subject:(GCF)

1. 小野, 裕貴. 歯肉溝滲出液中のTannerella forsythia Forsythia Detaching Factor に対する切断酵素活性の治療効果判定への応用 : Application of Proteolytic Activity toward Tannerella forsythia Forsythia Detaching Factor in Gingival Crevicular Fluid to Determination of Therapeutic Effect.

Degree: 博士（歯学）, 2015, Meikai　University / 明海大学

URL: http://id.nii.ac.jp/1216/00000053/

2014

Subjects/Keywords: Tannerella forsythia; forsythia detaching factor; FC activity; GCF; Tannerella forsythia; forsythia detaching factor; FC activity; GCF

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

小野, �. (2015). 歯肉溝滲出液中のTannerella forsythia Forsythia Detaching Factor に対する切断酵素活性の治療効果判定への応用 : Application of Proteolytic Activity toward Tannerella forsythia Forsythia Detaching Factor in Gingival Crevicular Fluid to Determination of Therapeutic Effect. (Thesis). Meikai　University / 明海大学. Retrieved from http://id.nii.ac.jp/1216/00000053/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

小野, 裕貴. “歯肉溝滲出液中のTannerella forsythia Forsythia Detaching Factor に対する切断酵素活性の治療効果判定への応用 : Application of Proteolytic Activity toward Tannerella forsythia Forsythia Detaching Factor in Gingival Crevicular Fluid to Determination of Therapeutic Effect.” 2015. Thesis, Meikai　University / 明海大学. Accessed April 17, 2021. http://id.nii.ac.jp/1216/00000053/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

小野, 裕貴. “歯肉溝滲出液中のTannerella forsythia Forsythia Detaching Factor に対する切断酵素活性の治療効果判定への応用 : Application of Proteolytic Activity toward Tannerella forsythia Forsythia Detaching Factor in Gingival Crevicular Fluid to Determination of Therapeutic Effect.” 2015. Web. 17 Apr 2021.

Vancouver:

小野 �. 歯肉溝滲出液中のTannerella forsythia Forsythia Detaching Factor に対する切断酵素活性の治療効果判定への応用 : Application of Proteolytic Activity toward Tannerella forsythia Forsythia Detaching Factor in Gingival Crevicular Fluid to Determination of Therapeutic Effect. [Internet] [Thesis]. Meikai　University / 明海大学; 2015. [cited 2021 Apr 17]. Available from: http://id.nii.ac.jp/1216/00000053/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

小野 �. 歯肉溝滲出液中のTannerella forsythia Forsythia Detaching Factor に対する切断酵素活性の治療効果判定への応用 : Application of Proteolytic Activity toward Tannerella forsythia Forsythia Detaching Factor in Gingival Crevicular Fluid to Determination of Therapeutic Effect. [Thesis]. Meikai　University / 明海大学; 2015. Available from: http://id.nii.ac.jp/1216/00000053/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Τυροβολά, Ιωάννα. Συσχέτιση επιπέδων SRANKLκαι OPG στον ορό του αίματος και στο υγρό της ουλοδοντικής σχισμής και βαθμού ριζικής απορρόφησης κατά την ορθοδοντική θεραπεία.

Degree: 2009, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/27799

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The aim of the present study was the determination in a rat model of the levels of OPG and sRANKL in blood and in gingival… (more)

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The aim of the present study was the determination in a rat model of the levels of OPG and sRANKL in blood and in gingival crevicular fluid (GCF) in relation to the degree of root resorption during orthodontic tooth movement using healthy experimental animals and a carefully planned and organized experimental design. For this purpose fourteen 6 month old male Wistar rats 350-500 gr weight were used. At a first stage blood samples from the area of the eyes were taken and gingival crevicular fluid (GCF) was collected with the use of paperstrips (penopaper Harco Tustin, CA, USA) from the gingival crevicular area of each upper incisor. Blood samples were also collected after the orthodontic treatment had been completed. A 25gr sentalloy closed orthodontic coil spring(GAC, Ctr Iship, NY, USA) was inserted between the upper right first molar and the upper incisors in all animals. After 21 days of loading a mesial tipping of the right molars was obtained. All night first molars that were moved orthodontically and their cognate left molars (control teeth) were extracted and studied under microcomputed tomography scanning. After studying the extracted teeth the following results were obtained: a) there is a positive linear correlation between the initial concentration of RANKL in blood and the degree of root resorption (r=0.833, p<0.01), b) the ratio of the initial concentrations of OPG/RANKL in blood proved to be an independent prognostic factor of the degree of root resorption after orthodontic therapy (R²=0.87, Adj R²=0.73, Beta=0.872, p<0.01), c) the initial concentration of RANKL in GCF showed a negative correlation to the initial concentration of RANKL in blood serum (r=-0.7, p<0.05), d) it seems that for a concrete group of initial concentrations of OPG/RANKL in GCF, the dental root is protected against extreme external root resorption (R=0.81, p<0.05 for x² η R=0.83, p<0.05 for x³) and finally, e) the concentration of OPG in blood serum decreased significantly in cases of severe root resorption (r=-0.823, p=0.001).

