subject:(Heparin)

Oregon State University

1. Fry, Allyson Kaye. Synthesis and anticoagulant function of heparin containing block copolymers on polystyrene microspheres.

Degree: MS, Chemical Engineering, 2008, Oregon State University

URL: http://hdl.handle.net/1957/8926

► Contact of blood with the surfaces of synthetic materials is associated with spontaneous protein adsorption, initiating platelet aggregation, the coagulation cascade, and the eventual development… (more)

Subjects/Keywords: heparin; Heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fry, A. K. (2008). Synthesis and anticoagulant function of heparin containing block copolymers on polystyrene microspheres. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/8926

Chicago Manual of Style (16^th Edition):

Fry, Allyson Kaye. “Synthesis and anticoagulant function of heparin containing block copolymers on polystyrene microspheres.” 2008. Masters Thesis, Oregon State University. Accessed January 26, 2020. http://hdl.handle.net/1957/8926.

MLA Handbook (7^th Edition):

Fry, Allyson Kaye. “Synthesis and anticoagulant function of heparin containing block copolymers on polystyrene microspheres.” 2008. Web. 26 Jan 2020.

Vancouver:

Fry AK. Synthesis and anticoagulant function of heparin containing block copolymers on polystyrene microspheres. [Internet] [Masters thesis]. Oregon State University; 2008. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/1957/8926.

Council of Science Editors:

Fry AK. Synthesis and anticoagulant function of heparin containing block copolymers on polystyrene microspheres. [Masters Thesis]. Oregon State University; 2008. Available from: http://hdl.handle.net/1957/8926

University of Hong Kong

2. 劉泳欣; Lau, Wing-yan. Cloning and characterization of heparanase 2 in rat brain.

Degree: M. Phil., 2016, University of Hong Kong

URL: Lau, W. [劉泳欣]. (2016). Cloning and characterization of heparanase 2 in rat brain. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5736654. ; http://dx.doi.org/10.5353/th_b5736654 ; http://hdl.handle.net/10722/238186

►

Synaptic activity modulates the density of synaptic AMPARs, thereby affecting synaptic function, learning and memory. The molecular mechanisms regulating AMPAR trafficking is under intense interest.… (more)

▼

Synaptic activity modulates the density of synaptic AMPARs, thereby affecting synaptic function, learning and memory. The molecular mechanisms regulating AMPAR trafficking is under intense interest. Heparan sulfates (HS) of perineuronal proteoglycans are implicated in controlling the open-state of AMPA receptor-ion channels for maintaining synaptic strength. Heparanase 1 (Hpa1) is an endoglycosidase that cleaves heparan sulfate (HS) polysaccharides on HS proteoglycans (HSPGs). Its pro-form which is however enzymatically inactive induces internalization of HSPGs and AMPARs at post-synaptic terminal in neurons, resulting in decrease in synaptic strength and long-term potentiation (LTP). Its homolog, heparanase 2 (Hpa2), with about 40% homology is found expressed in the human brain, were found interacting with heparanase 1. We hypothesize that Hpa2 is expressed in rat brain and secreted from the hippocampal neurons, interact with hpa1 and regulate synaptic plasticity. In this project the expression, localization and secretion of Hpa2 were studied in rat brain. It is found heparanase 2 expression is highest among different distinct brain region. Hippocampal DIV14-16 neurons in culture from embryonic (E)17-18 rats and adult rat brain slices are adopted for localization analysis. Immunohistochemical staining on brain slice demonstrated that Hpa2 was localized at the CA1, CA3 and DG region in the hippocampus and Fix cell immunocytochemistry showed Hpa2 was co-localized with syndecan-3 in the nucleus, while in live cell staining hpa2 were found on cell surface co-localized with unknown HSPG. RT-PCR and Western blot analysis showed the presence of Hpa2 in hippocampal neurons at in In our hippocampal neuron culture model, Hpa2 is secreted from hippocampal neurons culture spontaneously. We pursue to study the signaling that regulates the neuronal production/secretion of Hpa2. Hpa2 exocytosis by the hippocampal neurons was enhanced by protein kinase C (PKC) activation and protein kinase A (PKA) inhibition. To figure out if hpa2 release is activity dependent, TTX and BIC were added to the hippocampal neuron cultures to induce synaptic scaling. Yet, hpa2 release and synthesis is not affected. In order to fully understand the function of hpa2, we aimed to produce recombinant hpa2. We discovered a new isoform of rat heparanase 2 which has 98% of similarity with human hpa2. The hpa2 isoform is successfully cloned and a stable expression cell line of hpa2 in HEK293 cell is developed. Taken together, the findings from the experiments support the hypothesis to show Hpa2 is expressed, localized and secreted in the rat hippocampal and hippocampal neurons. However, the function of heparanase 2 in hippocampus, especial its role in regulation of synaptic plasticity remain to be revealed.

published_or_final_version

Biomedical Sciences

Master

Master of Philosophy

Subjects/Keywords: Brain; Heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

劉泳欣; Lau, W. (2016). Cloning and characterization of heparanase 2 in rat brain. (Masters Thesis). University of Hong Kong. Retrieved from Lau, W. [劉泳欣]. (2016). Cloning and characterization of heparanase 2 in rat brain. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5736654. ; http://dx.doi.org/10.5353/th_b5736654 ; http://hdl.handle.net/10722/238186

Chicago Manual of Style (16^th Edition):

劉泳欣; Lau, Wing-yan. “Cloning and characterization of heparanase 2 in rat brain.” 2016. Masters Thesis, University of Hong Kong. Accessed January 26, 2020. Lau, W. [劉泳欣]. (2016). Cloning and characterization of heparanase 2 in rat brain. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5736654. ; http://dx.doi.org/10.5353/th_b5736654 ; http://hdl.handle.net/10722/238186.

MLA Handbook (7^th Edition):

劉泳欣; Lau, Wing-yan. “Cloning and characterization of heparanase 2 in rat brain.” 2016. Web. 26 Jan 2020.

Vancouver:

劉泳欣; Lau W. Cloning and characterization of heparanase 2 in rat brain. [Internet] [Masters thesis]. University of Hong Kong; 2016. [cited 2020 Jan 26]. Available from: Lau, W. [劉泳欣]. (2016). Cloning and characterization of heparanase 2 in rat brain. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5736654. ; http://dx.doi.org/10.5353/th_b5736654 ; http://hdl.handle.net/10722/238186.

Council of Science Editors:

劉泳欣; Lau W. Cloning and characterization of heparanase 2 in rat brain. [Masters Thesis]. University of Hong Kong; 2016. Available from: Lau, W. [劉泳欣]. (2016). Cloning and characterization of heparanase 2 in rat brain. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5736654. ; http://dx.doi.org/10.5353/th_b5736654 ; http://hdl.handle.net/10722/238186

McGill University

3. Helleur, Robert. Synthetic and structural studies related to the chemistry of heparin.

Degree: MS, Department of Chemistry, 1977, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile54082.pdf

Subjects/Keywords: Heparin.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Helleur, R. (1977). Synthetic and structural studies related to the chemistry of heparin. (Masters Thesis). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile54082.pdf

Chicago Manual of Style (16^th Edition):

Helleur, Robert. “Synthetic and structural studies related to the chemistry of heparin.” 1977. Masters Thesis, McGill University. Accessed January 26, 2020. http://digitool.library.mcgill.ca/thesisfile54082.pdf.

MLA Handbook (7^th Edition):

Helleur, Robert. “Synthetic and structural studies related to the chemistry of heparin.” 1977. Web. 26 Jan 2020.

Vancouver:

Helleur R. Synthetic and structural studies related to the chemistry of heparin. [Internet] [Masters thesis]. McGill University; 1977. [cited 2020 Jan 26]. Available from: http://digitool.library.mcgill.ca/thesisfile54082.pdf.

Council of Science Editors:

Helleur R. Synthetic and structural studies related to the chemistry of heparin. [Masters Thesis]. McGill University; 1977. Available from: http://digitool.library.mcgill.ca/thesisfile54082.pdf

University of Hong Kong

4. Wong, Cheuk-ning. Purification and characterization of recombinant heparanase 2.

Degree: Master of Medical Sciences, 2016, University of Hong Kong

URL: Wong, C. [黃卓寧]. (2016). Purification and characterization of recombinant heparanase 2. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5804938. ; http://dx.doi.org/10.5353/th_b5804938 ; http://hdl.handle.net/10722/238409

►

On mammalian cell surface, the role of extracellular matrix (ECM) is found to be crucial in providing structural support to cells. In addition, the ECM… (more)

▼

On mammalian cell surface, the role of extracellular matrix (ECM) is found to be crucial in providing structural support to cells. In addition, the ECM confers barrier property to nearby tissues and serves as a platform for growth factor binding and cell signaling from the extracellular to the intracellular environment. In developing as well as cancer tissue, modulation of the ECM regulates cell emigration versus homing. The ECM has also been implicated in the regulation of synaptic plasticity. The ECM is composed of a supramolecular array of proteoglycans and glycoproteins. Such a complex structure can be categorized according to function and/or structural components. In fact, the ECM is a dynamic structure in which the abundance and components are subject to change so as to meet physiological needs of cells and such modulation is usually accomplished via enzymes. In this dissertation, we focus on one of the ECM components, heparan sulfate (HS) proteoglycans and the HS-binding protein, heparanase 2, which show homology to heparanase 1 but lack HS-cleaving enzyme activity. Clinical and biochemical research studies have already proved that the heparanase 2 protein can attenuate the growth of cancer cells and tumor metastasis but the exact underlying mechanism remains unclear . It is hypothesized that it competes with its homolog heparanase 1 for binding to HS. When the HS binding sites are occupied by heparanase 2, enzymatic cleavage of the HS by heparanase 1 would be limited so that metastatic migration of cancer cells could be inhibited. However, this hypothesis is yet to be confirmed and some clinical studies of certain types of cancer have reported that the inhibition of cancer metastasis by heparanase 2 have no direct relation with heparanase 1 . Results from our lab have also proposed a model that the synaptic strength could be modulated through pro-heparanase /HSPG clustering of AMPAR in the peri-synapse resulting in AMPAR internalization. It is therefore to our interest to find if heparanase 2 also plays a role in such synaptic plasticity and if so, how this occurs. To address this, recombinant heparanase 2 protein was prepared and purified. With use of the recombinant protein preparation, this study demonstrated the binding affinity of heparanase 2 was stronger than its homolog heparanase 1 but heparanase 2 lacked HS-cleaving enzyme activity. This paves the way for further studies on the role of heparanase 2 in synaptic plasticity.

published_or_final_version

Medicine

Master

Master of Medical Sciences

Subjects/Keywords: Extracellular matrix; Heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, C. (2016). Purification and characterization of recombinant heparanase 2. (Masters Thesis). University of Hong Kong. Retrieved from Wong, C. [黃卓寧]. (2016). Purification and characterization of recombinant heparanase 2. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5804938. ; http://dx.doi.org/10.5353/th_b5804938 ; http://hdl.handle.net/10722/238409

Chicago Manual of Style (16^th Edition):

Wong, Cheuk-ning. “Purification and characterization of recombinant heparanase 2.” 2016. Masters Thesis, University of Hong Kong. Accessed January 26, 2020. Wong, C. [黃卓寧]. (2016). Purification and characterization of recombinant heparanase 2. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5804938. ; http://dx.doi.org/10.5353/th_b5804938 ; http://hdl.handle.net/10722/238409.

