subject:(Heterogeneous nuclear ribonucleoproteins)

University of Texas Southwestern Medical Center

1. Melton, Alexis Allyson. Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF.

Degree: 2007, University of Texas Southwestern Medical Center

► CD45 is a hematopoetic-specific tyrosine phoshatase. In resting T cells three variable exons are partially repressed, and following antigen challenge, these exons are more highly… (more)

Subjects/Keywords: Heterogeneous-Nuclear Ribonucleoproteins; RNA Splicing; Repressor Proteins

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APA (6^th Edition):

Melton, A. A. (2007). Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Melton, Alexis Allyson. “Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF.” 2007. Thesis, University of Texas Southwestern Medical Center. Accessed March 07, 2021. http://hdl.handle.net/2152.5/662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Melton, Alexis Allyson. “Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF.” 2007. Web. 07 Mar 2021.

Vancouver:

Melton AA. Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2007. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2152.5/662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Melton AA. Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF. [Thesis]. University of Texas Southwestern Medical Center; 2007. Available from: http://hdl.handle.net/2152.5/662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Quaresma, Alexandre Jose Christino. Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1: Funcional and structural studies of human protein hnRNPQ.

Degree: 2008, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314763

► Abstract: The members of the hnRNPs family (heterogenous nuclear ribonuclein proteins) play important roles in gene expression control and mRNAs metabolism. The proteins hnRNPD (AUF1)… (more)

▼ Abstract: The members of the hnRNPs family (heterogenous nuclear ribonuclein proteins) play important roles in gene expression control and mRNAs metabolism. The proteins hnRNPD (AUF1) and hnRNPQ (NSAP1) were the main targets of this study. AUF1 has two RNA recognition motifs (RRM) and participates in the process of destabilization of a class of mRNAs that contain AU-rich sequences in their 3' untranslated regions (3'-UTR). We found, using the ¿yeast two-hybrid system¿ (Y2HS), that the isoform p37 of AUF1 (AUF1p37) interacts with the proteins: hnRNPQ, IMP-2, NSEP1 (YB-1) and UBC9. Moreover, the protein hnRNPQ was also identified as a prey protein in another Y2HS screen, which used as bait the human protein Arginine methyltransferase (PRMT1). HnRNPQ presents, in its C-terminal region, an "Arginine/Glicine-rich sequence" (RGG box). We are able to show that this RGG box is a target for methylation by PRMT1 in vitro and is methylated in vivo. Functionally, this methylation is important for its nuclear localization. hnRNPQ has a modular organization with an acid domain (AcD) in its N-terminal, followed by three RNA-binding domains (RRM) and the previously mentioned RGG box in its C-terminal. Functionally, hnRNPQ is involved in diverse aspects of RNA metabolism, including editing of the mRNA encoding the human protein ApoB. It has been shown previously to interact with the mRNA of ApoB, and also with the editing enzyme Apobec1 and the Apobec1 activation protein (ACF1). Here we show that the acid domain of hnRNPQ mediates the interaction with Apobec1 and that its in vitro phosphorylation (by PKC) inhibits this interaction. Furthermore, we found that hnRNPQ interacts with members the heat shock family of proteins (including HSP70 and BiP), and demonstrated that hnRNPQ can be in vitro phosphorylated by PKCd. Finally, we discovered that the sub-cellular localization of hnRNPQ undergoes modification after activation of PKC pathways. This also occurs after application of endoplasmic reticulum stress (using tarpsigargin), oxidative or heat stress. Under all of these conditions hnRNPQ translocated from the nucleus to the cytoplasm, where it is found at defined vesicles or granules. In summary, our data suggest that the diverse functions of hnRNPQ in the context of mRNA metabolism, may suffer specific regulations, by post-translational modifications, including phosphorylation and methylation, which modify both the proteins sub-cellular localizations as well as its affinity to interacting protein partners Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Kobarg, Jörg, 1965- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Funcional e Molecular (nameofprogram), Slovic, Avram Michael (committee member), Valentini, Sandro Roberto (committee member), Goldaman, Gustavo Henrique (committee member), Cerutti, Janete Maria (committee member).