Σκοπός της παρούσας ερευνητικής εργασίας ήταν η διερεύνηση της σχέσεως των επιπέδων της OPG και του s RANKL στο αίμα και στο υγρό της ουλοδοντικής σχισμής σε σχέση με το βαθμό της ριζικής απορρόφησης κατά την ορθοδοντική μετακίνηση των δοντιών. Στην ερευνητική μας εργασία χρησιμοποιήθηκαν 14 υγιείς επίμυες τύπου Wistar 350-500 γρ. περίπου ηλικίας 6 μηνών και όλοι αρσενικού φύλου. Σε κάθε ζώο λήφθηκε δείγμα αίματος πριν και μετά την ορθοδοντική παρέμβαση καθώς επίσης και διπλό (σε ορισμένες περιπτώσεις όπου οι περιοδοντικοί ιστοί δεν ερεθίστηκαν πολύ) δείγμα υγρού από την ουλοδοντική σχισμή του τομέα πριν την ορθοδοντική παρέμβαση. Μετά εφαρμόσθηκε μηχανισμός για την ορθοδοντική μετακίνηση κάθε δεξιού άνω πρώτου γομφίου προς τα εγγύς. Στην αντίθετη πλευρά δηλαδή στην περιοχή του πρώτου άνω γομφίου αριστερά δεν εφαρμόσθηκαν καθόλου ορθοδοντικές δυνάμεις (δόντι έλεγχου). Η ορθοδοντική θεραπεία είχε διάρκεια τριών εβδομάδων. Ακολούθησε αξονική τομογραφία τόσο των δοντιών παρέμβασης όσο και των δοντιών…

Subjects/Keywords: Οστεοπροτεγερίνη; Ριζική απορρόφηση; Ορθοδοντική θεραπεία; Υγρό ουλοδοντικής σχισμής; Ορός αίματος; Οστική αναδιαμόρφωση; Απόπτωση νεογιλού; Πρωτεΐνες; Osteoprotegerin (OPG); Receptor activator of NF-KB ligand (RANKL); Receptor activator of NF-KB (RANK); Root resorption; Orthodontic treatment; Blood serum; Gingival crevicular fluid (GCF); Proteins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Τυροβολά, . �. (2009). Συσχέτιση επιπέδων SRANKLκαι OPG στον ορό του αίματος και στο υγρό της ουλοδοντικής σχισμής και βαθμού ριζικής απορρόφησης κατά την ορθοδοντική θεραπεία. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/27799

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Τυροβολά, Ιωάννα. “Συσχέτιση επιπέδων SRANKLκαι OPG στον ορό του αίματος και στο υγρό της ουλοδοντικής σχισμής και βαθμού ριζικής απορρόφησης κατά την ορθοδοντική θεραπεία.” 2009. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 17, 2021. http://hdl.handle.net/10442/hedi/27799.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Τυροβολά, Ιωάννα. “Συσχέτιση επιπέδων SRANKLκαι OPG στον ορό του αίματος και στο υγρό της ουλοδοντικής σχισμής και βαθμού ριζικής απορρόφησης κατά την ορθοδοντική θεραπεία.” 2009. Web. 17 Apr 2021.

Vancouver:

Τυροβολά �. Συσχέτιση επιπέδων SRANKLκαι OPG στον ορό του αίματος και στο υγρό της ουλοδοντικής σχισμής και βαθμού ριζικής απορρόφησης κατά την ορθοδοντική θεραπεία. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10442/hedi/27799.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Τυροβολά �. Συσχέτιση επιπέδων SRANKLκαι OPG στον ορό του αίματος και στο υγρό της ουλοδοντικής σχισμής και βαθμού ριζικής απορρόφησης κατά την ορθοδοντική θεραπεία. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. Available from: http://hdl.handle.net/10442/hedi/27799

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Boräng, Jennifer. Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis.

Degree: 2012, , Faculty of Odontology (OD)

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944

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Kronisk parodontit orsakar vävnadsdestruktion till följd av matrixmetalloproteinasaktivitet. Dessa enzym härrör från värdcellerna och är en del av det immunologiska svaret på bakteriella virulensfaktorer.… (more)

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Kronisk parodontit orsakar vävnadsdestruktion till följd av matrixmetalloproteinasaktivitet. Dessa enzym härrör från värdcellerna och är en del av det immunologiska svaret på bakteriella virulensfaktorer. Grönt te har studerats för sina hälsofrämjande egenskaper, som omfattar bland annat anti-inflammatoriska effekter. Effekten beror delvis på enzyminhibering av tepolyfenoler. Syftet med denna studie var att ytterligare undersöka den inhiberande effekten av grönt te, med fokus på enzymatisk aktivitet i gingivalvätska från patienter med parodontal sjukdom. Patienter med kronisk parodontit valdes ut för att delta i studien. Gingivalvätska extraherades med mikropipetter från patienternas gingivala sulci. Proverna behandlades med grönt te och jämfördes med obehandlade prover från samma försöksperson. Fluorescens proteasanalys med kasein som substrat utfördes på fjorton prover för att detektera skillnader i kaseinolytisk aktivitet. Zymogramanalys med användning av gelatin som substrat utfördes på fyra prover, för att undersöka skillnader i gelatinolytisk aktivitet och analysera molekylvikter för de olika enzymerna. Den fluorometriska analysen visade en signifikant lägre enzymaktivitet i prover med tillsatt grönt te jämfört med obehandlade prover (p<0.001). Zymogramanalysen visade en skillnad i enzymaktivitet som var mest uttalad i banden för molekylär vikt runt 255 kDa, analogt med komplex av matrixmetalloproteinas-9. Sammanfattningsvis har det i denna studie påvisats att grönt te har en hämmande effekt på kaseinolytisk aktivitet och en mindre, mer specifik, hämmande effekt på gelatinasaktivitet.

Chronic periodontitis involves tissue destruction by matrix metalloproteinase, derived from the host cells, as part of the immunological response to bacterial virulence factors. Green tea has been studied for its health promoting properties, which includes anti-inflammatory effects. The effect is in part due to enzyme inhibition by tea polyphenols. The aim of this study was to further investigate the inhibitory effect of green tea, focusing on enzymatic activity in gingival crevicular fluid from patients with periodontal disease. Patients with chronic periodontitis were selected for participation in the study. Gingival crevicular fluid was extracted with micropipettes from the gingival sulci of the patients. Samples were treated with green tea and compared with untreated samples from the same subject. Fluorescence protease assay with casein as substrate was made using fourteen samples for detecting differences in caseinolytic activity. Zymogram assay using gelatin as substrate was done using four samples to test gelatinolytic activity and analyse molecular weights of the different enzymes. The fluorometric assay showed a significantly lower enzyme activity in samples mixed with green tea than untreated samples (p<0.001). The zymogram assay showed a difference in band strength which was most pronounced in the bands of molecular weight around 255 kDA, analogous to complexes of matrix metalloproteinase-9. In conclusion,…

Subjects/Keywords: green tea; polyphenols; EGCg; GCF; gingival crevicular fluid; periodontitis; MMP; inhibition; enzyme; fluorescence assay; zymography; Medical and Health Sciences; Medicin och hälsovetenskap

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boräng, J. (2012). Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis. (Thesis). , Faculty of Odontology (OD). Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boräng, Jennifer. “Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis.” 2012. Thesis, , Faculty of Odontology (OD). Accessed April 17, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boräng, Jennifer. “Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis.” 2012. Web. 17 Apr 2021.

Vancouver:

Boräng J. Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis. [Internet] [Thesis]. , Faculty of Odontology (OD); 2012. [cited 2021 Apr 17]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boräng J. Green tea inhibits proteolytic enzymes in GCF from patients with chronic periodontitis. [Thesis]. , Faculty of Odontology (OD); 2012. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19944

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Chien, Ming. Comparison of Anti-inflammatory Effects Produced in Gingiva by Metronidazole and Azithromycin.

Degree: MS, Dentistry, 2014, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1402670626

► Objective: Previous studies suggest that azithromycin (AZM) inhibits subclinical gingi-val inflammation in individuals with minimal plaque and clinically healthy gingiva. While it is unclear whether… (more)

▼ Objective: Previous studies suggest that azithromycin (AZM) inhibits subclinical gingi-val inflammation in individuals with minimal plaque and clinically healthy gingiva. While it is unclear whether other antibiotics produce similar effects in gingiva, metroni-dazole (MET) has been shown to decrease pro-inflammatory cytokine production by cul-tured periodontal ligament cells. This randomized, blinded, crossover study compared anti-inflammatory effects produced by AZM and MET.Methods: Twelve healthy adult subjects with good oral hygiene and clinically healthy gingiva were randomly allocated to receive a blinded regimen of either AZM (500mg ini-tial dose, then 250mg at 24 and 48 hrs) or MET (375mg every 12 hr for 48 hr). At base-line (immediately before starting the regimens) and 2, 4, 7 and 14 days later, gingival crevicular fluid (GCF) samples were collected from twelve maxillary interproximal sites, measured with a calibrated Periotron, pooled, and stored frozen. After a 21 day washout period, each subject received the alternative regimen. A second set of GCF samples were collected at the same time points. GCF samples were assayed for pro- and anti-inflammatory cytokine signatures by multiplex immunoassay.Results: Both agents induced significant transient decreases in the rate of GCF flow on days 2, 4 and 7. No significant differences in their effects were observed. With both an-tibiotics, the GCF flow rate increased to approximately 86% of baseline between days 7 and 14. In parallel with their effects on GCF volume, AZM and MET induced transient reductions in the GCF content of several pro-inflammatory cytokines (IL-1ß, IL-12, IL-17, G-CSF) as well as IL-8, RANTES, VEGF, and IL-4. The effects of AZM and MET were similar in magnitude and the most significant decreases below baseline levels were apparent on days 4 and 7. MET induced a larger decrease in GCF IL-8 content than AZM. Neither agent produced significant changes in the amounts of the anti-inflammatory cytokines IL-10 and IL-1 receptor antagonist in GCF. Conclusions: In addition to their known antimicrobial effects, AZM and MET both ap-pear to inhibit production of a similar range of pro-inflammatory cytokines and chemoki-nes in gingiva. This could potentially enhance the efficacy of these agents in the treat-ment of inflammatory periodontitis. Advisors/Committee Members: Walters, John (Advisor).

Subjects/Keywords: Dentistry; Periodontics; Antibiotics; Azithromycin; Metronidazole; GCF; Periodontitis; healthy gingiva

…Subject Groups �� 14 Table 2: Changes in GCF Biological Mediator Content Induced… …allocation �� .17 Figure 2: Effects of systemic AZM and MET on pooled GCF volume �� ….18… …Figure 3: Effects of systemic AZM and MET on the GCF content of IL-1β and G-CSF.19 Figure 4… …Effects of AZM and MET on the GCF content of IL-12 (p70) and IL-17 ��.20 Figure 5… …Effects of AZM and MET on GCF IL-8 and RANTES content �� 21 Figure 6: Effects of systemic AZM…

5 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chien, M. (2014). Comparison of Anti-inflammatory Effects Produced in Gingiva by Metronidazole and Azithromycin. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1402670626

Chicago Manual of Style (16^th Edition):

Chien, Ming. “Comparison of Anti-inflammatory Effects Produced in Gingiva by Metronidazole and Azithromycin.” 2014. Masters Thesis, The Ohio State University. Accessed April 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1402670626.

MLA Handbook (7^th Edition):

Chien, Ming. “Comparison of Anti-inflammatory Effects Produced in Gingiva by Metronidazole and Azithromycin.” 2014. Web. 17 Apr 2021.

Vancouver:

Chien M. Comparison of Anti-inflammatory Effects Produced in Gingiva by Metronidazole and Azithromycin. [Internet] [Masters thesis]. The Ohio State University; 2014. [cited 2021 Apr 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1402670626.

Council of Science Editors:

Chien M. Comparison of Anti-inflammatory Effects Produced in Gingiva by Metronidazole and Azithromycin. [Masters Thesis]. The Ohio State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1402670626

University of Toronto

5. Ford, Heather Nicole. Nitric Oxide Changes in Gingival Crevicular Fluid Following Orthodontic Force Application.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/42838

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Nitric oxide (NO) plays a role in regulating the rate of orthodontic tooth movement (OTM) in rat models; however, in humans this role remains less… (more)

Subjects/Keywords: gingival crevicular fluid; griess reaction; GCF; orthodontic force application; nitric oxide; 0567

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ford, H. N. (2013). Nitric Oxide Changes in Gingival Crevicular Fluid Following Orthodontic Force Application. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/42838

Chicago Manual of Style (16^th Edition):

Ford, Heather Nicole. “Nitric Oxide Changes in Gingival Crevicular Fluid Following Orthodontic Force Application.” 2013. Masters Thesis, University of Toronto. Accessed April 17, 2021. http://hdl.handle.net/1807/42838.

MLA Handbook (7^th Edition):

Ford, Heather Nicole. “Nitric Oxide Changes in Gingival Crevicular Fluid Following Orthodontic Force Application.” 2013. Web. 17 Apr 2021.

Vancouver:

Ford HN. Nitric Oxide Changes in Gingival Crevicular Fluid Following Orthodontic Force Application. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1807/42838.

Council of Science Editors:

Ford HN. Nitric Oxide Changes in Gingival Crevicular Fluid Following Orthodontic Force Application. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/42838

University of Adelaide

6. Kobayashi, Kere. Expression of bone resorption markers in gingival crevicular fluid of rheumatoid arthritis patients with and without periodontitis: a pilot study.

Degree: 2012, University of Adelaide

URL: http://hdl.handle.net/2440/77344

Subjects/Keywords: periodontitis; periodontal; rheumatoid arthritis; GCF

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kobayashi, K. (2012). Expression of bone resorption markers in gingival crevicular fluid of rheumatoid arthritis patients with and without periodontitis: a pilot study. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/77344

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kobayashi, Kere. “Expression of bone resorption markers in gingival crevicular fluid of rheumatoid arthritis patients with and without periodontitis: a pilot study.” 2012. Thesis, University of Adelaide. Accessed April 17, 2021. http://hdl.handle.net/2440/77344.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kobayashi, Kere. “Expression of bone resorption markers in gingival crevicular fluid of rheumatoid arthritis patients with and without periodontitis: a pilot study.” 2012. Web. 17 Apr 2021.

Vancouver:

Kobayashi K. Expression of bone resorption markers in gingival crevicular fluid of rheumatoid arthritis patients with and without periodontitis: a pilot study. [Internet] [Thesis]. University of Adelaide; 2012. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/2440/77344.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kobayashi K. Expression of bone resorption markers in gingival crevicular fluid of rheumatoid arthritis patients with and without periodontitis: a pilot study. [Thesis]. University of Adelaide; 2012. Available from: http://hdl.handle.net/2440/77344

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Chicago

7. Lazari, Paul. Secretory Micro-RNA 29 in Gingival Crevicular Fluid During Canine Retraction.

Degree: 2016, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/20907

► Introduction: Gingival crevicular fluid (GCF) has been widely investigated as a potential source of biomarkers for an individual’s oral and general health information. MicroRNAs (miRNAs)… (more)

▼ Introduction: Gingival crevicular fluid (GCF) has been widely investigated as a potential source of biomarkers for an individual’s oral and general health information. MicroRNAs (miRNAs) are non-coding RNAs that are involved in post-transcriptional gene regulation. In this study, we investigated potentials of certain miRNAs in GCF as biomarkers for detection of periodontal remodeling during OTM. The aim of the study was to confirm the presence of secretory miRNAs in GCF and investigate temporal expression profiles of specific secretory miRNAs during the course of orthodontic tooth movement. Methods: A total of 70 subjects were recruited in the study from the patients receiving treatment at the University of Illinois at Chicago’s orthodontic clinic. The inclusion criteria included subjects requiring extraction of maxillary first premolars as part of their comprehensive treatment with edgewise fixed appliances. Ultimately 11 GCF samples were collected from healthy subjects between the ages 10 and 18 (mean 14.5 years old), who maintained excellent oral hygiene throughout the study. GCF was collected using absorbent Periopaper strip at six timepoints. T0: prior to bonding the fixed orthodontic appliances T1: on the day of canine retraction T2: 60 minutes after activating the power chain T3: 1 day after the canine retraction visit T4: 7 days after the canine retraction visit T5: between 5 weeks after initiation of canine retraction Human Let7d, g and i were used for normalization of secretory miRNA levels in GCF. Wilcoxon Sign Rank and Kruskal-Wallis and Mann-Whitney U statistical analyses were used in this study Results: In all studied miRNAs, the change in expression of miRNA from T1 to T5 showed statistical significance consistently (p-value ranging from 0.005 to 0.047). Changes between T1 and T2 were only observed in miRNA-29b. Changes between T1 and T4 were observed in both miRNA-101 and miRNA-29b. Analyzing all the time points together, Kruskal-Wallis test showed no statistical significant difference between miRNA-29a, miRNA-29b and miRNA-29c, (p>0.05, p-value ranging from 0.201 to 0.802) indicating the similarity in profile change of miRNA-29 family. Conclusions: We concluded that secretory miRNAs exist in GCF and the expression levels of miRNA-29 family change during orthodontic tooth movement. Advisors/Committee Members: Atsawasuwan, Phimon (advisor), Evans, Carla (committee member), Viana, Maria Grace (committee member).

Subjects/Keywords: MicroRNA; MiRNA; GCF; orthodontics; orthodontic tooth movement; gingival crevicular fluid; biomarkers

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lazari, P. (2016). Secretory Micro-RNA 29 in Gingival Crevicular Fluid During Canine Retraction. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/20907

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lazari, Paul. “Secretory Micro-RNA 29 in Gingival Crevicular Fluid During Canine Retraction.” 2016. Thesis, University of Illinois – Chicago. Accessed April 17, 2021. http://hdl.handle.net/10027/20907.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lazari, Paul. “Secretory Micro-RNA 29 in Gingival Crevicular Fluid During Canine Retraction.” 2016. Web. 17 Apr 2021.

Vancouver:

Lazari P. Secretory Micro-RNA 29 in Gingival Crevicular Fluid During Canine Retraction. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/10027/20907.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lazari P. Secretory Micro-RNA 29 in Gingival Crevicular Fluid During Canine Retraction. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/20907

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

8. Mcneight, Angela Marie. The Effect of Vibratory Stimulation and Tooth Movement on Gingival Crevicular Fluid Biomarkers.

Degree: MS, Dental Sciences - Dentistry, 2016, University of Florida

URL: https://ufdc.ufl.edu/UFE0050044

► In the field of orthodontics, gingival crevicular fluid (GCF) is one of the main targets for assessing biomarkers. The term GCF describes the fluid that… (more)

▼ In the field of orthodontics, gingival crevicular fluid (GCF) is one of the main targets for assessing biomarkers. The term GCF describes the fluid that escapes from between the tooth surface and the epithelial lining of the periodontal ligament (PDL). There are over 100 regulatory proteins that can be detected in GCF, and they can be categorized on the basis of their biological significance such as bone resorption or formation. OBJECTIVES: To investigate the effect of vibratory stimulation (AcceleDent(R) Aura) on the expression of biomarkers in GCF using a human tooth movement model with clear aligners. METHODS: Prospective, single-center, randomized controlled crossover study comparing an active vibration device to a sham device. Participants received 6 Zendura(R) plastic aligners to move a target tooth 1.98 mm over 12 weeks. Patients were crossed over to the opposite device at the 4th aligner. GCF was collected at T0 (baseline 1), T1 (baseline 2), T2 (week 1), T3 (week 7, crossover) and T4 (week 12). 11 biomarker levels quantified by multiplex bead immunoassay. A mixed model analysis was used to compare the effects of active versus sham device on GCF composition. RESULTS: 16 males and 23 females completed the protocol. No statistically significant differences were found between the biomarker levels of the Sham-Active (SA Group) and Active-Sham (AS Group). The results of each group were then combined and biomarker concentrations were compared to tooth movement. There was a negative correlation between the baseline level of IL-6 and the total amount of tooth movement. A negative correlation was seen between the baseline levels of MMP-9 and tooth movement between weeks 1-2 followed by a positive correlation between the concentration of MMP-9 at T4 and the amount of tooth movement over the course of the study. CONCLUSIONS: The results suggest that vibration does not influence the secretion of GCF biomarkers; nevertheless, tooth movement with clear aligners seems to alter the proteomic composition of GCF. This study was registered on https://clinicaltrials.gov (ClinicalTrials.gov Identifier: NCT01962012). It was approved by the Western Institutional Review Board (WIRB20131856). This study was funded by OrthoAccel(R) Technologies. ( en ) Advisors/Committee Members: RODY,WELLINGTON JOSE,JR (committee chair), MCGORRAY,SUSAN P (committee member), WALLET,SHANNON MARGARET (committee member).

Subjects/Keywords: Biological markers; Bones; Gingival crevicular fluid; Orthodontics; Orthods; Osteoclasts; Rats; Statistical median; Tooth movement; Vibration; acceledent – crevicular – fluid – gcf – gingival – movement – orthodontics – tooth – vibration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mcneight, A. M. (2016). The Effect of Vibratory Stimulation and Tooth Movement on Gingival Crevicular Fluid Biomarkers. (Masters Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0050044

Chicago Manual of Style (16^th Edition):

Mcneight, Angela Marie. “The Effect of Vibratory Stimulation and Tooth Movement on Gingival Crevicular Fluid Biomarkers.” 2016. Masters Thesis, University of Florida. Accessed April 17, 2021. https://ufdc.ufl.edu/UFE0050044.

MLA Handbook (7^th Edition):

Mcneight, Angela Marie. “The Effect of Vibratory Stimulation and Tooth Movement on Gingival Crevicular Fluid Biomarkers.” 2016. Web. 17 Apr 2021.

Vancouver:

Mcneight AM. The Effect of Vibratory Stimulation and Tooth Movement on Gingival Crevicular Fluid Biomarkers. [Internet] [Masters thesis]. University of Florida; 2016. [cited 2021 Apr 17]. Available from: https://ufdc.ufl.edu/UFE0050044.

Council of Science Editors:

Mcneight AM. The Effect of Vibratory Stimulation and Tooth Movement on Gingival Crevicular Fluid Biomarkers. [Masters Thesis]. University of Florida; 2016. Available from: https://ufdc.ufl.edu/UFE0050044

Freie Universität Berlin

9. Meißner, Simon. Pilot study on the effect of Simvastatin on concentration of Interleukin-6, Interleukin-8, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in gingival crevicular fluid in chronic periodontitis.

Degree: 2013, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/13193

► Interleukins (IL) IL-6 and IL-8 which are involved in acute and chronic inflammation in chronic periodontitis are increased in the gingival crevicular fluid (GCF). These… (more)

▼ Interleukins (IL) IL-6 and IL-8 which are involved in acute and chronic inflammation in chronic periodontitis are increased in the gingival crevicular fluid (GCF). These cyto-kines and inflammatory mediators induce the secretion of Matrix Metalloproteinases (MMP-9) which have a degrading effect on the extracellular tissue and the surrounding bone. Simvastatin is a HMG-CoA reductase inhibitor in treating disorders of lipid metabolism and in preventing cardiovascular disease in high-risk groups. Retrospective studies and clinical in vitro and in vivo (animal) studies have demonstrated a protective effect against progression of periodontal disease, a reduction in bone breakdown and an increase of VEGF in bone resulting in increased bone apposition. The aim of this study was to demonstrate an antiinflammatory effect of simvastatin by standard therapy on chronic periodontitis. Methods 24 patients with chronic periodontitis were included in the study. 12 of whom were treated with simvastatin (treatment group) for at least 3 months, the remaining 12 patients served as controls. None of the patients received immunmodulating therapy. Patients (treatment group / controls) were matched for age, gender and periodontal status. Seven days after the periodontal status was examined and professional tooth cleaning on the first molars of each quadrant was performed, GCF samples were taken twice and quantified. Analysis of GCF was performed after homogenization using commercial sandwich enzyme immunoassays. Measures were performed twice, for statistical analysis calculated medians were used. Two samples of a healthy person served as controls. Results In 24 patients of the treatment group and controls the median age was 66 years. Medi-an duration with regard to periodontitis was 5,5 years for statin-treated patients and 7,7 years for patients without simvastatin-therapy. Both groups did not differ in gender dis-tribution, age, height, weight and periodontal treatment. Median concentrations in GCF for IL-6 and IL-8 for patients taking simvastatin were 3,08 ng / ml (Q25: 2,13 / Q75: 3,92) and 121,74 ng / ml (Q25: 94,06 / Q75: 190,82). Concentrations in patients without statin therapy were at 2,06 ng / ml (Q25: 1,84 / Q75: 3,14) for IL-6 and 132,43 ng / ml (Q25: 84,05 / Q75: 378,42) for IL-8. In the group receiving simvastatin MMP-9 amounted to a median of 27,61 ug / ml (Q25: 12,08 / Q75: 48,15), in the group without statins 28.82 g / ml (Q25: 19,43 / Q75: 46,08). Median concentrations of VEGF in the simvastatin-group were 9,91 ng / ml (Q25: 0 / Q75: 23,18), in the control group 18,36 ng / ml (Q25: 6, 25 / Q75: 22,30). In healthy controls, median IL-6 concentrations were found to be 0,41 ng / ml, in IL-8 30,5 ng / ml, in VEGF 5,40 ng / ml (Q25: 5,24 / Q50 : 5,40), in MMP-9 6,70 ug / ml (Q25: 5,89 / Q50: 6,70). Statin-treated patients did not show significant differences neither in IL-6 and IL-8 concentrations, nor in VEGF and MMP-9. Significant differences were observed regarding healthy control. No correlations regarding disease duration,… Advisors/Committee Members: [email protected] (contact), m (gender), N.N. (firstReferee), N.N. (furtherReferee).

Subjects/Keywords: chronic periodontitis; Simvastatin; GCF; IL-6; IL-8; VEGF; MMP-9; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Meißner, S. (2013). Pilot study on the effect of Simvastatin on concentration of Interleukin-6, Interleukin-8, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in gingival crevicular fluid in chronic periodontitis. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Meißner, Simon. “Pilot study on the effect of Simvastatin on concentration of Interleukin-6, Interleukin-8, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in gingival crevicular fluid in chronic periodontitis.” 2013. Thesis, Freie Universität Berlin. Accessed April 17, 2021. https://refubium.fu-berlin.de/handle/fub188/13193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Meißner, Simon. “Pilot study on the effect of Simvastatin on concentration of Interleukin-6, Interleukin-8, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in gingival crevicular fluid in chronic periodontitis.” 2013. Web. 17 Apr 2021.

Vancouver:

Meißner S. Pilot study on the effect of Simvastatin on concentration of Interleukin-6, Interleukin-8, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in gingival crevicular fluid in chronic periodontitis. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2021 Apr 17]. Available from: https://refubium.fu-berlin.de/handle/fub188/13193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meißner S. Pilot study on the effect of Simvastatin on concentration of Interleukin-6, Interleukin-8, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in gingival crevicular fluid in chronic periodontitis. [Thesis]. Freie Universität Berlin; 2013. Available from: https://refubium.fu-berlin.de/handle/fub188/13193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

10. Saidowa, Asisa. correlation coefficients between a modified Papilla Bleeding Index and the amount of crevicular fluid in patients suffering from generalized aggressive periodontitis.

Degree: 2011, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/11142

► The periodontitis is an inflammatory disease condition caused by a variety of oral microorganisms. A hyper reactive host response regulation is triggering the disease progression… (more)

▼ The periodontitis is an inflammatory disease condition caused by a variety of oral microorganisms. A hyper reactive host response regulation is triggering the disease progression in a small part of the world wide population – around 10% at most – resulting either in a local or generalized aggressive form of manifestation. Bleeding is a final act in a process of tissue and capillary damage initiated by bacterial presence and modulated by pro-inflammatory cytokines. Calibrated assignment of bleeding intensity as a parameter for the severity of the disease is considered golden standard for diagnosing gingivitis – an inflammatory condition restricted to the soft tissues. Periodontal inflammation regularly causes attachment loss and is accompanied by loss of alveolar bone and a phenomenon named “pocketing”. The severity of this disease condition is currently characterized by assessing either the probing pocket depths (PPD) or the attachment loss (AL). The evaluation of bleeding tendency as Bleeding on Probing (BoP) is currently an additional dichotomous finding. The aim of the present study was to assess the suitability of a visual calibration of a Pocket Bleeding Index (PoB-Index) to reflect the real inflammatory status of each single probed periodontal pocket. By matching single grades of the index suggested – i.e. the single visually assigned bleeding grades - with parameters such as bleeding duration (stop watch) and amount of blood sampled by capillary tube correlation coefficients were calculated. Correlations between PoB-Index and the probing depth and PoBI and the amount of gingival crevicular fluid (GCF) sampled as exudates that follow a gradient were calculated also to objectify the visual calibration of bleeding intensity. The capillary dilatation is initiated as first step in a cascade of inflammatory reactions in front of all clinical signs and results in a measurable increase of the GCF. Since periodontitis progresses episodically, i.e. phases of activity are followed by phases of inactivity the capillary dilatation follows the same pattern. The mean age of 17 participating patients recruited was 32 years; 10 of them were non-smokers whereas 7 were smoking. All patients enrolled did not have any periodontitis therapy or received any antibiotics prescribed for the last 12 months. All patients underwent intensive instructions in oral hygiene repeatedly, thus the allocated plaque index remained for the duration of the study < 25% (API < 25%). After completion of instrumentation (S/RP) all patients received Amoxicillin (500 mg) and Metronidazol (250 mg) x 3 daily for 10 days per os prescribed. The assessment of the study parameters was carried out at baseline after completion of the initial therapy (before S/RP), at month 3, 6 and 9 past active therapy. In total 575 sites were evaluated and monitored. For improving the reproducibility, all measurements were undertaken under equal conditions in all patients. The statistically significant correlation between the GCF amount and the PoB-Index grades at baseline… Advisors/Committee Members: [email protected] (contact), w (gender), Prof. Dr. A. Friedmann (firstReferee), Prof. Dr. T. Kocher (furtherReferee).

Subjects/Keywords: generalized aggressive periodontitis; Bleeding on Probing (BOP); Pocket Bleeding Index (PoBI); gingival crevicular fluid (GCF); 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saidowa, A. (2011). correlation coefficients between a modified Papilla Bleeding Index and the amount of crevicular fluid in patients suffering from generalized aggressive periodontitis. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/11142

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Saidowa, Asisa. “correlation coefficients between a modified Papilla Bleeding Index and the amount of crevicular fluid in patients suffering from generalized aggressive periodontitis.” 2011. Thesis, Freie Universität Berlin. Accessed April 17, 2021. https://refubium.fu-berlin.de/handle/fub188/11142.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Saidowa, Asisa. “correlation coefficients between a modified Papilla Bleeding Index and the amount of crevicular fluid in patients suffering from generalized aggressive periodontitis.” 2011. Web. 17 Apr 2021.

Vancouver:

Saidowa A. correlation coefficients between a modified Papilla Bleeding Index and the amount of crevicular fluid in patients suffering from generalized aggressive periodontitis. [Internet] [Thesis]. Freie Universität Berlin; 2011. [cited 2021 Apr 17]. Available from: https://refubium.fu-berlin.de/handle/fub188/11142.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Saidowa A. correlation coefficients between a modified Papilla Bleeding Index and the amount of crevicular fluid in patients suffering from generalized aggressive periodontitis. [Thesis]. Freie Universität Berlin; 2011. Available from: https://refubium.fu-berlin.de/handle/fub188/11142

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Vollmar, Christine. Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?.

Degree: MS, Dentistry, 2015, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1435011386

► Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?, Vollmar C, McTigue D, Kumar P, Subramanian K, Kumar A, (Nationwide Children’s Hospital and… (more)

▼ Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?, Vollmar C, McTigue D, Kumar P, Subramanian K, Kumar A, (Nationwide Children’s Hospital and The Ohio State University, Columbus, OH)Purpose: Determine if gingival crevicular fluid transcriptome is altered after dental trauma and if these changes persist over time.Methods: Gingival crevicular fluid (GCF) samples were obtained from patients following permanent anterior tooth dental trauma who presented to Nationwide Children’s Hospital Emergency Department or Emergency Dental Clinic. Samples were obtained from the gingival sulcus using PerioPaper strip, placed in a sterile 10ml vial containing RNAlater, and stored at minus twenty degrees Fahrenheit. GCF sampling was repeated at routine follow-up encounters at 2-4 weeks, 6 weeks, 2 months, and 4-6 months when possible. Samples that were analyzed were divided into one of four categories of trauma: subluxation, uncomplicated crown fracture, extrusion, and avulsion. Four patients were selected from each group that demonstrated the most homogenous characteristics for sequencing and data analysis. RNA was isolated using epicenter baseline-ZERO DNase and Ribo-Zero Magnetic Kit. mRNA was enriched using ice-cold ethanol. cDNA conversion was performed using the Scriptseq kit from the stabilized mRNA. Pooled cDNA libraries were clustered on the HiSeq and 250bp paired-end sequencing was performed. The filtered sequences were uploaded to the Kallisto program, which was used to align and quantify abundances of transcripts from the filtered sequences. Sequences were aligned to GRCh38 and estimated gene counts were obtained and uploaded in PANTHER (Protein Annotation Through Evolutionary Relationships) gene analysis platform for analysis. Statistical over-representation test with Bonferroni correction was carried out to obtain the difference in fold enrichment of genes and functions between the groups. Significantly different pathways were visualized using PANTHER mapping systems.Results: At the initial encounter, the avulsion group shows the least number of gene transcript families present, whereas the uncomplicated crown fracture and extrusion groups had the most. By the final encounter, the avulsion group had the most transcript families expressed, while the crown fracture and extrusion groups showed the least. The subluxation group had an intermediate number of transcript families present immediately after dental trauma that slightly increased at the final visit. The quantity and quality of gene expression varied between groups at both the initial and final encounter. Looking at the PANTHER pathway maps, specific pathways were up regulated in different groups.Conclusion: GCF Transcriptome displays changes in gene expression immediately after dental trauma representing a rapid response to injury at the level of the genome. The transcriptome remains elevated overtime in all groups, however, some types of trauma show more of a prolonged response while others return closer to baseline. Advisors/Committee Members: McTigue, Dennis (Advisor).

Subjects/Keywords: Dentistry; Dental Care; dental trauma; dental injury; GCF; human transcriptomics

…16 Figure 2: GCF Transcriptome immediately following dental trauma �� …...18 Figure 3… …GCF Transcriptome at final encounter �� …...19 Figure 4: Inflammation mediated by… …Gingival crevicular fluid (GCF) is a biological substance secreted within the gingival… …studies of periodontal disease and orthodontic root resorption 20-22. GCF is readily available… …provide valuable insight into healing after dental trauma. Previous studies of the GCF after…

13 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vollmar, C. (2015). Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1435011386

Chicago Manual of Style (16^th Edition):

Vollmar, Christine. “Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?.” 2015. Masters Thesis, The Ohio State University. Accessed April 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1435011386.

MLA Handbook (7^th Edition):

Vollmar, Christine. “Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?.” 2015. Web. 17 Apr 2021.

Vancouver:

Vollmar C. Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?. [Internet] [Masters thesis]. The Ohio State University; 2015. [cited 2021 Apr 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1435011386.

Council of Science Editors:

Vollmar C. Can the Gingival Crevicular Fluid Transcriptome Predict Healing After Dental Trauma?. [Masters Thesis]. The Ohio State University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1435011386

University of Florida

12. Wahl, Kelsey Cronauer. Micrornas as Biomarkers in Gingival Crevicular Fluid.

Degree: MS, Dental Sciences - Dentistry, 2020, University of Florida

URL: https://ufdc.ufl.edu/UFE0056549

► In medicine and dentistry there is a significant focus on improving patient diagnosis and outcomes. With the identification of exosomes as methods of intercellular communication… (more)

▼ In medicine and dentistry there is a significant focus on improving patient diagnosis and outcomes. With the identification of exosomes as methods of intercellular communication and genetic exchange, another avenue for biomarker discovery presented itself. Released from osteoclasts and inflammatory cells, extracellular vesicles carry genetic information, such as microRNAs (miRNAs), which can alter gene transcription in target cells and affect the inflammatory and bone remodeling processes. Gingival crevicular fluid (GCF) is a commonly used fluid for detection of biomarkers, including miRNAs. The objective of this study was to determine if miRNA146a could be sufficiently collected by GCF methods and which method was best for collecting this miRNA. This study compared three different methods which are the following: Periopaper strips, Durapore filter membrane, and micropipettes. Twelve adult volunteers were sampled and sampling took place over two visits. For each subject three teeth were sampled, each tooth with one of the proposed methods. A pattern was established so that collection method varied per tooth but repeated every fourth subject, for comparison. Collections were taken on the mesial and distal surfaces of each specified tooth. RNA isolation, cDNA synthesis, and real time polymerase chain reaction (qPCR) were performed on each sample to assess expression of miRNA146a. A reference gene, miRNA103,3p, was also used for assessment and normalization of data. Samples from seven subjects were analyzed and the results showed that there was no significant difference in method of collection for identifying and expressing miRNA146a. However, a secondary analysis of samples showed that there is a significant difference in expression of miRNA146a between subjects who were undergoing orthodontic treatment and those who were untreated. This study concluded that there is no statistical advantage to using one of the three collection methods over another for expression of miRNA146a. ( en ) Advisors/Committee Members: Holliday,Lexie Shannon (committee chair), Caudle,Robert M (committee member).

Subjects/Keywords: biomarker – gcf – microrna – orthodontic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wahl, K. C. (2020). Micrornas as Biomarkers in Gingival Crevicular Fluid. (Masters Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0056549

Chicago Manual of Style (16^th Edition):

Wahl, Kelsey Cronauer. “Micrornas as Biomarkers in Gingival Crevicular Fluid.” 2020. Masters Thesis, University of Florida. Accessed April 17, 2021. https://ufdc.ufl.edu/UFE0056549.

MLA Handbook (7^th Edition):

Wahl, Kelsey Cronauer. “Micrornas as Biomarkers in Gingival Crevicular Fluid.” 2020. Web. 17 Apr 2021.

Vancouver:

Wahl KC. Micrornas as Biomarkers in Gingival Crevicular Fluid. [Internet] [Masters thesis]. University of Florida; 2020. [cited 2021 Apr 17]. Available from: https://ufdc.ufl.edu/UFE0056549.

Council of Science Editors:

Wahl KC. Micrornas as Biomarkers in Gingival Crevicular Fluid. [Masters Thesis]. University of Florida; 2020. Available from: https://ufdc.ufl.edu/UFE0056549

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Open Access Theses and Dissertations