You searched for subject:(Genetic regulation).
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1.
Guay, Catherine L., 1979-.
High-throughput tools for functional genomics.
Degree: PhD, Computational and Integrative Biology, 2018, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/57076/ 
► Genetic information is stored in DNA sequences, referred to as the genome. Decoding the meaning of these sequences is a critical challenge in biology. The…
(more)
▼ Genetic information is stored in DNA sequences, referred to as the genome. Decoding the meaning of these sequences is a critical challenge in biology. The genome contains functional components that modulate information in the genome into cell type specific functions. Of these components, Cis-regulatory modules (CRMs) integrate transcription factor inputs to causally affect gene expression. The genomic locations and sequences of CRMs are useful for resolving interactions within gene regulatory networks, and are therefore useful to address fundamental questions pertaining to development, evolution, cancer, and genetic disease. My work addressed major methodological limitations in CRM analysis. Previously, there was no functional method to identify CRMs at genome-scale. Thus, I developed a Genome-scale Reporter Assay Method for CRMs, or GRAMc, that utilizes random 25bp DNA barcodes (N25s) as reporters to quantitatively and reproducibly identify CRMs across an entire genome. The method was applied in cultured human liver cells and in sea urchin embryos. Due to the limited delivery rate of reporter constructs into embryos, it is often advantageous to test GRAMc libraries containing long (>1kb) genomic inserts. Previously, there was no efficient way to characterize genome-scale reporter libraries containing long inserts. To overcome this limitation, I developed a bi-directional mate-pair library approach to characterize long insert containing libraries of genome-scale magnitude. Although genome-scale identification of CRMs is critical to increase cis-regulatory analysis, in embryos it is also necessary to characterize the spatial activity of CRMs. Available methods to identify the spatial activity of CRMs rely on image analysis. Due to the limited number of optically distinct reporter genes, image-based methods are limited to testing only a few CRMs at a time. To address this challenge, I developed a method for Multiplex and Mosaic Observation of Spatial information encoded In CRMs, or MMOSAIC, that increases throughput of spatial cis-regulatory analysis in embryos by several orders of magnitude over traditional imaging based approaches. Together, these tools for cis-regulatory analysis overcome several major limitations for the study of functional genomics
Advisors/Committee Members: Nam, Jongmin (chair).
Subjects/Keywords: Genetic regulation
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APA (6th Edition):
Guay, Catherine L., 1. (2018). High-throughput tools for functional genomics. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/57076/
Chicago Manual of Style (16th Edition):
Guay, Catherine L., 1979-. “High-throughput tools for functional genomics.” 2018. Doctoral Dissertation, Rutgers University. Accessed January 26, 2020.
https://rucore.libraries.rutgers.edu/rutgers-lib/57076/.
MLA Handbook (7th Edition):
Guay, Catherine L., 1979-. “High-throughput tools for functional genomics.” 2018. Web. 26 Jan 2020.
Vancouver:
Guay, Catherine L. 1. High-throughput tools for functional genomics. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2020 Jan 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57076/.
Council of Science Editors:
Guay, Catherine L. 1. High-throughput tools for functional genomics. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57076/
University of Hong Kong
2.
Law, Pui-pik.
Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals.
Degree: PhD, 2016, University of Hong Kong
URL: Law,
P.
[羅珮碧].
(2016).
Investigation
into
the
role
of
heterochromatin
protein
1
gamma
(HP1[gamma])
in
gene
regulation
in
mammals.
(Thesis).
University
of
Hong
Kong,
Pokfulam,
Hong
Kong
SAR.
Retrieved
from
http://dx.doi.org/10.5353/th_b5807288.
;
http://dx.doi.org/10.5353/th_b5807288
;
http://hdl.handle.net/10722/240428
► Heterochromatin protein 1 (HP1) was identified as a key component of the condensed DNA surrounding centromeres in most eukaryotic cells. Genes placed in close proximity…
(more)
▼ Heterochromatin protein 1 (HP1) was identified as a
key component of the condensed DNA surrounding centromeres in most
eukaryotic cells. Genes placed in close proximity to such
constitutively dense heterochromatin regions were found to be
stochastically silenced in a proportion of the cells leading to
variegation of expression, a classical epigenetic phenomenon known
as position effect variegation (PEV). Mutagenesis screens
identified HP1 and SUV39H as powerful suppressors of variegation
which were necessary for heterochromatin-mediated PEV. SUV39H was
found to methylate the histone H3 tail at lysine 9 at
heterochromatin providing a binding site for HP1. This discovery
provided the first direct evidence for a histone or epigenetic code
in which the ‘writer’ of the code on chromatin would be the SUV39H
and the ‘reader’ HP1. This mechanism was subsequently found to be
conserved from S. pombe to humans. A mechanism which shares
features with PEV has also been implicated in the pathogenesis of
repeat-expansion diseases, such as Friedreich’s ataxia, in which
the affected genes are aberrantly silenced. In mice there are 3
highly conserved HP1 isoforms, HP1α, HP1β and HP1γ. HP1α and β are
found by microscopy to be localised to constitutive heterochromatic
condensed regions in the nucleus whereas HP1γ has a pan-nuclear
distribution and is therefore implicating in regulating euchromatic
genes. There is a paucity of data examining how and where HP1γ
regulates gene expression in vivo at the genome-wide level. This
thesis employed knockout and knockdown strategies to investigate
this. In view of the early lethality of mice in which HP1γ has been
deleted by homologous recombination, the function of HP1γ was
studied here by establishing mouse embryonic fibroblast cell lines.
Immunoflourescent microscopy revealed for the first time that HP1γ
was necessary for the localisation of HP1β at constitutive
heterochromatic regions in the nucleus in most cells. This
delocalisation of HP1β was associated with aberrant upregulation of
the repetitive major satellite DNA associated with pericentromeric
heterochromatin implying for the first time that HP1γ plays an
important role in maintaining centromeric heterochromatin in a
silenced state thought to be important for the maintenance of
genome integrity. Strikingly, analysis of the transcriptome
revealed a large number of genes (4293) to be dysregulated in male
cells compared to females (1186) where the effect of HP1γ
deficiency resulted in aberrant expression of immune related genes
that would normally be repressed. This sexually dimorphic effect
was investigated further by studying the effect of HP1γ deficiency
on the subset of 176 genes found to differ in expression between
normal males and females. Moreover, HP1γ in males was found
essential for maintaining the relative repression of 114 genes in
males compared to females, suggesting that the Y chromosome
interacts with HP1γ to reduce the expression of these genes. In
summary, a novel function for HP1γ in repressing pericentromeric
DNA and…
Subjects/Keywords: Genetic regulation; Heterochromatin
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Law, P. (2016). Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals. (Doctoral Dissertation). University of Hong Kong. Retrieved from Law, P. [羅珮碧]. (2016). Investigation into the role of heterochromatin protein 1 gamma (HP1[gamma]) in gene regulation in mammals. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5807288. ; http://dx.doi.org/10.5353/th_b5807288 ; http://hdl.handle.net/10722/240428
Chicago Manual of Style (16th Edition):
Law, Pui-pik. “Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals.” 2016. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
Law, P. [羅珮碧]. (2016). Investigation into the role of heterochromatin protein 1 gamma (HP1[gamma]) in gene regulation in mammals. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5807288. ; http://dx.doi.org/10.5353/th_b5807288 ; http://hdl.handle.net/10722/240428.
MLA Handbook (7th Edition):
Law, Pui-pik. “Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals.” 2016. Web. 26 Jan 2020.
Vancouver:
Law P. Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals. [Internet] [Doctoral dissertation]. University of Hong Kong; 2016. [cited 2020 Jan 26].
Available from: Law, P. [羅珮碧]. (2016). Investigation into the role of heterochromatin protein 1 gamma (HP1[gamma]) in gene regulation in mammals. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5807288. ; http://dx.doi.org/10.5353/th_b5807288 ; http://hdl.handle.net/10722/240428.
Council of Science Editors:
Law P. Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals. [Doctoral Dissertation]. University of Hong Kong; 2016. Available from: Law, P. [羅珮碧]. (2016). Investigation into the role of heterochromatin protein 1 gamma (HP1[gamma]) in gene regulation in mammals. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5807288. ; http://dx.doi.org/10.5353/th_b5807288 ; http://hdl.handle.net/10722/240428
University of Hong Kong
3.
Qin, Jing.
Application of bioinformatics on gene regulation studies
and regulatory network construction with omics data.
Degree: PhD, 2013, University of Hong Kong
URL: Qin,
J.
[覃静].
(2013).
Application
of
bioinformatics
on
gene
regulation
studies
and
regulatory
network
construction
with
omics
data.
(Thesis).
University
of
Hong
Kong,
Pokfulam,
Hong
Kong
SAR.
Retrieved
from
http://dx.doi.org/10.5353/th_b5108668
;
http://dx.doi.org/10.5353/th_b5108668
;
http://hdl.handle.net/10722/205684
► Gene expression is a multi-step process that involves various regulators. From whole genome sequences to the complex gene regulatory system, high-throughput technologies have generated a…
(more)
▼ Gene expression is a multi-step process that
involves various regulators. From whole genome sequences to the
complex gene regulatory system, high-throughput technologies have
generated a large amount of omics data, but information in such a
large scale is hard to interpret manually. Bioinformatics can help
to process this huge biological information and infer biological
insights using the merits of mathematics, statistics and
computational techniques. In this study, we applied various
bioinformatic techniques on gene regulation in several aspects.
Multiple primary transcripts of a gene can be initiated at
different promoters, termed alternative promoters (APs). Most human
genes have multiple APs. However, whether the usage of APs is
independent or not is still controversial. In this study, we
analyze the roles of APs in gene regulations using various
bioinformatics approaches. Chromosomal interactions between APs are
found to be more frequent than interactions between different
genes. By comparing the APs at two ends of the genes, we find that
they are significant different in terms of sequence content,
conservation and motif frequency. The position and distance of two
APs are important for their combined effects, which prove their
regulations are not independent and one AP could affect the
transcription of the other.
With the aim to understand the
multi-level gene regulatory system in various biological processes,
a mass of high-throughput omics data have been generated. However,
each omics technology measuring the molecular abundance or behavior
at a single level has a limited ability to depict the multi-level
system. Integrating omics data can effectively comprehend the
multi-level gene regulatory system and reduce the false positives.
In this study, two web servers, ChIP-Array and ProteoMirExpress,
have been built to construct transcriptional and
post-transcriptional regulatory networks by integrating omics data.
ChIP-Array is a web server for biologists to construct a
TF-centered network for their own data. Network library is further
constructed by ChIP-Array from publicly available data. Given a
series mRNA expression profiles in a biological process, master
regulators can be identified by matching the profiles with the
networks in the library. To explore gene regulatory network
controlled by multiple TFs, least absolute shrinkage and selection
operator (LASSO)-type regularization models are applied on multiple
integrative data. Golden standard based evaluations demonstrate
that the L0 and L1/2 regularization models are efficient and
applicable to gene regulatory network inference in large genome
with a small number of samples. ProteoMirExpress integrates
transcriptomic and proteomic data to infer miRNA-centered networks.
It successfully infers the perturbed miRNA and those that
co-express with it. The resulting network reports miRNA targets
with uncorrelated mRNA and protein levels, which are usually
ignored by tools considering only the mRNA abundance, even though
some of them may be important…
Subjects/Keywords: Bioinformatics; Genetic regulation - Data processing
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Qin, J. (2013). Application of bioinformatics on gene regulation studies
and regulatory network construction with omics data. (Doctoral Dissertation). University of Hong Kong. Retrieved from Qin, J. [覃静]. (2013). Application of bioinformatics on gene regulation studies and regulatory network construction with omics data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108668 ; http://dx.doi.org/10.5353/th_b5108668 ; http://hdl.handle.net/10722/205684
Chicago Manual of Style (16th Edition):
Qin, Jing. “Application of bioinformatics on gene regulation studies
and regulatory network construction with omics data.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
Qin, J. [覃静]. (2013). Application of bioinformatics on gene regulation studies and regulatory network construction with omics data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108668 ; http://dx.doi.org/10.5353/th_b5108668 ; http://hdl.handle.net/10722/205684.
MLA Handbook (7th Edition):
Qin, Jing. “Application of bioinformatics on gene regulation studies
and regulatory network construction with omics data.” 2013. Web. 26 Jan 2020.
Vancouver:
Qin J. Application of bioinformatics on gene regulation studies
and regulatory network construction with omics data. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2020 Jan 26].
Available from: Qin, J. [覃静]. (2013). Application of bioinformatics on gene regulation studies and regulatory network construction with omics data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108668 ; http://dx.doi.org/10.5353/th_b5108668 ; http://hdl.handle.net/10722/205684.
Council of Science Editors:
Qin J. Application of bioinformatics on gene regulation studies
and regulatory network construction with omics data. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Qin, J. [覃静]. (2013). Application of bioinformatics on gene regulation studies and regulatory network construction with omics data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108668 ; http://dx.doi.org/10.5353/th_b5108668 ; http://hdl.handle.net/10722/205684
University of Aberdeen
4.
Khanolkar, Rahul Chaitanya.
Molecular analysis of ABIN1 expression and immunosuppressive function in immature myeloid cells.
Degree: 2013, University of Aberdeen
URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202767
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589494
► The leukocyte immunoglobulin like receptors (LILRs) are a group of receptors with immunomodulatory effects. Group 1 LILRs comprise of LILRB1, among others, and bind to…
(more)
▼ The leukocyte immunoglobulin like receptors (LILRs) are a group of receptors with immunomodulatory effects. Group 1 LILRs comprise of LILRB1, among others, and bind to class 1 MHC molecules and transmits inhibitory signals. Studies have shown that LILRB1 ligation during the monocyte differentiation process into dendritic cells (DCs) results in the generation of a population of cells that are tolerogenic. Here we hypothesize that this tolerogenic nature of the resultant cells is due to the high expression of nuclear factor kappa – light chain enhancer of activated B cells (NF-κB) inhibitor – A20 binding inhibitor of NF-κB signalling 1 (ABIN1). In this study we analyzed the effect that ABIN1 exerts on the maturation of DCs and CD14+HLA-DRlow/- monocytes - a population of cells that have been recently been identified as myeloid derived suppressor cells (MDSCs) in humans. LILRB1 ligated DCs and CD14+HLA-DRlow/- monocytes, when treated with ABIN1 siRNA, displayed an increase in the expression of antigen presentation and co-stimulatory molecules such as CD80, CD86, HLA-DR and HLA-ABC and displayed a greater capacity to produce cytokines like IL-12 and IFN-α. Additionally, they displayed a greater capacity to stimulate the adaptive component of the immune system in terms of IFN-γ production, cell proliferation and adapter molecule and mitogen activated protein kinase (MAPK) activation in T cells. Based on the results we obtained, it can be concluded that ABIN1 plays a significant role in maintaining the immature and suppressive phenotype of immature myeloid cells (IMCs) by dampening NF-κB signalling, while also exerting a negative effect on antiviral signalling.
Subjects/Keywords: Immunosuppression; Dendritic cells; Genetic regulation
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APA (6th Edition):
Khanolkar, R. C. (2013). Molecular analysis of ABIN1 expression and immunosuppressive function in immature myeloid cells. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202767 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589494
Chicago Manual of Style (16th Edition):
Khanolkar, Rahul Chaitanya. “Molecular analysis of ABIN1 expression and immunosuppressive function in immature myeloid cells.” 2013. Doctoral Dissertation, University of Aberdeen. Accessed January 26, 2020.
http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202767 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589494.
MLA Handbook (7th Edition):
Khanolkar, Rahul Chaitanya. “Molecular analysis of ABIN1 expression and immunosuppressive function in immature myeloid cells.” 2013. Web. 26 Jan 2020.
Vancouver:
Khanolkar RC. Molecular analysis of ABIN1 expression and immunosuppressive function in immature myeloid cells. [Internet] [Doctoral dissertation]. University of Aberdeen; 2013. [cited 2020 Jan 26].
Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202767 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589494.
Council of Science Editors:
Khanolkar RC. Molecular analysis of ABIN1 expression and immunosuppressive function in immature myeloid cells. [Doctoral Dissertation]. University of Aberdeen; 2013. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202767 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589494
University of Florida
5.
Melanson, Nicole Marie.
Identification of Chemicals Impairing the Activity of Selected Candidatus Liberibacter asiaticus Transcription Factors.
Degree: 2012, University of Florida
URL: http://ufdc.ufl.edu/AA00061301
► The purpose of this project was to identify chemical ligands that modulate the activity of selected Candidatus Liberibacter asiaticus (CLas) transcriptional regulators. CLas is an…
(more)
▼ The purpose of this project was to identify chemical ligands that modulate the activity of selected Candidatus Liberibacter asiaticus (CLas) transcriptional regulators. CLas is an unculturable organism that causes Huanglongbing disease in citrus. The aim of this project is to identify ligands that may disrupt the activities of transcriptional regulators, and thus may also impair the physiological activities of this bacterium in citrus plants. Due to its reduced genome, CLas has only a few transcriptional regulators, and those selected for this study were CLIBASIA_00835, CLIBASIA_03370, and CLIBASIA_01180. The specific goals were to clone, express and purify CLIBASIA_03370 and CLIBASIA_00835 using Sinorhizobium meliloti as an expression host. The rationale is that by using a host that is phylogenetically related to CLas the solubility of the proteins would be improved, compared to the low expression and solubility previously obtained in Escherchia coli. Preliminary data identified five chemicals that modified the thermostability of purified CLIBASIA_01180. Thus, the second specific goal was to validate the effects of these chemicals in vivo that were found to decrease the DNA-binding activity of CLIBASIA_01180 in vitro. The results obtained indicate that the CLas genes can be expressed in S. meliloti. The CLIBASIA_00835, as well as CLIBASIA_03370, were expressed and partially purified. Additionally, the preliminary screening showed that some of the chemicals affect the in vivo activity of an ortholog of CLIBASIA_01180 in S. meliloti. ( en )
Subjects/Keywords: Genetic transcription – Regulation; Ligands (Biochemistry)
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APA ·
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APA (6th Edition):
Melanson, N. M. (2012). Identification of Chemicals Impairing the Activity of Selected Candidatus Liberibacter asiaticus Transcription Factors. (Thesis). University of Florida. Retrieved from http://ufdc.ufl.edu/AA00061301
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Melanson, Nicole Marie. “Identification of Chemicals Impairing the Activity of Selected Candidatus Liberibacter asiaticus Transcription Factors.” 2012. Thesis, University of Florida. Accessed January 26, 2020.
http://ufdc.ufl.edu/AA00061301.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Melanson, Nicole Marie. “Identification of Chemicals Impairing the Activity of Selected Candidatus Liberibacter asiaticus Transcription Factors.” 2012. Web. 26 Jan 2020.
Vancouver:
Melanson NM. Identification of Chemicals Impairing the Activity of Selected Candidatus Liberibacter asiaticus Transcription Factors. [Internet] [Thesis]. University of Florida; 2012. [cited 2020 Jan 26].
Available from: http://ufdc.ufl.edu/AA00061301.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Melanson NM. Identification of Chemicals Impairing the Activity of Selected Candidatus Liberibacter asiaticus Transcription Factors. [Thesis]. University of Florida; 2012. Available from: http://ufdc.ufl.edu/AA00061301
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Montana State University
6.
Saley, Tara Carolyne.
Introducing the ArsR regulated arsenic stimulon.
Degree: College of Agriculture, 2017, Montana State University
URL: https://scholarworks.montana.edu/xmlui/handle/1/13486
► The United States EPA ranks arsenic as the number one environmental toxin. Since microorganisms are significant drivers of arsenic toxicity and mobility in nature, it…
(more)
▼ The United States EPA ranks arsenic as the number one environmental toxin. Since microorganisms are significant drivers of arsenic toxicity and mobility in nature, it is important to understand how microbes detect and react to arsenic. The microbial arsenic resistance operon (ars) is critical for sensing arsenic in the environment and controlling the cellular response to this toxin. The ars operon is minimally comprised of arsRBC, which codes for an ArsR transcriptional repressor, arsenite effluxer, and an arsenate reductase, respectively, with the operon negatively regulated by the transcriptional repressor, ArsR. Our model organism Agrobacterium tumefaciens 5A carries two ars operons, with each containing two arsR genes. We conducted an RNASeq study to examine the regulatory roles of the encoded four ArsR regulatory proteins as a function of +/- arsenite. We report that the regulatory influence of the ArsR proteins extends well beyond the ars operon, with both activation and repression effects. In addition to the expected arsenic resistance response, many cellular functions were impacted, including: phosphate acquisition/metabolism, sugar transport, chemotaxis, copper tolerance, and iron homeostasis. Each of the ArsR proteins uniquely influenced different sets of genes and an arsR regulatory hierarchy was observed, wherein ArsR1 is auto regulatory and negatively regulates arsR4, ArsR4 activates arsR2, and ArsR2 negatively regulates arsR3. ArsR3 is the least active with respect to number of genes regulated. To summarize, this study provides a more complete understanding of how microbial gene expression and biogeochemical cycling may be influenced by arsenic in the environment.
Advisors/Committee Members: Chairperson, Graduate Committee: Timothy McDermott (advisor).
Subjects/Keywords: Genetic regulation.; Arsenic.; Toxicology.; Bacteria.
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Saley, T. C. (2017). Introducing the ArsR regulated arsenic stimulon. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/13486
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Saley, Tara Carolyne. “Introducing the ArsR regulated arsenic stimulon.” 2017. Thesis, Montana State University. Accessed January 26, 2020.
https://scholarworks.montana.edu/xmlui/handle/1/13486.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Saley, Tara Carolyne. “Introducing the ArsR regulated arsenic stimulon.” 2017. Web. 26 Jan 2020.
Vancouver:
Saley TC. Introducing the ArsR regulated arsenic stimulon. [Internet] [Thesis]. Montana State University; 2017. [cited 2020 Jan 26].
Available from: https://scholarworks.montana.edu/xmlui/handle/1/13486.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Saley TC. Introducing the ArsR regulated arsenic stimulon. [Thesis]. Montana State University; 2017. Available from: https://scholarworks.montana.edu/xmlui/handle/1/13486
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Columbia University
7.
Seeley, John.
microRNA Regulation of Endotoxin Tolerance.
Degree: 2014, Columbia University
URL: https://doi.org/10.7916/D8HX19V9
► Sepsis affects hundreds of thousands each year in the United States alone, with an estimated 20-30% mortality rate in spite of current treatment regimens. Sepsis…
(more)
▼ Sepsis affects hundreds of thousands each year in the United States alone, with an estimated 20-30% mortality rate in spite of current treatment regimens. Sepsis mortality was originally understood to be the caused by overproduction of inflammatory cytokines in response to pathogen detection by the host. However, recent studies suggest that with modern treatments, secondary infection, rather than inflammatory shock, may be of greater concern. In either case, the failure of a large number of anti-inflammatory agents to produce beneficial outcomes in sepsis treatment during clinical trial suggests that the development of a new class of immunomodulatory agents may be required for effective treatment.
In experimental models, pre-treatment with sub-lethal doses of lipopolysaccharide (LPS, previously referred to as endotoxin) induces a state of "LPS tolerance" that reduces septic shock lethality. Paradoxically, LPS tolerance also results in increased antimicrobial gene expression and resistance to secondary infection in some models. Further exploration of this process may provide drug targets capable of limiting inflammation without dampening antimicrobial immunity, which could be of great benefit in the treatment of sepsis and chronic inflammatory disease.
Many groups have studied signaling changes that occur during LPS tolerance. However, mediators of tolerance that can account for the changes in LPS-induced gene expression that result in increased microbial resistance are not well described. This has prevented proper testing of the physiological effects of tolerance on disease, and it remains unclear if this process could be artificially induced or is of any benefit to sepsis patients.
Recent in vitro work suggests that tolerant gene expression patterns are the result of large scale changes in chromatin organization that occur in macrophages after prolonged LPS stimulation. Because microRNAs (miRNAs), a new class of gene regulator, have been found to regulate chromatin modifying complexes in other systems, LPS-induced miRNAs were screened to identify potential mediators of tolerance that could cause changes in gene expression patterns without necessarily impacting LPS signaling itself.
Several tolerance-associated miRNAs were identified. One miRNA in particular, miR-222, was found to repress tumor necrosis factor (Tnf) and Brahma-related gene one (Brg1) expression. This attenuates expression of genes dependent on nucleosome remodeling, primarily affecting inflammatory genes. Consequently, miR-222 expression effectively limits septic shock lethality. However, low-level responses, as well as NF-κB signaling and the expression of a subset of antimicrobial and antiviral genes, are left intact. Thus, although miR-222 does not entirely recapitulate the tolerance response, by directing the LPS response into a less damaging expression profile, miR-222 may accelerate the onset of tolerance and be a promising target for therapeutics aiming to treat inflammatory disease without compromising…
Subjects/Keywords: Endotoxins; Septicemia; Genetic regulation; Immunology
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APA (6th Edition):
Seeley, J. (2014). microRNA Regulation of Endotoxin Tolerance. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8HX19V9
Chicago Manual of Style (16th Edition):
Seeley, John. “microRNA Regulation of Endotoxin Tolerance.” 2014. Doctoral Dissertation, Columbia University. Accessed January 26, 2020.
https://doi.org/10.7916/D8HX19V9.
MLA Handbook (7th Edition):
Seeley, John. “microRNA Regulation of Endotoxin Tolerance.” 2014. Web. 26 Jan 2020.
Vancouver:
Seeley J. microRNA Regulation of Endotoxin Tolerance. [Internet] [Doctoral dissertation]. Columbia University; 2014. [cited 2020 Jan 26].
Available from: https://doi.org/10.7916/D8HX19V9.
Council of Science Editors:
Seeley J. microRNA Regulation of Endotoxin Tolerance. [Doctoral Dissertation]. Columbia University; 2014. Available from: https://doi.org/10.7916/D8HX19V9
Columbia University
8.
Lee, Andreia H.
Regulation of Proviral Expression and Post-Translational Modifications in Embryonic Cells.
Degree: 2017, Columbia University
URL: https://doi.org/10.7916/D8S46ZCB
► Moloney Murine Leukemia Virus (M-MLV) proviral DNA is transcriptionally silenced in mouse embryonic cells by a repressor complex containing tripartite-motif-containing 28 (Trim28). Trim28 depends on…
(more)
▼ Moloney Murine Leukemia Virus (M-MLV) proviral DNA is transcriptionally silenced in mouse embryonic cells by a repressor complex containing tripartite-motif-containing 28 (Trim28). Trim28 depends on post-translational modifications, such as sumoylation and phosphorylation, and its interactions with several co-repressor proteins to regulate its repressive activity. YY1 is one such Trim28 co-repressor protein, recently found to tether the Trim28 silencing complex to the M-MLV promoter. Here, we investigated the biochemical interaction of Trim28 and YY1, and the role of sumoylation and phosphorylation of Trim28 in mediating M-MLV silencing. Experiments probing the binding of YY1 and Trim28 in vitro suggested that their interaction occurs indirectly. Mutational studies demonstrated that the RBCC domain of Trim28 is sufficient for interaction with YY1 while the acidic region 1 and zinc fingers of YY1 were necessary and sufficient for its interaction with Trim28. Additionally, we found that the K779 residue was critical for Trim28-mediated silencing of M-MLV in embryonic cells.
The repressor complex that silences M-MLV is very large and likely consists of many protein subunits. A few proteins contained in the repressor complex have been identified, including Trim28, but the identity of most of the components forming the repressor complex are unknown. We detected a new form of the complex that is of even high molecular weight and likely contains additional associated cofactors. We reported an approach for purifying this larger repressor complex and identified new candidates for cofactors that may potentially function in the silencing of M-MLV.
We also examined the regulation of sumoylation in embryonic cells. Sumoylation conjugation is a post-translational modification that affects a diverse range of processes and is important for embryo survival. Overall inhibition of the SUMO pathways results in embryonic lethality demonstrating the importance of the SUMO pathways for embryonic viability; however, our understanding of SUMO function in embryos at the cellular and molecular level is still greatly lacking. We demonstrated that SUMO1 cannot be overexpressed in embryonic carcinoma and embryonic stem cells and that SUMO1 overexpression is prevented at a post-transcriptional level. This occurred specifically for SUMO1 and not for SUMO2 overexpression. Furthermore, blocking conjugation or increasing the deconjugation of SUMO1 to substrates significantly improved SUMO1 overexpression. The results indicate that the overexpression of SUMO1 protein, in itself, is tolerated in embryonic cells, but the accumulation of substrate(s) modified by SUMO1 appears to be strongly prevented by an embryonic-specific post-transcriptional mechanism.
Subjects/Keywords: Biology; Virology; Cytology; Genetic regulation
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APA (6th Edition):
Lee, A. H. (2017). Regulation of Proviral Expression and Post-Translational Modifications in Embryonic Cells. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8S46ZCB
Chicago Manual of Style (16th Edition):
Lee, Andreia H. “Regulation of Proviral Expression and Post-Translational Modifications in Embryonic Cells.” 2017. Doctoral Dissertation, Columbia University. Accessed January 26, 2020.
https://doi.org/10.7916/D8S46ZCB.
MLA Handbook (7th Edition):
Lee, Andreia H. “Regulation of Proviral Expression and Post-Translational Modifications in Embryonic Cells.” 2017. Web. 26 Jan 2020.
Vancouver:
Lee AH. Regulation of Proviral Expression and Post-Translational Modifications in Embryonic Cells. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2020 Jan 26].
Available from: https://doi.org/10.7916/D8S46ZCB.
Council of Science Editors:
Lee AH. Regulation of Proviral Expression and Post-Translational Modifications in Embryonic Cells. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D8S46ZCB
East Carolina University
9.
Vick, Stephen.
VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN
MANIPULATION IN DEVELOPING SYNOVIAL JOINTS.
Degree: 2012, East Carolina University
URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14830
► Little is understood about the complex process of synovial joint formation in early limb development. It has been shown that versican is highly expressed in…
(more)
▼ Little is understood about the complex process of
synovial joint formation in early limb development. It has been
shown that versican is highly expressed in the extracellular matrix
of these joints (Snow et al. 2005; Shepard et al. 2007) and the
knockdown of versican leads to malformation of the interzone
tissues that form the articular cartilage and synovial cavity
(Nagchowdhuri et al. 2012). It is also thought that these effects
impact gene expression in cells that are involved in the developing
joint. Versican is chondroitin sulfate proteoglycan. It has four
functional-modules: the N-terminal domain the C-terminal domain and
GAG-[alpha] and GAG-[beta] chondroitin sulfate attachment regions.
The N-terminal domain is also known as the G1 domain and the
C-terminal domain is also known as the G3 domain (Kimata et al.
1986 and Zimmerman et al. 1989). In previous studies versican
protein expression has been reduced and the G1 domain has been
over-expressed to observe how these changes in the developing joint
tissue effect gene expression. This study is a validation of
microarray data specifically focusing on hyaluronan and Wnt pathway
genes as they pertain to the misexpression of versican. RT-PCR and
real-time PCR were used in this study to validate expression of the
genes chosen. Through the use of these techniques the degree of
expression has been quantified and compared to the fold changes
observed with the microarray data. Overall G1 versican mediated
gene expression in the developing joint with regard to hyaluronan
and Wnt pathway transcript
regulation were in agreement with
results obtained by RNA array.; Biology, Molecular biology,
Developmental biology, Hyaluronan, Joint Development,
Versican
Advisors/Committee Members: Anthony A. Capehart (advisor).
Subjects/Keywords: Genetic regulation; Joints; Chondroitin sulfates
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APA (6th Edition):
Vick, S. (2012). VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN
MANIPULATION IN DEVELOPING SYNOVIAL JOINTS. (Masters Thesis). East Carolina University. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14830
Chicago Manual of Style (16th Edition):
Vick, Stephen. “VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN
MANIPULATION IN DEVELOPING SYNOVIAL JOINTS.” 2012. Masters Thesis, East Carolina University. Accessed January 26, 2020.
http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14830.
MLA Handbook (7th Edition):
Vick, Stephen. “VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN
MANIPULATION IN DEVELOPING SYNOVIAL JOINTS.” 2012. Web. 26 Jan 2020.
Vancouver:
Vick S. VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN
MANIPULATION IN DEVELOPING SYNOVIAL JOINTS. [Internet] [Masters thesis]. East Carolina University; 2012. [cited 2020 Jan 26].
Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14830.
Council of Science Editors:
Vick S. VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN
MANIPULATION IN DEVELOPING SYNOVIAL JOINTS. [Masters Thesis]. East Carolina University; 2012. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=14830
University of Aberdeen
10.
Hay, Elizabeth A.
The effects of genetic and epigenetic variation on the control of the cannabinoid-1 receptor gene and their role in disease and drug efficacy.
Degree: PhD, 2019, University of Aberdeen
URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=241466
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774032
► Gene regulatory DNA regions are essential for controlling gene expression and they contain a significant proportion of disease-associated variants. Understanding variation within gene regulatory regions…
(more)
▼ Gene regulatory DNA regions are essential for controlling gene expression and they contain a significant proportion of disease-associated variants. Understanding variation within gene regulatory regions may facilitate understanding of disease progression or the development of stratified medicines. The CB1 receptor is involved in controlling many physiological processes such as appetite and reward signalling and has been investigated as a drug target. Differences in response and side effects, however, may indicate that genetic variation within the CB1 receptor gene (CNR1), may need to be investigated to better predict disease and drug efficacy. As disease-associated polymorphisms have been identified in non-coding sequences of CNR1, the aim was to investigate the role of CNR1 regulatory elements and how genetic and epigenetic variation influence their activity. This study identified that the CNR1 promoter is active in primary cell cultures generated from the hypothalamus and dorsal root ganglia (DRGs) of rats and is highly susceptible to CpG methylation in DRGs. Additionally, a highly conserved intronic sequence, ECR1, was disrupted in mice using the CRISPR/Cas9 system. Disruption of ECR1 led to reduced CNR1 expression in the hippocampus but not in the hypothalamus as well as reduced CB1 agonistinduced hypothermia, a reduction in ethanol intake and altered anxiety-like behaviour. The major allele of a SNP within ECR1, permitted CNR1 promoter activity and the minor allele significantly repressed CNR1 promoter activity in hypothalamic and DRG cell cultures. In DRGs, allelic variation within ECR1 altered the response of the CNR1 promoter to the CB1 receptor antagonist/inverse agonist, rimonabant. In conclusion, this study indicates that ECR1 is a tissue specific enhancer and may be involved in regulating physiological processes, and, genetic variation in ECR1 can alter its control of the CNR1 promoter. Overall, this study elucidates possible CNR1 gene regulatory mechanisms, and how genetic and epigenetic variation influences gene regulation.
Subjects/Keywords: Genetic regulation; Cannabinoids; Drug receptors
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hay, E. A. (2019). The effects of genetic and epigenetic variation on the control of the cannabinoid-1 receptor gene and their role in disease and drug efficacy. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=241466 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774032
Chicago Manual of Style (16th Edition):
Hay, Elizabeth A. “The effects of genetic and epigenetic variation on the control of the cannabinoid-1 receptor gene and their role in disease and drug efficacy.” 2019. Doctoral Dissertation, University of Aberdeen. Accessed January 26, 2020.
http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=241466 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774032.
MLA Handbook (7th Edition):
Hay, Elizabeth A. “The effects of genetic and epigenetic variation on the control of the cannabinoid-1 receptor gene and their role in disease and drug efficacy.” 2019. Web. 26 Jan 2020.
Vancouver:
Hay EA. The effects of genetic and epigenetic variation on the control of the cannabinoid-1 receptor gene and their role in disease and drug efficacy. [Internet] [Doctoral dissertation]. University of Aberdeen; 2019. [cited 2020 Jan 26].
Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=241466 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774032.
Council of Science Editors:
Hay EA. The effects of genetic and epigenetic variation on the control of the cannabinoid-1 receptor gene and their role in disease and drug efficacy. [Doctoral Dissertation]. University of Aberdeen; 2019. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=241466 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774032
11.
NC DOCKS at East Carolina University; Vick, Stephen.
VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS.
Degree: 2012, NC Docks
URL: http://libres.uncg.edu/ir/ecu/f/0000-embargo-holder.txt
► Little is understood about the complex process of synovial joint formation in early limb development. It has been shown that versican is highly expressed in…
(more)
▼ Little is understood about the complex process of synovial joint formation in early limb development. It has been shown that versican is highly expressed in the extracellular matrix of these joints (Snow et al. 2005; Shepard et al. 2007) and the knockdown of versican leads to malformation of the interzone tissues that form the articular cartilage and synovial cavity (Nagchowdhuri et al. 2012). It is also thought that these effects impact gene expression in cells that are involved in the developing joint. Versican is chondroitin sulfate proteoglycan. It has four functional-modules: the N-terminal domain the C-terminal domain and GAG-[alpha] and GAG-[beta] chondroitin sulfate attachment regions. The N-terminal domain is also known as the G1 domain and the C-terminal domain is also known as the G3 domain (Kimata et al. 1986 and Zimmerman et al. 1989). In previous studies versican protein expression has been reduced and the G1 domain has been over-expressed to observe how these changes in the developing joint tissue effect gene expression. This study is a validation of microarray data specifically focusing on hyaluronan and Wnt pathway genes as they pertain to the misexpression of versican. RT-PCR and real-time PCR were used in this study to validate expression of the genes chosen. Through the use of these techniques the degree of expression has been quantified and compared to the fold changes observed with the microarray data. Overall G1 versican mediated gene expression in the developing joint with regard to hyaluronan and Wnt pathway transcript regulation were in agreement with results obtained by RNA array.
Subjects/Keywords: Genetic regulation; Joints; Chondroitin sulfates
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❌
APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
NC DOCKS at East Carolina University; Vick, S. (2012). VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/ecu/f/0000-embargo-holder.txt
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
NC DOCKS at East Carolina University; Vick, Stephen. “VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS.” 2012. Thesis, NC Docks. Accessed January 26, 2020.
http://libres.uncg.edu/ir/ecu/f/0000-embargo-holder.txt.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
NC DOCKS at East Carolina University; Vick, Stephen. “VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS.” 2012. Web. 26 Jan 2020.
Vancouver:
NC DOCKS at East Carolina University; Vick S. VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS. [Internet] [Thesis]. NC Docks; 2012. [cited 2020 Jan 26].
Available from: http://libres.uncg.edu/ir/ecu/f/0000-embargo-holder.txt.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
NC DOCKS at East Carolina University; Vick S. VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS. [Thesis]. NC Docks; 2012. Available from: http://libres.uncg.edu/ir/ecu/f/0000-embargo-holder.txt
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rutgers University
12.
Caratozzolo, Rose Marie, 1978-.
Studies of polyadenylation regulation of U1A mRNA by an RNP complex containing U1A and U1 snRNP.
Degree: PhD, Biochemistry, 2011, Rutgers University
URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057530
► The 3’-end processing of nearly all eukaryotic pre-mRNAs comprises multiple steps which culminate in the addition of a poly(A) tail, which is essential for mRNA…
(more)
▼ The 3’-end processing of nearly all eukaryotic pre-mRNAs comprises multiple steps which culminate in the addition of a poly(A) tail, which is essential for mRNA stability, translation, and export. Consequently, polyadenylation regulation is an important component of gene expression. One way to regulate polyadenylation is to inhibit the activity of a single poly(A) site, as exemplified by the U1A protein that negatively autoregulates itself by binding to a Polyadenylation Inhibitory Element (PIE) site within the 3’ UTR of its own pre-mRNA. U1 snRNP, which is primarily involved in splice site recognition, inhibits poly(A) site activity in papillomaviruses by binding to 5’ splice site-like sequences, which have recently been named “U1-sites”. Here, a recently identified U1-site in the human U1A 3'UTR is examined and shown to synergize with the adjacent PIE site to inhibit polyadenylation. However, unlike the sites found in papillomaviruses, the U1A U1-site has no inhibitory activity on its own and is dependent on a wild-type PIE. This lack of activity is due to the site being masked within a phylogenetically conserved stem structure (U1-STEM). The secondary RNA structure of this region was confirmed by RNase digestion analysis. Mutation of the U1-STEM, thereby opening up the U1-site, greatly increases U1-site mediated inhibition. The region between the U1-STEM and PIE (referred to as Region C) was also revealed to be required for synergy. Since biotin pulldown assays indicated that U1 snRNP binding to the U1-site was not affected by the presence of the U1-STEM, a model was proposed suggesting that U1 snRNP binds to the U1-STEM, but remains trapped in an inactive conformation until PIE is bound by two U1A molecules. However, further experiments showed that U1 snRNP binding did actually increase when the U1-STEM was mutated, but no corresponding change to the U1-STEM structure was detected. The discrepancies within these data suggest there is still much to be determined regarding the binding of U1 snRNP to the U1-Site. A more refined model is then presented which involves remodeling of Region C and part of the U1-STEM.
Advisors/Committee Members: Caratozzolo, Rose Marie, 1978- (author), GUNDERSON, SAMUEL I (chair), Brewer, Gary (internal member), Covey, Lori (internal member), Tian, Bin (outside member).
Subjects/Keywords: Gene expression; Genetic regulation; RNA
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APA ·
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MLA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Caratozzolo, Rose Marie, 1. (2011). Studies of polyadenylation regulation of U1A mRNA by an RNP complex containing U1A and U1 snRNP. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057530
Chicago Manual of Style (16th Edition):
Caratozzolo, Rose Marie, 1978-. “Studies of polyadenylation regulation of U1A mRNA by an RNP complex containing U1A and U1 snRNP.” 2011. Doctoral Dissertation, Rutgers University. Accessed January 26, 2020.
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057530.
MLA Handbook (7th Edition):
Caratozzolo, Rose Marie, 1978-. “Studies of polyadenylation regulation of U1A mRNA by an RNP complex containing U1A and U1 snRNP.” 2011. Web. 26 Jan 2020.
Vancouver:
Caratozzolo, Rose Marie 1. Studies of polyadenylation regulation of U1A mRNA by an RNP complex containing U1A and U1 snRNP. [Internet] [Doctoral dissertation]. Rutgers University; 2011. [cited 2020 Jan 26].
Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057530.
Council of Science Editors:
Caratozzolo, Rose Marie 1. Studies of polyadenylation regulation of U1A mRNA by an RNP complex containing U1A and U1 snRNP. [Doctoral Dissertation]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057530
Rutgers University
13.
Li, Ying, 1987-.
Gene regulation during central nervous system development and post-injury regeneration.
Degree: PhD, Biomedical Engineering, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49255/
► Central nervous system (CNS) development and post-injury neurogenesis require accurate coordination of neural stem cell proliferation, progenitor cell differentiation, neuron, glia migration and maturation, and…
(more)
▼ Central nervous system (CNS) development and post-injury neurogenesis require accurate coordination of neural stem cell proliferation, progenitor cell differentiation, neuron, glia migration and maturation, and synapse formation between axons and dendrites. Such systems with high complexity require strict temporal and spatial control via several levels of regulation, in which the transcription regulation is one of the most critical steps. The developmental and injury-repair process involves over 18,000 genes, for majority of which the molecular mechanism governing their transcription remains largely unknown. In an attempt to address this question, four projects were conducted focusing on two levels of transcription regulation: i.e., chromatin modification, and the interaction of cis-acting regulatory sequences with trans-acting protein factors. Computational methods were adopted to analyze the sequences of the cis-elements and iii make predictions for their interacting transcription factors (TFs). The functional roles of these cis- and trans-elements were further determined in vivo and in vitro. The following findings are presented: 1) the function of DNA topoisomerase II beta (Top2b) in proper laminar formation and cell survival during retinal development; 2) the development of computational method for identifying gene regulatory networks involving enhancers and master TFs that are important in retinal cell differentiation; 3) the mechanism of Notch1 regulation in neural stem/progenitor cells via the interaction between Nkx6.1 and a CNS specific enhancer CR2 during the development of the spinal cord interneurons; and 4) the role of CR2 in aNSC activation after injury. Findings from this dissertation provide new insights into the molecular mechanisms underlying transcription regulation during CNS development and post-injury neurogenesis. They can also serve as a basis for future development of gene therapies and regenerative medicine for neurological disorders including spinal cord injury.
Advisors/Committee Members: Cai, Li (chair), Firestein, Bonnie (internal member), Grumet, Martin (internal member), Kwan, Kelvin (outside member), Rasin, Mladen-Roko (outside member).
Subjects/Keywords: Genetic regulation; Developmental neurobiology
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, Ying, 1. (2016). Gene regulation during central nervous system development and post-injury regeneration. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49255/
Chicago Manual of Style (16th Edition):
Li, Ying, 1987-. “Gene regulation during central nervous system development and post-injury regeneration.” 2016. Doctoral Dissertation, Rutgers University. Accessed January 26, 2020.
https://rucore.libraries.rutgers.edu/rutgers-lib/49255/.
MLA Handbook (7th Edition):
Li, Ying, 1987-. “Gene regulation during central nervous system development and post-injury regeneration.” 2016. Web. 26 Jan 2020.
Vancouver:
Li, Ying 1. Gene regulation during central nervous system development and post-injury regeneration. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2020 Jan 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49255/.
Council of Science Editors:
Li, Ying 1. Gene regulation during central nervous system development and post-injury regeneration. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49255/
Rutgers University
14.
Dirschbacher, Nina, 1991-.
Topology of contacts in interacting polymers.
Degree: MS, Physics and Astronomy, 2014, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45235/
► Recent developments in the study of chromatin loop domains and gene regulation motivates us to investigate the topology of contacts in interacting polymers. To describe…
(more)
▼ Recent developments in the study of chromatin loop domains and gene
regulation motivates us to investigate the topology of contacts in interacting polymers. To describe the chromatin loop formation we will use a bead on a string model with excluded volume and attractive polymer-polymer interaction simulated by self avoiding walks. The resulting chain configuration is a balance between these two interactions and can be a coiled state for good solvents or a collapsed one for poor solvent. We can classify the different chain configurations by their topological structures through the genus, a topological invariant of the resulting diagrams. The backtracking algorithm enables us to achieve exact numerical results for the genus distribution of the above mentioned model for small polymer lengths. Since pseudoknots decrease the entropy by restricting the possible configuration, we explore whether we see a suppression of pseudoknots for finite size polymers and intermediate strength of interaction.
Advisors/Committee Members: Sengupta, Anirvan Mayukh (chair), Chandra, Premala (internal member), Alexandre, Morozov V. (internal member).
Subjects/Keywords: Genetic regulation; Chromatin – genetics
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Dirschbacher, Nina, 1. (2014). Topology of contacts in interacting polymers. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45235/
Chicago Manual of Style (16th Edition):
Dirschbacher, Nina, 1991-. “Topology of contacts in interacting polymers.” 2014. Masters Thesis, Rutgers University. Accessed January 26, 2020.
https://rucore.libraries.rutgers.edu/rutgers-lib/45235/.
MLA Handbook (7th Edition):
Dirschbacher, Nina, 1991-. “Topology of contacts in interacting polymers.” 2014. Web. 26 Jan 2020.
Vancouver:
Dirschbacher, Nina 1. Topology of contacts in interacting polymers. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2020 Jan 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45235/.
Council of Science Editors:
Dirschbacher, Nina 1. Topology of contacts in interacting polymers. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45235/
Hong Kong University of Science and Technology
15.
Min, Lan.
The functional roles of α2-chimaerin in the development of hippocampus.
Degree: 2013, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-70593
;
https://doi.org/10.14711/thesis-b1240164
;
http://repository.ust.hk/ir/bitstream/1783.1-70593/1/th_redirect.html
► Adult neurogenesis, generation of mammalian new neurons throughout adulthood, occurs in two restricted regions in the brain- the subventricular zone of lateral ventricle and the…
(more)
▼ Adult neurogenesis, generation of mammalian new neurons throughout adulthood, occurs in two restricted regions in the brain- the subventricular zone of lateral ventricle and the subgranular zone of dentate gyrus in hippocampus. Hippocampal neurogenesis has been suggested to be a new mechanism that mediates learning and memory. It is a complex process including proliferation of neural progenitor cells, neuronal differentiation, maturation and functional integration. To ensure proper neurogenesis, the organization and dynamics of cytoskeletal network has to be precisely regulated and tightly coordinated. A GTPase activating protein (GAP) α2-chimaerin regulates the organization and dynamics of actin and microtubule cytoskeletal network. α2-chimaerin regulates axon guidance in developing corticospinal tract through its GAP activity towards Rac1, and modulates neuronal migration in cerebral cortex via the SH2 domain. While α2-chimaerin is highly expressed in the adult mouse hippocampus, its specific function in hippocampus remains unclear. We aim to investigate whether α2-chimaerin is involved in adult neurogenesis in hippocampus. We found that the number of both newly-born cells and actively proliferating neuronal precursors was dramatically reduced in the subgranular zone of dentate gyrus in adult α2-chimaerin null mice. Furthermore, the number of immature neurons was also reduced. Together, we demonstrated that α2-chimaerin is critical for both amplification of neuronal progenitor cells and neuronal differentiation in adult neurogenesis. In future, we will investigate the mechanisms underlying the α2-chimaerin-mediated regulation of proliferation/differentiation of neuronal precursors and whether the loss of α2-chimearin leads to aberrant brain function in adult mice.
Subjects/Keywords: Sea horses
; Development
; Genetic regulation
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APA (6th Edition):
Min, L. (2013). The functional roles of α2-chimaerin in the development of hippocampus. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-70593 ; https://doi.org/10.14711/thesis-b1240164 ; http://repository.ust.hk/ir/bitstream/1783.1-70593/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Min, Lan. “The functional roles of α2-chimaerin in the development of hippocampus.” 2013. Thesis, Hong Kong University of Science and Technology. Accessed January 26, 2020.
http://repository.ust.hk/ir/Record/1783.1-70593 ; https://doi.org/10.14711/thesis-b1240164 ; http://repository.ust.hk/ir/bitstream/1783.1-70593/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Min, Lan. “The functional roles of α2-chimaerin in the development of hippocampus.” 2013. Web. 26 Jan 2020.
Vancouver:
Min L. The functional roles of α2-chimaerin in the development of hippocampus. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2013. [cited 2020 Jan 26].
Available from: http://repository.ust.hk/ir/Record/1783.1-70593 ; https://doi.org/10.14711/thesis-b1240164 ; http://repository.ust.hk/ir/bitstream/1783.1-70593/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Min L. The functional roles of α2-chimaerin in the development of hippocampus. [Thesis]. Hong Kong University of Science and Technology; 2013. Available from: http://repository.ust.hk/ir/Record/1783.1-70593 ; https://doi.org/10.14711/thesis-b1240164 ; http://repository.ust.hk/ir/bitstream/1783.1-70593/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Hong Kong University of Science and Technology
16.
Lau, Kei Man.
DNA methylation regulates the transcriptional activity of acetylcholinesterase gene during C2C12 myogenesis.
Degree: 2015, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-74370
;
https://doi.org/10.14711/thesis-b1448956
;
http://repository.ust.hk/ir/bitstream/1783.1-74370/1/th_redirect.html
► In vertebrates, acetylcholinesterase (AChE) is well-known for its role in hydrolyzing neurotransmitter acetylcholine at the neuromuscular junction (nmj), which is critical for proper function of…
(more)
▼ In vertebrates, acetylcholinesterase (AChE) is well-known for its role in hydrolyzing neurotransmitter acetylcholine at the neuromuscular junction (nmj), which is critical for proper function of our motor system. Previous studies evidenced that the expression of AChE was developmentally regulated during myogenesis, indicating the significance of intrinsic factors in regulating gene expression. However, these factors involved in the AChE regulation remained unclear. During myogenesis, DNA methylation is essential for temporal control of myogenic gene expression. Vertebrate AChE gene carries highly conserved CG-rich regions in its promoters, implying its likeliness to be methylated. Here, we aimed to investigate the regulation of AChE during myogenesis by DNA methylation. Myogenic differentiation of mouse C2C12 myoblast cells was triggered by serum reduction; the mRNA level, promoter activity, enzymatic activity and protein expression of AChE were up-regulated along with the myotube formation. To investigate the role of DNA methylation in AChE regulation, a DNA methyltransferase inhibitor, 5-Azacytidine (5-Aza), was applied throughout myogenesis. When DNA methylation was inhibited, the promoter activity, transcript expression and enzymatic activity of AChE were markedly increased after day 3 of differentiation, which indicated the putative role of DNA methylation. The effect of 5-Aza led to further investigation on the potential methylated sites on AChE promoter during myogenesis. By bisulfite pyrosequencing, the overall methylation rate was found to peak at day 3 during C2C12 differentiation; a SP1 site on AChE promoter was revealed to be heavily methylated. Additionally, the SP1-driven transcriptional activity was increased in 5-Aza-treated C2C12 culture. By gel mobility shift assay, the DNA methylation on the SP1 site, derived from AChE promoter, totally blocked the binding of SP1. The findings suggest the role of DNA methylation on AChE transcriptional regulation and provide insight for elucidating the DNA methylation-mediated regulatory mechanism on AChE expression during myogenic differentiation.
Subjects/Keywords: Acetylcholinesterase
; Regulation
; Genetic transcription
; Myogenesis
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APA (6th Edition):
Lau, K. M. (2015). DNA methylation regulates the transcriptional activity of acetylcholinesterase gene during C2C12 myogenesis. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-74370 ; https://doi.org/10.14711/thesis-b1448956 ; http://repository.ust.hk/ir/bitstream/1783.1-74370/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lau, Kei Man. “DNA methylation regulates the transcriptional activity of acetylcholinesterase gene during C2C12 myogenesis.” 2015. Thesis, Hong Kong University of Science and Technology. Accessed January 26, 2020.
http://repository.ust.hk/ir/Record/1783.1-74370 ; https://doi.org/10.14711/thesis-b1448956 ; http://repository.ust.hk/ir/bitstream/1783.1-74370/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lau, Kei Man. “DNA methylation regulates the transcriptional activity of acetylcholinesterase gene during C2C12 myogenesis.” 2015. Web. 26 Jan 2020.
Vancouver:
Lau KM. DNA methylation regulates the transcriptional activity of acetylcholinesterase gene during C2C12 myogenesis. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2015. [cited 2020 Jan 26].
Available from: http://repository.ust.hk/ir/Record/1783.1-74370 ; https://doi.org/10.14711/thesis-b1448956 ; http://repository.ust.hk/ir/bitstream/1783.1-74370/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lau KM. DNA methylation regulates the transcriptional activity of acetylcholinesterase gene during C2C12 myogenesis. [Thesis]. Hong Kong University of Science and Technology; 2015. Available from: http://repository.ust.hk/ir/Record/1783.1-74370 ; https://doi.org/10.14711/thesis-b1448956 ; http://repository.ust.hk/ir/bitstream/1783.1-74370/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Columbia University
17.
Rajbhandari, Presha.
Systematic elucidation of transcriptional network necessary for initiation and maintenance of high-risk neuroblastoma.
Degree: 2016, Columbia University
URL: https://doi.org/10.7916/D8J67H0X
► Neuroblastoma is a heterogeneous pediatric malignancy originating from the developing sympathetic nervous system, with poor long-term survival for high-risk patients (~40%). About half of advanced…
(more)
▼ Neuroblastoma is a heterogeneous pediatric malignancy originating from the developing sympathetic nervous system, with poor long-term survival for high-risk patients (~40%). About half of advanced neuroblastomas harbor high-level amplification of the MYCN gene, and these tumors show few, if any, additional driver lesions. Despite significant increase in the body of knowledge of genetics in neuroblastoma, all the high-risk patients follow similar therapeutic procedures and little advancement has been made on molecular target based therapies. The major challenge is to dissect the complexity and heterogeneity of these tumors to find driver genes and activated pathways that are essential for the survival of these cancer cells.
We used an integrated systems biology approach to define the core regulatory machinery responsible for maintenance of an aggressive neuroblastoma phenotypic state. In the first part of the thesis, I will discuss our computational approach to decipher the tumor heterogeneity by subtype classification, followed by identification of master regulator protein modules for three distinct molecular subtypes of high-risk neuroblastomas, which were validated in a large independent cohort of cases. We propose that such modules are responsible for integrating the effect of mutations in upstream pathways and for regulating the genetic programs and pathways necessary for tumor state implementation and maintenance.
The second part of the thesis is focused on experimental validation of putative master regulators in the subtype of neuroblastomas associated with MYCN amplification. By using RNAi screening followed by experimental and computational analyses to elucidate the interdependencies between the top master regulators, we identified TEAD4-MYCN positive feedback loop as a major tumor maintenance mechanism in this subtype. While MYCN regulates TEAD4 transcriptionally, TEAD4 regulates MYCN through transcriptional and post-translational mechanisms. Jointly, MYCN and TEAD4 regulate 90% of inferred MR proteins and causally orchestrate 70% of the subtype-specific gene expression signature. TEAD4 gene expression was associated with neuroblastoma patient survival independently of age, tumor stage and MYCN status (P=2.1e-02). In cellular assays, MYCN promoted growth and repressed differentiation, while TEAD4 activated proliferation and DNA damage repair programs, the signature hallmarks of MYCN-amplified neuroblastoma cells. Specifically, TEAD4 was shown to induce MYCN-independent proliferation by transactivating key genes implicated in high-risk neuroblastoma pathogenesis, including cyclin-dependent kinases, cyclins, E2Fs, DNA replication factors, checkpoint kinases and ubiquitin ligases. The critical role of the core master regulator module in controlling tumor cell viability, both in vitro and in vivo, and its clinical relevance as a prognostic factor highlights TEAD4 as a novel and highly effective candidate target for therapeutic intervention.
In this thesis, we demonstrate that interrogation of…
Subjects/Keywords: Genetic regulation; Carcinogenesis; Neuroblastoma – Genetic aspects; Oncology
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APA ·
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APA (6th Edition):
Rajbhandari, P. (2016). Systematic elucidation of transcriptional network necessary for initiation and maintenance of high-risk neuroblastoma. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8J67H0X
Chicago Manual of Style (16th Edition):
Rajbhandari, Presha. “Systematic elucidation of transcriptional network necessary for initiation and maintenance of high-risk neuroblastoma.” 2016. Doctoral Dissertation, Columbia University. Accessed January 26, 2020.
https://doi.org/10.7916/D8J67H0X.
MLA Handbook (7th Edition):
Rajbhandari, Presha. “Systematic elucidation of transcriptional network necessary for initiation and maintenance of high-risk neuroblastoma.” 2016. Web. 26 Jan 2020.
Vancouver:
Rajbhandari P. Systematic elucidation of transcriptional network necessary for initiation and maintenance of high-risk neuroblastoma. [Internet] [Doctoral dissertation]. Columbia University; 2016. [cited 2020 Jan 26].
Available from: https://doi.org/10.7916/D8J67H0X.
Council of Science Editors:
Rajbhandari P. Systematic elucidation of transcriptional network necessary for initiation and maintenance of high-risk neuroblastoma. [Doctoral Dissertation]. Columbia University; 2016. Available from: https://doi.org/10.7916/D8J67H0X
University of Aberdeen
18.
McFarland, Matthew R.
Transfer RNAs as regulatory agents in the translational control of gene expression.
Degree: PhD, 2016, University of Aberdeen
URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231855
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715449
► Translational efficiency is dictated in part by the availability of charged transfer RNA. Depletion of aminoacylated tRNAs (e.g. during recombinant protein expression) can increase translational…
(more)
▼ Translational efficiency is dictated in part by the availability of charged transfer RNA. Depletion of aminoacylated tRNAs (e.g. during recombinant protein expression) can increase translational errors and associated stress responses. Here, the role of tRNAs as regulators of gene expression was explored through development of synthetic, tRNA-regulated gene circuits, and through an investigation of the impact of tRNA aminoacylation on endogenous gene expression. Synthetic gene circuits initially explored the use of dominant negative alleles of the release factor eRF1 to modulate stop codon readthrough and translationally regulate gene expression. Mutant eRF1 proteins exhibited only a six-fold stimulatory effect on stop codon readthrough. The dominant negative phenotype was rescued partially by overexpression of eRF1, but not eRF3. Ultimately the severity of growth inhibition by these eRF1 alleles limited their utility in synthetic gene circuit design. A novel synthetic circuit was then implemented that utilised TetR interaction with a TetR-inducing peptide in order to control the expression of a suppressor tRNA, and thus a luciferase reporter gene. Using a parameterised mathematical model, the promoter configuration of the circuit was successfully optimised, allowing suppressor tRNAs to regulate the production of luciferase in both feedforward and positive feedback modes of operation. The effects of charged tRNA levels on the global translation network were dissected by regulating the S.cerevisiae glutamine tRNA synthetase gene GLN4 using a tet-off doxycyclineregulated promoter. tRNA synthetase depletion caused the activation of the Gcn4 amino acid starvation response due to accumulation of uncharged glutamine tRNAs. Doxycycline GLN4 shut-off caused increased amino acid production, and decreased ribosome biosynthesis at the transcriptomic and proteomic level, and further physiological changes proposed to result from compromised translation of glutamine-rich regulatory proteins. tRNA overexpression in the GLN4 depletion strain successfully caused altered competition between different isoacceptor tRNA types for their cognate synthetase resource. Together, these results support a growing understanding of tRNA as a key modulator of translation and gene expression in synthetic and natural systems.
Subjects/Keywords: 572.8; Genetic translation; Genetic regulation; Transfer RNA
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APA (6th Edition):
McFarland, M. R. (2016). Transfer RNAs as regulatory agents in the translational control of gene expression. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231855 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715449
Chicago Manual of Style (16th Edition):
McFarland, Matthew R. “Transfer RNAs as regulatory agents in the translational control of gene expression.” 2016. Doctoral Dissertation, University of Aberdeen. Accessed January 26, 2020.
http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231855 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715449.
MLA Handbook (7th Edition):
McFarland, Matthew R. “Transfer RNAs as regulatory agents in the translational control of gene expression.” 2016. Web. 26 Jan 2020.
Vancouver:
McFarland MR. Transfer RNAs as regulatory agents in the translational control of gene expression. [Internet] [Doctoral dissertation]. University of Aberdeen; 2016. [cited 2020 Jan 26].
Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231855 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715449.
Council of Science Editors:
McFarland MR. Transfer RNAs as regulatory agents in the translational control of gene expression. [Doctoral Dissertation]. University of Aberdeen; 2016. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231855 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715449
Washington University in St. Louis
19.
Hoynes-O'Connor, Allison.
Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits.
Degree: PhD, Energy, Environmental & Chemical Engineering, 2016, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/eng_etds/202
► Genetic circuits enable engineers to program complex logical behaviors into living organisms. Organisms can be programmed to optimize the production of fuels and chemicals,…
(more)
▼ Genetic circuits enable engineers to program complex logical behaviors into living organisms. Organisms can be programmed to optimize the production of fuels and chemicals, diagnose and treat diseases, or remediate environmental pollutants. A well-characterized toolbox of
genetic sensors and regulators is needed to construct these circuits.
Genetic sensors that respond to environmentally-relevant signals allow circuits to evaluate the cell's conditions, and versatile and designable regulators translate information about the cell's environment into the desired response. In this work, we demonstrate the de novo design of RNA thermosensors in Escherichia coli, and integrate these sensors into complex
genetic circuits. Next, we provide a large-scale analysis of antisense RNA regulators, generate design rules for these regulators, and validate these design rules through the construction of
genetic circuits with predictable behaviors. Finally AND and NAND gates are developed that respond to temperature and pH, and utilize protein and RNA regulators. The sensors, regulators, and circuits developed and characterized here represent a substantial contribution to the synthetic biology toolbox. Furthermore, this work constitutes an important step forward in enabling
genetic circuits to overcome challenges in chemical synthesis, medicine, and environmental remediation.
Advisors/Committee Members: Tae Seok Moon, Ursula Goodenough, Himadri Pakrasi, Hani Zaher, Fuzhong Zhang.
Subjects/Keywords: Genetic circuit; Genetic engineering; Genetic regulator; Genetic sensor; RNA regulation; Synthetic biology; Engineering
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APA (6th Edition):
Hoynes-O'Connor, A. (2016). Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/202
Chicago Manual of Style (16th Edition):
Hoynes-O'Connor, Allison. “Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits.” 2016. Doctoral Dissertation, Washington University in St. Louis. Accessed January 26, 2020.
https://openscholarship.wustl.edu/eng_etds/202.
MLA Handbook (7th Edition):
Hoynes-O'Connor, Allison. “Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits.” 2016. Web. 26 Jan 2020.
Vancouver:
Hoynes-O'Connor A. Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2016. [cited 2020 Jan 26].
Available from: https://openscholarship.wustl.edu/eng_etds/202.
Council of Science Editors:
Hoynes-O'Connor A. Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits. [Doctoral Dissertation]. Washington University in St. Louis; 2016. Available from: https://openscholarship.wustl.edu/eng_etds/202
University of Utah
20.
Updike, Dustin L.
An analysis of PHA-4 and its cofactors during pharynx development in C; elegans;.
Degree: PhD, Oncological Sciences;, 2006, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/436/rec/25
► As development progresses, cells become specialized and adopt a regional, cellular, tissue, or organ specific fate. This fate is directed by a specialized group of…
(more)
▼ As development progresses, cells become specialized and adopt a regional, cellular, tissue, or organ specific fate. This fate is directed by a specialized group of transcription factors called selector genes. Selector genes act at the top of a hierarchy and orchestrate expression of multiple target genes, thereby enacting developmental differentiation programs. One of these selector genes is the winged helix transcription factor FoxA. FoxA is essential for digestive tract development in all animals that it has been studied. For example, in the nematode Caenorhabditis elegans the FoxA homolog, pha-4, specifies pharyngeal, or foregut, cells. When it is disrupted, cells that would have become the pharynx develop as ectoderm. Despite the importance of FoxA proteins during foregut development, little is known about FoxA regulators and cofactors. To identify cofactors of FoxA, we performed a mutagenesis in C. elegans to screen for loci that interact genetically with pha-4. From this screen, 2 recessive and 11 dominant regulators of PHA-4 expression or activity were identified that suppress lethality associated with a partial loss of pha-4 function. Single nucleotide polymorphisms were used to map the 13 pha-4 suppressors, which showed that at least 9 genes were obtained from the screen. One of the dominant suppressors was identified as an allele of pha-4 itself, which demonstrated the effectiveness of the screen. Next, we looked in depth at a second pha-4 suppressor that was identified as a loss-of-function in the predicted DNA helicase ruvb-1. ruvb-1 is a known component of the Esa1/TIP60 and Swr1/p400 chromatin modifying and remodeling complex in eukaryotes. We investigated the role of chromatin remodeling during pharyngeal development and discovered that homologous components of the Esa1/TIP60 and Swr1/p400 remodeling complex act synergistically with pha-4 by associating the H2A histone variant, HTZ-1/H2AZ, to pharyngeal promoters. These findings are the first to demonstrate an in vivo requirement for chromatin remodeling during foregut development. Finally, we investigated additional phenotypes associated with the ruvb-1 mutant, which resemble mutations in the TOR kinase nutrient sensing pathway in C. elegans. The roles of ruvb-1 and pha-4 in nutrient sensing and metabolism during postembryonic development were examined.
Subjects/Keywords: Genetics; Genetic Regulation
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APA ·
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APA (6th Edition):
Updike, D. L. (2006). An analysis of PHA-4 and its cofactors during pharynx development in C; elegans;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/436/rec/25
Chicago Manual of Style (16th Edition):
Updike, Dustin L. “An analysis of PHA-4 and its cofactors during pharynx development in C; elegans;.” 2006. Doctoral Dissertation, University of Utah. Accessed January 26, 2020.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/436/rec/25.
MLA Handbook (7th Edition):
Updike, Dustin L. “An analysis of PHA-4 and its cofactors during pharynx development in C; elegans;.” 2006. Web. 26 Jan 2020.
Vancouver:
Updike DL. An analysis of PHA-4 and its cofactors during pharynx development in C; elegans;. [Internet] [Doctoral dissertation]. University of Utah; 2006. [cited 2020 Jan 26].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/436/rec/25.
Council of Science Editors:
Updike DL. An analysis of PHA-4 and its cofactors during pharynx development in C; elegans;. [Doctoral Dissertation]. University of Utah; 2006. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/436/rec/25
University of Alberta
21.
Macaulay, Colin G.
Regulation of gene expression in Shope fibroma virus.
Degree: PhD, Department of Biochemistry, 1990, University of Alberta
URL: https://era.library.ualberta.ca/files/p2676x290
Subjects/Keywords: Genetic regulation.; Viruses.
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APA (6th Edition):
Macaulay, C. G. (1990). Regulation of gene expression in Shope fibroma virus. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/p2676x290
Chicago Manual of Style (16th Edition):
Macaulay, Colin G. “Regulation of gene expression in Shope fibroma virus.” 1990. Doctoral Dissertation, University of Alberta. Accessed January 26, 2020.
https://era.library.ualberta.ca/files/p2676x290.
MLA Handbook (7th Edition):
Macaulay, Colin G. “Regulation of gene expression in Shope fibroma virus.” 1990. Web. 26 Jan 2020.
Vancouver:
Macaulay CG. Regulation of gene expression in Shope fibroma virus. [Internet] [Doctoral dissertation]. University of Alberta; 1990. [cited 2020 Jan 26].
Available from: https://era.library.ualberta.ca/files/p2676x290.
Council of Science Editors:
Macaulay CG. Regulation of gene expression in Shope fibroma virus. [Doctoral Dissertation]. University of Alberta; 1990. Available from: https://era.library.ualberta.ca/files/p2676x290
University of Aberdeen
22.
Johansson, Petronella.
Characterisation of putative dendritic cell markers in salmonids and modulation of gene expression following stimulation with interleukin-4/13.
Degree: PhD, 2014, University of Aberdeen
URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=216323
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.633290
► Dendritic cells (DCs) are leukocytes specialized in antigen presentation. As competent stimulators of naive T lymphocytes, they link the innate and adaptive immune responses of…
(more)
▼ Dendritic cells (DCs) are leukocytes specialized in antigen presentation. As competent stimulators of naive T lymphocytes, they link the innate and adaptive immune responses of vertebrates. The RAG-mediated adaptive immune system appeared approximately 500 million years ago in jawed fish and a number of studies suggest that DCs exist in bony and cartilaginous fish. However, the exact role of DCs in the fish immune system is not determined and questions remain as to whether a cell type truly homologous to DCs in homeotherms does exist. My project aimed to identify potential DCs surface markers (CD209A and LAMP3) in rainbow trout (Oncorhynchus mykiss) leukocytes for evaluation of the expression patterns by qRT-PCR under different conditions and stimuli, in vitro and in vivo. Another goal was to validate and evaluate the specificity of a produced anti-trout CD209A polyclonal antibody to further characterise antigen presenting cells (APCs) in fish. The methodology was to look for up-regulation of the predicted markers together with other markers known to be expressed by DCs in mammals and to evaluate at the mRNA and protein expression level after in vitro stimulation of trout primary leukocytes with trout rIL-4/13. Trout CD209A and LAMP3 mRNA was expressed in the main lymphoid organs of fish and could be modulated with microbial mimics. Upon in vitro stimulation of trout primary leukocytes with trout rIL-4/13, trout CD209A mRNA expression was up-regulated together with both CD83 and the MHC class II chain known to be expressed by mammalian DCs. In addition, CD209A protein expression was highly induced by trout rIL-4/13. Taken together, these results suggests that the characterisation of DCs in trout with tools such as transcript evaluation of surface markers and the anti-trout CD209A antibody, could help to more precisely define these leukocyte subsets. These findings could have further impact on fish vaccine improvements and be of importance for the aquaculture industry, by optimising stimulation of adaptive immunity.
Subjects/Keywords: 590; Dendritic cells; Salmonidae; Genetic regulation
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MLA ·
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APA (6th Edition):
Johansson, P. (2014). Characterisation of putative dendritic cell markers in salmonids and modulation of gene expression following stimulation with interleukin-4/13. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=216323 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.633290
Chicago Manual of Style (16th Edition):
Johansson, Petronella. “Characterisation of putative dendritic cell markers in salmonids and modulation of gene expression following stimulation with interleukin-4/13.” 2014. Doctoral Dissertation, University of Aberdeen. Accessed January 26, 2020.
http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=216323 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.633290.
MLA Handbook (7th Edition):
Johansson, Petronella. “Characterisation of putative dendritic cell markers in salmonids and modulation of gene expression following stimulation with interleukin-4/13.” 2014. Web. 26 Jan 2020.
Vancouver:
Johansson P. Characterisation of putative dendritic cell markers in salmonids and modulation of gene expression following stimulation with interleukin-4/13. [Internet] [Doctoral dissertation]. University of Aberdeen; 2014. [cited 2020 Jan 26].
Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=216323 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.633290.
Council of Science Editors:
Johansson P. Characterisation of putative dendritic cell markers in salmonids and modulation of gene expression following stimulation with interleukin-4/13. [Doctoral Dissertation]. University of Aberdeen; 2014. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=216323 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.633290
University of Hong Kong
23.
Deng, Liyu.
Exploration of the transcription factors that regulate
the expression of the haloacid operon in Burkholderia caribensis
MBA4.
Degree: PhD, 2014, University of Hong Kong
URL: Deng,
L.
[鄧麗瑜].
(2014).
Exploration
of
the
transcription
factors
that
regulate
the
expression
of
the
haloacid
operon
in
Burkholderia
caribensis
MBA4.
(Thesis).
University
of
Hong
Kong,
Pokfulam,
Hong
Kong
SAR.
Retrieved
from
http://dx.doi.org/10.5353/th_b5387968
;
http://dx.doi.org/10.5353/th_b5387968
;
http://hdl.handle.net/10722/208618
► Bacterial dehalogenase is a key enzyme involved in bioremediation of halogenated organic compounds. A dehalogenase, Deh4a, was isolated from the Gram-negative bacterium Burkholderia caribensis MBA4,…
(more)
▼ Bacterial dehalogenase is a key enzyme involved in
bioremediation of halogenated organic compounds. A dehalogenase,
Deh4a, was isolated from the Gram-negative bacterium Burkholderia
caribensis MBA4, which can utilize haloacetic acids as carbon
source. The haloacid operon in MBA4 was identified and
characterized. It is composed of the structural genes forDeh4a and
a transporter Deh4p. Transcription of this operon is negatively
regulated, but the mechanism and the relevant regulator are still
poorly understood. In this study, magnetic DNA affinity
chromatography and Tn5transposon mutagenesis were employed to
explore the regulatory factors that affected the expression of this
haloacid operon.
A process that uses lysates from glycolate-grown
cells, magnetic DNA affinity chromatography and LC-MS/MS has
identified a TetR family transcriptional regulator, TetR8620, which
binds to the promoter region of deh4a. Disruption of the TetR8620
gene in mutant Ins8620 abolished the formation of a slow migrating
complex in electrophoretic mobility shift assay (EMSA) using
lysates from glycolate-grown cells. Moreover, expressions of deh4a
were enhanced in bothglycolate- and MCA- grown Ins8620. The
addition of recombinant histidine-tagged TetR8620 to lysates of
Ins8620 resumed the formation of a retardation complex, but
different from that using purified His-tagged TetR8620.This
suggested that TetR8620 is responsible for formation of retardation
complexes, and an additional protein might be involved. To
investigate other putative factors that interact with TetR8620,
purified His-tagged TetR8620 was immobilized with Ni-NTA agarose
and used for isolation of interacting proteins. Chemical
cross-linking of the purified fraction with BS3established that
TetR8620 interacts with a proteinof30 kDa. Separation of the
cross-linked complex in SDS-PAGE gel also showed that a protein
with similar MW was specifically pulled down. These results suggest
that TetR8620 was interacting with a ~30 kDa protein. Protein
identification using mass spectrometry assay proposed that this
protein is probably a universal stress protein UspA encoding by
peg.3485 or acetyl-glutamate kinase (EC 2.7.2.8) encoding by
peg.714 in MBA4.
Tn5transposon mutagenesis was also employed to
explore the factors that regulate the haloacid operon ofMBA4. A
derivative of MBA4, MK06, which contains a kanamycin resistant gene
(kan) with a deh4apromoter was constructed. Kanamycin resistancy of
this derivative was MCA inducible. Transposon mutagenesis was
conducted on this derivative, and Tn-containing mutants were
isolated as tetracycline resistant colonies on pyruvate plates.
These colonies were further selected on their resistance
tokanamycin in pyruvate plates. Gene peg.6589 encoding a putative
transcriptional regulator, DehR1, was disrupted by Tn insertion.
While the production of dehalogenase was still MCA-inducible, this
mutant has partially relieved the repression of the haloacid operon
in media containing pyruvate. Moreover, constitutive production of
DehR1 in MBA4 decreased…
Subjects/Keywords: Transcription factors; Genetic regulation; Bacterial genetics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Deng, L. (2014). Exploration of the transcription factors that regulate
the expression of the haloacid operon in Burkholderia caribensis
MBA4. (Doctoral Dissertation). University of Hong Kong. Retrieved from Deng, L. [鄧麗瑜]. (2014). Exploration of the transcription factors that regulate the expression of the haloacid operon in Burkholderia caribensis MBA4. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5387968 ; http://dx.doi.org/10.5353/th_b5387968 ; http://hdl.handle.net/10722/208618
Chicago Manual of Style (16th Edition):
Deng, Liyu. “Exploration of the transcription factors that regulate
the expression of the haloacid operon in Burkholderia caribensis
MBA4.” 2014. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
Deng, L. [鄧麗瑜]. (2014). Exploration of the transcription factors that regulate the expression of the haloacid operon in Burkholderia caribensis MBA4. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5387968 ; http://dx.doi.org/10.5353/th_b5387968 ; http://hdl.handle.net/10722/208618.
MLA Handbook (7th Edition):
Deng, Liyu. “Exploration of the transcription factors that regulate
the expression of the haloacid operon in Burkholderia caribensis
MBA4.” 2014. Web. 26 Jan 2020.
Vancouver:
Deng L. Exploration of the transcription factors that regulate
the expression of the haloacid operon in Burkholderia caribensis
MBA4. [Internet] [Doctoral dissertation]. University of Hong Kong; 2014. [cited 2020 Jan 26].
Available from: Deng, L. [鄧麗瑜]. (2014). Exploration of the transcription factors that regulate the expression of the haloacid operon in Burkholderia caribensis MBA4. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5387968 ; http://dx.doi.org/10.5353/th_b5387968 ; http://hdl.handle.net/10722/208618.
Council of Science Editors:
Deng L. Exploration of the transcription factors that regulate
the expression of the haloacid operon in Burkholderia caribensis
MBA4. [Doctoral Dissertation]. University of Hong Kong; 2014. Available from: Deng, L. [鄧麗瑜]. (2014). Exploration of the transcription factors that regulate the expression of the haloacid operon in Burkholderia caribensis MBA4. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5387968 ; http://dx.doi.org/10.5353/th_b5387968 ; http://hdl.handle.net/10722/208618
University of Hong Kong
24.
Yuan, Yuan.
Transcriptional regulation of mouse secretin receptor in
hypothalamic cells.
Degree: PhD, 2011, University of Hong Kong
URL: Yuan,
Y.
[袁媛].
(2011).
Transcriptional
regulation
of
mouse
secretin
receptor
in
hypothalamic
cells.
(Thesis).
University
of
Hong
Kong,
Pokfulam,
Hong
Kong
SAR.
Retrieved
from
http://dx.doi.org/10.5353/th_b4775293
;
http://dx.doi.org/10.5353/th_b4775293
;
http://hdl.handle.net/10722/174473
► As a neuropeptide, both secretin and secretin receptor are expressed in the central nervous system (CNS). It has been revealed that the activities of…
(more)
▼ As a neuropeptide, both secretin and secretin
receptor are expressed in the central nervous system (CNS). It has
been revealed that the activities of secretin on hypothalamic cells
of rodents are important for osmoregulation and food intake. In the
present study, embryonic mouse hypothalamic cell line N42 was used
to study the promoter activity of mouse secretin receptor (mSR). By
5′ deletion analysis, a promoter element was identified within ?282
to ?443, relative to the ATG codon, and it contains a GC-box (-297
to -286), a ras responsive element (RRE) (-289 to -276) and an
E-box (-416 to -411). Electrophoretic mobility shift assay (EMSA)
and supershift analyses showed that Sp1 interacted with the GC-box,
another zinc finger As a neuropeptide, both secretin and secretin
receptor are expressed in the central nervous system (CNS). It has
been revealed that the activities of secretin on hypothalamic cells
of rodents are important for osmoregulation and food intake. In the
present study, embryonic mouse hypothalamic cell line N42 was used
to study the promoter activity of mouse secretin receptor (mSR). By
5′ deletion analysis, a promoter element was identified within ?282
to ?443, relative to the ATG codon, and it contains a GC-box (-297
to -286), a ras responsive element (RRE) (-289 to -276) and an
E-box (-416 to -411). Electrophoretic mobility shift assay (EMSA)
and supershift analyses showed that Sp1 interacted with the GC-box,
another zinc finger
published_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy
Advisors/Committee Members: Chow, BKC.
Subjects/Keywords: Genetic transcription - Regulation.; Secretin - Receptors.; Transcription factors.
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yuan, Y. (2011). Transcriptional regulation of mouse secretin receptor in
hypothalamic cells. (Doctoral Dissertation). University of Hong Kong. Retrieved from Yuan, Y. [袁媛]. (2011). Transcriptional regulation of mouse secretin receptor in hypothalamic cells. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775293 ; http://dx.doi.org/10.5353/th_b4775293 ; http://hdl.handle.net/10722/174473
Chicago Manual of Style (16th Edition):
Yuan, Yuan. “Transcriptional regulation of mouse secretin receptor in
hypothalamic cells.” 2011. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
Yuan, Y. [袁媛]. (2011). Transcriptional regulation of mouse secretin receptor in hypothalamic cells. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775293 ; http://dx.doi.org/10.5353/th_b4775293 ; http://hdl.handle.net/10722/174473.
MLA Handbook (7th Edition):
Yuan, Yuan. “Transcriptional regulation of mouse secretin receptor in
hypothalamic cells.” 2011. Web. 26 Jan 2020.
Vancouver:
Yuan Y. Transcriptional regulation of mouse secretin receptor in
hypothalamic cells. [Internet] [Doctoral dissertation]. University of Hong Kong; 2011. [cited 2020 Jan 26].
Available from: Yuan, Y. [袁媛]. (2011). Transcriptional regulation of mouse secretin receptor in hypothalamic cells. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775293 ; http://dx.doi.org/10.5353/th_b4775293 ; http://hdl.handle.net/10722/174473.
Council of Science Editors:
Yuan Y. Transcriptional regulation of mouse secretin receptor in
hypothalamic cells. [Doctoral Dissertation]. University of Hong Kong; 2011. Available from: Yuan, Y. [袁媛]. (2011). Transcriptional regulation of mouse secretin receptor in hypothalamic cells. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775293 ; http://dx.doi.org/10.5353/th_b4775293 ; http://hdl.handle.net/10722/174473
University of Hong Kong
25.
Law, Pui-pik.
Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals.
Degree: PhD, 2016, University of Hong Kong
URL: http://hdl.handle.net/10722/236574
abstract
Paediatrics and Adolescent
Medicine
Doctoral
Doctor of Philosophy
Subjects/Keywords: Genetic regulation; Heterochromatin
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Law, P. (2016). Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals. (Doctoral Dissertation). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/236574
Chicago Manual of Style (16th Edition):
Law, Pui-pik. “Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals.” 2016. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
http://hdl.handle.net/10722/236574.
MLA Handbook (7th Edition):
Law, Pui-pik. “Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals.” 2016. Web. 26 Jan 2020.
Vancouver:
Law P. Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals. [Internet] [Doctoral dissertation]. University of Hong Kong; 2016. [cited 2020 Jan 26].
Available from: http://hdl.handle.net/10722/236574.
Council of Science Editors:
Law P. Investigation into the role of heterochromatin protein 1
gamma (HP1[gamma]) in gene regulation in mammals. [Doctoral Dissertation]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/236574
University of Hong Kong
26.
鄭楠; Zheng, Nan.
Identification and characterization of a
chloroacetate-inducible glycolate operon in Burkholderia caribensis
MBA4.
Degree: PhD, 2016, University of Hong Kong
URL: http://hdl.handle.net/10722/238836
► It has been confirmed that the ability of Burkholderia caribensis MBA4 in metabolizing halogenated organic compounds is due to a haloacid operon containing a dehalogenase…
(more)
▼ It has been confirmed that the ability of
Burkholderia caribensis MBA4 in metabolizing halogenated organic
compounds is due to a haloacid operon containing a dehalogenase
Deh4a and a haloacid transporterDeh4p. Haloacetates are transported
across the cell membrane with the assistance of Deh4p and degraded
by Deh4a to form glycolate. Previous studies showed that the
transcription of this haloacid operon is negatively regulated.
Furthermore, the binding of putative regulators to the promoter of
this operon is affected by monochloroacetate.
Previous studies
mainly focused on the dehalogenation mechanism of monochloroacetate
where glycolate is the metabolite. Glycolate is subsequently
catabolized by enzymes of the central metabolic pathways. In
Escherichia coli the transcription of a glycolate operon, encoding
for glycolate oxidase and malate synthase G, is induced by
glycolate. In MBA4 a glycolate operon was identified and which is
inducible by glycolate but much more by monochloroacetate. In
Escherichia coli the glycolate operon is regulated by GlcC. Similar
gene is also identified in MBA4. The aim of this study is to
characterize this chloroacetate-inducible glycolate operon.
Reverse-transcription PCR analysis showed that the glycolate operon
in MBA4 composed of glcDEFGB genes (K788_0009107, K788_0000598,
etc.). The regulator gene glcC (K788_0000597) is located
immediately upstream on the opposite strand of the glycolate
operon. Gene disruption analysis confirmed the activator role of
this GlcC. Sequencing and bioinformatic analyses have identified
the presence of two putative GlcC binding sites between glcC and
glcDEFGB. A distal site, located at the beginning of glcC, and a
proximal site, located upstream of glcDEFGB. The functional roles
of these sites were evaluated with 5’-deletion assays and
RegPrecise V3.3. Electrophoretic mobility shift assays implied the
binding of GlcC to these two sites. When glycolate was the growth
substrate, the distal site was occupied. The proximal site was
bound when monochloroacetate was used. Confirmation of the specific
binding of GlcC was conducted with cell extracts of glcC‾ mutant
and with cell extracts of E. coli BL21(DE3) pLysS producing GlcC.
Further analysis showed that monochloroacetate is required for the
switching of binding of GlcC from the distal to the proximal site.
This also implied that the binding specificity of this GlcC of MBA4
to the proximal site is chloroacetate-dependent.
In this study a
chloroacetate-inducible glc operon has been identified and
characterized in Burkholderia caribensis MBA4. The presence of this
operon enables the efficient metabolism of haloacetate to glycolate
then to metabolite that can be used by the central metabolic
pathways. This implied that MBA4 is a bacterium well adapted for
the degradation of haloacid.
published_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy
Subjects/Keywords: Genetic regulation; Transcription factors; Bacterial genetics
Record Details
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Share »
Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
鄭楠; Zheng, N. (2016). Identification and characterization of a
chloroacetate-inducible glycolate operon in Burkholderia caribensis
MBA4. (Doctoral Dissertation). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/238836
Chicago Manual of Style (16th Edition):
鄭楠; Zheng, Nan. “Identification and characterization of a
chloroacetate-inducible glycolate operon in Burkholderia caribensis
MBA4.” 2016. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
http://hdl.handle.net/10722/238836.
MLA Handbook (7th Edition):
鄭楠; Zheng, Nan. “Identification and characterization of a
chloroacetate-inducible glycolate operon in Burkholderia caribensis
MBA4.” 2016. Web. 26 Jan 2020.
Vancouver:
鄭楠; Zheng N. Identification and characterization of a
chloroacetate-inducible glycolate operon in Burkholderia caribensis
MBA4. [Internet] [Doctoral dissertation]. University of Hong Kong; 2016. [cited 2020 Jan 26].
Available from: http://hdl.handle.net/10722/238836.
Council of Science Editors:
鄭楠; Zheng N. Identification and characterization of a
chloroacetate-inducible glycolate operon in Burkholderia caribensis
MBA4. [Doctoral Dissertation]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/238836
University of Hong Kong
27.
Wang, Shimeng.
Characterization of a universal stress protein UspA1171
of Burkholderia caribensis MBA4.
Degree: M. Phil., 2016, University of Hong Kong
URL: http://hdl.handle.net/10722/249195
► Burkholderia caribensis MBA4 is able to utilize haloacetic acids as growth substrates. A key enzyme, dehalogenase Deh4a, that enables bioremediation of halogenated organic compounds, was…
(more)
▼ Burkholderia caribensis MBA4 is able to utilize
haloacetic acids as growth substrates. A key enzyme, dehalogenase
Deh4a, that enables bioremediation of halogenated organic
compounds, was isolated from MBA4. A haloacid operon, which
consists the structural genes for Deh4a and a transporter, Deh4p,
was identified and characterized. So far, the transcription of this
operon was found to regulate negatively. However, the mechanism has
not been fully understood. Recently, a protein designated as
TetR6620, encoded by gene K788_006620, has been found to bind to
DNA fragment containing 100-bp of upstream non-coding region of the
haloacid operon. Previous study showed that cell extracts prepared
from glycolate-grown MBA4 produced two retardation complexes, A
& B. When gene K788_006620 was disrupted in a mutant, Ins6620,
the formation of the retardation complex A was abolished. Moreover,
when purified recombinant TetR6620 with an N-terminal histidine-tag
was used in similar bandshift assay, at least two retardation
complexes were detected. The mobilities of these complexes are
different from that of complexes A & B identified previously.
When recombinant TetR6620 was supplemented to cell extracts of
glycolate-grown Ins6620, the formation of complex A was restored.
These suggest that while TetR6620 is able to bind to the upstream
non-coding region of the haloacid operon, it probably interacted
with another protein to perform its binding activity. By means of
pull-down experiment, a universal stress protein UspA1171, encode
by gene K788_001171, was envisaged to interact with TetR6620.
In this study, the role of UspA1171 was characterized. A
K788_001171 disruptant was constructed. Plasmid pKNOCK-Cm was used
as a backbone and a derivative containing part of K788_001171 was
used to disrupt gene K788_001171 in MBA4. If UspA1171 is a
repressor for the haloacid operon, then a disruptant will have a
relieved expression of Deh4a in glycolate-grown cells. Quantitative
RT-PCR will be used to determine the expression levels of deh4a.
The results showed that the slow migrating retardation complex A,
identified in wildtype MBA4, was not found in glycolate-grown
Ins6620. The gene K788_001171 has been amplified from the genome of
MBA4 and cloned into an E. coli expression vector pET-14b. The
plasmid was constructed and used to express UspA1171 in E. coli
BL21(DE3). The recombinant histidine-tagged UspA1171 was
over-expressed, purified and used in immobilized-metal affinity
chromatography (IMAC) to pull down proteins that might interact
with it. In addition, UspA1171 was used in BS3 crosslinking
experiments with TetR6620. Western blot analysis was used to verify
their interaction. The IMAC results showed that a 24 kDa protein
was successfully pulled down from glycolate-grown Ins1171 by
UspA1171 conjugated Ni-NTA agarose column. Previous results of
chemical crosslinking showed that TetR6620 can form dimer but there
is no evidence of UspA1171 capable of forming dimer. Nonetheless,
with the presence of UspA1171 and BS3 crosslinker, there…
Advisors/Committee Members: Wong, AOL, Tsang, JSH.
Subjects/Keywords: Genetic transcription - Regulation; Bacterial genetics; Transcription factors
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, S. (2016). Characterization of a universal stress protein UspA1171
of Burkholderia caribensis MBA4. (Masters Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/249195
Chicago Manual of Style (16th Edition):
Wang, Shimeng. “Characterization of a universal stress protein UspA1171
of Burkholderia caribensis MBA4.” 2016. Masters Thesis, University of Hong Kong. Accessed January 26, 2020.
http://hdl.handle.net/10722/249195.
MLA Handbook (7th Edition):
Wang, Shimeng. “Characterization of a universal stress protein UspA1171
of Burkholderia caribensis MBA4.” 2016. Web. 26 Jan 2020.
Vancouver:
Wang S. Characterization of a universal stress protein UspA1171
of Burkholderia caribensis MBA4. [Internet] [Masters thesis]. University of Hong Kong; 2016. [cited 2020 Jan 26].
Available from: http://hdl.handle.net/10722/249195.
Council of Science Editors:
Wang S. Characterization of a universal stress protein UspA1171
of Burkholderia caribensis MBA4. [Masters Thesis]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/249195
University of Hong Kong
28.
Cheng, Xiaoqing.
On construction and identification problems in
probabilistic Boolean networks.
Degree: PhD, 2016, University of Hong Kong
URL: http://hdl.handle.net/10722/233932
► In recent decades, rapidly evolving genomic technologies provide a platform for exploring the massive amount of genomic data. At the same time, it also triggers…
(more)
▼ In recent decades, rapidly evolving genomic
technologies provide a platform for exploring the massive amount of
genomic data. At the same time, it also triggers dramatic
development in systems biology. A number of mathematical models
have been proposed to understand the dynamical behavior of the
biological systems. Among them, Boolean Network (BN) and its
stochastic extension Probabilistic Boolean Network (PBN) have
attracted much attention.
Identification and construction
problems are two kinds of vital problems in studying the behavior
of a PBN. A novel problem of observability of singleton attractors
was firstly proposed, which was defined as identifying the minimum
number of consecutive nodes to discriminate different singleton
attractors. It may help in finding biomarkers for different disease
types, thus it plays a vital role in the study of signaling
networks. The observability of singleton attractor problem can be
solved in O(n) time, where n is the number of genes in a BN. Later,
the problem was extended to discriminating periodical attractors.
For the periodical case, one has to consider multiple time steps
and a new algorithm was proposed. Moreover, one may also curious
about identifying the minimum set of nodes that can determine
uniquely the attractor cycles from the others in the network, this
problem was also addressed.
In order to study realistic PBNs,
inference on the structure of PBNs from gene expression time series
data was investigated. The number of samples required to uniquely
determine the structure of a PBN was studied. Two models were
proposed to study different classes of PBNs. Using theoretical
analysis and computational experiments the structure of a PBN can
be exactly identified with high probability from a relatively small
number of samples for some classes of PBNs having bounded indegree.
Furthermore, it is shown that there exist classes of PBNs for which
it is impossible to uniquely determine their structure from samples
under these two models.
Constructing the structure of a PBN from
a given probability transition matrix is another key problem. A
projection-based gradient descent method was proposed for solving
huge size constrained least square problems. It is a matrixfree
iterative scheme for solving the minimizer of the captured problem.
A convergence analysis of the scheme is given, and the algorithm is
then applied to the construction of a PBN given its probability
transition matrix. Efficiency and effectiveness of the proposed
method are verified through numerical experiments.
Semi-tensor
product approach is another powerful tool in constructing of BNs.
However, to our best knowledge, there is no result on the
relationship of the structure matrix and transition matrix of a BN.
It is shown that the probability structure matrix and probability
transition matrix are similar matrices. Three main problems in PBN
were discussed afterward: dynamics, steady-state distribution and
the inverse problem. Numerical examples are provided to show the
validity of our proposed…
Subjects/Keywords: Algebra, Boolean; Genetic regulation - Mathematical models
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APA (6th Edition):
Cheng, X. (2016). On construction and identification problems in
probabilistic Boolean networks. (Doctoral Dissertation). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/233932
Chicago Manual of Style (16th Edition):
Cheng, Xiaoqing. “On construction and identification problems in
probabilistic Boolean networks.” 2016. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
http://hdl.handle.net/10722/233932.
MLA Handbook (7th Edition):
Cheng, Xiaoqing. “On construction and identification problems in
probabilistic Boolean networks.” 2016. Web. 26 Jan 2020.
Vancouver:
Cheng X. On construction and identification problems in
probabilistic Boolean networks. [Internet] [Doctoral dissertation]. University of Hong Kong; 2016. [cited 2020 Jan 26].
Available from: http://hdl.handle.net/10722/233932.
Council of Science Editors:
Cheng X. On construction and identification problems in
probabilistic Boolean networks. [Doctoral Dissertation]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/233932
University of Hong Kong
29.
Chen, Wei.
A factor analysis approach to transcription regulatory
network reconstruction using gene expression data.
Degree: PhD, 2012, University of Hong Kong
URL: Chen,
W.
[陈玮].
(2012).
A
factor
analysis
approach
to
transcription
regulatory
network
reconstruction
using
gene
expression
data.
(Thesis).
University
of
Hong
Kong,
Pokfulam,
Hong
Kong
SAR.
Retrieved
from
http://dx.doi.org/10.5353/th_b4961778
;
http://dx.doi.org/10.5353/th_b4961778
;
http://hdl.handle.net/10722/180958
► Reconstruction of Transcription Regulatory Network (TRN) and Transcription Factor Activity (TFA) from gene expression data is an important problem in systems biology. Currently, there exist…
(more)
▼ Reconstruction of Transcription Regulatory Network
(TRN) and Transcription Factor Activity (TFA) from gene expression
data is an important problem in systems biology. Currently, there
exist various factor analysis methods for TRN reconstruction, but
most approaches have specific assumptions not satisfied by real
biological data. Network Component Analysis (NCA) can handle such
limitations and is considered to be one of the most effective
methods. The prerequisite for NCA is knowledge of the structure of
TRN. Such structure can be obtained from ChIP-chip or ChIP-seq
experiments, which however have quite limited applications. In
order to cope with the difficulty, we resort to heuristic
optimization algorithm such as Particle Swarm Optimization (PSO),
in order to explore the possible structures of TRN and choose the
most plausible one. Regarding the structure estimation problem, we
extend classical PSO and propose a novel Probabilistic binary PSO.
Furthermore, an improved NCA called FastNCA is adopted to compute
the objective function accurately and fast, which enables PSO to
run efficiently. Since heuristic optimization cannot guarantee
global convergence, we run PSO multiple times and integrate the
results. Then GCV-LASSO (Generalized Cross Validation - Least
Absolute Shrinkage and Selection Operator) is performed to estimate
TRN. We apply our approach and other factor analysis methods on the
synthetic data. The results indicate that the proposed
PSOFastNCA-GCV-LASSO algorithm gives better estimation. In order to
incorporate more prior information on TRN structure and gene
expression dynamics in the linear factor analysis model for
improved estimation of TRN and TFAs, a linear Bayesian framework is
adopted. Under the unified Bayesian framework, Bayesian Linear
Sparse Factor Analysis Model (BLSFM) and Bayesian Linear State
Space Model (BLSSM) are developed for instantaneous and dynamic
TRN, respectively. Various approaches to incorporate partial and
ambiguous prior network structure information in the Bayesian
framework are proposed to improve performance in practical
applications. Furthermore, we propose a novel mechanism for
estimating the hyper-parameters of the distribution priors in our
BLSFM and BLSSM models, which can significantly improve the
estimation compared to traditional ways of hyper-parameter setting.
With this development, reasonably good estimation of TFAs and TRN
can be obtained even without use of any structure prior of TRN.
Extensive numerical experiments are performed to investigate our
developed methods under various settings, with comparison to some
existing alternative approaches. It is demonstrated that our
hyper-parameter estimation method improves the estimation of TFA
and TRN in most settings and has superior performance, and that
structure priors in general leads to improved estimation
performance. Regarding application to real biological data, we
execute the PSO-FastNCAGCV-LASSO algorithm developed in the thesis
using E. Coli microarray data and obtain sensible estimation of
TFAs and…
Advisors/Committee Members: Hung, YS, Chang, C.
Subjects/Keywords: Genetic transcription - Regulation - Statistical
methods.; Factor analysis.
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chen, W. (2012). A factor analysis approach to transcription regulatory
network reconstruction using gene expression data. (Doctoral Dissertation). University of Hong Kong. Retrieved from Chen, W. [陈玮]. (2012). A factor analysis approach to transcription regulatory network reconstruction using gene expression data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961778 ; http://dx.doi.org/10.5353/th_b4961778 ; http://hdl.handle.net/10722/180958
Chicago Manual of Style (16th Edition):
Chen, Wei. “A factor analysis approach to transcription regulatory
network reconstruction using gene expression data.” 2012. Doctoral Dissertation, University of Hong Kong. Accessed January 26, 2020.
Chen, W. [陈玮]. (2012). A factor analysis approach to transcription regulatory network reconstruction using gene expression data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961778 ; http://dx.doi.org/10.5353/th_b4961778 ; http://hdl.handle.net/10722/180958.
MLA Handbook (7th Edition):
Chen, Wei. “A factor analysis approach to transcription regulatory
network reconstruction using gene expression data.” 2012. Web. 26 Jan 2020.
Vancouver:
Chen W. A factor analysis approach to transcription regulatory
network reconstruction using gene expression data. [Internet] [Doctoral dissertation]. University of Hong Kong; 2012. [cited 2020 Jan 26].
Available from: Chen, W. [陈玮]. (2012). A factor analysis approach to transcription regulatory network reconstruction using gene expression data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961778 ; http://dx.doi.org/10.5353/th_b4961778 ; http://hdl.handle.net/10722/180958.
Council of Science Editors:
Chen W. A factor analysis approach to transcription regulatory
network reconstruction using gene expression data. [Doctoral Dissertation]. University of Hong Kong; 2012. Available from: Chen, W. [陈玮]. (2012). A factor analysis approach to transcription regulatory network reconstruction using gene expression data. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961778 ; http://dx.doi.org/10.5353/th_b4961778 ; http://hdl.handle.net/10722/180958
Montana State University
30.
Patterson, Kathryn Grace.
Gene regulation in the lac operon.
Degree: College of Letters & Science, 2009, Montana State University
URL: https://scholarworks.montana.edu/xmlui/handle/1/2024
► The lac operon, a jointly controlled series of genes in the bacteria E. coli, has been studied extensively since the 1940's. The lac operon genes…
(more)
▼ The lac operon, a jointly controlled series of genes in the bacteria E. coli, has been studied extensively since the 1940's. The lac operon genes are transcribed and then translated into proteins necessary for transport and digestion of lactose. The operon is activated in the presence of lactose after glucose, the preferred carbon source, has been expended. In this thesis, we introduce a biophysical model using the Shea-Ackers framework for modeling promoter dynamics. The model spans two scales: the inputs are biophysical parameters of molecular interactions and the result is a level of gene expression - a macroscopic behavior of the cell. We include all experimentally suggested control mechanisms into the model, even though the experimental evidence is stronger for some of these mechanisms than others. We compare our model to experimental data and explore the individual contribution of the proposed mechanisms by removing them one by one and testing the reduced model's fit to the data. Finally, we find a minimal model which faithfully represents the available data, yet includes only the minimal number of control mechanisms.
Advisors/Committee Members: Chairperson, Graduate Committee: Tomas Gedeon. (advisor).
Subjects/Keywords: Lac operon.; Biomathematics.; Genetic regulation.; Statistical thermodynamics.
Record Details
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Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Patterson, K. G. (2009). Gene regulation in the lac operon. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/2024
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Patterson, Kathryn Grace. “Gene regulation in the lac operon.” 2009. Thesis, Montana State University. Accessed January 26, 2020.
https://scholarworks.montana.edu/xmlui/handle/1/2024.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Patterson, Kathryn Grace. “Gene regulation in the lac operon.” 2009. Web. 26 Jan 2020.
Vancouver:
Patterson KG. Gene regulation in the lac operon. [Internet] [Thesis]. Montana State University; 2009. [cited 2020 Jan 26].
Available from: https://scholarworks.montana.edu/xmlui/handle/1/2024.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Patterson KG. Gene regulation in the lac operon. [Thesis]. Montana State University; 2009. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2024
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations
