subject:(GnRH)

1. Vosges, Mélanie. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.

Degree: Docteur es, Sciences de la vie, 2010, Université François-Rabelais de Tours

URL: http://www.theses.fr/2010TOUR4035

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A ce jour, les effets des perturbateurs endocriniens (PE) sur les circuits neuroendocrines contrôlant la fonction de reproduction ont fait l’objet de très peu de… (more)

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A ce jour, les effets des perturbateurs endocriniens (PE) sur les circuits neuroendocrines contrôlant la fonction de reproduction ont fait l’objet de très peu de travaux. Chez les vertébrés, l’élément majeur du contrôle central de la fonction de reproduction est la GnRH (Gonadotropin-Releasing Hormone). Le développement et l’activité des neurones à GnRH sont finement régulés, notamment par les hormones stéroïdes, ce qui les rend potentiellement sensibles aux PE. L’objectif de ce travail était d'étudier les effets neuroendocrines des xéno-œstrogènes chez le poisson zèbre (Danio rerio). Nous montrons que le 17α-éthinylestradiol (EE2) et le nonylphénol (NP) perturbent l'ontogenèse du système à GnRH au cours du développement précoce. De plus, nous démontrons que ces effets impliquent des récepteurs des œstrogènes. Parallèlement, nous mettons en évidence l’effet inducteur de l’EE2 et du NP sur l'expression de l’aromatase cérébrale, l’enzyme de synthèse des œstrogènes. L’ensemble de ces données souligne la nécessité de considérer les réseaux neuroendocrines comme des variables critiques et sensibles dans le domaine de la perturbation endocrinienne.

Until now, studies dedicated to the actions of endocrine disrupting chemicals (EDCs) on the reproductive axis have focused on the gonads and peripheral organs leaving virtually unexplored their actions on neuroendocrine circuits controlling reproduction. In vertebrates, gonadotropin-releasing hormone (GnRH) is the key factor controlling the activity of the reproductive axis. The development and functioning of GnRH neurons are finely tuned, notably by sex steroids, making these neurons potential targets of EDCs. The aim of this work was to explore the neuroendocrine effects of xenoestrogens in the zebrafish (Danio rerio). We show that 17α-ethinylestradiol (EE2) and nonylphenol (NP) disrupts the ontogeny of GnRH system during zebrafish early life stage. Moreover, we demonstrate that these effects involve functional estrogens receptors. In parallel, we report the inducing effects of EE2 and NP on the expression of brain aromatase protein, the enzyme responsible for estrogen biosynthesis. Altogether, these results highlight the need to consider neuroendocrine networks as critical and sensitive endpoints in the field of endocrine disruption.

Advisors/Committee Members: Combarnous, Yves (thesis director).

Subjects/Keywords: GnRH

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APA (6^th Edition):

Vosges, M. (2010). Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2010TOUR4035

Chicago Manual of Style (16^th Edition):

Vosges, Mélanie. “Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.” 2010. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 22, 2019. http://www.theses.fr/2010TOUR4035.

MLA Handbook (7^th Edition):

Vosges, Mélanie. “Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.” 2010. Web. 22 Mar 2019.

Vancouver:

Vosges M. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2010. [cited 2019 Mar 22]. Available from: http://www.theses.fr/2010TOUR4035.

Council of Science Editors:

Vosges M. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2010. Available from: http://www.theses.fr/2010TOUR4035

University of Otago

2. Cheong, Rachel Yanghui. Effects of estradiol on gonadotropin-releasing hormone neurons .

Degree: 2012, University of Otago

URL: http://hdl.handle.net/10523/2570

► The gonadotropin-releasing hormone (GnRH) neurons, located within the hypothalamus of the mammalian brain, have been established as the key output cells mediating all influences that… (more)

▼ The gonadotropin-releasing hormone (GnRH) neurons, located within the hypothalamus of the mammalian brain, have been established as the key output cells mediating all influences that control fertility, conveyed by the central nervous system. Gonadotropins (luteinizing hormone; LH and follicle stimulating hormone; FSH), secreted by the pituitary in response to hypothalamic signals from GnRH neurons, stimulate the release of gonadal steroids from the ovaries. The gonadal steroid, estradiol (E2), functions as a diverse and indispensible hormone capable of modulating multiple facets of neuronal functioning. Circulating E2 provides neuroendocrine feedback to the hypothalamus and pituitary, through negative and positive feedback mechanisms depending on the hormonal milieu across the ovulatory cycle. E2 exerts a negative feedback response on GnRH neurons to keep basal gonadotropin secretion low and switches over to positive feedback to generate the pre-ovulatory gonadotropin surge, required for ovulation. Although GnRH neurons express estrogen receptor β (ERβ), the other receptor subtype, ERα, has been described as the main receptor mediating positive feedback. However, a role for both ERα and ERβ in estrogen negative feedback remains likely. The mechanisms underlying negative feedback actions of estrogen upon the GnRH neuronal network are unclear and likely to require different mechanisms of E2 action including direct actions through ERβ-expressing GnRH neurons or indirectly through ERα-expressing afferent inputs to GnRH neurons. E2 is known to act through the non-classical pathway to rapidly activate intracellular signaling cascades to alter cellular function and it is believed that these rapid non-classical E2 actions are likely contributors involved in mediating estrogen negative feedback in mice. The purpose of this study was to investigate the mechanisms mediating estrogen negative feedback on the GnRH neuronal system. The specific intracellular signaling pathways mediating rapid non-classical E2 actions were first addressed employing both in vivo and in vitro acute brain slice approaches involving dual-label fluorescence and peroxidase-based immunohistochemistry in a negative feedback model. This model involved ovariectomizing mice and testing the ability of the acute E2 treatment to rapidly induce the phosphorylation of the cyclic-AMP response element binding protein (CREB) and the upstream molecule, extracellular signal-regulated kinase 1 and 2 (ERK1/2). The administration of E2 to adult female mice in vivo resulted in the rapid activation of ERK1/2 in GnRH neurons in a time-dependent manner and in vitro studies using pharmacological antagonists showed that ERK1/2 was essential for E2-induced CREB phosphorylation in GnRH neurons. Upstream to this, protein kinase A (PKA) and calcium/calmodulin dependent protein kinase type II (CaMKII), but not protein kinase C were found to be necessary for E2-induced phosphorylation of ERK1/2 and CREB. Interestingly, this rapid E2 signaling cascade in GnRH neurons was found to… Advisors/Committee Members: Ábrahám, István (advisor).

Subjects/Keywords: Estradiol; GnRH; negativefeedback; non-classical

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APA (6^th Edition):

Cheong, R. Y. (2012). Effects of estradiol on gonadotropin-releasing hormone neurons . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/2570

Chicago Manual of Style (16^th Edition):

Cheong, Rachel Yanghui. “Effects of estradiol on gonadotropin-releasing hormone neurons .” 2012. Doctoral Dissertation, University of Otago. Accessed March 22, 2019. http://hdl.handle.net/10523/2570.

MLA Handbook (7^th Edition):

Cheong, Rachel Yanghui. “Effects of estradiol on gonadotropin-releasing hormone neurons .” 2012. Web. 22 Mar 2019.

Vancouver:

Cheong RY. Effects of estradiol on gonadotropin-releasing hormone neurons . [Internet] [Doctoral dissertation]. University of Otago; 2012. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10523/2570.

Council of Science Editors:

Cheong RY. Effects of estradiol on gonadotropin-releasing hormone neurons . [Doctoral Dissertation]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2570

University of Virginia

3. Roland, Alison Victoria. Metabolic Regulation of Fertility: An Emerging Role for AMP-activated Protein Kinase in the Control of Gonadotropin-releasing Hormone Neuron Function.

Degree: PhD, Pharmacology, 2014, University of Virginia

URL: http://libra.virginia.edu/catalog/libra-oa:5576

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Gonadotropin-releasing hormone (GnRH) neurons integrate steroidal and metabolic cues to regulate fertility centrally. Abnormalities in GnRH release play a role in polycystic ovary syndrome (PCOS),… (more)

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Gonadotropin-releasing hormone (GnRH) neurons integrate steroidal and metabolic cues to regulate fertility centrally. Abnormalities in GnRH release play a role in polycystic ovary syndrome (PCOS), the leading cause of infertility in women. PCOS is characterized by elevated androgen and luteinizing hormone (LH) levels, oligoor anovulation, and/or ovarian cysts. Increased central stimulation of the reproductive axis due to increased frequency and amplitude of GnRH secretion is thought to play an important pathophysiological role in this disorder. Metabolic abnormalities, including insulin resistance, are also extremely common in PCOS. Because glucose homeostasis is perturbed in PCOS, we examined metabolic characteristics in female mice prenatally androgenized (PNA) with dihydrotestosterone. PNA mice exhibit increased central drive to the reproductive axis and irregular reproductive cycles, similar to PCOS. PNA mice had impaired glucose tolerance, but did not exhibit peripheral insulin resistance and hyperinsulinemia, suggesting that excess prenatal androgen receptor activation may be insufficient to cause the full spectrum of metabolic abnormalities seen in PCOS. Evidence indicates that glucose is critical in regulating reproduction centrally, but the cellular mechanisms are poorly understood. We examined if changes in extracellular glucose could alter the activity of GnRH neurons, and if glucosensing was affected by dihydrotestosterone and/or estradiol. GnRH neurons were sensitive to physiological reductions in extracellular glucose; androgens inhibited glucosensing. Mechanistically, AMPK proved to be important mediator of glucosensing, and AMPK activation inhibited GnRH neurons. ii The inhibitory effect of AMPK activation led us to hypothesize that metformin, an AMPK activator that restores reproductive function in PCOS, might blunt excessive GnRH release through direct central actions. We tested this hypothesis in PNA mice. Metformin restored estrous cyclicity to PNA mice and reduced LH and GnRH neuronal activity. GnRH neurons from metformin-treated mice were stimulated by an AMPK antagonist and relatively insensitive to low glucose, suggesting AMPK was activated centrally by metformin. Together, these studies have provided novel insight into the regulation of GnRH neuronal activity by metabolic and steroidal cues, shown how interactions between these cues might contribute to the PCOS phenotype, and elucidated the mechanisms by which metformin improves reproductive function in women with PCOS.

Note: Abstract extracted from PDF text

Advisors/Committee Members: Keller, Susanna (advisor), Shupnik, Margaret (advisor).

Subjects/Keywords: metabolic regulation; fertility; GnRH

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APA (6^th Edition):

Roland, A. V. (2014). Metabolic Regulation of Fertility: An Emerging Role for AMP-activated Protein Kinase in the Control of Gonadotropin-releasing Hormone Neuron Function. (Doctoral Dissertation). University of Virginia. Retrieved from http://libra.virginia.edu/catalog/libra-oa:5576

Chicago Manual of Style (16^th Edition):

Roland, Alison Victoria. “Metabolic Regulation of Fertility: An Emerging Role for AMP-activated Protein Kinase in the Control of Gonadotropin-releasing Hormone Neuron Function.” 2014. Doctoral Dissertation, University of Virginia. Accessed March 22, 2019. http://libra.virginia.edu/catalog/libra-oa:5576.

MLA Handbook (7^th Edition):

Roland, Alison Victoria. “Metabolic Regulation of Fertility: An Emerging Role for AMP-activated Protein Kinase in the Control of Gonadotropin-releasing Hormone Neuron Function.” 2014. Web. 22 Mar 2019.

Vancouver:

Roland AV. Metabolic Regulation of Fertility: An Emerging Role for AMP-activated Protein Kinase in the Control of Gonadotropin-releasing Hormone Neuron Function. [Internet] [Doctoral dissertation]. University of Virginia; 2014. [cited 2019 Mar 22]. Available from: http://libra.virginia.edu/catalog/libra-oa:5576.

Council of Science Editors:

Roland AV. Metabolic Regulation of Fertility: An Emerging Role for AMP-activated Protein Kinase in the Control of Gonadotropin-releasing Hormone Neuron Function. [Doctoral Dissertation]. University of Virginia; 2014. Available from: http://libra.virginia.edu/catalog/libra-oa:5576

Universiteit Utrecht

4. Athmer, P.L.T.M. Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana).

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/186901

► Elephant populations in Southern Africa have been increasing in number following their protective status as endangered species by CITES in 1989, to the extent that… (more)

▼ Elephant populations in Southern Africa have been increasing in number following their protective status as endangered species by CITES in 1989, to the extent that elephant populations have to be regulated. Elephant population control can be done in two basic ways: decreasing their number (i.e. culling or relocation) or preventing further increase by reduction of their reproductive rate (i.e. contraception). Culling elephants is an effective but controversial method due to public disapproval, while translocation is a less suitable method due to various practical problems. Contraceptive methods should ideally meet certain prerequisites based on efficacy, minimal animal handling, reversibility, safety for use in pregnant animals, minimal health side effects, no passage through the food chain, minimal effects on behaviour and low costs. Contraceptive methods as surgical sterilization, mechanical and hormonal contraception do not meet all of these prerequisites. Immunocontraception has become a point of interest with the antigens zona pellucida (ZP) and gonadotrophin-releasing hormone (GnRH) most intensively studied. GnRH is a neuropeptide produced by the hypothalamus and plays a crucial role in hypothalamic-pituitary-ovarian axis involved in ovulation. By vaccinating against GnRH, antibodies are thought to bind to GnRH, thereby blocking an important step in the ovulation process. The ultimate purpose of GnRH vaccination is to induce infertility by returning treated animals to a (temporary) pharmalogical pre-pubertal state. The eventual aim of this study was to test a commercial GnRH vaccine, Improvac®, for its effect on female African elephants. In this current pilot study we measured the progestagen and glucocorticoids metabolite levels in the faeces of two African elephant cows during an entire oestrus cycle (20 weeks) prior to GnRH vaccination. The faecal concentrations were estimated using Enzyme Immuno Assays (EIA) for 5α pregnane-3-ol-20-one (progesterone metabolite) and 3α,11-Oxo-cortisol (glucocorticoid metabolite). The aim by doing so was to provide a baseline for faecal progestagen and glucocorticoid concentrations in cycling non-pregnant African elephant cows and investigate correlations between reproductive status (progestagen) and stress (glucocorticoids) using a non-invasive sampling method. - 3 - Although the number of samples analysed in this study was limited, it did demonstrate that faecal progesterone concentrations could be used to demonstrate luteal activity, making it of considerable use for assessing the effects of GnRH vaccination. Faecal glucocorticoids levels did not appear to be significantly influenced by stage of cycle. A complete database of glucocorticoids levels during the long term studies could be helpful to determine whether GnRH vaccination is detrimental to welfare. Advisors/Committee Members: Stout, Prof TAE.

Subjects/Keywords: Diergeneeskunde; elephant, GnRH, progesterone, glucocorticoid

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APA (6^th Edition):

Athmer, P. L. T. M. (2008). Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana). (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/186901

Chicago Manual of Style (16^th Edition):

Athmer, P L T M. “Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana).” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed March 22, 2019. http://dspace.library.uu.nl:8080/handle/1874/186901.

MLA Handbook (7^th Edition):

Athmer, P L T M. “Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana).” 2008. Web. 22 Mar 2019.

Vancouver:

Athmer PLTM. Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana). [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2019 Mar 22]. Available from: http://dspace.library.uu.nl:8080/handle/1874/186901.

Council of Science Editors:

Athmer PLTM. Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana). [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/186901

Louisiana State University

5. Johnson, Carrie Ann. Endocrine and reproductive responses to implants of deslorein acetate in horses.

Degree: PhD, Animal Sciences, 2002, Louisiana State University

URL: etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218

► Four experiments were performed to study the effects of the gonadotropin releasing hormone (GnRH) analog, deslorelin acetate (Ovuplant™), on endocrine and reproductive characteristics in mares.… (more)

▼ Four experiments were performed to study the effects of the gonadotropin releasing hormone (GnRH) analog, deslorelin acetate (Ovuplant™), on endocrine and reproductive characteristics in mares. The first experiment tested whether anecdotal field reports of Ovuplant causing extended interovulatory intervals would be detectable under controlled, experimental conditions. The use of Ovuplant to hasten ovulation in 13 mares, compared to 12 controls, increased (P < 0.05) the interovulatory interval by 6.2 d and suppressed (P < 0.05) plasma concentrations of both luteinizing hormone (LH) and follicle stimulating hormone (FSH) for approximately 11 d. Two mares receiving Ovuplant did not return to estrus within 30 d. In the second experiment, 10 control mares and 10 mares induced to ovulate with Ovuplant were administered GnRH (50 μg) on d 1, 4, 7, and 10 after ovulation. Again, treated mares had a longer (4.4 d, P < 0.05) interovulatory interval and suppressed LH and FSH concentrations in daily plasma samples. The gonadotropin response to GnRH was lower (P < 0.05) in the deslorelin mares on d 1, 4, and 7, indicating a lack of pituitary responsiveness. In the third experiment, 9 stallions and 12 steroid-treated geldings were used to determine if males were potential models for studying the deslorelin-induced gonadotropin suppression. In both cases, treatment with Ovuplant caused an initial rise in both gonadotropins followed by suppression for about 14 d. In the last experiment, 21 mares were used to determine if multiple doses of deslorelin would cause complete ovarian shutdown. Mares received either sham injections, three Ovuplant implants on the first day, or one implant per day for 3 d (n = 7 per group). Treatment with multiple deslorelin implants increased (P < 0.05) the interovulatory interval by 14.8 d and suppressed LH and FSH concentrations for approximately 25 d, however no mares exhibited complete ovarian shutdown. In conclusion, deslorelin acetate implants in horses in the form of Ovuplant induce short-term increases in LH and FSH secretion followed by long-term suppression of these concentrations and an insensitivity of the pituitary to GnRH. In a small percentage of mares, long-term ovarian shutdown is a possibility.

Subjects/Keywords: horses; gnrh

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APA (6^th Edition):

Johnson, C. A. (2002). Endocrine and reproductive responses to implants of deslorein acetate in horses. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218

Chicago Manual of Style (16^th Edition):

Johnson, Carrie Ann. “Endocrine and reproductive responses to implants of deslorein acetate in horses.” 2002. Doctoral Dissertation, Louisiana State University. Accessed March 22, 2019. etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218.

MLA Handbook (7^th Edition):

Johnson, Carrie Ann. “Endocrine and reproductive responses to implants of deslorein acetate in horses.” 2002. Web. 22 Mar 2019.

Vancouver:

Johnson CA. Endocrine and reproductive responses to implants of deslorein acetate in horses. [Internet] [Doctoral dissertation]. Louisiana State University; 2002. [cited 2019 Mar 22]. Available from: etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218.

Council of Science Editors:

Johnson CA. Endocrine and reproductive responses to implants of deslorein acetate in horses. [Doctoral Dissertation]. Louisiana State University; 2002. Available from: etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218

6. Andrade, Luís Pedro Mota Pinto de. Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows.

Degree: 2013, Instituto Politécnico de Castelo Branco

URL: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817

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Reproductive efficiency in beef cows is limited by the length of the postpartum anoestrous period wich in turn is controlled by pituitary gonadotrophins and gonadal… (more)

Subjects/Keywords: Beef cattle; Reproduction; GnRH; LH

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APA (6^th Edition):

Andrade, L. P. M. P. d. (2013). Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows. (Thesis). Instituto Politécnico de Castelo Branco. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andrade, Luís Pedro Mota Pinto de. “Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows.” 2013. Thesis, Instituto Politécnico de Castelo Branco. Accessed March 22, 2019. https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andrade, Luís Pedro Mota Pinto de. “Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows.” 2013. Web. 22 Mar 2019.

Vancouver:

Andrade LPMPd. Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows. [Internet] [Thesis]. Instituto Politécnico de Castelo Branco; 2013. [cited 2019 Mar 22]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andrade LPMPd. Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows. [Thesis]. Instituto Politécnico de Castelo Branco; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

7. O'Neil, Meaghan Marie. The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares.

Degree: MS, Physiology of Reproduction, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/169637

► Continuous infusion with native gonadotropin-releasing hormone (GnRH) restores follicular growth and ovulation in approximately 85% of winter anovulatory mares. However, the potential role of estradiol-17β… (more)

▼ Continuous infusion with native gonadotropin-releasing hormone (GnRH) restores follicular growth and ovulation in approximately 85% of winter anovulatory mares. However, the potential role of estradiol-17β (E2) in enhancing pituitary responsiveness to GnRH during this transitional process is unclear. Moreover, the contribution of follicle-stimulating hormone (FSH) to the overall response has not been studied. Objectives herein were to test the hypotheses that (1) continuous treatment with either a low or high dose of E2 enhances pituitary responsiveness to continuous infusion of native GnRH, and (2) the pattern of FSH secretion mimics that of LH. Twenty-four winter anovulatory mares were stratified by body weight and body condition score, and assigned randomly to 1 of 4 treatments (n = 6/treatment) in early February: 1) Control; Silastic® subcutaneous (S.C.) sham pump, 2) GnRH; native GnRH in saline via 14-day Alzet® osmotic pump (100 μg/h, S.C.), 3) GnRH + High E2; GnRH as in 2 plus Compudose® E2 implant, (25.7-mg, S.C.), and 4) GnRH + Low E2; GnRH as in 2 and 3 plus 1/4-length E2 implant (6.425-mg, S.C.). Plasma concentrations of LH, FSH, E2, estrone sulfate, and progesterone were measured in daily blood samples. Mares developing 35-mm follicles were induced to ovulate with human chorionic gonadotropin (hCG). Mares not ovulating within 14 days received a second 14-day GnRH pump. Although acute increases in uterine edema observed within 48 hours indicated implant functionality, plasma E2 and estrone sulfate were increased primarily in associationwith a large, Continuous infusion with native gonadotropin-releasing hormone (GnRH) restores follicular growth and ovulation in approximately 85% of winter anovulatory mares. However, the potential role of estradiol-17β (E2) in enhancing pituitary responsiveness to GnRH during this transitional process is unclear. Moreover, the contribution of follicle-stimulating hormone (FSH) to the overall response has not been studied. Objectives herein were to test the hypotheses that (1) continuous treatment with either a low or high dose of E2 enhances pituitary responsiveness to continuous infusion of native GnRH, and (2) the pattern of FSH secretion mimics that of LH. Twenty-four winter anovulatory mares were stratified by body weight and body condition score, and assigned randomly to 1 of 4 treatments (n = 6/treatment) in early February: 1) Control; Silastic® subcutaneous (S.C.) sham pump, 2) GnRH; native GnRH in saline via 14-day Alzet® osmotic pump (100 μg/h, S.C.), 3) GnRH + High E2; GnRH as in 2 plus Compudose® E2 implant, (25.7-mg, S.C.), and 4) GnRH + Low E2; GnRH as in 2 and 3 plus 1/4-length E2 implant (6.425-mg, S.C.). Plasma concentrations of LH, FSH, E2, estrone sulfate, and progesterone were measured in daily blood samples. Mares developing 35-mm follicles were induced to ovulate with human chorionic gonadotropin (hCG). Mares not ovulating within 14 days received a second 14-day GnRH pump. Although acute increases in uterine edema observed within 48 hours indicated… Advisors/Committee Members: Williams, Gary L (advisor), Cardoso, Rodolfo C (committee member), Welsh, Thomas H (committee member), Hinrichs, Katrin (committee member).

Subjects/Keywords: Mare; winter anovulatory; GnRH; E2

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APA (6^th Edition):

O'Neil, M. M. (2017). The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/169637

Chicago Manual of Style (16^th Edition):

O'Neil, Meaghan Marie. “The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares.” 2017. Masters Thesis, Texas A&M University. Accessed March 22, 2019. http://hdl.handle.net/1969.1/169637.

MLA Handbook (7^th Edition):

O'Neil, Meaghan Marie. “The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares.” 2017. Web. 22 Mar 2019.

Vancouver:

O'Neil MM. The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/1969.1/169637.

Council of Science Editors:

O'Neil MM. The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/169637

8. Márlon de Vasconcelos Azevedo. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.

Degree: 2012, Universidade Federal Rural de Pernambuco

URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

► Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência… (more)

▼ Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência reprodutiva e resultados econômicos em um programa comercial de transferência de embriões, no agreste pernambucano. No experimento 01 foram utilizadas quinze éguas doadoras de embrião sendo dez da Raça Quarto de Milha e cinco da raça Mangalarga Machador, com idade entre oito e vinte anos. O grupo 1 (G1) iniciou-se com o monitoramento do ciclo estral, inseminação atrificial durante o estro e colheita de embriões no oitavo dia pós ovulação. O Grupo 2 (G2) constituido pelas mesmas éguas do G1, tendo início com a aplicação de 7,5 mg de dinoprost-trometamina, o monitoramento dos ovários através do ultarassom, duas vezes ao dia até a detecção do maior folículo com diâmetro de 23 a 25 mm e o segundo >18 mm, momento inicial do tratamento superovulatório, com 100 μg de acetato de deslorelina, com intervalo de 12 h, até quando o maior folículo atingisse o diâmetro de 33 a 35 mm, quando foi realizado a indução da ovulação atravez da aplicação de 1000 μg de acetato de deslorelina IM associado a 1000 UI de hCG IV. No experimento 02 foram utilizadas sete éguas doadoras, sendo quatro da Raça Quarto de Milha e três da raça Mangalarga Machador, com idade entre oito a vinte anos. Os grupos G1 e G2 são iguais ao experimento 01, sendo acrescido do G3 que se iniciou após a reaplicação de 7,5 mg de dinoprosttrometamina após a colheita do G2, 48 h após é iniciado o tratamento com 100 μg (1ml) de acetato de deslorelina, com intervalo de 12h, até que o maior folículo atingisse 341 mm de diâmetro ou 6 dias de aplicação, quando foi feita a indução de ovulação com o mesmo protocolo do G2 . No experimento 01 o percentual de éguas com múltiplas ovulações no G1 e G2 foi de (2/15) 13,33% e de (13/15) 86,66%, respectivamente (P<0,005). Também foi observado um incremento no número de embriões recuperados, sendo no G1 apenas nove, enquanto no G2 foram de dezesseis embriões (P<0,005), com uma média de recuperação de 0,60,48 no G1 e de 1,0660,497 no G2 (P>0,005); e o percentual de recuperação embrionária foi de 60% G1 e de 80% no G2 (P<0,005). No experimento 02 o percentual de éguas com múltiplas ovulações no G1, G2 e G3 foi de 14,28% (1/7), 100,00% (7/7) e 0% (0/7) respectivamente (P<0,005). Foi observado um incremento no número de embriões recuperados sendo no G1 três, G2 seis e G3 zero (P<0,005), com uma média de recuperação embrionária de 0,43 0,53 no G1, de 0,86 0,38 no G2 e 0 G3 (P>0,005); com um percentual de recuperação embrionária de 43% G1, de 85,71% no G2 e 0% no G3 (P<0,005). Os resultados obtidos nestes experimentos permitem concluir que a administração de GnRH (deslorelina) em baixa dose em éguas doadoras de embriões, interferiu positivamente no crescimento do folículo dominante e do segundo folículo, aumentando a incidência de ovulações múltiplas. Incrementando a taxa de recuperação embrionária por ciclo, melhorando a eficiência reprodutiva das doadoras… Advisors/Committee Members: Paulo Fernandes de Lima, Marcos Antonio Lemos de Oliveira, Adriana Wanderley Taveiros, Claudio Coutinho Bartolomeu.

Subjects/Keywords: Reprodução; Biotecnologias; Equinos; GnRH; MEDICINA VETERINARIA; Biotechnology; Reproduction; Equine; GnRH

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Azevedo, M. d. V. (2012). Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 22, 2019. http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Web. 22 Mar 2019.

Vancouver:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2012. [cited 2019 Mar 22]. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Thesis]. Universidade Federal Rural de Pernambuco; 2012. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Márlon de Vasconcelos Azevedo. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.

Degree: 2012, Universidade Federal Rural de Pernambuco

URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

► Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência… (more)

▼ Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência reprodutiva e resultados econômicos em um programa comercial de transferência de embriões, no agreste pernambucano. No experimento 01 foram utilizadas quinze éguas doadoras de embrião sendo dez da Raça Quarto de Milha e cinco da raça Mangalarga Machador, com idade entre oito e vinte anos. O grupo 1 (G1) iniciou-se com o monitoramento do ciclo estral, inseminação atrificial durante o estro e colheita de embriões no oitavo dia pós ovulação. O Grupo 2 (G2) constituido pelas mesmas éguas do G1, tendo início com a aplicação de 7,5 mg de dinoprost-trometamina, o monitoramento dos ovários através do ultarassom, duas vezes ao dia até a detecção do maior folículo com diâmetro de 23 a 25 mm e o segundo >18 mm, momento inicial do tratamento superovulatório, com 100 μg de acetato de deslorelina, com intervalo de 12 h, até quando o maior folículo atingisse o diâmetro de 33 a 35 mm, quando foi realizado a indução da ovulação atravez da aplicação de 1000 μg de acetato de deslorelina IM associado a 1000 UI de hCG IV. No experimento 02 foram utilizadas sete éguas doadoras, sendo quatro da Raça Quarto de Milha e três da raça Mangalarga Machador, com idade entre oito a vinte anos. Os grupos G1 e G2 são iguais ao experimento 01, sendo acrescido do G3 que se iniciou após a reaplicação de 7,5 mg de dinoprosttrometamina após a colheita do G2, 48 h após é iniciado o tratamento com 100 μg (1ml) de acetato de deslorelina, com intervalo de 12h, até que o maior folículo atingisse 341 mm de diâmetro ou 6 dias de aplicação, quando foi feita a indução de ovulação com o mesmo protocolo do G2 . No experimento 01 o percentual de éguas com múltiplas ovulações no G1 e G2 foi de (2/15) 13,33% e de (13/15) 86,66%, respectivamente (P<0,005). Também foi observado um incremento no número de embriões recuperados, sendo no G1 apenas nove, enquanto no G2 foram de dezesseis embriões (P<0,005), com uma média de recuperação de 0,60,48 no G1 e de 1,0660,497 no G2 (P>0,005); e o percentual de recuperação embrionária foi de 60% G1 e de 80% no G2 (P<0,005). No experimento 02 o percentual de éguas com múltiplas ovulações no G1, G2 e G3 foi de 14,28% (1/7), 100,00% (7/7) e 0% (0/7) respectivamente (P<0,005). Foi observado um incremento no número de embriões recuperados sendo no G1 três, G2 seis e G3 zero (P<0,005), com uma média de recuperação embrionária de 0,43 0,53 no G1, de 0,86 0,38 no G2 e 0 G3 (P>0,005); com um percentual de recuperação embrionária de 43% G1, de 85,71% no G2 e 0% no G3 (P<0,005). Os resultados obtidos nestes experimentos permitem concluir que a administração de GnRH (deslorelina) em baixa dose em éguas doadoras de embriões, interferiu positivamente no crescimento do folículo dominante e do segundo folículo, aumentando a incidência de ovulações múltiplas. Incrementando a taxa de recuperação embrionária por ciclo, melhorando a eficiência reprodutiva das doadoras… Advisors/Committee Members: Paulo Fernandes de Lima, Marcos Antonio Lemos de Oliveira, Adriana Wanderley Taveiros, Claudio Coutinho Bartolomeu.

Subjects/Keywords: Reprodução; Biotecnologias; Equinos; GnRH; MEDICINA VETERINARIA; Biotechnology; Reproduction; Equine; GnRH

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Azevedo, M. d. V. (2012). Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 22, 2019. http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Web. 22 Mar 2019.

Vancouver:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2012. [cited 2019 Mar 22]. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Thesis]. Universidade Federal Rural de Pernambuco; 2012. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

10. Joseph, Nerine Theresa. Characterisation of the tissue-specific expression, pharmacology and signalling cascades activated by chicken GnRH receptor subtypes suggested evolutionary specialisation of type III cGnRH receptor function.

Degree: PhD, 2010, University of Edinburgh

URL: http://hdl.handle.net/1842/4802

► Variant GnRH ligand and receptor subtypes have been identified in a number of non-mammalian vertebrate species, however research into avian species GnRH systems is lacking.… (more)

▼ Variant GnRH ligand and receptor subtypes have been identified in a number of non-mammalian vertebrate species, however research into avian species GnRH systems is lacking. Two isoforms of GnRH are present in the domestic chicken, the evolutionarily conserved GnRH-II and diverged cGnRH-I. The expression of two GnRH ligands parallels the expression of two chicken GnRH receptor subtypes; cGnRH-R-I and the novel cGnRH-R-III. The occurrence of two isoforms of the receptor in the chicken raises questions about their specific biological functions and interactions with the two ligands. Differential roles for these molecules in regulating gonadotrophin secretion or other functions are currently unclear. To investigate this, cGnRH-R-III cDNA was cloned from a broiler chicken anterior pituitary gland and its structure and expression was compared with cGnRH-R-I. Expression profiling of cGnRH-R-III cDNA showed that it is predominantly expressed in the anterior pituitary, approximately 1400 times more abundantly than cGnRH-R-I suggesting that cGnRH-R-III is the predominant regulator of chicken gonadotrophin synthesis and secretion. Additionally, pronounced sex and age differences existed, with higher pituitary cGnRH-R-III mRNA levels in sexually mature females versus juvenile females. In contrast, higher mRNA expression levels occurred in juvenile males compared to sexually mature males. Determination of ligand-binding selectivity and the level of cGnRH-R-III activation in response to the endogenous ligands, cGnRH-I and GnRH-II, was anticipated as facilitating the elucidation of the physiological roles of the receptor subtypes. Additionally, the development of analogs that differentially promote or inhibit activation of the receptor subtypes may be valuable tools for determining the role of receptor types in the regulation of gonadotrophin production. To investigate this, pharmacological profiling of cGnRH-R-III in terms of ligand-binding selectivity and inositol phosphate production in response to GnRH analogs was determined in comparison with the pharmacological profile of cGnRH-R-I. Functional studies in COS-7 cells indicated that cGnRH-R-III has a higher binding affinity for GnRH-II than cGnRH-I (IC50: 0.57 v 19.8 nM) and more potent stimulation of inositol phosphate production (EC50: 0.8 v 4.38 nM). Similar results were found for cGnRH-R-I, (IC50: 0.51 v 10.8 nM) and (EC50: 0.7 v 2.8 nM). Mammalian receptor antagonist 27 distinguished between cGnRH-R-I and cGnRH-R-III (IC50: 2.3 v 351 nM), and application of this synthetic peptide may facilitate delineation of receptor subtype function either in-vitro or in-vivo. The length of the C-terminal tail of cGnRH-R-III is 8 residues longer than that of cGnRH-R-I and this observation stimulated investigation of differences in ligand-induced internalisation between the two receptor subtypes. The initial rate of receptor internalisation was faster for cGnRH-R-III than for cGnRH-R-I (26%.min-1 v 15.8%.min-1). Although proteins encoded by cGnRH-R-III splice variants do not bind GnRH…

Subjects/Keywords: 571.74; chicken; GnRH; GnRH-receptor; GPCR; expression; pharmacology; signalling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Joseph, N. T. (2010). Characterisation of the tissue-specific expression, pharmacology and signalling cascades activated by chicken GnRH receptor subtypes suggested evolutionary specialisation of type III cGnRH receptor function. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/4802

Chicago Manual of Style (16^th Edition):

Joseph, Nerine Theresa. “Characterisation of the tissue-specific expression, pharmacology and signalling cascades activated by chicken GnRH receptor subtypes suggested evolutionary specialisation of type III cGnRH receptor function.” 2010. Doctoral Dissertation, University of Edinburgh. Accessed March 22, 2019. http://hdl.handle.net/1842/4802.

MLA Handbook (7^th Edition):

Joseph, Nerine Theresa. “Characterisation of the tissue-specific expression, pharmacology and signalling cascades activated by chicken GnRH receptor subtypes suggested evolutionary specialisation of type III cGnRH receptor function.” 2010. Web. 22 Mar 2019.

Vancouver:

Joseph NT. Characterisation of the tissue-specific expression, pharmacology and signalling cascades activated by chicken GnRH receptor subtypes suggested evolutionary specialisation of type III cGnRH receptor function. [Internet] [Doctoral dissertation]. University of Edinburgh; 2010. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/1842/4802.

Council of Science Editors:

Joseph NT. Characterisation of the tissue-specific expression, pharmacology and signalling cascades activated by chicken GnRH receptor subtypes suggested evolutionary specialisation of type III cGnRH receptor function. [Doctoral Dissertation]. University of Edinburgh; 2010. Available from: http://hdl.handle.net/1842/4802

11. Junqueira, Flávia Raquel Rosa. Uso do análogo do GnRH para diagnóstico de puberdade precoce.

Degree: Mestrado, Ginecologia e Obstetrícia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;

►

Introdução - A puberdade precoce verdadeira ou dependente de GnRH apresenta importante morbidade: a baixa estatura, conseqüência da rápida progressão da idade óssea, além das… (more)

▼

Introdução - A puberdade precoce verdadeira ou dependente de GnRH apresenta importante morbidade: a baixa estatura, conseqüência da rápida progressão da idade óssea, além das seqüelas psico-emocionais do desenvolvimento sexual secundário precoce. Daí a importância da realização de um diagnóstico precoce e preciso, a fim de que a terapêutica adequada seja instituída o quanto antes. O uso do análogo do GnRH (aGnRH) em teste diagnóstico vem sendo utilizado com este objetivo. Neste estudo avaliou-se os valores de corte para o diagnóstico de puberdade precoce verdadeira, usando-se o teste do aGnRH. Material e métodos - Estudo prospectivo, com 44 meninas, com desenvolvimento dos caracteres sexuais secundários antes dos 8 anos de idade, atendidas no Ambulatório de Ginecologia Infanto-Puberal do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Realizou-se, em todos os casos, o teste do aGnRH, que consistiu na coleta de amostra sanguínea basal para dosagem de FSH e LH, seguida da aplicação subcutânea de 500µg de acetato de leuprolida (Lupron®). Novas amostras sanguíneas foram realizadas após 3 horas, para dosagem de FSH e LH, e após 24 horas da aplicação, para dosagem de estradiol Compararam-se os níveis de LH e FSH basais, de 3 horas e a relação LH/FSH obtida, além do estradiol de 24h, com a evolução clínica das pacientes. Este foi o padrão ouro utilizado para análise do teste, sendo que, após 6 meses, as pacientes foram divididas em 2 grupos: puberdade progressiva (puberdade precoce verdadeira) e não-progressiva. Para análise estatística, utilizou-se curvas ROC, estabelecendo-se sensibilidade, especificidade e melhor nível de corte para o diagnóstico de puberdade precoce verdadeira, para os diferentes critérios analisados. Além disso, avaliou-se a concordância entre os diversos tipos de análise do teste, através do coeficiente kappa. Resultados - O LH de 3 horas apresentou valor de corte > 4,5 mUI/mL, sensibilidade 59,1% e especificidade 86,4%, com área sobre a curva de 0,723. O valor de kappa foi de 0,45, com concordância de 0,73. O estradiol de 24 horas apresentou valor de corte > 40,6 pg/mL, sensibilidade 70% e especificidade 73,7%, com área sobre a curva de 0,703. O valor de kappa foi de 0,436, com concordância de 0,718. Dentre todos os critérios analisados, o melhor deles foi a relação LH/FSH de 3 horas, com valor de corte > 0,14, sensibilidade 72,7% e especificidade 77,3%, com área sobre a curva de 0,771. O valor de kappa foi de 0,5, com concordância de 0,75. Conclusões - Em nossa avaliação, a relação LH/FSH de 3 horas foi superior ao valor de LH de 3 horas ou estradiol de 24 horas, que haviam sidos os melhores critérios diagnósticos no trabalho pioneiro na utilização deste teste.

Introduction - True or GnRH-dependent precocious puberty involves important morbidity such as short stature due to the rapid progression of bone age, as well as psycho-emotional sequels of precocious secondary sexual development. Thus, it is important to make an early and precise diagnosis…

Advisors/Committee Members: Reis, Rosana Maria dos.

Subjects/Keywords: análogo do GnRH; diagnosis; diagnóstico; GnRH analogue; Precocious puberty; Puberdade precoce

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Junqueira, F. R. R. (2007). Uso do análogo do GnRH para diagnóstico de puberdade precoce. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;

Chicago Manual of Style (16^th Edition):

Junqueira, Flávia Raquel Rosa. “Uso do análogo do GnRH para diagnóstico de puberdade precoce.” 2007. Masters Thesis, University of São Paulo. Accessed March 22, 2019. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;.

MLA Handbook (7^th Edition):

Junqueira, Flávia Raquel Rosa. “Uso do análogo do GnRH para diagnóstico de puberdade precoce.” 2007. Web. 22 Mar 2019.

Vancouver:

Junqueira FRR. Uso do análogo do GnRH para diagnóstico de puberdade precoce. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2019 Mar 22]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;.

Council of Science Editors:

Junqueira FRR. Uso do análogo do GnRH para diagnóstico de puberdade precoce. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;

Universidade do Rio Grande do Sul

12. Montenegro, Ivan Sereno. Avaliação da estimulação ovariana com uso de análogos do gnrh.

Degree: 2012, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/56618

►

Introdução: Novos medicamentos trouxeram um considerável aumento na chance de gravidez no momento em que a estimulação ovariana controlada permitiu aumentar o número de oócitos… (more)

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Introdução: Novos medicamentos trouxeram um considerável aumento na chance de gravidez no momento em que a estimulação ovariana controlada permitiu aumentar o número de oócitos a serem recuperados e, consequentemente, um maior número de embriões para serem transferidos. Os análogos de hormônio liberador de gonadotrofina, em associação com gonadotrofinas, trouxeram a solução para um dos problemas da estimulação ovariana controlada: o pico precoce de hormônio luteinizante, que prejudicava a coleta de oócitos em torno de 20% dos casos. Rotineiramente, usamos o protocolo com agonista, mas a possibilidade do uso de antagonistas resulta em maior facilidade de manuseio pelo usuário, envolve um único menstrual ciclo e dispensa orientação para uso de drogas, no final do ciclo anterior. Assim, buscamos avaliar o uso dos dois protocolos e analisar seus resultados. Objetivos: Análise e comparação de dados entre dois protocolos de indução (longo com agonista e flexível com antagonista) em pacientes submetidas a técnicas de reprodução assistida na SEGIR – Serviço de Ecografica, Genética e Reprodução Assistida – Porto Alegre. Métodos: Estudo transversal comparando os resultados intermediários com o uso de dois diferentes protocolos de estimulação ovariana com de agonista versus antagonista do hormônio liberador de gonadotrofina para técnicas de reprodução assistida. A análise estatística dos dados recuperados (idade, índice de massa corpórea, número de oócitos recuperados, número de oócitos fertilizados, número de oócitos clivados, dose total de FSH utilizada e ocorrência de síndrome do hiperestímulo ovariano) foi realizada através de teste t de Student para dados paramétricos e análise de covariância para as variáveis dependentes, calculados com o programa SPSS 16.0. Resultados: Um total de 50 pacientes preencheram os critérios para inclusão no estudo entre janeiro e março no ano de 2010, sendo 25 em cada grupo. Houve diferença estatística apenas na idade média entre os grupos (p=0,031). Não houve diferença estatística para os demais dados analizados (índice de massa corpórea, número de oócitos recuperados, número de oócitos fertilizados, número de oócitos clivados e dose de FSH utilizada) entre os grupos. Não houve casos de síndrome do hiperestímulo ovariano. Conclusão: Ambos protocolos os são iguais em termos de resultados. O agonista tem vantagens sobre o agendamento do procedimento, mas leva muito tempo para começar a estimulação e tem a possibilidade de iniciar a medicação em uma paciente grávida. Somado a isso, temos a possibilidade de ter síndrome do hiperestímulo ovariano como complicação. No grupo antagonista, está claro a maior facilidade de uso da medicação e o início mais rápido da estimulação ovariana.

Background: New medications have brought a considerable increase in the chance of pregnancy at the time that controlled ovarian stimulation allowed an increase in the number of oocytes to be recruited and, consequently, a greater number of embryos to be transferred. The gonadotropin-releasing hormone analogues, in…

Advisors/Committee Members: Passos, Eduardo Pandolfi.

Subjects/Keywords: IVF; Técnicas de reprodução assistida; Infertilidade; Outcome/controlled; Ovarian stimulation/GnRH; Indução da ovulação; Analogue/GnRH; Agonist/GnRH; Antagonist

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Montenegro, I. S. (2012). Avaliação da estimulação ovariana com uso de análogos do gnrh. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/56618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Montenegro, Ivan Sereno. “Avaliação da estimulação ovariana com uso de análogos do gnrh.” 2012. Thesis, Universidade do Rio Grande do Sul. Accessed March 22, 2019. http://hdl.handle.net/10183/56618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Montenegro, Ivan Sereno. “Avaliação da estimulação ovariana com uso de análogos do gnrh.” 2012. Web. 22 Mar 2019.

Vancouver:

Montenegro IS. Avaliação da estimulação ovariana com uso de análogos do gnrh. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2012. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10183/56618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Montenegro IS. Avaliação da estimulação ovariana com uso de análogos do gnrh. [Thesis]. Universidade do Rio Grande do Sul; 2012. Available from: http://hdl.handle.net/10183/56618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Benz, Nathalie. Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title.

Degree: Docteur es, Biologie-santé, 2013, Brest

URL: http://www.theses.fr/2013BRES0021

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Introduction : La mucoviscidose est une maladie génétique autosomale récessive causée par des mutations dans le gène CFTR (cystic fibrosis transmembrane conductance regulator). Ce dernier… (more)

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Introduction : La mucoviscidose est une maladie génétique autosomale récessive causée par des mutations dans le gène CFTR (cystic fibrosis transmembrane conductance regulator). Ce dernier code un canal chlorure AMPc-dépendant localisé dans la membrane apicale des cellules épithéliales, dont l’activité est régulée par de nombreuses interactions protéine-protéine. Dans le cadre de la recherche de nouveaux partenaires du CFTR, une interaction directe entre le canal (sauvage et muté F508del) etl’annexine A5 (AnxA5) a été mise en évidence dans notre laboratoire. Des stratégies de sur et de sousexpression nous ont également permis d’établir un lien fonctionnel entre les deux protéines. En effet, nos travaux montrent que les sécrétions ioniques dépendantes du CFTR sont corrélées au niveau d’expression intracellulaire de l’AnxA5. Par ailleurs, une élévation des courants médiés par le CFTR ainsi qu’une augmentation de la quantité de canaux dans la membrane plasmique sont observées suite à la surexpression de l’AnxA5 dans des cellules exprimant le CFTR muté F508del.But de l’étude : Au vu de ces observations, l’AnxA5 apparaît comme une cible potentielle pour la correction de certains défauts engendrés par la mutation F508del. Une piste thérapeutique pourrait être l’identification de composés capables d’augmenter son expression dans des cellules épithéliales exprimant le mutant F508del de la protéine CFTR. Considérant les informations fournies par la littérature, notre choix s’est porté sur la GnRH (gonadotropin-releasing hormone), molécule utilisée en thérapeutique humaine depuis plus de 25 ans. Ainsi, nous avons évalué l’effet de la GnRH sur la modulation de l’expression de l’AnxA5 et sur le transport ionique dépendant du CFTR dans nos différents modèles d’étude Résultats : Outre la présence du récepteur de la GnRH dans nos modèles cellulaires, nous montrons également que l’expression de l’AnxA5 y est augmentée dès 60 minutes de traitement avec l’hormone (1 nM). De plus, comparativement à des cellules non stimulées, des cellules prétraitées avec la GnRH présentent une hausse significative des sorties actives d’iodure, corrélant avec une augmentation de la quantité de CFTR à la surface cellulaire. Ces observations ont été faites dans les modèles exprimant le CFTR muté F508del ainsi que dans ceux exprimant le CFTR sauvage. Conclusion : Dans nos modèles et selon nos conditions de stimulation, un traitement avec la GnRH augmente l’expression intracellulaire de l’AnxA5 et conduit à une élévation des sécrétions ioniques médiées par le canal CFTR. Néanmoins, au vu de la multitude de voies de signalisation susceptibles d’être activées et de gènes pouvant être régulés suite à la liaison de la GnRH sur son récepteur, l’effet observé sur l’AnxA5 ne représente probablement pas le seul évènement cellulaire à l’origine de l’impact positif enregistré sur l’activité du canal CFTR.

Background: Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene,…

Advisors/Committee Members: Férec, Claude (thesis director).

Subjects/Keywords: CFTR; Mutation F508del; Transport ionique; AnxA5; GnRH; Récepteur de la GnR; CFTR; F508del mutation; Ion transport; AnxA5; GnRH; GnRH recepto

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benz, N. (2013). Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title. (Doctoral Dissertation). Brest. Retrieved from http://www.theses.fr/2013BRES0021

Chicago Manual of Style (16^th Edition):

Benz, Nathalie. “Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title.” 2013. Doctoral Dissertation, Brest. Accessed March 22, 2019. http://www.theses.fr/2013BRES0021.

MLA Handbook (7^th Edition):

Benz, Nathalie. “Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title.” 2013. Web. 22 Mar 2019.

Vancouver:

Benz N. Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title. [Internet] [Doctoral dissertation]. Brest; 2013. [cited 2019 Mar 22]. Available from: http://www.theses.fr/2013BRES0021.

Council of Science Editors:

Benz N. Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title. [Doctoral Dissertation]. Brest; 2013. Available from: http://www.theses.fr/2013BRES0021

Texas A&M University

14. Korthanke, Curtis M. Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare.

Degree: MS, Physiology of Reproduction, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/156795

► Seasonal reproduction is highly correlated with circulating concentrations of luteinizing hormone (LH) in mares and low peripheral concentrations of LH serve as the primary basis… (more)

▼ Seasonal reproduction is highly correlated with circulating concentrations of luteinizing hormone (LH) in mares and low peripheral concentrations of LH serve as the primary basis for ovarian quiescence during the non-breeding season. Four experiments were conducted to investigate the effects and underlying relationships of 1-adamantanecarbonyl-RF-NH2 (RF9) and estradiol in the regulation of LH secretion. During the luteal phase of the estrous cycle (Experiment 1), mares were treated with two bolus intravenous injections of RF9 (0.2 and 0.4 mg/kg, respectively) within a 1-h interval to determine effects on secretion of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Dose-dependent increases (P < 0.05) in both FSH and LH concentrations in response to RF9 were observed. Due to an inability to validate the GnRH radioimmunoassay, changes in secretion of GnRH could not be determined. In Experiment 2, the effect of estradiol-17? (estradiol) pretreatment on responsiveness to RF9 was examined in winter anovulatory mares. Mares received a single intramuscular injection of corn oil of 5 mg estradiol in corn oil followed by a bolus injection of RF9 18 h later. Estradiol pretreatment increased (P < 0.05) peak concentrations of LH in response to RF9. The half-life of RF9 in the circulation of the mare following peripheral administration was determined in Experiment 3 using liquid chromatography and mass spectrometry. Intravenously-injected RF9 was rapidly removed from the mare?s circulatory system; exhibiting a half-life of approximately 40 min. Finally, the role of estradiol and its interaction with GnRH in regulating secretion of LH in anovulatory mares during increasing photoperiod was examined. In February, mares were assigned to 1 of 4 groups and served as controls (corn oil injections) or received 1 of 3 treatments: daily i.m. injections of estradiol in corn oil for 14 d, continuous subcutaneous treatment with native GnRH for 14 d at a rate of 100 ?g/h, or the estradiol and GnRH treatments in combination. Estradiol alone had no effect on secretion of LH but markedly enhanced (P < 0.0002) responsiveness to GnRH. Due to its short half-life, RF9 may not be useful therapeutically; however, estradiol enhances responsiveness to GnRH and may be useful in treatments designed to accelerate vernal transition. Advisors/Committee Members: Williams, Gary L (advisor), Brinsko, Steven P (committee member), Welsh, Jr., Thomas H (committee member).

