subject:(Fungal)

Universiteit Utrecht

1. Gruntjes, T. Molecular mechanisms behind fungal-fungal and fungal-bacterial interactions.

Degree: 2013, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/287992

► Research on fungi has mostly focused on monocultures, however, fungi are almost always interacting with other fungi and bacteria in natural circumstances. Many such interactions… (more)

Subjects/Keywords: Fungal-fungal interactions; Fungal-bacterial interactions.

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APA (6^th Edition):

Gruntjes, T. (2013). Molecular mechanisms behind fungal-fungal and fungal-bacterial interactions. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/287992

Chicago Manual of Style (16^th Edition):

Gruntjes, T. “Molecular mechanisms behind fungal-fungal and fungal-bacterial interactions.” 2013. Masters Thesis, Universiteit Utrecht. Accessed January 23, 2021. http://dspace.library.uu.nl:8080/handle/1874/287992.

MLA Handbook (7^th Edition):

Gruntjes, T. “Molecular mechanisms behind fungal-fungal and fungal-bacterial interactions.” 2013. Web. 23 Jan 2021.

Vancouver:

Gruntjes T. Molecular mechanisms behind fungal-fungal and fungal-bacterial interactions. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Jan 23]. Available from: http://dspace.library.uu.nl:8080/handle/1874/287992.

Council of Science Editors:

Gruntjes T. Molecular mechanisms behind fungal-fungal and fungal-bacterial interactions. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/287992

University of Sydney

2. LIU, Qi. The link between Fungal nutrition and Fungal Phenotype .

Degree: 2014, University of Sydney

URL: http://hdl.handle.net/2123/12035

► Carbon and nitrogen are key macro-nutrients affecting growth and fitness particularly in heterotrophic organisms. A considerable body of evidence suggests that many organisms including mammals,… (more)

▼ Carbon and nitrogen are key macro-nutrients affecting growth and fitness particularly in heterotrophic organisms. A considerable body of evidence suggests that many organisms including mammals, insects and slime moulds have a target intake that is optimal in the sense of maximising growth or ecological fitness. In nutritionally heterogeneous environments, these organisms show an ability to regulate the intake of carbon (C) and nitrogen (N) compounds by selecting different food types to reach the target ratio. This is now a major focus of research in diet-related chronic disease in humans including obesity. Fungi are one of the most important components of the terrestrial ecosystem, and play a key role in nutrient cycling, structural genesis, water infiltration and carbon storage in soil. The manner in which the fungal phenotype emerges in response to the complex nutritional environment of soil is fundamental to the persistence of these functions across space and time. We explore the extent to which the fungal phenotype can be understood in terms of a target ratio of C: N. We used the fungus Mucor mucedo as the model species, and studies its growth in different nutrient regimes by varying the C: N ratio, and including both organic and inorganic sources of nitrogen. There is evidence for a target C: N ratio in a homogeneous environment, although growth rate remains high over a relatively broad range in the ratio by comparison with other organisms. We attribute this to the capacity of fungi to recycle and translocate internal sources of nutrients to regions of high demand. In a heterogeneous environment, we provide evidence that this is the case, although nitrogen is more readily translocated than carbon in this species. In this study, a comparison of growth rate for different C: N ratios and nutrient concentrations indicates efficiency, the amplitude of the oscillations is a measure of stability. This provides an important constraint for our understanding of underlying regulatory pathways linking C and N to growth. A hyphal-level II model for fungal growth is developed to study the consequences of our findings for the emergence of the fungal phenotype, and is used to generate new hypotheses for future testing. Finally, a metabolic model is constructed that synthesises existing knowledge of carbon and nitrogen pathways in cells. We built two versions of this network model corresponding to the case of organic and inorganic sources of nitrogen respectively. Both models reproduced oscillatory growth observed in the laboratory experiments and, consistent with observation, the amplitude of the oscillations is positively correlated with the C: N ratio only for the inorganic N version of the model. A peak C: N ratio for fungal growth is also only predicted for inorganic sources of N, as we saw in the observed behaviour. The networks we built for this project may be highly conserved across the kingdom of life, therefore the models may be broadly applicable with certain modifications.

Subjects/Keywords: Fungal nutrition; Fungal Phenotype

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APA (6^th Edition):

LIU, Q. (2014). The link between Fungal nutrition and Fungal Phenotype . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/12035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

LIU, Qi. “The link between Fungal nutrition and Fungal Phenotype .” 2014. Thesis, University of Sydney. Accessed January 23, 2021. http://hdl.handle.net/2123/12035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

LIU, Qi. “The link between Fungal nutrition and Fungal Phenotype .” 2014. Web. 23 Jan 2021.

Vancouver:

LIU Q. The link between Fungal nutrition and Fungal Phenotype . [Internet] [Thesis]. University of Sydney; 2014. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2123/12035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIU Q. The link between Fungal nutrition and Fungal Phenotype . [Thesis]. University of Sydney; 2014. Available from: http://hdl.handle.net/2123/12035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Hopkins, Steven Michael. A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition.

Degree: PhD, University of Glamorgan, 2012, University of South Wales

URL: http://hdl.handle.net/10265/574

► Fungi are a crucial component of most ecosystems and are responsible for decomposing organic matter, distributing nutrients through the environment and supporting plants and animal… (more)

Subjects/Keywords: Fungal mycelia

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APA (6^th Edition):

Hopkins, S. M. (2012). A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition. (Doctoral Dissertation). University of South Wales. Retrieved from http://hdl.handle.net/10265/574

Chicago Manual of Style (16^th Edition):

Hopkins, Steven Michael. “A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition.” 2012. Doctoral Dissertation, University of South Wales. Accessed January 23, 2021. http://hdl.handle.net/10265/574.

MLA Handbook (7^th Edition):

Hopkins, Steven Michael. “A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition.” 2012. Web. 23 Jan 2021.

Vancouver:

Hopkins SM. A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition. [Internet] [Doctoral dissertation]. University of South Wales; 2012. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10265/574.

Council of Science Editors:

Hopkins SM. A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition. [Doctoral Dissertation]. University of South Wales; 2012. Available from: http://hdl.handle.net/10265/574

4. Issi, Luca. Identification and characterization of novel putative virulence factors in Candida albicans.

Degree: PhD, 2014, Worcester Polytechnic Institute

URL: etd-091814-073712 ; https://digitalcommons.wpi.edu/etd-dissertations/385

► "The C. albicans community is currently laying the foundation of understanding how this human pathogen causes infection. C. albicans infections represent a major medical and… (more)

