subject:(GnRH)

1. Vosges, Mélanie. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.

Degree: Docteur es, Sciences de la vie, 2010, Université François-Rabelais de Tours

URL: http://www.theses.fr/2010TOUR4035

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A ce jour, les effets des perturbateurs endocriniens (PE) sur les circuits neuroendocrines contrôlant la fonction de reproduction ont fait l’objet de très peu de… (more)

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A ce jour, les effets des perturbateurs endocriniens (PE) sur les circuits neuroendocrines contrôlant la fonction de reproduction ont fait l’objet de très peu de travaux. Chez les vertébrés, l’élément majeur du contrôle central de la fonction de reproduction est la GnRH (Gonadotropin-Releasing Hormone). Le développement et l’activité des neurones à GnRH sont finement régulés, notamment par les hormones stéroïdes, ce qui les rend potentiellement sensibles aux PE. L’objectif de ce travail était d'étudier les effets neuroendocrines des xéno-œstrogènes chez le poisson zèbre (Danio rerio). Nous montrons que le 17α-éthinylestradiol (EE2) et le nonylphénol (NP) perturbent l'ontogenèse du système à GnRH au cours du développement précoce. De plus, nous démontrons que ces effets impliquent des récepteurs des œstrogènes. Parallèlement, nous mettons en évidence l’effet inducteur de l’EE2 et du NP sur l'expression de l’aromatase cérébrale, l’enzyme de synthèse des œstrogènes. L’ensemble de ces données souligne la nécessité de considérer les réseaux neuroendocrines comme des variables critiques et sensibles dans le domaine de la perturbation endocrinienne.

Until now, studies dedicated to the actions of endocrine disrupting chemicals (EDCs) on the reproductive axis have focused on the gonads and peripheral organs leaving virtually unexplored their actions on neuroendocrine circuits controlling reproduction. In vertebrates, gonadotropin-releasing hormone (GnRH) is the key factor controlling the activity of the reproductive axis. The development and functioning of GnRH neurons are finely tuned, notably by sex steroids, making these neurons potential targets of EDCs. The aim of this work was to explore the neuroendocrine effects of xenoestrogens in the zebrafish (Danio rerio). We show that 17α-ethinylestradiol (EE2) and nonylphenol (NP) disrupts the ontogeny of GnRH system during zebrafish early life stage. Moreover, we demonstrate that these effects involve functional estrogens receptors. In parallel, we report the inducing effects of EE2 and NP on the expression of brain aromatase protein, the enzyme responsible for estrogen biosynthesis. Altogether, these results highlight the need to consider neuroendocrine networks as critical and sensitive endpoints in the field of endocrine disruption.

Advisors/Committee Members: Combarnous, Yves (thesis director).

Subjects/Keywords: GnRH

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APA (6^th Edition):

Vosges, M. (2010). Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2010TOUR4035

Chicago Manual of Style (16^th Edition):

Vosges, Mélanie. “Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.” 2010. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 07, 2021. http://www.theses.fr/2010TOUR4035.

MLA Handbook (7^th Edition):

Vosges, Mélanie. “Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system.” 2010. Web. 07 Mar 2021.

Vancouver:

Vosges M. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2010. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2010TOUR4035.

Council of Science Editors:

Vosges M. Effets neuroendocrines des perturbateurs endocriniens chez le poisson zèbre (Danio rerio) : étude du système à GnRH. : Neuroendocrine effects of endocrine disruptors in zebrafish (danio rerio) : study of GnRH system. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2010. Available from: http://www.theses.fr/2010TOUR4035

Texas A&M University

2. De Souza Scarpa, Jose Oswaldo. Comparison of Follicular and Luteal Dynamics in Protocols Developed for Synchronization of Ovulation in Bos indicus-Influenced Beef Cows: The Role of Gonadotropin-Releasing Hormone at Treatment Onset.

Degree: MS, Physiology of Reproduction, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173684

► The 5-day Bee Synch + CIDR (Bee Synch) protocol for Bos indicus-influenced cows utilizes CIDR, GnRH (GnRH-1) and PGF on day 0 to eliminate mature… (more)

▼ The 5-day Bee Synch + CIDR (Bee Synch) protocol for Bos indicus-influenced cows utilizes CIDR, GnRH (GnRH-1) and PGF on day 0 to eliminate mature corpora lutea, with FTAI and GnRH (GnRH-2) at 66 hours after CIDR removal to yield pregnancy rates of ~ 50%. The objective was to test the hypothesis that GnRH-1 is not required to optimize follicle synchrony. Seventy-one cycling Brangus and Brahman × Hereford suckled cows were used in 2 replicates (35-36 per replicate). Cows were stratified by BW, BCS, and days postpartum, and assigned randomly in a 2 × 3 factorial arrangement involving 2 truncated (no FTAI or GnRH-2) versions of Bee Synch (BS-I and II) begun 3, 7 and 10 days post ovulation. Ovulation was pre-synchronized with PGF. Cows in BS-I received 100 µg GnRH i.m., 25 mg PGF i.m., and a CIDR on treatment onset. Cows in BS-II did not receive GnRH-1. On day 5, CIDRs were removed and all cows received 50 mg PGF i.m. Estrus was detected with Estrotect patches. Daily ultrasonography confirmed ovulation. Synchronized new follicular wave emergence (NFWE; days 1-4) was observed in 68.6 and 38.9% (BS-I vs. BS-II; P = 0.01) of cows and increased to 93.3 and 72.2%, respectively, if days 0-4 were considered. Size of the largest follicle at 66 hours (13.5 ± 0.47 mm) did not differ by treatment or day post ovulation. Interval from CIDR removal to ovulation was greater (P = 0.02) for BS-I (5.2 ± 0.2 days) than BS-II (4.4 ± 0.2 days) and greater (P < 0.0001) for day 3 (6.35 ± 0.3 days) than days 7 and 10 (4.31 ± 0.3 and 3.7 ± 0.3, respectively). Progesterone concentrations did not differ between treatments (P = 0.12). The LH peak at 120 minutes after GnRH-1 was higher (4.28 ± 0.71 ng/mL, P < 0.05) for day 3 than days 7 and 10. A greater (P = 0.06) proportion (15.5%) of cows in BS-II ovulated by 72 hours than in BSI. GnRH-1 reduced variation in day to NFWE and incidence of early ovulations. Advisors/Committee Members: Williams, Gary L (advisor), de Carvalho Cardoso, Rodolfo (advisor), Welsh, Jr, Thomas H (committee member), Looney, Charles R (committee member).

Subjects/Keywords: Bee Synch; GnRH

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APA (6^th Edition):

De Souza Scarpa, J. O. (2018). Comparison of Follicular and Luteal Dynamics in Protocols Developed for Synchronization of Ovulation in Bos indicus-Influenced Beef Cows: The Role of Gonadotropin-Releasing Hormone at Treatment Onset. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173684

Chicago Manual of Style (16^th Edition):

De Souza Scarpa, Jose Oswaldo. “Comparison of Follicular and Luteal Dynamics in Protocols Developed for Synchronization of Ovulation in Bos indicus-Influenced Beef Cows: The Role of Gonadotropin-Releasing Hormone at Treatment Onset.” 2018. Masters Thesis, Texas A&M University. Accessed March 07, 2021. http://hdl.handle.net/1969.1/173684.

MLA Handbook (7^th Edition):

De Souza Scarpa, Jose Oswaldo. “Comparison of Follicular and Luteal Dynamics in Protocols Developed for Synchronization of Ovulation in Bos indicus-Influenced Beef Cows: The Role of Gonadotropin-Releasing Hormone at Treatment Onset.” 2018. Web. 07 Mar 2021.

Vancouver:

De Souza Scarpa JO. Comparison of Follicular and Luteal Dynamics in Protocols Developed for Synchronization of Ovulation in Bos indicus-Influenced Beef Cows: The Role of Gonadotropin-Releasing Hormone at Treatment Onset. [Internet] [Masters thesis]. Texas A&M University; 2018. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1969.1/173684.

Council of Science Editors:

De Souza Scarpa JO. Comparison of Follicular and Luteal Dynamics in Protocols Developed for Synchronization of Ovulation in Bos indicus-Influenced Beef Cows: The Role of Gonadotropin-Releasing Hormone at Treatment Onset. [Masters Thesis]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173684

University of Otago

3. Cheong, Rachel Yanghui. Effects of estradiol on gonadotropin-releasing hormone neurons .

Degree: 2012, University of Otago

URL: http://hdl.handle.net/10523/2570

► The gonadotropin-releasing hormone (GnRH) neurons, located within the hypothalamus of the mammalian brain, have been established as the key output cells mediating all influences that… (more)

▼ The gonadotropin-releasing hormone (GnRH) neurons, located within the hypothalamus of the mammalian brain, have been established as the key output cells mediating all influences that control fertility, conveyed by the central nervous system. Gonadotropins (luteinizing hormone; LH and follicle stimulating hormone; FSH), secreted by the pituitary in response to hypothalamic signals from GnRH neurons, stimulate the release of gonadal steroids from the ovaries. The gonadal steroid, estradiol (E2), functions as a diverse and indispensible hormone capable of modulating multiple facets of neuronal functioning. Circulating E2 provides neuroendocrine feedback to the hypothalamus and pituitary, through negative and positive feedback mechanisms depending on the hormonal milieu across the ovulatory cycle. E2 exerts a negative feedback response on GnRH neurons to keep basal gonadotropin secretion low and switches over to positive feedback to generate the pre-ovulatory gonadotropin surge, required for ovulation. Although GnRH neurons express estrogen receptor β (ERβ), the other receptor subtype, ERα, has been described as the main receptor mediating positive feedback. However, a role for both ERα and ERβ in estrogen negative feedback remains likely. The mechanisms underlying negative feedback actions of estrogen upon the GnRH neuronal network are unclear and likely to require different mechanisms of E2 action including direct actions through ERβ-expressing GnRH neurons or indirectly through ERα-expressing afferent inputs to GnRH neurons. E2 is known to act through the non-classical pathway to rapidly activate intracellular signaling cascades to alter cellular function and it is believed that these rapid non-classical E2 actions are likely contributors involved in mediating estrogen negative feedback in mice. The purpose of this study was to investigate the mechanisms mediating estrogen negative feedback on the GnRH neuronal system. The specific intracellular signaling pathways mediating rapid non-classical E2 actions were first addressed employing both in vivo and in vitro acute brain slice approaches involving dual-label fluorescence and peroxidase-based immunohistochemistry in a negative feedback model. This model involved ovariectomizing mice and testing the ability of the acute E2 treatment to rapidly induce the phosphorylation of the cyclic-AMP response element binding protein (CREB) and the upstream molecule, extracellular signal-regulated kinase 1 and 2 (ERK1/2). The administration of E2 to adult female mice in vivo resulted in the rapid activation of ERK1/2 in GnRH neurons in a time-dependent manner and in vitro studies using pharmacological antagonists showed that ERK1/2 was essential for E2-induced CREB phosphorylation in GnRH neurons. Upstream to this, protein kinase A (PKA) and calcium/calmodulin dependent protein kinase type II (CaMKII), but not protein kinase C were found to be necessary for E2-induced phosphorylation of ERK1/2 and CREB. Interestingly, this rapid E2 signaling cascade in GnRH neurons was found to… Advisors/Committee Members: Ábrahám, István (advisor).

Subjects/Keywords: Estradiol; GnRH; negativefeedback; non-classical

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APA (6^th Edition):

Cheong, R. Y. (2012). Effects of estradiol on gonadotropin-releasing hormone neurons . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/2570

Chicago Manual of Style (16^th Edition):

Cheong, Rachel Yanghui. “Effects of estradiol on gonadotropin-releasing hormone neurons .” 2012. Doctoral Dissertation, University of Otago. Accessed March 07, 2021. http://hdl.handle.net/10523/2570.

MLA Handbook (7^th Edition):

Cheong, Rachel Yanghui. “Effects of estradiol on gonadotropin-releasing hormone neurons .” 2012. Web. 07 Mar 2021.

Vancouver:

Cheong RY. Effects of estradiol on gonadotropin-releasing hormone neurons . [Internet] [Doctoral dissertation]. University of Otago; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10523/2570.

Council of Science Editors:

Cheong RY. Effects of estradiol on gonadotropin-releasing hormone neurons . [Doctoral Dissertation]. University of Otago; 2012. Available from: http://hdl.handle.net/10523/2570

Universiteit Utrecht

4. Athmer, P.L.T.M. Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana).

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/186901

► Elephant populations in Southern Africa have been increasing in number following their protective status as endangered species by CITES in 1989, to the extent that… (more)

▼ Elephant populations in Southern Africa have been increasing in number following their protective status as endangered species by CITES in 1989, to the extent that elephant populations have to be regulated. Elephant population control can be done in two basic ways: decreasing their number (i.e. culling or relocation) or preventing further increase by reduction of their reproductive rate (i.e. contraception). Culling elephants is an effective but controversial method due to public disapproval, while translocation is a less suitable method due to various practical problems. Contraceptive methods should ideally meet certain prerequisites based on efficacy, minimal animal handling, reversibility, safety for use in pregnant animals, minimal health side effects, no passage through the food chain, minimal effects on behaviour and low costs. Contraceptive methods as surgical sterilization, mechanical and hormonal contraception do not meet all of these prerequisites. Immunocontraception has become a point of interest with the antigens zona pellucida (ZP) and gonadotrophin-releasing hormone (GnRH) most intensively studied. GnRH is a neuropeptide produced by the hypothalamus and plays a crucial role in hypothalamic-pituitary-ovarian axis involved in ovulation. By vaccinating against GnRH, antibodies are thought to bind to GnRH, thereby blocking an important step in the ovulation process. The ultimate purpose of GnRH vaccination is to induce infertility by returning treated animals to a (temporary) pharmalogical pre-pubertal state. The eventual aim of this study was to test a commercial GnRH vaccine, Improvac®, for its effect on female African elephants. In this current pilot study we measured the progestagen and glucocorticoids metabolite levels in the faeces of two African elephant cows during an entire oestrus cycle (20 weeks) prior to GnRH vaccination. The faecal concentrations were estimated using Enzyme Immuno Assays (EIA) for 5α pregnane-3-ol-20-one (progesterone metabolite) and 3α,11-Oxo-cortisol (glucocorticoid metabolite). The aim by doing so was to provide a baseline for faecal progestagen and glucocorticoid concentrations in cycling non-pregnant African elephant cows and investigate correlations between reproductive status (progestagen) and stress (glucocorticoids) using a non-invasive sampling method. - 3 - Although the number of samples analysed in this study was limited, it did demonstrate that faecal progesterone concentrations could be used to demonstrate luteal activity, making it of considerable use for assessing the effects of GnRH vaccination. Faecal glucocorticoids levels did not appear to be significantly influenced by stage of cycle. A complete database of glucocorticoids levels during the long term studies could be helpful to determine whether GnRH vaccination is detrimental to welfare. Advisors/Committee Members: Stout, Prof TAE.

Subjects/Keywords: Diergeneeskunde; elephant, GnRH, progesterone, glucocorticoid

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APA (6^th Edition):

Athmer, P. L. T. M. (2008). Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana). (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/186901

Chicago Manual of Style (16^th Edition):

Athmer, P L T M. “Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana).” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed March 07, 2021. http://dspace.library.uu.nl:8080/handle/1874/186901.

MLA Handbook (7^th Edition):

Athmer, P L T M. “Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana).” 2008. Web. 07 Mar 2021.

Vancouver:

Athmer PLTM. Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana). [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2021 Mar 07]. Available from: http://dspace.library.uu.nl:8080/handle/1874/186901.

Council of Science Editors:

Athmer PLTM. Correlation between progesterone and glucocorticoid metabolite concentrations in the faeces of free roaming African elephants (Loxodonta africana africana). [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/186901

Texas A&M University

5. O'Neil, Meaghan Marie. The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares.

Degree: MS, Physiology of Reproduction, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/169637

► Continuous infusion with native gonadotropin-releasing hormone (GnRH) restores follicular growth and ovulation in approximately 85% of winter anovulatory mares. However, the potential role of estradiol-17β… (more)

▼ Continuous infusion with native gonadotropin-releasing hormone (GnRH) restores follicular growth and ovulation in approximately 85% of winter anovulatory mares. However, the potential role of estradiol-17β (E2) in enhancing pituitary responsiveness to GnRH during this transitional process is unclear. Moreover, the contribution of follicle-stimulating hormone (FSH) to the overall response has not been studied. Objectives herein were to test the hypotheses that (1) continuous treatment with either a low or high dose of E2 enhances pituitary responsiveness to continuous infusion of native GnRH, and (2) the pattern of FSH secretion mimics that of LH. Twenty-four winter anovulatory mares were stratified by body weight and body condition score, and assigned randomly to 1 of 4 treatments (n = 6/treatment) in early February: 1) Control; Silastic® subcutaneous (S.C.) sham pump, 2) GnRH; native GnRH in saline via 14-day Alzet® osmotic pump (100 μg/h, S.C.), 3) GnRH + High E2; GnRH as in 2 plus Compudose® E2 implant, (25.7-mg, S.C.), and 4) GnRH + Low E2; GnRH as in 2 and 3 plus 1/4-length E2 implant (6.425-mg, S.C.). Plasma concentrations of LH, FSH, E2, estrone sulfate, and progesterone were measured in daily blood samples. Mares developing 35-mm follicles were induced to ovulate with human chorionic gonadotropin (hCG). Mares not ovulating within 14 days received a second 14-day GnRH pump. Although acute increases in uterine edema observed within 48 hours indicated implant functionality, plasma E2 and estrone sulfate were increased primarily in associationwith a large, Continuous infusion with native gonadotropin-releasing hormone (GnRH) restores follicular growth and ovulation in approximately 85% of winter anovulatory mares. However, the potential role of estradiol-17β (E2) in enhancing pituitary responsiveness to GnRH during this transitional process is unclear. Moreover, the contribution of follicle-stimulating hormone (FSH) to the overall response has not been studied. Objectives herein were to test the hypotheses that (1) continuous treatment with either a low or high dose of E2 enhances pituitary responsiveness to continuous infusion of native GnRH, and (2) the pattern of FSH secretion mimics that of LH. Twenty-four winter anovulatory mares were stratified by body weight and body condition score, and assigned randomly to 1 of 4 treatments (n = 6/treatment) in early February: 1) Control; Silastic® subcutaneous (S.C.) sham pump, 2) GnRH; native GnRH in saline via 14-day Alzet® osmotic pump (100 μg/h, S.C.), 3) GnRH + High E2; GnRH as in 2 plus Compudose® E2 implant, (25.7-mg, S.C.), and 4) GnRH + Low E2; GnRH as in 2 and 3 plus 1/4-length E2 implant (6.425-mg, S.C.). Plasma concentrations of LH, FSH, E2, estrone sulfate, and progesterone were measured in daily blood samples. Mares developing 35-mm follicles were induced to ovulate with human chorionic gonadotropin (hCG). Mares not ovulating within 14 days received a second 14-day GnRH pump. Although acute increases in uterine edema observed within 48 hours indicated… Advisors/Committee Members: Williams, Gary L (advisor), Cardoso, Rodolfo C (committee member), Welsh, Thomas H (committee member), Hinrichs, Katrin (committee member).

Subjects/Keywords: Mare; winter anovulatory; GnRH; E2

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APA (6^th Edition):

O'Neil, M. M. (2017). The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/169637

Chicago Manual of Style (16^th Edition):

O'Neil, Meaghan Marie. “The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares.” 2017. Masters Thesis, Texas A&M University. Accessed March 07, 2021. http://hdl.handle.net/1969.1/169637.

MLA Handbook (7^th Edition):

O'Neil, Meaghan Marie. “The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares.” 2017. Web. 07 Mar 2021.

Vancouver:

O'Neil MM. The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1969.1/169637.

Council of Science Editors:

O'Neil MM. The Effects of Estradiol-17beta in Modulating Pituitary-Ovarian Responsiveness to Continuous Infusion of Gonadotropin-Releasing Hormone in Winter Anovulatory Mares. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/169637

University of Waikato

6. Jeffries , Kent Peter James. Assessment of key reproductive markers after hormonal induction of spawning, using gonadotrophin-releasing hormone in female yellow belly flounder (Rhombosolea leporine):.

Degree: 2019, University of Waikato

URL: http://hdl.handle.net/10289/13487

► Yellow belly flounder (YBF) (Rhombosolea leporina) are of interest to the New Zealand aquaculture industry as a novel culture species. This is due to their… (more)

▼ Yellow belly flounder (YBF) (Rhombosolea leporina) are of interest to the New Zealand aquaculture industry as a novel culture species. This is due to their high commercial value and low trophic feeding level. However, when held in captive settings, YBF are observed to undergo reproductive failure. GnRHa has been used as a spawning inducing agent within many cultured fish species. Flounder gonadotrophin levels were traced after induction and oocyte development was histologically assessed. At pituitary level it was seen that the GnRHa induction resulted in increased follicle stimulating hormone (FSH) levels. Luteinising hormone (LH) was seen to be unaffected. However, neither of these changes were significant P>0.05. These weak results were most likely caused by seasonality. Oocyte development was seen to follow a similar trend to other flounder and flatfish species, when oocyte size, development stages and features were tracked using histological analysis. Relationships between Gonadosomatic index (GSI) and gonadotrophins FSH and LH displayed weak correlations (P>0.05). This again could be linked to seasonal variability in temperature and photoperiod. The presence of large amounts of atretic oocytes observed in the gonads indicated that the ovary had already spawned for the season before initial capture, or that oocytes had been aborted. A likely cause of this would be captivity -induced stress, or fish having previously spawned before capture. All fish that ovulated were part of the GnRHa treated group. Fish were strip-spawned, fertilised and embryonic development was tracked. Incubation temperatures were 17 and 19°C. It was established that of the two temperatures, 17°C was optimum. A larger percentage of these embryos survived from fertilisation to hatch, which took approximately 68 hours. Bacterial infection was seen to be a problem in eggs incubated at the higher 19°C temperature. Egg quality was assessed by looking at the 8 cell blastomere stage, and grading charts were made for reference. these assessed blastomere size, shape, symmetry and cohesion. It is suggested that future research efforts focus on seasonal variations of gonadotrophin levels and gonadal development, in order to gain clearer understanding of these seasonal effects. Effects of cortisol on the reproductive axis would also be a beneficial research area. This would provide insight into how stress affects sex steroid production and the maturation of the gonad and oocytes in YBF. Advisors/Committee Members: Muncaster, Simon (advisor).

Subjects/Keywords: Yellow belly flounder; GnRH

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APA (6^th Edition):

Jeffries , K. P. J. (2019). Assessment of key reproductive markers after hormonal induction of spawning, using gonadotrophin-releasing hormone in female yellow belly flounder (Rhombosolea leporine):. (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/13487

Chicago Manual of Style (16^th Edition):

Jeffries , Kent Peter James. “Assessment of key reproductive markers after hormonal induction of spawning, using gonadotrophin-releasing hormone in female yellow belly flounder (Rhombosolea leporine):. ” 2019. Masters Thesis, University of Waikato. Accessed March 07, 2021. http://hdl.handle.net/10289/13487.

MLA Handbook (7^th Edition):

Jeffries , Kent Peter James. “Assessment of key reproductive markers after hormonal induction of spawning, using gonadotrophin-releasing hormone in female yellow belly flounder (Rhombosolea leporine):. ” 2019. Web. 07 Mar 2021.

Vancouver:

Jeffries KPJ. Assessment of key reproductive markers after hormonal induction of spawning, using gonadotrophin-releasing hormone in female yellow belly flounder (Rhombosolea leporine):. [Internet] [Masters thesis]. University of Waikato; 2019. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10289/13487.

Council of Science Editors:

Jeffries KPJ. Assessment of key reproductive markers after hormonal induction of spawning, using gonadotrophin-releasing hormone in female yellow belly flounder (Rhombosolea leporine):. [Masters Thesis]. University of Waikato; 2019. Available from: http://hdl.handle.net/10289/13487

7. Andrade, Luís Pedro Mota Pinto de. Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows.

Degree: 2013, Instituto Politécnico de Castelo Branco

URL: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817

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Reproductive efficiency in beef cows is limited by the length of the postpartum anoestrous period wich in turn is controlled by pituitary gonadotrophins and gonadal… (more)

Subjects/Keywords: Beef cattle; Reproduction; GnRH; LH

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APA (6^th Edition):

Andrade, L. P. M. P. d. (2013). Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows. (Thesis). Instituto Politécnico de Castelo Branco. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andrade, Luís Pedro Mota Pinto de. “Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows.” 2013. Thesis, Instituto Politécnico de Castelo Branco. Accessed March 07, 2021. https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andrade, Luís Pedro Mota Pinto de. “Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows.” 2013. Web. 07 Mar 2021.

Vancouver:

Andrade LPMPd. Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows. [Internet] [Thesis]. Instituto Politécnico de Castelo Branco; 2013. [cited 2021 Mar 07]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andrade LPMPd. Effects of gonadothrophin-releasing hormone and bovine somatotrophin on hormone profiles and ovarian function in postpartum beef cows. [Thesis]. Instituto Politécnico de Castelo Branco; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:repositorio.ipcb.pt:10400.11/1817

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat de Valencia

8. Cruz Quiroga, Ramiro de la. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis .

Degree: 2015, Universitat de Valencia

URL: http://hdl.handle.net/10550/50501

► La aromatasa juega un papel fundamental en el establecimiento de la calidad ovocitaria, la cual puede estar alterada en las mujeres infértiles con endometriosis. Por… (more)

▼ La aromatasa juega un papel fundamental en el establecimiento de la calidad ovocitaria, la cual puede estar alterada en las mujeres infértiles con endometriosis. Por otro lado, la administración prolongada de agonistas de GnRH previo a la fecundación in vitro en estas pacientes, ha demostrado que aumentaba la tasa de gestación aproximadamente cuatro veces. En este estudio se ha comparado la expresión del gen CYP19A1 ( que codifica a la aromatasa) en las células de la granulosa mural (CGM) y en las células de cúmulo (CC), los niveles de estradiol, testosterona y androstenediona en el líquido folicular, la calidad ovocitaria y embrionaria, y la tasa de fecundación de pacientes con endometriosis pre-tratadas con agonistas de GnRH durante 3 meses frente a placebo previo la realización de la FIV. Las células de la granulosa han sido aisladas y la expresión del gen CYP19A1 ha sido cuantificada mediante reacción en cadena de la polimerasa en tiempo real. Las determinaciones hormonales en el liquido folicular se han hecho mediante inmunoensayo, concretamente mediante quimioluminiscencia. La calidad ovocitaria y embrionaria se ha determinado según parámetros morfológicos. En cuanto a los resultados, no se han detectado diferencias significativas en la expresión del gen de la aromatasa en las dos poblaciones celulares estudiadas (CGM y CC) entre ambos grupos ( CGM: grupo control 1,23 ± 3,19 y grupo de estudio 1,44 ± 1,85; CC: grupo control 2,61 ± 3,96 y grupo de estudio 2,15 ± 2,99). Tampoco se han encontrado diferencias estadísticamente significativas al analizar los resultados teniendo en cuenta la unilateralidad de la lesión endometriósica (ovario afectado con el folículo ipsilateral), la bilateralidad, por muestras pareadas y según el grado de endometriosis. Las concentraciones de las hormonas esteroides en el líquido folicular han sido más bajas en el grupo de estudio, siendo el nivel de testosterona estadísticamente significativa ( 9,38 ± 7,94 ng/ml vs 3,10 ± 2,44 ng/ml, control vs estudio respectivamente, p=0,00). En el análisis detallado de resultados la tendencia de los esteroides foliculares ha sido la misma en los casos de unilateralidad y bilateralidad (siendo la testosterona significativamente más baja en el grupo de estudio vs placebo). En el análisis por muestras pareadas, no ha habido diferencias entre ambos grupos. En el análisis según grado de endometriosis, la significancia estadística de las testosterona se ha evidenciado sólo en las endometriosis mínimas o leves ( control: 9,49 ±6,66 ng/ml vs estudio : 3,59 ± 2,56 ng/ml, p=0,00). No se han encontrado diferencias estadísticas en los parámetros de calidad ovocitaria ni embrionaria al comparar ambos grupos. La tasa de fecundación ha sido más baja y significativa en el grupo de estudio que había recibido el pre-tratamiento con agonistas (control: 77,23 ± 20,44 vs estudio: 58,92 ± 28,25, p =0,03). Se ha comparado también si existían diferencias en la expresión de las CGM frente a las CC en ambos grupos, no encontrando… Advisors/Committee Members: Monzó Miralles, Ana (advisor).