Subjects/Keywords: Mare; Anovulatory; Seasonality; Estradiol; RF9; GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Korthanke, C. M. (2016). Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156795

Chicago Manual of Style (16^th Edition):

Korthanke, Curtis M. “Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare.” 2016. Masters Thesis, Texas A&M University. Accessed March 22, 2019. http://hdl.handle.net/1969.1/156795.

MLA Handbook (7^th Edition):

Korthanke, Curtis M. “Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare.” 2016. Web. 22 Mar 2019.

Vancouver:

Korthanke CM. Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/1969.1/156795.

Council of Science Editors:

Korthanke CM. Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/156795

University of Colorado

15. Ramelli, Sarah Leanne. Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons.

Degree: MS, Integrative Physiology, 2014, University of Colorado

URL: http://scholar.colorado.edu/iphy_gradetds/29

► Fibroblast growth factor receptor 1 (Fgfr1) gene mutations can cause deficiencies in gonadotropin-releasing hormone (GnRH) production in humans. GnRH deficiencies cause Kallmann syndrome and… (more)

Subjects/Keywords: Fgfr1; GnRH; reproductive impairment; Endocrinology; Physiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramelli, S. L. (2014). Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons. (Masters Thesis). University of Colorado. Retrieved from http://scholar.colorado.edu/iphy_gradetds/29

Chicago Manual of Style (16^th Edition):

Ramelli, Sarah Leanne. “Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons.” 2014. Masters Thesis, University of Colorado. Accessed March 22, 2019. http://scholar.colorado.edu/iphy_gradetds/29.

MLA Handbook (7^th Edition):

Ramelli, Sarah Leanne. “Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons.” 2014. Web. 22 Mar 2019.

Vancouver:

Ramelli SL. Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons. [Internet] [Masters thesis]. University of Colorado; 2014. [cited 2019 Mar 22]. Available from: http://scholar.colorado.edu/iphy_gradetds/29.

Council of Science Editors:

Ramelli SL. Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons. [Masters Thesis]. University of Colorado; 2014. Available from: http://scholar.colorado.edu/iphy_gradetds/29

University of Otago

16. de Croft, Simon. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse .

Degree: University of Otago

URL: http://hdl.handle.net/10523/5611

► The gonadotropin-releasing hormone (GnRH) neurons are the principal neurons in a network within the hypothalamus that controls fertility in all mammals. GnRH neuron output is… (more)

▼ The gonadotropin-releasing hormone (GnRH) neurons are the principal neurons in a network within the hypothalamus that controls fertility in all mammals. GnRH neuron output is influenced by a wide range of neurotransmitters. One recently-discovered and essential neurotransmitter in this network is known as kisspeptin. Kisspeptin neurons exist in two distinct populations; in the rostral periventricular nucleus of the third ventricle (RP3V) and in the arcuate nucleus (ARN). Based upon anatomical evidence, the RP3V kisspeptin neurons are hypothesized to be important for the positive feedback mechanism of estrogen that drives the preovulatory GnRH surge. Also, anatomical evidence has led to the suggestion that the ARN kisspeptin neuronal population is involved in the negative feedback mechanism that homeostatically suppresses GnRH release and also is important to drive pulsatile GnRH secretion. In my PhD, I have performed experiments to gain functional insight into the roles of these two kisspeptin neuronal populations. To identify kisspeptin neurons, I have used a knock-in kisspeptin-GFP mouse line, in which only kisspeptin neurons should appear fluorescent. I used immunohistochemistry to show that kisspeptin neurons can be identified in vitro using GFP fluorescence. I have then used patch-clamp electrophysiology techniques to describe the spontaneous firing rate of kisspeptin neurons and described this across the estrous cycle. I demonstrate that the spontaneous electrical activity is not strongly subject to fluctuating gonadal steroid levels. Unexpectantly, I have described a male-dominant sex difference in spontaneous firing rate of ARN kisspeptin neurons. Additionally, I show that RP3V kisspeptin neurons are not strongly activated at the time of the GnRH surge when measured in an acute brain slice. ARN kisspeptin neurons are thought to form an interconnected network, and utilize coexpressed neuropeptides to generate a pulsatile output that is important for generating the pulsatile pattern of GnRH release from the hypothalamus. I have used cell-attached and whole-cell electrophysiological recording techniques to show that neurokinin B and dynorphin potently modulate ARN kisspeptin neuronal output in an opposing manner, and described the receptors that mediate these effects. Following this, I have used whole-cell electrophysiological recording techniques to test for functional connections between ARN kisspeptin neurons. Whereas no evidence was found for monosynaptic glutamatergic synaptic connections, some evidence was found for neuropeptidergic neurotransmission between ARN kisspeptin neurons. These results provide electrophysiological data to inform and update theories of kisspeptin neuronal function which are built largely on anatomical data. Advisors/Committee Members: Herbison, Allan (advisor).

Subjects/Keywords: Kisspeptin; Hypothalamus; GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

de Croft, S. (n.d.). Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/5611

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

de Croft, Simon. “Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse .” Doctoral Dissertation, University of Otago. Accessed March 22, 2019. http://hdl.handle.net/10523/5611.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

de Croft, Simon. “Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse .” Web. 22 Mar 2019.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

de Croft S. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse . [Internet] [Doctoral dissertation]. University of Otago; [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10523/5611.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

de Croft S. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse . [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/5611

Note: this citation may be lacking information needed for this citation format:
No year of publication.

17. Cruz Quiroga, Ramiro de la. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis.

Degree: 2018, TDX

URL: http://hdl.handle.net/10550/50501

► La aromatasa juega un papel fundamental en el establecimiento de la calidad ovocitaria, la cual puede estar alterada en las mujeres infértiles con endometriosis. Por… (more)

▼ La aromatasa juega un papel fundamental en el establecimiento de la calidad ovocitaria, la cual puede estar alterada en las mujeres infértiles con endometriosis. Por otro lado, la administración prolongada de agonistas de GnRH previo a la fecundación in vitro en estas pacientes, ha demostrado que aumentaba la tasa de gestación aproximadamente cuatro veces. En este estudio se ha comparado la expresión del gen CYP19A1 ( que codifica a la aromatasa) en las células de la granulosa mural (CGM) y en las células de cúmulo (CC), los niveles de estradiol, testosterona y androstenediona en el líquido folicular, la calidad ovocitaria y embrionaria, y la tasa de fecundación de pacientes con endometriosis pre-tratadas con agonistas de GnRH durante 3 meses frente a placebo previo la realización de la FIV. Las células de la granulosa han sido aisladas y la expresión del gen CYP19A1 ha sido cuantificada mediante reacción en cadena de la polimerasa en tiempo real. Las determinaciones hormonales en el liquido folicular se han hecho mediante inmunoensayo, concretamente mediante quimioluminiscencia. La calidad ovocitaria y embrionaria se ha determinado según parámetros morfológicos. En cuanto a los resultados, no se han detectado diferencias significativas en la expresión del gen de la aromatasa en las dos poblaciones celulares estudiadas (CGM y CC) entre ambos grupos ( CGM: grupo control 1,23 ± 3,19 y grupo de estudio 1,44 ± 1,85; CC: grupo control 2,61 ± 3,96 y grupo de estudio 2,15 ± 2,99). Tampoco se han encontrado diferencias estadísticamente significativas al analizar los resultados teniendo en cuenta la unilateralidad de la lesión endometriósica (ovario afectado con el folículo ipsilateral), la bilateralidad, por muestras pareadas y según el grado de endometriosis. Las concentraciones de las hormonas esteroides en el líquido folicular han sido más bajas en el grupo de estudio, siendo el nivel de testosterona estadísticamente significativa ( 9,38 ± 7,94 ng/ml vs 3,10 ± 2,44 ng/ml, control vs estudio respectivamente, p=0,00). En el análisis detallado de resultados la tendencia de los esteroides foliculares ha sido la misma en los casos de unilateralidad y bilateralidad (siendo la testosterona significativamente más baja en el grupo de estudio vs placebo). En el análisis por muestras pareadas, no ha habido diferencias entre ambos grupos. En el análisis según grado de endometriosis, la significancia estadística de las testosterona se ha evidenciado sólo en las endometriosis mínimas o leves ( control: 9,49 ±6,66 ng/ml vs estudio : 3,59 ± 2,56 ng/ml, p=0,00). No se han encontrado diferencias estadísticas en los parámetros de calidad ovocitaria ni embrionaria al comparar ambos grupos. La tasa de fecundación ha sido más baja y significativa en el grupo de estudio que había recibido el pre-tratamiento con agonistas (control: 77,23 ± 20,44 vs estudio: 58,92 ± 28,25, p =0,03). Se ha comparado también si existían diferencias en la expresión de las CGM frente a las CC en ambos grupos, no encontrando diferencias… Advisors/Committee Members: Monzó Miralles, Ana, Pellicer Martínez, Antonio, Novella Maestre, Edurne, Departament de Pediatria, Obstetrícia i Ginecologia.

Subjects/Keywords: aromatasa; endometriosis; agonistas GnRH; UNESCO::CIENCIAS MÉDICAS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cruz Quiroga, R. d. l. (2018). Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis. (Thesis). TDX. Retrieved from http://hdl.handle.net/10550/50501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cruz Quiroga, Ramiro de la. “Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis.” 2018. Thesis, TDX. Accessed March 22, 2019. http://hdl.handle.net/10550/50501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cruz Quiroga, Ramiro de la. “Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis.” 2018. Web. 22 Mar 2019.

Vancouver:

Cruz Quiroga Rdl. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis. [Internet] [Thesis]. TDX; 2018. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10550/50501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cruz Quiroga Rdl. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10550/50501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Rodríguez Tárrega, Elisabet. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro.

Degree: 2018, TDX

URL: http://hdl.handle.net/10550/55362

► Introducción La endometriosis se define como la presencia de tejido endometrial fuera de la cavidad uterina, que induce una reacción inflamatoria local. La fisiopatología de… (more)

▼ Introducción La endometriosis se define como la presencia de tejido endometrial fuera de la cavidad uterina, que induce una reacción inflamatoria local. La fisiopatología de esta enfermedad no está completamente establecida, aunque parece que existen factores hormonales, genéticos e inmunológicos implicados. Su prevalencia exacta es desconocida, pero se estima que hasta el 10% de las pacientes en edad reproductiva y el 50% de pacientes con esterilidad la padecen. Esta enfermedad se asocia con dolor y esterilidad. En estas pacientes, las adherencias pueden obliterar las trompas de Falopio e impedir la liberación del ovocito; además, la reserva ovárica podría estar disminuida por la presencia de endometriomas y/o su tratamiento quirúrgico, mientras que la respuesta ovárica a la estimulación con gonadotrofinas puede estar disminuida. Sin embargo, estas no son las únicas causas de esterilidad en las pacientes con endometriosis, habiéndose propuesto otros mecanismos. Estos incluyen un ambiente folicular y peritoneal alterado con un aumento de citoquinas, que afecta a la foliculogénesis y origina una disminución de la calidad ovocitaria y embrionaria.. Así mismo, se ha observado una disfunción en las células de la granulosa y un efecto tóxico del estado inflamatorio presente en estas pacientes, mediado por macrófagos, interleuquina-8 y citoquinas pro-inflamatorias secretadas en los implantes endometriósicos. Por último, la función espermática y las tasas de fecundación e implantación están también afectadas por este ambiente inflamatorio. La mejor opción terapéutica para pacientes estériles con endometriosis continua siendo controvertida, pero lo que está claro es que muchas de ellas necesitarán realizar técnicas de reproducción asistida (TRA). Diversos estudios han mostrado peores resultados reproductivos tras TRA en pacientes con endometriosis que en pacientes con otras causas de esterilidad. Por este motivo, distintas investigaciones se han llevado a cabo para encontrar tratamientos adyuvantes que mejoren estos resultados reproductivos en pacientes con endometriosis. En este sentido, los agonistas de GnRH (GnRHa) podrían aumentar la tasa de gestación en estas mujeres. Estos fármacos causan una inhibición del eje hipofisario-gonadal que disminuye el estímulo hormonal para el crecimiento de las lesiones endometriósicas reduciendo la reacción inflamatoria presente en las pacientes con endometriosis. Un pre-tratamiento con agonistas de GnRH durante 3 meses previo a fecundación in vitro (FIV) en pacientes estériles con endometriosis podría mejorar las tasas de gestación clínica al disminuir el efecto perjudicial de las citoquinas y del estrés oxidativo. Hipótesis La administración de un análogo de GnRH durante los tres meses previos a la realización de una FIV en pacientes con endometriosis/endometriomas puede mejorar la respuesta ovárica a la estimulación y las tasas de implantación y de gestación clínica. Objetivos Objetivo principal Evaluar el efecto de la administración de un análogo de GnRH comparado con un… Advisors/Committee Members: Monzó Miralles, Ana, Polo Sánchez, Patrocinio, Departament de Pediatria, Obstetrícia i Ginecologia.

Subjects/Keywords: endometriosis; fiv; agonistas gnrh; UNESCO::CIENCIAS MÉDICAS

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodríguez Tárrega, E. (2018). Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro. (Thesis). TDX. Retrieved from http://hdl.handle.net/10550/55362

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodríguez Tárrega, Elisabet. “Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro.” 2018. Thesis, TDX. Accessed March 22, 2019. http://hdl.handle.net/10550/55362.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodríguez Tárrega, Elisabet. “Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro.” 2018. Web. 22 Mar 2019.

Vancouver:

Rodríguez Tárrega E. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro. [Internet] [Thesis]. TDX; 2018. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10550/55362.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodríguez Tárrega E. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10550/55362

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Rodríguez Tárrega, Elisabet. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro.

Degree: 2018, TDX

URL: http://hdl.handle.net/10803/571108

► Introducción La endometriosis se define como la presencia de tejido endometrial fuera de la cavidad uterina, que induce una reacción inflamatoria local. La fisiopatología de… (more)

▼ Introducción La endometriosis se define como la presencia de tejido endometrial fuera de la cavidad uterina, que induce una reacción inflamatoria local. La fisiopatología de esta enfermedad no está completamente establecida, aunque parece que existen factores hormonales, genéticos e inmunológicos implicados. Su prevalencia exacta es desconocida, pero se estima que hasta el 10% de las pacientes en edad reproductiva y el 50% de pacientes con esterilidad la padecen. Esta enfermedad se asocia con dolor y esterilidad. En estas pacientes, las adherencias pueden obliterar las trompas de Falopio e impedir la liberación del ovocito; además, la reserva ovárica podría estar disminuida por la presencia de endometriomas y/o su tratamiento quirúrgico, mientras que la respuesta ovárica a la estimulación con gonadotrofinas puede estar disminuida. Sin embargo, estas no son las únicas causas de esterilidad en las pacientes con endometriosis, habiéndose propuesto otros mecanismos. Estos incluyen un ambiente folicular y peritoneal alterado con un aumento de citoquinas, que afecta a la foliculogénesis y origina una disminución de la calidad ovocitaria y embrionaria.. Así mismo, se ha observado una disfunción en las células de la granulosa y un efecto tóxico del estado inflamatorio presente en estas pacientes, mediado por macrófagos, interleuquina-8 y citoquinas pro-inflamatorias secretadas en los implantes endometriósicos. Por último, la función espermática y las tasas de fecundación e implantación están también afectadas por este ambiente inflamatorio. La mejor opción terapéutica para pacientes estériles con endometriosis continua siendo controvertida, pero lo que está claro es que muchas de ellas necesitarán realizar técnicas de reproducción asistida (TRA). Diversos estudios han mostrado peores resultados reproductivos tras TRA en pacientes con endometriosis que en pacientes con otras causas de esterilidad. Por este motivo, distintas investigaciones se han llevado a cabo para encontrar tratamientos adyuvantes que mejoren estos resultados reproductivos en pacientes con endometriosis. En este sentido, los agonistas de GnRH (GnRHa) podrían aumentar la tasa de gestación en estas mujeres. Estos fármacos causan una inhibición del eje hipofisario-gonadal que disminuye el estímulo hormonal para el crecimiento de las lesiones endometriósicas reduciendo la reacción inflamatoria presente en las pacientes con endometriosis. Un pre-tratamiento con agonistas de GnRH durante 3 meses previo a fecundación in vitro (FIV) en pacientes estériles con endometriosis podría mejorar las tasas de gestación clínica al disminuir el efecto perjudicial de las citoquinas y del estrés oxidativo. Hipótesis La administración de un análogo de GnRH durante los tres meses previos a la realización de una FIV en pacientes con endometriosis/endometriomas puede mejorar la respuesta ovárica a la estimulación y las tasas de implantación y de gestación clínica. Objetivos Objetivo principal Evaluar el efecto de la administración de un análogo de GnRH comparado con un… Advisors/Committee Members: Monzó Miralles, Ana, Polo Sánchez, Patrocinio, Departament de Pediatria, Obstetrícia i Ginecologia.

Subjects/Keywords: endometriosis; fiv; agonistas gnrh; UNESCO::CIENCIAS MÉDICAS

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodríguez Tárrega, E. (2018). Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro. (Thesis). TDX. Retrieved from http://hdl.handle.net/10803/571108

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodríguez Tárrega, Elisabet. “Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro.” 2018. Thesis, TDX. Accessed March 22, 2019. http://hdl.handle.net/10803/571108.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodríguez Tárrega, Elisabet. “Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro.” 2018. Web. 22 Mar 2019.

Vancouver:

Rodríguez Tárrega E. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro. [Internet] [Thesis]. TDX; 2018. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10803/571108.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodríguez Tárrega E. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10803/571108

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Cruz Quiroga, Ramiro de la. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis.

Degree: 2018, TDX

URL: http://hdl.handle.net/10803/570484

► La aromatasa juega un papel fundamental en el establecimiento de la calidad ovocitaria, la cual puede estar alterada en las mujeres infértiles con endometriosis. Por… (more)

▼ La aromatasa juega un papel fundamental en el establecimiento de la calidad ovocitaria, la cual puede estar alterada en las mujeres infértiles con endometriosis. Por otro lado, la administración prolongada de agonistas de GnRH previo a la fecundación in vitro en estas pacientes, ha demostrado que aumentaba la tasa de gestación aproximadamente cuatro veces. En este estudio se ha comparado la expresión del gen CYP19A1 ( que codifica a la aromatasa) en las células de la granulosa mural (CGM) y en las células de cúmulo (CC), los niveles de estradiol, testosterona y androstenediona en el líquido folicular, la calidad ovocitaria y embrionaria, y la tasa de fecundación de pacientes con endometriosis pre-tratadas con agonistas de GnRH durante 3 meses frente a placebo previo la realización de la FIV. Las células de la granulosa han sido aisladas y la expresión del gen CYP19A1 ha sido cuantificada mediante reacción en cadena de la polimerasa en tiempo real. Las determinaciones hormonales en el liquido folicular se han hecho mediante inmunoensayo, concretamente mediante quimioluminiscencia. La calidad ovocitaria y embrionaria se ha determinado según parámetros morfológicos. En cuanto a los resultados, no se han detectado diferencias significativas en la expresión del gen de la aromatasa en las dos poblaciones celulares estudiadas (CGM y CC) entre ambos grupos ( CGM: grupo control 1,23 ± 3,19 y grupo de estudio 1,44 ± 1,85; CC: grupo control 2,61 ± 3,96 y grupo de estudio 2,15 ± 2,99). Tampoco se han encontrado diferencias estadísticamente significativas al analizar los resultados teniendo en cuenta la unilateralidad de la lesión endometriósica (ovario afectado con el folículo ipsilateral), la bilateralidad, por muestras pareadas y según el grado de endometriosis. Las concentraciones de las hormonas esteroides en el líquido folicular han sido más bajas en el grupo de estudio, siendo el nivel de testosterona estadísticamente significativa ( 9,38 ± 7,94 ng/ml vs 3,10 ± 2,44 ng/ml, control vs estudio respectivamente, p=0,00). En el análisis detallado de resultados la tendencia de los esteroides foliculares ha sido la misma en los casos de unilateralidad y bilateralidad (siendo la testosterona significativamente más baja en el grupo de estudio vs placebo). En el análisis por muestras pareadas, no ha habido diferencias entre ambos grupos. En el análisis según grado de endometriosis, la significancia estadística de las testosterona se ha evidenciado sólo en las endometriosis mínimas o leves ( control: 9,49 ±6,66 ng/ml vs estudio : 3,59 ± 2,56 ng/ml, p=0,00). No se han encontrado diferencias estadísticas en los parámetros de calidad ovocitaria ni embrionaria al comparar ambos grupos. La tasa de fecundación ha sido más baja y significativa en el grupo de estudio que había recibido el pre-tratamiento con agonistas (control: 77,23 ± 20,44 vs estudio: 58,92 ± 28,25, p =0,03). Se ha comparado también si existían diferencias en la expresión de las CGM frente a las CC en ambos grupos, no encontrando diferencias… Advisors/Committee Members: Monzó Miralles, Ana, Pellicer Martínez, Antonio, Novella Maestre, Edurne, Departament de Pediatria, Obstetrícia i Ginecologia.

Subjects/Keywords: aromatasa; endometriosis; agonistas GnRH; UNESCO::CIENCIAS MÉDICAS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cruz Quiroga, R. d. l. (2018). Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis. (Thesis). TDX. Retrieved from http://hdl.handle.net/10803/570484

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cruz Quiroga, Ramiro de la. “Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis.” 2018. Thesis, TDX. Accessed March 22, 2019. http://hdl.handle.net/10803/570484.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cruz Quiroga, Ramiro de la. “Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis.” 2018. Web. 22 Mar 2019.

Vancouver:

Cruz Quiroga Rdl. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis. [Internet] [Thesis]. TDX; 2018. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10803/570484.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cruz Quiroga Rdl. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10803/570484

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kansas State University

21. Spiker, Carlie Rae. Suppression of cyclicity and estrous behavior in mares through immunization against a recombinant gonadotropin-releasing hormone antigen.

Degree: MS, Department of Animal Sciences and Industry, 2009, Kansas State University

URL: http://hdl.handle.net/2097/1660

► Mares in estrus can exhibit erratic and fractious behavior that may adversely affect their ease of handling. Current treatments for suppression of estrus in the… (more)

▼ Mares in estrus can exhibit erratic and fractious behavior that may adversely affect their ease of handling. Current treatments for suppression of estrus in the mare include an oral progestin, Regu-mate® (Hodgson et al., 2005), uterine insertion of a glass ball (Nie et al., 2003), fetal crushing through rectal palpation (Lefranc, 2004) and surgical sterilization (McCue, 2003). Although effective, all of these procedures are problematic for different reasons. Immunization against an endogenous hormone critical to fertility is an attractive alternative in suppressing estrus; especially if it is less intrusive and reversible. This strategy has been demonstrated by targeting gonadotropin-releasing hormone (GnRH) in gilts (Esbenshade and Britt, 1985), ewes (Clarke et al., 1978), mares (Garza et al., 1986), and heifers (Johnson et al., 1988). The antigen developed for this study was produced using the sequence of the bacterial protein, glutathione-S-transferase (GST) linked to three in-tandem repeats of the mammalian GnRH gene coding sequence (GST-GnRH3). Six reproductively sound mares, between the ages of 3 and 8 years, were used in this study. The anti-GnRH group (n=4) received 1 mg of GST-GnRH3 in 2ml of incomplete Freund’s adjuvant (IFA) as the primary injection. Four weeks later, mares received a single booster injection of 0.5mg of GST-GnRH3 in 1ml of IFA. The control mares (n=2) received similar doses of GST protein only, in identical injection volumes of IFA as the anti-GnRH group. The entire duration of the study ran for 22 weeks from early May through September. Ovaries were monitored three times weekly to track follicular growth and ovulation via transrectal ultrasonography. In addition, all mares were exposed to a stallion twice weekly and observed for estrous behavior. Weekly blood samples were collected to evaluate progesterone levels and serum binding of GnRH. The GST-GnRH3 antigen suppressed follicular activity in all treatment mares within 45 days following the second injection. Estrous behavior was suppressed in all but one mare in the anti-GnRH group. When exposed to the stallion, this mare displayed strong estrous behavior for seven weeks despite her lack of ovarian cyclicity. Follicular activity and estrous behavior remained normal in one of the control mares (avg. cycle length = 20 days). For the final 10 weeks of the study, however, the other control mare developed large follicles but failed to ovulate according to the ultrasound data. This mare did not display estrous behavior during this period, and her progesterone levels remained greater than 2 ng/ml for most of the final 10 weeks of the study. Approximately 2 weeks after the booster injection all anti-GnRH mares had progesterone levels of <1ng/ml. GnRH antibody binding peaked two weeks following booster immunization in all treated mares and remained undetectable in control mares throughout the study. The GST-GnRH3 treatment induced GnRH binding, suppressed follicular activity and reduced progesterone concentrations in all four mares.… Advisors/Committee Members: David M. Grieger.

Subjects/Keywords: equine; vaccine; sterilization; GnRH; Agriculture, General (0473)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Spiker, C. R. (2009). Suppression of cyclicity and estrous behavior in mares through immunization against a recombinant gonadotropin-releasing hormone antigen. (Masters Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/1660

Chicago Manual of Style (16^th Edition):

Spiker, Carlie Rae. “Suppression of cyclicity and estrous behavior in mares through immunization against a recombinant gonadotropin-releasing hormone antigen.” 2009. Masters Thesis, Kansas State University. Accessed March 22, 2019. http://hdl.handle.net/2097/1660.

MLA Handbook (7^th Edition):

Spiker, Carlie Rae. “Suppression of cyclicity and estrous behavior in mares through immunization against a recombinant gonadotropin-releasing hormone antigen.” 2009. Web. 22 Mar 2019.

Vancouver:

Spiker CR. Suppression of cyclicity and estrous behavior in mares through immunization against a recombinant gonadotropin-releasing hormone antigen. [Internet] [Masters thesis]. Kansas State University; 2009. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/2097/1660.

Council of Science Editors:

Spiker CR. Suppression of cyclicity and estrous behavior in mares through immunization against a recombinant gonadotropin-releasing hormone antigen. [Masters Thesis]. Kansas State University; 2009. Available from: http://hdl.handle.net/2097/1660

22. Crossard, Elodie. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.

Degree: Docteur es, Sciences de la vie, 2011, Université François-Rabelais de Tours

URL: http://www.theses.fr/2011TOUR4026

►

Le kisspeptine (kp) est un peptide, dérivé du gène kiss-1, jouant un rôle majeur dans le contrôle central de la fonction de reproduction en régulant… (more)

▼

Le kisspeptine (kp) est un peptide, dérivé du gène kiss-1, jouant un rôle majeur dans le contrôle central de la fonction de reproduction en régulant la sécrétion du GnRH chez l’adulte mais également au cours du développement. Les neurones exprimant kiss-1 sont situés dans la région rostrale périventriculaire du 3ème ventricule (RP3V) et le noyau arqué (ARC). L’expression de kiss-1 est hautement régulée par les stéroides sexuels, positivement dans RP3V et négativement dans ARC. Ces deux populations de neurones à kp semblent avoir des rôles différents. Les neurones à kp du RP3V seraient impliqués dans la genèse du pic préovulatoire et ceux de l’ARC dans la régulation de la sécrétion pulsatile de GnRH.L’objectif de la thèse était de déterminer la période de neurogenèse des neurones à kp ainsi que les variations de l’expression de kiss-1 et de kp dans ces deux régions au cours des différentes phases du développement chez le rat mâle et femelle.Nos résultats ont permis de cibler les périodes clés de l’ontogenèse des neurones à kp en montrant 1) que les neurones à kp de l’ARC naissent sur une période étendue à partir du jour embryonnaire (E)12,5; 2) l’existence d’une sous- expression péri-natale du kp dans l’ARC indépendante du sexe; 3) la mise en place, en période néonatale, de différences sexuelles dans les niveaux d’expression et la distribution neuroanatomique du kp; 4) l’existence de régulations péri-pubertaires de kp, dépendantes du sexe et de la région ; 5) la présence de fibres à kp dans des régions hypothalamiques suggère un rôle de kp au-delà de la fonction de reproduction.

Kisspeptin (kp) is a neuropeptide, derived from the kiss-1 gene, which plays a key role in the central control of reproduction by regulating GnRH secretion in adult but also during development. Cells which express kiss-1 are localized in two distincts hypothalamic regions: the rostral peri-ventricular third ventricule area (RP3V) and the arcuate nucleus (ARC). Kiss-1 expression is highly regulated by sex steroids: positively in the RP3V and negatively in the ARC. RP3V kp neurons have been implicated in the pre-ovulatory GnRH surge whereas ARC kp neurons may predominantly act on GnRH secretion pulsatility. The aim of this PhD work was to determine the neurogenesis period of kp neurons and changes of kiss-1 and kp expression in both regions during different stages of development in rats. Our results highlight key periods of kp neurons ontogenesis and show that: 1) ARC kp neurons are born during an extended embryonic neurogenesis period starting at embryonic day (E) 12,5; 2) a sex independent down-regulation of kp occurs during peri-natal period; 3) sex difference in the expression level and neuroanatomique distribution of kp establishes during neo-natal period; 4) kp was regulated during peri-pubertal period in sex and region dependant manner; 5) kp-ir fibers are detected throughout the septo-hypothalamic continuum suggesting that kp could be implicated in other functions than reproductive function.

Advisors/Committee Members: Duittoz, Anne (thesis director), Franceschini-Laurent, Isabelle (thesis director).

Subjects/Keywords: GnRH; Kisspeptin; Rat; Neurogenesis; Sex differentiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Crossard, E. (2011). Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2011TOUR4026

Chicago Manual of Style (16^th Edition):

Crossard, Elodie. “Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.” 2011. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 22, 2019. http://www.theses.fr/2011TOUR4026.

MLA Handbook (7^th Edition):

Crossard, Elodie. “Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.” 2011. Web. 22 Mar 2019.

Vancouver:

Crossard E. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2011. [cited 2019 Mar 22]. Available from: http://www.theses.fr/2011TOUR4026.

Council of Science Editors:

Crossard E. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2011. Available from: http://www.theses.fr/2011TOUR4026

Duquesne University

23. Wack, Corina. Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders.

Degree: PhD, Biological Sciences, 2011, Duquesne University

URL: https://dsc.duq.edu/etd/1321

► Pheromones are chemosensory cues released by an individual to cause a behavioral or physiological change in a conspecific. These changes can range from increasing a… (more)

▼ Pheromones are chemosensory cues released by an individual to cause a behavioral or physiological change in a conspecific. These changes can range from increasing a female's receptivity to altering hormone secretions in the body. The red-legged salamander (Plethodon shermani) is an emerging non-mammalian model for understanding the evolution of chemical communication due to their well-characterized pheromones. Plethodontid salamanders secrete pheromones from their submandibular gland, called mental gland pheromones. Previous studies showed that mental gland pheromones increased receptivity in females during courtship and also increased corticosterone (CORT) concentrations in males. CORT is a metabolic hormone that mediates vertebrate stress responses. To further understand the neuroendocrine mechanisms involved in behavioral and physiological responses to pheromones, I conducted several experiments. First, I investigated whether pheromones altered levels of two neuromodulators in the brain, gonadotropin-releasing hormone (GnRH) and arginine vasotocin (AVT) in P. shermani. GnRH is both a neuromodulator and hormone, and has strong effects on reproductive behavior. Additionally, AVT has broad behavioral effects in vertebrates, particularly in newts. I found that application of mental gland pheromones altered the number of GnRH-immunoreactive neurons, but had no effect on the number of AVT-immunoreactive neurons. Second, I examined the effects of AVT and AVP antagonist on courtship and mating in dusky salamanders (Desmognathus spp.). There was no effect of AVT or antagonist on reproductive behaviors in dusky salamanders. Finally, I investigated the role of increased CORT concentrations in male P. shermani. I first validated a non-invasive method to transdermally deliver CORT through a dermal patch. I then used dermal patches to exogenously elevate plasma CORT and examine the effects of CORT on behavior (activity and chemoinvestigation) and metabolism. CORT had no effect on behavior, but increased metabolic rates in male red-legged salamanders. Together these studies provide insight into the mechanisms by which pheromones work to alter behaviors and physiological functions in vertebrates. Advisors/Committee Members: Sarah K. Woodley, Philip E. Auron, Richard P. Elinson, Timothy Smith.

Subjects/Keywords: Corticosterone; GnRH; Metabolism; Salamander; Stress; Vasotocin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wack, C. (2011). Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders. (Doctoral Dissertation). Duquesne University. Retrieved from https://dsc.duq.edu/etd/1321

Chicago Manual of Style (16^th Edition):

Wack, Corina. “Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders.” 2011. Doctoral Dissertation, Duquesne University. Accessed March 22, 2019. https://dsc.duq.edu/etd/1321.

MLA Handbook (7^th Edition):

Wack, Corina. “Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders.” 2011. Web. 22 Mar 2019.

Vancouver:

Wack C. Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders. [Internet] [Doctoral dissertation]. Duquesne University; 2011. [cited 2019 Mar 22]. Available from: https://dsc.duq.edu/etd/1321.

Council of Science Editors:

Wack C. Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders. [Doctoral Dissertation]. Duquesne University; 2011. Available from: https://dsc.duq.edu/etd/1321

Angelo State University

24. Boenig, Michael Wade. The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers.

Degree: MS, Animal Science, 2012, Angelo State University

URL: http://hdl.handle.net/2346.1/30026

► The objective of this study was to determine if a pre synchronization gonadotropin releasing hormone (GnRH) injection would increase conception percentages in postpartum anestrous angus… (more)

Subjects/Keywords: gonadotropin; Angus; GnRH; conception; anestrous angus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boenig, M. W. (2012). The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers. (Masters Thesis). Angelo State University. Retrieved from http://hdl.handle.net/2346.1/30026

Chicago Manual of Style (16^th Edition):

Boenig, Michael Wade. “The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers.” 2012. Masters Thesis, Angelo State University. Accessed March 22, 2019. http://hdl.handle.net/2346.1/30026.

MLA Handbook (7^th Edition):

Boenig, Michael Wade. “The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers.” 2012. Web. 22 Mar 2019.

Vancouver:

Boenig MW. The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers. [Internet] [Masters thesis]. Angelo State University; 2012. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/2346.1/30026.

Council of Science Editors:

Boenig MW. The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers. [Masters Thesis]. Angelo State University; 2012. Available from: http://hdl.handle.net/2346.1/30026

25. Silva, Guilherme Costa de Oliveira e. Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus).

Degree: PhD, Reprodução Animal, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;

►

A falta de conhecimento sobre a reprodução do avestruz dificulta o desenvolvimento da sua criação comercial devido a uma baixa eficiência reprodutiva. O objetivo deste… (more)

▼

A falta de conhecimento sobre a reprodução do avestruz dificulta o desenvolvimento da sua criação comercial devido a uma baixa eficiência reprodutiva. O objetivo deste estudo foi avaliar a ação da aplicação de um análogo de GnRH (lecirelina - Gestran Plus®) na indução da ovulação em fêmeas adultas de avestruz. Primeiramente, um grupo de 10 fêmeas foi avaliado para determinação de seu status reprodutivo, por meio da mensuração das concentrações séricas de estradiol e progesterona (coletas de sangue 3x/semana); da observação do comportamento reprodutivo; da determinação do desenvolvimento ovariano por exame ultrassonográfico (a cada 21 dias); e pela postura de ovos. Após o término desta primeira fase, as fêmeas foram separadas em grupo controle (n = 3) e grupo tratado (n = 6). A cada semana, 2 fêmeas do grupo tratado eram selecionadas para a aplicação intramuscular de 1ml (25μl) de lecirelina, e os seus efeitos avaliados pela análise das concentrações séricas de estradiol, progesterona e corticosterona (0h, 6h, 24h, 48h e 96h pós-aplicação); pela verificação do desenvolvimento ovariano e ocorrência de ovulação através de exames ultrassonográficos (24h, 48h e 96h); pela observação de comportamento reprodutivo (0h, 48h e 96h) e pela postura de ovos. O tratamento foi repetido no mesmo animal a cada 21 dias e em cada tratamento realizado, 2 fêmeas do grupo controle eram submetidas às mesmas análises. Os resultados indicam que a aplicação de lecirelina foi capaz de induzir a ovulação em 77% dos tratamentos realizados, com postura de ovos normais em 50% das induções. Assim, o tratamento proposto foi considerado eficiente na indução da ovulação em fêmeas de avestruz desde que seja aplicado em fêmeas que apresentem ovário bem desenvolvido.

The lack of knowledge about the ostrich reproduction delays the development of the ostrich commercial breeding due to a low reproductive performance. The aim of this study was to evaluate the effect of the application of an analog of GnRH (Lecirelin - Gestran Plus®) for induction of ovulation in adult female ostriches. Initially, a group of 10 adult female ostriches were evaluated in order to determine their reproductive status, by measuring serum levels of estradiol and progesterone (blood sampling - 3 times a week), by the observation of reproductive behavior, by the evaluation of the ovarian development through ultrasound examination (repeated every 21 days) and by the egg laying data. After the end of the first phase, female ostriches were separated into a control group (n = 3) and a treated group (n = 6). Every week, two females from the treated group were selected to receive the treatment: an intramuscular application of 1 ml (25μl) of Lecirelin. The effect of the application was evaluated by the analysis of serum concentrations of estradiol, progesterone and corticosterone (0h, 6h, 24h, 48h and 96h post-application), by the verification of the ovarian development and the occurrence of ovulation through ultrasound examinations (24h, 48h and 96h), by the observation of…

Advisors/Committee Members: Guimarães, Marcelo Alcindo de Barros Vaz.

Subjects/Keywords: Análogo de GNRH; Avestruz; GnRH analog; Hormones; Hormônios; Ostrich; Reprodução; Reproduction; Ultrasound; Ultrassom

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, G. C. d. O. e. (2012). Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;

Chicago Manual of Style (16^th Edition):

Silva, Guilherme Costa de Oliveira e. “Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus).” 2012. Doctoral Dissertation, University of São Paulo. Accessed March 22, 2019. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;.

MLA Handbook (7^th Edition):

Silva, Guilherme Costa de Oliveira e. “Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus).” 2012. Web. 22 Mar 2019.

Vancouver:

Silva GCdOe. Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus). [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2019 Mar 22]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;.

Council of Science Editors:

Silva GCdOe. Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus). [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;

Universidade Federal de Viçosa

26. Guilherme Silva Moura. Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço.

Degree: 2008, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015

►

Este trabalho foi realizado com os seguintes objetivos: a) avaliar o efeito do hormônio liberador de gonadotropina (GnRH), administrado em diferentes momentos do manejo reprodutivo,… (more)

▼

Este trabalho foi realizado com os seguintes objetivos: a) avaliar o efeito do hormônio liberador de gonadotropina (GnRH), administrado em diferentes momentos do manejo reprodutivo, na taxa de prenhez e concentração sérica de P4 em gado de corte mestiço; e b) avaliar o efeito do hormônio liberador de gonadotropinas (GnRH), administrado 12 dias após IATF, sobre os níveis séricos de progesterona e, principalmente, na taxa de prenhez em vacas de corte mestiças. Para o primeiro objetivo, utilizaram-se 82 fêmeas bovinas mestiças (Bos taurus indicus x Bos taurus taurus), alocadas ao acaso, em três tratamentos: Tcontrole (n = 28): os animais foram observados para detecção de estros e inseminados após 8 a 12 horas; TGnRH0 (n = 27): similar ao Tcontrole, com administração de 25 μg de GnRH (Gestran Plus, Lecirelina) no momento da IA; e TGnRH0-12 (n = 27): similar ao Tcontrole, com administração de 25 μg de GnRH no momento da IA e no dia 12 após a IA. No dia 35, após IA, foi feito o diagnóstico de gestação por exames ultrassonográficos pela via transretal. Foi observado que 57,14% das vacas (16/28) no Tcontrole e no TGnRH0 e TGnRH12, 62,96% (17/27) ficaram gestantes após o primeiro serviço. Não foi observada diferença entre os animais dos tratamentos (P >0,05). Também não se observou diferença entre as concentrações de P4 séricas entre os animais dos tratamentos. Concluiu-se que a administração de análogo de GnRH no momento da IA ou 12 dias após a IA não melhorou o desempenho reprodutivo em vacas mestiças, nem a sintese de P4 pelo corpo lúteo. Para o segundo objetivo, foram utilizadas 59 fêmeas bovinas mestiças (Bos taurus indicus x Bos taurus taurus), alocadas ao acaso, em dois tratamentos: TBE (n = 30): no dia 0, inseriu-se o dispositivo intravaginal de progesterona (Primer) mais 2,0 mg de BE (Estrogin), im; no dia 8, retirou-se o PRIMER e aplicaram-se 300 UI de eCG (Novormon) e 0,15 mg de PGF2α (Prolise), im; no dia 9, aplicou-se 1 mg de BE, im, e realizou-se a IATF 48-56 horas após a retirada do PRIMER; e TBEGnRH12 (n = 29): o protocolo foi similar ao do TBE, porém com administração de 25 μg de GnRH (Gestran Plus, Lecirelina) no dia 12 após a IA. No dia 35, após IA, foi feito o diagnóstico de gestação por exames ultrassonográficos pela via transretal. Neste estudo foi observado que 53,33% das vacas (16/30) no TBE e 37,93% (11/29) no TBEGnRH12 ficaram gestantes após o primeiro serviço. Não foi observada diferença entre os animais dos tratamentos (P >0,05). Também não se observou diferença entre as concentrações de P4 entre os animais dos tratamentos (P >0,05). Concluiu-se que a administração do análogo de GnRH, lecirelina, no dia 12, após a IATF, não afetou as taxas de prenhez e nem a concentração de progesterona dos animais dos tratamentos, nos dias 0, 5, 12 e 20 após a IATF.

The experiment was done with the following objectives: a) to evaluate the effect of GnRH administered at different times of the reproductive management on the pregnancy rate and serum P4 concentration; and b) to evaluate the…

Advisors/Committee Members: Giovanni Ribeiro de Carvalho, José Domingos Guimarães, Eduardo Paulino da Costa, Elenice Andrade Moraes e Amorim, Ciro Alexandre Alves Torres.

Subjects/Keywords: GnRH; PRODUCAO ANIMAL; Bovino de corte; IATF; Progesterona; Beef cattle; GnRH; FTAI; Progesterone

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moura, G. S. (2008). Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moura, Guilherme Silva. “Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço.” 2008. Thesis, Universidade Federal de Viçosa. Accessed March 22, 2019. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moura, Guilherme Silva. “Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço.” 2008. Web. 22 Mar 2019.

Vancouver:

Moura GS. Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço. [Internet] [Thesis]. Universidade Federal de Viçosa; 2008. [cited 2019 Mar 22]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moura GS. Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço. [Thesis]. Universidade Federal de Viçosa; 2008. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Castillo Farfán, Juan Carlos. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.

Degree: 2018, TDX

URL: http://hdl.handle.net/10550/23452

► Human gonadotropin chorionic hormone (hCG) is widely used to induce final follicular maturation in in vitro fertilization cycles (IVF). However, its use is closely related… (more)

Subjects/Keywords: Agonista GnRH; antagonista GnRH; hCG; Síndrome de hiperestimulación ovárica; Facultat de Medicina; Ginecologia. Obstetricia

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Castillo Farfán, J. C. (2018). Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. (Thesis). TDX. Retrieved from http://hdl.handle.net/10550/23452

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Castillo Farfán, Juan Carlos. “Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.” 2018. Thesis, TDX. Accessed March 22, 2019. http://hdl.handle.net/10550/23452.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Castillo Farfán, Juan Carlos. “Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.” 2018. Web. 22 Mar 2019.

Vancouver:

Castillo Farfán JC. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. [Internet] [Thesis]. TDX; 2018. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10550/23452.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Castillo Farfán JC. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10550/23452

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Castillo Farfán, Juan Carlos. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.

Degree: 2018, TDX

URL: http://hdl.handle.net/10803/567845

► Human gonadotropin chorionic hormone (hCG) is widely used to induce final follicular maturation in in vitro fertilization cycles (IVF). However, its use is closely related… (more)

Subjects/Keywords: Agonista GnRH; antagonista GnRH; hCG; Síndrome de hiperestimulación ovárica; Facultat de Medicina; Ginecologia. Obstetricia

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Castillo Farfán, J. C. (2018). Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. (Thesis). TDX. Retrieved from http://hdl.handle.net/10803/567845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Castillo Farfán, Juan Carlos. “Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.” 2018. Thesis, TDX. Accessed March 22, 2019. http://hdl.handle.net/10803/567845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Castillo Farfán, Juan Carlos. “Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.” 2018. Web. 22 Mar 2019.

Vancouver:

Castillo Farfán JC. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. [Internet] [Thesis]. TDX; 2018. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10803/567845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Castillo Farfán JC. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. [Thesis]. TDX; 2018. Available from: http://hdl.handle.net/10803/567845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade de Lisboa

29. Madureira, Márcio André Gonçalves. Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy.

Degree: 2012, Universidade de Lisboa

URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709

►

Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2012

Recent advances in cancer treatment field allowed significant increase… (more)

▼

Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2012

Recent advances in cancer treatment field allowed significant increase in the survival rate of patients. However, the patients have commonly faced long-term adverse effects that severly affected the quality of life, specially concerning their fertility. It is established that radio- and chemotherapy treatments can cause a reduction of the ovarian reserve, resulting in a 40-60% rate of premature ovarian failure (POF) in women exposed to these treatments. In order to decrease POF risks, several fertility preservation options were developed: reduction of the exposure to gonadotoxic agents, gametes or embryos cryopreservation, oocyte donation, ovarian tissue cryopreservation and transplant, or a pharmacological protection of the ovaries during chemotherapy. While the last one appears as a less invasive and promising procedure, the studies and clinical trials continue to show inconsistent results, raising an almost 30-year discussion and controversy. The gonadotropin-releasing hormone (GnRH) is a hypothalamic hormone responsible for the releasing of gonadotropins. Due to its pulsatile fashion to induce FSH secretion, it was proposed that a continuous saturation of the receptor by synthetic GnRH analogues (GnRHa) could decrease the gonadotropins release and, therefore, could maintain the ovarian follicular pool at immature stages. Once it was suggested that initial stage follicles were less affected by alkylating agents, the women capacity to conceive could then be preserved. By a multiple approach study design, including histological, immunohistochemical, in vitro and in vivo assays in a mouse model, our group intended to better understand the potential preventive effect of GnRHa on the ovaries exposed to chemotherapy and to evaluate the efficiency of GnRH agonists (AGOs) and antagonists (ANTs) in this indication. Our results suggest, so far, that AGOs (triptorelin) and ANTs (cetrorelix) are not efficient to prevent the follicular depletion induced by a cyclophosphamide (Cy) treatment. Nevertheless, the fertility follow-up, until now, seems to show that the birth rate is not affected by neither Cy nor combined Cy-GnRHa treatment, suggesting that the mouse experimental model is not yet optimal.

Nos últimos anos, foram feitos enormes avanços no que diz respeito aos tratamentos oncológicos. Estes conseguiram aumentar significativamente as taxas de sobrevivência e o aumento da esperança média de vida dos doentes oncológicos. No entanto, este incremento nem sempre foi acompanhado por uma melhoria na qualidade de vida, nomeadamente na preservação da fertilidade das mulheres sujeitas a tratamentos radio- e quimioterapeuticos. Em média, após este tipo de tratamentos, 40-60% das mulheres são diagnosticadas com falência ovárica precoce (FOP), caracterizada essencialmente por uma diminuição acentuada no número de folículos pertencentes à reserva ovárica de mulheres ainda em idade-reprodutora, derivando…

Advisors/Committee Members: Demeestere, Isabelle, Rodrigues, Gabriela.

Subjects/Keywords: Fertilidade; Quimioterapia; Agonistas da GnRH; Antagonistas da GnRH; Teses de Mestrado - 2012

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Madureira, M. A. G. (2012). Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy. (Thesis). Universidade de Lisboa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Madureira, Márcio André Gonçalves. “Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy.” 2012. Thesis, Universidade de Lisboa. Accessed March 22, 2019. http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Madureira, Márcio André Gonçalves. “Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy.” 2012. Web. 22 Mar 2019.

Vancouver:

Madureira MAG. Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy. [Internet] [Thesis]. Universidade de Lisboa; 2012. [cited 2019 Mar 22]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Madureira MAG. Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy. [Thesis]. Universidade de Lisboa; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de València

30. Castillo Farfán, Juan Carlos. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.

Degree: Departament de Pediatria, Obstetrícia i Ginecologia, 2010, Universitat de València

URL: http://hdl.handle.net/10803/52180

► Human gonadotropin chorionic hormone (hCG) is widely used to induce final follicular maturation in in vitro fertilization cycles (IVF). However, its use is closely related… (more)

Subjects/Keywords: Agonista GnRH; antagonista GnRH; hCG; Síndrome de hiperestimulación ovárica; Facultat de Medicina; 618

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Castillo Farfán, J. C. (2010). Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. (Thesis). Universitat de València. Retrieved from http://hdl.handle.net/10803/52180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Castillo Farfán, Juan Carlos. “Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.” 2010. Thesis, Universitat de València. Accessed March 22, 2019. http://hdl.handle.net/10803/52180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Castillo Farfán, Juan Carlos. “Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea.” 2010. Web. 22 Mar 2019.

Vancouver:

Castillo Farfán JC. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. [Internet] [Thesis]. Universitat de València; 2010. [cited 2019 Mar 22]. Available from: http://hdl.handle.net/10803/52180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Castillo Farfán JC. Ciclos de fecundación in vitro y maduración final con agonista GNRH: Explorando dosis bajas de HCG para soporte de fase lutea. [Thesis]. Universitat de València; 2010. Available from: http://hdl.handle.net/10803/52180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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