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NSYSU

1. LIN, DING-JYUN. Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione.

Degree: Master, Chemistry, 2017, NSYSU

In this study, we used the advantages of good light stability, broad absorption band, high energy transfer and low biotoxicity, easy surface modification, based fluorescent semiconductor polymer nanoparticles (Pdots) to design the detection probe. Using the FRET (Förster resonance energy transfer) energy transfer mechanism, combined with Heptamethine cyanine as the radiation near infrared light sensor, developed to the ratiometric method to quantify pH values and quantification of glutathione. (i) Heptamethine cyanine Based Semiconducting Polymer Dots for Detection of Cellular pH. The intracellular pH is very important for cell metabolism, and almost all proteins are based on pH values to maintain their structure and functionality. In addition, pH also plays a very important role in cell proliferation, apoptosis, drug resistance, phagocytosis, endocytosis and signaling. In addition, it has recently been shown that the cytosolic acidity of cells creatine repair is also important. So the intracellular pH is very strict, as long as there is a little abnormal on behalf of some physical problems, such as cancer and Alzheimer's disease, the greater the deviation may even cause death. Therefore, if we can accurately understand and monitoring the entire cell pH in vivo is a very important breakthrough. However, the traditional pH quantitative methods such as electrochemical method because of poor spatial resolution, NMR is limited because of operational difficulties, So we have to develop a faster and more convenient way to detect. Which by fluorescence detection technology, with high resolution, non-destructive, able to design to achieve high selectivity, is currently used in cell research. The ultimate goal of this fluorescent probe is to be able to achieve biological applications, so we use Heptamethine cyanine as a choice of dye, the use of its own structure with a long conjugate chain, modified with a electron-donating functional group based piperazine . The use of it in the acidic environment will be protonated, reducing the ability to donate electrons and extend the conjugate chain to absorb redshift phenomenon. Therefore, in this study, we use the advantage of Pdots easy - to - make the surface modification characteristics, in the polymer side we design of COOH functional groups, and in the near-infrared dye part is modified with NH2 functional groups. The FRET energy transfer mechanism was used to calibrate the pH value of the detective substance. Keywords : Semiconducting polymer dotsãNear-infrared dyesãpH sensingãFRETãFluorescence ratiometric analysis ( ii )Heptamethine cyanine Based Semiconducting Polymer Dots for cellular Quantification of Glutathione GSH is the most abundant bio-mercaptan in cells, the concentration range of 1~15 mmol / L. GSH is involved in many pathology and biology processes and plays an important role in maintaining the balance of intracellular redox states. ROS(reactive oxygen species), and different concentrations of GSH are also associated with a variety of cellular functions, including… Advisors/Committee Members: Ho-Hsiu Chou (chair), Yang-Hsiang Chan (committee member), Ming-Li Tsai (chair).

Subjects/Keywords: Azobenzene; Semiconducting polymer dots; Near-infrared dyes; pH sensing; FRET; Fluorescence ratiometric analysis; GSH sensing

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APA (6th Edition):

LIN, D. (2017). Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

LIN, DING-JYUN. “Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione.” 2017. Thesis, NSYSU. Accessed November 24, 2017. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

LIN, DING-JYUN. “Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione.” 2017. Web. 24 Nov 2017.

Vancouver:

LIN D. Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione. [Internet] [Thesis]. NSYSU; 2017. [cited 2017 Nov 24]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIN D. Heptamethine Cyanine Based Semiconducting Polymer Dots for : ( i ) Detection of Cellular pH ; and ( ii ) Quantification of Glutathione. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806117-224007

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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