MLA Handbook (7^th Edition):

Wong, Cheuk-ning. “Purification and characterization of recombinant heparanase 2.” 2016. Web. 26 Jan 2020.

Vancouver:

Wong C. Purification and characterization of recombinant heparanase 2. [Internet] [Masters thesis]. University of Hong Kong; 2016. [cited 2020 Jan 26]. Available from: Wong, C. [黃卓寧]. (2016). Purification and characterization of recombinant heparanase 2. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5804938. ; http://dx.doi.org/10.5353/th_b5804938 ; http://hdl.handle.net/10722/238409.

Council of Science Editors:

Wong C. Purification and characterization of recombinant heparanase 2. [Masters Thesis]. University of Hong Kong; 2016. Available from: Wong, C. [黃卓寧]. (2016). Purification and characterization of recombinant heparanase 2. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5804938. ; http://dx.doi.org/10.5353/th_b5804938 ; http://hdl.handle.net/10722/238409

University of Debrecen

5. Timári, István. Heparin-analóg szénhidrátszármazékok szerkezetének és antitrombin -III fehérjével való kölcsönhatásának vizsgálata NMR spektroszkópiával .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Kémiai Intézet, 2012, University of Debrecen

URL: http://hdl.handle.net/2437/128597

► A heparin régóta ismert és alkalmazott véralvadásgátló poliszacharid az orvosi gyakorlatban. Több kutatócsoport is sikeresen előállított olyan heparin-analóg származékokat, amelyek a heparinhoz képest kedvezőbb farmakológiai… (more)

Subjects/Keywords: NMR spektroszkópia; heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Timári, I. (2012). Heparin-analóg szénhidrátszármazékok szerkezetének és antitrombin -III fehérjével való kölcsönhatásának vizsgálata NMR spektroszkópiával . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/128597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Timári, István. “Heparin-analóg szénhidrátszármazékok szerkezetének és antitrombin -III fehérjével való kölcsönhatásának vizsgálata NMR spektroszkópiával .” 2012. Thesis, University of Debrecen. Accessed January 26, 2020. http://hdl.handle.net/2437/128597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Timári, István. “Heparin-analóg szénhidrátszármazékok szerkezetének és antitrombin -III fehérjével való kölcsönhatásának vizsgálata NMR spektroszkópiával .” 2012. Web. 26 Jan 2020.

Vancouver:

Timári I. Heparin-analóg szénhidrátszármazékok szerkezetének és antitrombin -III fehérjével való kölcsönhatásának vizsgálata NMR spektroszkópiával . [Internet] [Thesis]. University of Debrecen; 2012. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/2437/128597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Timári I. Heparin-analóg szénhidrátszármazékok szerkezetének és antitrombin -III fehérjével való kölcsönhatásának vizsgálata NMR spektroszkópiával . [Thesis]. University of Debrecen; 2012. Available from: http://hdl.handle.net/2437/128597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Kevane, Barry. Novel Endothelial Protective and Anti-Thrombotic Effects of Therapeutic Agents in Malignant and Inflammatory Diseases: Molecular Mechanisms and Translational Relevance.

Degree: 2018, School of Medicine & Medical Science

URL: http://hdl.handle.net/10197/9535

►

While the primary physiological function of the blood coagulation system is to prevent bleeding following vascular injury, a complex interplay is known to exist between… (more)

▼

While the primary physiological function of the blood coagulation system is to prevent bleeding following vascular injury, a complex interplay is known to exist between components of the blood coagulation cascade and the activity of various other biological processes and systemic diseases including inflammation, tumour metastasis and pulmonary vascular disease. Moreover, the potential of this interplay to provide novel therapeutic opportunities is increasingly representing a source of scientific and translational interest, particularly in the setting of clinical scenarios where therapeutic options are limited, such as in the treatment and prevention of cancer metastasis and in the management of thrombotic risk among individuals at high risk of bleeding.The work described within this thesis was conducted with the aim of exploring the nature of this interplay in the context of two disease states, cancer metastasis and Eisenmenger syndrome, and to explore potential novel therapeutic strategies which target the interaction between coagulation activation and the underlying systemic disease in both cases. LMWH, an anticoagulant drug, appears to mediate an anti-metastatic effect in vivo but the underlying mechanisms remain to be elucidated and the associated risk of bleeding precludes its use in cancer solely for the prevention of tumour dissemination. Eisenmenger syndrome is associated with a significant risk of both haemorrhage and thrombosis, the underlying mechanisms remain poorly understood and the nature of the competing thrombotic and haemorrhagic risks presents significant clinical dilemmas.LMWH was found to support endothelial barrier function in vitro (a key barrier to tumour metastasis) and to inhibit tumour cell trans-endothelial migration. This activity does not appear to be linked to its anticoagulant function and both a non-anticoagulant LMWH fraction and a combination of low dose LMWH with a statin were found to represent a novel means of delivering the cytoprotective effects of LMWH in vitro in the absence of a significant anticoagulant effect.Abnormal platelet procoagulant activity was found to represent a key determinant of the prothrombotic phenotype in Eisenmenger syndrome but, interestingly, platelets were also found to modulate the activity of endogenous anticoagulant pathways in a manner which would be predicted to increase the bleeding risk (reflecting the clinical phenotype). Remarkably, following a course of treatment with macitentan, a dual endothelin-1 receptor antagonist which does not directly affect haemostasis, the derangements in both procoagulant and anticoagulant pathways were found to be attenuated.In summary, the cross-talk between coagulation and systemic disease represents a potential source of novel therapeutic opportunities. The in vitro results described in this thesis may, if replicated in vivo, represent a means of addressing significant clinical dilemmas in two high risk patient groups.

Check date.issued and date.embargo: Flexible delayed release embargo added by author

Advisors/Committee Members: Ní Áinle, Fionnuala.

Subjects/Keywords: Heparin; Metastasis; Thrombosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kevane, B. (2018). Novel Endothelial Protective and Anti-Thrombotic Effects of Therapeutic Agents in Malignant and Inflammatory Diseases: Molecular Mechanisms and Translational Relevance. (Thesis). School of Medicine & Medical Science. Retrieved from http://hdl.handle.net/10197/9535

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kevane, Barry. “Novel Endothelial Protective and Anti-Thrombotic Effects of Therapeutic Agents in Malignant and Inflammatory Diseases: Molecular Mechanisms and Translational Relevance.” 2018. Thesis, School of Medicine & Medical Science. Accessed January 26, 2020. http://hdl.handle.net/10197/9535.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kevane, Barry. “Novel Endothelial Protective and Anti-Thrombotic Effects of Therapeutic Agents in Malignant and Inflammatory Diseases: Molecular Mechanisms and Translational Relevance.” 2018. Web. 26 Jan 2020.

Vancouver:

Kevane B. Novel Endothelial Protective and Anti-Thrombotic Effects of Therapeutic Agents in Malignant and Inflammatory Diseases: Molecular Mechanisms and Translational Relevance. [Internet] [Thesis]. School of Medicine & Medical Science; 2018. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/10197/9535.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kevane B. Novel Endothelial Protective and Anti-Thrombotic Effects of Therapeutic Agents in Malignant and Inflammatory Diseases: Molecular Mechanisms and Translational Relevance. [Thesis]. School of Medicine & Medical Science; 2018. Available from: http://hdl.handle.net/10197/9535

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

7. Vercellotti, John Raymond. The structure of heparin.

Degree: PhD, Graduate School, 1963, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486553441669325

Subjects/Keywords: Chemistry; Heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vercellotti, J. R. (1963). The structure of heparin. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486553441669325

Chicago Manual of Style (16^th Edition):

Vercellotti, John Raymond. “The structure of heparin.” 1963. Doctoral Dissertation, The Ohio State University. Accessed January 26, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486553441669325.

MLA Handbook (7^th Edition):

Vercellotti, John Raymond. “The structure of heparin.” 1963. Web. 26 Jan 2020.

Vancouver:

Vercellotti JR. The structure of heparin. [Internet] [Doctoral dissertation]. The Ohio State University; 1963. [cited 2020 Jan 26]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486553441669325.

Council of Science Editors:

Vercellotti JR. The structure of heparin. [Doctoral Dissertation]. The Ohio State University; 1963. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486553441669325

The Ohio State University

8. Tomomatsu, Hideo. Structural studies on herapin.

Degree: PhD, Graduate School, 1965, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu14865679584659

Subjects/Keywords: Chemistry; Heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tomomatsu, H. (1965). Structural studies on herapin. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu14865679584659

Chicago Manual of Style (16^th Edition):

Tomomatsu, Hideo. “Structural studies on herapin.” 1965. Doctoral Dissertation, The Ohio State University. Accessed January 26, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu14865679584659.

MLA Handbook (7^th Edition):

Tomomatsu, Hideo. “Structural studies on herapin.” 1965. Web. 26 Jan 2020.

Vancouver:

Tomomatsu H. Structural studies on herapin. [Internet] [Doctoral dissertation]. The Ohio State University; 1965. [cited 2020 Jan 26]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu14865679584659.

Council of Science Editors:

Tomomatsu H. Structural studies on herapin. [Doctoral Dissertation]. The Ohio State University; 1965. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu14865679584659

University of Utah

9. Meola, Stacy D. Evaluation of antithrombogenic drug combination therapy utilizing an in vitro and ex vivo stent model;.