▼ "The C. albicans community is currently laying the foundation of understanding how this human pathogen causes infection. C. albicans infections represent a major medical and economic burden for today’s society with an estimated 400,000 blood stream infections worldwide and direct costs exceeding 1$ billion dollar a year in the U.S. alone. Although finding the biological causes of this disease seemed to be beyond our reach in the past, various aspects of the infection have been recently unveiled including its pathology, immunology, histology, and epidemiology. Here we explored the genetic components of this disease by studying the complex host-pathogen dynamics through a series of in vivo, ex vivo and in vitro experiments. By using a pathogen unbiased reverse genetic approach and a host gene candidate strategy we uncovered some of the genes and pathways that are important for pathogenicity and immunity. In particular we explored the complex host-pathogen dynamics using a C. albicans - C. elegans model system and identified four novel putative virulence factors. We focused on Zcf15, a C. albicans transcription factor that has been poorly characterized in the literature and that plays an important role in the pathogen’s ability to resist host generated reactive oxygen species (ROS). By leveraging the power of RNASeq and ChIP-Seq we identified Zcf15 transcriptional targets and DNA binding sites. These studies suggest that Zcf15 plays a critical role in carbon metabolism and that it exerts its ability to protect the pathogen from ROS by controlling the expression of thiol- peroxidases and other detoxifying enzymes. We also showed here that in C. elegans, the host’s ability to counteract the infection relies on the MAPK pathway, evidence that mirrors what has been found by others in mammals and that emphasizes the usefulness of studying C. albicans infections in smaller genetically traceable organisms like C. elegans. The nematode model is also shown here to be a powerful tool not only to study the genetic bases that drive infection and immunity but also to identify new compounds that can be used for therapeutic intervention. This model was instrumental in identifying filastatin, a small molecule that was subsequently found by our collaborators to be capable of reducing virulence in mammals. The antifungal properties of filastatin are currently undertaking further preclinical testing. Overall this thesis shed light on the complex mechanisms of C. albicans pathogenicity and host immunity and identified novel virulence determinants that can be used by the larger community for further biological studies or even drug development. " Advisors/Committee Members: Reeta Rao, Advisor, Elizabeth F. Ryder, Committee Member, Luis Vidali, Committee Member, Dawn Thompson, Committee Member, Sanjay Jain, Committee Member.

Subjects/Keywords: Fungal infections

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APA (6^th Edition):

Issi, L. (2014). Identification and characterization of novel putative virulence factors in Candida albicans. (Doctoral Dissertation). Worcester Polytechnic Institute. Retrieved from etd-091814-073712 ; https://digitalcommons.wpi.edu/etd-dissertations/385

Chicago Manual of Style (16^th Edition):

Issi, Luca. “Identification and characterization of novel putative virulence factors in Candida albicans.” 2014. Doctoral Dissertation, Worcester Polytechnic Institute. Accessed January 23, 2021. etd-091814-073712 ; https://digitalcommons.wpi.edu/etd-dissertations/385.

MLA Handbook (7^th Edition):

Issi, Luca. “Identification and characterization of novel putative virulence factors in Candida albicans.” 2014. Web. 23 Jan 2021.

Vancouver:

Issi L. Identification and characterization of novel putative virulence factors in Candida albicans. [Internet] [Doctoral dissertation]. Worcester Polytechnic Institute; 2014. [cited 2021 Jan 23]. Available from: etd-091814-073712 ; https://digitalcommons.wpi.edu/etd-dissertations/385.

Council of Science Editors:

Issi L. Identification and characterization of novel putative virulence factors in Candida albicans. [Doctoral Dissertation]. Worcester Polytechnic Institute; 2014. Available from: etd-091814-073712 ; https://digitalcommons.wpi.edu/etd-dissertations/385

5. Hopkins, Steven Michael. A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition.

Degree: PhD, 2011, University of South Wales

URL: https://pure.southwales.ac.uk/en/studentthesis/a-hybrid-mathematical-model-of-fungal-mycelia(e576adeb-72bb-4848-a1dd-e614075c485c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543222

► Fungi are a crucial component of most ecosystems and are responsible for decomposing organic matter, distributing nutrients through the environment and supporting plants and animal… (more)

Subjects/Keywords: 579.5; Fungal mycelia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hopkins, S. M. (2011). A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition. (Doctoral Dissertation). University of South Wales. Retrieved from https://pure.southwales.ac.uk/en/studentthesis/a-hybrid-mathematical-model-of-fungal-mycelia(e576adeb-72bb-4848-a1dd-e614075c485c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543222

Chicago Manual of Style (16^th Edition):

Hopkins, Steven Michael. “A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition.” 2011. Doctoral Dissertation, University of South Wales. Accessed January 23, 2021. https://pure.southwales.ac.uk/en/studentthesis/a-hybrid-mathematical-model-of-fungal-mycelia(e576adeb-72bb-4848-a1dd-e614075c485c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543222.

MLA Handbook (7^th Edition):

Hopkins, Steven Michael. “A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition.” 2011. Web. 23 Jan 2021.

Vancouver:

Hopkins SM. A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition. [Internet] [Doctoral dissertation]. University of South Wales; 2011. [cited 2021 Jan 23]. Available from: https://pure.southwales.ac.uk/en/studentthesis/a-hybrid-mathematical-model-of-fungal-mycelia(e576adeb-72bb-4848-a1dd-e614075c485c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543222.

Council of Science Editors:

Hopkins SM. A hybrid mathematical model of fungal mycelia : tropisms, polarised growth and application to colony competition. [Doctoral Dissertation]. University of South Wales; 2011. Available from: https://pure.southwales.ac.uk/en/studentthesis/a-hybrid-mathematical-model-of-fungal-mycelia(e576adeb-72bb-4848-a1dd-e614075c485c).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543222

University of Illinois – Urbana-Champaign

6. Pohly, Andrea E. Pathological characterization of natural Ophidiomyces ophiodiicola infection in wild-caught Lake Erie watersnakes: A standardized approach to documentation of disease.

Degree: MS, VMS-Veterinary Clinical Medcne, 2020, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/108509

► Ophidiomyces ophiodiicola (formerly Chrysosporium ophiodiicola), the causative agent of ophidiomycosis (Snake Fungal Disease; SFD), is a serious emerging fungal pathogen causing morbidity and mortality in… (more)

▼ Ophidiomyces ophiodiicola (formerly Chrysosporium ophiodiicola), the causative agent of ophidiomycosis (Snake Fungal Disease; SFD), is a serious emerging fungal pathogen causing morbidity and mortality in both wild and captive snakes. SFD has been documented in terrestrial and aquatic captive snake populations in the United Kingdom, Germany, Australia, and North America. The most common presenting clinical signs of SFD infection include severe dermatitis, facial swelling, and emaciation. It was previously thought that lesions associated with SFD were limited to the skin of the head and neck; however, recent reports show well-documented cases of SFD skin lesions throughout many regions of the body. These case reports predominantly focus on grossly visible cutaneous lesions. Rarely, systemic lesions within the lungs, spleen, liver, eye, and kidneys have been documented. SFD can be fatal; however, the complications of this disease leading to mortality, particularly concerning the pathology and role of comorbidities, are poorly understood. This lack of understanding limits the ability and success of clinicians and rehabilitators to treat snakes infected with SFD and inhibits our ability to prevent transmission in both captive and wild settings. Treatment and preventing transmission are particularly important for those species that are endangered or threatened. The aim of this study was to gain insight into the cutaneous disease severity, subclinical manifestation, systemic involvement, and comorbidities associated with SFD. I hypothesized the following: 1) the systematic mapping protocol derived from this methodology will identify characteristic lesions of SFD in a cohort of wild-caught Lake Erie water snakes, 2) as the number of survey sections affected by SFD increases, so too will the overall cutaneous severity score, 3) histopathological examination will allow for detection of subclinical lesions that are not associated with gross disease, 4) individuals with visceral granulomas will have more severe cutaneous disease, 5) there will be a range of infectious and non-infectious conditions associated with ophidiomycosis, and finally 6) individuals with decreased visceral adipose tissue will have a higher overall cutaneous severity score as well as head cutaneous severity score than those with adequate visceral adipose tissue. This study established a detailed necropsy protocol that identified a positive correlation between cutaneous severity and a wider distribution in cutaneous lesions throughout an individual. The results also highlighted a high prevalence (45.3%) of subclinical, or grossly inapparent, cutaneous lesions as well as a lack of correlation between cutaneous severity and visceral granulomas. Finally, this study documented numerous comoribidities, including verminous pneumonia, heterophilic enteritis and splenitis, cutaneous mites, and coccidiosis. The most-prevalent comorbidity was decreased visceral adipose tissue stores which was not associated with cutaneous disease severity. The results of this… Advisors/Committee Members: Allender, Matthew (advisor), LoBato, N Denae (committee member), Hoyer, Lois (committee member), Terio, Karen (committee member).