Subjects/Keywords: Proteínas - Análise; Ribonucleoproteinas nucleares heterogeneas; RNA mensageiro; Proteins - Analysis; Heterogeneous-nuclear ribonucleoproteins; RNA, Messenger

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APA (6^th Edition):

Quaresma, A. J. C. (2008). Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1: Funcional and structural studies of human protein hnRNPQ. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314763

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Quaresma, Alexandre Jose Christino. “Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1: Funcional and structural studies of human protein hnRNPQ.” 2008. Thesis, Universidade Estadual de Campinas. Accessed March 07, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314763.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Quaresma, Alexandre Jose Christino. “Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1: Funcional and structural studies of human protein hnRNPQ.” 2008. Web. 07 Mar 2021.

Vancouver:

Quaresma AJC. Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1: Funcional and structural studies of human protein hnRNPQ. [Internet] [Thesis]. Universidade Estadual de Campinas; 2008. [cited 2021 Mar 07]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314763.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Quaresma AJC. Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1: Funcional and structural studies of human protein hnRNPQ. [Thesis]. Universidade Estadual de Campinas; 2008. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314763

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kyoto University

3. Al, Ismail Azza Darwish. Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination .

Degree: 2018, Kyoto University

URL: http://hdl.handle.net/2433/232307

Subjects/Keywords: Heterogeneous nuclear ribonucleoproteins; Hyper IgM syndrome; CRISPR/Cas9 mutagenesis; CH12F3-2A B cell; AID-deficient spleen cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Al, I. A. D. (2018). Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/232307

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Al, Ismail Azza Darwish. “Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination .” 2018. Thesis, Kyoto University. Accessed March 07, 2021. http://hdl.handle.net/2433/232307.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Al, Ismail Azza Darwish. “Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination .” 2018. Web. 07 Mar 2021.

Vancouver:

Al IAD. Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination . [Internet] [Thesis]. Kyoto University; 2018. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2433/232307.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Al IAD. Depletion of recombination-specific cofactors by the C-terminal mutant of the activation-induced cytidine deaminase causes the dominant negative effect on class switch recombination . [Thesis]. Kyoto University; 2018. Available from: http://hdl.handle.net/2433/232307

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

4. Nykamp, Keith Robert, 1974-. Coordination of MRNA 3' end formation and nuclear export by a nuclear poly(A)-Binding protein.

Degree: 2003, University of Florida

URL: https://ufdc.ufl.edu/AA00025840

Subjects/Keywords: DNA; Exons; Heterogeneous nuclear ribonucleoproteins; In vitro fertilization; Messenger RNA; Polyadenylation; Proteins; RNA; Splicing; Yeasts; Department of Molecular Genetics and Microbiology thesis Ph.D ( mesh ); Heterogeneous-Nuclear Ribonucleoproteins – genetics ( mesh ); Polyadenylation – genetics ( mesh ); RNA Polymerase II – genetics ( mesh ); RNA, Messenger – genetics ( mesh ); RNA-Binding Proteins – genetics ( mesh ); Research ( mesh ); Yeasts ( mesh )

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APA (6^th Edition):

Nykamp, Keith Robert, 1. (2003). Coordination of MRNA 3' end formation and nuclear export by a nuclear poly(A)-Binding protein. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00025840

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nykamp, Keith Robert, 1974-. “Coordination of MRNA 3' end formation and nuclear export by a nuclear poly(A)-Binding protein.” 2003. Thesis, University of Florida. Accessed March 07, 2021. https://ufdc.ufl.edu/AA00025840.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nykamp, Keith Robert, 1974-. “Coordination of MRNA 3' end formation and nuclear export by a nuclear poly(A)-Binding protein.” 2003. Web. 07 Mar 2021.

Vancouver:

Nykamp, Keith Robert 1. Coordination of MRNA 3' end formation and nuclear export by a nuclear poly(A)-Binding protein. [Internet] [Thesis]. University of Florida; 2003. [cited 2021 Mar 07]. Available from: https://ufdc.ufl.edu/AA00025840.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nykamp, Keith Robert 1. Coordination of MRNA 3' end formation and nuclear export by a nuclear poly(A)-Binding protein. [Thesis]. University of Florida; 2003. Available from: https://ufdc.ufl.edu/AA00025840

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Κορύλλου, Αγγελική. Μελέτη του μηχανισμού του κυτταρικού θανάτου που επάγεται από μεθυλιωτικούς παράγοντες.

