subject:(HepG2)

University of Debrecen

1. Rindt, Péter. Különböző emlős sejtvonalak transzfekciójának optimalizálása .

Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2014, University of Debrecen

URL: http://hdl.handle.net/2437/191340

A szakdolgozatom során HepG2 és MOVAS emlős sejtvonalak további transzfekciós vizsgálatához PEI reagenssel sikeresen optimalizáltuk a körülményeket. Advisors/Committee Members: Szántó, Magdolna (advisor).

Subjects/Keywords: transzfekció; PEI; HepG2; MOVAS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rindt, P. (2014). Különböző emlős sejtvonalak transzfekciójának optimalizálása . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/191340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rindt, Péter. “Különböző emlős sejtvonalak transzfekciójának optimalizálása .” 2014. Thesis, University of Debrecen. Accessed January 22, 2021. http://hdl.handle.net/2437/191340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rindt, Péter. “Különböző emlős sejtvonalak transzfekciójának optimalizálása .” 2014. Web. 22 Jan 2021.

Vancouver:

Rindt P. Különböző emlős sejtvonalak transzfekciójának optimalizálása . [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2437/191340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rindt P. Különböző emlős sejtvonalak transzfekciójának optimalizálása . [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/191340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Valencia

2. Tatay Dualde, Elena. Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares .

Degree: 2017, Universitat de Valencia

URL: http://hdl.handle.net/10550/61140

► La zearalenona (ZEA) y sus metabolitos α-zearalenol (α-ZOL) y β-zearalenol (β-ZOL), son metabolitos secundarios de hongos del genero Fusarium. La ZEA se absorbe y se… (more)

▼ La zearalenona (ZEA) y sus metabolitos α-zearalenol (α-ZOL) y β-zearalenol (β-ZOL), son metabolitos secundarios de hongos del genero Fusarium. La ZEA se absorbe y se metaboliza a α-ZOL y β-ZOL. Tanto la ZEA como sus metabolitos producen sus efectos tóxicos por la interacción con los receptores endocrinos aunque no es el único mecanismo por el cual pueden producir toxicidad. Se sabe que, además de disruptores endocrinos la ZEA y sus metabolitos son inmunotóxicos, hepatotóxicos y hematotóxicos causando alteraciones en el sistema reproductivo. La citotoxicidad de la ZEA y sus metabolitos ha sido determinada por distintos tiempos de exposición en diferentes células de mamíferos obteniéndose diferentes valores de IC50. Se investigó la conversión de ZEA y sus metabolitos en estos u otros derivados de ZEA por células CHO-K1 y HepG2 observando diferentes derivados de la ZEA y sus metabolitos. La exposición a estas micotoxinas causa estrés oxidativo observándose un aumento de las especies reactivas de oxigeno (ROS) que es proporcional al tiempo de exposición y la concentración de las micotoxinas. Se ha visto que este aumento de ROS conduce a una alteración de las vías metabólicas, disminución de los antioxidantes celulares y provoca que se oxiden estructuras macromoleculares, entre las que se encuentran las proteínas y lípidos de las membranas celulares y el ADN. Se evaluó la actividad estrogénica de la ZEA y sus metabolitos usando células de cáncer de mama humano (MCF-7) y se observó un aumento de esta actividad siendo el α-ZOL el que producía un mayor aumento de la actividad estrogénica. Se determinó si la ZEA y sus metabolitos activan los mecanismos de defensas antioxidantes en las células CHO-K1 y HepG2. Para ello, se determinó la actividad del glutatión (GSH) y de las enzimas catalasa (CAT), superóxido dismutasa (SOD) y glutatión peroxidasa (GPx) tras exponer las células durante 24 h en las células CHO-K1 y HepG2. Los resultados mostraron una disminución de los niveles de GSH y de la actividad de la catalasa. Mientras que se evidenció un aumento en la actividad de la SOD y GPx. Todos estos efectos tóxicos pueden conducir a un riesgo en la salud humana. Advisors/Committee Members: Ruiz Leal, María José (advisor).

Subjects/Keywords: zearalenona; CHO-K1; HepG2; citotoxicidad

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APA (6^th Edition):

Tatay Dualde, E. (2017). Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/61140

Chicago Manual of Style (16^th Edition):

Tatay Dualde, Elena. “Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares .” 2017. Doctoral Dissertation, Universitat de Valencia. Accessed January 22, 2021. http://hdl.handle.net/10550/61140.

MLA Handbook (7^th Edition):

Tatay Dualde, Elena. “Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares .” 2017. Web. 22 Jan 2021.

Vancouver:

Tatay Dualde E. Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10550/61140.

Council of Science Editors:

Tatay Dualde E. Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares . [Doctoral Dissertation]. Universitat de Valencia; 2017. Available from: http://hdl.handle.net/10550/61140

3. Lopes, Edgar Silva. Caracterização química e avaliação da atividade antioxidante de Coriandrum sativum.

Degree: 2014, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2941

►

Dissertação de Mestrado em Biotecnologia e Qualidade Alimentar

Cada vez mais se investe na melhoria da nossa saúde e bem-estar, pelo que ter uma alimentação… (more)

▼

Dissertação de Mestrado em Biotecnologia e Qualidade Alimentar

Cada vez mais se investe na melhoria da nossa saúde e bem-estar, pelo que ter uma alimentação saudável tem vindo a adquirir importância para a realização deste objetivo. Desta forma, achou-se interessante avaliar as potencialidades de diversos alimentos na contribuição de compostos bioativos que têm demonstrado in vivo a sua eficácia e ação benéfica. Assim, este estudo centrou as suas atenções num conjunto aleatório de alimentos da dieta tradicional mediterrânica para, numa fase inicial, através de ensaios preliminares evidenciar algum potencial num número restrito deles, para desta forma selecionar um para caracterizar de forma mais exaustiva. Com base no rastreio inicial, selecionaram-se os extratos de folhas e sementes de coentro, e foram aplicados vários testes para avaliar os seus efeitos antioxidantes, nomeadamente pela captação do ião superóxido, capacidade da inibição da hemólise de eritrócito, inibição da peroxidação lipídica, bem como pelo ensaio com ácido cis-parinárico e pelo ensaio dos TBARS. Os resultados obtidos mostraram que os coentros são uma planta com excelentes atividades antioxidantes e que as sementes parecem possuir um bom potencial hepatoprotetor. Nos ensaios realizados o extrato de semente foi o que apresentou maior atividade antioxidante em qualquer um dos diferentes testes aplicados. Aparentemente também foi o que protegeu mais as células HepG2 da toxicidade induzida pelo tert-butil hidroperóxido. Isto é suportado pela menor alteração nos dois biomarcadores utilizados (catalase e glutatião S-transferase). Contudo, serão necessários ensaios adicionais, com outro desenho experimental, para que se possa melhor compreender o verdadeiro potencial citoprotetor deste extrato.

Taking into account that increasingly search for ways to improve our health, and that the concept of good diet has gained importance. For the achievement of this objective, it is necessary to evaluate the potential of various foods that can contribute with bioactive compounds. For this reason, this study focused his attentions on a random set of traditional Mediterranean diet foods at an early stage, for through preliminary tests show some potential on a limited number of them to choose from among these the extract obtained with more accurate techniques. Coriander (leaves and seeds) were selected for further studies. A several numerous of methods to assess their antioxidant activity was performed. Namely, scavenging of superoxide ion, ability of inhibition of erythrocyte hemolysis, inhibition of lipid peroxidation by two different methods (cis-parinaric and TBARS). The results showed that the coriander plant show excellent antioxidant activities and the seeds seem to have a good hepatoprotective potential. Seeds extract showed the highest antioxidant activity in any one of the different tests made. Apparently coriander seed extract was also the most efficient in protecting HepG2 cells against tert-butyl hydroperoxide induced toxicity. This is supported by…

Advisors/Committee Members: Sintra, Ana Lúcia, Peixoto, Francisco.

Subjects/Keywords: Polifenol; Coentro; Stresse oxidativo; HepG2; tert-BHP

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APA (6^th Edition):

Lopes, E. S. (2014). Caracterização química e avaliação da atividade antioxidante de Coriandrum sativum. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2941

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lopes, Edgar Silva. “Caracterização química e avaliação da atividade antioxidante de Coriandrum sativum.” 2014. Thesis, RCAAP. Accessed January 22, 2021. http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2941.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lopes, Edgar Silva. “Caracterização química e avaliação da atividade antioxidante de Coriandrum sativum.” 2014. Web. 22 Jan 2021.

Vancouver:

Lopes ES. Caracterização química e avaliação da atividade antioxidante de Coriandrum sativum. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Jan 22]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2941.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lopes ES. Caracterização química e avaliação da atividade antioxidante de Coriandrum sativum. [Thesis]. RCAAP; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2941

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Froder, Juliano Gabriel [UNESP]. Estudo da genotoxicidade e citotoxicidade da indometacina nanoencapsulada in vitro.

Degree: 2017, Universidade Estadual Paulista

URL: http://hdl.handle.net/11449/147077

► Os anti-inflamatórios não esteroides (NSAIDs) estão entre os medicamentos mais amplamente utilizados no mundo inteiro. Isso se deve à medicina curativa implantada principalmente no ocidente.… (more)

▼ Os anti-inflamatórios não esteroides (NSAIDs) estão entre os medicamentos mais amplamente utilizados no mundo inteiro. Isso se deve à medicina curativa implantada principalmente no ocidente. Com frequência os NSAIDs são usados para aliviar queixas musculoesqueléticas, artrite, bursite, dor de dentes, dismenorreia, dor de estados pós-parto e na dor de metástases de câncer no osso, sendo todas essas enfermidades associadas ao aumento da síntese de prostaglandinas. Os efeitos farmacológicos dos NSAIDs são devidos à inibição da ciclooxigenase (COX) e a subsequente diminuição da síntese de prostaglandinas (PGs), levando a uma diminuição da inflamação, dor e febre. Essa diminuição de PGs leva a uma larga escala de efeitos colaterais que são associados à NSAIDs, incluindo complicações gastrointestinais (CGI), problemas cardiovasculares, toxicidade renal, hipertensão e retenção de líquidos. Devido a essas características dos NSAIDs, justificam-se estudos envolvendo a utilização de outros meios para a entrega oral de fármacos anti-inflamatórios. Uma opção muito pesquisada, que vem crescendo de maneira significativa é o uso de nanopartículas, com potencial para constituir uma nova geração de “sistemas de entrega de drogas”. Nanopartículas apresentam uma série de características, tais como: detectar, monitorar e tratar doenças; proteger o fármaco de ácidos estomacais e enzimas do trato gastrointestinal; e proteger o estômago e intestino do próprio fármaco. A indometacina é um poderoso agente NSAID, utilizado para tratamento de artrite e osteoartrite. Como inibidora não seletiva da ciclooxigenase, principalmente no caso de inibição da ciclooxigenase-2 (COX-2), que é muito relacionada às lesões da mucosa gástrica. Por esta razão, é de grande importância que a indometacina seja objeto de estudo para novos métodos e formas de distribuição desse fármaco. Considerando que não há dados na literatura sobre a análise da biossegurança do uso da Indometacina Nanoencapsulada (NI) sobre o material genético e sua toxicidade em células de mamíferos, este estudo tem por objetivo avaliar a viabilidade celular deste medicamento nanoencapsulado pelo teste de coloração com o azul de Tripan, e sua citotoxicidade pelo ensaio do MTT, e sua genotoxicidade por intermédio do ensaio cometa e teste do micronúcleo com bloqueio na citocinese. Todos os ensaios foram feitos com o uso de culturas de linfócitos humanos de sangue periférico (células não metabolizadoras) e de células do hepatoma humano (HepG2) (células metabolizadoras) (exceto o MTT). Os ensaios dos testes de viabilidade foram realizados com tempo de exposição à substância teste por 24 horas, com as seguintes concentrações: 5, 10, 50, 125, 250, 500 e 1250 μg/ml. Os resultados obtidos permitiram a seleção de seis concentrações (5, 10, 50, 125, 250 e 500 μg/ml) 11 onde foi observado uma viabilidade celular ≥ 80%. Também foi possível realizar o ensaio de citotoxicidade utilizando as células HepG2, com as mesmas concentrações e pelo mesmo período de tempo usado no teste de exclusão azul de tripan.… Advisors/Committee Members: Maistro, Edson Luis [UNESP], Universidade Estadual Paulista (UNESP).

Subjects/Keywords: Genotoxicidade; HepG2; Linfócitos humanos; Indometacina nanoencapsulada

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Froder, J. G. [. (2017). Estudo da genotoxicidade e citotoxicidade da indometacina nanoencapsulada in vitro. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/147077

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Froder, Juliano Gabriel [UNESP]. “Estudo da genotoxicidade e citotoxicidade da indometacina nanoencapsulada in vitro.” 2017. Thesis, Universidade Estadual Paulista. Accessed January 22, 2021. http://hdl.handle.net/11449/147077.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Froder, Juliano Gabriel [UNESP]. “Estudo da genotoxicidade e citotoxicidade da indometacina nanoencapsulada in vitro.” 2017. Web. 22 Jan 2021.

Vancouver:

Froder JG[. Estudo da genotoxicidade e citotoxicidade da indometacina nanoencapsulada in vitro. [Internet] [Thesis]. Universidade Estadual Paulista; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/11449/147077.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Froder JG[. Estudo da genotoxicidade e citotoxicidade da indometacina nanoencapsulada in vitro. [Thesis]. Universidade Estadual Paulista; 2017. Available from: http://hdl.handle.net/11449/147077

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Tarhan, Seda. ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi .

Degree: ESOGÜ, Tıp Fakültesi, Tıbbi Biyokimya, 2013, Eskisehir Osmangazi University

URL: http://hdl.handle.net/11684/691

► Bu çalışmada ε-viniferinin tek başına ve vinkristin sülfat kombine uygulamasının HepG2 hücrelerinde lipid peroksidasyonu (LPO), süperoksit dismutaz (SOD) ve redükte glutatyon (GSH) düzeylerine doğrudan ve… (more)

▼ Bu çalışmada ε-viniferinin tek başına ve vinkristin sülfat kombine uygulamasının HepG2 hücrelerinde lipid peroksidasyonu (LPO), süperoksit dismutaz (SOD) ve redükte glutatyon (GSH) düzeylerine doğrudan ve H2O2 ile dışsal kaynaklı oluşturulmuş oksidatif stres üzerine koruyucu etkilerinin araştırılması amaçlanmıştır. Çalışmamız birinci aşamada, maddelerin düşük ve yüksek dozlarından oluşturulmuş dVNF, yVNF, dVCR, yVCR, dETO, yETO, d(VCR+VNF), y(VCR+VNF) grupları ile Kontrol grubundan ve ikinci aşamada bu gruplara ek olarak dışsal kaynaklı oksidatif strese karşı maddelerin koruyucu etkilerini gözlemleyebilmek için kullanılan H2O2-Kontrol grubundan oluşturulmuştur. Çalışmamızda sıvı azot tankından çıkarılan hücreler besiyerinde çoğaltılmıştır. Hücreler yeterli sayıya ulaştıktan sonra maddelerin düşük ve yüksek dozları ile 3, 6 ve 24 saat inkübe edilmişlerdir. Antioksidan özelliklerini incelediğimiz maddelerin LPO ve SOD ölçümleri deney kitleri ile kolorometrik olarak ölçülmüş sonuçlar absorbansa karşı değerlendirilmiştir. GSH ölçümleri ise flourometrik deney kiti ile ölçülerek sonuçlar floresans ışımasına karşı değerlendirilmiştir. Sonuç olarak, birinci aşamada vinkristin sülfat zamana ve doza bağımlı olarak LPO SOD ve GSH değerleri üzerinde kontrole göre anlamlı bir etki göstermemiştir. ε-viniferin ile kombine uyguladığımız ise LPO ve GSH düzeylerinde kontrole göre anlamlı bir etki göstermemiş ancak SOD aktivasyonunu kontrole göre anlamlı olarak azaltmıştır (p<0,05). Elde ettiğimiz verilere göre, zamana bağımlı olarak kombine uygulamanın HepG2 hücrelerinde SOD aktivasyonunu azaltarak hücresel oksidatif stresi arttırabileceğini ve hepatoma hücrelerinde hasar yaratacağını düşünüyoruz.İkinci aşamada ise düşük doz kombine uygulamanın tüm parametrelerde artışa yol açarken, yüksek doz kombine uygulamanın H2O2 ile uyarılmış LPO düzeylerini etkilemeden SOD aktivasyonunu ve GSH düzeylerini arttırması kombine uygulamanın HepG2 hücreleri üzerinde dışsal oksidatif strese karşı koruyucu etkisi olduğunu göstermektedir. Advisors/Committee Members: Sütken Demirkan, Emine (advisor).

Subjects/Keywords: ε-viniferin; HepG2; LPO; SOD; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tarhan, S. (2013). ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi . (Thesis). Eskisehir Osmangazi University. Retrieved from http://hdl.handle.net/11684/691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tarhan, Seda. “ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi .” 2013. Thesis, Eskisehir Osmangazi University. Accessed January 22, 2021. http://hdl.handle.net/11684/691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tarhan, Seda. “ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi .” 2013. Web. 22 Jan 2021.

Vancouver:

Tarhan S. ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi . [Internet] [Thesis]. Eskisehir Osmangazi University; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/11684/691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tarhan S. ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi . [Thesis]. Eskisehir Osmangazi University; 2013. Available from: http://hdl.handle.net/11684/691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Norte

6. Cunha, Dayse Caroline Severiano da. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers .

Degree: 2010, Universidade do Rio Grande do Norte

URL: http://repositorio.ufrn.br/handle/123456789/12566

► A 140,0 kDa lectin was purified and characterized from the mushroom Clavaria cristata. The purification procedures from the crude extract of the mushroom comprised gel… (more)

Subjects/Keywords: Macrófagos; Óxido nítrico; HepG2; Edema de pata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cunha, D. C. S. d. (2010). Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers . (Thesis). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/12566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cunha, Dayse Caroline Severiano da. “Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers .” 2010. Thesis, Universidade do Rio Grande do Norte. Accessed January 22, 2021. http://repositorio.ufrn.br/handle/123456789/12566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cunha, Dayse Caroline Severiano da. “Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers .” 2010. Web. 22 Jan 2021.

Vancouver:

Cunha DCSd. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers . [Internet] [Thesis]. Universidade do Rio Grande do Norte; 2010. [cited 2021 Jan 22]. Available from: http://repositorio.ufrn.br/handle/123456789/12566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cunha DCSd. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers . [Thesis]. Universidade do Rio Grande do Norte; 2010. Available from: http://repositorio.ufrn.br/handle/123456789/12566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Norte

7. Cunha, Dayse Caroline Severiano da. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers .

Degree: 2010, Universidade do Rio Grande do Norte

URL: http://repositorio.ufrn.br/handle/123456789/12566

► A 140,0 kDa lectin was purified and characterized from the mushroom Clavaria cristata. The purification procedures from the crude extract of the mushroom comprised gel… (more)

Subjects/Keywords: Macrófagos; Óxido nítrico; HepG2; Edema de pata

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cunha, D. C. S. d. (2010). Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers . (Masters Thesis). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/12566

Chicago Manual of Style (16^th Edition):

Cunha, Dayse Caroline Severiano da. “Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers .” 2010. Masters Thesis, Universidade do Rio Grande do Norte. Accessed January 22, 2021. http://repositorio.ufrn.br/handle/123456789/12566.

MLA Handbook (7^th Edition):

Cunha, Dayse Caroline Severiano da. “Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers .” 2010. Web. 22 Jan 2021.

Vancouver:

Cunha DCSd. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers . [Internet] [Masters thesis]. Universidade do Rio Grande do Norte; 2010. [cited 2021 Jan 22]. Available from: http://repositorio.ufrn.br/handle/123456789/12566.

Council of Science Editors:

Cunha DCSd. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers . [Masters Thesis]. Universidade do Rio Grande do Norte; 2010. Available from: http://repositorio.ufrn.br/handle/123456789/12566

University of Vienna

8. Klimpke, Antonia. Einfluss von Bilirubin auf den Fettstoffwechsel von HepG2-Zellen.

Degree: 2019, University of Vienna

URL: http://othes.univie.ac.at/56954/

► In der vorliegenden Arbeit sollte untersucht werden, welchen Einfluss UKB auf den Fettstoffwechsel von HepG2-Zellen hat. Hierfür wurden die Zellen mit zwei FFS-Konzentrationen (0,5mM, 1mM)… (more)

Subjects/Keywords: 44.21 Ernährung; Bilirubin / Fettstoffwechsel / HepG2-Zellen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klimpke, A. (2019). Einfluss von Bilirubin auf den Fettstoffwechsel von HepG2-Zellen. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/56954/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klimpke, Antonia. “Einfluss von Bilirubin auf den Fettstoffwechsel von HepG2-Zellen.” 2019. Thesis, University of Vienna. Accessed January 22, 2021. http://othes.univie.ac.at/56954/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klimpke, Antonia. “Einfluss von Bilirubin auf den Fettstoffwechsel von HepG2-Zellen.” 2019. Web. 22 Jan 2021.

Vancouver:

Klimpke A. Einfluss von Bilirubin auf den Fettstoffwechsel von HepG2-Zellen. [Internet] [Thesis]. University of Vienna; 2019. [cited 2021 Jan 22]. Available from: http://othes.univie.ac.at/56954/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klimpke A. Einfluss von Bilirubin auf den Fettstoffwechsel von HepG2-Zellen. [Thesis]. University of Vienna; 2019. Available from: http://othes.univie.ac.at/56954/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Shen, Minqian. Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism.

Degree: PhD, Cell, Molecular and Structural Biology (CMSB), 2017, Miami University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921

► Liver is one of the most essential organs involved in the regulation of energy homeostasis. Hepatic steatosis, a major manifestation of metabolic syndrome, is associated… (more)

▼ Liver is one of the most essential organs involved in the regulation of energy homeostasis. Hepatic steatosis, a major manifestation of metabolic syndrome, is associated with imbalance between lipid formation and breakdown and glucose production and catabolism. Although the most common risk factors of hepatocellular carcinoma (HCC) are hepatic virus infection and alcohol, a rapid increase in obesity has become a prime cause of HCC, outweighing HCC with virus- or alcohol- related etiology. Estrogens affect both cell metabolism and proliferation. Both nuclear estrogen receptors (ERs), ER-a and ER-ß, are expressed in the liver. How estrogen hormones regulate liver homeostasis and which receptors play the fundamental role in the metabolic process are not clear. Moreover, whether estrogen hormones and ERs are protective or detrimental in HCC is under debate, and whether estrogens can alter HCC metabolism and interfere with leptin-induced HCC is not known. The goal of my dissertation is to further elucidate the roles of estrogens and ERs in normal liver metabolism as well as HCC metabolism and proliferation using both in vivo and in vitro models. Thus, I hypothesize that 1) exogenous estrogen replacement reverses liver metabolic alteration in estrogen-depleted mice mainly through ER-a receptor; 2) estrogens inhibit leptin-induced HepG2 cell proliferation mainly through ER-ß activation and alter HepG2 metabolism mainly through ER-a activation. In this dissertation, previous studies on the functions of estrogen and estrogen receptors in liver and liver cancer are discussed in Chapter 1. Studies using ovariectomized mouse model with hormone-replacement was applied to determine the role of estrogens and estrogen receptors in mouse liver metabolism are discussed in Chapter 2. Human cancer cell line HepG2 was treated with different concentrations of estrogen and estrogen receptor agonists as well as estrogen receptor siRNA to determine the role of estrogen and estrogen receptors on HepG2 cancer cell proliferation, apoptosis and leptin signal pathway in Chapter 3. High performance liquid chromatography (HPLC) was used to determine the role of estrogen and estrogen receptors on HepG2 cancer cell gene expression and metabolic profiles in Chapter 4. In Chapter Five, I conclude the finding of my current studies and give perspectives for future research directions of estrogen hormones and estrogen receptors in the regulation of liver and liver cancer metabolism. Advisors/Committee Members: Shi, Haifei (Advisor), Killian, Kathleen (Committee Chair).

Subjects/Keywords: Biology; Estrogen; Estrogen receptors; Liver; HepG2; Obesity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shen, M. (2017). Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism. (Doctoral Dissertation). Miami University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921

Chicago Manual of Style (16^th Edition):

Shen, Minqian. “Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism.” 2017. Doctoral Dissertation, Miami University. Accessed January 22, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921.

MLA Handbook (7^th Edition):

Shen, Minqian. “Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism.” 2017. Web. 22 Jan 2021.

Vancouver:

Shen M. Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism. [Internet] [Doctoral dissertation]. Miami University; 2017. [cited 2021 Jan 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921.

Council of Science Editors:

Shen M. Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism. [Doctoral Dissertation]. Miami University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921

10. França, Carla Fernanda Baquedano. Quantificação de lesões em DNA e RNA em cultura de células expostas a tetraidrofurano.

Degree: Mestrado, Toxicologia e Análises Toxicológicas, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/9/9141/tde-13032013-141828/ ;

►

Tetraidrofurano (THF) é um solvente muito utilizado industrialmente, com demonstrada ação carcinogênica em animais experimentais, mas pouco se sabe sobre sua toxicidade celular, danos a… (more)

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Tetraidrofurano (THF) é um solvente muito utilizado industrialmente, com demonstrada ação carcinogênica em animais experimentais, mas pouco se sabe sobre sua toxicidade celular, danos a biomoléculas e genotoxicidade. Em trabalho anterior realizado no laboratório, foi verificado que adutos de DNA são formados a partir da reação de THF oxidado com 2\'-desoxiguanosina (dGuo), 2\'-desoxiadenosina (dAdo) e 2\'-desoxicitidina (dCyd) in vitro. A ocorrência dessas lesões em sistemas biológicos expostos a THF nunca foi demonstrada e pode indicar uma via de ação genotóxica desse solvente, até o momento não considerada nos estudos de carcinogênese e toxicidade. Neste trabalho foi investigada a indução de lesões em DNA e RNA de sistemas biológicos expostos ao THF, assim como alterações epigenéticas. Como sistemas biológicos foram utilizados homogenato de fígado de camundongos, cultura de células HepG2 e fígado e rim de camundongos que foram, em trabalho anterior, expostos a vapores do solvente. Métodos de HPLC-ESI-MS/MS foram validados para quantificação de todas as lesões em DNA. Foi verificado que THF é um solvente de baixa citoxicidade para células HepG2, sendo necessárias concentrações acima de 50 mM por período de incubação superior a 24 h para ser observada perda de viabilidade. Houve indução de dano oxidativo em DNA de células HepG2 e de fígado de camundongos expostos. Uma vez que o solvente esteja oxidado, a espécie reativa THF-OH presente no meio de cultura é capaz de reagir com RNA e DNA das células e DNA de homogenato de fígado de camundongos, gerando adutos com dAdo, dGuo e Ado. A espécie reativa possui maior afinidade por dGuo que por dAdo no DNA. Entretanto, as células HepG2 não biotransformaram THF para o intermediário reativo. Além disso, THF induziu hipermetilação no DNA das células HepG2 expostas a 50 e 100 mM do solvente e em DNA de fígado de camundongos fêmeas C57BL/6J expostos a vapores de THF (vapor de 1 mL/h, 6h/dia, 5 dias). Este estudo mostra pela primeira vez a reatividade do solvente THF oxidado (contendo THF-OH) com DNA e RNA em sistemas biológicos, assim como alteração epigenética induzida pelo solvente, e servirá para uma melhor compreensão dos mecanismos envolvidos na indução de câncer por THF.