Subjects/Keywords: Snake; Fungal; SFD

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APA (6^th Edition):

Pohly, A. E. (2020). Pathological characterization of natural Ophidiomyces ophiodiicola infection in wild-caught Lake Erie watersnakes: A standardized approach to documentation of disease. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/108509

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pohly, Andrea E. “Pathological characterization of natural Ophidiomyces ophiodiicola infection in wild-caught Lake Erie watersnakes: A standardized approach to documentation of disease.” 2020. Thesis, University of Illinois – Urbana-Champaign. Accessed January 23, 2021. http://hdl.handle.net/2142/108509.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pohly, Andrea E. “Pathological characterization of natural Ophidiomyces ophiodiicola infection in wild-caught Lake Erie watersnakes: A standardized approach to documentation of disease.” 2020. Web. 23 Jan 2021.

Vancouver:

Pohly AE. Pathological characterization of natural Ophidiomyces ophiodiicola infection in wild-caught Lake Erie watersnakes: A standardized approach to documentation of disease. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2020. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2142/108509.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pohly AE. Pathological characterization of natural Ophidiomyces ophiodiicola infection in wild-caught Lake Erie watersnakes: A standardized approach to documentation of disease. [Thesis]. University of Illinois – Urbana-Champaign; 2020. Available from: http://hdl.handle.net/2142/108509

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Youngstown State University

7. Price, Eric C. Genotypic and Phenotypic Characterization of Penicillium marneffei Mutants Produced by Agrobacterium-Mediated Transformation.

Degree: MSin Biological Sciences, Department of Biological Sciences and Chemistry, 2012, Youngstown State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1341255805

► The dimorphic fungus Penicillium marneffei is an etiological agent of penicillosis and the third leading cause of AIDS related death in Southeast Asia. This… (more)

Subjects/Keywords: Biology; fungal genomics; fungal dimorphism; agrobacterium-mediated transformation; fungal pathogens

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APA (6^th Edition):

Price, E. C. (2012). Genotypic and Phenotypic Characterization of Penicillium marneffei Mutants Produced by Agrobacterium-Mediated Transformation. (Masters Thesis). Youngstown State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ysu1341255805

Chicago Manual of Style (16^th Edition):

Price, Eric C. “Genotypic and Phenotypic Characterization of Penicillium marneffei Mutants Produced by Agrobacterium-Mediated Transformation.” 2012. Masters Thesis, Youngstown State University. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1341255805.

MLA Handbook (7^th Edition):

Price, Eric C. “Genotypic and Phenotypic Characterization of Penicillium marneffei Mutants Produced by Agrobacterium-Mediated Transformation.” 2012. Web. 23 Jan 2021.

Vancouver:

Price EC. Genotypic and Phenotypic Characterization of Penicillium marneffei Mutants Produced by Agrobacterium-Mediated Transformation. [Internet] [Masters thesis]. Youngstown State University; 2012. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1341255805.

Council of Science Editors:

Price EC. Genotypic and Phenotypic Characterization of Penicillium marneffei Mutants Produced by Agrobacterium-Mediated Transformation. [Masters Thesis]. Youngstown State University; 2012. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1341255805

University of Melbourne

8. Lelwala, Lelwala Liyana Arachchige Ruvini Vinodya. Genomics and population biology of the anthracnose pathogen (Colletotrichum tanaceti) of pyrethrum in Australia.

Degree: 2019, University of Melbourne

URL: http://hdl.handle.net/11343/230875

► Colletotrichum tanaceti, is an emerging fungal pathogen of pyrethrum (Tanacetum cinerariifolium) in Australia. This thesis investigated the evolutionary potential and the molecular bases of sexual… (more)

▼ Colletotrichum tanaceti, is an emerging fungal pathogen of pyrethrum (Tanacetum cinerariifolium) in Australia. This thesis investigated the evolutionary potential and the molecular bases of sexual reproduction and pathogenicity of C. tanaceti by combining genomics, comparative genomics, molecular genetics and population genetics, with molecular biology and mycology techniques. The whole genome of the holotype isolate of C. tanaceti, BRIP57314 was sequenced, assembled and annotated then supplemented with a RNA transcriptome thus providing the first public genome sequence of C. tanaceti. The repetitive elements in the genome were identified in silico. Comparative genomics identified a relatively high repeat content therefore, suggesting a high genomic plasticity of the C. tanaceti genome. The bipartite nature of the C. tanaceti genome with distinct gene-sparse repeat and A-T rich regions were identified using in silico methods. Signals of genome-wide repeat induced point mutation (RIP) were identified using RIP-indices. The phenomenon of RIP in C. tanaceti genome was therefore, hypothesized to cause the A-T rich regions that evolve at a higher rate than the rest of the genome, generating a ‘two-speed genome’. Genomics tools were used to compare the C. tanaceti genome to publicly available genomes of other Colletotrichum spp. Single-copy orthologs among the species were used to develop a phylogram. This was then converted to a chronogram to estimate the divergence of C. tanaceti from C. higginsianum at 9.97 million years ago. The genome sequence and the predicted proteome of C. tanaceti were utilized to identify the putative pathogenicity genes of C. tanaceti. A large array of putative genes involved in infection and colonization of the host tissue such as CAZymes, effectors, secondary metabolites, transporters, cytochrome P450 and proteases were identified in the C. tanaceti genome. This was achieved by comparing genome homology to database entries and bioinformatics tools for de novo identification of fungal pathogenicity genes. The comparison of the putative pathogenicity gene repertoire of C. tanaceti with other Colletotrichum spp. indicated C. tanaceti had a similar pathogenicity profile to Colletotrichum spp. with intermediate host ranges. Therefore, C. tanaceti was speculated to have potential hosts other than pyrethrum. Furthermore, the in silico analyses revealed that the pathogenicity genes of C. tanaceti are located significantly closer to the tandem repeats, transposable elements and the RIP-affected regions of the genome. This suggested the potential of these pathogenicity genes to diversify and evolve adaptively. The population biology of C. tanaceti was assessed using a neutral, polymorphic microsatellite marker library developed from the draft genome sequence of C. tanaceti. Symptomatic pyrethrum leaves were hierarchically sampled across pyrethrum growing regions of Australia over three years in order to establish a collection of C. tanaceti isolates. These isolates were genotyped using the…

Subjects/Keywords: Colletotrichum tanaceti; Pyrethrum; Evolutionary potential; Fungal genomics; Fungal population biology; Fungal sexual reproduction

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APA (6^th Edition):

Lelwala, L. L. A. R. V. (2019). Genomics and population biology of the anthracnose pathogen (Colletotrichum tanaceti) of pyrethrum in Australia. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/230875

Chicago Manual of Style (16^th Edition):

Lelwala, Lelwala Liyana Arachchige Ruvini Vinodya. “Genomics and population biology of the anthracnose pathogen (Colletotrichum tanaceti) of pyrethrum in Australia.” 2019. Doctoral Dissertation, University of Melbourne. Accessed January 23, 2021. http://hdl.handle.net/11343/230875.

MLA Handbook (7^th Edition):

Lelwala, Lelwala Liyana Arachchige Ruvini Vinodya. “Genomics and population biology of the anthracnose pathogen (Colletotrichum tanaceti) of pyrethrum in Australia.” 2019. Web. 23 Jan 2021.

Vancouver:

Lelwala LLARV. Genomics and population biology of the anthracnose pathogen (Colletotrichum tanaceti) of pyrethrum in Australia. [Internet] [Doctoral dissertation]. University of Melbourne; 2019. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11343/230875.

Council of Science Editors:

Lelwala LLARV. Genomics and population biology of the anthracnose pathogen (Colletotrichum tanaceti) of pyrethrum in Australia. [Doctoral Dissertation]. University of Melbourne; 2019. Available from: http://hdl.handle.net/11343/230875

9. Esher, Shannon. Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition .

Degree: 2018, Duke University

URL: http://hdl.handle.net/10161/16829

► Cryptococcus neoformans is an opportunistic fungal pathogen that causes significant disease and death in immunocompromised populations, in particular among those with advanced HIV infection.… (more)

▼ Cryptococcus neoformans is an opportunistic fungal pathogen that causes significant disease and death in immunocompromised populations, in particular among those with advanced HIV infection. This fungus is found ubiquitously in the environment and acquired through inhalation into the respiratory tract followed by dissemination to the central nervous system in immunocompromised individuals. The ability for C. neoformans to sense and adapt to the host environment is crucial to its success as a pathogen. Many C. neoformans proteins require proper subcellular localization for their function, and as such this fungus carefully regulates the localization of proteins involved in important cellular processes related to host adaptation. Fungal growth and morphogenesis, as well as thermotolerance and virulence are controlled by conserved Ras-like GTPases. These proteins require proper localization for full function and are directed to cellular membranes through the posttranslational modification process known as prenylation. Using the tools of fungal genetics and molecular biology, we establish that the C. neoformans RAM1 gene encoding the farnesyltransferase -subunit is required for thermotolerance and pathogenesis. We also identified and characterized post-prenylation protease and carboxyl methyltransferase enzymes in C. neoformans, demonstrating that these later steps have only subtle effects on stress response and fungal virulence. By fluorescent microscopy and molecular biology, we show that Ram1 is required for proper subcellular localization of Ras1, but not Cdc42, and that the post-prenylation processing steps are dispensable for the localization of these substrate proteins. C. neoformans dramatically alters its cell wall upon entering the host in order to facilitate immune avoidance. Using the tools of forward genetics, we identified a novel cell wall regulatory protein, Mar1. We have demonstrated that this protein is required for capsule attachment and full virulence in mouse models of infection. Using staining and biochemical techniques, we have characterized the cell wall of mar1∆ mutant cells, and by fluorescent microscopy we have demonstrated that the -(1,3)-glucan synthase catalytic subunit, Fks1, is mislocalized in mar1∆ cells. Using in vitro co-culture models, we have determined that the mar1∆ cell wall induces increased macrophage activation that is dependent on the Card9 and MyD88 adaptor proteins, as well as the Dectin-1 and TLR-2 pattern recognition receptors. To further understand the impact of the Mar1 protein on the host-pathogen interaction, we used in vivo mouse models to characterize the pathogenesis and immune response to this strain. Using histopathology and light microscopy, we have shown that mar1∆ cells induce granulomas in the lungs of infected mice, an in in vitro co-culture models we have demonstrated that the mar1∆ strain induces increased markers angiogenesis. Finally using immunization strategies, we show that the mar1∆ strain does not induce a protective response… Advisors/Committee Members: Alspaugh, J Andrew (advisor).

Subjects/Keywords: Microbiology; Genetics; Molecular biology; Cell wall; Cryptococcus neoformans; Fungal genetics; Fungal immunology; Fungal pathogenesis; Prenylation

10 more images…

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APA (6^th Edition):

Esher, S. (2018). Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition . (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/16829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Esher, Shannon. “Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition .” 2018. Thesis, Duke University. Accessed January 23, 2021. http://hdl.handle.net/10161/16829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Esher, Shannon. “Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition .” 2018. Web. 23 Jan 2021.

Vancouver:

Esher S. Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition . [Internet] [Thesis]. Duke University; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10161/16829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Esher S. Mechanisms of Protein Localization in Cryptococcus neoformans Mediate Virulence and Immune Recognition . [Thesis]. Duke University; 2018. Available from: http://hdl.handle.net/10161/16829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

10. Suleiman, M.M. (Mohammed Musa). The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts .

Degree: 2010, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-10062010-203419/

► The main aim of this study was to find a plant extract or isolated compound that could be used to combat aspergillosis in animals. Aspergillus… (more)

▼ The main aim of this study was to find a plant extract or isolated compound that could be used to combat aspergillosis in animals. Aspergillus fumigatus is one of the most common pathogenic fungal species in humans and animals. A. fumigatus is also an economically important fungus in the poultry industry. Current treatment of the disease is hampered by drug resistance of the organism to conventional antifungals and also its widespread toxicity to the animals. Seven tree species that had good antifungal activity against Cryptococcus neoformans in the Phytomedicine Programme database were selected for further work. These tree species were: Combretum vendae A.E. van Wyk (Combretaceae), Commiphora harveyi (Engl.) Engl. (Burseraceae), Khaya anthotheca (Welm.) C.DC (Meliaceae), Kirkia wilmsii Engl. (Kirkiaceae), Loxostylis alata A. Spreng. ex Rchb. (Anacardiaceae), Ochna natalitia (Meisn.) Walp. (Ochnaceae) and Protorhus longifolia (Bernh. Ex C. Krauss) Engl. (Anacardiaceae). The antimicrobial activity of leaf extracts of the selected plant species were determined against four important nosocomial bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa) and five important animal fungi (Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Microsporum canis and Sporothrix schenckii) using a serial microplate dilution method. The minimal inhibitory concentrations (MIC), of an acetone extract of Loxostylis alata was the lowest against Aspergillus fumigatus with an MIC value of 0.05 mg/ml. The number of antifungal compounds in extracts was determined by bioautography. The acetone extract of L. alata had the most active zones (10). The antioxidant, antiplatelet and cytotoxic effects of the seven plant species were evaluated using established in vitro assays. All the extracts had comparably low toxicity except for the extract of C. harveyi that had high haemagluttination assay titre value, which indicates toxicity. The extracts of P. longifolia, K. wilmsii, O. natalitia, L. alata, C. harveyi and C. vendae contained antioxidant compounds in the qualitative assay using DPPH. In the quantification of antioxidation using ABTS, only the extracts of P. longifolia, L. alata, and C. vendae had substantial antioxidant activity with respective TEAC value of 1.39, 1.94 and 2.08. Similarly, in the quantitative DPPH assay, L. alata. (EC50, 3.58 ± 0.23 μg/ml) and K. wilmsii (EC50, 3.57 ± 0.41 μg/ml) did not differ significantly (p ≤ 0.05) from the positive control (L-ascorbic acid). K. anthotheca had a much lower antioxidant activity (EC<su>50 176.40 ± 26.56 μg/ml), and differed significantly (p ≤ 0.05) from all the other extracts and control. In addition, the extract of C. vendae and C. harveyi had significant (p ≤ 0.05) antiplatelet activity and did not differ from the control (aspirin) with EC50 of 0.06 ± 0.01 μg/ml, 0.19 ± 0.00 μg/ml, respectively. Lower EC50 values in the antioxidant and antiplatelet studies are indicative of superior activity of the plant extract against oxidation… Advisors/Committee Members: Naidoo, Vinny (advisor), Eloff, Jacobus Nicolaas (advisor).