Degree: 2009, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/24289

►

The elucidation of the molecular mechanism implicated in cell death following DNA damage induced by anticancer genotoxic agents is crucial for the improvement of chemotherapy… (more)

▼

The elucidation of the molecular mechanism implicated in cell death following DNA damage induced by anticancer genotoxic agents is crucial for the improvement of chemotherapy effectiveness. Methylating agents constitute a widely used class of anticancer drugs. They target DNA inducing DNA methylation adducts, the most biologically significant being O6-methylguanine (O6-meG) that is repaired by the specific repair enzyme O6-methylguanine DNA methyltransferase (MGMT). Furthermore, there is growing evidence that the molecular mechanism of methylating agent-induced apoptosis and cell death, not fully elucidated yet, is cell type dependent. RNA binding proteins constitute an abundant superfamily of multifunctional proteins. Certain members of this family (specifically hnRNPs) exhibit alterations of their expression during carcinogenesis, a fact related for them being considered as tumour markers. The aim of the present study was to investigate the effect of SΝ1 methylating agents on the cell cycle and on the induced cell death and to examine possible alterations occurring on RNA binding proteins during methylating agent-induced cell death in order to assess their possible use as markers of tumour resistance/sensitivity to chemotherapy. Human cell lines of different origin with varying expression levels of the DNA repair enzyme MGMT, were treated with isotoxic concentrations of N-methyl-N-nitrosourea (MNU), a model SN1 methylating agent. The mechanism of MNU-induced cell death was investigated along with possible modifications of hnRNP proteins. In an MGMT-inducible HeLa Tet-On clonal cell line (cervical carcinoma), cell cycle analysis revealed G2/M arrest followed by induction of apoptosis and dose dependent cell cycle alterations only in cells lacking functional MGMT. Molecular analysis revealed caspase-7 activation and PARP cleavage at late time points. Activation of the mitochondrial pathway in this system was supported by bcl-2 decline and caspase-9 cleavage. Alterations of hnRNP proteins (e.g. C1/C2) were observed upon induction of apoptosis. In A549 (non-small cell lung adenocarcinoma, p53wt) cells, MNU induced G2/M arrest, accompanied by cdc25A degradation, hnRNP B1 induction, hnRNP C1/C2 downregulation. The above findings along with those of the confocal microscopy, which revealed formation of multinucleated cells upon MNU treatment, imply mitotic catastrophe as the mode of cell death. Non-apoptotic cell death was confirmed by the lack of increase in the sub-G1 DNA content, PARP cleavage and caspase-3, -7 activation. In contrast, in H157 (non-small cell lung squamous carcinoma p53null) cells, MNU induced apoptotic cell death, confirmed by confocal microscopy (presence of apoptotic nuclei) cytofluorometry of DNA content and immunodetection of apoptotic markers, accompanied by overexpression of hnRNP B1 and C1/C2. Thus, the mechanism of the cell death induced by SN1 methylating agents is cell type-dependent and must be assessed prior cancer treatment.

Η αποσαφήνιση του μοριακού μηχανισμού…

Subjects/Keywords: Μεθυλιωτικοί παράγοντες SN1 τύπου; Κυτταρικός θάνατος; DNA μεθυλοτρανσφεράση της O6 μεθυλογουανίνης; Σηματοδότηση από βλάβη στο DNA; Ετερογενείς Πυρηνικές Ριβονουκλεοπρωτεΐνες (hnRNPs); SN1 methylating agents; Cell death; O6-methylguanine - DNA methyltransferase ( MGMT); DNA damage signalling; Heterogeneous nuclear ribonucleoproteins ( hnRNPs)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Κορύλλου, . �. (2009). Μελέτη του μηχανισμού του κυτταρικού θανάτου που επάγεται από μεθυλιωτικούς παράγοντες. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/24289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Κορύλλου, Αγγελική. “Μελέτη του μηχανισμού του κυτταρικού θανάτου που επάγεται από μεθυλιωτικούς παράγοντες.” 2009. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 07, 2021. http://hdl.handle.net/10442/hedi/24289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Κορύλλου, Αγγελική. “Μελέτη του μηχανισμού του κυτταρικού θανάτου που επάγεται από μεθυλιωτικούς παράγοντες.” 2009. Web. 07 Mar 2021.

Vancouver:

Κορύλλου �. Μελέτη του μηχανισμού του κυτταρικού θανάτου που επάγεται από μεθυλιωτικούς παράγοντες. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10442/hedi/24289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Κορύλλου �. Μελέτη του μηχανισμού του κυτταρικού θανάτου που επάγεται από μεθυλιωτικούς παράγοντες. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. Available from: http://hdl.handle.net/10442/hedi/24289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

6. Krecic, Annette Marie, 1968-. Nuclear and cytoplasmic functions of a yeast heterogeneous nuclear ribonucleoprotein.

Degree: 1998, University of Florida

URL: https://ufdc.ufl.edu/AA00047751

Subjects/Keywords: Cytoplasm; Heterogeneous nuclear ribonucleoproteins; In vitro fertilization; Messenger RNA; Polyadenylation; Proteins; RNA; Signals; Splicing; Yeasts; Amino Acid Sequence ( mesh ); Base Sequence ( mesh ); Cell Nucleus ( mesh ); Cytoplasm ( mesh ); Department of Molecular Genetics and Microbiology thesis Ph.D ( mesh ); Endonucleases – physiology ( mesh ); Molecular Sequence Data ( mesh ); RNA Precursors ( mesh ); RNA Processing, Post-Transcriptional – physiology ( mesh ); RNA Splicing – physiology ( mesh ); RNA, Messenger ( mesh ); Research ( mesh ); Ribonucleoproteins – genetics ( mesh ); Ribonucleoproteins – physiology ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krecic, Annette Marie, 1. (1998). Nuclear and cytoplasmic functions of a yeast heterogeneous nuclear ribonucleoprotein. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00047751

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krecic, Annette Marie, 1968-. “Nuclear and cytoplasmic functions of a yeast heterogeneous nuclear ribonucleoprotein.” 1998. Thesis, University of Florida. Accessed March 07, 2021. https://ufdc.ufl.edu/AA00047751.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krecic, Annette Marie, 1968-. “Nuclear and cytoplasmic functions of a yeast heterogeneous nuclear ribonucleoprotein.” 1998. Web. 07 Mar 2021.

Vancouver:

Krecic, Annette Marie 1. Nuclear and cytoplasmic functions of a yeast heterogeneous nuclear ribonucleoprotein. [Internet] [Thesis]. University of Florida; 1998. [cited 2021 Mar 07]. Available from: https://ufdc.ufl.edu/AA00047751.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krecic, Annette Marie 1. Nuclear and cytoplasmic functions of a yeast heterogeneous nuclear ribonucleoprotein. [Thesis]. University of Florida; 1998. Available from: https://ufdc.ufl.edu/AA00047751

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

7. Hector, Ronald Earl, 1972-. Posttranscriptional regulation of gene expression by a nuclear polyadenylated RNA binding protein.

Degree: 2000, University of Florida

URL: https://ufdc.ufl.edu/AA00038398

Subjects/Keywords: Heterogeneous nuclear ribonucleoproteins; In vitro fertilization; Introns; Messenger RNA; Nucleotides; Polyadenylation; RNA; Small nucleolar RNA; Splicing; Yeasts; Department of Molecular Genetics and Microbiology thesis Ph.D ( mesh ); Gene Expression Regulation ( mesh ); Genetic Techniques ( mesh ); Nuclear Proteins ( mesh ); RNA Precursors ( mesh ); RNA, Messenger ( mesh ); RNA-Binding Proteins ( mesh ); Research ( mesh ); City of Gainesville ( local )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hector, Ronald Earl, 1. (2000). Posttranscriptional regulation of gene expression by a nuclear polyadenylated RNA binding protein. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00038398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hector, Ronald Earl, 1972-. “Posttranscriptional regulation of gene expression by a nuclear polyadenylated RNA binding protein.” 2000. Thesis, University of Florida. Accessed March 07, 2021. https://ufdc.ufl.edu/AA00038398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hector, Ronald Earl, 1972-. “Posttranscriptional regulation of gene expression by a nuclear polyadenylated RNA binding protein.” 2000. Web. 07 Mar 2021.