Subjects/Keywords: aromatasa; endometriosis; agonistas GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cruz Quiroga, R. d. l. (2015). Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/50501

Chicago Manual of Style (16^th Edition):

Cruz Quiroga, Ramiro de la. “Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis .” 2015. Doctoral Dissertation, Universitat de Valencia. Accessed March 07, 2021. http://hdl.handle.net/10550/50501.

MLA Handbook (7^th Edition):

Cruz Quiroga, Ramiro de la. “Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis .” 2015. Web. 07 Mar 2021.

Vancouver:

Cruz Quiroga Rdl. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2015. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10550/50501.

Council of Science Editors:

Cruz Quiroga Rdl. Efecto de los análogos de la GnRH sobre la expresión de la aromatasa en células de la granulosa de pacientes con endometriosis . [Doctoral Dissertation]. Universitat de Valencia; 2015. Available from: http://hdl.handle.net/10550/50501

Universitat de Valencia

9. Rodríguez Tárrega, Elisabet. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro .

Degree: 2016, Universitat de Valencia

URL: http://hdl.handle.net/10550/55362

► Introducción La endometriosis se define como la presencia de tejido endometrial fuera de la cavidad uterina, que induce una reacción inflamatoria local. La fisiopatología de… (more)

▼ Introducción La endometriosis se define como la presencia de tejido endometrial fuera de la cavidad uterina, que induce una reacción inflamatoria local. La fisiopatología de esta enfermedad no está completamente establecida, aunque parece que existen factores hormonales, genéticos e inmunológicos implicados. Su prevalencia exacta es desconocida, pero se estima que hasta el 10% de las pacientes en edad reproductiva y el 50% de pacientes con esterilidad la padecen. Esta enfermedad se asocia con dolor y esterilidad. En estas pacientes, las adherencias pueden obliterar las trompas de Falopio e impedir la liberación del ovocito; además, la reserva ovárica podría estar disminuida por la presencia de endometriomas y/o su tratamiento quirúrgico, mientras que la respuesta ovárica a la estimulación con gonadotrofinas puede estar disminuida. Sin embargo, estas no son las únicas causas de esterilidad en las pacientes con endometriosis, habiéndose propuesto otros mecanismos. Estos incluyen un ambiente folicular y peritoneal alterado con un aumento de citoquinas, que afecta a la foliculogénesis y origina una disminución de la calidad ovocitaria y embrionaria.. Así mismo, se ha observado una disfunción en las células de la granulosa y un efecto tóxico del estado inflamatorio presente en estas pacientes, mediado por macrófagos, interleuquina-8 y citoquinas pro-inflamatorias secretadas en los implantes endometriósicos. Por último, la función espermática y las tasas de fecundación e implantación están también afectadas por este ambiente inflamatorio. La mejor opción terapéutica para pacientes estériles con endometriosis continua siendo controvertida, pero lo que está claro es que muchas de ellas necesitarán realizar técnicas de reproducción asistida (TRA). Diversos estudios han mostrado peores resultados reproductivos tras TRA en pacientes con endometriosis que en pacientes con otras causas de esterilidad. Por este motivo, distintas investigaciones se han llevado a cabo para encontrar tratamientos adyuvantes que mejoren estos resultados reproductivos en pacientes con endometriosis. En este sentido, los agonistas de GnRH (GnRHa) podrían aumentar la tasa de gestación en estas mujeres. Estos fármacos causan una inhibición del eje hipofisario-gonadal que disminuye el estímulo hormonal para el crecimiento de las lesiones endometriósicas reduciendo la reacción inflamatoria presente en las pacientes con endometriosis. Un pre-tratamiento con agonistas de GnRH durante 3 meses previo a fecundación in vitro (FIV) en pacientes estériles con endometriosis podría mejorar las tasas de gestación clínica al disminuir el efecto perjudicial de las citoquinas y del estrés oxidativo. Hipótesis La administración de un análogo de GnRH durante los tres meses previos a la realización de una FIV en pacientes con endometriosis/endometriomas puede mejorar la respuesta ovárica a la estimulación y las tasas de implantación y de gestación clínica. Objetivos Objetivo principal Evaluar el efecto de la administración de un análogo de GnRH… Advisors/Committee Members: Monzó Miralles, Ana (advisor).

Subjects/Keywords: endometriosis; fiv; agonistas gnrh

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodríguez Tárrega, E. (2016). Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/55362

Chicago Manual of Style (16^th Edition):

Rodríguez Tárrega, Elisabet. “Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro .” 2016. Doctoral Dissertation, Universitat de Valencia. Accessed March 07, 2021. http://hdl.handle.net/10550/55362.

MLA Handbook (7^th Edition):

Rodríguez Tárrega, Elisabet. “Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro .” 2016. Web. 07 Mar 2021.

Vancouver:

Rodríguez Tárrega E. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10550/55362.

Council of Science Editors:

Rodríguez Tárrega E. Estudio comparativo, randomizado, ciego, del efecto del pre-tratamiento con análogos de GnRH frente a placebo en pacientes estériles diagnosticadas de endometriosis que realizan tratamiento de fecundación in vitro . [Doctoral Dissertation]. Universitat de Valencia; 2016. Available from: http://hdl.handle.net/10550/55362

Louisiana State University

10. Johnson, Carrie Ann. Endocrine and reproductive responses to implants of deslorein acetate in horses.

Degree: PhD, Animal Sciences, 2002, Louisiana State University

URL: etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218

► Four experiments were performed to study the effects of the gonadotropin releasing hormone (GnRH) analog, deslorelin acetate (Ovuplant™), on endocrine and reproductive characteristics in mares.… (more)

▼ Four experiments were performed to study the effects of the gonadotropin releasing hormone (GnRH) analog, deslorelin acetate (Ovuplant™), on endocrine and reproductive characteristics in mares. The first experiment tested whether anecdotal field reports of Ovuplant causing extended interovulatory intervals would be detectable under controlled, experimental conditions. The use of Ovuplant to hasten ovulation in 13 mares, compared to 12 controls, increased (P < 0.05) the interovulatory interval by 6.2 d and suppressed (P < 0.05) plasma concentrations of both luteinizing hormone (LH) and follicle stimulating hormone (FSH) for approximately 11 d. Two mares receiving Ovuplant did not return to estrus within 30 d. In the second experiment, 10 control mares and 10 mares induced to ovulate with Ovuplant were administered GnRH (50 μg) on d 1, 4, 7, and 10 after ovulation. Again, treated mares had a longer (4.4 d, P < 0.05) interovulatory interval and suppressed LH and FSH concentrations in daily plasma samples. The gonadotropin response to GnRH was lower (P < 0.05) in the deslorelin mares on d 1, 4, and 7, indicating a lack of pituitary responsiveness. In the third experiment, 9 stallions and 12 steroid-treated geldings were used to determine if males were potential models for studying the deslorelin-induced gonadotropin suppression. In both cases, treatment with Ovuplant caused an initial rise in both gonadotropins followed by suppression for about 14 d. In the last experiment, 21 mares were used to determine if multiple doses of deslorelin would cause complete ovarian shutdown. Mares received either sham injections, three Ovuplant implants on the first day, or one implant per day for 3 d (n = 7 per group). Treatment with multiple deslorelin implants increased (P < 0.05) the interovulatory interval by 14.8 d and suppressed LH and FSH concentrations for approximately 25 d, however no mares exhibited complete ovarian shutdown. In conclusion, deslorelin acetate implants in horses in the form of Ovuplant induce short-term increases in LH and FSH secretion followed by long-term suppression of these concentrations and an insensitivity of the pituitary to GnRH. In a small percentage of mares, long-term ovarian shutdown is a possibility.

Subjects/Keywords: horses; gnrh

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Johnson, C. A. (2002). Endocrine and reproductive responses to implants of deslorein acetate in horses. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218

Chicago Manual of Style (16^th Edition):

Johnson, Carrie Ann. “Endocrine and reproductive responses to implants of deslorein acetate in horses.” 2002. Doctoral Dissertation, Louisiana State University. Accessed March 07, 2021. etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218.

MLA Handbook (7^th Edition):

Johnson, Carrie Ann. “Endocrine and reproductive responses to implants of deslorein acetate in horses.” 2002. Web. 07 Mar 2021.

Vancouver:

Johnson CA. Endocrine and reproductive responses to implants of deslorein acetate in horses. [Internet] [Doctoral dissertation]. Louisiana State University; 2002. [cited 2021 Mar 07]. Available from: etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218.

Council of Science Editors:

Johnson CA. Endocrine and reproductive responses to implants of deslorein acetate in horses. [Doctoral Dissertation]. Louisiana State University; 2002. Available from: etd-0711102-111720 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3218

11. Márlon de Vasconcelos Azevedo. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.

Degree: 2012, Universidade Federal Rural de Pernambuco

URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

► Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência… (more)

▼ Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência reprodutiva e resultados econômicos em um programa comercial de transferência de embriões, no agreste pernambucano. No experimento 01 foram utilizadas quinze éguas doadoras de embrião sendo dez da Raça Quarto de Milha e cinco da raça Mangalarga Machador, com idade entre oito e vinte anos. O grupo 1 (G1) iniciou-se com o monitoramento do ciclo estral, inseminação atrificial durante o estro e colheita de embriões no oitavo dia pós ovulação. O Grupo 2 (G2) constituido pelas mesmas éguas do G1, tendo início com a aplicação de 7,5 mg de dinoprost-trometamina, o monitoramento dos ovários através do ultarassom, duas vezes ao dia até a detecção do maior folículo com diâmetro de 23 a 25 mm e o segundo >18 mm, momento inicial do tratamento superovulatório, com 100 μg de acetato de deslorelina, com intervalo de 12 h, até quando o maior folículo atingisse o diâmetro de 33 a 35 mm, quando foi realizado a indução da ovulação atravez da aplicação de 1000 μg de acetato de deslorelina IM associado a 1000 UI de hCG IV. No experimento 02 foram utilizadas sete éguas doadoras, sendo quatro da Raça Quarto de Milha e três da raça Mangalarga Machador, com idade entre oito a vinte anos. Os grupos G1 e G2 são iguais ao experimento 01, sendo acrescido do G3 que se iniciou após a reaplicação de 7,5 mg de dinoprosttrometamina após a colheita do G2, 48 h após é iniciado o tratamento com 100 μg (1ml) de acetato de deslorelina, com intervalo de 12h, até que o maior folículo atingisse 341 mm de diâmetro ou 6 dias de aplicação, quando foi feita a indução de ovulação com o mesmo protocolo do G2 . No experimento 01 o percentual de éguas com múltiplas ovulações no G1 e G2 foi de (2/15) 13,33% e de (13/15) 86,66%, respectivamente (P<0,005). Também foi observado um incremento no número de embriões recuperados, sendo no G1 apenas nove, enquanto no G2 foram de dezesseis embriões (P<0,005), com uma média de recuperação de 0,60,48 no G1 e de 1,0660,497 no G2 (P>0,005); e o percentual de recuperação embrionária foi de 60% G1 e de 80% no G2 (P<0,005). No experimento 02 o percentual de éguas com múltiplas ovulações no G1, G2 e G3 foi de 14,28% (1/7), 100,00% (7/7) e 0% (0/7) respectivamente (P<0,005). Foi observado um incremento no número de embriões recuperados sendo no G1 três, G2 seis e G3 zero (P<0,005), com uma média de recuperação embrionária de 0,43 0,53 no G1, de 0,86 0,38 no G2 e 0 G3 (P>0,005); com um percentual de recuperação embrionária de 43% G1, de 85,71% no G2 e 0% no G3 (P<0,005). Os resultados obtidos nestes experimentos permitem concluir que a administração de GnRH (deslorelina) em baixa dose em éguas doadoras de embriões, interferiu positivamente no crescimento do folículo dominante e do segundo folículo, aumentando a incidência de ovulações múltiplas. Incrementando a taxa de recuperação embrionária por ciclo, melhorando a eficiência reprodutiva das doadoras… Advisors/Committee Members: Paulo Fernandes de Lima, Marcos Antonio Lemos de Oliveira, Adriana Wanderley Taveiros, Claudio Coutinho Bartolomeu.

Subjects/Keywords: Reprodução; Biotecnologias; Equinos; GnRH; MEDICINA VETERINARIA; Biotechnology; Reproduction; Equine; GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Azevedo, M. d. V. (2012). Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 07, 2021. http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Web. 07 Mar 2021.

Vancouver:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2012. [cited 2021 Mar 07]. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Thesis]. Universidade Federal Rural de Pernambuco; 2012. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Márlon de Vasconcelos Azevedo. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.

Degree: 2012, Universidade Federal Rural de Pernambuco

URL: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

► Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência… (more)

▼ Objetivou-se com este trabalho induzir ovulações múltiplas em éguas utilizando baixas doses de GnRH (deslorelina) em tempo fixo e determinar seu impacto sobre a eficiência reprodutiva e resultados econômicos em um programa comercial de transferência de embriões, no agreste pernambucano. No experimento 01 foram utilizadas quinze éguas doadoras de embrião sendo dez da Raça Quarto de Milha e cinco da raça Mangalarga Machador, com idade entre oito e vinte anos. O grupo 1 (G1) iniciou-se com o monitoramento do ciclo estral, inseminação atrificial durante o estro e colheita de embriões no oitavo dia pós ovulação. O Grupo 2 (G2) constituido pelas mesmas éguas do G1, tendo início com a aplicação de 7,5 mg de dinoprost-trometamina, o monitoramento dos ovários através do ultarassom, duas vezes ao dia até a detecção do maior folículo com diâmetro de 23 a 25 mm e o segundo >18 mm, momento inicial do tratamento superovulatório, com 100 μg de acetato de deslorelina, com intervalo de 12 h, até quando o maior folículo atingisse o diâmetro de 33 a 35 mm, quando foi realizado a indução da ovulação atravez da aplicação de 1000 μg de acetato de deslorelina IM associado a 1000 UI de hCG IV. No experimento 02 foram utilizadas sete éguas doadoras, sendo quatro da Raça Quarto de Milha e três da raça Mangalarga Machador, com idade entre oito a vinte anos. Os grupos G1 e G2 são iguais ao experimento 01, sendo acrescido do G3 que se iniciou após a reaplicação de 7,5 mg de dinoprosttrometamina após a colheita do G2, 48 h após é iniciado o tratamento com 100 μg (1ml) de acetato de deslorelina, com intervalo de 12h, até que o maior folículo atingisse 341 mm de diâmetro ou 6 dias de aplicação, quando foi feita a indução de ovulação com o mesmo protocolo do G2 . No experimento 01 o percentual de éguas com múltiplas ovulações no G1 e G2 foi de (2/15) 13,33% e de (13/15) 86,66%, respectivamente (P<0,005). Também foi observado um incremento no número de embriões recuperados, sendo no G1 apenas nove, enquanto no G2 foram de dezesseis embriões (P<0,005), com uma média de recuperação de 0,60,48 no G1 e de 1,0660,497 no G2 (P>0,005); e o percentual de recuperação embrionária foi de 60% G1 e de 80% no G2 (P<0,005). No experimento 02 o percentual de éguas com múltiplas ovulações no G1, G2 e G3 foi de 14,28% (1/7), 100,00% (7/7) e 0% (0/7) respectivamente (P<0,005). Foi observado um incremento no número de embriões recuperados sendo no G1 três, G2 seis e G3 zero (P<0,005), com uma média de recuperação embrionária de 0,43 0,53 no G1, de 0,86 0,38 no G2 e 0 G3 (P>0,005); com um percentual de recuperação embrionária de 43% G1, de 85,71% no G2 e 0% no G3 (P<0,005). Os resultados obtidos nestes experimentos permitem concluir que a administração de GnRH (deslorelina) em baixa dose em éguas doadoras de embriões, interferiu positivamente no crescimento do folículo dominante e do segundo folículo, aumentando a incidência de ovulações múltiplas. Incrementando a taxa de recuperação embrionária por ciclo, melhorando a eficiência reprodutiva das doadoras… Advisors/Committee Members: Paulo Fernandes de Lima, Marcos Antonio Lemos de Oliveira, Adriana Wanderley Taveiros, Claudio Coutinho Bartolomeu.

Subjects/Keywords: Reprodução; Biotecnologias; Equinos; GnRH; MEDICINA VETERINARIA; Biotechnology; Reproduction; Equine; GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Azevedo, M. d. V. (2012). Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. (Thesis). Universidade Federal Rural de Pernambuco. Retrieved from http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Thesis, Universidade Federal Rural de Pernambuco. Accessed March 07, 2021. http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Azevedo, Márlon de Vasconcelos. “Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas.” 2012. Web. 07 Mar 2021.

Vancouver:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Internet] [Thesis]. Universidade Federal Rural de Pernambuco; 2012. [cited 2021 Mar 07]. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Azevedo MdV. Indução de múltiplas ovulações utilizando baixa dose de GnRH (deslorelina) em éguas. [Thesis]. Universidade Federal Rural de Pernambuco; 2012. Available from: http://200.17.137.108/tde_busca/arquivo.php?codArquivo=1466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Junqueira, Flávia Raquel Rosa. Uso do análogo do GnRH para diagnóstico de puberdade precoce.

Degree: Mestrado, Ginecologia e Obstetrícia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;

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Introdução - A puberdade precoce verdadeira ou dependente de GnRH apresenta importante morbidade: a baixa estatura, conseqüência da rápida progressão da idade óssea, além das… (more)

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Introdução - A puberdade precoce verdadeira ou dependente de GnRH apresenta importante morbidade: a baixa estatura, conseqüência da rápida progressão da idade óssea, além das seqüelas psico-emocionais do desenvolvimento sexual secundário precoce. Daí a importância da realização de um diagnóstico precoce e preciso, a fim de que a terapêutica adequada seja instituída o quanto antes. O uso do análogo do GnRH (aGnRH) em teste diagnóstico vem sendo utilizado com este objetivo. Neste estudo avaliou-se os valores de corte para o diagnóstico de puberdade precoce verdadeira, usando-se o teste do aGnRH. Material e métodos - Estudo prospectivo, com 44 meninas, com desenvolvimento dos caracteres sexuais secundários antes dos 8 anos de idade, atendidas no Ambulatório de Ginecologia Infanto-Puberal do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Realizou-se, em todos os casos, o teste do aGnRH, que consistiu na coleta de amostra sanguínea basal para dosagem de FSH e LH, seguida da aplicação subcutânea de 500µg de acetato de leuprolida (Lupron®). Novas amostras sanguíneas foram realizadas após 3 horas, para dosagem de FSH e LH, e após 24 horas da aplicação, para dosagem de estradiol Compararam-se os níveis de LH e FSH basais, de 3 horas e a relação LH/FSH obtida, além do estradiol de 24h, com a evolução clínica das pacientes. Este foi o padrão ouro utilizado para análise do teste, sendo que, após 6 meses, as pacientes foram divididas em 2 grupos: puberdade progressiva (puberdade precoce verdadeira) e não-progressiva. Para análise estatística, utilizou-se curvas ROC, estabelecendo-se sensibilidade, especificidade e melhor nível de corte para o diagnóstico de puberdade precoce verdadeira, para os diferentes critérios analisados. Além disso, avaliou-se a concordância entre os diversos tipos de análise do teste, através do coeficiente kappa. Resultados - O LH de 3 horas apresentou valor de corte > 4,5 mUI/mL, sensibilidade 59,1% e especificidade 86,4%, com área sobre a curva de 0,723. O valor de kappa foi de 0,45, com concordância de 0,73. O estradiol de 24 horas apresentou valor de corte > 40,6 pg/mL, sensibilidade 70% e especificidade 73,7%, com área sobre a curva de 0,703. O valor de kappa foi de 0,436, com concordância de 0,718. Dentre todos os critérios analisados, o melhor deles foi a relação LH/FSH de 3 horas, com valor de corte > 0,14, sensibilidade 72,7% e especificidade 77,3%, com área sobre a curva de 0,771. O valor de kappa foi de 0,5, com concordância de 0,75. Conclusões - Em nossa avaliação, a relação LH/FSH de 3 horas foi superior ao valor de LH de 3 horas ou estradiol de 24 horas, que haviam sidos os melhores critérios diagnósticos no trabalho pioneiro na utilização deste teste.

Introduction - True or GnRH-dependent precocious puberty involves important morbidity such as short stature due to the rapid progression of bone age, as well as psycho-emotional sequels of precocious secondary sexual development. Thus, it is important to make an early and precise diagnosis…

Advisors/Committee Members: Reis, Rosana Maria dos.

Subjects/Keywords: análogo do GnRH; diagnosis; diagnóstico; GnRH analogue; Precocious puberty; Puberdade precoce

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Junqueira, F. R. R. (2007). Uso do análogo do GnRH para diagnóstico de puberdade precoce. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;

Chicago Manual of Style (16^th Edition):

Junqueira, Flávia Raquel Rosa. “Uso do análogo do GnRH para diagnóstico de puberdade precoce.” 2007. Masters Thesis, University of São Paulo. Accessed March 07, 2021. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;.

MLA Handbook (7^th Edition):

Junqueira, Flávia Raquel Rosa. “Uso do análogo do GnRH para diagnóstico de puberdade precoce.” 2007. Web. 07 Mar 2021.

Vancouver:

Junqueira FRR. Uso do análogo do GnRH para diagnóstico de puberdade precoce. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Mar 07]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;.

Council of Science Editors:

Junqueira FRR. Uso do análogo do GnRH para diagnóstico de puberdade precoce. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-21062008-112920/ ;

Universidade do Rio Grande do Sul

14. Montenegro, Ivan Sereno. Avaliação da estimulação ovariana com uso de análogos do gnrh.

Degree: 2012, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/56618

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Introdução: Novos medicamentos trouxeram um considerável aumento na chance de gravidez no momento em que a estimulação ovariana controlada permitiu aumentar o número de oócitos… (more)

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Introdução: Novos medicamentos trouxeram um considerável aumento na chance de gravidez no momento em que a estimulação ovariana controlada permitiu aumentar o número de oócitos a serem recuperados e, consequentemente, um maior número de embriões para serem transferidos. Os análogos de hormônio liberador de gonadotrofina, em associação com gonadotrofinas, trouxeram a solução para um dos problemas da estimulação ovariana controlada: o pico precoce de hormônio luteinizante, que prejudicava a coleta de oócitos em torno de 20% dos casos. Rotineiramente, usamos o protocolo com agonista, mas a possibilidade do uso de antagonistas resulta em maior facilidade de manuseio pelo usuário, envolve um único menstrual ciclo e dispensa orientação para uso de drogas, no final do ciclo anterior. Assim, buscamos avaliar o uso dos dois protocolos e analisar seus resultados. Objetivos: Análise e comparação de dados entre dois protocolos de indução (longo com agonista e flexível com antagonista) em pacientes submetidas a técnicas de reprodução assistida na SEGIR – Serviço de Ecografica, Genética e Reprodução Assistida – Porto Alegre. Métodos: Estudo transversal comparando os resultados intermediários com o uso de dois diferentes protocolos de estimulação ovariana com de agonista versus antagonista do hormônio liberador de gonadotrofina para técnicas de reprodução assistida. A análise estatística dos dados recuperados (idade, índice de massa corpórea, número de oócitos recuperados, número de oócitos fertilizados, número de oócitos clivados, dose total de FSH utilizada e ocorrência de síndrome do hiperestímulo ovariano) foi realizada através de teste t de Student para dados paramétricos e análise de covariância para as variáveis dependentes, calculados com o programa SPSS 16.0. Resultados: Um total de 50 pacientes preencheram os critérios para inclusão no estudo entre janeiro e março no ano de 2010, sendo 25 em cada grupo. Houve diferença estatística apenas na idade média entre os grupos (p=0,031). Não houve diferença estatística para os demais dados analizados (índice de massa corpórea, número de oócitos recuperados, número de oócitos fertilizados, número de oócitos clivados e dose de FSH utilizada) entre os grupos. Não houve casos de síndrome do hiperestímulo ovariano. Conclusão: Ambos protocolos os são iguais em termos de resultados. O agonista tem vantagens sobre o agendamento do procedimento, mas leva muito tempo para começar a estimulação e tem a possibilidade de iniciar a medicação em uma paciente grávida. Somado a isso, temos a possibilidade de ter síndrome do hiperestímulo ovariano como complicação. No grupo antagonista, está claro a maior facilidade de uso da medicação e o início mais rápido da estimulação ovariana.

Background: New medications have brought a considerable increase in the chance of pregnancy at the time that controlled ovarian stimulation allowed an increase in the number of oocytes to be recruited and, consequently, a greater number of embryos to be transferred. The gonadotropin-releasing hormone analogues, in…

Advisors/Committee Members: Passos, Eduardo Pandolfi.

Subjects/Keywords: IVF; Técnicas de reprodução assistida; Infertilidade; Outcome/controlled; Ovarian stimulation/GnRH; Indução da ovulação; Analogue/GnRH; Agonist/GnRH; Antagonist

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Montenegro, I. S. (2012). Avaliação da estimulação ovariana com uso de análogos do gnrh. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/56618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Montenegro, Ivan Sereno. “Avaliação da estimulação ovariana com uso de análogos do gnrh.” 2012. Thesis, Universidade do Rio Grande do Sul. Accessed March 07, 2021. http://hdl.handle.net/10183/56618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Montenegro, Ivan Sereno. “Avaliação da estimulação ovariana com uso de análogos do gnrh.” 2012. Web. 07 Mar 2021.

Vancouver:

Montenegro IS. Avaliação da estimulação ovariana com uso de análogos do gnrh. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10183/56618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Montenegro IS. Avaliação da estimulação ovariana com uso de análogos do gnrh. [Thesis]. Universidade do Rio Grande do Sul; 2012. Available from: http://hdl.handle.net/10183/56618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Benz, Nathalie. Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title.

Degree: Docteur es, Biologie-santé, 2013, Brest

URL: http://www.theses.fr/2013BRES0021

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Introduction : La mucoviscidose est une maladie génétique autosomale récessive causée par des mutations dans le gène CFTR (cystic fibrosis transmembrane conductance regulator). Ce dernier… (more)

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Introduction : La mucoviscidose est une maladie génétique autosomale récessive causée par des mutations dans le gène CFTR (cystic fibrosis transmembrane conductance regulator). Ce dernier code un canal chlorure AMPc-dépendant localisé dans la membrane apicale des cellules épithéliales, dont l’activité est régulée par de nombreuses interactions protéine-protéine. Dans le cadre de la recherche de nouveaux partenaires du CFTR, une interaction directe entre le canal (sauvage et muté F508del) etl’annexine A5 (AnxA5) a été mise en évidence dans notre laboratoire. Des stratégies de sur et de sousexpression nous ont également permis d’établir un lien fonctionnel entre les deux protéines. En effet, nos travaux montrent que les sécrétions ioniques dépendantes du CFTR sont corrélées au niveau d’expression intracellulaire de l’AnxA5. Par ailleurs, une élévation des courants médiés par le CFTR ainsi qu’une augmentation de la quantité de canaux dans la membrane plasmique sont observées suite à la surexpression de l’AnxA5 dans des cellules exprimant le CFTR muté F508del.But de l’étude : Au vu de ces observations, l’AnxA5 apparaît comme une cible potentielle pour la correction de certains défauts engendrés par la mutation F508del. Une piste thérapeutique pourrait être l’identification de composés capables d’augmenter son expression dans des cellules épithéliales exprimant le mutant F508del de la protéine CFTR. Considérant les informations fournies par la littérature, notre choix s’est porté sur la GnRH (gonadotropin-releasing hormone), molécule utilisée en thérapeutique humaine depuis plus de 25 ans. Ainsi, nous avons évalué l’effet de la GnRH sur la modulation de l’expression de l’AnxA5 et sur le transport ionique dépendant du CFTR dans nos différents modèles d’étude Résultats : Outre la présence du récepteur de la GnRH dans nos modèles cellulaires, nous montrons également que l’expression de l’AnxA5 y est augmentée dès 60 minutes de traitement avec l’hormone (1 nM). De plus, comparativement à des cellules non stimulées, des cellules prétraitées avec la GnRH présentent une hausse significative des sorties actives d’iodure, corrélant avec une augmentation de la quantité de CFTR à la surface cellulaire. Ces observations ont été faites dans les modèles exprimant le CFTR muté F508del ainsi que dans ceux exprimant le CFTR sauvage. Conclusion : Dans nos modèles et selon nos conditions de stimulation, un traitement avec la GnRH augmente l’expression intracellulaire de l’AnxA5 et conduit à une élévation des sécrétions ioniques médiées par le canal CFTR. Néanmoins, au vu de la multitude de voies de signalisation susceptibles d’être activées et de gènes pouvant être régulés suite à la liaison de la GnRH sur son récepteur, l’effet observé sur l’AnxA5 ne représente probablement pas le seul évènement cellulaire à l’origine de l’impact positif enregistré sur l’activité du canal CFTR.

Background: Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene,…

Advisors/Committee Members: Férec, Claude (thesis director).

Subjects/Keywords: CFTR; Mutation F508del; Transport ionique; AnxA5; GnRH; Récepteur de la GnR; CFTR; F508del mutation; Ion transport; AnxA5; GnRH; GnRH recepto

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benz, N. (2013). Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title. (Doctoral Dissertation). Brest. Retrieved from http://www.theses.fr/2013BRES0021

Chicago Manual of Style (16^th Edition):

Benz, Nathalie. “Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title.” 2013. Doctoral Dissertation, Brest. Accessed March 07, 2021. http://www.theses.fr/2013BRES0021.

MLA Handbook (7^th Edition):

Benz, Nathalie. “Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title.” 2013. Web. 07 Mar 2021.

Vancouver:

Benz N. Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title. [Internet] [Doctoral dissertation]. Brest; 2013. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2013BRES0021.

Council of Science Editors:

Benz N. Stimulation de cellules épithéliales bronchiques humaines par la GnRH : effet sur le transport ionique médié par le CFTR : No title. [Doctoral Dissertation]. Brest; 2013. Available from: http://www.theses.fr/2013BRES0021

University of Otago

16. de Croft, Simon. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse .

Degree: University of Otago

URL: http://hdl.handle.net/10523/5611

► The gonadotropin-releasing hormone (GnRH) neurons are the principal neurons in a network within the hypothalamus that controls fertility in all mammals. GnRH neuron output is… (more)

▼ The gonadotropin-releasing hormone (GnRH) neurons are the principal neurons in a network within the hypothalamus that controls fertility in all mammals. GnRH neuron output is influenced by a wide range of neurotransmitters. One recently-discovered and essential neurotransmitter in this network is known as kisspeptin. Kisspeptin neurons exist in two distinct populations; in the rostral periventricular nucleus of the third ventricle (RP3V) and in the arcuate nucleus (ARN). Based upon anatomical evidence, the RP3V kisspeptin neurons are hypothesized to be important for the positive feedback mechanism of estrogen that drives the preovulatory GnRH surge. Also, anatomical evidence has led to the suggestion that the ARN kisspeptin neuronal population is involved in the negative feedback mechanism that homeostatically suppresses GnRH release and also is important to drive pulsatile GnRH secretion. In my PhD, I have performed experiments to gain functional insight into the roles of these two kisspeptin neuronal populations. To identify kisspeptin neurons, I have used a knock-in kisspeptin-GFP mouse line, in which only kisspeptin neurons should appear fluorescent. I used immunohistochemistry to show that kisspeptin neurons can be identified in vitro using GFP fluorescence. I have then used patch-clamp electrophysiology techniques to describe the spontaneous firing rate of kisspeptin neurons and described this across the estrous cycle. I demonstrate that the spontaneous electrical activity is not strongly subject to fluctuating gonadal steroid levels. Unexpectantly, I have described a male-dominant sex difference in spontaneous firing rate of ARN kisspeptin neurons. Additionally, I show that RP3V kisspeptin neurons are not strongly activated at the time of the GnRH surge when measured in an acute brain slice. ARN kisspeptin neurons are thought to form an interconnected network, and utilize coexpressed neuropeptides to generate a pulsatile output that is important for generating the pulsatile pattern of GnRH release from the hypothalamus. I have used cell-attached and whole-cell electrophysiological recording techniques to show that neurokinin B and dynorphin potently modulate ARN kisspeptin neuronal output in an opposing manner, and described the receptors that mediate these effects. Following this, I have used whole-cell electrophysiological recording techniques to test for functional connections between ARN kisspeptin neurons. Whereas no evidence was found for monosynaptic glutamatergic synaptic connections, some evidence was found for neuropeptidergic neurotransmission between ARN kisspeptin neurons. These results provide electrophysiological data to inform and update theories of kisspeptin neuronal function which are built largely on anatomical data. Advisors/Committee Members: Herbison, Allan (advisor).

Subjects/Keywords: Kisspeptin; Hypothalamus; GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

de Croft, S. (n.d.). Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/5611

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

de Croft, Simon. “Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse .” Doctoral Dissertation, University of Otago. Accessed March 07, 2021. http://hdl.handle.net/10523/5611.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

de Croft, Simon. “Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse .” Web. 07 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

de Croft S. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse . [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10523/5611.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

de Croft S. Electrophysiological Characteristics of Kisspeptin Neurons in the Hypothalamus of the Mouse . [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/5611

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Texas A&M University

17. Korthanke, Curtis M. Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare.

Degree: MS, Physiology of Reproduction, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/156795

► Seasonal reproduction is highly correlated with circulating concentrations of luteinizing hormone (LH) in mares and low peripheral concentrations of LH serve as the primary basis… (more)

▼ Seasonal reproduction is highly correlated with circulating concentrations of luteinizing hormone (LH) in mares and low peripheral concentrations of LH serve as the primary basis for ovarian quiescence during the non-breeding season. Four experiments were conducted to investigate the effects and underlying relationships of 1-adamantanecarbonyl-RF-NH2 (RF9) and estradiol in the regulation of LH secretion. During the luteal phase of the estrous cycle (Experiment 1), mares were treated with two bolus intravenous injections of RF9 (0.2 and 0.4 mg/kg, respectively) within a 1-h interval to determine effects on secretion of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Dose-dependent increases (P < 0.05) in both FSH and LH concentrations in response to RF9 were observed. Due to an inability to validate the GnRH radioimmunoassay, changes in secretion of GnRH could not be determined. In Experiment 2, the effect of estradiol-17β (estradiol) pretreatment on responsiveness to RF9 was examined in winter anovulatory mares. Mares received a single intramuscular injection of corn oil of 5 mg estradiol in corn oil followed by a bolus injection of RF9 18 h later. Estradiol pretreatment increased (P < 0.05) peak concentrations of LH in response to RF9. The half-life of RF9 in the circulation of the mare following peripheral administration was determined in Experiment 3 using liquid chromatography and mass spectrometry. Intravenously-injected RF9 was rapidly removed from the mare’s circulatory system; exhibiting a half-life of approximately 40 min. Finally, the role of estradiol and its interaction with GnRH in regulating secretion of LH in anovulatory mares during increasing photoperiod was examined. In February, mares were assigned to 1 of 4 groups and served as controls (corn oil injections) or received 1 of 3 treatments: daily i.m. injections of estradiol in corn oil for 14 d, continuous subcutaneous treatment with native GnRH for 14 d at a rate of 100 µg/h, or the estradiol and GnRH treatments in combination. Estradiol alone had no effect on secretion of LH but markedly enhanced (P < 0.0002) responsiveness to GnRH. Due to its short half-life, RF9 may not be useful therapeutically; however, estradiol enhances responsiveness to GnRH and may be useful in treatments designed to accelerate vernal transition. Advisors/Committee Members: Williams, Gary L (advisor), Brinsko, Steven P (committee member), Welsh, Jr., Thomas H (committee member).

Subjects/Keywords: Mare; Anovulatory; Seasonality; Estradiol; RF9; GnRH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Korthanke, C. M. (2016). Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156795

Chicago Manual of Style (16^th Edition):

Korthanke, Curtis M. “Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare.” 2016. Masters Thesis, Texas A&M University. Accessed March 07, 2021. http://hdl.handle.net/1969.1/156795.

MLA Handbook (7^th Edition):

Korthanke, Curtis M. “Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare.” 2016. Web. 07 Mar 2021.

Vancouver:

Korthanke CM. Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1969.1/156795.

Council of Science Editors:

Korthanke CM. Roles of Estradiol and a Putative Neuropeptide FF Receptor Antagonist, RF9 in Regulating Gonadotropin Secretion in the Cyclic and Seasonally Anovulatory Mare. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/156795

Angelo State University

18. Boenig, Michael Wade. The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers.

Degree: MS, Animal Science, 2012, Angelo State University

URL: http://hdl.handle.net/2346.1/30026

► The objective of this study was to determine if a pre synchronization gonadotropin releasing hormone (GnRH) injection would increase conception percentages in postpartum anestrous angus… (more)

Subjects/Keywords: gonadotropin; Angus; GnRH; conception; anestrous angus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boenig, M. W. (2012). The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers. (Masters Thesis). Angelo State University. Retrieved from http://hdl.handle.net/2346.1/30026

Chicago Manual of Style (16^th Edition):

Boenig, Michael Wade. “The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers.” 2012. Masters Thesis, Angelo State University. Accessed March 07, 2021. http://hdl.handle.net/2346.1/30026.

MLA Handbook (7^th Edition):

Boenig, Michael Wade. “The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers.” 2012. Web. 07 Mar 2021.

Vancouver:

Boenig MW. The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers. [Internet] [Masters thesis]. Angelo State University; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2346.1/30026.

Council of Science Editors:

Boenig MW. The effects of gonadotropin releasing hormone on conception in postpartum anestrus angus beef cows and heifers. [Masters Thesis]. Angelo State University; 2012. Available from: http://hdl.handle.net/2346.1/30026

19. Crossard, Elodie. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.

Degree: Docteur es, Sciences de la vie, 2011, Université François-Rabelais de Tours

URL: http://www.theses.fr/2011TOUR4026

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Le kisspeptine (kp) est un peptide, dérivé du gène kiss-1, jouant un rôle majeur dans le contrôle central de la fonction de reproduction en régulant… (more)

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Le kisspeptine (kp) est un peptide, dérivé du gène kiss-1, jouant un rôle majeur dans le contrôle central de la fonction de reproduction en régulant la sécrétion du GnRH chez l’adulte mais également au cours du développement. Les neurones exprimant kiss-1 sont situés dans la région rostrale périventriculaire du 3ème ventricule (RP3V) et le noyau arqué (ARC). L’expression de kiss-1 est hautement régulée par les stéroides sexuels, positivement dans RP3V et négativement dans ARC. Ces deux populations de neurones à kp semblent avoir des rôles différents. Les neurones à kp du RP3V seraient impliqués dans la genèse du pic préovulatoire et ceux de l’ARC dans la régulation de la sécrétion pulsatile de GnRH.L’objectif de la thèse était de déterminer la période de neurogenèse des neurones à kp ainsi que les variations de l’expression de kiss-1 et de kp dans ces deux régions au cours des différentes phases du développement chez le rat mâle et femelle.Nos résultats ont permis de cibler les périodes clés de l’ontogenèse des neurones à kp en montrant 1) que les neurones à kp de l’ARC naissent sur une période étendue à partir du jour embryonnaire (E)12,5; 2) l’existence d’une sous- expression péri-natale du kp dans l’ARC indépendante du sexe; 3) la mise en place, en période néonatale, de différences sexuelles dans les niveaux d’expression et la distribution neuroanatomique du kp; 4) l’existence de régulations péri-pubertaires de kp, dépendantes du sexe et de la région ; 5) la présence de fibres à kp dans des régions hypothalamiques suggère un rôle de kp au-delà de la fonction de reproduction.

Kisspeptin (kp) is a neuropeptide, derived from the kiss-1 gene, which plays a key role in the central control of reproduction by regulating GnRH secretion in adult but also during development. Cells which express kiss-1 are localized in two distincts hypothalamic regions: the rostral peri-ventricular third ventricule area (RP3V) and the arcuate nucleus (ARC). Kiss-1 expression is highly regulated by sex steroids: positively in the RP3V and negatively in the ARC. RP3V kp neurons have been implicated in the pre-ovulatory GnRH surge whereas ARC kp neurons may predominantly act on GnRH secretion pulsatility. The aim of this PhD work was to determine the neurogenesis period of kp neurons and changes of kiss-1 and kp expression in both regions during different stages of development in rats. Our results highlight key periods of kp neurons ontogenesis and show that: 1) ARC kp neurons are born during an extended embryonic neurogenesis period starting at embryonic day (E) 12,5; 2) a sex independent down-regulation of kp occurs during peri-natal period; 3) sex difference in the expression level and neuroanatomique distribution of kp establishes during neo-natal period; 4) kp was regulated during peri-pubertal period in sex and region dependant manner; 5) kp-ir fibers are detected throughout the septo-hypothalamic continuum suggesting that kp could be implicated in other functions than reproductive function.

Advisors/Committee Members: Duittoz, Anne (thesis director), Franceschini-Laurent, Isabelle (thesis director).

Subjects/Keywords: GnRH; Kisspeptin; Rat; Neurogenesis; Sex differentiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Crossard, E. (2011). Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2011TOUR4026

Chicago Manual of Style (16^th Edition):

Crossard, Elodie. “Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.” 2011. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 07, 2021. http://www.theses.fr/2011TOUR4026.

MLA Handbook (7^th Edition):

Crossard, Elodie. “Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood.” 2011. Web. 07 Mar 2021.

Vancouver:

Crossard E. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2011. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2011TOUR4026.

Council of Science Editors:

Crossard E. Ontogenèse des neurones à kisspeptine chez le rat : neurogénèse et cartographie spatio-temporelle de kisspeptine de l'embryogénèse à l'âge adulte : Ontogenesis of kisspeptin neurons in rat : neurogenesis and spatio-temporal cartographie of kisspeptin neurone from embryogenesis to adulthood. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2011. Available from: http://www.theses.fr/2011TOUR4026

University of Colorado

20. Ramelli, Sarah Leanne. Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons.

Degree: MS, Integrative Physiology, 2014, University of Colorado

URL: https://scholar.colorado.edu/iphy_gradetds/29

► Fibroblast growth factor receptor 1 (Fgfr1) gene mutations can cause deficiencies in gonadotropin-releasing hormone (GnRH) production in humans. GnRH deficiencies cause Kallmann syndrome and… (more)

Subjects/Keywords: Fgfr1; GnRH; reproductive impairment; Endocrinology; Physiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramelli, S. L. (2014). Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons. (Masters Thesis). University of Colorado. Retrieved from https://scholar.colorado.edu/iphy_gradetds/29

Chicago Manual of Style (16^th Edition):

Ramelli, Sarah Leanne. “Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons.” 2014. Masters Thesis, University of Colorado. Accessed March 07, 2021. https://scholar.colorado.edu/iphy_gradetds/29.

MLA Handbook (7^th Edition):

Ramelli, Sarah Leanne. “Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons.” 2014. Web. 07 Mar 2021.

Vancouver:

Ramelli SL. Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons. [Internet] [Masters thesis]. University of Colorado; 2014. [cited 2021 Mar 07]. Available from: https://scholar.colorado.edu/iphy_gradetds/29.

Council of Science Editors:

Ramelli SL. Reproductive Phenotype of Transgenic Mouse with Conditional Deletion of Fibroblast Growth Factor Receptor 1 in GnRH Neurons. [Masters Thesis]. University of Colorado; 2014. Available from: https://scholar.colorado.edu/iphy_gradetds/29

Duquesne University

21. Wack, Corina. Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders.

Degree: PhD, Biological Sciences, 2011, Duquesne University

URL: https://dsc.duq.edu/etd/1321

► Pheromones are chemosensory cues released by an individual to cause a behavioral or physiological change in a conspecific. These changes can range from increasing a… (more)

▼ Pheromones are chemosensory cues released by an individual to cause a behavioral or physiological change in a conspecific. These changes can range from increasing a female's receptivity to altering hormone secretions in the body. The red-legged salamander (Plethodon shermani) is an emerging non-mammalian model for understanding the evolution of chemical communication due to their well-characterized pheromones. Plethodontid salamanders secrete pheromones from their submandibular gland, called mental gland pheromones. Previous studies showed that mental gland pheromones increased receptivity in females during courtship and also increased corticosterone (CORT) concentrations in males. CORT is a metabolic hormone that mediates vertebrate stress responses. To further understand the neuroendocrine mechanisms involved in behavioral and physiological responses to pheromones, I conducted several experiments. First, I investigated whether pheromones altered levels of two neuromodulators in the brain, gonadotropin-releasing hormone (GnRH) and arginine vasotocin (AVT) in P. shermani. GnRH is both a neuromodulator and hormone, and has strong effects on reproductive behavior. Additionally, AVT has broad behavioral effects in vertebrates, particularly in newts. I found that application of mental gland pheromones altered the number of GnRH-immunoreactive neurons, but had no effect on the number of AVT-immunoreactive neurons. Second, I examined the effects of AVT and AVP antagonist on courtship and mating in dusky salamanders (Desmognathus spp.). There was no effect of AVT or antagonist on reproductive behaviors in dusky salamanders. Finally, I investigated the role of increased CORT concentrations in male P. shermani. I first validated a non-invasive method to transdermally deliver CORT through a dermal patch. I then used dermal patches to exogenously elevate plasma CORT and examine the effects of CORT on behavior (activity and chemoinvestigation) and metabolism. CORT had no effect on behavior, but increased metabolic rates in male red-legged salamanders. Together these studies provide insight into the mechanisms by which pheromones work to alter behaviors and physiological functions in vertebrates. Advisors/Committee Members: Sarah K. Woodley, Philip E. Auron, Richard P. Elinson, Timothy Smith.

Subjects/Keywords: Corticosterone; GnRH; Metabolism; Salamander; Stress; Vasotocin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wack, C. (2011). Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders. (Doctoral Dissertation). Duquesne University. Retrieved from https://dsc.duq.edu/etd/1321

Chicago Manual of Style (16^th Edition):

Wack, Corina. “Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders.” 2011. Doctoral Dissertation, Duquesne University. Accessed March 07, 2021. https://dsc.duq.edu/etd/1321.

MLA Handbook (7^th Edition):

Wack, Corina. “Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders.” 2011. Web. 07 Mar 2021.

Vancouver:

Wack C. Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders. [Internet] [Doctoral dissertation]. Duquesne University; 2011. [cited 2021 Mar 07]. Available from: https://dsc.duq.edu/etd/1321.

Council of Science Editors:

Wack C. Neuroendocrine Mechanisms Mediating Pheromonal Modulation of Behavior in Terrestrial Salamanders. [Doctoral Dissertation]. Duquesne University; 2011. Available from: https://dsc.duq.edu/etd/1321

22. Silva, Guilherme Costa de Oliveira e. Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus).

Degree: PhD, Reprodução Animal, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;

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A falta de conhecimento sobre a reprodução do avestruz dificulta o desenvolvimento da sua criação comercial devido a uma baixa eficiência reprodutiva. O objetivo deste… (more)

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A falta de conhecimento sobre a reprodução do avestruz dificulta o desenvolvimento da sua criação comercial devido a uma baixa eficiência reprodutiva. O objetivo deste estudo foi avaliar a ação da aplicação de um análogo de GnRH (lecirelina - Gestran Plus®) na indução da ovulação em fêmeas adultas de avestruz. Primeiramente, um grupo de 10 fêmeas foi avaliado para determinação de seu status reprodutivo, por meio da mensuração das concentrações séricas de estradiol e progesterona (coletas de sangue 3x/semana); da observação do comportamento reprodutivo; da determinação do desenvolvimento ovariano por exame ultrassonográfico (a cada 21 dias); e pela postura de ovos. Após o término desta primeira fase, as fêmeas foram separadas em grupo controle (n = 3) e grupo tratado (n = 6). A cada semana, 2 fêmeas do grupo tratado eram selecionadas para a aplicação intramuscular de 1ml (25μl) de lecirelina, e os seus efeitos avaliados pela análise das concentrações séricas de estradiol, progesterona e corticosterona (0h, 6h, 24h, 48h e 96h pós-aplicação); pela verificação do desenvolvimento ovariano e ocorrência de ovulação através de exames ultrassonográficos (24h, 48h e 96h); pela observação de comportamento reprodutivo (0h, 48h e 96h) e pela postura de ovos. O tratamento foi repetido no mesmo animal a cada 21 dias e em cada tratamento realizado, 2 fêmeas do grupo controle eram submetidas às mesmas análises. Os resultados indicam que a aplicação de lecirelina foi capaz de induzir a ovulação em 77% dos tratamentos realizados, com postura de ovos normais em 50% das induções. Assim, o tratamento proposto foi considerado eficiente na indução da ovulação em fêmeas de avestruz desde que seja aplicado em fêmeas que apresentem ovário bem desenvolvido.

The lack of knowledge about the ostrich reproduction delays the development of the ostrich commercial breeding due to a low reproductive performance. The aim of this study was to evaluate the effect of the application of an analog of GnRH (Lecirelin - Gestran Plus®) for induction of ovulation in adult female ostriches. Initially, a group of 10 adult female ostriches were evaluated in order to determine their reproductive status, by measuring serum levels of estradiol and progesterone (blood sampling - 3 times a week), by the observation of reproductive behavior, by the evaluation of the ovarian development through ultrasound examination (repeated every 21 days) and by the egg laying data. After the end of the first phase, female ostriches were separated into a control group (n = 3) and a treated group (n = 6). Every week, two females from the treated group were selected to receive the treatment: an intramuscular application of 1 ml (25μl) of Lecirelin. The effect of the application was evaluated by the analysis of serum concentrations of estradiol, progesterone and corticosterone (0h, 6h, 24h, 48h and 96h post-application), by the verification of the ovarian development and the occurrence of ovulation through ultrasound examinations (24h, 48h and 96h), by the observation of…

Advisors/Committee Members: Guimarães, Marcelo Alcindo de Barros Vaz.

Subjects/Keywords: Análogo de GNRH; Avestruz; GnRH analog; Hormones; Hormônios; Ostrich; Reprodução; Reproduction; Ultrasound; Ultrassom

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, G. C. d. O. e. (2012). Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;

Chicago Manual of Style (16^th Edition):

Silva, Guilherme Costa de Oliveira e. “Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus).” 2012. Doctoral Dissertation, University of São Paulo. Accessed March 07, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;.

MLA Handbook (7^th Edition):

Silva, Guilherme Costa de Oliveira e. “Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus).” 2012. Web. 07 Mar 2021.

Vancouver:

Silva GCdOe. Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus). [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Mar 07]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;.

Council of Science Editors:

Silva GCdOe. Análise ultrassonográfica, comportamental e produtiva dos efeitos do tratamento com análogo de GnRH em fêmeas de avestruz (Struthio camelus). [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-08102012-161442/ ;

23. Pousias, Serafeim. Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών.

Degree: 2019, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας

URL: http://hdl.handle.net/10442/hedi/46215

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Ganirelix is a GnRH antagonist, which is widely used in Assisted Reproduction Technology (ART), for the prevention of premature LH peaks. Kisspeptin is a neuropeptide,… (more)

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Ganirelix is a GnRH antagonist, which is widely used in Assisted Reproduction Technology (ART), for the prevention of premature LH peaks. Kisspeptin is a neuropeptide, which is secreted from the hypothalamus, and may have a crucial role on the onset of puberty in humans and on ovulation in women. The aim of this study was to examine the effect of Ganirelix on the secretion of LH and on follicle maturation in healthy women. Patients and measurements: Ten healthy female volunteers were studied during two menstrual cycles, i.e. cycle 1 (control, spontaneous cycle) and cycle 2 (Ganirelix). In both cycles, blood samples were taken daily starting on cycle day 2 until ovulation occurred on cycle 1 and transvaginal ultrasound was performed on cycle day 8 and 10 and then on a daily basis until ovulation occurred. During cycle 2, all women were given S.C. injections of the GnRH antagonist Ganirelix at the dose of 0,25 mg/day, starting on cycle day 2 until the day of the endogenous LH surge in cycle 1. In all blood samples, FSH, LH, Estradiol, Progesterone and Kisspeptin levels were measured. Results: During treatment with Ganirelix FSH levels remained stable and similar to those in cycle 1. LH levels were reduced in cycle 2 as compared to cycle 1 on days 3, 4, 7 and 9 (P<0,05), with no significant within-cycle variation. Estradiol levels until day 10 did not differ significantly between the two cycles. After day 10, no follicle maturation and no substantial increase in serum estradiol levels were observed in the antagonist cycle (cycle 2), in contrast to cycle 1. In cycle 1, ovulation was observed in 9 women. In cycle 2, one volunteer ovulated, despite the treatment with Ganirelix, and in 8 women ovulation was postponed for a week. Serum Kisspeptin levels were similar in both cycles, except on days 5 and 6 (P<0,05) in which they were higher in cycle 2. There was no significant within-cycle variation in Kisspeptin levels during the follicular phase. No correlation was found between serum FSH, estradiol and progesterone levels and those of Kisspeptin. However, Kisspeptin levels showed a positive correlation with those of LH on cycle days 2 (r=0,89, Ρ<0,01) and 3 (r=0,79, Ρ<0,05). Conclusions: This study shows for the first time that Ganirelix interferes with the selection of the dominant follicle. Since circulating gonadotrophin levels did not change substantially, it is suggested that Ganirelix affects the process of follicle selection by acting on the ovary. This, however, needs to be further investigated. Regarding the role of Kisspeptin in Ganirelix-suppressed gonadotrophin secretion further studies are required.

Ο Ganirelix είναι ένας GnRH ανταγωνιστής που χρησιμοποιείται για να εμποδίσει το πρώιμο κύμα της LH κατά τη διέγερση των ωοθηκών. Η κισσπεπτίνη είναι ένα νευροπεπτίδιο το οποίο εκκρίνεται από την υποθάλαμο και πιθανότατα διαδραματίζει κύριο ρόλο στον άξονα Υποθάλαμος-Υπόφυση-Γονάδων. Σκοπός της παρούσας διδακτορικής διατριβής ήταν να μελετήσει την επίδραση του Ganirelix στην έκκριση των γοναδοτροφινών και στην…

Subjects/Keywords: GnRH ανταγωνιστής; Ωοθυλακική φάση; Ωοθήκη; Ωοθυλάκιο; Ganirelix; GnRH antagonist; Ovary; Follicle; Follicular phase; Ganirelix

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pousias, S. (2019). Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/46215

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pousias, Serafeim. “Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών.” 2019. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed March 07, 2021. http://hdl.handle.net/10442/hedi/46215.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pousias, Serafeim. “Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών.” 2019. Web. 07 Mar 2021.

Vancouver:

Pousias S. Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2019. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10442/hedi/46215.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pousias S. Επίδραση ενός GnRH ανταγωνιστή στην έκκριση της LH φυσιολογικών γυναικών. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2019. Available from: http://hdl.handle.net/10442/hedi/46215

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

24. Guilherme Silva Moura. Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço.

Degree: 2008, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015

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Este trabalho foi realizado com os seguintes objetivos: a) avaliar o efeito do hormônio liberador de gonadotropina (GnRH), administrado em diferentes momentos do manejo reprodutivo,… (more)

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Este trabalho foi realizado com os seguintes objetivos: a) avaliar o efeito do hormônio liberador de gonadotropina (GnRH), administrado em diferentes momentos do manejo reprodutivo, na taxa de prenhez e concentração sérica de P4 em gado de corte mestiço; e b) avaliar o efeito do hormônio liberador de gonadotropinas (GnRH), administrado 12 dias após IATF, sobre os níveis séricos de progesterona e, principalmente, na taxa de prenhez em vacas de corte mestiças. Para o primeiro objetivo, utilizaram-se 82 fêmeas bovinas mestiças (Bos taurus indicus x Bos taurus taurus), alocadas ao acaso, em três tratamentos: Tcontrole (n = 28): os animais foram observados para detecção de estros e inseminados após 8 a 12 horas; TGnRH0 (n = 27): similar ao Tcontrole, com administração de 25 μg de GnRH (Gestran Plus, Lecirelina) no momento da IA; e TGnRH0-12 (n = 27): similar ao Tcontrole, com administração de 25 μg de GnRH no momento da IA e no dia 12 após a IA. No dia 35, após IA, foi feito o diagnóstico de gestação por exames ultrassonográficos pela via transretal. Foi observado que 57,14% das vacas (16/28) no Tcontrole e no TGnRH0 e TGnRH12, 62,96% (17/27) ficaram gestantes após o primeiro serviço. Não foi observada diferença entre os animais dos tratamentos (P >0,05). Também não se observou diferença entre as concentrações de P4 séricas entre os animais dos tratamentos. Concluiu-se que a administração de análogo de GnRH no momento da IA ou 12 dias após a IA não melhorou o desempenho reprodutivo em vacas mestiças, nem a sintese de P4 pelo corpo lúteo. Para o segundo objetivo, foram utilizadas 59 fêmeas bovinas mestiças (Bos taurus indicus x Bos taurus taurus), alocadas ao acaso, em dois tratamentos: TBE (n = 30): no dia 0, inseriu-se o dispositivo intravaginal de progesterona (Primer) mais 2,0 mg de BE (Estrogin), im; no dia 8, retirou-se o PRIMER e aplicaram-se 300 UI de eCG (Novormon) e 0,15 mg de PGF2α (Prolise), im; no dia 9, aplicou-se 1 mg de BE, im, e realizou-se a IATF 48-56 horas após a retirada do PRIMER; e TBEGnRH12 (n = 29): o protocolo foi similar ao do TBE, porém com administração de 25 μg de GnRH (Gestran Plus, Lecirelina) no dia 12 após a IA. No dia 35, após IA, foi feito o diagnóstico de gestação por exames ultrassonográficos pela via transretal. Neste estudo foi observado que 53,33% das vacas (16/30) no TBE e 37,93% (11/29) no TBEGnRH12 ficaram gestantes após o primeiro serviço. Não foi observada diferença entre os animais dos tratamentos (P >0,05). Também não se observou diferença entre as concentrações de P4 entre os animais dos tratamentos (P >0,05). Concluiu-se que a administração do análogo de GnRH, lecirelina, no dia 12, após a IATF, não afetou as taxas de prenhez e nem a concentração de progesterona dos animais dos tratamentos, nos dias 0, 5, 12 e 20 após a IATF.

The experiment was done with the following objectives: a) to evaluate the effect of GnRH administered at different times of the reproductive management on the pregnancy rate and serum P4 concentration; and b) to evaluate the…

Advisors/Committee Members: Giovanni Ribeiro de Carvalho, José Domingos Guimarães, Eduardo Paulino da Costa, Elenice Andrade Moraes e Amorim, Ciro Alexandre Alves Torres.

Subjects/Keywords: GnRH; PRODUCAO ANIMAL; Bovino de corte; IATF; Progesterona; Beef cattle; GnRH; FTAI; Progesterone

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moura, G. S. (2008). Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moura, Guilherme Silva. “Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço.” 2008. Thesis, Universidade Federal de Viçosa. Accessed March 07, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moura, Guilherme Silva. “Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço.” 2008. Web. 07 Mar 2021.

Vancouver:

Moura GS. Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço. [Internet] [Thesis]. Universidade Federal de Viçosa; 2008. [cited 2021 Mar 07]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moura GS. Uso de análogo de GnRH após inseminação convencional e com protocolo de IATF em gado mestiço. [Thesis]. Universidade Federal de Viçosa; 2008. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2015

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Francou, Bruno. Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2016, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2016SACLS101

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Les hypogonadismes hypogonadotropes congénitaux (CHH) sont des maladies héréditaires caractérisées par un déficit de sécrétion des gonadotrophines par l’hypophyse, à l’origine d’une infertilité ou d’une… (more)

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Les hypogonadismes hypogonadotropes congénitaux (CHH) sont des maladies héréditaires caractérisées par un déficit de sécrétion des gonadotrophines par l’hypophyse, à l’origine d’une infertilité ou d’une absence complète de puberté. On distingue les formes isolées avec olfaction normale (nCHH) et les formes syndromiques associant au déficit gonadotrope d’autres signes, tel qu’un défaut d’olfaction dans le cas du syndrome de Kallmann (SK), la forme plus fréquente de CHH. Les gènes identifiés dans le SK participent au développement embryonnaire et les gènes des nCHH sont impliqués dans la régulation de la sécrétion de la GnRH ou de son action. A ce stade, deux populations de neurones hypothalamiques gonadotropes sont connues, le neurone à GnRH et le neurone KNDy, sécrétant les Kisspeptines et la Neurokinine B. On estimait que l’ensemble des gènes identifiés couvraient moins de 20% des étiologies génétiques. L’objectif de ce doctorat était d’étudier prévalences et mécanismes physiopathologiques des gènes connus et d’identifier de nouvelles étiologies génétiques de CHH. Dans la première partie, nous avons caractérisé la fonctionnalité de tous les variants identifiés sur les gènes KISS1R, TACR3 et TAC3. Cela a permis de préciser les prévalences chez 600 patients, d’identifier un profil neuroendocrinien propre à l’altération de la signalisation Neurokinine B et de démontrer l’implication des Kisspeptines au cours de la vie embryonnaire. Enfin, nous proposons un modèle d’interaction entre le neurone à GnRH et le neurone KNDy. Dans la seconde partie, nous avons identifié deux nouveaux gènes, SEMA3A dans une forme familiale de SK et PNPLA6 dans une forme familiale rare de CHH syndromique. En conclusion, notre connaissance accrue des formes génétiques de CHH, a permis de développer un panel d’exome ciblé dédié au diagnostic par séquençage nouvelle génération permettant l’analyse simultanée de gènes candidats et de gènes connus.

Congenital hypogonadotropic hypogonadism (CHH) is characterized by deficient or absent pubertal development due to deficient or absent secretion of the pituitary gonadotropins. The many known genetic causes are generally classified into distinct nosological groups. One comprises abnormalities that affect the pre-natal development or migration of GnRH neurons, the paradigm of which is Kallmann syndrome. The other encompasses molecular abnormalities that only affect hypothalamic GnRH synthesis, GnRH release or GnRH signaling at pituitary level. At this stage, two populations of hypothalamic neurons implicated in a gonadotrop function are identified, GnRH neurons and KNDy neurons secreting kisspeptins and neurokinin B. All of the identified genes would represent less than 20% of genetic etiologies.The aim of this PhD was to study the prevalence and pathophysiology mechanisms of known genes and to identify new genetic etiologies of CHH.In the first part, we characterized the function of all molecular events identified on KISS1R, TACR3 and TAC3 genes. Prevalences were estimated in 600 patients. A…

Advisors/Committee Members: Guiochon-Mantel, Anne (thesis director).

Subjects/Keywords: Hypogonadisme; Génétique; Caractérisation fonctionnelle; GnRH; Kallmann; Kisspeptine; Hypogonadism; Genetics; Functionnal characterization; GnRH; Kallmann; Kisspeptin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Francou, B. (2016). Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLS101

Chicago Manual of Style (16^th Edition):

Francou, Bruno. “Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 07, 2021. http://www.theses.fr/2016SACLS101.

MLA Handbook (7^th Edition):

Francou, Bruno. “Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms.” 2016. Web. 07 Mar 2021.

Vancouver:

Francou B. Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2016SACLS101.

Council of Science Editors:

Francou B. Contribution à la caractérisation de nouveaux gènes impliqués dans les hypogonadismes hypogonadotropes : caractérisation des mécanismes moléculaires et cellulaires : Contribution to the characterization of new genes involved in the hypogonadotropic hypogonadism : characterization of molecular and cellular mechanisms. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLS101

Universidade de Lisboa

26. Madureira, Márcio André Gonçalves. Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy.

Degree: 2012, Universidade de Lisboa

URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709

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Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2012

Recent advances in cancer treatment field allowed significant increase… (more)

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Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2012

Recent advances in cancer treatment field allowed significant increase in the survival rate of patients. However, the patients have commonly faced long-term adverse effects that severly affected the quality of life, specially concerning their fertility. It is established that radio- and chemotherapy treatments can cause a reduction of the ovarian reserve, resulting in a 40-60% rate of premature ovarian failure (POF) in women exposed to these treatments. In order to decrease POF risks, several fertility preservation options were developed: reduction of the exposure to gonadotoxic agents, gametes or embryos cryopreservation, oocyte donation, ovarian tissue cryopreservation and transplant, or a pharmacological protection of the ovaries during chemotherapy. While the last one appears as a less invasive and promising procedure, the studies and clinical trials continue to show inconsistent results, raising an almost 30-year discussion and controversy. The gonadotropin-releasing hormone (GnRH) is a hypothalamic hormone responsible for the releasing of gonadotropins. Due to its pulsatile fashion to induce FSH secretion, it was proposed that a continuous saturation of the receptor by synthetic GnRH analogues (GnRHa) could decrease the gonadotropins release and, therefore, could maintain the ovarian follicular pool at immature stages. Once it was suggested that initial stage follicles were less affected by alkylating agents, the women capacity to conceive could then be preserved. By a multiple approach study design, including histological, immunohistochemical, in vitro and in vivo assays in a mouse model, our group intended to better understand the potential preventive effect of GnRHa on the ovaries exposed to chemotherapy and to evaluate the efficiency of GnRH agonists (AGOs) and antagonists (ANTs) in this indication. Our results suggest, so far, that AGOs (triptorelin) and ANTs (cetrorelix) are not efficient to prevent the follicular depletion induced by a cyclophosphamide (Cy) treatment. Nevertheless, the fertility follow-up, until now, seems to show that the birth rate is not affected by neither Cy nor combined Cy-GnRHa treatment, suggesting that the mouse experimental model is not yet optimal.

Nos últimos anos, foram feitos enormes avanços no que diz respeito aos tratamentos oncológicos. Estes conseguiram aumentar significativamente as taxas de sobrevivência e o aumento da esperança média de vida dos doentes oncológicos. No entanto, este incremento nem sempre foi acompanhado por uma melhoria na qualidade de vida, nomeadamente na preservação da fertilidade das mulheres sujeitas a tratamentos radio- e quimioterapeuticos. Em média, após este tipo de tratamentos, 40-60% das mulheres são diagnosticadas com falência ovárica precoce (FOP), caracterizada essencialmente por uma diminuição acentuada no número de folículos pertencentes à reserva ovárica de mulheres ainda em idade-reprodutora, derivando…

Advisors/Committee Members: Demeestere, Isabelle, Rodrigues, Gabriela.

Subjects/Keywords: Fertilidade; Quimioterapia; Agonistas da GnRH; Antagonistas da GnRH; Teses de Mestrado - 2012

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Madureira, M. A. G. (2012). Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy. (Thesis). Universidade de Lisboa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Madureira, Márcio André Gonçalves. “Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy.” 2012. Thesis, Universidade de Lisboa. Accessed March 07, 2021. http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Madureira, Márcio André Gonçalves. “Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy.” 2012. Web. 07 Mar 2021.

Vancouver:

Madureira MAG. Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy. [Internet] [Thesis]. Universidade de Lisboa; 2012. [cited 2021 Mar 07]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Madureira MAG. Fertility preservation: potential ovarian protective effect of GnRH analogues during chemotherapy. [Thesis]. Universidade de Lisboa; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/7709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Orlauskaitė, Ieva. GnRH agonisto, metų laiko bei kalės veislės dydžio ir amžiaus įtaka ovuliacijos pasireiškimo laikui.

Degree: Master, Veterinary Medicine, 2014, Lithuanian Academic Libraries Network (LABT)

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_134057-38642 ;

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Šio darbo tikslas buvo nustatyti ovuliacijos pasireiškimo laiko priklausomybę nuo kalės veislės dydžio ir amžiaus bei metų laiko, bei palyginti GnRH agonisto Suprelorin implanto sukeltos… (more)

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Šio darbo tikslas buvo nustatyti ovuliacijos pasireiškimo laiko priklausomybę nuo kalės veislės dydžio ir amžiaus bei metų laiko, bei palyginti GnRH agonisto Suprelorin implanto sukeltos ovuliacijos pasireiškimo laiką su natūraliai pasireiškusios rujos ovuliacijos laiku. Šiam tikslui pasiekti pirmiausiai buvo ištirta 194 kalių (51 skirtingos veislės) progesterono koncentracija kraujo serume. Iš šių kalių buvo atrinktos tos kalės, kurioms pagal progesterono koncentraciją pasireiškė ovuliacija (53 kalės). Nustatyta, kad progesterono koncentracija kraujo serume siekė 20-30 nmol/l. Kalės buvo suskirstytos į grupes: pagal amžių (2 grupės – iki 5 metų (n=41); 5 metų ir vyresnės (n=12) ), pagal veislės dydį (3 grupės – mažos (n=22), vidutinės (n=14) ir didelės (n=17) veislės), pagal sezoną (4 grupės – žiema (n=3), pavasaris (n=16), ruduo (n=11), vasara (n=23) ). Pagal kiekvieną požymį statistiškai buvo įvertinti ovuliacijos pasireiškimo laiko skirtumai tarp grupių, bei įvertinta ovuliacijos priklausomybė nuo kalės amžiaus, dydžio, metų laiko. Statistiškai patikimų skirtumų tarp grupių ir koreliacinių ryšių tarp ovuliacijos pasireiškimo laiko ir kalės amžiaus ir dydžio, bei metų laiko nebuvo nustatyta. Panašių tyrimų iki šiol buvo atlikta labai mažai, todėl informacija yra vertinga. Paskutiniame tyrimųetape4 kalėms buvo panaudoti GnRH agonisto Suprelorin implatai rujai sukelti. Implantas buvo įvedamas po oda, bambos srityje. Po įvedimo buvo imamas kraujas kelis kartus kas 3-5 dienas,... [toliau žr. visą tekstą]

The main goal of this paper was to determine whether or not there is a significant influence of GnRH agonist, seasonality, age and size of the bitch on ovulation time, and to compare ovulation time induced by GnRH agonist Suprelorin implant and the ovulation time that occured naturally. To achieve this goal we tested the blood of 194 bitches (51 breeds) for progesterone levels in blood serum. Out of these 194 bitches we selected the ones (53 bitches) that ovulated. Meaning, the progesterone levels for these 53 bitches varied between 20-30 nmol/l. These 53 bitches were divided into groups: by age (2 groups – younger than 5 years (n=41); 5 years old and older (n=12),, by size (3 groups – small (n=22), medium (n=14), large (n=17), and by season (4 groups – winter (n=3), spring (n=16), summer (n=11), autumn (n=23). All the groups were statistically analyzed to determine whether or not there is a significant difference between the groups, and to determine if there are correlations between the ovulation time and the size and age of the bitch and time of year (season). No significant difference or correlations were determined. We have found a little information about influence of seasonality, and age and size of the bitch on ovulation time, so the information in this paper is valuable. For the last step to achieving the goal of the paper, GnRH agonist Suprelorin implant was used on 4 bitches. The agonist was implanted subcutaneously around the navel area. After the implantation... [to full text]

Advisors/Committee Members: Jokimas, Juozas (Master’s degree committee chair), Autukaitė, Jurgita (Master’s degree session secretary), Žilaitis, Vytuolis (Master’s degree committee member), Želvytė, Rasa (Master’s degree committee member), Šiugždaitė, Jūratė (Master’s degree committee member), Savickis, Saulius (Master’s degree committee member), Rutkauskas, Arūnas (Master’s degree committee member), Laurusevičius, Saulius (Master’s degree committee member), Riškevičienė, Vita (Master’s thesis supervisor).

Subjects/Keywords: Kalė; Lytinis ciklas; Ovuliacija; GnRH agonistas; Bitch; Sexual cycle; Ovulation; GnRH agonist

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Orlauskaitė, Ieva. (2014). GnRH agonisto, metų laiko bei kalės veislės dydžio ir amžiaus įtaka ovuliacijos pasireiškimo laikui. (Masters Thesis). Lithuanian Academic Libraries Network (LABT). Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_134057-38642 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Orlauskaitė, Ieva. “GnRH agonisto, metų laiko bei kalės veislės dydžio ir amžiaus įtaka ovuliacijos pasireiškimo laikui.” 2014. Masters Thesis, Lithuanian Academic Libraries Network (LABT). Accessed March 07, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_134057-38642 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Orlauskaitė, Ieva. “GnRH agonisto, metų laiko bei kalės veislės dydžio ir amžiaus įtaka ovuliacijos pasireiškimo laikui.” 2014. Web. 07 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Orlauskaitė, Ieva. GnRH agonisto, metų laiko bei kalės veislės dydžio ir amžiaus įtaka ovuliacijos pasireiškimo laikui. [Internet] [Masters thesis]. Lithuanian Academic Libraries Network (LABT); 2014. [cited 2021 Mar 07]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_134057-38642 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Orlauskaitė, Ieva. GnRH agonisto, metų laiko bei kalės veislės dydžio ir amžiaus įtaka ovuliacijos pasireiškimo laikui. [Masters Thesis]. Lithuanian Academic Libraries Network (LABT); 2014. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_134057-38642 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Universidad Nacional de La Plata

28. Risso, Analía Lorena. Uso de análogos de GnRH en el control de la reproduccion indeseada en felinos domésticos.

Degree: 2012, Universidad Nacional de La Plata

URL: http://sedici.unlp.edu.ar/handle/10915/18123 ; https://doi.org/10.35537/10915/18123

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Debido a las características de su ciclo estral, los felinos domésticos (Felis catus) son sumamente prolíficos. Bajo el objetivo general de aportar al control de… (more)

Subjects/Keywords: Veterinaria; antagonista de GnRH; agonista de GnRH; contracepción; Gatos; Hormona Liberadora de Gonadotropina

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Risso, A. L. (2012). Uso de análogos de GnRH en el control de la reproduccion indeseada en felinos domésticos. (Thesis). Universidad Nacional de La Plata. Retrieved from http://sedici.unlp.edu.ar/handle/10915/18123 ; https://doi.org/10.35537/10915/18123

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Risso, Analía Lorena. “Uso de análogos de GnRH en el control de la reproduccion indeseada en felinos domésticos.” 2012. Thesis, Universidad Nacional de La Plata. Accessed March 07, 2021. http://sedici.unlp.edu.ar/handle/10915/18123 ; https://doi.org/10.35537/10915/18123.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Risso, Analía Lorena. “Uso de análogos de GnRH en el control de la reproduccion indeseada en felinos domésticos.” 2012. Web. 07 Mar 2021.

Vancouver:

Risso AL. Uso de análogos de GnRH en el control de la reproduccion indeseada en felinos domésticos. [Internet] [Thesis]. Universidad Nacional de La Plata; 2012. [cited 2021 Mar 07]. Available from: http://sedici.unlp.edu.ar/handle/10915/18123 ; https://doi.org/10.35537/10915/18123.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Risso AL. Uso de análogos de GnRH en el control de la reproduccion indeseada en felinos domésticos. [Thesis]. Universidad Nacional de La Plata; 2012. Available from: http://sedici.unlp.edu.ar/handle/10915/18123 ; https://doi.org/10.35537/10915/18123

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Malone, Samuel Andrew. Morphological, molecular and genetic aspects of the GnRH neuronal migratory process in mice and humans : Étude anatomique, moléculaire et génétique de migration des neurones à GnRH chez la souris et l'homme.

Degree: Docteur es, Neurosciences, 2017, Université Lille II – Droit et Santé

URL: http://www.theses.fr/2017LIL2S031

► Chez les mammifères, le contrôle de la reproduction est médié par un réseau hypothalamique qui intègre divers stimuli pour réguler la sécrétion de la Gonadotropin… (more)

▼ Chez les mammifères, le contrôle de la reproduction est médié par un réseau hypothalamique qui intègre divers stimuli pour réguler la sécrétion de la Gonadotropin Releasing Hormone (GnRH). Ces neurones à GnRH naissent dans la placode olfactive et migrent vers le cerveau le long des axones vomeronasaux et terminaux au cours du développement embryonnaire. Bien que ce processus a bien été étudié chez les rongeurs, sa caractérisation complète chez l'homme reste inachevée. Il est largement admis que des perturbations dans le développement ou dans la sécrétion de GnRH sont associées chez l’homme à un hypogonadisme hypogonadotrope congénital (CHH), qui est un trouble caractérisé par un retard ou une absence de la puberté conduisant à l'infertilité.Les systèmes GnRH et olfactif ont des liens complexes au cours du développement, le syndrome de Kallmann (KS) représente un trouble qui associe l'hypogonadisme dû à une déficience en GnRH et l'anosmie. Le CHH et le KS sont des troubles oligogéniques, les mutations génétiques sous-jacentes n’expliquent que 50% des cas cliniques.Dans cette étude, nous avons entrepris une caractérisation complète du processus migratoire des neurones à GnRH au cours du premier trimestre de gestation sur une grande série d'embryons et de fœtus humains, ce qui nous a permis d’élaborer le premier atlas chronologique et quantitatif de la distribution de GnRH. En effet, l'utilisation d’une nouvelle approche de transparisation des tissus embryologiques humains par de solvants organiques, a permis d’établir pour la première fois, une véritable représentation des neurones dans leur contexte natal in vivo.De plus, les résultats de cette étude ont non seulement révélé que le nombre de neurones GnRH chez l'homme était significativement plus élevé que prévu, mais aussi que ces derniers migrent vers plusieurs régions du cerveau extra-hypothalamique, en plus de l'hypothalamus. Leur présence dans ces régions soulève l'hypothèse qu’ils pourraient exercer des rôles non reproductifs, créant de nouvelles pistes pour la recherche sur les fonctions du système GnRH dans les processus cognitifs, comportementaux et physiologiques.Le second objectif de ce travail a visé à caractériser un nouveau gène candidat impliqué dans le développement du système GnRH: L'hormone Anti-Müllerienne (AMH), connue pour son rôle dans la différenciation de la gonade bipotentielle chez les mâles. Néanmoins, une récente étude menée par notre équipe a mis en évidence son rôle extragonadique sur les neurones à GnRH en période post-natale.Le séquençage complet d'une large cohorte de patients européens a révélé plusieurs nouvelles mutations faux-sens dans le gène de l’AMH chez les patients atteints de CHH et KS, non retrouvés dans la cohorte des témoins. L’évaluation de la pertinence fonctionnelle de ces mutations a ensuite été effectuée par diverses analyses biochimiques in vitro de la bioactivité des mutations, ainsi que par la caractérisation d'une lignée de souris transgénique. Ce qui a entraîné une diminution de la sécrétion de l'AMH et une… Advisors/Committee Members: Giacobini, Paolo (thesis director).

Subjects/Keywords: GnRH; CHH; KS; AMH; DISCO; Dveloppement; GnRH; CHH; KS; AMH; DISCO; Development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Malone, S. A. (2017). Morphological, molecular and genetic aspects of the GnRH neuronal migratory process in mice and humans : Étude anatomique, moléculaire et génétique de migration des neurones à GnRH chez la souris et l'homme. (Doctoral Dissertation). Université Lille II – Droit et Santé. Retrieved from http://www.theses.fr/2017LIL2S031

Chicago Manual of Style (16^th Edition):

Malone, Samuel Andrew. “Morphological, molecular and genetic aspects of the GnRH neuronal migratory process in mice and humans : Étude anatomique, moléculaire et génétique de migration des neurones à GnRH chez la souris et l'homme.” 2017. Doctoral Dissertation, Université Lille II – Droit et Santé. Accessed March 07, 2021. http://www.theses.fr/2017LIL2S031.

MLA Handbook (7^th Edition):

Malone, Samuel Andrew. “Morphological, molecular and genetic aspects of the GnRH neuronal migratory process in mice and humans : Étude anatomique, moléculaire et génétique de migration des neurones à GnRH chez la souris et l'homme.” 2017. Web. 07 Mar 2021.

Vancouver:

Malone SA. Morphological, molecular and genetic aspects of the GnRH neuronal migratory process in mice and humans : Étude anatomique, moléculaire et génétique de migration des neurones à GnRH chez la souris et l'homme. [Internet] [Doctoral dissertation]. Université Lille II – Droit et Santé 2017. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2017LIL2S031.

Council of Science Editors:

Malone SA. Morphological, molecular and genetic aspects of the GnRH neuronal migratory process in mice and humans : Étude anatomique, moléculaire et génétique de migration des neurones à GnRH chez la souris et l'homme. [Doctoral Dissertation]. Université Lille II – Droit et Santé 2017. Available from: http://www.theses.fr/2017LIL2S031

30. Calvez, Marie-Laure. Etude de la régulation du canal CFTR impliqué dans la mucoviscidose par un analogue de la GnRHet le Mg2+ : Study of the regulation by a GnRH analog and Mg2+ of the CFTR Cl- channel involved in cystic fibrosis.

Degree: Docteur es, Biologie-santé, 2017, Brest

URL: http://www.theses.fr/2017BRES0048

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La mucoviscidose est la maladie héréditaire autosomique récessive, rare, létale, la plus fréquente dans la population caucasienne. Cette maladie est causée par des mutations du… (more)

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La mucoviscidose est la maladie héréditaire autosomique récessive, rare, létale, la plus fréquente dans la population caucasienne. Cette maladie est causée par des mutations du gène CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) codant la protéine CFTR. Cette protéine est principalement un canal chlorure (Cl-) AMPc-dépendant localisé dans la membrane apicale des cellules épithéliales. La mutation F508del entraîne un défaut de maturation de la protéine qui est retenue dans le réticulum endoplasmique avant d’être dégradée. Cependant, une faible quantité de protéines malformées échappe à ce système de contrôle et parvient à la membrane plasmique.Des travaux de notre équipe ont montré une augmentation des efflux ioniques dépendants du CFTR dans des lignées cellulaires épithéliales bronchiques (CFBE41o-), exprimant le CFTR sauvage ou le CFTR muté F508del, après un traitement par une hormone: la gonadolibérine (GnRH, Gonadotropin releasing hormone, 1h, 10-9M). Cette augmentation est vraisemblablement due à un nombre plus important de canaux CFTR à la membrane plasmique.L’objectif de cette thèse a été de tester un analogue de la GnRH comme modulateur de l’exportation membranaire et/ou de l’activité canal du CFTR muté, sur des cultures primaires de cellules épithéliales nasales humaines homozygotes pour la mutation F508del. Dans un premier temps, nous avons vérifié la présence du récepteur à la GnRH (R-GnRH) dans notre modèle cellulaire. Puis, nous avons étudié l’effet de l’analogue sur la fonction du CFTR par des techniques d’électrophysiologie. Nous avons observé une augmentation des efflux d’ions Cl- médiés par le canal CFTR après un traitement à l’analogue (2h, 10-12M). Enfin, une étude protéomique nous a permis d’identifier des protéines différentiellement exprimées après traitement. Certaines protéines mises en évidence pourraient appartenir à des voies de signalisation intracellulaires ayant un rôle dans la régulation de la protéine CFTR et être des cibles thérapeutiques.Par ailleurs, le canal CFTR est régulé par le Mg2+ intracellulaire ([Mg2+]i). Le canal TRPM7 est le principal régulateur du [Mg2+]i. La [Mg2+]i a été mesurée et l’expression de TRPM7 vérifiée dans des cellules Hela transfectées avec le CFTR sauvage (wt) ou mutés (G551D et F508del). Nous avons étudié la localisation, la fonction et la régulation de TRPM7 dans nos modèles cellulaires avant de rechercher un possible lien fonctionnel entre le CFTR et TRPM7. Dans les modèles CF, l’expression, la fonction et la localisation du canal TRPM7 sont altérées. Il existerait un lien fonctionnel entre TRPM7 et le CFTR par l’intermédiaire de la diminution du [Mg2+]i impliquant TRPM7 dans la physiopathologie de la mucoviscidose.

Cystic fibrosis is the most common lethal autosomal recessive disease in the Caucasian population. This disease is caused by mutations in the gene encoding the CFTR (Cystic Fibrosis Transmembrane conductance Regulator) protein. This protein is a cAMP-regulated chloride channel expressed at the apical membrane of epithelial…

Advisors/Committee Members: Trouvé, Pascal (thesis director).

Subjects/Keywords: CFTR; Mutation F508del; GnRH; Cellules nasales humaines; Analogue; R-GnRH; Mg2+; TRPM7; CFTR; F508del mutation; GnRH; Human nasal epithelial cells; Analogue; GnRHR; Mg2+; TRPM7; 616.372

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Calvez, M. (2017). Etude de la régulation du canal CFTR impliqué dans la mucoviscidose par un analogue de la GnRHet le Mg2+ : Study of the regulation by a GnRH analog and Mg2+ of the CFTR Cl- channel involved in cystic fibrosis. (Doctoral Dissertation). Brest. Retrieved from http://www.theses.fr/2017BRES0048

Chicago Manual of Style (16^th Edition):

Calvez, Marie-Laure. “Etude de la régulation du canal CFTR impliqué dans la mucoviscidose par un analogue de la GnRHet le Mg2+ : Study of the regulation by a GnRH analog and Mg2+ of the CFTR Cl- channel involved in cystic fibrosis.” 2017. Doctoral Dissertation, Brest. Accessed March 07, 2021. http://www.theses.fr/2017BRES0048.

MLA Handbook (7^th Edition):

Calvez, Marie-Laure. “Etude de la régulation du canal CFTR impliqué dans la mucoviscidose par un analogue de la GnRHet le Mg2+ : Study of the regulation by a GnRH analog and Mg2+ of the CFTR Cl- channel involved in cystic fibrosis.” 2017. Web. 07 Mar 2021.

Vancouver:

Calvez M. Etude de la régulation du canal CFTR impliqué dans la mucoviscidose par un analogue de la GnRHet le Mg2+ : Study of the regulation by a GnRH analog and Mg2+ of the CFTR Cl- channel involved in cystic fibrosis. [Internet] [Doctoral dissertation]. Brest; 2017. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2017BRES0048.

Council of Science Editors:

Calvez M. Etude de la régulation du canal CFTR impliqué dans la mucoviscidose par un analogue de la GnRHet le Mg2+ : Study of the regulation by a GnRH analog and Mg2+ of the CFTR Cl- channel involved in cystic fibrosis. [Doctoral Dissertation]. Brest; 2017. Available from: http://www.theses.fr/2017BRES0048

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