University of Florida
Wahl, Kelsey Cronauer.
Micrornas as Biomarkers in Gingival Crevicular Fluid.
Degree: MS, Dental Sciences - Dentistry, 2020, University of Florida
In medicine and dentistry there is a significant focus on improving patient diagnosis and outcomes. With the identification of exosomes as methods of intercellular communication and genetic exchange, another avenue for biomarker discovery presented itself. Released from osteoclasts and inflammatory cells, extracellular vesicles carry genetic information, such as microRNAs (miRNAs), which can alter gene transcription in target cells and affect the inflammatory and bone remodeling processes. Gingival crevicular fluid (GCF) is a commonly used fluid for detection of biomarkers, including miRNAs. The objective of this study was to determine if miRNA146a could be sufficiently collected by GCF methods and which method was best for collecting this miRNA. This study compared three different methods which are the following: Periopaper strips, Durapore filter membrane, and micropipettes. Twelve adult volunteers were sampled and sampling took place over two visits. For each subject three teeth were sampled, each tooth with one of the proposed methods. A pattern was established so that collection method varied per tooth but repeated every fourth subject, for comparison. Collections were taken on the mesial and distal surfaces of each specified tooth. RNA isolation, cDNA synthesis, and real time polymerase chain reaction (qPCR) were performed on each sample to assess expression of miRNA146a. A reference gene, miRNA103,3p, was also used for assessment and normalization of data. Samples from seven subjects were analyzed and the results showed that there was no significant difference in method of collection for identifying and expressing miRNA146a. However, a secondary analysis of samples showed that there is a significant difference in expression of miRNA146a between subjects who were undergoing orthodontic treatment and those who were untreated. This study concluded that there is no statistical advantage to using one of the three collection methods over another for expression of miRNA146a. ( en )
Advisors/Committee Members: Holliday,Lexie Shannon (committee chair), Caudle,Robert M (committee member).
Subjects/Keywords: biomarker – gcf – microrna – orthodontic
to Zotero / EndNote / Reference
APA (6th Edition):
Wahl, K. C. (2020). Micrornas as Biomarkers in Gingival Crevicular Fluid. (Masters Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0056549
Chicago Manual of Style (16th Edition):
Wahl, Kelsey Cronauer. “Micrornas as Biomarkers in Gingival Crevicular Fluid.” 2020. Masters Thesis, University of Florida. Accessed April 15, 2021.
MLA Handbook (7th Edition):
Wahl, Kelsey Cronauer. “Micrornas as Biomarkers in Gingival Crevicular Fluid.” 2020. Web. 15 Apr 2021.
Wahl KC. Micrornas as Biomarkers in Gingival Crevicular Fluid. [Internet] [Masters thesis]. University of Florida; 2020. [cited 2021 Apr 15].
Available from: https://ufdc.ufl.edu/UFE0056549.
Council of Science Editors:
Wahl KC. Micrornas as Biomarkers in Gingival Crevicular Fluid. [Masters Thesis]. University of Florida; 2020. Available from: https://ufdc.ufl.edu/UFE0056549