Degree: MS;, Pathology;, 2001, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1516/rec/467

► Anticoagulant and antiplatelet agents are currently being used clinically during episodes of unstable angina and non-Q-wave myocardial infarctions to stop thrombus formation to decrease morbidly… (more)

Subjects/Keywords: Heparin; Thrombus

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Meola, S. D. (2001). Evaluation of antithrombogenic drug combination therapy utilizing an in vitro and ex vivo stent model;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1516/rec/467

Chicago Manual of Style (16^th Edition):

Meola, Stacy D. “Evaluation of antithrombogenic drug combination therapy utilizing an in vitro and ex vivo stent model;.” 2001. Masters Thesis, University of Utah. Accessed January 26, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1516/rec/467.

MLA Handbook (7^th Edition):

Meola, Stacy D. “Evaluation of antithrombogenic drug combination therapy utilizing an in vitro and ex vivo stent model;.” 2001. Web. 26 Jan 2020.

Vancouver:

Meola SD. Evaluation of antithrombogenic drug combination therapy utilizing an in vitro and ex vivo stent model;. [Internet] [Masters thesis]. University of Utah; 2001. [cited 2020 Jan 26]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1516/rec/467.

Council of Science Editors:

Meola SD. Evaluation of antithrombogenic drug combination therapy utilizing an in vitro and ex vivo stent model;. [Masters Thesis]. University of Utah; 2001. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1516/rec/467

University of Hong Kong

10. Lau, Wing-yan. Cloning and characterization of heparanase 2 in rat brain.

Degree: M. Phil., 2016, University of Hong Kong

URL: http://hdl.handle.net/10722/225186

abstract

Biomedical Sciences

Master

Master of Philosophy

Subjects/Keywords: Heparin; Brain

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lau, W. (2016). Cloning and characterization of heparanase 2 in rat brain. (Masters Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/225186

Chicago Manual of Style (16^th Edition):

Lau, Wing-yan. “Cloning and characterization of heparanase 2 in rat brain.” 2016. Masters Thesis, University of Hong Kong. Accessed January 26, 2020. http://hdl.handle.net/10722/225186.

MLA Handbook (7^th Edition):

Lau, Wing-yan. “Cloning and characterization of heparanase 2 in rat brain.” 2016. Web. 26 Jan 2020.

Vancouver:

Lau W. Cloning and characterization of heparanase 2 in rat brain. [Internet] [Masters thesis]. University of Hong Kong; 2016. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/10722/225186.

Council of Science Editors:

Lau W. Cloning and characterization of heparanase 2 in rat brain. [Masters Thesis]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/225186

University of Hong Kong

11. 胡立基; Wu, Lap-kei. Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation.

Degree: PhD, 2015, University of Hong Kong

URL: Wu, L. [胡立基]. (2015). Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5481872 ; http://dx.doi.org/10.5353/th_b5481872 ; http://hdl.handle.net/10722/225837

► Deteriorating lung function is common in cigarette smoke related airway inflammation which is caused by unopposed neutrophil elastase (NE) activity that persists and disrupts the… (more)

▼ Deteriorating lung function is common in cigarette smoke related airway inflammation which is caused by unopposed neutrophil elastase (NE) activity that persists and disrupts the protease-anti-protease balance even after the primary cause has subsided. The focus of this work is on syndecan-1 (sdc-1) shedding in the affected airway. Our group has reported that in the sputum sol of patients with bronchiectasis, NE exists as supramolecular complexes with shed sdc-1, which were resistant to inhibition by i1-antitrypsin (11111). We hypothesized that similar mechanisms occur in the airway fluids of patients with chronic obstructive pulmonary disease and that the neutrophilic inflammation would be attenuated by (1) reduction of sdc-1 shedding and/or (2) displacement of NE from the supramolecular complexes. The first half of this work investigates the mechanism by which the heparan sulphate cleaving enzyme heparanase (HPSE) controls the shedding of sdc-1 from the airway epithelium. Western blot analysis of bronchial aspirates of patients demonstrated abundance of HPSE in the inflammatory airway fluid. With human bronchio-epithelial cells in air-liquid interface culture as model, HPSE activity robustly enhanced sdc-1 shedding as mediated by matrix metalloprotease-7 (MMP-7, matrilysin). This can be explained by the increased affinity of MMP-7 for the sdc-1 core following HPSE treatment of the heparan moiety. A similar increase in binding affinity between sdc-1 core and MMP-7 was also observed in bronchial aspirates of patients with airway inflammation. This points to HPSE inhibition as a strategy to reduce sdc-1 shedding. The second half of this work focuses on the use of an oligosaccharide heparin mimetic to treat cigarette smoke induced neutrophilic inflammation. In vitro tests showed that the heparin mimetic could displace NE from supramolecular complexes with sdc-1 in bronchial aspirates of patients with COPD. The displaced NE was then inhibited by endogenous wwwww. The heparin mimetic was also shown to be an inhibitor of HPSE activity and as-such was able to inhibit HPSE induced sdc-1 shedding from human bronchio-epithelial cells in vtiro. To test the efficacy of using this compound as a treatment for neutrophilic inflammation in the airway, rats were subjected to chronic cigarette smoke exposure and treated with a dry powder preparation of the heparin mimetic. Three consecutive doses of the heparin mimetic decreased both neutrophil count and NE activity in the lung compared to rats treated with carrier alone. Mean linear intercept of lung tissue sections from cigarette smoke exposed rats treated with the heparin mimetic were not different from the sham control group, demonstrating the effective protection of the lung against anatomical damage. Whole body plethysmography suggested improvement in lung function of treated animals compared to rats treated with carrier alone. This study provides insight on the mechanism by which HPSE modulates sdc-1 shedding and suggests a possible therapeutic for the treatment…

Subjects/Keywords: Bronchiectasis; Heparin; Leucocyte elastase

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

胡立基; Wu, L. (2015). Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. (Doctoral Dissertation). University of Hong Kong. Retrieved from Wu, L. [胡立基]. (2015). Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5481872 ; http://dx.doi.org/10.5353/th_b5481872 ; http://hdl.handle.net/10722/225837

Chicago Manual of Style (16^th Edition):

胡立基; Wu, Lap-kei. “Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation.” 2015. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020. Wu, L. [胡立基]. (2015). Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5481872 ; http://dx.doi.org/10.5353/th_b5481872 ; http://hdl.handle.net/10722/225837.

MLA Handbook (7^th Edition):

胡立基; Wu, Lap-kei. “Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation.” 2015. Web. 26 Jan 2020.

Vancouver:

胡立基; Wu L. Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. [Internet] [Doctoral dissertation]. University of Hong Kong; 2015. [cited 2020 Jan 26]. Available from: Wu, L. [胡立基]. (2015). Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5481872 ; http://dx.doi.org/10.5353/th_b5481872 ; http://hdl.handle.net/10722/225837.

Council of Science Editors:

胡立基; Wu L. Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. [Doctoral Dissertation]. University of Hong Kong; 2015. Available from: Wu, L. [胡立基]. (2015). Control of syndecan-1 shedding to limit smoking-related neutrophilic inflammation. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5481872 ; http://dx.doi.org/10.5353/th_b5481872 ; http://hdl.handle.net/10722/225837

Oregon State University

12. Bolsinger, Travis Samuel Sebastian. Human platelet adhesion to heparinized silica surfaces.

Degree: MS, Chemical Engineering, 2008, Oregon State University

URL: http://hdl.handle.net/1957/9292

► Heparin was modified and immobilized to surface-activated silica surfaces using two different reaction schemes. End-aminated heparin was reacted with 2-iminothiolane to produce free thiol groups… (more)

Subjects/Keywords: Heparin; Blood platelets – Aggregation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bolsinger, T. S. S. (2008). Human platelet adhesion to heparinized silica surfaces. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/9292

Chicago Manual of Style (16^th Edition):

Bolsinger, Travis Samuel Sebastian. “Human platelet adhesion to heparinized silica surfaces.” 2008. Masters Thesis, Oregon State University. Accessed January 26, 2020. http://hdl.handle.net/1957/9292.

MLA Handbook (7^th Edition):

Bolsinger, Travis Samuel Sebastian. “Human platelet adhesion to heparinized silica surfaces.” 2008. Web. 26 Jan 2020.

Vancouver:

Bolsinger TSS. Human platelet adhesion to heparinized silica surfaces. [Internet] [Masters thesis]. Oregon State University; 2008. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/1957/9292.

Council of Science Editors:

Bolsinger TSS. Human platelet adhesion to heparinized silica surfaces. [Masters Thesis]. Oregon State University; 2008. Available from: http://hdl.handle.net/1957/9292

McMaster University

13. Chung, Jason. A THROMBOELASTOGRAPHY STUDY ON THE EVALUATION OF CLOT FORMATION IN PLATELET-DEPLETED WHOLE BLOOD IN THE PRESENCE OF UNFRACTIONATED HEPARIN OR LOW-MOLECULAR WEIGHT HEPARIN.

Degree: MSc, 2015, McMaster University

URL: http://hdl.handle.net/11375/18361

►

The use of an appropriate anticoagulation regiment for the treatment of venous thromboembolism (VTE) in patients with concomitant thrombocytopenia is based on anecdotal evidence and… (more)

▼

The use of an appropriate anticoagulation regiment for the treatment of venous thromboembolism (VTE) in patients with concomitant thrombocytopenia is based on anecdotal evidence and the opinions of managing physicians. The current guidelines suggest that therapeutic levels of anticoagulants may be safely administered to patients who have a minimum platelet count of 50 x 109/L. However, it has recently been suggested that the minimal platelet threshold for safe anticoagulation treatment can be provided at a reduced platelet count of 30 x 109/L. Thus, in order evaluate these platelet threshold we used a thromboelastography (TEG) model to evaluate the clotting parameters of whole blood at predefined platelet counts in the presence of unfractionated heparin (UFH) and low-molecular weight heparin (LMWH). Due to the importance of red blood cells on hemostasis a whole blood TEG model was designed in order to mimic in vivo hemostasis. Clotting was initiated using different concentrations of tissue factor for each anticoagulant at therapeutic and prophylactic levels of UFH and LMWH at predefined platelet counts. In the presence of therapeutic concentrations of either UFH or LMWH, there were no significant differences in TEG parameters of whole blood clots between platelet counts of 30 x 109/L and 50 x 109/L when clotting was driven by the extrinsic pathway. At prophylactic levels of LMWH clot formation was less compromised. Furthermore, no significant difference was noted between platelet-depleted blood (PDB; <10 x 109/L) and 30 x 109/L with respect to r-time. This suggests LMWH at prophylactic levels has no significant bearing on clot formation at a lower platelet threshold versus therapeutic levels of LMWH. Overall, it shows that clot formation is similar for UFH and LMWH when platelet counts are reduced from 50 x 109/L to 30 x 109/L. This work provides insight on the potential for anticoagulation at a reduced platelet threshold in thrombocytopenic conditions.

Thesis

Master of Science (MSc)

Advisors/Committee Members: Chan, Howard, Health Sciences.

Subjects/Keywords: Thrombocytopenia; Thromboelastography; Heparin; Platelets

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chung, J. (2015). A THROMBOELASTOGRAPHY STUDY ON THE EVALUATION OF CLOT FORMATION IN PLATELET-DEPLETED WHOLE BLOOD IN THE PRESENCE OF UNFRACTIONATED HEPARIN OR LOW-MOLECULAR WEIGHT HEPARIN. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/18361

Chicago Manual of Style (16^th Edition):

Chung, Jason. “A THROMBOELASTOGRAPHY STUDY ON THE EVALUATION OF CLOT FORMATION IN PLATELET-DEPLETED WHOLE BLOOD IN THE PRESENCE OF UNFRACTIONATED HEPARIN OR LOW-MOLECULAR WEIGHT HEPARIN.” 2015. Masters Thesis, McMaster University. Accessed January 26, 2020. http://hdl.handle.net/11375/18361.

MLA Handbook (7^th Edition):

Chung, Jason. “A THROMBOELASTOGRAPHY STUDY ON THE EVALUATION OF CLOT FORMATION IN PLATELET-DEPLETED WHOLE BLOOD IN THE PRESENCE OF UNFRACTIONATED HEPARIN OR LOW-MOLECULAR WEIGHT HEPARIN.” 2015. Web. 26 Jan 2020.

Vancouver:

Chung J. A THROMBOELASTOGRAPHY STUDY ON THE EVALUATION OF CLOT FORMATION IN PLATELET-DEPLETED WHOLE BLOOD IN THE PRESENCE OF UNFRACTIONATED HEPARIN OR LOW-MOLECULAR WEIGHT HEPARIN. [Internet] [Masters thesis]. McMaster University; 2015. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/11375/18361.

Council of Science Editors:

Chung J. A THROMBOELASTOGRAPHY STUDY ON THE EVALUATION OF CLOT FORMATION IN PLATELET-DEPLETED WHOLE BLOOD IN THE PRESENCE OF UNFRACTIONATED HEPARIN OR LOW-MOLECULAR WEIGHT HEPARIN. [Masters Thesis]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/18361

McGill University

14. Glushka, John. The synthesis of disaccharides related to heparin.

Degree: PhD, Department of Chemistry., 1986, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile72844.pdf

Subjects/Keywords: Heparin; Disaccharides.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Glushka, J. (1986). The synthesis of disaccharides related to heparin. (Doctoral Dissertation). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile72844.pdf

Chicago Manual of Style (16^th Edition):

Glushka, John. “The synthesis of disaccharides related to heparin.” 1986. Doctoral Dissertation, McGill University. Accessed January 26, 2020. http://digitool.library.mcgill.ca/thesisfile72844.pdf.

MLA Handbook (7^th Edition):

Glushka, John. “The synthesis of disaccharides related to heparin.” 1986. Web. 26 Jan 2020.

Vancouver:

Glushka J. The synthesis of disaccharides related to heparin. [Internet] [Doctoral dissertation]. McGill University; 1986. [cited 2020 Jan 26]. Available from: http://digitool.library.mcgill.ca/thesisfile72844.pdf.

Council of Science Editors:

Glushka J. The synthesis of disaccharides related to heparin. [Doctoral Dissertation]. McGill University; 1986. Available from: http://digitool.library.mcgill.ca/thesisfile72844.pdf

University of Debrecen

15. Mező, Erika. Különböző útvonalak szulfonsav-tartalmú heparinoid triszacharid szintézisére .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Kémiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/107468

A diplomamunka szulfonsav-tartalmú heparinoid triszacharid szintézisét foglalja magába. Advisors/Committee Members: Lázár, László (advisor).

Subjects/Keywords: heparin; triszacharid

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mező, E. (2011). Különböző útvonalak szulfonsav-tartalmú heparinoid triszacharid szintézisére . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/107468

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mező, Erika. “Különböző útvonalak szulfonsav-tartalmú heparinoid triszacharid szintézisére .” 2011. Thesis, University of Debrecen. Accessed January 26, 2020. http://hdl.handle.net/2437/107468.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mező, Erika. “Különböző útvonalak szulfonsav-tartalmú heparinoid triszacharid szintézisére .” 2011. Web. 26 Jan 2020.

Vancouver:

Mező E. Különböző útvonalak szulfonsav-tartalmú heparinoid triszacharid szintézisére . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/2437/107468.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mező E. Különböző útvonalak szulfonsav-tartalmú heparinoid triszacharid szintézisére . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/107468

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

16. Takács, Róbert. A heparin indulálta thrombocytopénia laboratóriumi diagnosztikája .

Degree: DE – Általános Orvostudományi Kar, University of Debrecen

URL: http://hdl.handle.net/2437/201475

► A heparin terápia egyik súlyos következményekkel járó immunmediált mellékhatása a heparin-indukálta thrombocytopénia (HIT). Kialakulása mai napig magas morbiditással és moralitással jár. Klinikai jellegzetessége a heparin… (more)

▼ A heparin terápia egyik súlyos következményekkel járó immunmediált mellékhatása a heparin-indukálta thrombocytopénia (HIT). Kialakulása mai napig magas morbiditással és moralitással jár. Klinikai jellegzetessége a heparin terápia megkezdését követő 5. – 14. napon megfigyelhető thrombocytopénia, valamint prothromboticus állapot kialakulása. A tünetek hátterében a 4-es vérlemezke faktor (PF4) heparinhoz történő kapcsolódása következtében kialakuló PF4/heparin komplex ellen termelődő IgG típusú antitestek megjelenése áll. Az antitestek a thrombocyták felszínén található FcγIIa receptorokhoz (CD32) való kapcsolódás révén aktiválják a vérlemezkéket aminek hatására prothromboticus mikropartikulumok felszabadulása és a thrombocyták consumptiója következik be. Ugyanezen antitestek thrombocyta aktiváló hatásuk mellett az endothelsejteket valamint a monocytákat is aktiválva további prothromboticus folyamatokat indítanak be. Ezek a folyamtok közösen vezetnek a közepes fokú thrombocytopénia (60 – 90 G/L) és az emelkedett thrombosis hajlam kialakulásához. A HIT diagnózisa nem állítható fel kizárólag a klinikai tünetek alapján. Erre csak az IgG típusú HIT antitest laboratóriumi kimutatását követően kerülhet sor. Célunk a rendelkezésünkre álló nemzetközi szakirodalom felhasználásával bemutatni a HIT diagnosztizálásában szerepet játszó laboratóriumi módszereket, valamint a Debreceni Egyetem Orvos- és Egészségtudományi Centrum (DEOEC) Laboratóriumi Medicina Intézete (LMI) által alkalmazott HIT vizsgálati panelt. A HIT panel magába foglalja a thrombocyta szám meghatározását, partikulált gél immunoassayn (PaGIA) és ELISA teszt elvégzését illetve áramlási citométerrel elvégzett funkcionális vizsgálatot. A vizsgálati panel bevezetése óta a Laboratóriumi Medicina Intézetben 156 esetben indult kivizsgálás a HIT irányába, melyből 5 esetben igazolódott funkcionális teszttel is megerősített HIT. Mind a 156 beteg esetén elvégzésre került a PaGIA és ELISA teszt. Pozitív ELISA minták esetén áramlási citométerrel végzett funkcionális tesztet is végeztünk. Vizsgálati eredményeinket a nemzetközi szakirodalomban megtalálható adatokkal összevetve értékeltük. Advisors/Committee Members: Kerényi, Adrienne (advisor), Debreceni Egyetem::Orvos- és Egészségtudományi Centrum::Általános Orvostudományi Kar::Laboratóriumi Medicina Intézet (advisor).

Subjects/Keywords: heparin; thrombocytopénia

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Takács, R. (n.d.). A heparin indulálta thrombocytopénia laboratóriumi diagnosztikája . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/201475

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Takács, Róbert. “A heparin indulálta thrombocytopénia laboratóriumi diagnosztikája .” Thesis, University of Debrecen. Accessed January 26, 2020. http://hdl.handle.net/2437/201475.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Takács, Róbert. “A heparin indulálta thrombocytopénia laboratóriumi diagnosztikája .” Web. 26 Jan 2020.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Takács R. A heparin indulálta thrombocytopénia laboratóriumi diagnosztikája . [Internet] [Thesis]. University of Debrecen; [cited 2020 Jan 26]. Available from: http://hdl.handle.net/2437/201475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Takács R. A heparin indulálta thrombocytopénia laboratóriumi diagnosztikája . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/201475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Boston University

17. Teran, Madelane. Modulation of vascular endothelial growth factor receptor affinity by neuropilin-1 and heparan sulfate proteoglycans.

Degree: PhD, Biochemistry, 2016, Boston University

URL: http://hdl.handle.net/2144/14590

► Angiogenesis is a highly regulated process orchestrated by the vascular endothelial growth factor-A (VEGF-A) system of ligands and receptors. Heparin/heparan sulfate (HS) proteoglycans and neuropilin-1… (more)

▼ Angiogenesis is a highly regulated process orchestrated by the vascular endothelial growth factor-A (VEGF-A) system of ligands and receptors. Heparin/heparan sulfate (HS) proteoglycans and neuropilin-1 (NRP-1) have been identified as co-receptors for VEGF-A, yet the mechanisms of action have not been fully defined. In the present study, we characterized molecular interactions between receptors and co-receptors and the two major VEGF-A isoforms, using surface plasmon resonance (SPR) and in vitro binding assays. We found that VEGF dissociated 25-times faster from its major signaling receptor, VEGF receptor-2 (VEGFR-2) than from its “decoy” receptor, VEGF receptor-1 (VEGFR-1). We identified a potential mechanism for co-receptors to decrease the dissociation rate and prolong the signaling complex lifetime. Using a systematic approach, we obtained kinetic parameters for each individual interaction in an intercomparable way to measure the effect NRP-1 and HS have on complex stability. Additionally, we demonstrated that these binding events influence VEGF activity within endothelial cells. These parameters can be used in mathematical models to predict therapy outcomes in defined cellular contexts. Furthermore, we optimized a competition-based technique using SPR and structurally defined HS oligosaccharides and demonstrated that it can be used to rapidly measure affinities to HS-binding proteins. We used this method to define interactions and structural and length requirements for heparin/HS interactions with VEGFR-1, NRP-1, and VEGF165, the most relevant VEGF-A isoform, in complex with VEGFR-2 and NRP-1. We show that the structural requirements were distinct for each interaction. We further found that VEGF165, VEGFR-2 and monomeric NRP-1 bound weakly to heparin alone, yet binding to heparin increased synergistically when presented together. This enhanced binding correlated with alterations in VEGF signaling in endothelial cells. We found that soluble NRP-1 increased VEGF binding and activated phosphorylation of VEGFR-2 and Erk1/2 in endothelial cells, and that these effects required sulfated HS. These data suggest that the presence of HS/heparin and NRP-1 may dictate the specific receptor type activated by VEGF and ultimately determine the biological output. The ability of co-receptors to fine-tune VEGF responsiveness suggests the possibility that VEGF-mediated angiogenesis can be selectively stimulated or inhibited by targeting HS/heparin and NRP-1.

Subjects/Keywords: Biochemistry; Heparin; Proteoglycans; Endothelial; Vascular

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teran, M. (2016). Modulation of vascular endothelial growth factor receptor affinity by neuropilin-1 and heparan sulfate proteoglycans. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/14590

Chicago Manual of Style (16^th Edition):

Teran, Madelane. “Modulation of vascular endothelial growth factor receptor affinity by neuropilin-1 and heparan sulfate proteoglycans.” 2016. Doctoral Dissertation, Boston University. Accessed January 26, 2020. http://hdl.handle.net/2144/14590.

MLA Handbook (7^th Edition):

Teran, Madelane. “Modulation of vascular endothelial growth factor receptor affinity by neuropilin-1 and heparan sulfate proteoglycans.” 2016. Web. 26 Jan 2020.

Vancouver:

Teran M. Modulation of vascular endothelial growth factor receptor affinity by neuropilin-1 and heparan sulfate proteoglycans. [Internet] [Doctoral dissertation]. Boston University; 2016. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/2144/14590.

Council of Science Editors:

Teran M. Modulation of vascular endothelial growth factor receptor affinity by neuropilin-1 and heparan sulfate proteoglycans. [Doctoral Dissertation]. Boston University; 2016. Available from: http://hdl.handle.net/2144/14590

Georgia Tech

18. Lei, Jennifer. The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications.

Degree: PhD, Mechanical Engineering, 2016, Georgia Tech

URL: http://hdl.handle.net/1853/54949

► Mesenchymal stem cells are multipotent cells that have the ability to differentiate down multiple lineages as well as secrete trophic and anti-inflammatory factors. These qualities… (more)

▼ Mesenchymal stem cells are multipotent cells that have the ability to differentiate down multiple lineages as well as secrete trophic and anti-inflammatory factors. These qualities make MSCs a promising cell source for cell-based therapies to treat a variety of injuries and pathologies. Biomaterials are often used to control and direct stem cell behavior by engineering a desired environment around the cells. Recent research has focused on using the naturally derived sulfated glycosaminoglycan (GAG), heparin as a biomaterial due to its negative charge and ability to sequester and bind positively charged growth factors. Engineering a heparin coating that can mimic the native heparan sulfate proteoglycan structure found at cell surfaces can be used as a novel platform to present GAGs to cells to direct cell behavior. The overall goal of this dissertation was to develop GAG-based coatings on MSC spheroids in order to study the role of heparin and its derivatives on MSC culture applications. To investigate the role of heparin in coating form on MSC behavior, the ability of the coating to sequester positively charged growth factors was characterized. Given the role of sulfation in the negative charge density of heparin and growth factor interactions, a desulfated heparin coating was develop and used to examine how presentation of coatings with native and no sulfation levels could potentiate response to growth factors in the surrounding environment. Additionally, heparin and growth factor binding in coating presentation was explored to develop a novel platform to assemble MSC-based microtissues. Together these studies provided valuable insight into a novel approach to direct cell behavior by engineering a coating that harnesses heparin interactions with the surrounding environment. Advisors/Committee Members: Temenoff, Johnna S. (advisor), Botchwey, Edward A. (committee member), Guldberg, Robert E. (committee member), McDevitt, Todd C. (committee member), Murphy, William L. (committee member).

Subjects/Keywords: Heparin; Mesenchymal stem cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lei, J. (2016). The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/54949

Chicago Manual of Style (16^th Edition):

Lei, Jennifer. “The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications.” 2016. Doctoral Dissertation, Georgia Tech. Accessed January 26, 2020. http://hdl.handle.net/1853/54949.

MLA Handbook (7^th Edition):

Lei, Jennifer. “The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications.” 2016. Web. 26 Jan 2020.

Vancouver:

Lei J. The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications. [Internet] [Doctoral dissertation]. Georgia Tech; 2016. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/1853/54949.

Council of Science Editors:

Lei J. The development of glycosaminoglycan coatings for mesenchymal stem cell-based culture applications. [Doctoral Dissertation]. Georgia Tech; 2016. Available from: http://hdl.handle.net/1853/54949

University of Arkansas

19. Morris, Jacqueline Anastasia. Heparin-Peptide Interactions.

Degree: PhD, 2016, University of Arkansas

URL: https://scholarworks.uark.edu/etd/2645

► Heparin is a polydispersed sulfated molecule that is part of the family called glycosaminoglycans found in the extracellular matrix and cell surfaces. This molecule… (more)

▼ Heparin is a polydispersed sulfated molecule that is part of the family called glycosaminoglycans found in the extracellular matrix and cell surfaces. This molecule is extremely important for the activation of proteins and protein-receptor interactions that are responsible for downstream cell signaling pathways. Heparin has been isolated from porcine intestine and used as an anticoagulant for the prevention of embolisms, heart thrombosis, and clotting during heart surgeries. This so-called miracle drug was in use until 2008, when isolated batches were found to be contaminated with other glycosaminoglycans similar to heparin. From 2008, there has been a dire need for a more cost-effective purification of heparin in good yield, to remove the contaminants. Heparin has also been used for affinity chromatography to purify heparin-binding proteins. Recombinant protein purification is an important aspect of biotechnology, with a heavy emphasis on developing easy purification systems for large quantities of homogeneously pure therapeutic proteins at low costs. Several affinity tags have been employed to aid in the expression and purification of various proteins. There are, however, various disadvantages, such as interference with the target protein structure, unsuitable elution conditions, multiple-step purification procedures, and many others. In this context, a novel heparin-binding peptide (HB-peptide) was designed based on the heparin-binding region of acidic fibroblast growth factor (FGF-1). The HB-peptide has been shown to bind to heparin with better affinity than other glycosaminoglycans. A one-step Heparin-Sepharose-based purification procedure was developed for various recombinant proteins using mild elution conditions. Using proteolytic cleavage, the affinity tag was removed to obtain a folded protein. Polyclonal antibodies were also grown against the specific sequence of the HB-peptide, which then were used to successfully and specifically bind fusion proteins that only contained the HB-peptide even at the nanogram level. HB-peptide was then coupled onto a solid matrix to be used as an affinity column, in a similar fashion as heparin-Sepharose with HBPs. A mixture of glycosaminoglycans were separated from each other for the most part. In this context, our HB-peptide can be used as a means to separate glycosaminoglycans, yielding pure heparin for future medical use. Advisors/Committee Members: Thallapuranam Kumar, Roger Koeppe, Jingyi Chen.

Subjects/Keywords: Affinity Tag; Heparin; Heparin-binding Proteins; Analytical Chemistry; Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morris, J. A. (2016). Heparin-Peptide Interactions. (Doctoral Dissertation). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/2645

Chicago Manual of Style (16^th Edition):

Morris, Jacqueline Anastasia. “Heparin-Peptide Interactions.” 2016. Doctoral Dissertation, University of Arkansas. Accessed January 26, 2020. https://scholarworks.uark.edu/etd/2645.

MLA Handbook (7^th Edition):

Morris, Jacqueline Anastasia. “Heparin-Peptide Interactions.” 2016. Web. 26 Jan 2020.

Vancouver:

Morris JA. Heparin-Peptide Interactions. [Internet] [Doctoral dissertation]. University of Arkansas; 2016. [cited 2020 Jan 26]. Available from: https://scholarworks.uark.edu/etd/2645.

Council of Science Editors:

Morris JA. Heparin-Peptide Interactions. [Doctoral Dissertation]. University of Arkansas; 2016. Available from: https://scholarworks.uark.edu/etd/2645

20. Καστελλοριζιός, Μιχαήλ. Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό.

Degree: 2012, University of Patras

URL: http://hdl.handle.net/10889/5278

►

Τις τελευταίες δεκαετίες υπάρχει μια αυξανόμενη ζήτηση για βιοσυμβατά υλικά ικανά να χρησιμοποιηθούν σαν πρόσθετα στο ανθρώπινο σώμα, σαν βάσεις για ελεγχόμενη χορήγηση βιοδραστικών ενώσεων,… (more)

▼

Τις τελευταίες δεκαετίες υπάρχει μια αυξανόμενη ζήτηση για βιοσυμβατά υλικά ικανά να χρησιμοποιηθούν σαν πρόσθετα στο ανθρώπινο σώμα, σαν βάσεις για ελεγχόμενη χορήγηση βιοδραστικών ενώσεων, σαν εμφυτεύματα κ.α. χωρίς να προκαλούν ανοσοποιητική αντίδραση από τον αργανισμό. Μέχρι και σήμερα δεν έχει βρεθεί το υλικό που θα ξεγελάσει τους αμυντικούς μηχανισμούς του σώματος, με άλλα λόγια, να είναι αόρατο από το σώμα. Έχουν γίνει πολλές προσπάθειες και έχουν εφαρμοστεί διάφορες προσεγγίσεις. Τα τελευταία χρόνια όλο και περισσότερες ερευνητικές ομάδες επενδύουν σε υλικά τα οποία απελευθερώνουν ελεγχόμενα βιοδραστικές ουσίες που καταστέλουν την αντίδραση του οργανισμού. Μια τέτοια ουσία είναι η ηπαρίνη, ένα φυσικό αντιπηκτικό το οποίο μπορεί να χρησιμοποιηθεί για την αύξηση της αιμοσυμβατότητας αρτηριακών ενδοπροσθέσεων. Αναπτύξαμε μια μέθοδο για την ομοιοπολική, μη αναστρέψιμη πρόσδεση λιποσωμάτων σε μεταλλικές επιφάνειες, οι οποίες έχουν υποστεί επεξεργασία με τεχνολογία πλάσματος. Οι επιφάνειες φέρουν ελεύθερες καρβοξυλομάδες τις οποίες μπορούμε να εκμεταλλευτούμε για να συζεύξουμε πάνω τους λιποσώματα με αμινομάδες στην επιφάνειά τους (functionalized), μέσω δημιουργίας αμιδικού δεσμού. Οι μεταλλικές επιφάνειες που χρησιμοποιήσαμε ήταν SS-316 μεταλλικοί δίσκοι επεξεργασμένοι με τεχνολογία πλάσματος, και τις προμηθευτήκαμε από το Τμήμα Χημικών Μηχανικών του Πανεπιστημίου Πατρών και από το τμήμα Χημείας του Πανεπιστημίου του Μπάρι. Χρησιμοποιήσαμε μικρά μονοστιβαδιακά λιποσώματα (SUV) διαμέτρου της τάξης των 100 nm, με διάφορες λιπιδικές συστάσεις (PC, PC:Chol 4:1, DSPC:Chol 2:1). Χαρακτηρίσαμε τα functionalized λιποσώματά ως προς την ικανότητά τους να εγκλωβίζουν ηπαρίνη, το μέγεθος, τη διασπορά μεγέθους, το φορτίο της επιφάνειάς τους και τη σταθερότητά τους σε διάφορες συνθήκες. Τα αποτελέσματά μας δείχνουν ότι γενικά τα functionalized λιποσώματα συμπεριφέρονται σαν τυπικά λιποσώματα που φέρουν λιπίδιο με PEG ομάδα (pegylated λιποσώματα), ενώ για βέλτιστο εγκλωβισμό ηπαρίνης σημαντικό είναι το στάδιο λυοφιλοποίησης / επανασύστασης κατά την παρασκευή τους. Αποδείξαμε ότι τα functionalized λιποσώματα μπορούν να προσδεθούν σε μεταλλικές επιφάνειες (κατάλληλα επεξεργασμένες) διατηρώντας τη δομή τους και φέροντας ηπαρίνη ή άλλη υδρόφιλη ουσία στο εσωτερικό τους. Εφαρμόσαμε ένα απλό πρωτόκολλο δημιουργίας αμιδικού δεσμού, και το βελτιστοποιήσαμε ώστε να πάρουμε μέγιστη απόδοση στη σύνδεση των λιποσωμάτων στις επιφάνειες (58,4 ± 8.6 μg λιπιδίου ανά cm2 επιφάνειας).

Over the last couple of decades there has been an increasing need for more biocompatible, more “body friendly” materials that can be used in cases of subcutaneous, endoarterial or other type of implantations, as artificial body parts, drug releasing bases or biosensor implantations. The main desired quality of these materials is the lack to trigger the body’s defensive mechanisms and foreign body response reactions. Up to this date there has not been a material with the ability to camouflage itself and be invisible to the human body. Lately, scientists show…

Advisors/Committee Members: Αντιμησιάρη, Σοφία, Kastellorizios, Michael, Αντιμησιάρη, Σοφία, Κλεπετσάνης, Παύλος, Ματαράς, Δημήτριος.

Subjects/Keywords: Λιποσώματα; Ηπαρίνη; Επαναστένωση; 571.655; Liposomes; Heparin; Restenosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Καστελλοριζιός, �. (2012). Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/5278

Chicago Manual of Style (16^th Edition):

Καστελλοριζιός, Μιχαήλ. “Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό.” 2012. Masters Thesis, University of Patras. Accessed January 26, 2020. http://hdl.handle.net/10889/5278.

MLA Handbook (7^th Edition):

Καστελλοριζιός, Μιχαήλ. “Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό.” 2012. Web. 26 Jan 2020.

Vancouver:

Καστελλοριζιός �. Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό. [Internet] [Masters thesis]. University of Patras; 2012. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/10889/5278.

Council of Science Editors:

Καστελλοριζιός �. Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό. [Masters Thesis]. University of Patras; 2012. Available from: http://hdl.handle.net/10889/5278

University of Hong Kong

21. 楊文雅.; Yeung, Man-nga. Functional roles of heparanase in endochondral ossification.

Degree: PhD, 2009, University of Hong Kong

URL: Yeung, M. [楊文雅]. (2009). Functional roles of heparanase in endochondral ossification. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4322402 ; http://dx.doi.org/10.5353/th_b4322402 ; http://hdl.handle.net/10722/56792

published_or_final_version

Biochemistry

Doctoral

Doctor of Philosophy

Advisors/Committee Members: Shum, DKY.

Subjects/Keywords: Endochondral ossification.; Heparin.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

楊文雅.; Yeung, M. (2009). Functional roles of heparanase in endochondral ossification. (Doctoral Dissertation). University of Hong Kong. Retrieved from Yeung, M. [楊文雅]. (2009). Functional roles of heparanase in endochondral ossification. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4322402 ; http://dx.doi.org/10.5353/th_b4322402 ; http://hdl.handle.net/10722/56792

Chicago Manual of Style (16^th Edition):

楊文雅.; Yeung, Man-nga. “Functional roles of heparanase in endochondral ossification.” 2009. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020. Yeung, M. [楊文雅]. (2009). Functional roles of heparanase in endochondral ossification. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4322402 ; http://dx.doi.org/10.5353/th_b4322402 ; http://hdl.handle.net/10722/56792.

MLA Handbook (7^th Edition):

楊文雅.; Yeung, Man-nga. “Functional roles of heparanase in endochondral ossification.” 2009. Web. 26 Jan 2020.

Vancouver:

楊文雅.; Yeung M. Functional roles of heparanase in endochondral ossification. [Internet] [Doctoral dissertation]. University of Hong Kong; 2009. [cited 2020 Jan 26]. Available from: Yeung, M. [楊文雅]. (2009). Functional roles of heparanase in endochondral ossification. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4322402 ; http://dx.doi.org/10.5353/th_b4322402 ; http://hdl.handle.net/10722/56792.

Council of Science Editors:

楊文雅.; Yeung M. Functional roles of heparanase in endochondral ossification. [Doctoral Dissertation]. University of Hong Kong; 2009. Available from: Yeung, M. [楊文雅]. (2009). Functional roles of heparanase in endochondral ossification. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4322402 ; http://dx.doi.org/10.5353/th_b4322402 ; http://hdl.handle.net/10722/56792

University of Saskatchewan

22. Duckworth, Shannon Elissa. Effect of oral heparin on homocysteine induced changes in hemodynamic parameters and oxidative stress.

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-01132011-124515

► Several studies have found a positive correlation between hypertension and hyperhomocysteinemia. Increasing evidence implicates oxidative stress as one of the initiating events closely linked to… (more)

▼ Several studies have found a positive correlation between hypertension and hyperhomocysteinemia. Increasing evidence implicates oxidative stress as one of the initiating events closely linked to the homocysteine’s ability to damage endothelium, subsequently causing vascular dysfunction. We previously found that heparin protects cultured endothelial cells from free radical injury and oral heparin at 1 mg/kg/48h prevents venous thrombosis in a rat model in vivo. Our objective was to study the protective effects of oral heparin in a rat model with elevated plasma homocysteine (Hcy) concentrations, and begin to elucidate whether the pathophysiological effects of Hcy are mediated through an oxidative mechanism causing endothelial dysfunction. Elevated plasma Hcy levels were induced by feeding male Wistar Kyoto rats a diet containing an additional 1.7% methionine for 8 weeks. Groups included rats fed additional methionine, methionine plus oral heparin (1 mg/kg/48h by gastric feeding tube), and age-matched controls fed normal rat chow. At the end of 8 weeks of treatment, rats were anesthetized using 1.5% isoflurane in 100% oxygen. Hemodynamics parameters were assessed by inserting a Millar Mikro Tip pressure transducer into the left ventricular chamber and the thoracic aorta. Fasting plasma total Hcy levels were measured using a Hcy immunoassay kit with an Abbott IMx instrument. Malondialdehyde (MDA) concentrations, a lipid peroxidation product and marker for oxidative stress, was measured by a spectrophotometric method in serum and tissue samples. Glutathione (GSH) concentrations, an important antioxidant for low-level oxidative stress was measured by HPLC in plasma and tissues samples. Lastly, tissue samples from each experimental group were stained with the TUNEL method to assess their respective percentage of apoptotic endothelial cells. Results were expressed as mean ± S.E. Unpaired Student’s two-tailed t-test was employed to assess the difference between groups with p < 0.05 considered significant. Plasma Hcy was significantly elevated after 8 weeks in the methionine (7.17 ± 0.46 umol/L) and methionine plus heparin treated rats (7.02 ± 0.40 umol/L) compared to control (5.46 ± 0.36 umol/L). All measures of arterial pressure, systolic (SP) and diastolic pressure (DP) and mean arterial pressure (MAP), were significantly elevated in rats fed the methionine diet without heparin (119.9 ± 3.9 mmHg; 90.3 ± 3.5 mmHg; 97.7 ± 2.9 mmHg, respectively) compared to controls (107.8 ± 2.5 mmHg; 79.2 ± 2.1 mmHg; 88.8 ± 2.2 mmHg, respectively) but not compared to heparin (114.7 ± 3.3 mmHg; 83.4 ± 2.4 mmHg; 93.8 ± 2.7 mmHg, respectively). Left ventricular end diastolic pressure (LVEDP) was significantly elevated with the methionine diet without heparin (14.2 ± 2.5 mmHg) but not with heparin treatment (8.4 ± 1.9 mmHg) versus controls (7.1 ± 1.1 mmHg). Also, left ventricular systolic pressure (LVSP) was significantly elevated in the methionine fed rats after 8 weeks (122.6 ± 3.2 mmHg) compared to controls (112.3. ± 2.9… Advisors/Committee Members: Hiebert, Linda, Lee, Paul, Prasad, Kailash, Desautels, Michel, Fisher, Thomas, Bharadwaj, Lalita.

Subjects/Keywords: heparin; hypertension; homocysteine; glutathione; oxidative stress; apoptosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Duckworth, S. E. (2010). Effect of oral heparin on homocysteine induced changes in hemodynamic parameters and oxidative stress. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-01132011-124515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Duckworth, Shannon Elissa. “Effect of oral heparin on homocysteine induced changes in hemodynamic parameters and oxidative stress.” 2010. Thesis, University of Saskatchewan. Accessed January 26, 2020. http://hdl.handle.net/10388/etd-01132011-124515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Duckworth, Shannon Elissa. “Effect of oral heparin on homocysteine induced changes in hemodynamic parameters and oxidative stress.” 2010. Web. 26 Jan 2020.

Vancouver:

Duckworth SE. Effect of oral heparin on homocysteine induced changes in hemodynamic parameters and oxidative stress. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/10388/etd-01132011-124515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Duckworth SE. Effect of oral heparin on homocysteine induced changes in hemodynamic parameters and oxidative stress. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-01132011-124515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Roam, Jacob. Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration.

Degree: PhD, Biomedical Engineering, 2015, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/eng_etds/116

► Many biological processes depend on concentration gradients in signaling molecules. Thus, introduction of spatial patterning of proteins, while retaining activity and releasability, will be… (more)

▼ Many biological processes depend on concentration gradients in signaling molecules. Thus, introduction of spatial patterning of proteins, while retaining activity and releasability, will be critical for the field of regenerative medicine. In particular, the area of nerve regeneration is in need of innovations to improve outcomes. Only about 25% of surgical patients with peripheral nerve damage (~200,000 surgical interventions performed each year) regain full motor function with less than 3% regaining sensation. The use of nerve guidance conduits (NGC’s) which are filled with biomimetic scaffolds is one treatment being explored. These scaffolds, however, lack the spatial patterning of proteins found in native tissue. Glial-cell line derived neurotrophic factor (GDNF), a potent stimulator of axon regeneration, is one such protein that, if contained within the scaffold and conformed to a particular concentration profile, could greatly enhance neural regeneration. The object of this work is to utilize poly(ethylene glycol) (PEG) microspheres to accomplish this spatial patterning of GDNF and apply it to NGC’s. First, an approach utilizing the controllability of the PEG microsphere’s density (buoyancy) was explored. By creating the microspheres under varying conditions, incubation time and temperature, the cross-linking and, thus, the swelling rate of the microspheres could be controlled. This created microspheres of different densities that, upon centrifugation, would orient themselves within a scaffold, creating a gradient in the different microsphere types. GDNF loaded into a batch of microspheres would thusly be oriented within the scaffold along with that particular microsphere batch. Through this, gradients in GDNF were created. Heparin was also added to the microspheres to allow for reversible binding of GDNF. Next, gradients in reversibly bound GDNF were formed through sequential centrifugation of microsphere batches. For instance, a layer of GDNF loaded microspheres were formed into a scaffold followed by a layer of microspheres without GDNF on top of them. This created an initial step gradient in GDNF that, given time to release, would form a linear concentration gradient. Gradients formed by this method were visualized by fluorescent confocal microscopy and compared to Fickian models. Some conditions yielded profiles more linear than the model predictions, which persisted for over a week. Lastly, the sequential gradient formation was modified and applied to NGC’s. Before the scaffolds were ready for in vivo implantation, functionalities such as cell initiated degradability, cell adhesion, and inter-microsphere cross-linking were added. A plasmin degradable peptide sequence (GCGGVRNGGK) was incorporated into the microspheres. CLICK agents, laminin, and heparin (via a new binding chemistry) were attached to the microspheres to add inter-microsphere cross-linking, add cell adhesion, and heparin binding functionalities, respectively. GDNF gradient formation and activity retention were confirmed… Advisors/Committee Members: Donald L Elbert, Shelly Sakiyama-Elbert, Matthew Wood, Dennis Barbour, Jin-Yu Shao.

Subjects/Keywords: degradable; GDNF; gradient; heparin; NGC; PEG; Engineering

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roam, J. (2015). Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/116

Chicago Manual of Style (16^th Edition):

Roam, Jacob. “Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration.” 2015. Doctoral Dissertation, Washington University in St. Louis. Accessed January 26, 2020. https://openscholarship.wustl.edu/eng_etds/116.

MLA Handbook (7^th Edition):

Roam, Jacob. “Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration.” 2015. Web. 26 Jan 2020.

Vancouver:

Roam J. Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2015. [cited 2020 Jan 26]. Available from: https://openscholarship.wustl.edu/eng_etds/116.

Council of Science Editors:

Roam J. Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration. [Doctoral Dissertation]. Washington University in St. Louis; 2015. Available from: https://openscholarship.wustl.edu/eng_etds/116

NSYSU

24. Yin, Yao-De. The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin.

Degree: Master, Institute of Biomedical Sciences, 2018, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541

► Aptamers are single-stranded DNA or RNA fragments that bind to targeted molecules via folding into specific structure. Thus, aptamers can be employed as aptasensors for… (more)

▼ Aptamers are single-stranded DNA or RNA fragments that bind to targeted molecules via folding into specific structure. Thus, aptamers can be employed as aptasensors for detecting targeted molecules. The aim of this study was to investigate the utility of DNA aptamers against Bungarus multicinctus (Taiwan banded krait) presynaptic neurotoxin Î²-bungarotoxin (Î²-Bgt) as biosensors. Four Î²-Bgt aptamers, Bgt1, Bgt2, Bgt3, and Bgt4 were synthesized according to previously published results. Electrophoretic mobility shift assay showed that Î²-Bgt reduced the electrophoretic mobility of the aptamers. Moreover, Î²-Bgt reduced fluorescence intensity of the aptamers labeled with 5'-end carboxyfluorescein (FAM) and 3'-end 4-([4-(dimethylamino)-phenyl]azo)-benzoic acid (Dabcyl). These results showed that the folded structure of aptamers changed upon binding with Î²-Bgt. Measurement of the changes in FAM fluorescence revealed that Î²-Bgt had the highest binding affinity for Bgt1 among the four aptamers. In contrast to palmatine and berberine, coralyne drastically reduced FAM fluorescence intensity of 5'-FAM-Bgt1 and 5'-FAM-Bgt1-3'-Dabcyl, suggesting the selectivity of the sensors for detecting coralyne. The limit of detection (LOD) of coralyne determined by 5'-FAM-Bgt1 and 5'-FAM-Bgt1-3'-Dabcyl were 0.034 Î¼M and 0.021 Î¼M, respectively. Furthermore, coralyne-5'-FAM-Bgt1 and coralyne-5'-FAM-Bgt1-3'-Dabcyl complexes were employed as turn-on fluorescent sensors for detecting Î²-Bgt and heparin. Î²-Bgt and heparin notably restored FAM fluorescence intensity of coralyne-5'-FAM-Bgt1 and coralyne-5'-FAM-Bgt1-3'-Dabcyl complexes. The LOD of Î²-Bgt measured by coralyne-5'-FAM-Bgt1 and coralyne-5'-FAM-Bgt1-3'-Dabcyl complexes were 3.57 nM and 2.74 nM, respectively; the LOD of heparin were 0.012 Î¼g/ml and 0.004 Î¼g/ml, respectively; and the LOD for heparin-spiked serum were 0.027 Î¼g/ml and 0.009 Î¼g/ml, respectively. The sensor system constructed by coralyne and Bgt1 showed a high selectivity for heparin detection than for other glycosaminoglycans including chondroitin sulfate and hyaluronic acid. Although the sensor system effectively differentiated Î²-Bgt from other purified snake venom proteins, it could not specifically detect Taiwan banded krait crude venom. Taken together, our data indicate that aptamer against Î²-Bgt can be used for constructing biosensors for detecting coralyne and heparin. However, the sensor system is unable to identify the crude venoms of different snake species via Î²-Bgt detection. Advisors/Committee Members: Bin-Nan Wu (chair), Long-Sen Chang (committee member), Ying-Jung Chen (chair).

Subjects/Keywords: DNA aptamer; Sensors; Heparin; Coralyne; Î²-Bungarotoxin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yin, Y. (2018). The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yin, Yao-De. “The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin.” 2018. Thesis, NSYSU. Accessed January 26, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yin, Yao-De. “The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin.” 2018. Web. 26 Jan 2020.

Vancouver:

Yin Y. The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin. [Internet] [Thesis]. NSYSU; 2018. [cited 2020 Jan 26]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yin Y. The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

25. Joshi, Omkar. Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects.

Degree: MS, Chemical Engineering, 2004, Oregon State University

URL: http://hdl.handle.net/1957/22631

► Adipic dihydrazide-modified heparin was prepared and subsequently immobilized on surface-activated silica wafers. X-ray photoelectron spectroscopy (XPS) was used to quantify the elemental composition of the… (more)

Subjects/Keywords: Heparin – Physiological effect

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Joshi, O. (2004). Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/22631

Chicago Manual of Style (16^th Edition):

Joshi, Omkar. “Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects.” 2004. Masters Thesis, Oregon State University. Accessed January 26, 2020. http://hdl.handle.net/1957/22631.

MLA Handbook (7^th Edition):

Joshi, Omkar. “Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects.” 2004. Web. 26 Jan 2020.

Vancouver:

Joshi O. Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/1957/22631.

Council of Science Editors:

Joshi O. Fibrinogen adsorption at heparinized silica interfaces : history dependence and protein concentration effects. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/22631

Oregon State University

26. Lee, Hyo Jin. Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects.

Degree: MS, Chemical Engineering, 2004, Oregon State University

URL: http://hdl.handle.net/1957/22596

► Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages… (more)

Subjects/Keywords: Heparin – Physiological effect

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, H. J. (2004). Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/22596

Chicago Manual of Style (16^th Edition):

Lee, Hyo Jin. “Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects.” 2004. Masters Thesis, Oregon State University. Accessed January 26, 2020. http://hdl.handle.net/1957/22596.

MLA Handbook (7^th Edition):

Lee, Hyo Jin. “Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects.” 2004. Web. 26 Jan 2020.

Vancouver:

Lee HJ. Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2020 Jan 26]. Available from: http://hdl.handle.net/1957/22596.

