subject:(Gordonia Bacteria )

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Universidade Estadual de Campinas

1. Domingos, Daniela Ferreira, 1984-. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Abstract: Mangrove is an environment rich in microbial diversity, but little has been reported about it in Brazil, making the knowledge and exploitation of new microorganisms in mangroves an imperative issue. The prospection of microbial diversity in underexplored environments, such as mangroves, increses possibility of success in looking for new bioactive molecules, contributing to a more sustentable economy and environment. Although the cultivation techniques have been improved, allowing an increased number of yet uncultivated microorganisms to be able recuperated in vitro, our knowledge about their ecology is still insufficient to cultivate the majority of them. Therefore, the metagenomic library have emerged as a powerful tool to more widely access the overall microbial diversity in an environment, enabling the functional analyses of genes and the discovery of new bioactive compounds, mainly uncultured-microorganisms. The aim of the current work was the prospection of biosurfactant from mangrove microbiota through a polifasica approach. For the independent-cultivation approach, a metagenomic library of high molecular weight was constructed from mangrove sediment contaminated with oil. The clones were subjected to functional and molecular screening to identify biosurfactant activity. Three clones with the potential to procuce biosurfactant were selected, and the fosmid sequencing for genetic chatacterization of the insert was carried out. Although the clones showed the ability to reduce the surface tension, genes that may involved in biosurfactant synthesis were not identified. The results showed that using the functional metagenomic to explore metabolic pathways of microbial communities can have great limitation when in the prospection of biosurctants when the operon are larger then 30-40 kb and the genome of wild bacteria contains sparse regulation elements. For the dependent-cultivation approach, two strains that produce biosurfactant were selected: Bacillus safensis CCMA-560 and Gordonia sp. CCMA-559. They were isolated and tested in previous study. The biosurfactant production was opmized through the Placktt-Burman design and the Central Composite Design. The pumilacidin produced by B. safensis CCMA-560 was chemically characterized, and the metabolic pathway that is responsible for its production was genetically characterized. This work was the first report the physiologic, genetic, and chemical analysis on the biosurfactant production by the B. safensis strain Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Melo, Itamar Soares de (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Andreote, Fernando Dini (committee member), Araújo, Welington Luiz de (committee member), Vicentini, Renato (committee member), Duarte, Marta Cristina Teixeira (committee member).

Subjects/Keywords: Biossurfactantes; Metagenômica; Bacillus safensis; Gordonia (Bactéria); Biosurfactants; Metagenomics; Bacillus safensis; Gordonia (Bacteria)

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APA (6^th Edition):

Domingos, Daniela Ferreira, 1. (2014). Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Domingos, Daniela Ferreira, 1984-. “Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.” 2014. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Domingos, Daniela Ferreira 1. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Domingos, Daniela Ferreira 1. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Washington

2. Garg, Aparna. The effect of different sludge pretreatment methods on mitigation of anaerobic digester foaming.

Degree: 2017, University of Washington

URL: http://hdl.handle.net/1773/40528

In this study, waste activated sludge obtained from two full-scale treatment plants with foaming issues was pretreated with acid/alkali treatment, acid-phase fermentation, thermal treatment, sonication, and metal salt treatment to investigate their effect on foam mitigation. The major foam forming organisms were identified by microscopy and qPCR as Gordonia amarae, which are characterized as branched filaments. Waste activated sludge was characterized for foaming index prior to each pretreatment method. After carrying out different pretreatment methods, the foaming potential was again measured in batch experiments carried at different holding times per pretreatment methods. Among all pretreatment methods investigated, acid/alkali treatment and acid phase fermentation both showed a high reduction in foaming and in inactivation of G. amarae filaments and thermal treatment combined at alkaline pH enhanced the effectiveness of foam reduction. The maximum foaming reduction of 56% was achieved upon holding the sample for 2.5 h at 70 °C and pH of 11. Pretreatment methods that resulted in a sharp decrease in the foaming potential concurred with numbers in dead foam formers as confirmed using live/dead staining and the PMA-qPCR technique. Pretreating with FeCl3 gave good foaming reductions with 53.5% reduction in the unstable foaming potential at a concentration of 260g/L but did not result in a decrease of foam formers as confirmed by live/dead staining. Sonication did not prove effective in lowering the foaming index or killing the G.amarae filaments. Advisors/Committee Members: Winkler, Mari (advisor).

Subjects/Keywords: Anaerobic digester foaming; Filamentous bacteria; Gordonia amarae; live/dead stain; PMA-qPCR; Pretreatment; Environmental engineering; Civil engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Garg, A. (2017). The effect of different sludge pretreatment methods on mitigation of anaerobic digester foaming. (Thesis). University of Washington. Retrieved from http://hdl.handle.net/1773/40528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Garg, Aparna. “The effect of different sludge pretreatment methods on mitigation of anaerobic digester foaming.” 2017. Thesis, University of Washington. Accessed January 16, 2021. http://hdl.handle.net/1773/40528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Garg, Aparna. “The effect of different sludge pretreatment methods on mitigation of anaerobic digester foaming.” 2017. Web. 16 Jan 2021.

Vancouver:

Garg A. The effect of different sludge pretreatment methods on mitigation of anaerobic digester foaming. [Internet] [Thesis]. University of Washington; 2017. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1773/40528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Garg A. The effect of different sludge pretreatment methods on mitigation of anaerobic digester foaming. [Thesis]. University of Washington; 2017. Available from: http://hdl.handle.net/1773/40528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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