Vancouver:

Hector, Ronald Earl 1. Posttranscriptional regulation of gene expression by a nuclear polyadenylated RNA binding protein. [Internet] [Thesis]. University of Florida; 2000. [cited 2021 Mar 07]. Available from: https://ufdc.ufl.edu/AA00038398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hector, Ronald Earl 1. Posttranscriptional regulation of gene expression by a nuclear polyadenylated RNA binding protein. [Thesis]. University of Florida; 2000. Available from: https://ufdc.ufl.edu/AA00038398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Παπαδοπούλου, Χριστίνα. Διερεύνηση του ρόλου πρωτεϊνών µε ικανότητα πρόσδεσης hnRNA και mRNA στην επαγωγή, εξέλιξη και καθιέρωση του καρκίνου του πνεύµονα σε πειραµατικά πρότυπα καρκινογένεσης.

Degree: 2009, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/17785

► The present dissertation refers to the involvement of RNA binding proteins (RBPs) in the induction and establishment of lung cancer in cellular and animal experimental… (more)

▼ The present dissertation refers to the involvement of RNA binding proteins (RBPs) in the induction and establishment of lung cancer in cellular and animal experimental models. The study has focused on the specific role of the heterogeneous nuclear ribonucleoproteins family (hnRNP), with major emphasis in hnRNPs A/B type (hnRNP A1, A2/B1, A3), stable components of hnRNP and mRNP complexes. which participate in almost all cellular processes related to the biogenesis of mRNA. In parallel, the expression pattern of other RBP proteins was studied, with emphasis in HuR protein that has a major role in the stability/translation of mRNA targets. Since the biological significance of the involvment of hnRNP (mainly A2/B1) and HuR proteins in the human lung cancer is almost established, we studied experimental mouse models of chemically induced lung cancer. Overall, three different mouse strains and two schemes of chemical lung cancer induction [urethane and aromatic polycyclic hydrocarbons (PAH)] were applied. Immunochemical detection of the expression levels of RBP proteins was performed in total extracts prepared from lung tissue as well as histological/immunohistochemical analysis. The results from the three experimental mouse models are compatible with the absence of a detectable increase in the levels of the proteins tested in lung cancer tissue compared to normal tissue samples. These findings in principle agree with current data reported by Peebles KA et al (2007), showing changes in the levels of hnRNP A2/B1. Therefore, the results obtained from the application of experimental mouse models are in obvious disagreement with those from human lung cancer biopsies, where overexpression of hnRNP A/B and HuR occurs in high proportion as compared to the adjacent normal tissue. Therefore, it is recommended that these results should be taken into consideration before applying outcome animal models in the human lung cancer. Based on the common characteristics that hnRNP A/B and HuR proteins share, we proceeded in the identification and characterization of putative interactions between these proteins by performing a series of immunoprecipitation assays in nuclear and cytoplasmic fractions derived from A549 cells (human lung adenocarcinoma). We succeeded in identifying a broad spectrum of HuR interactions within hnRNP and mRNP complexes. More specifically, nuclear interactions of hnRNP A1:HuR and hnRNP C1/C2:HuR , as well as cytoplasmic hnRNPA1:HuR interactions were characterized. These associations refer to protein/RNA interactions. In addition, the ability of HuR to make protein/protein interactions with the C-terminus of hnRNP A3, was characterized in vitro. Heat shock treatment of A549 cells modified the nuclear distribution of HuR and affected the interaction of HuR with hnRNP complexes. These findings may lead to the identification of the mRNA in the hnRNP Α1:HuR immunocomplex, in cells with or without HuR knockout, in order to investigate the biological significance of the presence of HuR in hnRNP and mRNP…

Subjects/Keywords: Πρωτεΐνες µε ικανότητα πρόσδεσης RNA; Ετερογενείς Πυρηνικές Ριβονουκλεοπρωτεΐνες (hnRNPs); Καρκίνος πνεύμονα; Καρκινογένεση πνεύµονα ποντικού; Πρωτεϊνη HuR; Πρωτεΐνες, ?ιασυνδέσεις µεταξύ; Ανοσοϊστοχημεία; Ανοσοκατακρήμνιση; RNA binding proteins; Heterogeneous nuclear ribonucleoproteins ( hnRNPs); Lung cancer; Mouse lung carcinogenesis; HuR prottein; Protein - RNA interactions; Immunohistochemistry ( IHC)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Παπαδοπούλου, . �. (2009). Διερεύνηση του ρόλου πρωτεϊνών µε ικανότητα πρόσδεσης hnRNA και mRNA στην επαγωγή, εξέλιξη και καθιέρωση του καρκίνου του πνεύµονα σε πειραµατικά πρότυπα καρκινογένεσης. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/17785

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Παπαδοπούλου, Χριστίνα. “Διερεύνηση του ρόλου πρωτεϊνών µε ικανότητα πρόσδεσης hnRNA και mRNA στην επαγωγή, εξέλιξη και καθιέρωση του καρκίνου του πνεύµονα σε πειραµατικά πρότυπα καρκινογένεσης.” 2009. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed March 07, 2021. http://hdl.handle.net/10442/hedi/17785.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Παπαδοπούλου, Χριστίνα. “Διερεύνηση του ρόλου πρωτεϊνών µε ικανότητα πρόσδεσης hnRNA και mRNA στην επαγωγή, εξέλιξη και καθιέρωση του καρκίνου του πνεύµονα σε πειραµατικά πρότυπα καρκινογένεσης.” 2009. Web. 07 Mar 2021.

Vancouver:

Παπαδοπούλου �. Διερεύνηση του ρόλου πρωτεϊνών µε ικανότητα πρόσδεσης hnRNA και mRNA στην επαγωγή, εξέλιξη και καθιέρωση του καρκίνου του πνεύµονα σε πειραµατικά πρότυπα καρκινογένεσης. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2009. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10442/hedi/17785.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Παπαδοπούλου �. Διερεύνηση του ρόλου πρωτεϊνών µε ικανότητα πρόσδεσης hnRNA και mRNA στην επαγωγή, εξέλιξη και καθιέρωση του καρκίνου του πνεύµονα σε πειραµατικά πρότυπα καρκινογένεσης. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2009. Available from: http://hdl.handle.net/10442/hedi/17785

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Μπουκάκης, Γεώργιος. Μελέτη του βιολογικού ρόλου πρωτεϊνών με ικανότητα πρόσδεσης hnRNA και mRNA καθώς και των μηχανισμών της απορύθμισης τους στον ανθρώπινο καρκίνο του πνεύμονα.

Degree: 2009, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/18145

► The main goal of the present Thesis was to determine the molecular characteristics of the deregulated expression of RNA binding proteins (RBPs), in the human… (more)

▼ The main goal of the present Thesis was to determine the molecular characteristics of the deregulated expression of RNA binding proteins (RBPs), in the human lung cancer, in paired biopsies taken from cancerous and normal adjacent tissue from the same patient. The study focused in members of hnRNP proteins family belonging to the hnRNP A/B subtype (A1, A2/B1 and A3), along with other hnRNPs (hnRNP K/J) as well as the splicing factor ASF/SF2 with a known antagonistic role to hnRNPs in the alternative splicing. In total, 21 biopsy pairs were examined from patients with non-small cell lung cancer (NSCLC) (in collaboration with Dr.Ch. Valavanis, “Metaxa” hospital, in the frame of the PENED program). Western blotting and quantitative Real-Time PCR were applied for the semi-quantitative estimation of protein levels in combination with the quantitive estimation of mRNA levels, respectively. This approach allowed the direct comparison of protein and mRNA patterns of expression in cancer/normal tissue from the same patient. To summarize the results, the comparison of cancer related alterations of hnRNP proteins and ASF/SF2 pointed to the lack of correlation between protein and mRNA levels, with the exception of the hnRNP A2/B1. Moreover, the level of overexpression detected for hnRNP A1 and ASF/SF2 in the cancerous tissue, did not correlate with changes in the mRNA level. The results support the existence of distinct molecular mechanisms regulating the expression of closely related hnRNP A/B subtype proteins. In the course of this project new tools, useful in study of hnRNPs, were produced, refering to the expression of recombinant hnRNP A2 and A3 proteins. The application of these new tools, in a series of pull-down experiments performed in lung cell lines extracts, led to the identification of new interactions among hnRNP proteins, in hnRNP and mRNP complexes. The use of these tools also allowed us to investigate the possibility of establishing the hnRNP proteins as biomarker for the early detection of lung cancer. Experiments applying the recombinant proteins hnRNP A/B, as specific antigens, gave clear evidence for the presence of autoantibodies targeting those proteins, in the blood serum of patients with cancer, a finding that rises the perspective of using these proteins, in combination with other biomarkers, as a reliable marker for malignant detection in early stages of lung cancer.

Subjects/Keywords: Καρκίνος πνεύμονα; Ετερογενής πυρηνικές ριβονουκλεοπρωτεΐνες; Μετα-μεταγραφικός έλεγχος; Βιολογικοί δείκτες; Επίπεδα πρωτεϊνών / RNA; Υπερέκφραση πρωτεϊνών; Παράγοντας ματίσματος; Αυτοαντισώματα; Lung cancer; Heterogeneous nuclear ribonucleoproteins ( hnRNPs); Post-transcriptional control; Biological markers; Protein/mRNA levels; Protein over-expression; Splicing regulator; Autoantibodies

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Μπουκάκης, . �. (2009). Μελέτη του βιολογικού ρόλου πρωτεϊνών με ικανότητα πρόσδεσης hnRNA και mRNA καθώς και των μηχανισμών της απορύθμισης τους στον ανθρώπινο καρκίνο του πνεύμονα. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/18145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Μπουκάκης, Γεώργιος. “Μελέτη του βιολογικού ρόλου πρωτεϊνών με ικανότητα πρόσδεσης hnRNA και mRNA καθώς και των μηχανισμών της απορύθμισης τους στον ανθρώπινο καρκίνο του πνεύμονα.” 2009. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed March 07, 2021. http://hdl.handle.net/10442/hedi/18145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Μπουκάκης, Γεώργιος. “Μελέτη του βιολογικού ρόλου πρωτεϊνών με ικανότητα πρόσδεσης hnRNA και mRNA καθώς και των μηχανισμών της απορύθμισης τους στον ανθρώπινο καρκίνο του πνεύμονα.” 2009. Web. 07 Mar 2021.

Vancouver:

Μπουκάκης �. Μελέτη του βιολογικού ρόλου πρωτεϊνών με ικανότητα πρόσδεσης hnRNA και mRNA καθώς και των μηχανισμών της απορύθμισης τους στον ανθρώπινο καρκίνο του πνεύμονα. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2009. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10442/hedi/18145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Μπουκάκης �. Μελέτη του βιολογικού ρόλου πρωτεϊνών με ικανότητα πρόσδεσης hnRNA και mRNA καθώς και των μηχανισμών της απορύθμισης τους στον ανθρώπινο καρκίνο του πνεύμονα. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2009. Available from: http://hdl.handle.net/10442/hedi/18145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

10. Miller, Jill Watkins. Human triplet repeat RNA-binding proteins involvement in mRNA biogenesis and genetic disease.

Degree: 1998, University of Florida

URL: https://ufdc.ufl.edu/AA00031573

Subjects/Keywords: Diseases; Hela cells; Heterogeneous nuclear ribonucleoproteins; Messenger RNA; Myotonic dystrophy; Polyadenylation; RNA; RNA binding proteins; Splicing; Yeasts; Department of Molecular Genetics and Microbiology thesis Ph.D ( mesh ); Models, Genetic ( mesh ); Mutation ( mesh ); Myotonic Dystrophy – etiology ( mesh ); Myotonic Dystrophy – genetics ( mesh ); RNA, Messenger – biosynthesis ( mesh ); RNA-Binding Proteins ( mesh ); Research ( mesh ); Transcription, Genetic ( mesh ); Trinucleotide Repeats ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miller, J. W. (1998). Human triplet repeat RNA-binding proteins involvement in mRNA biogenesis and genetic disease. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00031573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miller, Jill Watkins. “Human triplet repeat RNA-binding proteins involvement in mRNA biogenesis and genetic disease.” 1998. Thesis, University of Florida. Accessed March 07, 2021. https://ufdc.ufl.edu/AA00031573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miller, Jill Watkins. “Human triplet repeat RNA-binding proteins involvement in mRNA biogenesis and genetic disease.” 1998. Web. 07 Mar 2021.

Vancouver:

Miller JW. Human triplet repeat RNA-binding proteins involvement in mRNA biogenesis and genetic disease. [Internet] [Thesis]. University of Florida; 1998. [cited 2021 Mar 07]. Available from: https://ufdc.ufl.edu/AA00031573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miller JW. Human triplet repeat RNA-binding proteins involvement in mRNA biogenesis and genetic disease. [Thesis]. University of Florida; 1998. Available from: https://ufdc.ufl.edu/AA00031573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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