Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(HMVEC). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Case Western Reserve University

1. Cheng, Xiwen. The Functional Study of Transcriptional Corepressor G-Protein Suppressor 2 (GPS2) and Tumor Suppressor Promyelocytic Leukemia (PML).

Degree: PhD, Biochemistry, 2010, Case Western Reserve University

We have identified G-protein pathway suppressor 2 (GPS2) as an integral component of the silencing mediator of retinoid acid and thyroid hormone receptor (SMRT) corepressor complex. Functional studies revealed that GPS2 is functionally important for estrogen receptor alpha (ERa)-mediated transcriptional regulation, and that GPS2/SMRT complex is important for maintaining normal proliferation of MCF-7 breast cancer cells. The promyelocytic leukemia protein (PML) is a well-known tumor suppressor, but its role in endothelial cells (ECs) is largely unknown, despite its high expression in endothelium and inflamed tissues. We have demonstrated that PML negatively regulates angiogenesis and cell migration, reconciling with upstream signal transducer and activator of transcription 1 (STAT1) and downstream integrin ß1 (ITGB1), thus identifying a novel pathway in which PML mediates the TNFa/IFNa signaling in ECs. Finally, we carried out microarray analysis in ECs and identified the differentially expressed genes when PML is knocked down and/or when cells are treated with TNFa. We further characterized the functional ontology of these gene lists and identified groups of interesting genes that warrant future study. Advisors/Committee Members: Samols, David (Committee Chair), Kao, Hung-Ying (Advisor).

Subjects/Keywords: Biochemistry; Bioinformatics; Cellular Biology; Molecular Biology; GPS2; SMRT; Estrogen Receptor alpha; ER&945; pS2; MCF-7; ChIP; proliferation; tamoxifen resistance; PML; angiogenesis; migration; HUVEC; HMVEC; cytokine; TNF&945; IFN&945; ITGB1

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cheng, X. (2010). The Functional Study of Transcriptional Corepressor G-Protein Suppressor 2 (GPS2) and Tumor Suppressor Promyelocytic Leukemia (PML). (Doctoral Dissertation). Case Western Reserve University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1277741995

Chicago Manual of Style (16th Edition):

Cheng, Xiwen. “The Functional Study of Transcriptional Corepressor G-Protein Suppressor 2 (GPS2) and Tumor Suppressor Promyelocytic Leukemia (PML).” 2010. Doctoral Dissertation, Case Western Reserve University. Accessed November 19, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1277741995.

MLA Handbook (7th Edition):

Cheng, Xiwen. “The Functional Study of Transcriptional Corepressor G-Protein Suppressor 2 (GPS2) and Tumor Suppressor Promyelocytic Leukemia (PML).” 2010. Web. 19 Nov 2019.

Vancouver:

Cheng X. The Functional Study of Transcriptional Corepressor G-Protein Suppressor 2 (GPS2) and Tumor Suppressor Promyelocytic Leukemia (PML). [Internet] [Doctoral dissertation]. Case Western Reserve University; 2010. [cited 2019 Nov 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1277741995.

Council of Science Editors:

Cheng X. The Functional Study of Transcriptional Corepressor G-Protein Suppressor 2 (GPS2) and Tumor Suppressor Promyelocytic Leukemia (PML). [Doctoral Dissertation]. Case Western Reserve University; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1277741995

2. Barnett, Petrina K. EPAS1 Promotes Endothelial Cell Migration in an IRX3-Dependent Manner.

Degree: PhD, Biological Science, 2016, Clark University Atlanta

Iroquois homeobox gene 3 (Irx3) is a transcription factor belonging to the Iroquois family of homeobox genes that is expressed in the embryonic organs of multiple organisms. Although much is known about Irx3 during embryogenesis, the cross talk of expression and function of Irx3 in peripheral vascular disease (PVD) remains to be investigated. Herein, we demonstrate that Endothelial PAS Domain 1 (EPAS1), which is involved in vasculogenesis and diseases associated with hypoxia, is an upstream regulator of Irx3, and this interaction contributes to the cellular movement of human microvascular endothelial cells (HMVECs). Genetic EPAS1 loss-of-function (LOF) studies in HMVECs resulted in a reduction of Irx3 mRNA, and Irx3-mediated endothelial cell migration in wound healing. In contrast, the effects of EPAS1 Gain-of-function (GOF) in HMVECs showed increased Irx3-mediated endothelial cell migration in wound healing. Taken together, these results reveal that Irx3 is an important regulator of cellular movement as a downstream target of EPAS1 signaling in HMVECs, which regulates migration. Advisors/Committee Members: Valerie Odero-Marah, PhD, Leonard M. Anderson, PhD, Cimona V. Hinton, PhD.

Subjects/Keywords: Irx3; EPAS1; Angiogenesis; Cell Migration; HMVEC; Tie2; Biochemistry, Biophysics, and Structural Biology; Biology; Cancer Biology; Cell and Developmental Biology; Diseases; Medicine and Health Sciences; Molecular Biology; Other Cell and Developmental Biology

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Barnett, P. K. (2016). EPAS1 Promotes Endothelial Cell Migration in an IRX3-Dependent Manner. (Doctoral Dissertation). Clark University Atlanta. Retrieved from http://digitalcommons.auctr.edu/cauetds/29

Chicago Manual of Style (16th Edition):

Barnett, Petrina K. “EPAS1 Promotes Endothelial Cell Migration in an IRX3-Dependent Manner.” 2016. Doctoral Dissertation, Clark University Atlanta. Accessed November 19, 2019. http://digitalcommons.auctr.edu/cauetds/29.

MLA Handbook (7th Edition):

Barnett, Petrina K. “EPAS1 Promotes Endothelial Cell Migration in an IRX3-Dependent Manner.” 2016. Web. 19 Nov 2019.

Vancouver:

Barnett PK. EPAS1 Promotes Endothelial Cell Migration in an IRX3-Dependent Manner. [Internet] [Doctoral dissertation]. Clark University Atlanta; 2016. [cited 2019 Nov 19]. Available from: http://digitalcommons.auctr.edu/cauetds/29.

Council of Science Editors:

Barnett PK. EPAS1 Promotes Endothelial Cell Migration in an IRX3-Dependent Manner. [Doctoral Dissertation]. Clark University Atlanta; 2016. Available from: http://digitalcommons.auctr.edu/cauetds/29

.