Tetrahydrofuran (THF) is a widely used industrial solvent with demonstrated carcinogenic action in experimental animals, but little is known about its cellular toxicity and genotoxic damage to biomolecules. Previous work of this group showed that DNA adducts are formed from the reaction of oxidized THF with 2\'-deoxyguanosine (dGuo), 2\'-deoxyadenosine (dAdo), and 2\'-deoxycytidine (dCyd) in vitro. The occurrence of these lesions in biological systems exposed to THF has never been demonstrated and may indicate a genotoxic pathway of this solvent, so far not considered in carcinogenesis and toxicity studies. It was investigated here the induction of DNA and RNA lesions in biological systems exposed to THF, as well as epigenetic changes. The biological systems used were mice liver homogenate, HepG2 cell…

Advisors/Committee Members: Loureiro, Ana Paula de Melo.

Subjects/Keywords: Adutos de DNA; DNA adducts; Estresse oxidativo; HepG2; HepG2; Oxidative stress; Tetrahydrofuran; Tetraidrofurano

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

França, C. F. B. (2012). Quantificação de lesões em DNA e RNA em cultura de células expostas a tetraidrofurano. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/9/9141/tde-13032013-141828/ ;

Chicago Manual of Style (16^th Edition):

França, Carla Fernanda Baquedano. “Quantificação de lesões em DNA e RNA em cultura de células expostas a tetraidrofurano.” 2012. Masters Thesis, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/9/9141/tde-13032013-141828/ ;.

MLA Handbook (7^th Edition):

França, Carla Fernanda Baquedano. “Quantificação de lesões em DNA e RNA em cultura de células expostas a tetraidrofurano.” 2012. Web. 22 Jan 2021.

Vancouver:

França CFB. Quantificação de lesões em DNA e RNA em cultura de células expostas a tetraidrofurano. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/9/9141/tde-13032013-141828/ ;.

Council of Science Editors:

França CFB. Quantificação de lesões em DNA e RNA em cultura de células expostas a tetraidrofurano. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/9/9141/tde-13032013-141828/ ;

11. Vincourt-Vitse, Véronique. Contribution à la formulation et à l'évaluation de liposomes d'ATP : Contribution to the formulation and the evaluation of ATP liposomes.

Degree: Docteur es, Pharmacologie, 2012, Université Paris Descartes – Paris V

URL: http://www.theses.fr/2012PA05P651

►

Les liposomes d’ATP incluant des ligands hépatiques pourraient contribuer à améliorer le statut énergétique du greffon hépatique. Une première phase de développement a mis en… (more)

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Les liposomes d’ATP incluant des ligands hépatiques pourraient contribuer à améliorer le statut énergétique du greffon hépatique. Une première phase de développement a mis en évidence la nécessité de stabiliser la forme (i) et de valider un modèle cellulaire à statut énergétique altéré (ii) afin de pouvoir tester différentes formulations liposomales d’intérêt. Afin de résoudre la première problématique, différentes stratégies ont été mises en place lors de la lyophilisation de liposomes blancs ou chargés en ATP. Le saccharose et le tréhalose ont conduit à une stabilisation de la forme avant et après lyophilisation. Néanmoins, quel que soit le procédé, la lyophilisation s’est accompagnée d’une fuite en ATP. L’ajout d’un agent inhibant la cartinine palmitoyl transferase, l’Etomoxir, s’est révélé un modèle intéressant pour moduler le niveau énergétique des HepG2 en fonction de la température et de la concentration utilisée. En conclusion, ce travail contribue à mieux comprendre les facteurs critiques liés à la lyophilisation des liposomes (i) et décrit des modèles cellulaires hépatiques à statut énergétique altéré qui pourraient être mis à profit pour tester différents principes actifs ou formes galéniques innovantes.

ATP liposome incorporating hepatic ligands may contribute to improve the energetic status of the liver graft. In a first phase of development, it has been emphasized the great need of stabilizing the liposome (i) and of validating a cellular model with an altered energetic status in order to test the formulations of interest. To provide a stable liposomal preparation, different strategies have been carried out to freeze-dry liposome with or without ATP. Sucrose and trehalose better stabilize the liposome preparation during the freeze-drying process. Nevertheless, in all conditions, a significant ATP leakage has been observed. An inhibitor of the cartinine palmitoyl transferase (i.e. Etomoxir) has shown great interest to modulate the energetic level in HepG2 cells by varying Etomoxir concentration and culture temperature. In conclusion, this study contributes to a better understanding of the critical factors related to liposome freeze-drying and describes hepatic cell models with altered energetic status that may have great interest to test specific agent or innovating formulations.

Advisors/Committee Members: Dumortier, Gilles (thesis director).

Subjects/Keywords: ATP; Liposome; Lyophilisation; HepG2; Etomoxir; ATP; Liposome; Freeze drying; HepG2; Etomoxir; 612.015

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vincourt-Vitse, V. (2012). Contribution à la formulation et à l'évaluation de liposomes d'ATP : Contribution to the formulation and the evaluation of ATP liposomes. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2012PA05P651

Chicago Manual of Style (16^th Edition):

Vincourt-Vitse, Véronique. “Contribution à la formulation et à l'évaluation de liposomes d'ATP : Contribution to the formulation and the evaluation of ATP liposomes.” 2012. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed January 22, 2021. http://www.theses.fr/2012PA05P651.

MLA Handbook (7^th Edition):

Vincourt-Vitse, Véronique. “Contribution à la formulation et à l'évaluation de liposomes d'ATP : Contribution to the formulation and the evaluation of ATP liposomes.” 2012. Web. 22 Jan 2021.

Vancouver:

Vincourt-Vitse V. Contribution à la formulation et à l'évaluation de liposomes d'ATP : Contribution to the formulation and the evaluation of ATP liposomes. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2012. [cited 2021 Jan 22]. Available from: http://www.theses.fr/2012PA05P651.

Council of Science Editors:

Vincourt-Vitse V. Contribution à la formulation et à l'évaluation de liposomes d'ATP : Contribution to the formulation and the evaluation of ATP liposomes. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2012. Available from: http://www.theses.fr/2012PA05P651

12. Dorta, Daniel Junqueira. Efeitos citoprotetor e/ou citotóxico dos flavonóides: estudo estrutura-atividade envolvendo mecanismos mitocondriais, com ênfase na apoptose.

Degree: PhD, Toxicologia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/60/60134/tde-17102007-160656/ ;

►

Realizou-se um estudo estrutura-atividade sobre os efeitos citoprotetor/citotóxico de 5 flavonóides envolvendo processos mitocondriais, com ênfase na apoptose. Nossos resultados mostram que a dupla ligação… (more)

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Realizou-se um estudo estrutura-atividade sobre os efeitos citoprotetor/citotóxico de 5 flavonóides envolvendo processos mitocondriais, com ênfase na apoptose. Nossos resultados mostram que a dupla ligação na posição 2-3 / grupos 3-OH em conjugação com a função 4-oxo no anel C da estrutura dos flavonóides parece favorecer a interação destes compostos com a membrana mitocondrial, diminuindo a sua fluidez, e tanto inibindo a cadeia respiratória das mitocôndrias, quanto causando desacoplamento. Por outro lado, a estrutura o-di-OH no anel B parece favorecer a inibição da cadeia respiratória, sendo que a ausência desta estrutura parece favorecer a atividade desacopladora. Os flavonóides que não afetaram a respiração mitocondrial, induziram a transição de permeabilidade mitocondrial. A capacidade dos flavonóides em liberar o Ca2+ acumulado pelas mitocôndrias correlaciona-se com a sua capacidade de afetar a respiração mitocondrial e sua inabilidade em induzir a transição de permeabilidade mitocondrial. Já os dados referentes aos estudos da atividade protetora contra a formação de radicais livres demonstraram que a quercetina, luteolina e a galangina foram substancialmente mais potentes que a taxifolina e a catequina em conferir proteção contra a lipoperoxidação, embora somente a quercetina tenha sido um efetivo seqüestrador tanto de DPPH?, quanto de O2?-. Esses resultados sugerem que a dupla ligação na posição 2-3 em conjugação com a função 4-oxo na estrutura dos flavonóides são os fatores mais importantes na atividade antioxidante dos flavonóides sobre as mitocôndrias. Ainda, a presença da estrutura o-di-OH no anel B, conforme observado na quercetina, favorece essa atividade via seqüestro de O2?-, enquanto que a ausência dessa característica estrutural na galangina, a favorece via diminuição da fluidez de membrana e/ou desacoplamento mitocondrial. Os ensaios realizados para avaliar o efeito dos flavonóides sobre as células HepG2 mostraram que galangina, luteolina e quercetina são capazes de induzir morte celular. Esse efeito parece estar associado à dissipação do potencial de membrana mitocondrial e à diminuição na capacidade energética celular, mais pronunciada no caso dos dois primeiros; porém, como essa diminuição na concentração de ATP não é drástica, a morte celular pode ocorrer por apoptose. Os experimentos realizados para avaliar essa possibilidade sugerem uma ativação das caspases via mitocôndria, observada pelo aumento das atividades de caspase -9 e -3 e também pela exposição de fosfatidil serina. A taxifolina que não apresentou capacidade de produzir dano celular, apresentou, por outro lado, capacidade de prevenir parcialmente a diminuição de viabilidade das células HepG2 induzida pelo pró-oxidante t-butilhidroperóxido, aparentemente por meio de sua atividade antioxidante que inibiu, também de forma parcial, o acúmulo de espécies reativas de oxigênio.

We carried out a structure-activity study addressing the protective/toxic effects of 5 flavonoids (quercetin, taxifolin, luteolin, catechin and galangin) upon…

Advisors/Committee Members: Curti, Carlos.

Subjects/Keywords: apoptose; apoptosis; células HepG2; estudo estrutura-atividade; flavonóides; Flavonoids; HepG2 cells; mitochondria; mitocôndria; necrose; necrosis; structure-activity study

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dorta, D. J. (2007). Efeitos citoprotetor e/ou citotóxico dos flavonóides: estudo estrutura-atividade envolvendo mecanismos mitocondriais, com ênfase na apoptose. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/60/60134/tde-17102007-160656/ ;

Chicago Manual of Style (16^th Edition):

Dorta, Daniel Junqueira. “Efeitos citoprotetor e/ou citotóxico dos flavonóides: estudo estrutura-atividade envolvendo mecanismos mitocondriais, com ênfase na apoptose.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/60/60134/tde-17102007-160656/ ;.

MLA Handbook (7^th Edition):

Dorta, Daniel Junqueira. “Efeitos citoprotetor e/ou citotóxico dos flavonóides: estudo estrutura-atividade envolvendo mecanismos mitocondriais, com ênfase na apoptose.” 2007. Web. 22 Jan 2021.

Vancouver:

Dorta DJ. Efeitos citoprotetor e/ou citotóxico dos flavonóides: estudo estrutura-atividade envolvendo mecanismos mitocondriais, com ênfase na apoptose. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/60/60134/tde-17102007-160656/ ;.

Council of Science Editors:

Dorta DJ. Efeitos citoprotetor e/ou citotóxico dos flavonóides: estudo estrutura-atividade envolvendo mecanismos mitocondriais, com ênfase na apoptose. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/60/60134/tde-17102007-160656/ ;

13. Silva, Tereza Cristina da. Efeitos anti-neoplásicos da raiz de Pfaffia paniculata (Ginseng brasileiro) no modelo de hepatocarcinogênese murina e em cultura de células de hepatocarcinoma humano.

Degree: PhD, Patologia Experimental e Comparada, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10133/tde-02062008-095506/ ;

► A raiz pulverizada de Pfaffia paniculata e seu extrato butanólico apresentam propriedades antineoplásicas, quimiopreventivas e antiangiogênicas, onde muitos indícios conferem às saponinas triterpenóides presentes nestas… (more)

▼ A raiz pulverizada de Pfaffia paniculata e seu extrato butanólico apresentam propriedades antineoplásicas, quimiopreventivas e antiangiogênicas, onde muitos indícios conferem às saponinas triterpenóides presentes nestas raízes as propriedades antitumorais observadas. Sabe-se que saponinas de diversos tipos de plantas possuem capacidade de interferir diretamente no ciclo celular de células tumorais. Assim, considerando os efeitos inibitórios das raízes e extratos de P. paniculata sobre a proliferação celular, o objetivo deste trabalho foi compreender os mecanismos envolvidos na ação quimiopreventiva desta raiz, tanto na fase de iniciação da carcinogênese hepática, quanto sobre células tumorais já estabelecidas. Inicialmente, foram avaliados os efeitos de diferentes concentrações da raiz em pó de P. paniculata adicionada à ração em camundongos submetidos ao modelo de hepatocarcinogênese, pela análise da proliferação celular, indução da apoptose e a comunicação intercelular hepática. Seqüencialmente, foram avaliados os efeitos das frações purificadas do extrato butanólico destas raízes sobre linhagem de células de carcinoma hepatocelular humano. Neste experimento foram analisadas a influência do tratamento sobre a viabilidade celular, fases e proteínas do ciclo celular, proliferação e presença de morte celular. No modelo de hepatocarcinogênese o tratamento com a raiz reduziu a proliferação celular, aumentou a apoptose e desencadeou processo inflamatório crônico em intensidades dependentes da concentração testada e não afetou a comunicação intercelular. Estes resultados indicam que os efeitos quimiopreventivos da P. Paniculata são decorrentes do controle da proliferação celular e apoptose, e são diretamente influenciados pela concentração da raiz. No experimento in vitro o tratamento reduziu a concentração das células vivas sem aumentar a concentração de células mortas, diminuiu a porcentagem de células na fase G2 do ciclo celular, reduziu a expressão dos genes das proteínas ciclinas D1, E e CDK6 e aumentou a expressão de p27, e não induziu apoptose. Estes resultados mostraram que a redução na concentração de células observadas após o tratamento com a fração do extrato butanólico de P. paniculata não foi decorrente de indução de apoptose. O tratamento parou o ciclo celular das células tumorais HepG2 em G1, inibindo proteínas importantes para a progressão do ciclo e estimulando a expressão de p27 um conhecido gene inibidor de CDKs. Os efeitos antiproliferativos observados no experimento in vivo em camundongos, reproduziram-se in vitro em células tumorais humanas, o que pode indicar que as propriedades antineoplásicas anteriormente observadas não são espécie específica. Em linhas gerais, as raízes e/ou as frações do extrato butanólico obtido a partir das raízes de P. paniculata apresentam propriedades antineoplásicas por inibir a proliferação celular e induzir apoptose in vivo e por parar o ciclo celular in vitro. Estes resultados consagram as propriedades antineoplásicas de Pfaffia paniculata e motivam o desenvolvimento… Advisors/Committee Members: Dagli, Maria Lucia Zaidan.

Subjects/Keywords: Cell Cycle; Cell Proliferation; Ciclo Celular; HepG2; HepG2; Medicinal plants; Neoplasias; Neoplasias; Plantas medicinais; Proliferação Celular

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, T. C. d. (2008). Efeitos anti-neoplásicos da raiz de Pfaffia paniculata (Ginseng brasileiro) no modelo de hepatocarcinogênese murina e em cultura de células de hepatocarcinoma humano. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10133/tde-02062008-095506/ ;

Chicago Manual of Style (16^th Edition):

Silva, Tereza Cristina da. “Efeitos anti-neoplásicos da raiz de Pfaffia paniculata (Ginseng brasileiro) no modelo de hepatocarcinogênese murina e em cultura de células de hepatocarcinoma humano.” 2008. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/10/10133/tde-02062008-095506/ ;.

MLA Handbook (7^th Edition):

Silva, Tereza Cristina da. “Efeitos anti-neoplásicos da raiz de Pfaffia paniculata (Ginseng brasileiro) no modelo de hepatocarcinogênese murina e em cultura de células de hepatocarcinoma humano.” 2008. Web. 22 Jan 2021.

Vancouver:

Silva TCd. Efeitos anti-neoplásicos da raiz de Pfaffia paniculata (Ginseng brasileiro) no modelo de hepatocarcinogênese murina e em cultura de células de hepatocarcinoma humano. [Internet] [Doctoral dissertation]. University of São Paulo; 2008. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10133/tde-02062008-095506/ ;.

Council of Science Editors:

Silva TCd. Efeitos anti-neoplásicos da raiz de Pfaffia paniculata (Ginseng brasileiro) no modelo de hepatocarcinogênese murina e em cultura de células de hepatocarcinoma humano. [Doctoral Dissertation]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/10/10133/tde-02062008-095506/ ;

Universidade Federal de Viçosa

14. Marcelo Augusto Filardi. Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2.

Degree: 2010, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3200

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The hepatocellular carcinoma is the most common adult primary liver câncer and it has already become the fifth most common neoplasia in the world and… (more)

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The hepatocellular carcinoma is the most common adult primary liver câncer and it has already become the fifth most common neoplasia in the world and the third in number of deaths related to cancer. It is estimated that 2/3 of all human neoplasias could be prevented by a change in lifestyle, including a diet. The propolis, elaborated from various plant parts by Apis mellifera bees, present pharmacological properties effective in cancer treatment by biological activity attributed to its chemical components, mainly with the Brazilian propolis. More than 300 propolis compounds have been identified. Recently pharmacological studies, in which scientific interest is focused on the acetogenins, a compound class derived from fatty acids and presenting a strong anti-tumorous activity. Three experiments were carried out in the Animal Molecular Infectology Lab, in the Biochemistry and Molecular Biology Department, in the Federal University of Viçosa, for testing in vitro, the anti-tumorous properties of soursop extracts (Annona muricata) and green and red propolis extracts. For so, tumorous hepatic cells from the HepG2 lineage were used, cultivated in four different concentrations of soursop extracts (1, 2, 4 e 8 mg/mL) and red propolis (25, 50, 100 and 200 μg/mL) and green extracts (50, 100, 200 and 400 μg/mL), with espectrophotometry analysis of the cellular viability by the methodology of neutral red dye, in three time periods (24, 48 and 72 hours). The cells wich were exposed th the treatments showed strong inhibition to proliferation, mainly in the concentration of 8 mg/mL of soursop extract (86% after 24 hours and 95% after 48 hours), 200 μg/mL of red propolis extract (92% after 24 hours and 100% after 48 hours) and 400 μg/mL of green propolis extract (92% after 72 hours), which caused visible alterations in the cellular architecture as well as in the single-layer cultivation organization, showing abundant apoptotic forms. The chromatographic analysis of the chemical profile of green and red propolis extracts revealed the presence of flavonoids and other compounds with known anti-tumorous effect, such as artepellin C, pinocembrin, pinobankisin, quercetina, kanpherol, chrysin, galangin and bacarin. The main conclusion to be drawn from this discussion is that the soursop (A. muricata) along with the green and red propolis are sources of cytotoxic compounds with pontentical against hepatic carcinogenics HepG2 cells.

O carcinoma hepatocelular é o mais frequente câncer primário do fígado e já se tornou a quinta neoplasia mais comum no mundo e a terceira causa de óbitos relatados por câncer. Estima-se que 2/3 das neoplasias humanas poderiam ser prevenidos pela modificação do estilo de vida, incluindo a dieta. A própolis, elaborada de diversas partes das plantas por abelhas Apis mellifera, apresenta propriedades farmacológicas efetivas no tratamento do câncer pela atividade biológica atribuída aos seus componentes químicos, principalmente a própolis brasileira. Mais de 300 compostos da própolis já foram…

Advisors/Committee Members: Abelardo Silva Júnior, Márcia Rogéria de Almeida Lamêgo, Tanus Jorge Nagem, João Paulo Viana Leite, Leandro Licursi de Oliveira, Tânia Toledo de Oliveira.

Subjects/Keywords: Extratos da própolis; Graviola; Células hepatocarcinogênicas HepG2; METABOLISMO E BIOENERGETICA; Propolis extract; Soursop; Hepatic carcinogenic HepG2 cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Filardi, M. A. (2010). Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3200

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Filardi, Marcelo Augusto. “Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2.” 2010. Thesis, Universidade Federal de Viçosa. Accessed January 22, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3200.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Filardi, Marcelo Augusto. “Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2.” 2010. Web. 22 Jan 2021.

Vancouver:

Filardi MA. Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2. [Internet] [Thesis]. Universidade Federal de Viçosa; 2010. [cited 2021 Jan 22]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3200.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Filardi MA. Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2. [Thesis]. Universidade Federal de Viçosa; 2010. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3200

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Lithuanian Veterinary Academy

15. Adomkienė, Rasa. Būtinųjų ir chlorintų riebalų rūgščių kiekio kitimo dinamika HepG2 ir 3T3-L1 linijų ląstelėse.

Degree: PhD, Zootechny, 2009, Lithuanian Veterinary Academy

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20091223_134641-94789 ;

►

Bjorn pademonstruotas ClRR pernešimas į jūros gyvūnų mitybos grandinę, leidžia tikėtis, jog ir žmogaus organizmas gali įsisavinti chlorintas riebalų rūgštis valgant žuvį ir galbūt netgi… (more)

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Bjorn pademonstruotas ClRR pernešimas į jūros gyvūnų mitybos grandinę, leidžia tikėtis, jog ir žmogaus organizmas gali įsisavinti chlorintas riebalų rūgštis valgant žuvį ir galbūt netgi chloru apdorotus maisto produktus ar geriant chlorintą vandenį. Tuo tarpu, kol kas tik su dviejų rūšių žmogaus ląstelėmis, atlikti tyrimai, prieštarauja šiai hipotezei. Šiuo metu yra tirtas tik chlorintos stearino rūgšties įsisavinimas žarnų epitelio INT 407 ir neuroblastomos SH-SY5Y ląstelėse. Pasaulinėje literatūroje nėra duomenų apie kitų ląstelių: tiek žmonių, tiek gyvūnų, sugebėjimą įsisavinti ar kaupti chlorintas riebalų rūgštis. Pirmą kartą chlorintų riebalų rūgščių įsisavinimo tyrimas atliktas su HepG2 ir 3T3-L1 ląstelėmis. Rezultatai parodė, jog ląstelės iš maitinimo terpės įsisavina chlorintas riebalų rūgštis, tačiau labai sparčiai jas metabolizuoja. Hepatomos ląstelės įterpė 34 ir 41 proc. didesnį (P<0,05) kiekį chlorintos stearino ir palmitino rūgšties nei pelių preadipocitai. HepG2 ląstelės įsisavino daugiau nei pusę, t.y. 61 proc. chlorintos palmitino rūgšties kiekio nuo pirminės šios RR koncentracijos maitinimo terpėje, kitą dalį (34 %) per 12 h inkubacijos periodą metabolizavo, o terpėje Cl2RR liko tik pėdsakai. Taip pat pirmą kartą HepG2 ląstelės inkubuotos terpėje su 9,10-heksachlortristearoilgliceroliu. Nei ląstelėse, nei maitinimo terpėje chlorintos stearino ir kitų chlorintų riebalų rūgščių nenustatyta bei nenustatyta ir įtaka kitų ne chlorintų riebalų rūgščių... [toliau žr. visą tekstą]

Chlorinated fatty acids (ClFAs) represent a major part of the total extractable chlorinated organic compounds found in fish and other marine biota. Although ClFAs have been found in fish and marine mammals for many years, there is still lack of information about there influence on live organisms, impact on cells and bioaccumulation. Recently the analysis of the embedding of the chlorinated fatty acids into mammalian cells and its impact on the physiological processes had become a hot topic, ever since the medics had recommended for the people keen on taking care of their health to use more sea and fish products, especially because of the omega-3 fatty acids found in fish composition. ClFAs have been detected both naturally occurring in biota and formed in aquatic animals because of environmental pollution from pulp bleach mills. Furthermore ClFAs have been detected in several alimentary products. The way ClFAs get in organism and their possible accumulation are not sufficiently researched yet ClFAs have been shown to influence reproduction processes and induce disturbances of cells. Despite adverse effects ClFAs are not recognized by tissues as xenobiotic and are not eliminated from organism. The incorporation and metabolism of dichloro palmitic and dichloro stearic acids in HepG2 and 3T3-L1 cell-lines were studied. Investigation showed that HepG2 and 3T3-L1 cells uptake chlorinated fatty acids from cell culture medium, however they metabolize them fast. 9,10-dichloro... [to full text]

Advisors/Committee Members: Stankevičienė, Marija (Doctoral dissertation supervisor), Stankevičius, Henrikas (Doctoral dissertation advisor), Bakutis, Bronius (Doctoral dissertation committee chair), Garmienė, Galina (Doctoral dissertation committee member), Gružauskas, Romas (Doctoral dissertation committee member), Sederevičius, Antanas (Doctoral dissertation committee member), Šarkinas, Antanas (Doctoral dissertation committee member), Juozaitienė, Vida (Doctoral dissertation opponent), Senikienė, Žibuoklė (Doctoral dissertation opponent).

Subjects/Keywords: Chlorintos riebalų rūgštys; Aplinkos tarša; HepG2 ląstelės; 3T3-L1 ląstelės; Chlorinated fatty acids; Environmental pollution; HepG2 cells; 3T3-L1 cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Adomkienė, R. (2009). Būtinųjų ir chlorintų riebalų rūgščių kiekio kitimo dinamika HepG2 ir 3T3-L1 linijų ląstelėse. (Doctoral Dissertation). Lithuanian Veterinary Academy. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20091223_134641-94789 ;

Chicago Manual of Style (16^th Edition):

Adomkienė, Rasa. “Būtinųjų ir chlorintų riebalų rūgščių kiekio kitimo dinamika HepG2 ir 3T3-L1 linijų ląstelėse.” 2009. Doctoral Dissertation, Lithuanian Veterinary Academy. Accessed January 22, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20091223_134641-94789 ;.

MLA Handbook (7^th Edition):

Adomkienė, Rasa. “Būtinųjų ir chlorintų riebalų rūgščių kiekio kitimo dinamika HepG2 ir 3T3-L1 linijų ląstelėse.” 2009. Web. 22 Jan 2021.

Vancouver:

Adomkienė R. Būtinųjų ir chlorintų riebalų rūgščių kiekio kitimo dinamika HepG2 ir 3T3-L1 linijų ląstelėse. [Internet] [Doctoral dissertation]. Lithuanian Veterinary Academy; 2009. [cited 2021 Jan 22]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20091223_134641-94789 ;.

Council of Science Editors:

Adomkienė R. Būtinųjų ir chlorintų riebalų rūgščių kiekio kitimo dinamika HepG2 ir 3T3-L1 linijų ląstelėse. [Doctoral Dissertation]. Lithuanian Veterinary Academy; 2009. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20091223_134641-94789 ;

16. Silva, Sandra Cristina Machado da. Efeito do extracto de folhas de Ginkgo biloba em células HepG2 tratadas com Paraquato.

Degree: 2013, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2825

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Dissertação de Mestrado em Genética Molecular Comparativa e Tecnológica

O extracto de folhas de Ginkgo biloba L. é um dos fármacos de origem vegetal mais… (more)

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Dissertação de Mestrado em Genética Molecular Comparativa e Tecnológica

O extracto de folhas de Ginkgo biloba L. é um dos fármacos de origem vegetal mais vendido no Mundo. Relativamente a este extracto o presente trabalho estudou a sua genotoxicidade/antigenotoxicidade e esclarecer se este detém um efeito quimioprotector contra os danos induzidos pelo agente indutor de espécies reactivas de oxigénio (ROS), o Paraquato (PQ), usando a versão alcalina do Ensaio do Cometa para medir as quebras de cadeia de DNA basais e medir as 8-oxoguaninas e outras purinas alteradas (sítios FPG) pela incubação com formamidopirimidina DNA glicosilase (FPG). O PQ é um herbicida que origina a produção de ROS dentro das células e tem sido apontado como um factor relevante no desenvolvimento da doença de Parkinson. Neste estudo analisou-se a sua genotoxicidade e pretendeu-se contribuir para o estabelecimento de um protocolo alternativo aos métodos usados para a indução de danos oxidativos. O efeito de diferentes concentrações de Paraquato (1; 1,5; 2 e 5 μM) foi testado em células HepG2. Diferentes concentrações de extracto vegetal (0,5; 5 e 10 g/L) foram testadas sozinhas ou combinadas com Paraquato (1 e 1,5 μM), ao longo do tempo (de uma a 24 horas de incubação), de modo a avaliar o efeito mutagénico/antimutagénico deste extracto vegetal. A incubação das células HepG2 com o extracto vegetal (10 g/L) durante uma hora aumentou os danos no DNA (quebras de cadeia e dano oxidativo), provavelmente devido aos ácidos ginkgólicos presentes no extracto bruto. A incubação com 0,5 g/L ao longo do tempo (uma, 4 e 24 horas) indicou que, 4 horas após a incubação das células HepG2, o dano é sobretudo oxidativo. Os tratamentos com Paraquato conduziram a um aumento do dano oxidativo no DNA, nas concentrações 1 e 1,5 μM, após 4 horas de incubação. O pré-tratamento de 24 horas das células HepG2 com Ginkgo biloba L. (0.5 e 5 g/L), antes do tratamento com PQ (1 e 1,5 μM, durante 1 hora), aumentou o dano oxidativo no DNA, revelando, ao contrario do que seria de esperar, efeitos genotóxicos quando usado nesta concentração e tempo.

Ginkgo biloba L. leaf extract is one of the most widely used nutritional supplements in the world. The aim of this work was to study the genotoxic/antigenotoxic effect of the Ginkgo biloba L in HepG2 cells, and testify the protective effect of this extract against damage induced by the potent reactive oxygen species (ROS) inducer Paraquat (PQ), using the alkaline comet assay to measure basal DNA strand breaks (SBs) and to measure 8-oxoguanines and other altered purines (FPG sites) by incubation with formamidopyrimidine DNA glycosylase (FPG). PQ is an herbicide that produces ROS inside the cells and it has been appointed as a relevant factor in the development of Parkinson’s disease. In this study we analyzed its genotoxicity and it was intended to contribute to the establishment of an alternative protocol to the methods used for the induction of oxidative damage. The effect of different concentrations of PQ (1; 1.5; 2 and 5 μM)…

Advisors/Committee Members: Gaivão, Isabel O’Neill M., Leal, Fernanda.

Subjects/Keywords: Ginkgo biloba; Paraquato; Células HepG2; Ensaio de cometa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, S. C. M. d. (2013). Efeito do extracto de folhas de Ginkgo biloba em células HepG2 tratadas com Paraquato. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2825

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Sandra Cristina Machado da. “Efeito do extracto de folhas de Ginkgo biloba em células HepG2 tratadas com Paraquato.” 2013. Thesis, RCAAP. Accessed January 22, 2021. http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2825.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Sandra Cristina Machado da. “Efeito do extracto de folhas de Ginkgo biloba em células HepG2 tratadas com Paraquato.” 2013. Web. 22 Jan 2021.

Vancouver:

Silva SCMd. Efeito do extracto de folhas de Ginkgo biloba em células HepG2 tratadas com Paraquato. [Internet] [Thesis]. RCAAP; 2013. [cited 2021 Jan 22]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2825.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva SCMd. Efeito do extracto de folhas de Ginkgo biloba em células HepG2 tratadas com Paraquato. [Thesis]. RCAAP; 2013. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/2825

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

17. Hsu, Hui-lin. Evalution of Dengue virus RNA extraction methods and the study of viral-induced apoptosis of HepG2 hepatocyte.

Degree: Master, Biological Sciences, 2006, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904106-163202

► Dengue fever is an arthropod-borne transmit disease, caused by dengue virus.The principal vectors are Aedes aegypti and Aedes albopictus. Induce Dengue fever (DF) and a… (more)

▼ Dengue fever is an arthropod-borne transmit disease, caused by dengue virus.The principal vectors are Aedes aegypti and Aedes albopictus. Induce Dengue fever (DF) and a more severe form of dengue hemorrhagic fever (DHF), dengue shock syndrome (DSS). Dengue virus is the most prevalent arbovirus in tropical and subtropical regions. There is no specific drug and vaccine available for treatment and prevention. Therefore, DF is an important disease among transmit diseases in humans. For the effective control and prevention of DF transmission, rapid quantitative molecular biological methods are very important for the diagnosis of dengue fever. At present, there are many methods to isolate the RNA of Dengue virus; however, the new developed magnetic method has not been used for the Dengue virus isolation yet. At first, we evaluated various methods for Dengue virus isolation. The result indicate that the best method of RNA extraction for dengue virus is the QIAamp® Viral RNA kit manual extraction. There are no apparent differences of the effect for Dengue virus RNA isolation between filting film and magnetic bead method. Furthermore, DHF caused by Dengue virus is a very serious disease and the pathologic mechanism of DHF has not been elucidated completely. Both clinical and experimental trials have confirmed that the liver cell is one of the target infected by Dengue virus. And, the mechanism of Dengue virus-induced liver cell apoptosis remains poorly understood.Furthermore, there are free radical and cytokines production in patientï¼s serum in the acute phase of DF. Therefore, the role of antioxidant and p21 in the mechanisms should be elucidcited. Our preliminary data show that p21 mRNA expression increase in HepG2 after Dengue virus infection. NAC, GSH, and DPI all can attenuate Dengue-induced cell apoptosis. Howerer, the relationship between p21 expression and liver cell apoptosis should be further clarified in the near future. Advisors/Committee Members: Jong-kang Lin (committee member), Huei-sheng Huang (committee member), Jiin-tsuey Cheng (chair), Kung-chia Young (chair).

Subjects/Keywords: p21; HepG2 cell; antioxidants; Dengue

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hsu, H. (2006). Evalution of Dengue virus RNA extraction methods and the study of viral-induced apoptosis of HepG2 hepatocyte. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904106-163202

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hsu, Hui-lin. “Evalution of Dengue virus RNA extraction methods and the study of viral-induced apoptosis of HepG2 hepatocyte.” 2006. Thesis, NSYSU. Accessed January 22, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904106-163202.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hsu, Hui-lin. “Evalution of Dengue virus RNA extraction methods and the study of viral-induced apoptosis of HepG2 hepatocyte.” 2006. Web. 22 Jan 2021.

Vancouver:

Hsu H. Evalution of Dengue virus RNA extraction methods and the study of viral-induced apoptosis of HepG2 hepatocyte. [Internet] [Thesis]. NSYSU; 2006. [cited 2021 Jan 22]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904106-163202.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hsu H. Evalution of Dengue virus RNA extraction methods and the study of viral-induced apoptosis of HepG2 hepatocyte. [Thesis]. NSYSU; 2006. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904106-163202

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Dayse Caroline Severiano da Cunha. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers.

Degree: 2010, Universidade Federal do Rio Grande do Norte

URL: http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3752 ; http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3754

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A 140,0 kDa lectin was purified and characterized from the mushroom Clavaria cristata. The purification procedures from the crude extract of the mushroom comprised gel… (more)

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A 140,0 kDa lectin was purified and characterized from the mushroom Clavaria cristata. The purification procedures from the crude extract of the mushroom comprised gel filtration chromatography on Sephacryl s200 and ion exchange on Resource Q column. The purified lectin agglutinated all types of human erythrocytes with preference for trypsinized type O erythrocytes. The haemagglutinating activity is dependent of Ca 2+ ions and was strongly inhibited by the glycoprotein bovine submaxillary mucin (BSM) up to the concentration of 0, 125 mg/mL. The C. cristata lectin (CcL) was stable in the pH range of 2,5-11,5 and termostable up to 80 C. CcL molecular mass determined by gel filtration on a Superose 6 10 300 column was approximately 140,3 kDa. SDS polyacrilamide gel electrophoresis revealed a single band with a molecular mass of approximately 14,5 kDa, when the lectin was heated at 100 ⁰C in the presence or absence of β-mercaptoethanol. CcL induced activation of murine peritoneal macrophages in vitro resulting in the release of nitric oxide (NO), reaching the maximum production at 24 h. In experimental paw oedema model in mice, CcL showed proinflammatory activity being able to induce oedema formation. Cell viability of HepG2, MDA 435 e 3T3 cell lines was examined after 72 h of incubation with CcL in different concentrations (0,5-50 μg/mL). CcL inhibited HepG2 cells growth with an IC50 value of 50 μg/mL. In the present work, the observed immunomodulatory and antiproliferative effects indicate CcL as a possible immunomodulator compound, interfering in the macrophages immune response, taking possible anti-parasitic, anti-tumoral effects or diagnostic and/or therapeutic

Uma lectina de 140,0 kDa foi purificada e caracterizada a partir do extrato protéico do fungo Clavaria cristata. O processo de purificação a partir do extrato bruto do fungo compreendeu uma cromatografia de gel filtração SEPHACRYL S200 e uma cromatografia de troca iônica Resource Q em sistema FPLC-AKTA (Fast Protein Liquid Chromatography). A lectina de C. cristata (CcL) aglutinou todos os tipos de eritrócitos humanos com preferência pelos do tipo O tratados com tripsina. A atividade hemaglutinante de CcL se mostrou dependente do íon cálcio e foi fortemente inibida pela glicoproteína mucina bovina (BSM) até a concentração mínima de 0,125 mg/mL. CcL foi estável numa ampla faixa de pH, que variou entre 2,5-11,5 e termoestável até 80C por uma hora. A massa molecular da CcL, determ inada por cromatografia de gel filtração Superose 6 10 300 GL em sistema de FPLC-AKTA foi de aproximadamente 140,3 kDa e uma eletroforese SDS-PAGE revelou uma única banda com massa molecular de aproximadamente 14,5 kDa quando a lectina foi aquecida à temperatura de 100 ⁰C. CcL induziu a ativação de macrófagos murinos in vitro com conseqüente liberação de óxido nítrico atingindo a máxima produção de óxido nítrico no tempo de 24 h. Em modelo experimental de edema de pata em camundongos, a lectina do fungo apresentou atividade pró-inflamatória sendo capaz de…

Advisors/Committee Members: Elizeu Antunes dos Santos, João Paulo Matos Santos Lima.

Subjects/Keywords: BIOQUIMICA; Macrófagos; Óxido nítrico; HepG2; Edema de pata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cunha, D. C. S. d. (2010). Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers. (Thesis). Universidade Federal do Rio Grande do Norte. Retrieved from http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3752 ; http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cunha, Dayse Caroline Severiano da. “Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers.” 2010. Thesis, Universidade Federal do Rio Grande do Norte. Accessed January 22, 2021. http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3752 ; http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cunha, Dayse Caroline Severiano da. “Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers.” 2010. Web. 22 Jan 2021.

Vancouver:

Cunha DCSd. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers. [Internet] [Thesis]. Universidade Federal do Rio Grande do Norte; 2010. [cited 2021 Jan 22]. Available from: http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3752 ; http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cunha DCSd. Purificação, caracterização e efeitos imunomodulatório e antiproliferativo de uma lectina do fungo Clavaria cristata (Holmsk.) Pers. [Thesis]. Universidade Federal do Rio Grande do Norte; 2010. Available from: http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3752 ; http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=3754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. 財津, 有未. Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system : 脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する.

Degree: 博士(歯学), 2016, Nagasaki University / 長崎大学

URL: http://hdl.handle.net/10069/37090

► Recent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular… (more)

Subjects/Keywords: Porphyromonas gingivalis; Non-alcoholic fatty liver disease; Autophagy; Lysosome; HepG2 cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

財津, �. (2016). Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system : 脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/37090

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

財津, 有未. “Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system : 脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed January 22, 2021. http://hdl.handle.net/10069/37090.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

財津, 有未. “Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system : 脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する.” 2016. Web. 22 Jan 2021.

Vancouver:

財津 �. Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system : 脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10069/37090.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

財津 �. Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system : 脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/37090

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Nazemzadeh, Sima. Regulation of PPAR-?? target genes by GW501516 in HepG2 cells.

Degree: MS, Chemistry and Biochemistry, 2012, California State University – Northridge

URL: http://hdl.handle.net/10211.2/1879

► Peroxisome proliferator-activated receptors (PPARs) are nuclear receptor transcription factors that function as transcription factors regulating expression of various genes. The three isotopes known today are… (more)

▼ Peroxisome proliferator-activated receptors (PPARs) are nuclear receptor transcription factors that function as transcription factors regulating expression of various genes. The three isotopes known today are known as PPAR-??, PPAR-?? and PPAR-??. While PPAR-?? and PPAR-?? are involved in regulation of fatty acid ??-oxidation, adipocyte differentiation, and fat storage, PPAR-?? is also rapidly emerging as an important player in lipid metabolism. Recent research shows that PPAR-?? may play a central role in regulation of fatty acid oxidation in skeletal muscle and adipose tissue, and in regulating glucose output in the liver. It is activated by unsaturated or saturated long-chain fatty acids, by prostacyclin, by retinoic acid, and some eicosanoids. Pharmacological PPAR-?? agonist GW501516 has been synthesized and it activates PPAR-?? with a much higher selectivity compared to other PPAR isotypes. Administration of GW501516 has been shown to regulate fatty acid ??-oxidation in skeletal muscle and adipose tissues, and therefore proven to have a significant benefit in improving insulin sensitivity and reducing hyperglycemia in type II diabetes. In addition, PPAR-?? has been implicated in keratinocyte homeostasis and hepatic lipoprotein production. Several genes are relevant to lipid metabolism: ACC2, CPT1 and LPL. The enzyme carnitine palmitoyl transferase, CPTI, plays a major role in regulation of mitochondrial ??-oxidation and catalyzes the transport of long-chain fatty acids from the cytosol into the mitochondrial matrix in preparation for undergoing ??-oxidation. On the other hand, acetyl-CoA carboxylase, ACC2, also regulates the metabolism of fatty acids. When the enzyme is active, the product malonyl-CoA is produced and inhibits the transfer of the fatty acyl group from acyl-CoA and carnitine via CPTI, which inhibits ??-oxidation of fatty acids in the mitochondria. Lipoprotein lipase (LPL) is an enzyme that plays a key role in hydrolyzing triglycerides into free fatty acids in chylomicrons and very low density lipoproteins (VLDL) in order for entry to other tissues. PPAR-?? was activated with GW501516 in HepG2 cells and the gene transcripts and protein expression of ACC2, CPTI and LPL were quantified with end-point PCR, real-time Q-PCR and Western blot. The gene expression of CPTI was also analyzed in rat L6 skeletal muscle cells. It was observed that activation of PPAR-?? with an agonist reduces fatty acid synthesis and enhances genes involved in fatty acid oxidation in the liver. This therefore suggests a switch from a fat-storing pathway to a fat-burning pathway in the liver and a switch of fatty acid oxidation from skeletal muscle cells to liver cells for energy supply. Advisors/Committee Members: Medh, Jheem D. (advisor), Schrodi, Yann (committee member).

Subjects/Keywords: HepG2 cells; Dissertations, Academic – CSUN – Chemistry and Biochemistry – Biochemistry.

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nazemzadeh, S. (2012). Regulation of PPAR-?? target genes by GW501516 in HepG2 cells. (Masters Thesis). California State University – Northridge. Retrieved from http://hdl.handle.net/10211.2/1879

Chicago Manual of Style (16^th Edition):

Nazemzadeh, Sima. “Regulation of PPAR-?? target genes by GW501516 in HepG2 cells.” 2012. Masters Thesis, California State University – Northridge. Accessed January 22, 2021. http://hdl.handle.net/10211.2/1879.

MLA Handbook (7^th Edition):

Nazemzadeh, Sima. “Regulation of PPAR-?? target genes by GW501516 in HepG2 cells.” 2012. Web. 22 Jan 2021.

Vancouver:

Nazemzadeh S. Regulation of PPAR-?? target genes by GW501516 in HepG2 cells. [Internet] [Masters thesis]. California State University – Northridge; 2012. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10211.2/1879.

Council of Science Editors:

Nazemzadeh S. Regulation of PPAR-?? target genes by GW501516 in HepG2 cells. [Masters Thesis]. California State University – Northridge; 2012. Available from: http://hdl.handle.net/10211.2/1879

University of KwaZulu-Natal

21. Ghazi, Terisha. Fusaric acid-induced epigenetic modifications in vitro and in vivo: alternative mechanisms of hepatotoxicity.

Degree: 2019, University of KwaZulu-Natal

URL: https://researchspace.ukzn.ac.za/handle/10413/18611

► The Fusarium-produced mycotoxin, Fusaric acid (FA), is a frequent contaminant of agricultural foods that exhibits toxicity in plants and animals with little information on its… (more)

▼ The Fusarium-produced mycotoxin, Fusaric acid (FA), is a frequent contaminant of agricultural foods that exhibits toxicity in plants and animals with little information on its molecular and epigenetic mechanisms. Epigenetic modifications including DNA methylation, histone methylation, N-6-methyladenosine (m6A) RNA methylation, and microRNAs are central mediators of cellular function and may constitute novel mechanisms of FA toxicity. This study aimed to determine epigenetic mechanisms of FA-induced hepatotoxicity in vitro and in vivo by specifically investigating DNA methylation, histone 3 lysine (K) 9 trimethylation (H3K9me3), and m6A-mediated regulation of p53 expression in human liver (HepG2) cells and C57BL/6 mice livers. FA induced global DNA hypomethylation in HepG2 cells; decreased the expression of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) by inducing promoter hypermethylation and upregulated expression of miR-29b. Further, FA decreased the ubiquitination of DNMT1, DNMT3A, and DNMT3B by decreasing the expression of the ubiquitination regulators, UHRF1 and USP7. FA induced promoter hypomethylation of the demethylase, MBD2 and increased MBD2 expression contributing to global DNA hypomethylation in HepG2 cells. DNA methylation and H3K9me3 function in concert to regulate genome integrity and gene transcription. Sirtuin (Sirt) 1 is a histone deacetylase and direct target of miR-200a that regulates the repressive H3K9me3 mark by post-translationally modifying both H3K9Ac and the histone methyltransferase, SUV39H1. FA upregulated miR-200a and decreased Sirt1 expression in HepG2 cells and C57BL/6 mice livers. FA decreased the expression of SUV39H1 and histone demethylase, KDM4B which led to a decrease in H3K9me3 and an increase in H3K9me1. FA also decreased cell viability via apoptosis as evidenced by the significant increase in the activity of the executioner caspase-3/7. The tumor suppressor protein, p53 regulates cell cycle arrest and apoptosis in response to cellular stress. The expression of p53 is regulated at the transcriptional and post-transcriptional level by promoter methylation and m6A RNA methylation. In HepG2 cells, FA induced p53 promoter hypermethylation and decreased p53 expression. FA also decreased m6A-p53 levels by decreasing the expression of the methyltransferases, METTL3 and METTL14, and the m6A readers, YTHDF1, YTHDF3, and YTHDC2, thereby, decreasing p53 translation. In C57BL/6 mice livers FA, however, induced p53 promoter hypomethylation and increased p53 expression. FA increased m6A-p53 levels by increasing the expression of METTL3 and METTL14; and increased expression of YTHDF1, YTHDF3, and YTHDC2 increased p53 translation. In conclusion, this study provides evidence for alternative mechanisms of FA-induced hepatotoxicity (in vitro and in vivo) by modulating DNA methylation, H3K9me3, m6A RNA methylation, and epigenetically regulating p53 expression ultimately leading to genome instability and apoptotic cell death. These results provide insight into a better understanding of… Advisors/Committee Members: Chuturgoon, Anil Amichund. (advisor), Nagiah, Savania. (advisor).

Subjects/Keywords: Fusaric acid (FA).; Hepatotoxicity.; HepG2 cells.; DNA methylation.; Fusarium.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ghazi, T. (2019). Fusaric acid-induced epigenetic modifications in vitro and in vivo: alternative mechanisms of hepatotoxicity. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/18611

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ghazi, Terisha. “Fusaric acid-induced epigenetic modifications in vitro and in vivo: alternative mechanisms of hepatotoxicity.” 2019. Thesis, University of KwaZulu-Natal. Accessed January 22, 2021. https://researchspace.ukzn.ac.za/handle/10413/18611.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ghazi, Terisha. “Fusaric acid-induced epigenetic modifications in vitro and in vivo: alternative mechanisms of hepatotoxicity.” 2019. Web. 22 Jan 2021.

Vancouver:

Ghazi T. Fusaric acid-induced epigenetic modifications in vitro and in vivo: alternative mechanisms of hepatotoxicity. [Internet] [Thesis]. University of KwaZulu-Natal; 2019. [cited 2021 Jan 22]. Available from: https://researchspace.ukzn.ac.za/handle/10413/18611.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ghazi T. Fusaric acid-induced epigenetic modifications in vitro and in vivo: alternative mechanisms of hepatotoxicity. [Thesis]. University of KwaZulu-Natal; 2019. Available from: https://researchspace.ukzn.ac.za/handle/10413/18611

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Nova

22. Veiga, Kelly. Complexos de escorpionato: papel biológico como potenciais agentes anti-tumor.

Degree: 2011, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7034

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Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

O cancro é uma doença genética com origem em células somáticas desordenadas à… (more)

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Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

O cancro é uma doença genética com origem em células somáticas desordenadas à nível celular, que sofreram alterações permitindo escapar à vigilância do sistema imunitário. O tratamento por quimioterapia pretende controlar a disseminação da doença por metastização. Sendo assim, a investigação de compostos com acção citostática é de grande importância. Tendo sido comprovado a existência de complexos de escorpionato com essa actividade, pretendeu-se averiguar a acção citostática dos compostos TS199 e CoMeOH. Para este fim, procedeu-se a avaliação da citotoxicidade e da morte celular dos complexos de escorpionato em estudo nas linhas tumorais HCT116 e HepG2, recorrendo aos ensaios de MTS e de coloração pelo método de Hoechst. Procedeu-se também ao estudo dos alvos e modos de acção na levedura Saccharomyces cerevisiae, caracterizando a concentração mínima inibitória e a curva de crescimento, determinando a viabilidade celular e a actividade de espécies reactivas de oxigénio e estudando o seu proteoma. Os resultados obtidos para estes complexos foram comparados com os do antibiótico antracíclico, Doxorrubicina, uma vez que este composto tem acção citostática comprovada. Os resultados obtidos em linhas HCT116 e HepG2 permitiram inferir que os complexos TS199 e CoMeOH possuem actividade citostática e estimulam a via de morte celular por apoptose. Conclui-se também que, os compostos em estudo inibem o crescimento celular normal da levedura possuindo uma concentração mínima inibitória semelhante. No que diz respeito as espécies reactivas de oxigénio, não se observou a sua indução nem na presença do composto TS199 e nem do composto CoMeOH. Ambos os complexos induzem a inibição do metabolismo energético da levedura, nomeadamente as vias do metabolismo glicolítico e do piruvato. Por último, de entre os dois complexos de escorpionato o composto CoMeOH parece ter maior capacidade inibitória do que o composto TS199, tanto nas linhas tumorais como na levedura.

Advisors/Committee Members: Fernandes, Maria Alexandra, Martins, Marta.

Subjects/Keywords: Complexos de escorpionato; Acção citostática; HCT116; HepG2; Saccharomyces cerevisiae; Proteómica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Veiga, K. (2011). Complexos de escorpionato: papel biológico como potenciais agentes anti-tumor. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Veiga, Kelly. “Complexos de escorpionato: papel biológico como potenciais agentes anti-tumor.” 2011. Thesis, Universidade Nova. Accessed January 22, 2021. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Veiga, Kelly. “Complexos de escorpionato: papel biológico como potenciais agentes anti-tumor.” 2011. Web. 22 Jan 2021.

Vancouver:

Veiga K. Complexos de escorpionato: papel biológico como potenciais agentes anti-tumor. [Internet] [Thesis]. Universidade Nova; 2011. [cited 2021 Jan 22]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Veiga K. Complexos de escorpionato: papel biológico como potenciais agentes anti-tumor. [Thesis]. Universidade Nova; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/7034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Vienna

23. Bilek, Michael. Schutzwirkung von Safran auf H2O2-induzierte Zellschädigung in HepG2-Zellen.

Degree: 2010, University of Vienna

URL: http://othes.univie.ac.at/9107/

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Safran (crocus sativus L.) ist das teuerste Gewürz der Welt. Sein hoher Preis rührt von der Tatsache her, dass die Kultivierung von Hand durchgeführt wird.… (more)

▼

Safran (crocus sativus L.) ist das teuerste Gewürz der Welt. Sein hoher Preis rührt von der Tatsache her, dass die Kultivierung von Hand durchgeführt wird. Wegen seiner Inhaltsstoffe ist crocus sativus L. Gegenstand verschiedener wissenschaftlicher Untersuchungen. In der Vergangenheit wurde einzelnen Inhaltsstoffen antioxidatives Potential und sogar antikanzerogene Wirkungen nachgewiesen. Crocin und Safranal waren in diesem Zusammenhang im Fokus diverser Studien. Von einer Betrachtung isolierter Inhaltsstoffe wurde in dieser Arbeit abgesehen. In der hier durchgeführten in-vitro Studie wurden die zytotoxischen und genotoxischen Wirkungen von Narbenästen und Tepalen des Safrans auf Lebertumorzellen (HepG2-Zellen) getestet. Zusätzlich sollte eine potentielle Schutzwirkung des Safrans auf zuvor mit H2O2 inkubierten Zellen nachgewiesen werden. Zur Bestimmung der Zellvitalität wurde der Trypanblautest angewandt, zur Ermittlung der DNA-Schädigung wurde der Comet-Assay genutzt. Zusammenfassend konnte mit dieser Arbeit eine Tendenz einer erhöhten Zytotoxizität festgestellt werden. Vor allem bei höherer Konzentration von Narbenästen wurde dieses Resultat bestätigt. Sowohl bei den Narbenästen als auch bei den Tepalen kam es zu keiner signifikanten Schutzwirkung vor oxidativem Stress. Aufgrund dieser Beobachtungen lässt sich folgern, dass mittels des hier angewandten wissenschaftlichen Aufbaus dem Safran keine Schutzwirkung, in hoher Konzentration jedoch zytotoxische Tendenzen zugeschrieben werden können.

Saffron is the most expensive spice in the world, based on the harvesting condition which is still done by hand. Due to saffron’s ingredients it is focus of ongoing studies. In the past, research could prove that several ingredients exhibit antioxidative and even anticancerogenic properties. In this accomplished in vitro study, cytotoxic and genotoxic effects of saffron’s stigmas and tepals on liver-cancer-cells (HepG2) were compared. In addition, an assumed protective effect on H2O2 incubated cells was determined. The trypanblue-test was used in order to assign cell-vitality. The comet assay was used to prove DNA-damage. Especially at higher concentration of stigmas, a cytotoxic trend was assessed. Neither the stigmas nor the tepals have shown any protection from oxidative stress (H2O2 treated HepG2 cells) in the current study. Based on these observations, using the current scientific design, one cannot expect a protective effect of saffron. However, at high concentrations a cytotoxic tendency was noticed.

Subjects/Keywords: 30.99 Naturwissenschaften allgemein: Sonstiges; Safran / HepG2 / H2O2 / Zytotoxizität

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APA (6^th Edition):

Bilek, M. (2010). Schutzwirkung von Safran auf H2O2-induzierte Zellschädigung in HepG2-Zellen. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/9107/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bilek, Michael. “Schutzwirkung von Safran auf H2O2-induzierte Zellschädigung in HepG2-Zellen.” 2010. Thesis, University of Vienna. Accessed January 22, 2021. http://othes.univie.ac.at/9107/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bilek, Michael. “Schutzwirkung von Safran auf H2O2-induzierte Zellschädigung in HepG2-Zellen.” 2010. Web. 22 Jan 2021.

Vancouver:

Bilek M. Schutzwirkung von Safran auf H2O2-induzierte Zellschädigung in HepG2-Zellen. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Jan 22]. Available from: http://othes.univie.ac.at/9107/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bilek M. Schutzwirkung von Safran auf H2O2-induzierte Zellschädigung in HepG2-Zellen. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/9107/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

24. Foolchand, Ashmika. Methyl picolinic acid plays a role in epigenetic modifications in Human HepG2 liver cells.

Degree: 2019, University of KwaZulu-Natal

URL: https://researchspace.ukzn.ac.za/handle/10413/18610

► Picolinic Acid (PA) is an endogenous catabolite synthesized from L-Tryptophan via the kynurenine pathway. PA has been reported to possess immunological, neuroprotective, and anti-proliferative properties;… (more)

▼ Picolinic Acid (PA) is an endogenous catabolite synthesized from L-Tryptophan via the kynurenine pathway. PA has been reported to possess immunological, neuroprotective, and anti-proliferative properties; however, its most widely researched function is its efficient chelating properties. Despite these findings, the physiological function of PA is yet to be discovered. Many PA derivatives exist, with its methyl derivative being of interest in this study. Studies have shown that Picolinic Acid derivatives such as Fusaric Acid are involved in epigenetic regulation by inducing global DNA hypomethylation in HepG2 liver cells. Therefore, the aim of the study was to investigate the epigenetic properties of Picolinic Acid (parent molecule of Fusaric Acid) and Methyl Picolinic Acid in human HepG2 Liver cells. DNA methylation and Histone modifications are epigenetic phenomena that modify accessibility to DNA and chromatin structure, which results in regulation of gene expressions. While DNA methylation results in long-term repression, Histone methylation leads to formation of heterochromatin. The methyl-CpG binding domain 2 (MBD2) binds to methylated CpG dinucleotides and negatively regulates DNA methylation. MBD2 also associates with histone lysine methyltransferases, such as SUV39H1, which is involved in pericentric heterochromatin silencing and is responsible for methylating Histone 3 on lysine 9 (H3K9). The cytotoxicity of Picolinic Acid and Methyl Picolinic Acid in HepG2 liver cells was assessed by the WST-1 assay. The DNA methylation ELISA kit was used to quantify 5-methylcytosine in HepG2 cells. Gene expression for the DNA demethylase MBD2 was determined by qPCR. Protein expression for MBD2, trimethylated H3K9 (H3K9me3) and its methyltransferase, SUV39H1, was assessed by Western Blotting. The IC50 values for Picolinic Acid (7.5 mM) and Methyl Picolinic Acid (13 mM) were obtained from the WST-1 assay. DNA methylation was significantly decreased in Methyl Picolinic Acid, Picolinic Acid and 5-aza-2-DC treated cells (p<0.0001). In Methyl Picolinic Acid and Picolinic Acid treated cells, MBD2 gene expression was downregulated (p<0.0001) followed by an increase in its protein expression (p<0.0001). MPA increased protein expression of SUV39H1 (p<0.0001) leading to an increase in H3K9me3 (p<0.0001), while Picolinic Acid induced a decrease in SUV39H1 and H3K9me3 protein expression (p<0.0001). Methyl Picolinic Acid induced DNA hypomethylation as a result of increased MBD2 protein expression, despite the decrease in MBD2 gene expression. The upregulation of MBD2 protein expression promoted the activity of SUV39H1 which subsequently enhanced expression of H3K9me3. Since DNA hypomethylation can restore expression of aberrantly expressed genes, this study suggests a possible role for MPA in ameliorating carcinogenesis. Advisors/Committee Members: Chuturgoon, Anil Amichund. (advisor), Ghazi, Terisha. (advisor).

Subjects/Keywords: Methyl Picolinic Acid.; HepG2 liver cells.; L-Tryptophan.; DNA methylation.

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APA (6^th Edition):

Foolchand, A. (2019). Methyl picolinic acid plays a role in epigenetic modifications in Human HepG2 liver cells. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/18610

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Foolchand, Ashmika. “Methyl picolinic acid plays a role in epigenetic modifications in Human HepG2 liver cells.” 2019. Thesis, University of KwaZulu-Natal. Accessed January 22, 2021. https://researchspace.ukzn.ac.za/handle/10413/18610.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Foolchand, Ashmika. “Methyl picolinic acid plays a role in epigenetic modifications in Human HepG2 liver cells.” 2019. Web. 22 Jan 2021.

Vancouver:

Foolchand A. Methyl picolinic acid plays a role in epigenetic modifications in Human HepG2 liver cells. [Internet] [Thesis]. University of KwaZulu-Natal; 2019. [cited 2021 Jan 22]. Available from: https://researchspace.ukzn.ac.za/handle/10413/18610.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Foolchand A. Methyl picolinic acid plays a role in epigenetic modifications in Human HepG2 liver cells. [Thesis]. University of KwaZulu-Natal; 2019. Available from: https://researchspace.ukzn.ac.za/handle/10413/18610

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. C. Ricci. PCSK9 AND INFLAMMATION: IN VITRO STUDY ON HEPATOCYTES AND MACROPHAGES.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/541464

► TNF-alpha induces proprotein convertase subtilisin kexin type 9 (PCSK9) expression in hepatic HepG2 cell line, through the activation of suppressor of cytokine signaling 3 (SOCS3):… (more)

▼ TNF-alpha induces proprotein convertase subtilisin kexin type 9 (PCSK9) expression in hepatic HepG2 cell line, through the activation of suppressor of cytokine signaling 3 (SOCS3): Background. The suppressor of cytokine signaling (SOCS) proteins are negative regulators of the JAK/STAT pathway activated by pro-inflammatory cytokines, including the tumor necrosis factor- (TNF-). SOCS3 is also implicated in hypertriglyceridemia associated to insulin-resistance (IR). Proprotein Convertase Subtilisin Kexin Type 9 (PCSK9) levels are frequently found to be positively correlated to IR and plasma very low-density lipoprotein-triglycerides (VLDL-TG) concentrations. Aim. The present study aimed to investigate the possible role of TNF- and JAK/STAT pathway on de novo lipogenesis and PCSK9 expression in HepG2 cells. Methods and results. TNF- induced both SOCS3 and PCSK9 in a concentration-dependent manner. This effect was inhibited by transfection with siRNA anti-STAT3, suggesting the involvement of the JAK/STAT pathway. Retroviral overexpression of SOCS3 in HepG2 cells (HepG2SOCS3) strongly inhibited STAT3 phosphorylation and induced PCSK9 mRNA and protein levels, with no effect on its promoter activity. Consistently, siRNA anti-SOCS3 reduced PCSK9 mRNA levels while an opposite effect was observed with siRNA anti-STAT3. In addition, HepG2SOCS3 express higher mRNA levels of key enzymes involved in the de novo lipogenesis, such as fatty-acid synthase (FAS), stearoyl-CoA desaturase 1 (SCD-1), and apo-B. These responses were associated with significant increase of SCD-1 protein, activation of SREBP-1, accumulation of cellular TG and secretion of apoB. HepG2SOCS3 show lower phosphorylation levels of IRS-1 Tyr896 and Akt Ser473 in response to insulin. Finally, insulin stimulation produced an additive effect with SOCS3 overexpression, further inducing PCSK9, SREBP-1, FAS and apoB mRNA. Conclusions. Our data candidate PCSK9 as a gene involved in lipid metabolism regulated by pro-inflammatory cytokine TNF-, in a SOCS3 dependent manner. Proprotein subtilisin/kexin type 9 (PCSK9) induces pro-inflammatory response in macrophages: Background. Intraplaque release of inflammatory cytokines and chemokines from macrophages is directly implicated in atherogenesis, by inducing the proliferation and migration of media smooth muscle cells (SMCs) to the neointima. PCSK9 is present and released by SMCs within the atherosclerotic plaque but its role within the vascular wall is still unknown. Aim. In the present study, we tested the hypothesis of a pro-inflammatory effect of PCSK9 on macrophages. Methods and results. The pro-inflammatory effect of PCSK9 was assessed on THP-1-derived macrophages, exposed to different concentrations (0.250 ÷ 2.5 µg/ml) of human recombinant PCSK9 (hPCSK9). After exposure for 24h to 2.5 µg/ml PCSK9, a significant induction of IL-1β (8.17±2.88 fold), IL-6 (36.4±19.3 fold), TNF-α (67.4±25.9 fold), CXCL2 (42.6±0.0 fold), and MCP1 (17.0±6.8 fold), were observed. Importantly, physiological concentration of PCSK9… Advisors/Committee Members: supervisore: A. Corsini, coordinatore: A. L. Catapano, CATAPANO, ALBERICO LUIGI, CORSINI, ALBERTO.

Subjects/Keywords: inflammation; PCSK9; SOCS3; HepG2; macrophages; Settore BIO/14 - Farmacologia

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APA (6^th Edition):

Ricci, C. (2018). PCSK9 AND INFLAMMATION: IN VITRO STUDY ON HEPATOCYTES AND MACROPHAGES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/541464

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ricci, C.. “PCSK9 AND INFLAMMATION: IN VITRO STUDY ON HEPATOCYTES AND MACROPHAGES.” 2018. Thesis, Università degli Studi di Milano. Accessed January 22, 2021. http://hdl.handle.net/2434/541464.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ricci, C.. “PCSK9 AND INFLAMMATION: IN VITRO STUDY ON HEPATOCYTES AND MACROPHAGES.” 2018. Web. 22 Jan 2021.

Vancouver:

Ricci C. PCSK9 AND INFLAMMATION: IN VITRO STUDY ON HEPATOCYTES AND MACROPHAGES. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2434/541464.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ricci C. PCSK9 AND INFLAMMATION: IN VITRO STUDY ON HEPATOCYTES AND MACROPHAGES. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/541464

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

AUT University

26. Zou, Ying. Targeting MRP2 in HepG2 Cells Using the CRISPR-Cas9 System to Reverse Oxaliplatin Resistance .

Degree: AUT University

URL: http://hdl.handle.net/10292/12855

► As a third-generation platinum-based anticancer drug, oxaliplatin is critically important in the treatment of hepatocellular carcinoma (HCC), colorectal (CRC) and other GI cancers, improving disease-… (more)

▼ As a third-generation platinum-based anticancer drug, oxaliplatin is critically important in the treatment of hepatocellular carcinoma (HCC), colorectal (CRC) and other GI cancers, improving disease- and progression-free survival when added to combination chemotherapy regimens. However, due to the intrinsic and acquired drug resistance, its clinical efficacy is limited, which is still a challenging problem for successful chemotherapy of hepatocellular carcinoma. Previously studies indicated that membrane transporter MRP2-mediated drug efflux effects determine the cellular accumulation and cytotoxicity of platinum drugs. Therefore, we hypothesized that the resistance effect could be reversed by perturbation of ABCC2 gene (encoding MRP2). The CRISPR-Cas 9 system, as a gene editing tool, can be used to knock out the gene of interest. MRP2 by manipulating genes. We hypothesized that the CRISPR-Cas9 technology can be applied for knocking-out ABCC2 in an in vitro cell model overexpression MRP2 (i.e. HepG2 cell line). The HepG2 cells were transfected with ABCC2 guide-RNA/CAS9 protein ribonucleoprotein complexes through liposome-mediated delivery. The efficiency of ABCC2 gene disruption was then assessed using the T7 endonuclease I based method. The accumulation of a specific ABCC2 substrate 5(6)-carboxy-2',7'-dichlorofluorescein (CDCF) was determined in the gRNA/Cas9 transfected and wild type HepG2 cells by using flow cytometeric analysis. MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay was undertaken to determine oxaliplatin sensitivity, and concentration-dependent cytotoxic effects of oxaliplatin was compared between gRNA/Cas9 transfected and wild type HepG2 cells. Our current results reveal the feasibility of application of the liposome delivered CRISPR-Cas9 system to knock out ABCC2 gene in HepG2 cell line. Two sgRNAs were tested in this thesis, one of which showed that the target gene could not be effectively knocked out for unknown reasons. Fortunately, the second gRNA plays a role in disabling target genes with a genomic cleavage efficiency of 25% in HepG2 cells. Increased model MRP2 substrate (CDCF) accumulation and oxaliplatin cytotoxicity was observed for ABCC2-knockout HepG2 cells compared to control wild type. These results demonstrate that the on-target genomic editing in cell line treated by CRISPR-Cas9, and silencing ABCC2 gene by CRISPR-Cas9 does reduced the function of MRP2 protein. This thesis provide prove-of principle in vitro evidence supporting a novel therapeutic strategy that knockout of ABCC2 gene can decrease MRP2 function and increase oxaliplatin chemosensitivity in hepatocellular carcinoma. Advisors/Committee Members: Li, Yan (advisor).

Subjects/Keywords: MRP2; HepG2; CRISPR-Cas9; Oxaliplatin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zou, Y. (n.d.). Targeting MRP2 in HepG2 Cells Using the CRISPR-Cas9 System to Reverse Oxaliplatin Resistance . (Thesis). AUT University. Retrieved from http://hdl.handle.net/10292/12855

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zou, Ying. “Targeting MRP2 in HepG2 Cells Using the CRISPR-Cas9 System to Reverse Oxaliplatin Resistance .” Thesis, AUT University. Accessed January 22, 2021. http://hdl.handle.net/10292/12855.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zou, Ying. “Targeting MRP2 in HepG2 Cells Using the CRISPR-Cas9 System to Reverse Oxaliplatin Resistance .” Web. 22 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Zou Y. Targeting MRP2 in HepG2 Cells Using the CRISPR-Cas9 System to Reverse Oxaliplatin Resistance . [Internet] [Thesis]. AUT University; [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10292/12855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Zou Y. Targeting MRP2 in HepG2 Cells Using the CRISPR-Cas9 System to Reverse Oxaliplatin Resistance . [Thesis]. AUT University; Available from: http://hdl.handle.net/10292/12855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

27. Oskoei, Párástu Mariana Baghaei. Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools .

Degree: 2018, Universidade de Aveiro

URL: http://hdl.handle.net/10773/25436

► As acções antropogénicas são uma das principais fontes de contaminantes aquáticos presentes no meio ambiente, muitas vezes comprometendo o ecossistema e, consequentemente, os organismos presentes.… (more)

▼ As acções antropogénicas são uma das principais fontes de contaminantes aquáticos presentes no meio ambiente, muitas vezes comprometendo o ecossistema e, consequentemente, os organismos presentes. Os efeitos destes compostos na biota e nos seres humanos devem ser avaliados, adoptando metodologias de confiança. Os métodos comumente usados, como as abordagens in vivo, apresentam várias desvantagens. Além disso, a implementação da política dos 3R (Redução, Refinamento e Substituição) foi considerada uma prioridade, reforçando a necessidade de encontrar métodos alternativos. Tendo isto em conta, este trabalho teve como objectivos (i) validar a abordagem ex vivo, como uma alternativa na pesquisa animal, (ii) avaliar o potencial genotóxico de três pesticidas químicos, um insecticida (dimetoato), um fungicida (imazalil) e um herbicida (penoxsulam), e do bioinsecticida Turex® nas células de brânquias de lagostim (Procambarus clarkii), usando a abordagem ex vivo, e também (iii) determinar a citotoxicidade e genotoxicidade, in vitro, do Turex®, na linha celular HepG2. A viabilidade celular das células de brânquias e da linha celular HepG2 foi avaliada às 2, 4 e 8 horas e às 24 e 48 horas, respectivamente. A integridade do ADN foi avaliada usando o ensaio do cometa com a incubação usando enzimas de reparação específicas do DNA, nomeadamente a formamidopirimidina DNAglicosilase (FPG) e a endonuclease III (EndoIII), para avaliar a oxidação de purinas e pirimidinas, respectivamente. Relativamente à abordagem ex vivo, as células de brânquias de lagostim demonstraram ser adequadas apenas durante 2 horas, quando a viabilidade e a integridade do ADN foram consideradas em conjunto. Tendo em conta esta informação, as células das brânquias foram expostas durante 2 horas a concentrações ambientalmente realistas de inseticida dimetoato (20 μg L-1), do fungicida imazalil (160 μg L-1), e do herbicida penoxsulam (23 μg L-1). Adicionalmente, e relativamente à exposição ao bioinsecticida Turex®, duas abordagens distintas foram consideradas: (1) uma exposição ex vivo de células de brânquias do lagostim durante 2 horas a cinco concentrações (25, 50, 100, 200 e 400 μg L-1), onde a genotoxicidade foi avaliada usando o ensaio do cometa, e (2) uma exposição in vitro da linha celular HepG2 a outras cinco concentrações (250, 500, 1000, 1500 e 2000 μg L-1), durante 24 e 48 horas, onde a citotoxicidade e a genotoxicidade foram avaliadas, usando o teste MTT e o ensaio do cometa, respectivamente. O dimetoato, o imazalil e o penoxsulam demostraram ser genotóxicos para as células de brânquias de lagostim, apesar de não induzirem dano oxidativo no ADN. Por outro lado, o Turex® não foi capaz de exercer efeitos genotóxicos nas células de brânquias de lagostim, apesar de apresentar genotoxicidade na linha celular HepG2 (apesar de ser apenas sem activação do insecticida e após 48 h). Além disso, este biopesticida demonstrou ser citotóxico (principalmente quando activado e após 48 h) para a linha celular testada. Em… Advisors/Committee Members: Guilherme, Sofia Isabel Antunes Gomes (advisor), Oliveira, Helena Cristina Correia de (advisor).

Subjects/Keywords: Genotoxicidade; Pesticidas; Ex vivo; In vitro; Lagostim; HepG2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oskoei, P. M. B. (2018). Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools . (Thesis). Universidade de Aveiro. Retrieved from http://hdl.handle.net/10773/25436

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oskoei, Párástu Mariana Baghaei. “Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools .” 2018. Thesis, Universidade de Aveiro. Accessed January 22, 2021. http://hdl.handle.net/10773/25436.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oskoei, Párástu Mariana Baghaei. “Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools .” 2018. Web. 22 Jan 2021.

Vancouver:

Oskoei PMB. Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools . [Internet] [Thesis]. Universidade de Aveiro; 2018. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10773/25436.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oskoei PMB. Unravelling genotoxic effects of pesticides : in vitro and ex vivo assays as screening tools . [Thesis]. Universidade de Aveiro; 2018. Available from: http://hdl.handle.net/10773/25436

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. GRACE BIRUNGI. Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies.

Degree: 2010, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/22821

Subjects/Keywords: Microcystins; Capillary electrophoresis; Metabolomics; HepG2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

BIRUNGI, G. (2010). Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/22821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

BIRUNGI, GRACE. “Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies.” 2010. Thesis, National University of Singapore. Accessed January 22, 2021. http://scholarbank.nus.edu.sg/handle/10635/22821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

BIRUNGI, GRACE. “Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies.” 2010. Web. 22 Jan 2021.

Vancouver:

BIRUNGI G. Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies. [Internet] [Thesis]. National University of Singapore; 2010. [cited 2021 Jan 22]. Available from: http://scholarbank.nus.edu.sg/handle/10635/22821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

BIRUNGI G. Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies. [Thesis]. National University of Singapore; 2010. Available from: http://scholarbank.nus.edu.sg/handle/10635/22821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Notre Dame

29. Yilan Li. Botanical-Drug Interactions: Soy Isoflavones and Drug Metabolism</h1>.

Degree: Biological Sciences, 2005, University of Notre Dame

URL: https://curate.nd.edu/show/q237hq4081v

► Atherosclerosis is one of the leading causes of mortality and morbidity in western countries and high total cholesterol and high low-density lipoprotein (LDL) cholesterol… (more)

▼ Atherosclerosis is one of the leading causes of mortality and morbidity in western countries and high total cholesterol and high low-density lipoprotein (LDL) cholesterol levels are associated with the development of atherosclerosis. Soy intake has been documented to reduce the risk of heart disease by lowering blood cholesterol levels. Isoflavone-containing soyfood products and supplements are commercially available and promoted to treat several different health conditions including improving blood lipid profiles. The transport and metabolism of most xenobiotics are affected by the drug transporters and metabolizing enzymes in the liver and small intestine and the expression of these genes affect the potency of drugs or xenobiotics. Cytochrome P450s (CYPs) are important phase I drug-metabolizing enzymes in the liver and the expression levels of CYP1, 2, and 3 subfamilies are the rate-limiting factor for the turnover of many drugs. The cholesterol-lowering statins may be metabolized by different CYPs. Among the isoforms, CYP3A4 is responsible for nearly 54% of all drug-turnover and is considered most important. There is evidence showing that soy isoflavones may affect the expression of some of the genes in drug metabolism pathways. My project is focused on the effect of isoflavones on CYP1A2, 2C9, 3A4, as model isoforms of CYPs, and the identification of other genes pertaining to drug transport and biotransformation whose expression is affected by isoflavones. We have found that isoflavones upregulate CYP3A4 expression; and equol, a metabolite of daidzein, appears to be a most potent inducer of CYP3A4. Similarly, isoflavones also induce the expression of CYP1A2, but not CYP2C9. Using gene screening methods, two drug transporters, three phase I enzymes, and two phase II enzymes were identified to be induced by isoflavones in human hepatocytes exposed to isoflavones. CYP8B1, a gene encoding an enzyme at a branch point in the bile acid synthesis pathway appears to be an important target gene activated by isoflavones. Furthermore, an interaction between isoflavones and statins appears to affect CYP3A4 gene transcription and cellular lipid metabolism. Taken together, the isoflavones are able to affect the complex drug metabolism pathways and these in-vitro interactions of isoflavones with statins suggest clinical studies are warranted. Advisors/Committee Members: Michael Ferdig, Committee Member, Jo Ellen Welsh, Committee Member, Neil Shay, Committee Chair, Martin Tenniswood, Committee Member.

Subjects/Keywords: HepG2; Isoflavones; CYPs; Drug metabolism

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Y. (2005). Botanical-Drug Interactions: Soy Isoflavones and Drug Metabolism</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/q237hq4081v

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Yilan. “Botanical-Drug Interactions: Soy Isoflavones and Drug Metabolism</h1>.” 2005. Thesis, University of Notre Dame. Accessed January 22, 2021. https://curate.nd.edu/show/q237hq4081v.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Yilan. “Botanical-Drug Interactions: Soy Isoflavones and Drug Metabolism</h1>.” 2005. Web. 22 Jan 2021.

Vancouver:

Li Y. Botanical-Drug Interactions: Soy Isoflavones and Drug Metabolism</h1>. [Internet] [Thesis]. University of Notre Dame; 2005. [cited 2021 Jan 22]. Available from: https://curate.nd.edu/show/q237hq4081v.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li Y. Botanical-Drug Interactions: Soy Isoflavones and Drug Metabolism</h1>. [Thesis]. University of Notre Dame; 2005. Available from: https://curate.nd.edu/show/q237hq4081v

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Florida

30. Coyle, Jayme. The Assessment of an In-vitro Model for Evaluating the Role of PARP in Ethanol-mediated Hepatotoxicity.

Degree: 2013, University of South Florida

URL: https://scholarcommons.usf.edu/etd/4810

► This investigation assesses the role of poly(ADP-ribose) polymerase in ethanol-mediated hepatotoxicity using the untransfected HepG2 hepatocellular carcinoma line, an established, well-characterized toxicological model. HepG2 cells… (more)

Subjects/Keywords: Cytotoxicity; Ethanol Insensitivity; HepG2; Poly(ADP-Ribsoe) Polymerase; Toxicology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Coyle, J. (2013). The Assessment of an In-vitro Model for Evaluating the Role of PARP in Ethanol-mediated Hepatotoxicity. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/4810

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coyle, Jayme. “The Assessment of an In-vitro Model for Evaluating the Role of PARP in Ethanol-mediated Hepatotoxicity.” 2013. Thesis, University of South Florida. Accessed January 22, 2021. https://scholarcommons.usf.edu/etd/4810.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coyle, Jayme. “The Assessment of an In-vitro Model for Evaluating the Role of PARP in Ethanol-mediated Hepatotoxicity.” 2013. Web. 22 Jan 2021.

Vancouver:

Coyle J. The Assessment of an In-vitro Model for Evaluating the Role of PARP in Ethanol-mediated Hepatotoxicity. [Internet] [Thesis]. University of South Florida; 2013. [cited 2021 Jan 22]. Available from: https://scholarcommons.usf.edu/etd/4810.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coyle J. The Assessment of an In-vitro Model for Evaluating the Role of PARP in Ethanol-mediated Hepatotoxicity. [Thesis]. University of South Florida; 2013. Available from: https://scholarcommons.usf.edu/etd/4810

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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