Council of Science Editors:

Lee HJ. Fibrinogen adsorption at heparinized silica interfaces : history dependence and adsorption time effects. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/22596

University of Debrecen

27. Varga, Eszter. Véralvadásgátló hatású pentaszacharid-szulfonsav származék szintézise .

Degree: DE – Gyógyszerésztudományi Kar, University of Debrecen

URL: http://hdl.handle.net/2437/227916

► Kutatócsoportunkban új típusú antikoagulánsok kifejlesztését tűztük ki célul, melyekben a heparin-analóg pentaszacharidok farmakofór szulfátészter-csoportjait bioizoszter metánszulfonsav-csoporttal helyettesítjük. Ezáltal a biológiai környezetben való stabilitás növekedését vártuk.… (more)

Subjects/Keywords: heparin; pentaszacharid; antikoaguláns

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Varga, E. (n.d.). Véralvadásgátló hatású pentaszacharid-szulfonsav származék szintézise . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/227916

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Varga, Eszter. “Véralvadásgátló hatású pentaszacharid-szulfonsav származék szintézise .” Thesis, University of Debrecen. Accessed January 26, 2020. http://hdl.handle.net/2437/227916.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Varga, Eszter. “Véralvadásgátló hatású pentaszacharid-szulfonsav származék szintézise .” Web. 26 Jan 2020.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Varga E. Véralvadásgátló hatású pentaszacharid-szulfonsav származék szintézise . [Internet] [Thesis]. University of Debrecen; [cited 2020 Jan 26]. Available from: http://hdl.handle.net/2437/227916.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Varga E. Véralvadásgátló hatású pentaszacharid-szulfonsav származék szintézise . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/227916

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Debrecen

28. Shode, Deborah Temitope. Rare aspects of heparin-induced thrombocytopenia .

Degree: DE – Általános Orvostudományi Kar, University of Debrecen

URL: http://hdl.handle.net/2437/229981

Heparins are widely used anticoagulants but they are associated with limb-and life-threatening complications. This thesis discusses the rare but devastating pro-thrombotic complications of anticoagulant therapy which clinicans should be aware of. Advisors/Committee Members: Oláh, Zsolt (advisor), Debreceni Egyetem::Általános Orvostudományi Kar::Belgyógyászati Intézet (advisor).

Subjects/Keywords: Heparin; Induced; Thrombocytopenia

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shode, D. T. (n.d.). Rare aspects of heparin-induced thrombocytopenia . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/229981

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shode, Deborah Temitope. “Rare aspects of heparin-induced thrombocytopenia .” Thesis, University of Debrecen. Accessed January 26, 2020. http://hdl.handle.net/2437/229981.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shode, Deborah Temitope. “Rare aspects of heparin-induced thrombocytopenia .” Web. 26 Jan 2020.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Shode DT. Rare aspects of heparin-induced thrombocytopenia . [Internet] [Thesis]. University of Debrecen; [cited 2020 Jan 26]. Available from: http://hdl.handle.net/2437/229981.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Shode DT. Rare aspects of heparin-induced thrombocytopenia . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/229981

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of New South Wales

29. Young, Cara. Fabrication and characterisation of poly(vinyl alcohol) and heparin biosynthetic hydrogel microspheres for cell microencapsulation.

Degree: Graduate School of Biomedical Engineering, 2012, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/51980 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10650/SOURCE01?view=true

► Microencapsulation provides immunoisolation for encapsulated cells and allows them to be transplanted to replace damaged cell function and treat diseases such as diabetes. The use… (more)

▼ Microencapsulation provides immunoisolation for encapsulated cells and allows them to be transplanted to replace damaged cell function and treat diseases such as diabetes. The use of biosynthetic hydrogels made from poly (vinyl alcohol) (PVA) covalently bound with heparin as microencapsulation materials offer significant benefits over conventional alginate based systems due to the stability and reproducibility provided by the synthetic component and the bioactivity provided by the biological component. Despite advantages shown in bulk encapsulation, biosynthetic gels have not yet been applied to microencapsulation, due to lack of suitable methods for microsphere fabrication from biological and synthetic polymers in the presence of cells.Microsphere generation methods were investigated for use with photopolymerisation. A submerged electrospray droplet generation method was developed which produced spherical particles with controllable mean size, but a wide size distribution. In an effort to produce more uniform spheres, a microfluidic method was developed which produced smooth, spherical microspheres, with a monodisperse size distribution. The glycosaminoglycan heparin was then incorporated into these spheres. There was no effect on size, morphology or mechanics with heparin incorporation, and heparin remained covalently bound and biologically active. Heparin was seen to phase separate, particularly in spheres produced via microfluidics.Cells were then encapsulated in PVA/heparin gels both in bulk form and in microspheres. L929 fibroblasts, as a model cell line, survived encapsulation and remained viable up to 28 days after encapsulation in both PVA and PVA/heparin gels and microspheres made from both methods. However viability was significantly higher in PVA/heparin gels after 14 days in culture, and the microfluidic method resulted in slightly higher viability over time. The therapeutic insulin producing MIN6 beta cells survived encapsulation in bulk gels, and were more metabolically active, and released more insulin over time in PVA/heparin gels, as compared to PVA alone.This work has demonstrated two novel methods for the fabrication of biosynthetic microspheres and shown that the incorporation of heparin has minimal effect on sphere formation and significantly improves encapsulated cell viability and function over time. This concept may be applied in many microencapsulation applications to improve outcomes of cell transplantation therapies.

Subjects/Keywords: Hydrogel; Microencapsulation; Poly (vinyl alcohol); Heparin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Young, C. (2012). Fabrication and characterisation of poly(vinyl alcohol) and heparin biosynthetic hydrogel microspheres for cell microencapsulation. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/51980 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10650/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Young, Cara. “Fabrication and characterisation of poly(vinyl alcohol) and heparin biosynthetic hydrogel microspheres for cell microencapsulation.” 2012. Doctoral Dissertation, University of New South Wales. Accessed January 26, 2020. http://handle.unsw.edu.au/1959.4/51980 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10650/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Young, Cara. “Fabrication and characterisation of poly(vinyl alcohol) and heparin biosynthetic hydrogel microspheres for cell microencapsulation.” 2012. Web. 26 Jan 2020.

Vancouver:

Young C. Fabrication and characterisation of poly(vinyl alcohol) and heparin biosynthetic hydrogel microspheres for cell microencapsulation. [Internet] [Doctoral dissertation]. University of New South Wales; 2012. [cited 2020 Jan 26]. Available from: http://handle.unsw.edu.au/1959.4/51980 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10650/SOURCE01?view=true.

Council of Science Editors:

Young C. Fabrication and characterisation of poly(vinyl alcohol) and heparin biosynthetic hydrogel microspheres for cell microencapsulation. [Doctoral Dissertation]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/51980 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10650/SOURCE01?view=true

Princeton University

30. Raitman Khutorskoy, Irene. HEPARIN-FIBRONECTIN INTERACTIONS IN THE DEVELOPMENT OF EXTRACELLULAR MATRIX INSOLUBILITY .

Degree: PhD, 2018, Princeton University

URL: http://arks.princeton.edu/ark:/88435/dsp01df65vb53b

► The extracellular matrix (ECM) is composed of a network of fibrous proteins and proteoglycans that together provide stability, support, and regulate cell behavior and organization.… (more)

▼ The extracellular matrix (ECM) is composed of a network of fibrous proteins and proteoglycans that together provide stability, support, and regulate cell behavior and organization. Fibronectin (FN) is one of these fibrous proteins, and cell-mediated interactions between FN dimers form fibrils that, over time, become insoluble. This insoluble FN matrix serves an important role as a foundational matrix for other ECM proteins to build upon. The insoluble nature of FN fibrils provides the ECM with its structure and rigidity, and is important for normal developmental processes. A dysregulation in the assembly or accumulation of these fibrils can lead to various developmental and progressive diseases. Understanding how FN fibrils become stable and thus insoluble can help in better understanding such complications. We have developed a procedure to isolate insoluble fibril fragments from a decellularized matrix using proteolysis, deoxycholate (DOC) detergent extraction, and gel electrophoresis. Analyses of these DOC-insoluble fragments using domain-specific antibodies and mass spectrometry have identified three regions that are resistant to proteolysis. Two of these regions contain known heparin/heparan sulfate (HS) binding sites in FN. To determine whether heparin-FN interactions facilitate fibril assembly, a HS-deficient CHO cell line was used for matrix assembly experiments. In the absence of heparin, these cells assembled a limited number of FN fibrils. In contrast, the addition of heparin significantly increased the amount of FN matrix. Time course experiments indicated that heparin acts at an early step in the assembly process, and is required to be present continuously for maximal matrix stimulation. Sulfated glycosaminoglycan (GAG) mimetics similarly increased FN assembly and demonstrated a dependence on GAG sulfation. The length of the heparin chains also played a role in assembly. Chains of sufficient length to bind to two FN molecules gave maximal stimulation of assembly, whereas shorter heparin had less of an effect. Heparin was also able to bind to multiple heparin-binding domain FN fragments, as revealed by size exclusion chromatography analyses. A model is proposed in which heparin/HS binding at the heparin-binding domains connects multiple FNs together and promotes conformational changes involved in forming insoluble protein interactions. Advisors/Committee Members: Schwarzbauer, Jean E (advisor).

Subjects/Keywords: extracellular matrix; fibronectin; glycosaminoglycans; heparin; matrix assembly

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Raitman Khutorskoy, I. (2018). HEPARIN-FIBRONECTIN INTERACTIONS IN THE DEVELOPMENT OF EXTRACELLULAR MATRIX INSOLUBILITY . (Doctoral Dissertation). Princeton University. Retrieved from http://arks.princeton.edu/ark:/88435/dsp01df65vb53b

Chicago Manual of Style (16^th Edition):

Raitman Khutorskoy, Irene. “HEPARIN-FIBRONECTIN INTERACTIONS IN THE DEVELOPMENT OF EXTRACELLULAR MATRIX INSOLUBILITY .” 2018. Doctoral Dissertation, Princeton University. Accessed January 26, 2020. http://arks.princeton.edu/ark:/88435/dsp01df65vb53b.

MLA Handbook (7^th Edition):

Raitman Khutorskoy, Irene. “HEPARIN-FIBRONECTIN INTERACTIONS IN THE DEVELOPMENT OF EXTRACELLULAR MATRIX INSOLUBILITY .” 2018. Web. 26 Jan 2020.

Vancouver:

Raitman Khutorskoy I. HEPARIN-FIBRONECTIN INTERACTIONS IN THE DEVELOPMENT OF EXTRACELLULAR MATRIX INSOLUBILITY . [Internet] [Doctoral dissertation]. Princeton University; 2018. [cited 2020 Jan 26]. Available from: http://arks.princeton.edu/ark:/88435/dsp01df65vb53b.

Council of Science Editors:

Raitman Khutorskoy I. HEPARIN-FIBRONECTIN INTERACTIONS IN THE DEVELOPMENT OF EXTRACELLULAR MATRIX INSOLUBILITY . [Doctoral Dissertation]. Princeton University; 2018. Available from: http://arks.princeton.edu/ark:/88435/dsp01df65vb53b

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations