subject:(GSH)

University of Hawaii – Manoa

1. Pang, Xiaosha. HIV-induced redox changes and inflammation in the central nervous system and modulatory factors.

Degree: 2016, University of Hawaii – Manoa

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Ph.D. University of Hawaii at Manoa 2013.

Currently 34 million people live with human immunodeficiency virus (HIV) infection. Although the lifespan of HIV patients has… (more)

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Ph.D. University of Hawaii at Manoa 2013.

Currently 34 million people live with human immunodeficiency virus (HIV) infection. Although the lifespan of HIV patients has been prolonged by highly active antiretroviral treatment, the prevalence of HIV-associated neurocognitive disorders (HANDs) is increasing. Oxidative stress and inflammation in the central nervous system (CNS) are major causes of HANDs, and concurrent drug abuse may accelerate the progression of symptoms. This dissertation aims to study HIV-induced changes in antioxidants, oxidative stress, and inflammation in the CNS, assess the effects of concurrent methamphetamine (Meth) exposure, and evaluate the protective effect of dietary supplementation of bamboo Phyllostachys edulis extract (BEX). This study centered on the most abundant antioxidant in the brain-glutathione (GSH). The changes of GSH metabolism and GSH-dependent antioxidant enzymes were measured in cerebrospinal fluid (CSF) of human cohorts with HIV infection and/or uses of Meth, and in the brain tissues of HIV-1 model transgenic rats (HIV-1Tg expressing 7 HIV viral proteins) with or without Meth exposure. HIV infection resulted in elevated gamma-glutamyl transpeptidase (GGT) activity, GSH depletion and a several-fold increase in lipid peroxidation in human CSF. Compared to HIV infection, use of Meth resulted in less severe oxidative stress, which may be partially explained by the upregulation of glutathione peroxidase (GPx) and GSH in the CSF. Meth did not interact with HIV in modulating the redox changes in the CSF. In HIV-1Tg rats, redox and inflammatory changes were observed in the brain in a region-specific manner, with little synergy from Meth exposure. The thalamus was highlighted by its high GSH content and systematic upregulation of GSH biosynthesis and GSH-dependent antioxidant enzymes in the HIV-1Tg rats. On the other hand, neuroinflammation markers, such as glial fibrillary acidic protein (GFAP) and p65, were increased in the hippocampus of the transgenic rats, and such changes were effectively normalized by dietary supplementation of BEX. In summary, this dissertation documents the significance of GSH and GSH-dependent antioxidant enzymes in regulating the redox status of CNS, and the potential anti-neuroinflammatory effects of BEX in the context of HIV infection.

Subjects/Keywords: glutathione (GSH)

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APA (6^th Edition):

Pang, X. (2016). HIV-induced redox changes and inflammation in the central nervous system and modulatory factors. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100634

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pang, Xiaosha. “HIV-induced redox changes and inflammation in the central nervous system and modulatory factors.” 2016. Thesis, University of Hawaii – Manoa. Accessed January 18, 2021. http://hdl.handle.net/10125/100634.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pang, Xiaosha. “HIV-induced redox changes and inflammation in the central nervous system and modulatory factors.” 2016. Web. 18 Jan 2021.

Vancouver:

Pang X. HIV-induced redox changes and inflammation in the central nervous system and modulatory factors. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10125/100634.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pang X. HIV-induced redox changes and inflammation in the central nervous system and modulatory factors. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/100634

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

2. Lo, An-ju. Effect of morin and GST on the growth and migration of MDA-435s cells.

Degree: Master, Biological Sciences, 2011, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0112111-204002

► The major functions of glutathione S-transferase (Glutathione S-transferase; GST) include the maintenance of intracellular redox state of substances, and detoxification of cells of endogenous and… (more)

Subjects/Keywords: SjGST; morin; GSH

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APA (6^th Edition):

Lo, A. (2011). Effect of morin and GST on the growth and migration of MDA-435s cells. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0112111-204002

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lo, An-ju. “Effect of morin and GST on the growth and migration of MDA-435s cells.” 2011. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0112111-204002.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lo, An-ju. “Effect of morin and GST on the growth and migration of MDA-435s cells.” 2011. Web. 18 Jan 2021.

Vancouver:

Lo A. Effect of morin and GST on the growth and migration of MDA-435s cells. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0112111-204002.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lo A. Effect of morin and GST on the growth and migration of MDA-435s cells. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0112111-204002

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Tarhan, Seda. ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi .

Degree: ESOGÜ, Tıp Fakültesi, Tıbbi Biyokimya, 2013, Eskisehir Osmangazi University

URL: http://hdl.handle.net/11684/691

► Bu çalışmada ε-viniferinin tek başına ve vinkristin sülfat kombine uygulamasının HepG2 hücrelerinde lipid peroksidasyonu (LPO), süperoksit dismutaz (SOD) ve redükte glutatyon (GSH) düzeylerine doğrudan ve… (more)

▼ Bu çalışmada ε-viniferinin tek başına ve vinkristin sülfat kombine uygulamasının HepG2 hücrelerinde lipid peroksidasyonu (LPO), süperoksit dismutaz (SOD) ve redükte glutatyon (GSH) düzeylerine doğrudan ve H2O2 ile dışsal kaynaklı oluşturulmuş oksidatif stres üzerine koruyucu etkilerinin araştırılması amaçlanmıştır. Çalışmamız birinci aşamada, maddelerin düşük ve yüksek dozlarından oluşturulmuş dVNF, yVNF, dVCR, yVCR, dETO, yETO, d(VCR+VNF), y(VCR+VNF) grupları ile Kontrol grubundan ve ikinci aşamada bu gruplara ek olarak dışsal kaynaklı oksidatif strese karşı maddelerin koruyucu etkilerini gözlemleyebilmek için kullanılan H2O2-Kontrol grubundan oluşturulmuştur. Çalışmamızda sıvı azot tankından çıkarılan hücreler besiyerinde çoğaltılmıştır. Hücreler yeterli sayıya ulaştıktan sonra maddelerin düşük ve yüksek dozları ile 3, 6 ve 24 saat inkübe edilmişlerdir. Antioksidan özelliklerini incelediğimiz maddelerin LPO ve SOD ölçümleri deney kitleri ile kolorometrik olarak ölçülmüş sonuçlar absorbansa karşı değerlendirilmiştir. GSH ölçümleri ise flourometrik deney kiti ile ölçülerek sonuçlar floresans ışımasına karşı değerlendirilmiştir. Sonuç olarak, birinci aşamada vinkristin sülfat zamana ve doza bağımlı olarak LPO SOD ve GSH değerleri üzerinde kontrole göre anlamlı bir etki göstermemiştir. ε-viniferin ile kombine uyguladığımız ise LPO ve GSH düzeylerinde kontrole göre anlamlı bir etki göstermemiş ancak SOD aktivasyonunu kontrole göre anlamlı olarak azaltmıştır (p<0,05). Elde ettiğimiz verilere göre, zamana bağımlı olarak kombine uygulamanın HepG2 hücrelerinde SOD aktivasyonunu azaltarak hücresel oksidatif stresi arttırabileceğini ve hepatoma hücrelerinde hasar yaratacağını düşünüyoruz.İkinci aşamada ise düşük doz kombine uygulamanın tüm parametrelerde artışa yol açarken, yüksek doz kombine uygulamanın H2O2 ile uyarılmış LPO düzeylerini etkilemeden SOD aktivasyonunu ve GSH düzeylerini arttırması kombine uygulamanın HepG2 hücreleri üzerinde dışsal oksidatif strese karşı koruyucu etkisi olduğunu göstermektedir. Advisors/Committee Members: Sütken Demirkan, Emine (advisor).

Subjects/Keywords: ε-viniferin; HepG2; LPO; SOD; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tarhan, S. (2013). ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi . (Thesis). Eskisehir Osmangazi University. Retrieved from http://hdl.handle.net/11684/691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tarhan, Seda. “ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi .” 2013. Thesis, Eskisehir Osmangazi University. Accessed January 18, 2021. http://hdl.handle.net/11684/691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tarhan, Seda. “ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi .” 2013. Web. 18 Jan 2021.

Vancouver:

Tarhan S. ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi . [Internet] [Thesis]. Eskisehir Osmangazi University; 2013. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11684/691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tarhan S. ε-Viniflerinin tek başına ve kemoterapötik bir ajan ile birlikte HEPG2 hücreleri üzerine antioksidan etkisinin incelenmesi . [Thesis]. Eskisehir Osmangazi University; 2013. Available from: http://hdl.handle.net/11684/691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

4. Lee, John P. TRPM2 Inhibition Increases Oxidative Stress and Reduces Tumor Growth in Neuroblastoma through Modulation of GSH.

Degree: 2017, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/14004jpl5224

► The transient receptor potential melastatin 2 (TRPM2) ion channel modulates cell signaling pathways in response to oxidative stress and is highly expressed in neuroblastoma and… (more)

Subjects/Keywords: TRPM2; Nrf2; Glutathione; GSH; Oxidative stress; Neuroblastoma

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APA (6^th Edition):

Lee, J. P. (2017). TRPM2 Inhibition Increases Oxidative Stress and Reduces Tumor Growth in Neuroblastoma through Modulation of GSH. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/14004jpl5224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, John P. “TRPM2 Inhibition Increases Oxidative Stress and Reduces Tumor Growth in Neuroblastoma through Modulation of GSH.” 2017. Thesis, Penn State University. Accessed January 18, 2021. https://submit-etda.libraries.psu.edu/catalog/14004jpl5224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, John P. “TRPM2 Inhibition Increases Oxidative Stress and Reduces Tumor Growth in Neuroblastoma through Modulation of GSH.” 2017. Web. 18 Jan 2021.

Vancouver:

Lee JP. TRPM2 Inhibition Increases Oxidative Stress and Reduces Tumor Growth in Neuroblastoma through Modulation of GSH. [Internet] [Thesis]. Penn State University; 2017. [cited 2021 Jan 18]. Available from: https://submit-etda.libraries.psu.edu/catalog/14004jpl5224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee JP. TRPM2 Inhibition Increases Oxidative Stress and Reduces Tumor Growth in Neuroblastoma through Modulation of GSH. [Thesis]. Penn State University; 2017. Available from: https://submit-etda.libraries.psu.edu/catalog/14004jpl5224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

5. Kim, Maya Hwewon. Glutaredoxin-1 regulates the Keap1-Nrf2 pathway.

Degree: MS, Pathology, 2017, Boston University

URL: http://hdl.handle.net/2144/26675

► PURPOSE: The Nrf2/Keap1/ARE pathway is a major regulator of cytoprotective responses to oxidants. Gluatredoxin-1 (Glrx-1), a small thiol transferase removes glutathione (GSH) adducts from proteins… (more)

Subjects/Keywords: Medicine; Glrx; GSH; Keap1; NAFLD; NASH; Nrf2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, M. H. (2017). Glutaredoxin-1 regulates the Keap1-Nrf2 pathway. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/26675

Chicago Manual of Style (16^th Edition):

Kim, Maya Hwewon. “Glutaredoxin-1 regulates the Keap1-Nrf2 pathway.” 2017. Masters Thesis, Boston University. Accessed January 18, 2021. http://hdl.handle.net/2144/26675.

MLA Handbook (7^th Edition):

Kim, Maya Hwewon. “Glutaredoxin-1 regulates the Keap1-Nrf2 pathway.” 2017. Web. 18 Jan 2021.

Vancouver:

Kim MH. Glutaredoxin-1 regulates the Keap1-Nrf2 pathway. [Internet] [Masters thesis]. Boston University; 2017. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2144/26675.

Council of Science Editors:

Kim MH. Glutaredoxin-1 regulates the Keap1-Nrf2 pathway. [Masters Thesis]. Boston University; 2017. Available from: http://hdl.handle.net/2144/26675

6. Cruzat, Vinicius Fernandes. Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia.

Degree: PhD, Nutrição Experimental, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/9/9132/tde-23052013-152405/ ;

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A sepse é a principal causa de morte em unidades de terapia intensiva (UTIs) no mundo. A reduzida disponibilidade do aminoácido mais abundante do organismo,… (more)

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A sepse é a principal causa de morte em unidades de terapia intensiva (UTIs) no mundo. A reduzida disponibilidade do aminoácido mais abundante do organismo, a glutamina contribui para o complicado estado catabólico da sepse. No presente estudo investigamos os efeitos da suplementação oral com L-glutamina e L-alanina (GLN+ALA), ambos na norma livre e como dipeptídeo, L-alanil-L-glutamina (DIP), sobre o eixo glutamina-glutationa (GSH), sistema imune, inflamação, proteínas de choque térmico (HSPs) e expressão de genes envolvidos com vias de sinalização proteica em animais endotoxêmicos. Camundongos C57/B6 foram submetidos à endotoxemia (Escherichia coli LPS, 5 mg.kg-1, grupo LPS) e suplementados por 48 horas com L-glutamina (1 g.kg-1) e L-alanina (0,61 g.kg-1, grupo GLN+ALA-LPS) ou 1,49 g.kg-1 de DIP (grupo DIP-LPS). A endotoxemia promoveu depleção da concentração de glutamina no plasma (71%), músculo esquelético (50%) e fígado (49%), quando comparado ao grupo CTRL, sendo restauradas nos grupos DIP-LPS e GLN+ALA-LPS (P<0,05), fato que atenuou a redução da GSH e o estado redox (taxa GSSG/GSH) em eritrócitos circulantes, musculo e fígado (P<0,05). A suplementação em animais endotoxêmicos resultou em uma upregulation dos genes GSR, GPX1 e GCLC no músculo e fígado. A concentração das citocinas plasmáticasTNF-α, IL-6, IL-1β e IL-10 foi atenuada pelas suplementações, bem como a expressão de mRNAs envolvidos com a resposta inflamatória, ativadas pela via do NF-κB(P<0,05). Concomitantemente, verificou-se aumento da capacidade proliferativa de linfócitos T e B circulantes nos grupos GLN+ALA-LPS e DIP-LPS. A expressão de mRNAs e a concentração de HSPs no tecido muscular foi restabelecida pelas suplementações, contudo, a expressão mRNAs relacionados às vias de síntese e degradação proteica foi somente estimulada no tecido hepático(P<0,05). Os resultados do presente estudo demonstram que a suplementação por via oral com GLN+ALA ou DIP podem ser utilizados clinicamente como métodos nutricionais em reverter o quadro de depressão da disponibilidade de glutamina corporal da sepse induzida por LPS, tendo impacto no eixo glutamina-glutationa, sistema imune e inflamatório.

Sepsis is the leading cause of death inintensive care units (ICUs) in the world.The availability ofthe most abundant amino acid in the body, glutamine, is reduced in this situation, fact that contribute to the complicated catabolic state of sepsis. In the present study, we investigated the effects of oral supplementation with L-glutamine and L-alanine (GLN+ALA), both in their free form and as a dipeptide, L-alanyl-L-glutamine (DIP) on glutamine-glutathione axis (GSH), immune and inflammatory system, heat shock proteins (HSPs) expression and gene expressions involved in protein signaling pathways during endotoxemia. C57/B6 mice were subjected to endotoxemia (Escherichia coli LPS, 5 mg.kg-1, LPS group) and supplemented for 48 hours with L-glutamine (1 g.kg-1) plus L-alanine(0.61 g.kg-1, GLN+ALA-LPS group) or 1.49 g.kg-1of DIP (DIP-LPS group). Endotoxemia…

Advisors/Committee Members: Bittencourt Junior, Paulo Ivo Homem de, Tirapegui, Julio.

Subjects/Keywords: Glutamina; Glutamine; GSH; GSH; HSP; HSP; NF-B; NF-B; Sepse; Sepsis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cruzat, V. F. (2013). Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/9/9132/tde-23052013-152405/ ;

Chicago Manual of Style (16^th Edition):

Cruzat, Vinicius Fernandes. “Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia.” 2013. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/9/9132/tde-23052013-152405/ ;.

MLA Handbook (7^th Edition):

Cruzat, Vinicius Fernandes. “Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia.” 2013. Web. 18 Jan 2021.

Vancouver:

Cruzat VF. Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/9/9132/tde-23052013-152405/ ;.

Council of Science Editors:

Cruzat VF. Efeito da suplementação com L-glutamina livre e na forma de dipeptídeo sobre eixo glutamina-glutationa, sistema imune, sistema inflamatório e vias de sinalização proteica em camundongos submetidos à endotoxemia. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/9/9132/tde-23052013-152405/ ;

7. Čijauskaitė, Kristina. Cinko ir selenito jonų įtaka redukuoto glutationo koncentracijai ir lipidų peroksidacijai kadmiu paveiktų laboratorinių pelių kepenyse.

Degree: Master, Pharmacy, 2014, Lithuanian Academic Libraries Network (LABT)

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215121-90540 ;

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Buvo nustatyta, kad redukuoto glutationo koncentraciją pelių kepenyse kadmis padidino po 8 val. 35 proc., o po 14 dienų sumažino 35 proc. Po 8 val.,… (more)

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Buvo nustatyta, kad redukuoto glutationo koncentraciją pelių kepenyse kadmis padidino po 8 val. 35 proc., o po 14 dienų sumažino 35 proc. Po 8 val., tiek cinkas, tiek selenas taip pat padidino redukuoto glutationo koncentraciją, atitinkamai, 27 proc. ir 17 proc. Įvertinant malondialdehido koncentraciją pelių kepenyse, buvo nustatyta, kad kadmis padidino malondialdehido koncentraciją po 8, 24 val. ir 14 dienų, atitinkamai, 336 proc., 218 proc. ir 182 proc. Veikiant cinkui ir selenui, malondialdehido koncentracija pelių kepenyse padidėjo po 24 val. ir 14 dienų, atitinkamai, 325 proc. ir 437 proc., o po 14 dienų, atitinkamai, 162 proc. ir 288 proc. Taigi, cinkas pajėgus apsaugoti redukuotą glutationą nuo oksidacijos tik 8 val., o po ilgesnio laiko redukuotas glutationas išeikvojamas. Po 8 ir 24 val. tiek cinkas, tiek selenas pelių kepenyse geba apsaugoti lipidus nuo peroksidacijos, o po 14 dienų redukuotą glutationą nuo oksidacijos ir lipidus nuo peroksidacijos apsaugo tik žaliosios arbatos ekstraktas.

It was determined, that after 8 h cadmium increased glutathione concentration by 35 % while after 14 days decreased by 35 %. After 8 h both zinc and selenium also increased reduced glutathione concentration, respectively, 27 % and 17 %. Evaluating malondialdehyde concentration in mice liver, it was established, that cadmium increased malondialdehyde concentration after 8, 24 h and 14 days, respectively, 336 %, 218 % and 182 %. When mice liver were affected with zinc and selenium, malondialdehyde concentration increased after 24 h, respectively, 325 % and 437 % and after 14 days, respectively, 162 % and 288 %. To sum up, zinc can protect reduced glutathione from oxidation in mice liver just for 8 h, and after a longer period reduced glutathione is depleted. After 8, 24 h and 14 days both, zinc and selenium are eager to protect liver from lipid peroxidation and after 14 days reduced glutathione from oxidation. Lipids from peroxidation process can protect only green tea extract.

Advisors/Committee Members: Bernotienė, Rasa (Master’s thesis supervisor), Ivanov, Leonid (Master’s thesis advisor), Savickienė, Nijolė (Master’s degree committee chair), Sadauskienė, Ilona (Master’s thesis reviewer), Rodovičius, Hiliaras (Master’s degree committee member), Dagilytė, Audronė (Master’s degree session secretary), Ivanauskas, Liudas (Master’s degree committee member), Savickas, Arūnas (Master’s degree committee member), Briedis, Vitalis (Master’s degree committee member), Ramanauskienė, Kristina (Master’s degree committee member), Inkėnienė, Asta Marija (Master’s degree committee member), Jakštas, Valdas (Master’s degree committee member), Drakšienė, Gailutė (Master’s degree committee member), Radžiūnas, Raimundas (Master’s degree committee member), Skyrius, Vaidas (Master’s degree committee member), Brusokas, Valdemaras (Master’s degree committee member), Barsteigienė, Zita (Master’s degree committee member), Marksienė, Rūta (Master’s degree committee member), Ževžikovas, Andrejus (Master’s degree committee member), Vitkevičius, Konradas (Master’s degree committee member), Gumbrevičius, Gintautas (Master’s degree committee member), Ragažinskienė, Onutė (Master’s degree committee member), Maruška, Audrius (Master’s degree committee member), Martinėnas, Žydrūnas (Master’s degree committee member), Kuncaitė, Giedrė (Master’s degree committee member), Zulaniene, Eglė Audronė (Master’s degree committee member), Jakubauskas, Mindaugas (Master’s degree committee member), Budrikienė, Aušra (Master’s degree committee member), Švarcaitė, Jūratė (Master’s degree committee member), Balanaškienė, Rima (Master’s degree committee member), Marcinkevičienė, Rasa (Master’s degree committee member).

Subjects/Keywords: Cinkas; Selenas; Žaliosios arbatos ekstraktas; GSH; MDA; Zinc; Selenium; GTE; GSH; MDA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Čijauskaitė, Kristina. (2014). Cinko ir selenito jonų įtaka redukuoto glutationo koncentracijai ir lipidų peroksidacijai kadmiu paveiktų laboratorinių pelių kepenyse. (Masters Thesis). Lithuanian Academic Libraries Network (LABT). Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215121-90540 ;

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Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Čijauskaitė, Kristina. “Cinko ir selenito jonų įtaka redukuoto glutationo koncentracijai ir lipidų peroksidacijai kadmiu paveiktų laboratorinių pelių kepenyse.” 2014. Masters Thesis, Lithuanian Academic Libraries Network (LABT). Accessed January 18, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215121-90540 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Čijauskaitė, Kristina. “Cinko ir selenito jonų įtaka redukuoto glutationo koncentracijai ir lipidų peroksidacijai kadmiu paveiktų laboratorinių pelių kepenyse.” 2014. Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Čijauskaitė, Kristina. Cinko ir selenito jonų įtaka redukuoto glutationo koncentracijai ir lipidų peroksidacijai kadmiu paveiktų laboratorinių pelių kepenyse. [Internet] [Masters thesis]. Lithuanian Academic Libraries Network (LABT); 2014. [cited 2021 Jan 18]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215121-90540 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Čijauskaitė, Kristina. Cinko ir selenito jonų įtaka redukuoto glutationo koncentracijai ir lipidų peroksidacijai kadmiu paveiktų laboratorinių pelių kepenyse. [Masters Thesis]. Lithuanian Academic Libraries Network (LABT); 2014. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2014~D_20140618_215121-90540 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

8. Cruzat, Vinicius Fernandes. Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação.

Degree: Mestrado, Nutrição Experimental, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/9/9132/tde-29012009-185922/ ;

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A glutamina é o aminoácido livre mais abundante no plasma e músculo, sendo utilizada em elevadas taxas por diversas células na manutenção e promoção de… (more)

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A glutamina é o aminoácido livre mais abundante no plasma e músculo, sendo utilizada em elevadas taxas por diversas células na manutenção e promoção de funções essenciais à homeostasia celular. Entretanto, em algumas situações catabólicas, entre as quais exercícios físicos intensos e prolongados ou exaustivos é observada a redução na concentração de glutamina, o que pode comprometer as funções celulares. A suplementação com dipeptídeos de glutamina, tais como a L-alanil-L-glutamina (DIP) pode representar um eficiente meio de fornecimento de glutamina por via oral para o organismo. Desta forma, ratos Wistar machos foram treinados e suplementados com o DIP (1,49 g•kg-1) ou uma solução contendo os aminoácidos L-glutamina (1 g•kg-1) e L-alanina (0,61 g•kg-1) livres (GLN+ALA) ou água (CON), sendo sacrificados em dois tempos, antes (TR) e após (INT) um exercício intenso de natação. No plasma, a concentração de glutamina, glutamato, glicose, amônia, CK, LDH, TNF-α e PgE2 foram avaliadas. No músculo sóleo e gastrocnêmio e no fígado foram determinadas as concentrações de glutamina, glutamato, GSH, GSSG e proteínas totais. Antes do exercício intenso, ambas as suplementações atenuaram a liberação de CK no plasma. O grupo DIP-TR apresentou maior concentração de glutamato, GSH e GSH/GSSG no sóleo e fígado. Maior concentração de glutamina, glutamato, GSH ou GSH/GSSG foi observada no sóleo e fígado do grupo GLN+ALA-TR. Após o exercício intenso, ambas as suplementações atenuaram a liberação de amônia e TNF-α, e no grupo DIP-INT menor concentração de PgE2 foi observada. Maior concentração de glutamina, glutamato e GSH/GSSG no sóleo e glutamato no gastrocnêmio foram encontrados nos grupos DIP-INT e GLN+ALA-INT. No fígado foi encontrada também maior concentração de GSH e GSH/GSSG, decorrentes das suplementações. Os nossos resultados levam à conclusão de que a suplementação oral com L-glutamina e L-alanina na forma livre ou como dipeptídeo, L-alanil-L-glutamina, pode aumentar os estoques musculares e hepáticos de GSH e alterar o estado redox celular, atenuando lesão e a inflamação induzidas pelo exercício físico.

Glutamine is the most abundant free amino acid found in plasma and muscle, and is utilized at high rates by many cells for maintenance and promotion of cell function. However, in some catabolic situations, like intense and prolonged or exhaustive physical exercise, a reduction in GLN availability is observed, fact that may impair the cell functions. Oral supplementation with dipeptides of glutamine, like L-alanyl-L-glutamine may serve as an interesting way to deliver glutamine to the organism. Male Wistar rats were trained and supplemented with DIP (1,49 g•kg-1) or mixture containing free L-glutamine (1 g•kg-1) and Lalanine (0,61 g•kg-1) or water (CON) and sacrificed in distinct times: before (TR) and immediately after (INT) intense exercise of swim. Plasma and serum concentrations of glucose, GLN, glutamate, ammonia, CK, LDH, TNF-• and PgE2 were evaluated. In muscles…

Advisors/Committee Members: Tirapegui, Julio.

Subjects/Keywords: Dipeptide; Dipeptídeo; Glutamina; Glutamine; GSH; GSH; Inflamação; Inflammation; Lesão muscular; Muscle damage; Nutrição experimental; Suplementação alimentar

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cruzat, V. F. (2008). Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/9/9132/tde-29012009-185922/ ;

Chicago Manual of Style (16^th Edition):

Cruzat, Vinicius Fernandes. “Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação.” 2008. Masters Thesis, University of São Paulo. Accessed January 18, 2021. http://www.teses.usp.br/teses/disponiveis/9/9132/tde-29012009-185922/ ;.

MLA Handbook (7^th Edition):

Cruzat, Vinicius Fernandes. “Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação.” 2008. Web. 18 Jan 2021.

Vancouver:

Cruzat VF. Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação. [Internet] [Masters thesis]. University of São Paulo; 2008. [cited 2021 Jan 18]. Available from: http://www.teses.usp.br/teses/disponiveis/9/9132/tde-29012009-185922/ ;.

Council of Science Editors:

Cruzat VF. Efeito da suplementação com L-glutamina e L-alanil-L-glutamina sobre parâmetros de lesão muscular e de inflamação em ratos treinados e submetidos a exercício intenso de natação. [Masters Thesis]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/9/9132/tde-29012009-185922/ ;

Universidade Federal de Santa Maria

9. Viviane Patrícia Pires Schiar. AVALIAÇÃO TOXICOLÓGICA DE ORGANOCALCOGÊNIOS EM ERITRÓCITOS HUMANOS.

Degree: 2009, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2650

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Nos últimos anos, os compostos organocalcogênios têm sido alvos de interesse em síntese orgânica em virtude da descoberta de suas aplicações industriais e de suas… (more)

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Nos últimos anos, os compostos organocalcogênios têm sido alvos de interesse em síntese orgânica em virtude da descoberta de suas aplicações industriais e de suas propriedades farmacológicas. No entanto, dados da literatura têm demonstrado que estes compostos apresentam atividade pró-oxidante ocasionando danos teciduais e inibição da atividade de enzimas. Sabe-se que o estresse oxidativo pode causar alterações em proteínas da membrana de eritrócitos, logo estas células representam um bom modelo para investigar danos induzidos por agentes oxidantes. Desta forma, no presente trabalho investigou-se a toxicidade de uma variedade de calcogênios sobre eritrócitos in vitro nas concentrações de 10, 40, 100 e 200 μM. Os resultados deste trabalho mostraram que para um hematócrito de 45% apenas os compostos de organotelúrio foram tóxicos causando hemólise e estes efeitos não foram correlacionados com a produção de substâncias reativas ao ácido tiobarbitúrico, sugerindo deste modo não haver relação com a peroxidação lipídica. No entanto, utilizou-se um hematócrito de 1% e os compostos de organoselênio e organotelúrio testados apresentaram toxicidade causando hemólise. Esta hemólise foi relacionada com a oxidação da glutationa intracelular (GSH) e uma possível produção de radicais livres. Portanto, os resultados apresentados nesta tese sugerem que os compostos orgânicos contendo selênio ou telúrio apresentaram toxicidade para os eritrócitos.

The interest in organochalcogen chemistry, biochemistry and pharmacology has increased in the last two decades mainly due to the fact that a variety of organochalcogen compounds possess biological activity and due the use of these compounds in industrial applications. However, literature data showed that these compounds present pro-oxidant properties, causing tissue damage and inhibition a variety of enzymes. Oxidative stress can induce complex alterations of membrane proteins in erythrocytes. Erythrocytes represent a good model to investigate the damage induced by oxidizing agents. Therefore, the aim of the present study was to evaluate the toxicity induced by a variety of chalcogens at concentrations of 10, 40, 100 e 200 μM using erythrocytes in vitro. The present results showed that organotellurium compounds were toxic to erythrocytes for a hematocrit of 45%. The hemolytic effect of tellurides was not positively correlated with thiobarbituric acidreactive substance (TBARS) production suggesting that lipid peroxidation is not involved in the hemolysis provoked by organotellurium compounds. However, for a hematocrit of 1% the organoselenium and organotellurium compounds increased the hemolysis rate and these results suggest a relationship between the oxidation of intracellular glutathione (GSH) and subsequent generation of free radicals with the hemolysis by chalcogen compounds. Therefore, the results presented in this study suggest that organochalcogen compounds presented toxicity for the erythrocytes.

Advisors/Committee Members: João Batista Teixeira da Rocha, Gilson Rogério Zeni, Eder Joao Lenardao, Lisiane de Oliveira Porciúncula.

Subjects/Keywords: oxidação; GSH; peroxidação lipídica; hemólise; eritrócitos; organotelúrio; organosselênio; organocalcogênios; BIOQUIMICA; organotellurium; organoselenium; organochalcogens; erythrocytes; hemolysis; lipid peroxidation; GSH; oxidation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schiar, V. P. P. (2009). AVALIAÇÃO TOXICOLÓGICA DE ORGANOCALCOGÊNIOS EM ERITRÓCITOS HUMANOS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schiar, Viviane Patrícia Pires. “AVALIAÇÃO TOXICOLÓGICA DE ORGANOCALCOGÊNIOS EM ERITRÓCITOS HUMANOS.” 2009. Thesis, Universidade Federal de Santa Maria. Accessed January 18, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schiar, Viviane Patrícia Pires. “AVALIAÇÃO TOXICOLÓGICA DE ORGANOCALCOGÊNIOS EM ERITRÓCITOS HUMANOS.” 2009. Web. 18 Jan 2021.

Vancouver:

Schiar VPP. AVALIAÇÃO TOXICOLÓGICA DE ORGANOCALCOGÊNIOS EM ERITRÓCITOS HUMANOS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2009. [cited 2021 Jan 18]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schiar VPP. AVALIAÇÃO TOXICOLÓGICA DE ORGANOCALCOGÊNIOS EM ERITRÓCITOS HUMANOS. [Thesis]. Universidade Federal de Santa Maria; 2009. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=2650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

10. Emmanuel Veiga de Camargo. ATIVIDADE DE NEUTROFILOS E ESTRESSE OXIDATIVO EM CORDEIROS INFECTADOS EXPERIMENTALMENTE COM Haemonchus contortus E SUPLEMENTADOS COM SELÊNIO E VITAMINA E.

Degree: 2010, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3008

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O presente trabalho teve como objetivo avaliar o metabolismo oxidativo dos neutrófilos, o hemograma e o perfil oxidativo de cordeiros experimentalmente infectados com Haemonchus contortus… (more)

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O presente trabalho teve como objetivo avaliar o metabolismo oxidativo dos neutrófilos, o hemograma e o perfil oxidativo de cordeiros experimentalmente infectados com Haemonchus contortus e suplementados com selênio e vitamina E. Foram utilizados 20 cordeiros machos, da raça Corriedale, distribuídos em quatro grupos experimentais com 5 animais: G1- animais infectados com larvas e suplementados com 0,2mg/kg de peso vivo (PV) de selenito de sódio por via intramuscular (IM); G2- animais infectados com larvas e suplementados com 0,2mg/kg PV de selenito de sódio IM e 2000 UI por animal de Vitamina E IM; G3- animais infectados com larvas e suplementados com 2000 UI por animal de Vitamina E IM; G4-animais infectados com larvas. Todos os grupos foram infectados, pela via oral, com 500 larvas L3 de Haemonchus contortus por animal a cada dois dias, pelo período de vinte dias a partir do dia zero. Para o hemograma e as análises bioquímicas foram realizadas coletas de sangue nos dias zero (T0), 20 (T1), 40 (T2) e 60 (T3) por venopunção da jugular utilizando-se tubos vacutainer. As pesagens e a determinação dos ovos por grama de fezes dos animais ocorreram nesses mesmos tempos experimentais. Para as provas de NBT foram coletadas amostras de sangue heparinizadas nos dias zero, 30 e 60. Menores valores de leucócitos totais foram detectados no grupo suplementado exclusivamente com selênio (G1) em relação ao grupo controle (G4) no tempo 4. Em relação aos linfócitos observou-se diminuição no G1 em relação ao suplementado somente com vitamina E (G3) e G4 no tempo 3 (T3). Para ambos os testes, NBT-NE e NBT-E houve uma diminuição da capacidade de redução do corante aos 60 dias em relação aos demais tempos nos grupos tratados com selênio (G1 e G2).Os resultados do perfil oxidativo demonstraram significativa elevação da atividade da enzima GSH-Px nos grupos suplementados com selênio. Ainda, a correlação de Pearson revelou a existência de correlação negativa entre as concentrações de GSH-Px e TBARS e entre esta enzima e os valores de OPG. Incrementos também foram observados para a enzima catalase nos animais que receberam suplementação com selênio ou quando este elemento foi associado a vitamina E. Menores valores de lipoperoxidação lipídica (TBARS) foram detectados nos animais suplementados quando estes foram comparados ao grupo controle. Esses resultados sugerem que as reservas diminutas deste antioxidante podem exacerbar a geração de radicais livres com um aumento da peroxidação lipídica e o aumento da contaminação ambiental pelos ovos de Haemonchus contortus. Diante dos resultados é possível concluir que a suplementação com selênio proporciona uma maior proteção antioxidante celular e ao organismo como um todo de cordeiros experimentalmente infectados pelo Haemonchus contortus.

The present work had as its objective to assess the oxidizing metabolism of neutrophils, the complete blood count (CBC) and the oxidizing profile of experimentally infected lambs with Haemonchus contortus and supplemented with selenium and vitamin E. 20 male…

Advisors/Committee Members: Marcelo Soares, Sonia Teresinha dos Anjos Lopes, Marta Lizandra do Rêgo Leal.

Subjects/Keywords: suplementação; TBARS; GSH-Px; hemograma; função neutrofílica; cordeiros; MEDICINA VETERINARIA; complete blood count; neutrophil function; lambs; GSH-Px; TBARS; supplementation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Camargo, E. V. d. (2010). ATIVIDADE DE NEUTROFILOS E ESTRESSE OXIDATIVO EM CORDEIROS INFECTADOS EXPERIMENTALMENTE COM Haemonchus contortus E SUPLEMENTADOS COM SELÊNIO E VITAMINA E. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Camargo, Emmanuel Veiga de. “ATIVIDADE DE NEUTROFILOS E ESTRESSE OXIDATIVO EM CORDEIROS INFECTADOS EXPERIMENTALMENTE COM Haemonchus contortus E SUPLEMENTADOS COM SELÊNIO E VITAMINA E.” 2010. Thesis, Universidade Federal de Santa Maria. Accessed January 18, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Camargo, Emmanuel Veiga de. “ATIVIDADE DE NEUTROFILOS E ESTRESSE OXIDATIVO EM CORDEIROS INFECTADOS EXPERIMENTALMENTE COM Haemonchus contortus E SUPLEMENTADOS COM SELÊNIO E VITAMINA E.” 2010. Web. 18 Jan 2021.

Vancouver:

Camargo EVd. ATIVIDADE DE NEUTROFILOS E ESTRESSE OXIDATIVO EM CORDEIROS INFECTADOS EXPERIMENTALMENTE COM Haemonchus contortus E SUPLEMENTADOS COM SELÊNIO E VITAMINA E. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2010. [cited 2021 Jan 18]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Camargo EVd. ATIVIDADE DE NEUTROFILOS E ESTRESSE OXIDATIVO EM CORDEIROS INFECTADOS EXPERIMENTALMENTE COM Haemonchus contortus E SUPLEMENTADOS COM SELÊNIO E VITAMINA E. [Thesis]. Universidade Federal de Santa Maria; 2010. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Farida, Siti. Influence de l'environnement (exercice et nutrition) durant la gestation sur l’état de stress oxydant et le métabolisme du glucose de la descendance : The Influence of Environment (Exercise and Diet) during Gestation on Oxidative Stress Status and Glucose Metabolism in Offspring.

Degree: Docteur es, Physiologie-Physiopathogies-Pharmacologie, 2016, Université Grenoble Alpes (ComUE); Universitas Indonesia

URL: http://www.theses.fr/2016GREAV049

► L'altération de la fonction mitochondriale, β-cellules pancréatiques, et l'homéostasie du glucose de la descendance de rat de l'exerciceContexte. Il est connu que l'environnement gestationnel est… (more)

▼ L'altération de la fonction mitochondriale, β-cellules pancréatiques, et l'homéostasie du glucose de la descendance de rat de l'exerciceContexte. Il est connu que l'environnement gestationnel est reconnue comme un facteur essentiel pour le développement du fœtus et étroitement liée à l'état de santé dans la vie plus tard. Environnement inadéquat pour le fœtus tels que le stress oxydatif intra-utérine est très nocif pour le développement du fœtus. Par conséquent, un effort de prévention de surmonter ce problème est nécessaire en modifiant l'environnement autour de la gestation par l'exercice durant cette période.Objectifs. Le but de cette étude était de connaître le rôle des mitochondries dans la prévention du stress oxydatif pendant la grossesse, en augmentant système antioxydant et la fonction du pancréas et dans l'amélioration de l'homéostasie du glucose de l'enfant de la mère qui a été induite par l'exercice régulier d'intensité modérée.Méthodes. Cette étude a été réalisée au Laboratoire de bioénergétique appliquée et fondamentale Inserm U1055, Université Joseph Fourier, Grenoble-France. Les sujets étaient rat Wistar femelle qui ont été divisés en 2 groupes, le groupe sédentaire et l'exercice. L'entraînement physique a été effectué 4 semaines avant et jusqu'à ce que le 18e jour de la gestation. Thiol et GPx ont été mesurées en utilisant un dosage biochimique. Consommation d'O2 a été mesurée avec oxygraphie. L'activité du complexe respiratoire, cytochrome et ROS ont été mesurées par spectrophotométrie. Citrate synthase, la protéine kinase B (PKB), quinone et tocophérol ont été mesurées en utilisant un dosage biochimique. La graisse viscérale et du pancréas total ont été pesés, et la taille de l'île de Langerhans a été analysé par la préparation de l'histologie. Les résultats ont été analysés en utilisant le test t.Résultats. Il a été constaté que l'état redox du nouveau-né est égal. La diminution de thiol suivie par une augmentation de l'activité antioxydante GPx (p <0,001). L'efficacité de la consommation et de l'activité du complexe respiratoire, suivie par une augmentation de cytochrome, la quinone et du tocopherol oxygène provoque la diminution de la production de ROS de la progéniture de rat de levées mère (p <0,001). La sécrétion d'insuline a augmenté de 74% et l'activité PKB était plus élevée (p <0,05). Tolérance au glucose et de l'insuline (âgés de 3 mois) étaient mieux (p <0,05), la taille de l'île de Langerhans et le poids du pancréas étaient plus petits (p = 0,021) chez la progéniture de rat de l'exercice mère. À l'âge de 3 mois, le taux de glucose dans le sang était inférieur de 8% (p = 0,046), l'insuline à jeun était plus élevé de 72% (p = 0,009) et le poids corporel était inférieur (p = 0,041) de la progéniture de rat de l'exercice mère par rapport à ceux de la mère sédentaire, mais le total de graisse viscérale était pas différente (p> 0,05).Conclusion. L'exercice régulier d'intensité modérée pour la mère de 4 semaines avant et jusqu'à ce que le 18e jour de la gestation a été très efficace pour… Advisors/Committee Members: Fontaine, Eric (thesis director).

Subjects/Keywords: Environnement; Gestation; GSH et GPX; Mitochondrie; Fetal programmation; Ros; Environment; GSH and GPX; Mitochondria; Fetal programming; Pregnancy; Ros; 610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Farida, S. (2016). Influence de l'environnement (exercice et nutrition) durant la gestation sur l’état de stress oxydant et le métabolisme du glucose de la descendance : The Influence of Environment (Exercise and Diet) during Gestation on Oxidative Stress Status and Glucose Metabolism in Offspring. (Doctoral Dissertation). Université Grenoble Alpes (ComUE); Universitas Indonesia. Retrieved from http://www.theses.fr/2016GREAV049

Chicago Manual of Style (16^th Edition):

Farida, Siti. “Influence de l'environnement (exercice et nutrition) durant la gestation sur l’état de stress oxydant et le métabolisme du glucose de la descendance : The Influence of Environment (Exercise and Diet) during Gestation on Oxidative Stress Status and Glucose Metabolism in Offspring.” 2016. Doctoral Dissertation, Université Grenoble Alpes (ComUE); Universitas Indonesia. Accessed January 18, 2021. http://www.theses.fr/2016GREAV049.

MLA Handbook (7^th Edition):

Farida, Siti. “Influence de l'environnement (exercice et nutrition) durant la gestation sur l’état de stress oxydant et le métabolisme du glucose de la descendance : The Influence of Environment (Exercise and Diet) during Gestation on Oxidative Stress Status and Glucose Metabolism in Offspring.” 2016. Web. 18 Jan 2021.

Vancouver:

Farida S. Influence de l'environnement (exercice et nutrition) durant la gestation sur l’état de stress oxydant et le métabolisme du glucose de la descendance : The Influence of Environment (Exercise and Diet) during Gestation on Oxidative Stress Status and Glucose Metabolism in Offspring. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); Universitas Indonesia; 2016. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2016GREAV049.

Council of Science Editors:

Farida S. Influence de l'environnement (exercice et nutrition) durant la gestation sur l’état de stress oxydant et le métabolisme du glucose de la descendance : The Influence of Environment (Exercise and Diet) during Gestation on Oxidative Stress Status and Glucose Metabolism in Offspring. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); Universitas Indonesia; 2016. Available from: http://www.theses.fr/2016GREAV049

12. Σταματίου, Ειρήνη. Μέθοδος προσδιορισμού της γενικής και ειδικής θειολικής οξειδοαναγωγικής κατάστασης των οργανισμών.

Degree: 2010, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3794

►

Η ολοκληρωμένη εκτίμηση της θειολικής οξειδοαναγωγικής κατάστασης (ΘΟΚ) ενός οργανισμού ιστού ή κυττάρου είναι πολύ σημαντική καθώς οξειδοαναγωγικές αλλαγές των διαφόρων θειολικών μορίων συνδέονται με… (more)

▼

Η ολοκληρωμένη εκτίμηση της θειολικής οξειδοαναγωγικής κατάστασης (ΘΟΚ) ενός οργανισμού ιστού ή κυττάρου είναι πολύ σημαντική καθώς οξειδοαναγωγικές αλλαγές των διαφόρων θειολικών μορίων συνδέονται με το οξειδωτικό στρες και με αρκετές ασθένειες. Η γενική ΘΟΚ (ΓΘΟΚ) χαρακτηρίζεται από τις συγκεντρώσεις ορισμένων συνόλων θειολικών μορίων στην αναγμένη και την οξειδωμένη μορφή τους (θειολικά οξειδοαναγωγικά ζεύγη). Αυτά τα ζεύγη μπορεί να είναι μη πρωτεϊνικά (non-protein ή NP) (όπως NPSH και NPSSNP με το NP να συμβολίζει οποιαδήποτε άλλη μη πρωτεϊνική θειόλη) ή πρωτεϊνικά (protein ή P) (όπως PSH, PSSP και PSSNP). Ειδικότερα, οι κυριότερες μη πρωτεϊνικές θειόλες γλουταθειόνη (GSH) και κυστεΐνη (CSH) μαζί με τα συμμετρικά, μεικτά δισουλφίδιά τους και τις οξειδωμένες τους μορφές (GSSG, PSSG, PSSC, NPGSHox, NPCSHox,) είναι τα οξειδοαναγωγικά ζεύγη τα οποία χαρακτηρίζουν την ειδική ΘΟΚ (ΕΘΟΚ), καθώς είναι εκείνα που απαντώνται σε υψηλότερη συγκέντρωση στους οργανισμούς. Στη διεθνή βιβλιογραφία δεν υπάρχει μεθοδολογία για την ταυτόχρονη ποσοτικοποίηση των θειολικών μορίων που χαρακτηρίζουν τη ΘΟΚ των οργανισμών. Συνεπώς, στόχος της παρούσας μελέτης είναι η ανάπτυξη μιας νέας μεθόδου ποσοτικοποίησης τόσο της ΓΘΟΚ όσο και της ΕΘΟΚ, που να είναι εφαρμόσιμη σε όλους τους οργανισμούς. Για το διαχωρισμό πρωτεϊνικών και μη πρωτεϊνικών μορίων χρησιμοποιήθηκε το τριχλωροακετικό οξύ που σε ορισμένη συγκέντρωση (>5%) καταβυθίζει αποτελεσματικά όλες τις πρωτεΐνες. Ο ποσοτικός προσδιορισμός των δισουλφιδικών μορίων και οξειδωμένων μορφών (NPSSNP, PSSP, PSSNP, GSSG, NPGSHox, NPCSHox, PSSG και PSSC) πραγματοποιήθηκε μετά από αναγωγή τους (με το αντιδραστήριο tributyl phosphine), ενώ ο ποσοτικός προσδιορισμός των ελεύθερων θειολών (PSH, NPSH, GSH και CSH) πραγματοποιήθηκε χωρίς την αναγωγή τους. Ειδικότερα, η ποσοτικοποίηση των αναγμένων διθειολικών ομάδων (δισουλφιδίων) και των ελεύθερων θειολών έγιναν με τα αντιδραστήρια 4,4-dithiodipyridine (για τις -SH ομάδες των αναγμένων δισουλφιδίων, καθώς και για τις ελεύθερες NPSH και PSH), o-phthalaldehyde (για την GSH, GSSG και NPGSHox) και νινυδρίνη (για την CSH και την NPCSHox), σε συνδυασμό με κατάλληλη μαθηματική επεξεργασία βασισμένη στη στοιχειομετρία των αντιδράσεων αναγωγής. Η υψηλή ευαισθησία της μεθόδου (στο επίπεδο του nmol) την καθιστά εφαρμόσιμη ακόμη και σε βιολογικά δείγματα χαμηλής περιεκτικότητας σε θειόλες (όπως πχ. το οφθαλμικό και το εγκεφαλονωτιαίο υγρό).

The thiol redox state (TRS) is an essential condition of prokaryotic and eukaryotic cells associated with all major biological processes. The general TRS (GTRS) part of it, is characterized by the levels of all thiol compounds of protein or non-protein origin in their reduced or oxidized form (thiol redox couples), while the specific TRS (STRS) by the levels of certain thiols, reduced and oxidized, free or membrane bound. The GTRS redox couples are composed of non-protein (NP) (such as NPSH and NPSSNP) or protein (P) (such as PSH, PSSP and PSSNP) thiols. On the other hand, the STRS redox couples are composed of the main…

Advisors/Committee Members: Γεωργίου, Χρήστος, Stamatiou, Irene, Παναγόπουλος, Νικόλαος, Κεφαλιακού, Μαρίνα, Γεωργίου, Χρήστος.

Subjects/Keywords: Θειολική οξειδοαναγωγική κατάσταση; Γλουταθειόνη; Thiol redox state; Glutathione (GSH)

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Σταματίου, �. (2010). Μέθοδος προσδιορισμού της γενικής και ειδικής θειολικής οξειδοαναγωγικής κατάστασης των οργανισμών. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3794

Chicago Manual of Style (16^th Edition):

Σταματίου, Ειρήνη. “Μέθοδος προσδιορισμού της γενικής και ειδικής θειολικής οξειδοαναγωγικής κατάστασης των οργανισμών.” 2010. Masters Thesis, University of Patras. Accessed January 18, 2021. http://nemertes.lis.upatras.gr/jspui/handle/10889/3794.

MLA Handbook (7^th Edition):

Σταματίου, Ειρήνη. “Μέθοδος προσδιορισμού της γενικής και ειδικής θειολικής οξειδοαναγωγικής κατάστασης των οργανισμών.” 2010. Web. 18 Jan 2021.

Vancouver:

Σταματίου �. Μέθοδος προσδιορισμού της γενικής και ειδικής θειολικής οξειδοαναγωγικής κατάστασης των οργανισμών. [Internet] [Masters thesis]. University of Patras; 2010. [cited 2021 Jan 18]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3794.

Council of Science Editors:

Σταματίου �. Μέθοδος προσδιορισμού της γενικής και ειδικής θειολικής οξειδοαναγωγικής κατάστασης των οργανισμών. [Masters Thesis]. University of Patras; 2010. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3794

Penn State University

13. Maddineni, Sreenivasa. EFFECTS OF METHIONINE RESTRICTION ON GLUTATHIONE RELATED PATHWAYS.

Degree: 2010, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/10777

► The tripeptide glutathione (GSH) is the principal antioxidant in cells and its depletion is associated with the onset of age-related chronic diseases including cardiovascular disease,… (more)

Subjects/Keywords: Methionine restriction; GSH; GPX; GR

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maddineni, S. (2010). EFFECTS OF METHIONINE RESTRICTION ON GLUTATHIONE RELATED PATHWAYS. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/10777

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maddineni, Sreenivasa. “EFFECTS OF METHIONINE RESTRICTION ON GLUTATHIONE RELATED PATHWAYS.” 2010. Thesis, Penn State University. Accessed January 18, 2021. https://submit-etda.libraries.psu.edu/catalog/10777.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maddineni, Sreenivasa. “EFFECTS OF METHIONINE RESTRICTION ON GLUTATHIONE RELATED PATHWAYS.” 2010. Web. 18 Jan 2021.

Vancouver:

Maddineni S. EFFECTS OF METHIONINE RESTRICTION ON GLUTATHIONE RELATED PATHWAYS. [Internet] [Thesis]. Penn State University; 2010. [cited 2021 Jan 18]. Available from: https://submit-etda.libraries.psu.edu/catalog/10777.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maddineni S. EFFECTS OF METHIONINE RESTRICTION ON GLUTATHIONE RELATED PATHWAYS. [Thesis]. Penn State University; 2010. Available from: https://submit-etda.libraries.psu.edu/catalog/10777

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

14. Yang, Ya-Po. Study of optical properties of Au/Glutathione nanoparticles solution using Terahertz time domain spectroscopy.

Degree: Master, Physics, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811117-145844

► In this thesis we mainly do research about optical properties of luminous solution that has gold nanoparticles in Terahertz spectral region using terahertz time-domain spectroscopy… (more)

Subjects/Keywords: dielectric constant; refractive index; gold nanoparticles; THz-TDS; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yang, Y. (2017). Study of optical properties of Au/Glutathione nanoparticles solution using Terahertz time domain spectroscopy. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811117-145844

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Ya-Po. “Study of optical properties of Au/Glutathione nanoparticles solution using Terahertz time domain spectroscopy.” 2017. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811117-145844.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Ya-Po. “Study of optical properties of Au/Glutathione nanoparticles solution using Terahertz time domain spectroscopy.” 2017. Web. 18 Jan 2021.

Vancouver:

Yang Y. Study of optical properties of Au/Glutathione nanoparticles solution using Terahertz time domain spectroscopy. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811117-145844.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang Y. Study of optical properties of Au/Glutathione nanoparticles solution using Terahertz time domain spectroscopy. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811117-145844

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

15. Hajdu, Márton. Az Aspergillus nidulans glutation lebontása .

Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, University of Debrecen

URL: http://hdl.handle.net/2437/213025

► Vizsgálataink célja azon hipotézis tesztelése, miszerint az Aspergillus nidulans fonalas gombában a DUG útvonal felelős a GSH intracelluláris lebontásáért. A Saccharomyces cerevisiae dug1-3 génjeinek ortológjai… (more)

Subjects/Keywords: Aspergillus; nidulans; glutation; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hajdu, M. (n.d.). Az Aspergillus nidulans glutation lebontása . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/213025

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hajdu, Márton. “Az Aspergillus nidulans glutation lebontása .” Thesis, University of Debrecen. Accessed January 18, 2021. http://hdl.handle.net/2437/213025.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hajdu, Márton. “Az Aspergillus nidulans glutation lebontása .” Web. 18 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Hajdu M. Az Aspergillus nidulans glutation lebontása . [Internet] [Thesis]. University of Debrecen; [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2437/213025.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Hajdu M. Az Aspergillus nidulans glutation lebontása . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/213025

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Universidad de Chile

16. Flores Martínez, Romina Valeska. Creciendo en un ambiente ruidoso: efectos del ruido urbano sobre aspectos fisiológicos y el aprendizaje en un ave de desarrollo precoz.

Degree: 2018, Universidad de Chile

URL: http://repositorio.uchile.cl/handle/2250/174286

► Entender de qué manera afecta el sonido generado por las grandes ciudades a las poblaciones subyacentes de animales es uno de los ítems de interés… (more)

▼ Entender de qué manera afecta el sonido generado por las grandes ciudades a las poblaciones subyacentes de animales es uno de los ítems de interés en la ecología de ambientes urbanos. Sin embargo, los impactos del ruido antropogénico sobre las aves son aun poco conocidos. Por ello, el objetivo principal del presente trabajo fue determinar si el ruido de ciudad percibido durante el desarrollo de un individuo representa un factor de estrés, inferido a través de los niveles de glutatión, y si dicho estrés afecta el comportamiento de aprendizaje y memoria. Para ello se realizó un estudio experimental en el cual un grupo de codornices (Coturnix japonica) fue sometido, durante una semana a ruido de ciudad, mientras otro grupo se mantuvo durante el mismo período de tiempo sometida a ruido rural. Posterior a este periodo, se tomaron muestras de sangre para analizar los niveles plasmáticos de uno de los principales antioxidantes y marcador de estrés ambiental, el glutatión (GSH) para luego evaluar la capacidad de memoria y aprendizaje. Para esto, un subgrupo de las codornices fue separado y sometido a un proceso de acondicionamiento, mediante la utilización de un laberinto con recompensa. Se midió el tiempo que cada codorniz demoró en encontrar la recompensa, y el número de veces que falló. Encontramos que los niveles de GSH fueron significativamente mayores en el grupo con el ruido de ciudad, y además los individuos que permanecieron más tiempo en la posición estática (inmovilidad tónica) fueron a la vez, los que presentaron los mayores niveles de este antioxidante. En cuanto al aprendizaje, en el tercer día de acondicionamiento las aves del grupo con ruido urbano, demoraron significativamente más tiempo en encontrar la recompensa. Se sugiere que el ruido de ciudad representa una importante variable de estrés para las aves, generando una mayor movilización del sistema antioxidante GSH y disminuyendo las capacidades cognitivas y de aprendizaje en aves precociales.; Understanding how the sound generated by large cities affects the underlying populations of animals is one of the items of interest in the ecology of urban environments. However, the impacts of anthropogenic noise on birds are still poorly understood. Therefore, the main objective of the present work was to determine if the city noise perceived during the development of an individual represents a stress factor, inferred through glutathione levels, and if said stress affects the learning and memory behavior. To this end, this experimental study was carried out in which a group of quail (Coturnix japonica) was subjected, for a week, to city noise, while another group was subjected to rural noise during the same period of time. Later in this period, blood samples were taken to analyze the plasma levels of one of the main antioxidants and marker of environmental stress, glutathione (GSH), to later evaluate the memory and learning capacity. For this, a subgroup of quail was separated and subjected to a conditioning process, using a…

Subjects/Keywords: Ruido urbano; Aves; capacidad de aprendizaje; Memoria; Glutation (GSH); Coturnix japonica

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Flores Martínez, R. V. (2018). Creciendo en un ambiente ruidoso: efectos del ruido urbano sobre aspectos fisiológicos y el aprendizaje en un ave de desarrollo precoz. (Thesis). Universidad de Chile. Retrieved from http://repositorio.uchile.cl/handle/2250/174286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Flores Martínez, Romina Valeska. “Creciendo en un ambiente ruidoso: efectos del ruido urbano sobre aspectos fisiológicos y el aprendizaje en un ave de desarrollo precoz.” 2018. Thesis, Universidad de Chile. Accessed January 18, 2021. http://repositorio.uchile.cl/handle/2250/174286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Flores Martínez, Romina Valeska. “Creciendo en un ambiente ruidoso: efectos del ruido urbano sobre aspectos fisiológicos y el aprendizaje en un ave de desarrollo precoz.” 2018. Web. 18 Jan 2021.

Vancouver:

Flores Martínez RV. Creciendo en un ambiente ruidoso: efectos del ruido urbano sobre aspectos fisiológicos y el aprendizaje en un ave de desarrollo precoz. [Internet] [Thesis]. Universidad de Chile; 2018. [cited 2021 Jan 18]. Available from: http://repositorio.uchile.cl/handle/2250/174286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Flores Martínez RV. Creciendo en un ambiente ruidoso: efectos del ruido urbano sobre aspectos fisiológicos y el aprendizaje en un ave de desarrollo precoz. [Thesis]. Universidad de Chile; 2018. Available from: http://repositorio.uchile.cl/handle/2250/174286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Carolina

17. XUE, ZUQIN. Using GFP-Based Redox and pH Sensors to Monitor Glutathione Metabolism In the Mitochondrial Intermembrane Space.

Degree: MS, Chemistry and Biochemistry, 2011, University of South Carolina

URL: https://scholarcommons.sc.edu/etd/747

► Redox homeostasis is essential for all living organisms. Maintaining thiol-disulfide balance is especially important for the mitochondrion, since it is the main source and… (more)

▼ Redox homeostasis is essential for all living organisms. Maintaining thiol-disulfide balance is especially important for the mitochondrion, since it is the main source and target of redox oxygen species (ROS) that can alter the balance. To gauge the redox states of the mitochondrial matrix and intermembrane space, we targeted green fluorescent protein (GFP) based redox and pH sensors to these specific compartments in the model organism Saccharomyces cerevisiae. The correct localization was verified by subcellular fractionation and western blot. These genetically engineered sensors allow us to determine the in vivo pH and redox change of distinct subcellular compartments. By using these sensors, we studied the pH change upon adding glutathione (GSH) to HGT1 (High-affinity glutathione transporter 1) transformed yeast cells. We successfully determined the pH change with high accuracy and sensitivity upon adding GSH/GSSG to the media. The results demonstrate that HGT1 co-transports GSH and H+. To test the effect of endogenous GSH:GSSG upon the change of pH, we also used a glr1Ä strain (GLR1 is a gene encoding both cytosolic and mitochondrial forms of glutathione reductase) and found no significant difference in pH between the wild type cells and glr1Ä cells. Glutathione and mitochondrion also play a significant role in the maintenance of iron homeostasis in yeast. Since the mitochondrion is the sole site of heme synthesis and the majority of iron sulfur cluster (ISC) generation, it is a focal point of iron metabolism. Previous research has indicated that the mitochondrial ISC assembly machinery is required for the maturation of both mitochondrial and cytosolic Fe-S proteins. The IMS-localized sulfhydryl oxidase Erv1 and GSH are both proposed to be involved in cytosolic iron-sulfur protein biogenesis. To reveal the mechanism of this connection, we measured the total GSH in several temperature-sensitive erv1/mia40 mutants. Mia40 is an import receptor that functions with Erv1 to oxidize cysteines in the IMS. Our results show that cytosolic GSH depletion is found only in one particular erv1 mutant and none of the mia40 mutants. Thus, the Mia40-Erv1 IMS import path way may not be directly involved in cytosolic Fe-S protein maturation as proposed before. Instead, specific mutations in erv1 may influence GSH levels, which in turn cause disruption of cytosolic Fe-S cluster maturation. Advisors/Committee Members: Caryn E Outten.

Subjects/Keywords: Erv1; GFP; GSH; Iron-sulfur cluster; pH sensor; redox sensor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

XUE, Z. (2011). Using GFP-Based Redox and pH Sensors to Monitor Glutathione Metabolism In the Mitochondrial Intermembrane Space. (Masters Thesis). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/747

Chicago Manual of Style (16^th Edition):

XUE, ZUQIN. “Using GFP-Based Redox and pH Sensors to Monitor Glutathione Metabolism In the Mitochondrial Intermembrane Space.” 2011. Masters Thesis, University of South Carolina. Accessed January 18, 2021. https://scholarcommons.sc.edu/etd/747.

MLA Handbook (7^th Edition):

XUE, ZUQIN. “Using GFP-Based Redox and pH Sensors to Monitor Glutathione Metabolism In the Mitochondrial Intermembrane Space.” 2011. Web. 18 Jan 2021.

Vancouver:

XUE Z. Using GFP-Based Redox and pH Sensors to Monitor Glutathione Metabolism In the Mitochondrial Intermembrane Space. [Internet] [Masters thesis]. University of South Carolina; 2011. [cited 2021 Jan 18]. Available from: https://scholarcommons.sc.edu/etd/747.

Council of Science Editors:

XUE Z. Using GFP-Based Redox and pH Sensors to Monitor Glutathione Metabolism In the Mitochondrial Intermembrane Space. [Masters Thesis]. University of South Carolina; 2011. Available from: https://scholarcommons.sc.edu/etd/747

University of Louisville

18. Rogers, Erica Nicole. The role of curcumin in response to BPDE-induced DNA damage in human lung epithelial cells.

Degree: PhD, 2011, University of Louisville

URL: 10.18297/etd/1226 ; https://ir.library.louisville.edu/etd/1226

► Long-term exposure to harmful carcinogens like benzo[a]pyrene (BaP) are linked to lung cancer. Benzo[a]pyrene diol epoxide (BPDE), the ultimate carcinogen produced by BaP bioactivation, is… (more)

▼ Long-term exposure to harmful carcinogens like benzo[a]pyrene (BaP) are linked to lung cancer. Benzo[a]pyrene diol epoxide (BPDE), the ultimate carcinogen produced by BaP bioactivation, is believed to play an important role in lung carcinogenesis. The tumor suppressor protein is p53 signals cell cycle arrest, DNA repair, and apoptosis in response to BPDE-induced damage. However, physiological exposure to BPDE fails to activate p53. Although the bioactive principle of turmeric spice, curcumin, shows promising effects against BPDE-induced carcinogenesis, its exact mode of action remains unclear. Therefore the aim of this dissertation was to investigate whether curcumin prevents BPDE-induced DNA damage by regulating p53-mediated cellular responses in A549/LXSN (p53+) and A549/E6 (p53-) cells. I hypothesize that curcumin may reduce BPDE-induced DNA damage by lowering the threshold of p53 activation, thereby inducing p53-mediated mechanisms. Curcumin pretreatment reduced BPDEDNA adducts in a p53-dependent manner. However, p53-regulated proteins XPC and DDB2 did not change with curcumin pretreatment and p53-expression. Curcumin pretreatment did not change the overall repair rate in p53+ cells. Curcumin pretreatment, p53-expression, and BPDE exposure did not significantly change glutathione-S-transferase (GST) levels or activity as well as glutathione (GSH) levels. Curcumin increased levels of p53, phosphorylated p53 at Ser15 (P-p53S15), and CDKN1A, but decreased phosphorylated retinoblastoma at Ser807/811 (P-pRbS807/811) in p53+ cells with lower BPDE exposure. Curcumin and BPDE increased and decreased PpRbS807/ 811, respectively, in p53- cells. BPDE induced S phase arrest in both cell lines. P53+ curcumin pretreated cells escaped S phase arrest earlier with 300 nM BPDE in contrast to BPDE treatment alone. Yet, curcumin did not change S phase arrest in p53+ cells treated with 50 or 100 nM BPDE. P53- cells did not escape S phase arrest 24 h after low (50 and 100 nM) BPDE exposures; p53- curcumin pretreated cells entered S phase arrest earlier. Phosphorylated CHK1 Ser345 (pCHKS345) levels, an indicator of S phase arrest, was higher in p53- cells than p53+ cells; curcumin increased pCHK1 Ser345. Curcumin also induced apoptosis earlier in p53- cells exposed to 300nM BPDE. Therefore, this dissertation shows that curcumin prevents BPDE-induced DNA damage by activating a host of mechanisms independent of p53 expression. Advisors/Committee Members: States, J. Christopher.

Subjects/Keywords: Curcumin; Cell cycle progression; P53; BPDE; DNA repair; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rogers, E. N. (2011). The role of curcumin in response to BPDE-induced DNA damage in human lung epithelial cells. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/1226 ; https://ir.library.louisville.edu/etd/1226

Chicago Manual of Style (16^th Edition):

Rogers, Erica Nicole. “The role of curcumin in response to BPDE-induced DNA damage in human lung epithelial cells.” 2011. Doctoral Dissertation, University of Louisville. Accessed January 18, 2021. 10.18297/etd/1226 ; https://ir.library.louisville.edu/etd/1226.

MLA Handbook (7^th Edition):

Rogers, Erica Nicole. “The role of curcumin in response to BPDE-induced DNA damage in human lung epithelial cells.” 2011. Web. 18 Jan 2021.

Vancouver:

Rogers EN. The role of curcumin in response to BPDE-induced DNA damage in human lung epithelial cells. [Internet] [Doctoral dissertation]. University of Louisville; 2011. [cited 2021 Jan 18]. Available from: 10.18297/etd/1226 ; https://ir.library.louisville.edu/etd/1226.

Council of Science Editors:

Rogers EN. The role of curcumin in response to BPDE-induced DNA damage in human lung epithelial cells. [Doctoral Dissertation]. University of Louisville; 2011. Available from: 10.18297/etd/1226 ; https://ir.library.louisville.edu/etd/1226

19. Melis, Nicolas. Identification de deux nouvelles cibles dans la gestion du stress oxydatif ; la protéine CFTR et la voie d’activation d’eIF5A : Management of oxidative stress, involvement of two news targets : CFTR and activation pathway of eIF5A.

Degree: Docteur es, Sciences de la vie, 2015, Nice

URL: http://www.theses.fr/2015NICE4030

►

Le stress oxydatif définit un phénomène cellulaire particulier caractérisé par un niveau élevé de molécules hautement réactives, essentiellement lié à l’utilisation de l’oxygène par les… (more)

▼

Le stress oxydatif définit un phénomène cellulaire particulier caractérisé par un niveau élevé de molécules hautement réactives, essentiellement lié à l’utilisation de l’oxygène par les systèmes biologiques via la respiration. La dérégulation de l’état oxydatif de la cellule est à l’origine soit de processus d’adaptations efficaces (adaptation à l’altitude) soit de pathologies (AVC, infarctus). Ce travail de thèse s’est porté sur l’étude de deux nouvelles cibles pouvant induire une résistance/tolérance à la variation du stress oxydatif : la première est la protéine canal CFTR («Cystic Fibrosis Transmembrane conductance Regulator») et la seconde la voie d’activation d’eIF5A («eukaryotic Initiation translation Factor 5A»). Nous avons pu mettre en évidence que la protéine CFTR grâce à sa perméabilité au glutathion (l’antioxydant majoritaire cellulaire) est un modulateur de l’état oxydatif de la cellule, que ce soit lors de l’exposition à des agents cytotoxiques (cisplatine) ou lors de l’adaptation à des conditions hypoxiques chroniques. La deuxième cible identifiée est le facteur eIF5A qui est la seule protéine activée par la fixation d’un résidu hypusine. L’inhibition de cette modification post-traductionnelle protège les cellules d’une production d’espèces réactives induite par l’anoxie. Cette résistance à l’anoxie est accompagnée d’un profond remodelage métabolique et mitochondrial. Sur des modèles animaux d’ischémie (rein et cerveau), l’inhibition de l’activation d’eIF5A conduit à une protection des organes face à un manque d’oxygène. Ces études fondamentales ont des applications cliniques potentielles dans des pathologies humaines (infarctus, AVC, transplantation).

Oxidative stress represents a particular cellular condition, characterized by an intracellular increase in thereactive species level. These species are highly reactive towards biomolecules and result of oxygenconsumption by biological systems essentially through respiration. Deregulation of the cellular oxidativestate can initiate adaptive processes (as elevation adaptation) or several human pathologies (stroke,infarct). This thesis work has been devoted to the study of two news potential targets allowing atolerance/resistance towards disequilibrium of oxidative stress; the first one is CFTR, a channel protein(«Cystic Fibrosis Transmembrane conductance Regulator»), and the second one is the activation pathwayof the translation factor eIF5A («eukaryotic Initiation translation Factor 5A»). Based on the peculiaractivity of CFTR, consisting in the transport of glutathione, the major antioxidant of the cell, weevidenced the role of CFTR in the management of cellular oxidative state during cytotoxic drugexposure (cisplatin) or during adaptation to chronical hypoxia. The second target, eIF5A is the only oneprotein described as post-translationally modified by fixation of a hypusine residue. We demonstratedthat inhibition of eiF5A activation protect cells from reactive oxygen species generated during anoxia. Atcellular level, this protection is accompanied…

Advisors/Committee Members: Duranton, Christophe (thesis director), Rubera, Isabelle (thesis director).

Subjects/Keywords: EIf5A; CFTR; Stress oxydatif; Tolérance; Hypoxie; Anoxie; Cisplatine; Inhibiteurs; DHPS; DOHH; GSH; EIf5A; CFTR; Oxidative stress; Tolerance; Hypoxia; Anoxia; Cisplatin; Inhibitors; DHPS; DOHH; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melis, N. (2015). Identification de deux nouvelles cibles dans la gestion du stress oxydatif ; la protéine CFTR et la voie d’activation d’eIF5A : Management of oxidative stress, involvement of two news targets : CFTR and activation pathway of eIF5A. (Doctoral Dissertation). Nice. Retrieved from http://www.theses.fr/2015NICE4030

Chicago Manual of Style (16^th Edition):

Melis, Nicolas. “Identification de deux nouvelles cibles dans la gestion du stress oxydatif ; la protéine CFTR et la voie d’activation d’eIF5A : Management of oxidative stress, involvement of two news targets : CFTR and activation pathway of eIF5A.” 2015. Doctoral Dissertation, Nice. Accessed January 18, 2021. http://www.theses.fr/2015NICE4030.

MLA Handbook (7^th Edition):

Melis, Nicolas. “Identification de deux nouvelles cibles dans la gestion du stress oxydatif ; la protéine CFTR et la voie d’activation d’eIF5A : Management of oxidative stress, involvement of two news targets : CFTR and activation pathway of eIF5A.” 2015. Web. 18 Jan 2021.

Vancouver:

Melis N. Identification de deux nouvelles cibles dans la gestion du stress oxydatif ; la protéine CFTR et la voie d’activation d’eIF5A : Management of oxidative stress, involvement of two news targets : CFTR and activation pathway of eIF5A. [Internet] [Doctoral dissertation]. Nice; 2015. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2015NICE4030.

Council of Science Editors:

Melis N. Identification de deux nouvelles cibles dans la gestion du stress oxydatif ; la protéine CFTR et la voie d’activation d’eIF5A : Management of oxidative stress, involvement of two news targets : CFTR and activation pathway of eIF5A. [Doctoral Dissertation]. Nice; 2015. Available from: http://www.theses.fr/2015NICE4030

Université Paris-Sud – Paris XI

20. Igbaria, Aeid. Functional redox compartmentation of GSH in the yeast Saccharomyces cerevisiae : Compartimentalisation du glutathion dans les cellules de levure S. cerevisiae et de ses conséquences fonctionnelles.

Degree: Docteur es, Biologie, 2011, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2011PA112189

► L'oxydation des résidus cystéines est une modification biochimique très répandue survenant dans tous les compartiments des cellules eucaryotes. Ce phénomène sert le repliement oxydatif des… (more)

▼ L'oxydation des résidus cystéines est une modification biochimique très répandue survenant dans tous les compartiments des cellules eucaryotes. Ce phénomène sert le repliement oxydatif des protéines dans le réticulum endoplasmique (RE), l'importation de protéines dans l'espace intermembranaire de la mitochondrie (IMS). De plus, il a un rôle régulateur dans la matrice mitochondriale et dans le cytosol où il contrôle l’activité des enzymes et des protéines de signalisation et de régulation. Dans tous ces procédés, la réversibilité de l'oxydation des résidus Cys est une caractéristique essentielle. Deux systèmes oxydoréductase puissants existent : les voies de glutathion (GSH) et la thiorédoxine ; ils catalysent la réduction des ponts disulfure, et contrôlent la plupart des processus cellulaires thiol-redox dépendant. Cependant, en dépit d'énormes connaissances portant sur leur enzymologie, peu est connu sur les caractéristiques physiologiques de ces systèmes chez les eucaryotes. Pour déterminer l'importance physiologique de ces systèmes et indiquer lequel est à la base de l'exigence du GSH pour la viabilité, nous avons effectué une analyse complète des cellules de levure épuisée ou contenant des niveaux toxiques de GSH. Les deux conditions déclenchent une réponse « iron-starvation-like » et une altération de l'activité des enzymes d’assemblage des centres fer-soufre (Iron sulfure cluster : ISC) extra-mitochondriales. Cependant, elles n’ont pas d'impact sur l’entretien thiol redox, à l’exception des niveaux élevés de glutathion qui ont altéré le repliement oxydatif des protéines dans le reticulum endoplasmique. Alors que le fer sauve partiellement la maturation des ISC et les défauts de croissance des cellules appauvries eh GSH, des expériences génétiques ont indiqué que, contrairement à la thiorédoxine, le glutathion ne peut pas assurer par lui-même les fonctions thiol-redox de la cellule. Nous proposons que le glutathion soit essentiel par son exigence dans l’assemblage des centres fer-soufre, mais ne serve comme backup que pour maintenir l’état thiol-redox de la cellule. Des niveaux physiologiques élevés de GSH sont ainsi destinés à isoler sa fonction dans le métabolisme du fer des variations de sa concentration pendant le stress redox, ce qui constitue un modèle contestant la vision traditionnelle du GSH comme acteur primordial du contrôle thiol-redox cytosolique.Nos données préliminaires sur la distribution de GSH dans les cellules recueillies par lasurveillance de l'état redox de rxYFP ciblée pour différents compartiments cellulaires (RE,Matrice, cytosol et IMS) dans les cellules HGT1 indiquent un transport spécifique du GSH vers le RE et l'exportation de GSSG de ce compartiment. Nous avons pu caractériser deuxtransporteurs ABC dont la suppression modifie le RE plus oxydant et entraîne une accumulation de GSSG par rapport aux cellules sauvages. Ces données ont été confirmées par le suivi de l'état redox de PDI1 et ERO1 (WT et hyper active). Elles suggèrent un rôle de ces transporteurs dans l'exportation du GSSG du… Advisors/Committee Members: Toledano, Michel (thesis director).

Subjects/Keywords: GSH; Thiorédoxine; Centres fer-soufre; UPR; Repliement oxydatif des protéines; Thiol-redox; Cystéines; GSH; Thioredoxin; Iron sulfur cluster; UPR; Oxidative Protein folding; Thiol-redox; Cysteine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Igbaria, A. (2011). Functional redox compartmentation of GSH in the yeast Saccharomyces cerevisiae : Compartimentalisation du glutathion dans les cellules de levure S. cerevisiae et de ses conséquences fonctionnelles. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2011PA112189

Chicago Manual of Style (16^th Edition):

Igbaria, Aeid. “Functional redox compartmentation of GSH in the yeast Saccharomyces cerevisiae : Compartimentalisation du glutathion dans les cellules de levure S. cerevisiae et de ses conséquences fonctionnelles.” 2011. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 18, 2021. http://www.theses.fr/2011PA112189.

MLA Handbook (7^th Edition):

Igbaria, Aeid. “Functional redox compartmentation of GSH in the yeast Saccharomyces cerevisiae : Compartimentalisation du glutathion dans les cellules de levure S. cerevisiae et de ses conséquences fonctionnelles.” 2011. Web. 18 Jan 2021.

Vancouver:

Igbaria A. Functional redox compartmentation of GSH in the yeast Saccharomyces cerevisiae : Compartimentalisation du glutathion dans les cellules de levure S. cerevisiae et de ses conséquences fonctionnelles. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2011. [cited 2021 Jan 18]. Available from: http://www.theses.fr/2011PA112189.

Council of Science Editors:

Igbaria A. Functional redox compartmentation of GSH in the yeast Saccharomyces cerevisiae : Compartimentalisation du glutathion dans les cellules de levure S. cerevisiae et de ses conséquences fonctionnelles. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2011. Available from: http://www.theses.fr/2011PA112189

NSYSU

21. Chen, Tzu-Heng. Novel Synthesis and Applications of Gold Nanoclusters : (1) Sensing of GSH, (2) Sensing of Mercury(II), and (3) Cluster-Assisted LDI MS.

Degree: Master, Chemistry, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711112-110624

► This paper, contain three parts, focus on develop synthesis method for novel functional nanocluster, base on its special optical and reactive property. In the first… (more)

▼ This paper, contain three parts, focus on develop synthesis method for novel functional nanocluster, base on its special optical and reactive property. In the first part, lysozyme VI was mixed with HAuCl4 under acidic condition(pH~3) and yield blue fluorescent Au8 nanocluster. When this Au8 cluster was incubate under alkaline condition (pH~12) a size evolution from Au8 to Au25 took place and monitored with fluorescence spectrum, MALDI MS, DLS. On the other hand, on the role of structural characteristic, fluorescence of Au8 cluster can be quenched by GSH as GSH induced core-etching reaction took place. Take advantage of this reaction, GSH in RBC can be quantitatively analysis by Au8 cluster. Compare with standard medical analysis method, this assay got comparable quantitative result and advantage in environmental friendly, low cost and low sample demand. Follow the first part, it was knowing that Hg2+quench fluorescence of Au25 but Au8 cluster. The second part of research development a ratiometric fluorescence assay for Hg2+ in drinking water sample, by control size evolution of nanocluster mentioned in first part by adding Ag+ into protein direct synthesis nanocluter process. Under optimize condition of bimetallic nanocluster synthesis, two separately fluorescence peak locate under 613 nm and 471 nm represent bigger size and smaller nanoclusters. Using fluorescence intensity ratio of two wavelength, concentration of Hg2+ in water sample can be determined by standard addition method with high reproducibility. Limit of detection was determined to be 0.1 and 0.4 nM, both lower than EPA permitted level in drinking water. On the third part, SA and HAuCl4 was mixed under water contained acetonitrile solution and SA caped nanocluster was form. This nanocluster after self essemble possess special crystalline and surface phenomenon and fit matrix for high reproducible laser desorption/ionization mass spectrometry. Compare with traditional matrix SA, the cluster matrix show 6.6% in RSD with 33%. And the desorption/ionization efficiency of nanocluster matrix is also much higher. Insulin, myoglobin and HSA sample can be quantitative with this matrix with mean RSD lower than 10%. Advisors/Committee Members: Shiuh-Jen Jiang (chair), Wei-Lung Tseng (committee member), Kwang-Ming Lee (chair).

Subjects/Keywords: fluorescence; matrix assisted laser desorption/ionization mass spectrometry; Hg2+; gold nanocluster; GSH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, T. (2012). Novel Synthesis and Applications of Gold Nanoclusters : (1) Sensing of GSH, (2) Sensing of Mercury(II), and (3) Cluster-Assisted LDI MS. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711112-110624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Tzu-Heng. “Novel Synthesis and Applications of Gold Nanoclusters : (1) Sensing of GSH, (2) Sensing of Mercury(II), and (3) Cluster-Assisted LDI MS.” 2012. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711112-110624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Tzu-Heng. “Novel Synthesis and Applications of Gold Nanoclusters : (1) Sensing of GSH, (2) Sensing of Mercury(II), and (3) Cluster-Assisted LDI MS.” 2012. Web. 18 Jan 2021.

Vancouver:

Chen T. Novel Synthesis and Applications of Gold Nanoclusters : (1) Sensing of GSH, (2) Sensing of Mercury(II), and (3) Cluster-Assisted LDI MS. [Internet] [Thesis]. NSYSU; 2012. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711112-110624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen T. Novel Synthesis and Applications of Gold Nanoclusters : (1) Sensing of GSH, (2) Sensing of Mercury(II), and (3) Cluster-Assisted LDI MS. [Thesis]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711112-110624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

22. LIN, DING-JYUN. Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione.

Degree: Master, Chemistry, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007

► In this study, we used the advantages of good light stability, broad absorption band, high energy transfer and low biotoxicity, easy surface modification, based fluorescent… (more)

▼ In this study, we used the advantages of good light stability, broad absorption band, high energy transfer and low biotoxicity, easy surface modification, based fluorescent semiconductor polymer nanoparticles (Pdots) to design the detection probe. Using the FRET (FÃ¶rster resonance energy transfer) energy transfer mechanism, combined with Heptamethine cyanine as the radiation near infrared light sensor, developed to the ratiometric method to quantify pH values and quantification of glutathione. (i) Heptamethine cyanine Based Semiconducting Polymer Dots for Detection of Cellular pH. The intracellular pH is very important for cell metabolism, and almost all proteins are based on pH values to maintain their structure and functionality. In addition, pH also plays a very important role in cell proliferation, apoptosis, drug resistance, phagocytosis, endocytosis and signaling. In addition, it has recently been shown that the cytosolic acidity of cells creatine repair is also important. So the intracellular pH is very strict, as long as there is a little abnormal on behalf of some physical problems, such as cancer and Alzheimer's disease, the greater the deviation may even cause death. Therefore, if we can accurately understand and monitoring the entire cell pH in vivo is a very important breakthrough. However, the traditional pH quantitative methods such as electrochemical method because of poor spatial resolution, NMR is limited because of operational difficulties, So we have to develop a faster and more convenient way to detect. Which by fluorescence detection technology, with high resolution, non-destructive, able to design to achieve high selectivity, is currently used in cell research. The ultimate goal of this fluorescent probe is to be able to achieve biological applications, so we use Heptamethine cyanine as a choice of dye, the use of its own structure with a long conjugate chain, modified with a electron-donating functional group based piperazine . The use of it in the acidic environment will be protonated, reducing the ability to donate electrons and extend the conjugate chain to absorb redshift phenomenon. Therefore, in this study, we use the advantage of Pdots easy - to - make the surface modification characteristics, in the polymer side we design of COOH functional groups, and in the near-infrared dye part is modified with NH2 functional groups. The FRET energy transfer mechanism was used to calibrate the pH value of the detective substance. Keywords : Semiconducting polymer dotsãNear-infrared dyesãpH sensingãFRETãFluorescence ratiometric analysis ( ii )Heptamethine cyanine Based Semiconducting Polymer Dots for cellular Quantification of Glutathione GSH is the most abundant bio-mercaptan in cells, the concentration range of 1~15 mmol / L. GSH is involved in many pathology and biology processes and plays an important role in maintaining the balance of intracellular redox states. ROS(reactive oxygen species), and different concentrations of GSH are also associated with a variety of cellular functions, including… Advisors/Committee Members: Ho-Hsiu Chou (chair), Yang-Hsiang Chan (committee member), Ming-Li Tsai (chair).

Subjects/Keywords: Azobenzene; Semiconducting polymer dots; Near-infrared dyes; pH sensing; FRET; Fluorescence ratiometric analysis; GSH sensing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

LIN, D. (2017). Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

LIN, DING-JYUN. “Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione.” 2017. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

LIN, DING-JYUN. “Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione.” 2017. Web. 18 Jan 2021.

Vancouver:

LIN D. Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIN D. Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Yelkenli, İbrahim Halil. Deneysel parkinson modelinde vazoaktif intestinal peptit (VİP)’in nöron koruyucu etkisine astrosit hücrelerinin katkısı .

Degree: ESOGÜ, Tıp Fakültesi, Fizyoloji, 2014, Eskisehir Osmangazi University

URL: http://hdl.handle.net/11684/632

► Parkinson hastalığı, substantia nigradan striatuma gelen dopaminerjik nöron kayıplarına bağlı bazal ganglionların hastalığıdır ve halen etkin bir tedavisi bulunmamaktadır. Tedavi amaçlı uygulanan yöntemler, nöron hasarını… (more)

▼ Parkinson hastalığı, substantia nigradan striatuma gelen dopaminerjik nöron kayıplarına bağlı bazal ganglionların hastalığıdır ve halen etkin bir tedavisi bulunmamaktadır. Tedavi amaçlı uygulanan yöntemler, nöron hasarını durdurma ve yavaşlatmada kısıtlı bir fayda sağlarken, birçok yan etkiye neden olmaktadır. Vazoaktif İntestinal Peptid (VİP) çok yönlü doku koruyucu etkisi nedenli Parkinson hastalığının tedavisinde umut verici bir molekül olarak deneysel Parkinson modellerinde kullanılmıştır. VİP‟in nörodejenerasyonu engellemedeki etki mekanizmasını açığa kavuşturmak için çalışmalar devam etmektedir. Nöron koruyucu etkisini gösterdiği yolaklardan birisinin de astrositler üzerinden olabileceği düşünülmektedir. Astrositler, nöron koruyucu özelliklerini beyin kaynaklı nörotrofik faktör (BDNF), nöron büyüme faktörü (NGF), aktiviteye bağlı nörotrofik protein (ADNP), hidrojen sülfür (H2S), glutatyon (GSH), laktat ve gamma-aminobutirik asit (GABA) gibi bazı molekülleri salıvererek gerçekleştirmektedir. Bunlar arasında astrositlerden ADNP‟nin salıverilmesini uyaran başlıca molekülün de VİP olduğu bildirilmiştir. VİP‟in, parkinson modelinde, nöron koruyucu etkisinde, astrositlerden salıverilen moleküllerle ilişkili çalışmaya rastlanmamıştır. Bu tez çalışmasında, sıçanlarda 6-hidroksidopamin (6-OHDA) verilerek oluşturulan parkinson modelinde VİP‟in nöron koruyucu etki mekanizmasına astrosit hücrelerinin katkısı araştırılmıştır. Deneyler, her iki cinsiyet grubundan Spraque-Dawley sıçanlar kullanılarak üç grupta gerçekleştirilmiştir. 1- Taklit grubu, 2- Parkinson modeli 3- Parkinson modeli oluşturulan ve VİP verilen grup. Sıçanlara motor fonksiyon testini takiben mikrodiyaliz uygulanmıştır. Mikrodiyalizatlarda, GABA, glutamat, GSH ve laktat düzeyleri ölçülmüştür. Mikrodiyaliz işleminden sonra beyinler immunohistokimyasal olarak incelenmiştir. VİP tedavisi, reaktif astrogliozisi etkilemezken, motor fonksiyon bozukluklarını düzeltmiş, GSH düzeylerini kontrol değerlerine geriletmiş, GABA ve glutamat düzeylerini artırmıştır. VİP, striatumda GAD(+) ve ADNP(+) nöron sayısını artırmıştır. 6-OHDA ile oluşturulan nörodejenerasyon sonrasında striatal astrositlerin öncelikle savunmaya yönelik olarak aktive oldukları, VİP‟in nöronları direkt etkileyerek koruduğu sonucuna varılmıştır. Advisors/Committee Members: Tuncel, Neşe (advisor).

Subjects/Keywords: 6-OHDA; Mikrodiyaliz; VİP; GABA; Astrosit; ADNP; GSH; Glutamat; Laktat; Microdialysis; Astrocyte

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yelkenli, �. H. (2014). Deneysel parkinson modelinde vazoaktif intestinal peptit (VİP)’in nöron koruyucu etkisine astrosit hücrelerinin katkısı . (Thesis). Eskisehir Osmangazi University. Retrieved from http://hdl.handle.net/11684/632

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yelkenli, İbrahim Halil. “Deneysel parkinson modelinde vazoaktif intestinal peptit (VİP)’in nöron koruyucu etkisine astrosit hücrelerinin katkısı .” 2014. Thesis, Eskisehir Osmangazi University. Accessed January 18, 2021. http://hdl.handle.net/11684/632.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yelkenli, İbrahim Halil. “Deneysel parkinson modelinde vazoaktif intestinal peptit (VİP)’in nöron koruyucu etkisine astrosit hücrelerinin katkısı .” 2014. Web. 18 Jan 2021.

Vancouver:

Yelkenli �H. Deneysel parkinson modelinde vazoaktif intestinal peptit (VİP)’in nöron koruyucu etkisine astrosit hücrelerinin katkısı . [Internet] [Thesis]. Eskisehir Osmangazi University; 2014. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11684/632.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yelkenli �H. Deneysel parkinson modelinde vazoaktif intestinal peptit (VİP)’in nöron koruyucu etkisine astrosit hücrelerinin katkısı . [Thesis]. Eskisehir Osmangazi University; 2014. Available from: http://hdl.handle.net/11684/632

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Michigan

24. Khobeir, Alexander Mikha. Quantifying the Effects of Common in vitro Cell Culture Techniques on Glutathione Depletion and Cellular Viability via Breast Cancer Cells and Reactive Oxygen Species (ROS).

Degree: MS, Chemistry and Biochemistry, 2016, University of Michigan

URL: http://hdl.handle.net/2027.42/134400

► Glutathione (GSH), an important antioxidant, is essential for proper mammalian biochemical function. Its mechanism as an antioxidant involves the neutralization of reactive oxygen species (ROS)… (more)

Subjects/Keywords: breast cancer; cell culture; glutathione; GSH; in vitro; reactive oxygen species (ROS); biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khobeir, A. M. (2016). Quantifying the Effects of Common in vitro Cell Culture Techniques on Glutathione Depletion and Cellular Viability via Breast Cancer Cells and Reactive Oxygen Species (ROS). (Masters Thesis). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/134400

Chicago Manual of Style (16^th Edition):

Khobeir, Alexander Mikha. “Quantifying the Effects of Common in vitro Cell Culture Techniques on Glutathione Depletion and Cellular Viability via Breast Cancer Cells and Reactive Oxygen Species (ROS).” 2016. Masters Thesis, University of Michigan. Accessed January 18, 2021. http://hdl.handle.net/2027.42/134400.

MLA Handbook (7^th Edition):

Khobeir, Alexander Mikha. “Quantifying the Effects of Common in vitro Cell Culture Techniques on Glutathione Depletion and Cellular Viability via Breast Cancer Cells and Reactive Oxygen Species (ROS).” 2016. Web. 18 Jan 2021.

Vancouver:

Khobeir AM. Quantifying the Effects of Common in vitro Cell Culture Techniques on Glutathione Depletion and Cellular Viability via Breast Cancer Cells and Reactive Oxygen Species (ROS). [Internet] [Masters thesis]. University of Michigan; 2016. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2027.42/134400.

Council of Science Editors:

Khobeir AM. Quantifying the Effects of Common in vitro Cell Culture Techniques on Glutathione Depletion and Cellular Viability via Breast Cancer Cells and Reactive Oxygen Species (ROS). [Masters Thesis]. University of Michigan; 2016. Available from: http://hdl.handle.net/2027.42/134400

Florida International University

25. Wang, Yingsong. Investigating the In Vitro Oxidative Folding Pathways of Bovine Pancreatic Trypsin Inhibitor (BPTI).

Degree: PhD, Chemistry, 2013, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1029 ; 10.25148/etd.FI13121203 ; FI13121203

► The oxidative folding pathway of the disulfide containing protein bovine pancreatic trypsin inhibitor (BPTI) was one of the first to be elucidated and has… (more)

▼ The oxidative folding pathway of the disulfide containing protein bovine pancreatic trypsin inhibitor (BPTI) was one of the first to be elucidated and has served as a basis for understanding the folding pathways of other proteins. During the oxidative folding of reduced BPTI, two intermediates (N' and N*) accumulate in significant amounts and act as kinetic traps. Both N' and N* bury their two remaining free thiols in their hydrophobic cores, which inhibits further oxidation. Historically, the rate limiting step was considered to be the intramolecular rearrangements of N' and N* to another intermediate with two free thiols, N^SH. The two free thiols in N^SH are solvent-exposed and easily oxidized to a disulfide, producing native protein (N). Nevertheless, our research using reduced BPTI indicated that the folding rate of N* to N was proportional to the concentration of added glutathione disulfide (GSSG), inconsistent with the slow intramolecular rearrangement of N* to N^SH. To confirm our initial results, the intermediate N* was purified and refolded in the presence of GSSG. The conversion of N* to N was dependent upon the disulfide concentration and singly mixed disulfide N*(SG) was observed during folding. These results emphasize that the folding of N* can proceed via a growth type pathway, direct oxidation of the two remaining thiols in N* by an exogenous small molecule disulfide, such as GSSG, to form N. Folding of reduced BPTI via N* was performed under changing concentrations of GSSG and GSH as a function of time. The folding was improved dramatically in terms of rate and yield. Aromatic disulfides and thiols have been demonstrated to improve the folding efficiency of disulfide containing proteins including ribonuclease A (RNase A) and lysozyme. Herein, N* and N' were refolded in the presence of aromatic disulfides. Folding of the two kinetic traps with aromatic disulfides indicated that folding proceed via a growth type pathway. The singly and doubly mixed disulfide intermediates were observed during most folding reactions. The oxidative folding of reduced BPTI with aromatic disulfides and thiols were also investigated. Reduced BPTI can be folded to disulfide intermediates rapidly. Advisors/Committee Members: Watson J. Lees, Konstantinos Kavallieratos, Lidia Kos, Kevin O'Shea, Xiaotang Wang.

Subjects/Keywords: BPTI; folding pathway; protein folding; GSSG and GSH; aromatic disulfides; HPLC; Biochemistry; Other Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Y. (2013). Investigating the In Vitro Oxidative Folding Pathways of Bovine Pancreatic Trypsin Inhibitor (BPTI). (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1029 ; 10.25148/etd.FI13121203 ; FI13121203

Chicago Manual of Style (16^th Edition):

Wang, Yingsong. “Investigating the In Vitro Oxidative Folding Pathways of Bovine Pancreatic Trypsin Inhibitor (BPTI).” 2013. Doctoral Dissertation, Florida International University. Accessed January 18, 2021. https://digitalcommons.fiu.edu/etd/1029 ; 10.25148/etd.FI13121203 ; FI13121203.

MLA Handbook (7^th Edition):

Wang, Yingsong. “Investigating the In Vitro Oxidative Folding Pathways of Bovine Pancreatic Trypsin Inhibitor (BPTI).” 2013. Web. 18 Jan 2021.

Vancouver:

Wang Y. Investigating the In Vitro Oxidative Folding Pathways of Bovine Pancreatic Trypsin Inhibitor (BPTI). [Internet] [Doctoral dissertation]. Florida International University; 2013. [cited 2021 Jan 18]. Available from: https://digitalcommons.fiu.edu/etd/1029 ; 10.25148/etd.FI13121203 ; FI13121203.

Council of Science Editors:

Wang Y. Investigating the In Vitro Oxidative Folding Pathways of Bovine Pancreatic Trypsin Inhibitor (BPTI). [Doctoral Dissertation]. Florida International University; 2013. Available from: https://digitalcommons.fiu.edu/etd/1029 ; 10.25148/etd.FI13121203 ; FI13121203

University of Helsinki

26. Gull, Yvonne. Xenobiotisk metabolism genom cytokrom P-450, glutationkonjugering och glukuronidering samt effekt av OR-1384 på leverns xenobiotiska metabolism;kombination med fenobarbital.

Degree: 1996, University of Helsinki

URL: http://hdl.handle.net/10138/158666

Subjects/Keywords: OR-1384; fenobarbital; xenobiotisk metabolism; cytokrom P-450; 7-etoxykumarin O-deetylas; glutation; GSH-transferas; GSH-reduktas; GSH-peroxidas; UDP-glukuronosyltransferas; IBD; Farmakologia; OR-1384; fenobarbital; xenobiotisk metabolism; cytokrom P-450; 7-etoxykumarin O-deetylas; glutation; GSH-transferas; GSH-reduktas; GSH-peroxidas; UDP-glukuronosyltransferas; IBD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gull, Y. (1996). Xenobiotisk metabolism genom cytokrom P-450, glutationkonjugering och glukuronidering samt effekt av OR-1384 på leverns xenobiotiska metabolism;kombination med fenobarbital. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/158666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gull, Yvonne. “Xenobiotisk metabolism genom cytokrom P-450, glutationkonjugering och glukuronidering samt effekt av OR-1384 på leverns xenobiotiska metabolism;kombination med fenobarbital.” 1996. Thesis, University of Helsinki. Accessed January 18, 2021. http://hdl.handle.net/10138/158666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gull, Yvonne. “Xenobiotisk metabolism genom cytokrom P-450, glutationkonjugering och glukuronidering samt effekt av OR-1384 på leverns xenobiotiska metabolism;kombination med fenobarbital.” 1996. Web. 18 Jan 2021.

Vancouver:

Gull Y. Xenobiotisk metabolism genom cytokrom P-450, glutationkonjugering och glukuronidering samt effekt av OR-1384 på leverns xenobiotiska metabolism;kombination med fenobarbital. [Internet] [Thesis]. University of Helsinki; 1996. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/10138/158666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gull Y. Xenobiotisk metabolism genom cytokrom P-450, glutationkonjugering och glukuronidering samt effekt av OR-1384 på leverns xenobiotiska metabolism;kombination med fenobarbital. [Thesis]. University of Helsinki; 1996. Available from: http://hdl.handle.net/10138/158666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

27. Kang, Cheng-yang. Studies on the Enhancement of Chilling Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile.

Degree: Master, Marine Biology, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-014657

► The purpose of this study is to construct the gene of glutathione reductase (UfGr) of marine macroalga Ulva fascitat Delile following with GFP gene in… (more)

Subjects/Keywords: Oryza sativa; GSH (reduced glutathione); GR (Glutathione reductase); transformation; oxidative stress; salt stress; low temperature stress

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APA (6^th Edition):

Kang, C. (2013). Studies on the Enhancement of Chilling Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-014657

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kang, Cheng-yang. “Studies on the Enhancement of Chilling Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile.” 2013. Thesis, NSYSU. Accessed January 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-014657.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kang, Cheng-yang. “Studies on the Enhancement of Chilling Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile.” 2013. Web. 18 Jan 2021.

Vancouver:

Kang C. Studies on the Enhancement of Chilling Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-014657.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kang C. Studies on the Enhancement of Chilling Tolerance of Rice (Oryza sativa L.) Seedlings by Overexpression of Glutathione Reductase from Marine Macroalga Ulva fasciata Delile. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630113-014657

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

28. Shi, Shujie. Identification of Human Gene Products Containing Pro-Pro-x-Tyr (PY) Motifs That Enhance Glutathione and Endocytotic Markers Uptake in Yeast.

Degree: PhD, 2010, University of Rochester

URL: http://hdl.handle.net/1802/10503

► Glutathione (GSH) plays essential roles in many cellular processes, and disturbances in its homeostasis are associated with a number of human diseases. GSH export from… (more)

▼ Glutathione (GSH) plays essential roles in many cellular processes, and disturbances in its homeostasis are associated with a number of human diseases. GSH export from cells is a key component in regulating GSH levels; however the proteins that mediate GSH transport and underlying mechanisms are not well defined. In an attempt to identify novel GSH transporters, a human mammary gland cDNA library was screened for clones capable of complementing GSH uptake by yeast cells that lack Hgt1p, the primary GSH uptake transporter in yeast, although these cells do retain an uncharacterized low affinity GSH uptake mechanism. This screening strategy initially identified 11 cDNAs that complement the growth deficiency of the HGT1 deletion cells in sulfur-deficient medium supplemented with GSH. From this pool, five human genes were identified that were able to increase intracellular glutathione levels, namely: PMEPA1, prostate transmembrane protein, androgen induced 1; LAPTM4α, lysosomal-associated protein transmembrane 4 alpha; SLC25A1, solute carrier family 25, member 1; LITAF, lipopolysaccharide-induced TNF factor; and CYYR1, cysteine/tyrosine-rich-1. All of these genes encode for small integral membrane proteins of unknown function, although none appear to encode for prototypical GSH transporters. Nevertheless, they all increased [3H]GSH uptake by the yeast cells. The enhanced [3H]GSH uptake rate was uniformly inhibited by high concentrations of unlabeled GSH, GSSG, and ophthalmic acid, suggesting that it is due to increased activity of an endogenous yeast low affinity GSH uptake mechanism. Interestingly, each protein is predicted to contain Pro-Pro-x-Tyr (PY) motifs, which are thought to be important for regulating intracellular protein sorting, plasma membrane protein expression, and multiple steps of ubiquitin-dependent endocytosis. Indeed, uptake of the endocytotic markers lucifer yellow and FM4-64 was enhanced by each of the five genes. Mutations of the PY motifs in LITAF largely abolished all of its effects. In summary, although the results do not reveal novel GSH transporters, they identify five PY-containing gene products that enhance GSH and endocytotic marker uptake in yeast, suggesting that these proteins are involved in cell surface remodeling. In addition, a HGT1 overexpressing yeast strain was characterized. The constitutive expression of Hgt1p led to increased GSH uptake and excessive GSH accumulation in the yeast. As a result of GSH toxicity, these cells exhibited growth inhibition in GSH containing medium, and thus could potentially be utilized as a model to search for unidirectional GSH efflux transporters.

Subjects/Keywords: GSH; Py; Endocytosis; Yeast; Membrane Turnover; Transporters

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APA (6^th Edition):

Shi, S. (2010). Identification of Human Gene Products Containing Pro-Pro-x-Tyr (PY) Motifs That Enhance Glutathione and Endocytotic Markers Uptake in Yeast. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/10503

Chicago Manual of Style (16^th Edition):

Shi, Shujie. “Identification of Human Gene Products Containing Pro-Pro-x-Tyr (PY) Motifs That Enhance Glutathione and Endocytotic Markers Uptake in Yeast.” 2010. Doctoral Dissertation, University of Rochester. Accessed January 18, 2021. http://hdl.handle.net/1802/10503.

MLA Handbook (7^th Edition):

Shi, Shujie. “Identification of Human Gene Products Containing Pro-Pro-x-Tyr (PY) Motifs That Enhance Glutathione and Endocytotic Markers Uptake in Yeast.” 2010. Web. 18 Jan 2021.

Vancouver:

Shi S. Identification of Human Gene Products Containing Pro-Pro-x-Tyr (PY) Motifs That Enhance Glutathione and Endocytotic Markers Uptake in Yeast. [Internet] [Doctoral dissertation]. University of Rochester; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1802/10503.

Council of Science Editors:

Shi S. Identification of Human Gene Products Containing Pro-Pro-x-Tyr (PY) Motifs That Enhance Glutathione and Endocytotic Markers Uptake in Yeast. [Doctoral Dissertation]. University of Rochester; 2010. Available from: http://hdl.handle.net/1802/10503

University of Illinois – Urbana-Champaign

29. Leslie, Matthew Thomas. Microenvironmental and metabolic influences in tumor cell redox homeostasis.

Degree: PhD, VMS - Pathobiology, 2017, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/101106

► Redox reactions dictate protein shape, regulate enzyme activity, and underscore cellular metabolism. When redox poise fails and cells are overcome by oxidation, cellular death soon… (more)

▼ Redox reactions dictate protein shape, regulate enzyme activity, and underscore cellular metabolism. When redox poise fails and cells are overcome by oxidation, cellular death soon follows. Redox biology is an especially important aspect of cancer cell biology, because oncogene-induced hyperproliferation results in pro-oxidant tumor cell phenotypes. Thus, tumor cell antioxidant systems are often inherently strained and vulnerable to further oxidative attack. Targeted therapies can be designed to kill tumor cells by pushing them beyond redox thresholds compatible with cellular life without injuring healthy cells that can more effectively dissipate oxidative stress. Radiation therapy and many chemotherapeutic drugs use this mechanism of oxidant-based cell killing to eliminate tumor cell populations. However, in clinical settings, these therapies face tumor cell resistance stemming from multifactorial changes in cellular drug and energy metabolism, environmental selective pressures, and intratumoral heterogeneity. To further advance efficacious and selective anticancer strategies, increased understanding of microenvironmental and metabolic influences in tumor cells redox homeostasis is needed. Poorly arranged and malfunctioning vasculature in the tumor microenvironment causes heterogeneous regions of hypoxia to form throughout the solid mass. Clinically, tumor hypoxia is a negative prognostic indicator for a more aggressive phenotype and is associated with treatment resistance. Metabolically, tumor hypoxia results in hypoxia inducible factor-driven gene network activation and enhanced mitochondrial production of reactive oxygen species. Given this, it is important to match laboratory in vitro studies as closely as possible to the in vivo conditions of the tumor microenvironment. However, even simple in vitro hypoxic studies are complicated by the rigors of hypoxic experimentation and, thus, most studies are conducted at hyperoxic atmospheric levels of oxygen. Engineered microfluidic platforms offer an approach to hypoxic laboratory studies. This report validates an ease-of-use platform suitable to study complex cell behaviors in hypoxia. Herein we present the design and implementation of an open-well microfluidic platform that enables on-chip cell culture, experimentation, and microscopy in a controlled hypoxic microenvironment. This platform has been theoretically and experimentally validated to quickly induce and maintain 0.3 mg/L O2 hypoxia, and it has enabled studies of the stabilization of hypoxia inducible factor in hypoxia and the redox response of the mitochondrial matrix in hypoxia. The glutathione system functions in antioxidant defense and xenobiotic detoxification to preserve subcellular redox poise and maintain enzymes in functional reduced states. Glutathione is produced from its constitutive amino acids in a two-step enzymatic process that occurs in the cytosol. Despite glutathione’s role in cellular antioxidant defense, its production in the pro-oxidant state of hypoxia has been under studied. We… Advisors/Committee Members: Gaskins, H. Rex (advisor), Gaskins, H. Rex (Committee Chair), Kenis, Paul (committee member), Kulenschmidt, Mark (committee member), Lau, Gee (committee member), Fan, Tim (committee member), Grippo, Paul (committee member).

Subjects/Keywords: Cancer; RAS Oncogene; Metabolism; Glutaminolysis; Glutamine; Alpha-ketoglutarate; Glutathione; GSH; Redox; Reactive Oxygen Species; Hypoxia; Microfluidics; roGFP2 biosensor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leslie, M. T. (2017). Microenvironmental and metabolic influences in tumor cell redox homeostasis. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101106

Chicago Manual of Style (16^th Edition):

Leslie, Matthew Thomas. “Microenvironmental and metabolic influences in tumor cell redox homeostasis.” 2017. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 18, 2021. http://hdl.handle.net/2142/101106.

MLA Handbook (7^th Edition):

Leslie, Matthew Thomas. “Microenvironmental and metabolic influences in tumor cell redox homeostasis.” 2017. Web. 18 Jan 2021.

Vancouver:

Leslie MT. Microenvironmental and metabolic influences in tumor cell redox homeostasis. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2142/101106.

Council of Science Editors:

Leslie MT. Microenvironmental and metabolic influences in tumor cell redox homeostasis. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/101106

Harvard University

30. Romero, Catalina. Spatio-temporal control of the cytosolic redox environment in C. elegans.

Degree: PhD, Systems Biology, 2013, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11181131

► Compartmentalization of redox reactions is essential to all life forms. Protein activity can respond to changes in the local redox environment through the reversible oxidation… (more)

Subjects/Keywords: Molecular biology; Cellular biology; Genetics; aging; C. elegans; insulin signaling; oxidation reduction of proteins; redox potential of glutathione (GSH); spatial patterning

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Romero, C. (2013). Spatio-temporal control of the cytosolic redox environment in C. elegans. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:11181131

Chicago Manual of Style (16^th Edition):

Romero, Catalina. “Spatio-temporal control of the cytosolic redox environment in C. elegans.” 2013. Doctoral Dissertation, Harvard University. Accessed January 18, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11181131.

MLA Handbook (7^th Edition):

Romero, Catalina. “Spatio-temporal control of the cytosolic redox environment in C. elegans.” 2013. Web. 18 Jan 2021.

Vancouver:

Romero C. Spatio-temporal control of the cytosolic redox environment in C. elegans. [Internet] [Doctoral dissertation]. Harvard University; 2013. [cited 2021 Jan 18]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11181131.

Council of Science Editors:

Romero C. Spatio-temporal control of the cytosolic redox environment in C. elegans. [Doctoral Dissertation]. Harvard University; 2013. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11181131

Open Access Theses and Dissertations