoffspeicher-Eigenschaften der dünnen… (more)

Subjects/Keywords: Wasserstoffspeicherung; Dünne Schicht; Kombinatorik; High throughput screening; Kantilever

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APA (6^th Edition):

Cao, J. (2008). High-throughput characterization of hydrogen storage in thin films using Si-cantilever arrays. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-21835

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cao, Jialin. “High-throughput characterization of hydrogen storage in thin films using Si-cantilever arrays.” 2008. Thesis, Ruhr Universität Bochum. Accessed January 19, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-21835.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cao, Jialin. “High-throughput characterization of hydrogen storage in thin films using Si-cantilever arrays.” 2008. Web. 19 Jan 2021.

Vancouver:

Cao J. High-throughput characterization of hydrogen storage in thin films using Si-cantilever arrays. [Internet] [Thesis]. Ruhr Universität Bochum; 2008. [cited 2021 Jan 19]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-21835.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cao J. High-throughput characterization of hydrogen storage in thin films using Si-cantilever arrays. [Thesis]. Ruhr Universität Bochum; 2008. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-21835

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

19. Vukojević, Sascha. Copper colloids and nanoparticular Cu/ZrO2-based catalysts for methanol synthesis.

Degree: 2009, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-26844

► In der vorliegenden Dissertation wurden nanopartikulare Cu/ZrO2-basierende Feststoffkatalysatoren auf drei verschiedene Arten synthetisiert: Exotemplating mit Aktivkohle, Mikroemulsionstechnik sowie Imprägnierung von Silica-Monolithen, und mit Hilfe von… (more)

Subjects/Keywords: Methanolherstellung; Heterogene Katalyse; EXAFS; Kupfer / Kolloid; High Throughput screening

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APA (6^th Edition):

Vukojević, S. (2009). Copper colloids and nanoparticular Cu/ZrO2-based catalysts for methanol synthesis. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-26844

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vukojević, Sascha. “Copper colloids and nanoparticular Cu/ZrO2-based catalysts for methanol synthesis.” 2009. Thesis, Ruhr Universität Bochum. Accessed January 19, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-26844.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vukojević, Sascha. “Copper colloids and nanoparticular Cu/ZrO2-based catalysts for methanol synthesis.” 2009. Web. 19 Jan 2021.

Vancouver:

Vukojević S. Copper colloids and nanoparticular Cu/ZrO2-based catalysts for methanol synthesis. [Internet] [Thesis]. Ruhr Universität Bochum; 2009. [cited 2021 Jan 19]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-26844.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vukojević S. Copper colloids and nanoparticular Cu/ZrO2-based catalysts for methanol synthesis. [Thesis]. Ruhr Universität Bochum; 2009. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-26844

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Santa Cruz

20. Woehrmann, Marcos H. Predicting the Mode of Action of Bioactive Compounds via High Throughput Screening and Computational Algorithms.

Degree: Biomolecular Engineering and Bioinformatics, 2015, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/9976p4ch

► To develop more effective therapies to treat human diseases, a better method of finding the biological targets and modes of action of new compounds is… (more)

▼ To develop more effective therapies to treat human diseases, a better method of finding the biological targets and modes of action of new compounds is needed. Target predictions have traditionally been made by comparing a new compound's molecular structure to that of known compounds. In many cases this method does not accurately predict a chemical's function since "small chemical changes in an active molecule can render it either nearly or completely inactive or increase its activity dramatically" (Eckert and Bajorath, 2007). Further, prediction by structural comparison has limited application; it can only be used on chemicals with established structures and only identifies new compounds that are structurally similar to known compounds.A majority of existing drugs have been discovered by identifying the active ingredient of traditional medicines. More recent techniques of drug discovery screen a library of compounds for effectiveness in treating a single disease. However, this method requires re-screening the library when searching for treatments for other diseases; a critical barrier to expediting and scaling drug discovery. Screening efficiency is particularly important since advances in robotic chemical synthesis and the search for natural products from the oceans are rapidly increasing the size of drug candidate libraries.In contrast to current approaches which screen compounds for treatments for single disease; my research focused on creating screening methods that deliver a library of chemical fingerprints which can be used to find potential drug candidates for a multiplicity of diseases.My work produced three screening methods that generate fingerprints useful for predicting a compound's mode of action: cytological profiling, D- Map, and BioSpace. All of these showed positive results towards solving the screening bottleneck. Finally, combining these approaches to integrate these various fingerprints could increase prediction accuracy of screening methods.

Subjects/Keywords: Bioinformatics; Bioinformatics; Cytological Profiling; Drug Target Prediction; High-throughput Screening; HTS

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APA (6^th Edition):

Woehrmann, M. H. (2015). Predicting the Mode of Action of Bioactive Compounds via High Throughput Screening and Computational Algorithms. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/9976p4ch

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Woehrmann, Marcos H. “Predicting the Mode of Action of Bioactive Compounds via High Throughput Screening and Computational Algorithms.” 2015. Thesis, University of California – Santa Cruz. Accessed January 19, 2021. http://www.escholarship.org/uc/item/9976p4ch.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Woehrmann, Marcos H. “Predicting the Mode of Action of Bioactive Compounds via High Throughput Screening and Computational Algorithms.” 2015. Web. 19 Jan 2021.

Vancouver:

Woehrmann MH. Predicting the Mode of Action of Bioactive Compounds via High Throughput Screening and Computational Algorithms. [Internet] [Thesis]. University of California – Santa Cruz; 2015. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/9976p4ch.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Woehrmann MH. Predicting the Mode of Action of Bioactive Compounds via High Throughput Screening and Computational Algorithms. [Thesis]. University of California – Santa Cruz; 2015. Available from: http://www.escholarship.org/uc/item/9976p4ch

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Pushkarsky, Ivan. High-throughput analysis of single-cell force biology.

Degree: Bioengineering, 2017, UCLA

URL: http://www.escholarship.org/uc/item/4tq4961w

► Mechanical forces generated by cells play important functional roles within many physiological systems such as regulating local vascular resistance, producing cardiac contractions, and propelling intestinal… (more)

▼ Mechanical forces generated by cells play important functional roles within many physiological systems such as regulating local vascular resistance, producing cardiac contractions, and propelling intestinal contents. As a result, a multitude of disorders can be caused directly by malfunctioning cellular force generation including chronic conditions such as asthma, hypertension, and bowel disease. Such disorders place a significant burden on patient health and there is a paucity of effective drugs, arguably due to a lack of adequate tools for evaluating cellular force in research or in early drug discovery. Existing approaches to measure cellular forces have been largely limited to biological research settings. Of these, the most widely used are traction force microscopy (TFM) and elastomeric micropost arrays (EMA). Although both approaches are useful for probing specific sub-cellular forces at low yields (usually 10s of cells per experiment), they were not intended to be used as high-throughput tools for rapidly conducting extensive pharmacological testing and have not been adopted by pharmaceutical companies for drug discovery. Specific barriers to adoption of such technologies include their relatively low throughput, complex experimental procedures which are difficult to automate, and minimal control over cell morphologies which necessitates intensive, non-trivial data analysis and normalization. Production of these systems and their physical characteristics also complicate or entirely prevent integration with standard SLAS well-plate formats. We developed a high-throughput phenotypic screening platform that met this need by measuring the relative strains induced by the contractile forces generated by up to 105 single-cells adhered to large arrays of elastomeric surface sensors. The method supports analysis of a variety of force-generating behaviors in both a standalone and the multiwell formats used for drug-discovery. This dissertation describes the fabrication of these novel substrates, provides several use-cases including studies of smooth muscle or cardiac muscle force biology at the single-cell level, and provides novel biological data regarding the phagocytic forces of individual human macrophages.We conclude by demonstrating the integration of the platform into standard 96- and 384-wellplates and verify its feasibility as a drug discovery tool by performing a pilot high-throughput screen to identifying potential bronchodilatory compounds for asthma management. These results indicate this platform could aid in the discovery of not only new therapeutic compounds, but also of novel molecular mechanisms associated with disease.

Subjects/Keywords: Biomedical engineering; actin; cell mechanics; cellular forces; high-throughput screening; phagocytosis

12 more images…

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APA (6^th Edition):

Pushkarsky, I. (2017). High-throughput analysis of single-cell force biology. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/4tq4961w

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pushkarsky, Ivan. “High-throughput analysis of single-cell force biology.” 2017. Thesis, UCLA. Accessed January 19, 2021. http://www.escholarship.org/uc/item/4tq4961w.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pushkarsky, Ivan. “High-throughput analysis of single-cell force biology.” 2017. Web. 19 Jan 2021.

Vancouver:

Pushkarsky I. High-throughput analysis of single-cell force biology. [Internet] [Thesis]. UCLA; 2017. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/4tq4961w.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pushkarsky I. High-throughput analysis of single-cell force biology. [Thesis]. UCLA; 2017. Available from: http://www.escholarship.org/uc/item/4tq4961w

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

22. Beck, Sungjun. Mechanism of Action of Methotrexate Against Zika Virus.

Degree: Biology, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/4ch0p661

► Zika Virus (ZIKV), which is associated with microcephaly and Guillain-Barré syndrome, brought a serious public health impact to most of Latin America countries in 2015… (more)

Subjects/Keywords: Biology; Dihydrofolate reductase; High throughput screening; Methotrexate; Zika virus

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APA (6^th Edition):

Beck, S. (2018). Mechanism of Action of Methotrexate Against Zika Virus. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/4ch0p661

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Beck, Sungjun. “Mechanism of Action of Methotrexate Against Zika Virus.” 2018. Thesis, University of California – San Diego. Accessed January 19, 2021. http://www.escholarship.org/uc/item/4ch0p661.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Beck, Sungjun. “Mechanism of Action of Methotrexate Against Zika Virus.” 2018. Web. 19 Jan 2021.

Vancouver:

Beck S. Mechanism of Action of Methotrexate Against Zika Virus. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/4ch0p661.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Beck S. Mechanism of Action of Methotrexate Against Zika Virus. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/4ch0p661

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

San Jose State University

23. Blankenship, Lauren Renee. Increasing Colistin Susceptibility in Antibiotic-Resistant Pseudomonas aeruginosa.

Degree: MS, Chemistry, 2016, San Jose State University

URL: https://doi.org/10.31979/etd.u9n7-x4uc ; https://scholarworks.sjsu.edu/etd_theses/4747

► Pseudomonas aeruginosa is a gram-negative bacterium that causes hospital-acquired infections. P. aeruginosa has a multitude of defense mechanisms that limit the effectiveness of antibiotics… (more)

Subjects/Keywords: antibiotic resistance; colistin; high throughput screening; organic synthesis; Pseudomonas aeruginosa

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APA (6^th Edition):

Blankenship, L. R. (2016). Increasing Colistin Susceptibility in Antibiotic-Resistant Pseudomonas aeruginosa. (Masters Thesis). San Jose State University. Retrieved from https://doi.org/10.31979/etd.u9n7-x4uc ; https://scholarworks.sjsu.edu/etd_theses/4747

Chicago Manual of Style (16^th Edition):

Blankenship, Lauren Renee. “Increasing Colistin Susceptibility in Antibiotic-Resistant Pseudomonas aeruginosa.” 2016. Masters Thesis, San Jose State University. Accessed January 19, 2021. https://doi.org/10.31979/etd.u9n7-x4uc ; https://scholarworks.sjsu.edu/etd_theses/4747.

MLA Handbook (7^th Edition):

Blankenship, Lauren Renee. “Increasing Colistin Susceptibility in Antibiotic-Resistant Pseudomonas aeruginosa.” 2016. Web. 19 Jan 2021.

Vancouver:

Blankenship LR. Increasing Colistin Susceptibility in Antibiotic-Resistant Pseudomonas aeruginosa. [Internet] [Masters thesis]. San Jose State University; 2016. [cited 2021 Jan 19]. Available from: https://doi.org/10.31979/etd.u9n7-x4uc ; https://scholarworks.sjsu.edu/etd_theses/4747.

Council of Science Editors:

Blankenship LR. Increasing Colistin Susceptibility in Antibiotic-Resistant Pseudomonas aeruginosa. [Masters Thesis]. San Jose State University; 2016. Available from: https://doi.org/10.31979/etd.u9n7-x4uc ; https://scholarworks.sjsu.edu/etd_theses/4747

San Jose State University

24. Tiongco, Richard Paul Manarin. High-Throughput Screening of a Metagenomic Library Using a Novel E. Coli Chip for Discovery of Antibiotic Resistance Genes.

Degree: MS, Engineering, 2019, San Jose State University

URL: https://scholarworks.sjsu.edu/etd_theses/5084

► The growing prevalence of antibiotic resistance is one of the greatest challenges facing humankind today. The Centers for Disease Control and Prevention (CDC) have… (more)

Subjects/Keywords: High-Throughput Screening; lab on a chip; Metagenomics

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APA (6^th Edition):

Tiongco, R. P. M. (2019). High-Throughput Screening of a Metagenomic Library Using a Novel E. Coli Chip for Discovery of Antibiotic Resistance Genes. (Masters Thesis). San Jose State University. Retrieved from https://scholarworks.sjsu.edu/etd_theses/5084

Chicago Manual of Style (16^th Edition):

Tiongco, Richard Paul Manarin. “High-Throughput Screening of a Metagenomic Library Using a Novel E. Coli Chip for Discovery of Antibiotic Resistance Genes.” 2019. Masters Thesis, San Jose State University. Accessed January 19, 2021. https://scholarworks.sjsu.edu/etd_theses/5084.

MLA Handbook (7^th Edition):

Tiongco, Richard Paul Manarin. “High-Throughput Screening of a Metagenomic Library Using a Novel E. Coli Chip for Discovery of Antibiotic Resistance Genes.” 2019. Web. 19 Jan 2021.

Vancouver:

Tiongco RPM. High-Throughput Screening of a Metagenomic Library Using a Novel E. Coli Chip for Discovery of Antibiotic Resistance Genes. [Internet] [Masters thesis]. San Jose State University; 2019. [cited 2021 Jan 19]. Available from: https://scholarworks.sjsu.edu/etd_theses/5084.

Council of Science Editors:

Tiongco RPM. High-Throughput Screening of a Metagenomic Library Using a Novel E. Coli Chip for Discovery of Antibiotic Resistance Genes. [Masters Thesis]. San Jose State University; 2019. Available from: https://scholarworks.sjsu.edu/etd_theses/5084

Kyoto University / 京都大学

25. Nishihara, Kana. Identification of Genotoxic Compounds Using Isogenic DNA Repair Deficient DT40 Cell Lines on a Quantitative High Throughput Screening Platform : DNA損傷修復欠損DT40細胞を用いた定量的ハイスループットスクリーニングによる遺伝毒性物質の同定.

Degree: 博士(医学), 2016, Kyoto University / 京都大学

URL: http://hdl.handle.net/2433/215414 ; http://dx.doi.org/10.14989/doctor.k19588

This is a pre-copyedited, author-produced PDF of an article accepted for publication in Mutagenesis following peer review. The definitive publisher-authenticated version is available online at:http://mutage.oxfordjournals.org/content/early/2015/08/03/mutage.gev055.full.

新制・課程博士

甲第19588号

医博第4095号

Subjects/Keywords: DT40; High Throughput Screening; DNA Repair; Genotoxic; Tox21

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APA (6^th Edition):

Nishihara, K. (2016). Identification of Genotoxic Compounds Using Isogenic DNA Repair Deficient DT40 Cell Lines on a Quantitative High Throughput Screening Platform : DNA損傷修復欠損DT40細胞を用いた定量的ハイスループットスクリーニングによる遺伝毒性物質の同定. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/215414 ; http://dx.doi.org/10.14989/doctor.k19588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nishihara, Kana. “Identification of Genotoxic Compounds Using Isogenic DNA Repair Deficient DT40 Cell Lines on a Quantitative High Throughput Screening Platform : DNA損傷修復欠損DT40細胞を用いた定量的ハイスループットスクリーニングによる遺伝毒性物質の同定.” 2016. Thesis, Kyoto University / 京都大学. Accessed January 19, 2021. http://hdl.handle.net/2433/215414 ; http://dx.doi.org/10.14989/doctor.k19588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nishihara, Kana. “Identification of Genotoxic Compounds Using Isogenic DNA Repair Deficient DT40 Cell Lines on a Quantitative High Throughput Screening Platform : DNA損傷修復欠損DT40細胞を用いた定量的ハイスループットスクリーニングによる遺伝毒性物質の同定.” 2016. Web. 19 Jan 2021.

Vancouver:

Nishihara K. Identification of Genotoxic Compounds Using Isogenic DNA Repair Deficient DT40 Cell Lines on a Quantitative High Throughput Screening Platform : DNA損傷修復欠損DT40細胞を用いた定量的ハイスループットスクリーニングによる遺伝毒性物質の同定. [Internet] [Thesis]. Kyoto University / 京都大学; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2433/215414 ; http://dx.doi.org/10.14989/doctor.k19588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nishihara K. Identification of Genotoxic Compounds Using Isogenic DNA Repair Deficient DT40 Cell Lines on a Quantitative High Throughput Screening Platform : DNA損傷修復欠損DT40細胞を用いた定量的ハイスループットスクリーニングによる遺伝毒性物質の同定. [Thesis]. Kyoto University / 京都大学; 2016. Available from: http://hdl.handle.net/2433/215414 ; http://dx.doi.org/10.14989/doctor.k19588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

26. Tague, Michele. A Combinatorial Approach For Exploring Fuel Cell Electrocatalysts.

Degree: PhD, Chemistry and Chemical Biology, 2011, Cornell University

URL: http://hdl.handle.net/1813/30690

► Fuel cells represent an alternative energy technology with the potential for providing more efficient means for energy conversion. However, their widespread deployment has been hampered… (more)

Subjects/Keywords: Methanol oxidation; fuel cells; Platinum alloys; combinatorial screening; Fluorescence; high throughput

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APA (6^th Edition):

Tague, M. (2011). A Combinatorial Approach For Exploring Fuel Cell Electrocatalysts. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/30690

Chicago Manual of Style (16^th Edition):

Tague, Michele. “A Combinatorial Approach For Exploring Fuel Cell Electrocatalysts.” 2011. Doctoral Dissertation, Cornell University. Accessed January 19, 2021. http://hdl.handle.net/1813/30690.

MLA Handbook (7^th Edition):

Tague, Michele. “A Combinatorial Approach For Exploring Fuel Cell Electrocatalysts.” 2011. Web. 19 Jan 2021.

Vancouver:

Tague M. A Combinatorial Approach For Exploring Fuel Cell Electrocatalysts. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1813/30690.

Council of Science Editors:

Tague M. A Combinatorial Approach For Exploring Fuel Cell Electrocatalysts. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30690

University of Michigan

27. Guetschow, Erik D. Droplet Microfluidics Coupled to Microchip Electrophoresis for High-Throughput Enzyme Modulator Screening.

Degree: PhD, Chemistry, 2016, University of Michigan

URL: http://hdl.handle.net/2027.42/120766

► High-throughput screening (HTS) represents a powerful tool for drug discovery by allowing tens of thousands of assays to be completed within a single day. Typically,… (more)

▼ High-throughput screening (HTS) represents a powerful tool for drug discovery by allowing tens of thousands of assays to be completed within a single day. Typically, assays are performed in multiwell plates (MWPs) utilizing fluorogenic substrates for detection. These substrates increase development time and can introduce artifactual results. Therefore, alternate screening strategies based around natural substrates are necessary. We developed a screening platform that couples nanoliter volume samples to microchip electrophoresis for analysis using a novel polydimethylsiloxane (PDMS)-and-glass microfluidic device. The system was demonstrated by screening a small library against protein kinase A (PKA), which regulates metabolism within the cell. It was chosen for its well-characterized kinetic parameters and commercially available peptide substrates. Sample throughput of 0.16 Hz was achieved allowing at least 6 replicate MCE injections from each sample in a high quality assay (Z’-factor = 0.8). To demonstrate the ability to screen larger libraries, we developed a novel assay for sirtuin 5 (SIRT5) using a naturally derived peptide substrate. SIRT5 impacts metabolism and has reported oncogenic functions making inhibitor identification of clinical importance. Compared to the PKA assay previously developed, assay throughput was increased 3-fold and 1406 samples were analyzed within 46 minutes (0.5 Hz). Using a 250 ms separation, each assay sample could be analyzed 8 times by MCE generating over 11,000 electropherograms. Several previously unreported inhibitors of SIRT5 were identified and verified by dose-response analysis. Finally, work toward miniaturization of high-throughput assays was demonstrated by performing sample preparation and analysis completely within nanoliter volume droplets. A simple to use and easy to fabricate PDMS microfluidic device was developed to allow addition of assay reagents to nanoliter volume samples. Reagent use, relative to assays performed in 384 well plates, could be reduced 1,000-fold and sample-to-sample carryover was less than 5 percent under typical experimental conditions. Analysis of samples prepared in droplet format was demonstrated with droplets containing a fluorescent dye and addition of a fluorescent peptide. These samples could be analyzed by MCE at 0.33 samples per second but increased throughput should be possible by using higher flow rates. Advisors/Committee Members: Kennedy, Robert T. (committee member), Chronis, Nikolaos (committee member), Meyerhoff, Mark E (committee member), Ruotolo, Brandon Thomas (committee member).

Subjects/Keywords: Microfluidics; Electrophoresis; segmented flow; High-throughput screening; Chemistry; Science

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APA (6^th Edition):

Guetschow, E. D. (2016). Droplet Microfluidics Coupled to Microchip Electrophoresis for High-Throughput Enzyme Modulator Screening. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/120766

Chicago Manual of Style (16^th Edition):

Guetschow, Erik D. “Droplet Microfluidics Coupled to Microchip Electrophoresis for High-Throughput Enzyme Modulator Screening.” 2016. Doctoral Dissertation, University of Michigan. Accessed January 19, 2021. http://hdl.handle.net/2027.42/120766.

MLA Handbook (7^th Edition):

Guetschow, Erik D. “Droplet Microfluidics Coupled to Microchip Electrophoresis for High-Throughput Enzyme Modulator Screening.” 2016. Web. 19 Jan 2021.

Vancouver:

Guetschow ED. Droplet Microfluidics Coupled to Microchip Electrophoresis for High-Throughput Enzyme Modulator Screening. [Internet] [Doctoral dissertation]. University of Michigan; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2027.42/120766.

Council of Science Editors:

Guetschow ED. Droplet Microfluidics Coupled to Microchip Electrophoresis for High-Throughput Enzyme Modulator Screening. [Doctoral Dissertation]. University of Michigan; 2016. Available from: http://hdl.handle.net/2027.42/120766

Texas A&M University

28. Haynes, Abria R. Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries.

Degree: PhD, Plant Pathology & Microbiology, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/152602

► Industrial bioprocesses are constrained by the availability of microbes that are optimized for harsh bioprocess conditions. Over 500 soil and sediment samples collected from 77… (more)

▼ Industrial bioprocesses are constrained by the availability of microbes that are optimized for harsh bioprocess conditions. Over 500 soil and sediment samples collected from 77 saline and/or thermal sites in the continental United States, Hawaii, and Puerto Rico were used to inoculate fermentations in an effort to optimize the microbial communities for a biofuel process developed at Texas A&M (MixAlco™). A diverse bacterial isolate library (N= 1,866 isolates) was constructed by employing a variety of culturing techniques across thirty-four of the fermentation communities selected based on superior performance in the biofuel platform. Phylogenetic analysis of partial 16S rDNA sequences placed 1,200 of these isolates in the genus Bacillus. Other genera include, but are not limited to: Ureibacillus, Bacillus, Geobacillus, and Caldalkalibacillus. The central hypothesis of this dissertation was that selection due to the original site conditions of the natural inocula, and also, survival during fermentation likely favored isolates in the library that possess biofuel/bioenergy relevant traits (e.g. hydrolysis of lignocellulosic biomass, utilization of hydrolysis products, and tolerance to inhibitory compounds released during hydrolysis). The phylogeny for this library was used to identify and prioritize a diverse subsample of the library (n=207) for high-throughput screens of extracellular cellulase activity, n-butanol tolerance, vanillin utilization, and lignin degradation, as indicated by decolorization of the surrogate Congo red. Many isolates exhibited the capacities screened, including several isolates that were positive for more than one of these traits. Subsequently, a subset of the 207 screened isolates were studied further for tolerance and/or utilization of bioprocess byproducts (e.g. lignocellulosic hydrolysate, bio-oil) that are known to harbor both compounds with inhibitory effects on growth and pentose sugars released after hydrolysis. Polyhydroxyalkanoates (PHA) are bio-plastics produced by some bacteria. There is interest in industry in the identification of bacteria that can utilize wastes from some bioprocesses while creating bio-plastics, thus, a subset of the library (n=43 isolates) was screened for PHA accumulation associated with growth with the bio-diesel byproduct glycerol as the carbon source. Several isolates from the library are PHA producers with glycerol based on a fluorescence screen conducted. Microfluidic microbial fuel cells (MFCs) take advantage of microbial metabolism to convert organic matter to electricity. Microbial communities collected directly from soils were screened in a MFC array developed at Texas A&M. The screening of natural microbial communities identified electricigens with enhanced power generation abilities. The variation identified in these industrially relevant traits across isolates provides a proof of concept for both the existence of this variation in nature and the efficacy of employing fermentation and culturing approaches to enrich for these phenotypes. Also,… Advisors/Committee Members: Wilkinson, Heather (advisor), Kao, Katy (committee member), de Figueiredo, Paul (committee member), Yuan, Shuhua (committee member).

Subjects/Keywords: Bacillus; High-Throughput Screening; Biofuels; Bacterial Library; Environmental Isolates

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APA (6^th Edition):

Haynes, A. R. (2014). Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/152602

Chicago Manual of Style (16^th Edition):

Haynes, Abria R. “Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries.” 2014. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/152602.

MLA Handbook (7^th Edition):

Haynes, Abria R. “Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries.” 2014. Web. 19 Jan 2021.

Vancouver:

Haynes AR. Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/152602.

Council of Science Editors:

Haynes AR. Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/152602

Texas A&M University

29. LaiHing, Steven 1983-. Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis.

Degree: MS, Biochemistry, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/153195

► Mycobacterium tuberculosis currently affects 1/3 of the world's population. Over the past 20 years tuberculosis has become more resistant to all front line drugs used… (more)

Subjects/Keywords: HTS; TB; MTB; Drug Development and Design; High Throughput Screening; Tuberculosis

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APA (6^th Edition):

LaiHing, S. 1. (2011). Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153195

Chicago Manual of Style (16^th Edition):

LaiHing, Steven 1983-. “Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis.” 2011. Masters Thesis, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/153195.

MLA Handbook (7^th Edition):

LaiHing, Steven 1983-. “Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis.” 2011. Web. 19 Jan 2021.

Vancouver:

LaiHing S1. Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis. [Internet] [Masters thesis]. Texas A&M University; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/153195.

Council of Science Editors:

LaiHing S1. Using High Throughput Screening to Acquire Promising Drug Candidates Against Mycobacterium tuberculosis. [Masters Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/153195

Texas A&M University

30. Kim, Hyun Soo. High-throughput Microfluidic Screening Platforms for Microalgae Study.

Degree: PhD, Electrical Engineering, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/154060

► Microalgae have been envisioned as a future source of renewable energy. Both fossil fuel depletion and environmental concern have drawn more interest in microalgal biofuels,… (more)

▼ Microalgae have been envisioned as a future source of renewable energy. Both fossil fuel depletion and environmental concern have drawn more interest in microalgal biofuels, but the production cost of these biofuels are not yet economically competitive. Significant improvements such as development of better performing microalgal strains, optimization of culture conditions, and better understanding of microalgal biology are required for commercial viability. To resolve these limitations, massively parallel studies are needed, however, current microalgae culture systems are lack of high-throughput screening capabilities, and thus not suitable for the parallel studies. Here, three different high-throughput microfluidic microalgae screening platforms have been developed, each of which addresses major bottlenecks towards economically feasible microalgal biofuel. The first platform, a high-throughput microfluidic photobioreactor array has been developed to investigate the effect of different culture conditions on microalgal growth and oil production. This platform can provide up to 64 different culture conditions on-chip, such as combinations of different light intensities, light cycles, and culture media/chemical compositions. Single cell/colony trapping sites in culture compartments allowed for long-term analysis of microalgal growth and oil production with single cell/colony resolution. The light conditions that induced 1.8-fold higher oil accumulation over the typically used culture conditions were successfully identified. The second platform, as a microalgae library screening tool, a high-throughput microfluidic single-cell screening and selection platform has been developed to examine growth and oil production of various microalgal strains, followed by selective extraction of particular microalgae showing desired traits to off-chip reservoirs for further analysis. Single microalga was isolated and cultured, and its growth and oil accumulation were analyzed through 1024 single-cell trapping/culturing sites in the platform, where opening and closing of each trap can be individually controlled with integrated microfluidic control layers. By opening only a specific site out of the 1024 trapping sites, microalgae in particular trapping sites were selectively released and successfully collected off-chip. The third platform, a high-throughput droplet microfluidics-based microalgae screening platform has been developed to investigate the growth and the oil production of microalgal libraries with much higher throughput. Growth was characterized by encapsulating a single microalga into a droplet (functions as an independent bioreactor) and tracking its behavior over time. Oil production was also quantified through on-chip staining process, the key feature of the platform, where oil content in microalgae can be stained and measured through on-chip fluorescent tagging. Growth and oil accumulation under different culture conditions were successfully analyzed and compared, demonstrating the capability of the platform as a… Advisors/Committee Members: Han, Arum (advisor), Devarenne, Timothy (committee member), Yoon, Byung-Jun (committee member), Kameoka, Jun (committee member).

Subjects/Keywords: Microfluidic screening platform; Microalgae; High-throughput; Biofuel; Single cell analysis; Droplet

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APA (6^th Edition):

Kim, H. S. (2014). High-throughput Microfluidic Screening Platforms for Microalgae Study. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/154060

Chicago Manual of Style (16^th Edition):

Kim, Hyun Soo. “High-throughput Microfluidic Screening Platforms for Microalgae Study.” 2014. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/154060.

MLA Handbook (7^th Edition):

Kim, Hyun Soo. “High-throughput Microfluidic Screening Platforms for Microalgae Study.” 2014. Web. 19 Jan 2021.

Vancouver:

Kim HS. High-throughput Microfluidic Screening Platforms for Microalgae Study. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/154060.

Council of Science Editors:

Kim HS. High-throughput Microfluidic Screening Platforms for Microalgae Study. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/154060

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Open Access Theses and Dissertations