Subjects/Keywords: Aspergillus fumigatus; Fungal species; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suleiman, M. M. (. M. (2010). The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-10062010-203419/

Chicago Manual of Style (16^th Edition):

Suleiman, M M (Mohammed Musa). “The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts .” 2010. Doctoral Dissertation, University of Pretoria. Accessed January 23, 2021. http://upetd.up.ac.za/thesis/available/etd-10062010-203419/.

MLA Handbook (7^th Edition):

Suleiman, M M (Mohammed Musa). “The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts .” 2010. Web. 23 Jan 2021.

Vancouver:

Suleiman MM(M. The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts . [Internet] [Doctoral dissertation]. University of Pretoria; 2010. [cited 2021 Jan 23]. Available from: http://upetd.up.ac.za/thesis/available/etd-10062010-203419/.

Council of Science Editors:

Suleiman MM(M. The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts . [Doctoral Dissertation]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-10062010-203419/

Oregon State University

11. Hill, David E. (David Edwin) 1954-. Approaches to the total synthesis of verrucarol.

Degree: PhD, Chemistry, 1986, Oregon State University

URL: http://hdl.handle.net/1957/40110

► Verrucarol is the sesquiterpene portion of a number of macrocyclic di- and triesters of potential medicinal importance. In this study, a novel approach to the… (more)

Subjects/Keywords: Fungal metabolites

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APA (6^th Edition):

Hill, D. E. (. E. 1. (1986). Approaches to the total synthesis of verrucarol. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/40110

Chicago Manual of Style (16^th Edition):

Hill, David E (David Edwin) 1954-. “Approaches to the total synthesis of verrucarol.” 1986. Doctoral Dissertation, Oregon State University. Accessed January 23, 2021. http://hdl.handle.net/1957/40110.

MLA Handbook (7^th Edition):

Hill, David E (David Edwin) 1954-. “Approaches to the total synthesis of verrucarol.” 1986. Web. 23 Jan 2021.

Vancouver:

Hill DE(E1. Approaches to the total synthesis of verrucarol. [Internet] [Doctoral dissertation]. Oregon State University; 1986. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1957/40110.

Council of Science Editors:

Hill DE(E1. Approaches to the total synthesis of verrucarol. [Doctoral Dissertation]. Oregon State University; 1986. Available from: http://hdl.handle.net/1957/40110

University of Saskatchewan

12. Azad, Kumkum 1985-. Fungal endophytes that confer tolerance to salt and dry conditions.

Degree: 2016, University of Saskatchewan

URL: http://hdl.handle.net/10388/12592

► Fungal endophytes grow symbiotically inside plants, where some strains promote plant growth and survival under particular abiotic stresses. Tomato (Solanum lycopersicum Var. Rutgers) seeds were… (more)

▼ Fungal endophytes grow symbiotically inside plants, where some strains promote plant growth and survival under particular abiotic stresses. Tomato (Solanum lycopersicum Var. Rutgers) seeds were inoculated with systemic (also called class 2) fungal endophytes (Alternaria spp and Trichoderma harzianum). These endophytes were isolated from plants naturally growing in salinized-soil in Saskatchewan, e.g. Little Manitou Lake shore, Radisson Lake shore, and Mosaic Belle Plaine tailings area. The effects of colonization with systemic fungal endophytes were studied on growth performance of tomato plants under NaCl and drought stress. Endophyte-colonized plants had greater fresh shoot biomass than control plants after 20 d of NaCl stress (300 and 500 mM). They also maintained greater fresh root biomass after 10 d NaCl stress (300 mM). After exposure to chronic 100 mM and 200 mM NaCl stress, there was no remarkable difference in plant biomass (both root and shoot) between endophyte-colonized plants and non-colonized control plants. Exposure to NaCl stress altered different aspects of the plants’ physiology such as photosynthetic efficiency, osmolyte adjustment, and reactive oxygen species generation. Photosynthetic efficiency was improved by endophyte colonization during chronic NaCl stress, but decreased significantly during ≥400 mM NaCl stress. Although osmolality of plants increased with the increase of NaCl salinity, there was no effect of endophyte colonization on plant osmolality. On the other hand, reactive oxygen species activity of endophyte-colonized plants was always lower in comparison to non-colonized control plants in response to NaCl stress. Endophyte-colonized plants growth performance and physiological responses were also determined under drought. Endophyte-colonized plants had significantly higher shoot biomass in comparison to non-colonized control plants after intermittent drought and continuous drought. Physiological responses of plants differed following intermittent and continuous drought stress. Photosynthetic efficiency of endophyte-colonized plants improved significantly after intermittent drought, but there was no effect of endophyte colonization on photosynthetic efficiency of plants after continuous drought. On the other hand, increased proline accumulation and decreased osmolyte concentration were observed in endophyte-colonized plants in response to drought stress. There was also indication of less reactive oxygen species in endophyte-colonized plants upon drought stress. Finally, better fluid use efficiency of endophyte-colonized plants was observed, which is correlated to drought tolerance in endophyte-colonized plants. These results suggest that our systemic fungal endophytes have the potential to improve agriculture and horticulture on salinized and dry soils which are common phenomenon in semi-arid environments. Advisors/Committee Members: Kaminskyj, Susan, Basinger, Jim, Wilson, Ken, Knight, Diane.

Subjects/Keywords: Fungal endophytes; salt stress; drought

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APA (6^th Edition):

Azad, K. 1. (2016). Fungal endophytes that confer tolerance to salt and dry conditions. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12592

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Azad, Kumkum 1985-. “Fungal endophytes that confer tolerance to salt and dry conditions.” 2016. Thesis, University of Saskatchewan. Accessed January 23, 2021. http://hdl.handle.net/10388/12592.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Azad, Kumkum 1985-. “Fungal endophytes that confer tolerance to salt and dry conditions.” 2016. Web. 23 Jan 2021.

Vancouver:

Azad K1. Fungal endophytes that confer tolerance to salt and dry conditions. [Internet] [Thesis]. University of Saskatchewan; 2016. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10388/12592.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Azad K1. Fungal endophytes that confer tolerance to salt and dry conditions. [Thesis]. University of Saskatchewan; 2016. Available from: http://hdl.handle.net/10388/12592

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Bradley, Devon J. Causes and consequences of microbial diversity: from marshes to model systems.

Degree: PhD, Division of Biology and Medicine. Ecological and Evolutionary Biology, 2008, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:19/

► I investigated factors that influence microbial diversity and composition and the consequences of this diversity for plant communities. First, I used cloning and sequencing of… (more)

Subjects/Keywords: fungal diversity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bradley, D. J. (2008). Causes and consequences of microbial diversity: from marshes to model systems. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:19/

Chicago Manual of Style (16^th Edition):

Bradley, Devon J. “Causes and consequences of microbial diversity: from marshes to model systems.” 2008. Doctoral Dissertation, Brown University. Accessed January 23, 2021. https://repository.library.brown.edu/studio/item/bdr:19/.

MLA Handbook (7^th Edition):

Bradley, Devon J. “Causes and consequences of microbial diversity: from marshes to model systems.” 2008. Web. 23 Jan 2021.

Vancouver:

Bradley DJ. Causes and consequences of microbial diversity: from marshes to model systems. [Internet] [Doctoral dissertation]. Brown University; 2008. [cited 2021 Jan 23]. Available from: https://repository.library.brown.edu/studio/item/bdr:19/.

Council of Science Editors:

Bradley DJ. Causes and consequences of microbial diversity: from marshes to model systems. [Doctoral Dissertation]. Brown University; 2008. Available from: https://repository.library.brown.edu/studio/item/bdr:19/

Addis Ababa University

14. GEBREABIEZGI, TEKLEBIRHAN. PREVALENCE OF DERMATOPHYTES AND NON-DERMATOPHYTE FUNGAL INFECTION AMONG PATIENTS VISITING DERMATOLOGY CLINIC, AT TIKUR ANBESSA HOSPITAL, ADDIS ABABA, ETHIOPIA.

Degree: 2014, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/5375

► Abstract Back ground: Dermatomycosis is a common fungal infection that constitutes public health problem among humans and animals worldwide, including Ethiopia. Though it is a… (more)

▼ Abstract Back ground: Dermatomycosis is a common fungal infection that constitutes public health problem among humans and animals worldwide, including Ethiopia. Though it is a trivial disease, its psychological effect and morbidity in terms of loss of time and treatment cost is considerable. Objective: To describe the most dominant clinical manifestation, the dominant fungi implicated as a cause of dermatomycosis and determine the prevalence of dermatophytes, non- dermatophyte fungi and yeasts collected from clinical samples suspected of dermatomycosis. Material and methods: a cross sectional descriptive study design from January to May, 2014 was conducted at Tikur Anbessa Hospital. Scrapings from skin nail and scalp of 305 study participants was collected by employing standard routine microbiological techniques. A portion of each sample was placed on a slide and a drop of an aqueous solution of 10% (w/v) potassium hydroxide, was added. After 5 minutes, the wet mount was examined under low (X10) and high (X40) power magnification for the presence of fungal elements. The remaining portion of each clinical sample was cultured irrespective of the negative or positive direct microscopic examination results. Each sample was streaked on two plates of Sabouraud’s dextrose agar (SDA) with chloramphenicol and SDA, with chloramphenicol and cychloheximide which were prepared according to the manufacture’s instruction. All inoculated plates were then incubated at inverted position for 4-6 weeks at 25-30 0 C aerobically. Incubated plates were examined twice a week for any fungal growth. Colonies suspected of dermatophytes were sub-cultured into potato dextrose agar for the production of spores. Mold isolates were identified by examining macroscopic and microscopic characteristics of their colony. Microscopic identification of mold isolates was performed by placing pieces cultures from SDA and/or PDA to clean microscopic slide and staining with lactophenol cotton blue. After placing a cover slip, each preparation was observed microscopically. Yeast identification, C. albicans was differentiated from other yeasts by germ tube production. Data was analyzed using SPSS version 20 software. Result: A total of 305, study participants were enrolled in the present study of which 97 (31.8%) were males and 208 (68.2%) females. The ages of study subjects ranged from 1 to 80 year with a mean age of 26 years. Out of the 305 study subjects, fungal species were detected (direct microscopy) in 166 (54.4%) of clinical samples while 242(79.3%) clinical samples were culture positive. Sixteen clinical samples that were culture negative were positive by direct microscopy. The three predominant clinical manifestation were tinea ungium accounting 156(51.1%) of iii clinical manifestations of which 119 (76.3%) were in females and 37(23.7%) in males. This was followed by tinea capitis accounting 61 (20%) of which 37 (60.7%) in females and 24 (39.3%) in males followed tinea corporis accounting 33(10.8%) of… Advisors/Committee Members: ADANE BITEW (MSc, PhD) (advisor).

Subjects/Keywords: DERMATOPHYTES; NON-DERMATOPHYTE FUNGAL INFECTION

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APA (6^th Edition):

GEBREABIEZGI, T. (2014). PREVALENCE OF DERMATOPHYTES AND NON-DERMATOPHYTE FUNGAL INFECTION AMONG PATIENTS VISITING DERMATOLOGY CLINIC, AT TIKUR ANBESSA HOSPITAL, ADDIS ABABA, ETHIOPIA. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5375

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

GEBREABIEZGI, TEKLEBIRHAN. “PREVALENCE OF DERMATOPHYTES AND NON-DERMATOPHYTE FUNGAL INFECTION AMONG PATIENTS VISITING DERMATOLOGY CLINIC, AT TIKUR ANBESSA HOSPITAL, ADDIS ABABA, ETHIOPIA. ” 2014. Thesis, Addis Ababa University. Accessed January 23, 2021. http://etd.aau.edu.et/dspace/handle/123456789/5375.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

GEBREABIEZGI, TEKLEBIRHAN. “PREVALENCE OF DERMATOPHYTES AND NON-DERMATOPHYTE FUNGAL INFECTION AMONG PATIENTS VISITING DERMATOLOGY CLINIC, AT TIKUR ANBESSA HOSPITAL, ADDIS ABABA, ETHIOPIA. ” 2014. Web. 23 Jan 2021.

Vancouver:

GEBREABIEZGI T. PREVALENCE OF DERMATOPHYTES AND NON-DERMATOPHYTE FUNGAL INFECTION AMONG PATIENTS VISITING DERMATOLOGY CLINIC, AT TIKUR ANBESSA HOSPITAL, ADDIS ABABA, ETHIOPIA. [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2021 Jan 23]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5375.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

GEBREABIEZGI T. PREVALENCE OF DERMATOPHYTES AND NON-DERMATOPHYTE FUNGAL INFECTION AMONG PATIENTS VISITING DERMATOLOGY CLINIC, AT TIKUR ANBESSA HOSPITAL, ADDIS ABABA, ETHIOPIA. [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5375

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

15. Birhan, Moges. Species Distribution and In vitro Antifungal Susceptibility of Oropharyngeal Yeast Isolates from HIV Patients in Zewditu Memorial Hospital, Addis Ababa, Ethiopia .

Degree: 2014, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/5681

► Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection of the oral cavity in HIV infected patients. OPC is associated with increased morbidity… (more)

▼ Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection of the oral cavity in HIV infected patients. OPC is associated with increased morbidity and mortality in immunosuppressed patients. However, data on Species distribution and antifungal susceptibility profiles of yeast isolated from HIV-infected patients in Ethiopia is limited. Objective: The aim of the study was to determine the spectrum of Oropharyngeal yeast isolates from HIV patients and to evaluate antifungal drug susceptibility profile of yeast isolates to commonly used antifungal drugs. Method: A cross sectional study was conducted in Zewditu Memorial Hospital, Addis Ababa. Oral swabs were collected from 224 HIV patients and cultured on Sabouraud Dextrose Agar with chloramphenicol. All the isolates were characterized to a species level following standard microbiological techniques. The antifungal susceptibility profiles of all yeast were determined using disk diffusion method. Result: Of 224 HIV patients screened, 139 (62.1%) were colonized by yeasts. The rate of colonization is higher in patients that non HAART than HAART initiated (72.3% versus 51.8%). A total of 155 yeasts were isolated of which 153 (98.7%) were accounted by Candida consisting of five species and 2(1.3%) non-candida yeasts. Out of all isolates C. albicans was the most frequently isolated species accounting 68.4% followed by C. glabrata (15.5%) and C. tropicalis (11%). The Antifungal susceptibility pattern of the yeast isolates for Fluconazole, ketoconazole, Amphotericin B, Clotrimazole, Nystatin and Miconazole showed a resistance of 12.3%, 8.4%, 5.8%, 2.6%, 1.3%, and 0.6% respectively. C. albicans were 5.7% resistant to ketoconazole and 6.6% resistant to Fluconazole. C. glabrata and C. tropicalis were 12.5% and 17.6% resistant to ketoconazole and 16.6% and 35.3% resistant to fluconazole respectively. vii Conclusion: The spectrum of yeast isolated in the present study was relatively high even if C. albicans was the predominantly isolated yeast. The results of this study also revealed that development of drug resistance by yeast isolates were considerable. Isolation, characterization and evaluating drug susceptibility pattern of the isolates in this hospital in particular and in health institutions through out of the country in general is recommended. Advisors/Committee Members: Adane Bitew (MSc, PhD) (advisor).

Subjects/Keywords: Oropharyngeal Candidiasis (OPC); fungal infection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Birhan, M. (2014). Species Distribution and In vitro Antifungal Susceptibility of Oropharyngeal Yeast Isolates from HIV Patients in Zewditu Memorial Hospital, Addis Ababa, Ethiopia . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Birhan, Moges. “Species Distribution and In vitro Antifungal Susceptibility of Oropharyngeal Yeast Isolates from HIV Patients in Zewditu Memorial Hospital, Addis Ababa, Ethiopia .” 2014. Thesis, Addis Ababa University. Accessed January 23, 2021. http://etd.aau.edu.et/dspace/handle/123456789/5681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Birhan, Moges. “Species Distribution and In vitro Antifungal Susceptibility of Oropharyngeal Yeast Isolates from HIV Patients in Zewditu Memorial Hospital, Addis Ababa, Ethiopia .” 2014. Web. 23 Jan 2021.

Vancouver:

Birhan M. Species Distribution and In vitro Antifungal Susceptibility of Oropharyngeal Yeast Isolates from HIV Patients in Zewditu Memorial Hospital, Addis Ababa, Ethiopia . [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2021 Jan 23]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Birhan M. Species Distribution and In vitro Antifungal Susceptibility of Oropharyngeal Yeast Isolates from HIV Patients in Zewditu Memorial Hospital, Addis Ababa, Ethiopia . [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

16. Fan, Ying. SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA.

Degree: PhD, Department of Physics, 2011, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/2924

► Microbial rhodopsins are widespread bacteriorhodopsin-like proteins found in many prokaryotes and lower eukaryotic groups. They serve as photosensors, light-driven ion pumps, and light-gated channels. The… (more)

▼ Microbial rhodopsins are widespread bacteriorhodopsin-like proteins found in many prokaryotes and lower eukaryotic groups. They serve as photosensors, light-driven ion pumps, and light-gated channels. The main goal of this thesis was to spectroscopically characterize a new subgroup of fungal rhodopsins (so-called auxiliary group) and a new type of rhodopsin found in flavobacteria. Towards the first goal, products of two known rhodopsin genes from the fungal wheat pathogen, Phaeosphaeria nodorum, were investigated. The two yeast-expressed Phaeosphaeria rhodopsins were spectroscopically characterized by Raman, time-resolved visible and Fourier transform infrared (FTIR) spectroscopy and shown many similarities: absorption spectra, conformation of the retinal chromophore, fast photocycling, and carboxylic acid protonation changes. It is likely that both Phaeosphaeria rhodopsins are proton-pumping, at least in vitro. We suggest that auxiliary rhodopsins have separated from their ancestors fairly recently and have acquired the ability to interact with as yet unidentified transducers, performing a photosensory function without changing their spectral properties and basic photochemistry. In the second project, we studied a flavobacterial rhodopsin with highly unusual sequence, which was functionally expressed in E.coli by our collaborators and produced light-induced pH changes in the spheroplast suspensions, suggesting an inward H+ transport. Using time-resolved visible spectroscopy, we revealed that its photochemical reaction cycle was fast and strongly dependent on cations. Analysis of the sequence alignments, combined with visible and FTIR spectroscopic studies of the wild-type and mutant flavobacterial rhodopsin, provided the clues for the mechanism of binding metal ions and origin of the observed light-induced pH changes. Finally, isotope labeling protocol, previously used for soluble secreted proteins, was successfully implemented and optimized to produce homogeneous samples of eukaryotic rhodopsin from Leptosphaeria in methylotrophic yeast. Isotope-labeling extent and functionality were verified by FTIR spectroscopy, and obtained samples gave high-resolution ssNMR spectra suitable for structural studies. This protocol for overexpression of isotope-labeled multi-spanning eukaryotic membrane proteins in Pichia pastoris can be adopted for challenging mammalian targets, which often resist characterization by other structural methods. Advisors/Committee Members: Brown, Leonid (advisor).

Subjects/Keywords: microbial rhodopsin; fungal; flavobacteria; spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fan, Y. (2011). SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/2924

Chicago Manual of Style (16^th Edition):

Fan, Ying. “SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA.” 2011. Doctoral Dissertation, University of Guelph. Accessed January 23, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/2924.

MLA Handbook (7^th Edition):

Fan, Ying. “SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA.” 2011. Web. 23 Jan 2021.

Vancouver:

Fan Y. SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA. [Internet] [Doctoral dissertation]. University of Guelph; 2011. [cited 2021 Jan 23]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/2924.

Council of Science Editors:

Fan Y. SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA. [Doctoral Dissertation]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/2924

University of Guelph

17. de la Cerda, Karla Adriana. Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs.

Degree: MS, Department of Environmental Biology, 2011, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034

► The species complex, Waitea circinata (WC) has been currently divided into five cultural types: var. circinata, var. oryzae, var. zeae, var. agrostis, and var. prodigus.… (more)

Subjects/Keywords: Fungal; Rhizoctonia; Pathogenicity; Taxonomy; Phylogenetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

de la Cerda, K. A. (2011). Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034

Chicago Manual of Style (16^th Edition):

de la Cerda, Karla Adriana. “Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs.” 2011. Masters Thesis, University of Guelph. Accessed January 23, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034.

MLA Handbook (7^th Edition):

de la Cerda, Karla Adriana. “Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs.” 2011. Web. 23 Jan 2021.

Vancouver:

de la Cerda KA. Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs. [Internet] [Masters thesis]. University of Guelph; 2011. [cited 2021 Jan 23]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034.

Council of Science Editors:

de la Cerda KA. Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs. [Masters Thesis]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034

University of Texas Southwestern Medical Center

18. Semeiks, Jeremy Raymond. On Two Problems in Comparative Genomics of Eukaryotes.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/ETD-UTSWMED-2015-05-94

► The recent advent of whole-genome sequencing allows us to use novel comparative methods to explore the genetic bases for traits of interest. Here, I present… (more)

▼ The recent advent of whole-genome sequencing allows us to use novel comparative methods to explore the genetic bases for traits of interest. Here, I present two case studies of such methods applied to eukaryote genomes. The first study regards the evolution of longevity in the mammalian proteome. Evolutionary theory suggests that the force of natural selection decreases with age. To explore the extent to which this prediction directly affects protein structure and function, I used computational methods to identify positions of proteins conserved in long-lived but not in short-lived mammal species. I analyzed 7,590 orthologous protein families in 33 mammalian species, accounting for body mass, phylogeny, and species-specific mutation rate. Overall, I found that the number of longevity-selected positions in the mammalian proteome is much greater than would be expected by chance. Further, these positions are enriched in domains of several proteins that interact with one another in inflammation and other aging-related processes, as well as in organismal development. I present as an example the kinase domain of anti-Müllerian hormone type-2 receptor (AMHR2). AMHR2 inhibits ovarian follicle recruitment and growth, and my results show that its longevity-selected positions cluster near a SNP associated with delayed human menopause. Distinct from its canonical role in development, this region of AMHR2 may function to regulate the protein's activity in a lifespan-specific manner. The second study concerns the genetic basis for toxin production in the black mold genus Stachybotrys, which produces several diverse toxins that can damage human health. Its strains comprise two mutually-exclusive toxin chemotypes, one producing satratoxins (a subclass of trichothecenes) and the other producing the less-toxic atranones. To determine the genetic bases for chemotype-specific differences in toxin production, I sequenced and assembled de novo four Stachybotrys genomes, including two from atranone strains and two from satratoxin strains. Comparative analysis of these four 35-Mbp genomes revealed several chemotype-specific gene clusters that are predicted to make atranones and satratoxins, based on several lines of evidence. I show that chemotype-specific gene clusters are likely the genetic basis for the mutually-exclusive toxin chemotypes of Stachybotrys. I then present a unified biochemical model for Stachybotrys toxin production. Advisors/Committee Members: Albanesi, Joseph P., Grishin, Nick V., Cowell, Lindsay G., Otwinowski, Zbyszek, Wright, Woodring E..

Subjects/Keywords: Genes, Fungal; Multigene Family; Stachybotrys

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Semeiks, J. R. (2013). On Two Problems in Comparative Genomics of Eukaryotes. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/ETD-UTSWMED-2015-05-94

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Semeiks, Jeremy Raymond. “On Two Problems in Comparative Genomics of Eukaryotes.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed January 23, 2021. http://hdl.handle.net/2152.5/ETD-UTSWMED-2015-05-94.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Semeiks, Jeremy Raymond. “On Two Problems in Comparative Genomics of Eukaryotes.” 2013. Web. 23 Jan 2021.

Vancouver:

Semeiks JR. On Two Problems in Comparative Genomics of Eukaryotes. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2015-05-94.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Semeiks JR. On Two Problems in Comparative Genomics of Eukaryotes. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2015-05-94

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

19. Chen, Rui, 1963-. Isolation of protoplasts from selected wood degrading fungi and their uses in studying fungicidal action.

Degree: MS, Forest Products, 1992, Oregon State University

URL: http://hdl.handle.net/1957/18322

► Studies of physiological effects of fungicides on fungi are critical for understanding modes of action and mechanism of resistance; however, studies are difficult to perform… (more)

Subjects/Keywords: Fungal protoplasts

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APA (6^th Edition):

Chen, Rui, 1. (1992). Isolation of protoplasts from selected wood degrading fungi and their uses in studying fungicidal action. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/18322

Chicago Manual of Style (16^th Edition):

Chen, Rui, 1963-. “Isolation of protoplasts from selected wood degrading fungi and their uses in studying fungicidal action.” 1992. Masters Thesis, Oregon State University. Accessed January 23, 2021. http://hdl.handle.net/1957/18322.

MLA Handbook (7^th Edition):

Chen, Rui, 1963-. “Isolation of protoplasts from selected wood degrading fungi and their uses in studying fungicidal action.” 1992. Web. 23 Jan 2021.

Vancouver:

Chen, Rui 1. Isolation of protoplasts from selected wood degrading fungi and their uses in studying fungicidal action. [Internet] [Masters thesis]. Oregon State University; 1992. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1957/18322.

Council of Science Editors:

Chen, Rui 1. Isolation of protoplasts from selected wood degrading fungi and their uses in studying fungicidal action. [Masters Thesis]. Oregon State University; 1992. Available from: http://hdl.handle.net/1957/18322

Cranfield University

20. Parra, Roberto. Optimization of bioprocess design for pharmaceutical metabolites and enzymes.

Degree: PhD, Cranfield University at Silsoe; Institute of BioScience and Technology, 2004, Cranfield University

URL: http://hdl.handle.net/1826/782

► This study examines the effect of ecophysiology on growth of cells and production of enzymes and secondary metabolites produced by the fungi Aspergillus niger (lysozyme)… (more)

▼ This study examines the effect of ecophysiology on growth of cells and production of enzymes and secondary metabolites produced by the fungi Aspergillus niger (lysozyme) and a Phoma sp. (squalestatin S1). The effect of interactions of water activity (aw) (0.99-0.90), temperature (20, 30 and 35°C) and modifying aw solute (glycerol, NaCl) on growth and sporulation of a wild type strain of Aspergillus niger (W) and two genetically engineered lysozyme producing strains (L11, B1) was examined for the first time. Maximum growth rates were achieved for both strains (L11 and B1) under moderate aw levels. Optimum conditions for growth of strain L11 were estimated by means of contour plot surfaces and found to be 0.965 aw with glycerol as a solute at 35ºC (10.5 mm day-1). A model combining the effect of aw and temperature on growth of strains of Aspergillus niger, and comparison with data on food spoilage moulds in the literature was developed. The growth of two strains of A. niger, as a function of temperature (25-30 °C) and aw (0.90-0.99) was developed. The estimation of the minimum aw (awmin) and optimal aw (awopt) levels were in accordance with data in the literature for a range of other Aspergillus and related species, regardless of the solutes used for aw modification. A central composition design was used to describe the effects of water activity (aw, 0.98, 0.97 and 0.96), inoculum size (2.7x105, 2.7x104 and 2.7x103 spores ml-1), and three autoclaving procedure (A = all components autoclaved together, B = medium autoclaved + maltose filtered and, C = medium autoclaved + maltose & soya milk filtered) on the production of lysozyme by two genetically-engineered strains of Aspergillus niger (B1 and L11) in a liquid culture fermentation. Although both strains produced similar lysozyme concentrations (15 mg l-1), different production patterns were found under the experimental conditions. However, strain B1 produced relatively higher amounts of lysozyme under water stress (0.96 aw) with all the substrates autoclaved together. Subsequently, a central composition design was used to investigate: different immobilized polymer types (alginate and pectate), polymer concentration (2 and 4% (w/v)), inoculum support ratios (1:2 and 1:4) and gel-inducing agent concentration (CaCl2, 2 and 3.5% (w/v)) on lysozyme production. Overall immobilization in Ca-pectate resulted in higher lysozyme production compared to immobilization in Ca-alginate. Similar effects were observed when the polymer concentration was reduced. A 13 fold higher lysozyme production was achieved with Ca-pectate in comparison to Ca-alginate (20-23 and 0.5-1.7 mg l-1 respectively). Polymer modifications also significantly affected the final pH and aw of the immobilized cell fermentation. The aw factor is a very significant parameter in the immobilization design. A combined statistical methodology of orthogonal design L27(313) and surface response methodology was applied to optimize the composition and concentration of a liquid fermentation medium for the production of…

Subjects/Keywords: Fungal growth

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parra, R. (2004). Optimization of bioprocess design for pharmaceutical metabolites and enzymes. (Doctoral Dissertation). Cranfield University. Retrieved from http://hdl.handle.net/1826/782

Chicago Manual of Style (16^th Edition):

Parra, Roberto. “Optimization of bioprocess design for pharmaceutical metabolites and enzymes.” 2004. Doctoral Dissertation, Cranfield University. Accessed January 23, 2021. http://hdl.handle.net/1826/782.

MLA Handbook (7^th Edition):

Parra, Roberto. “Optimization of bioprocess design for pharmaceutical metabolites and enzymes.” 2004. Web. 23 Jan 2021.

Vancouver:

Parra R. Optimization of bioprocess design for pharmaceutical metabolites and enzymes. [Internet] [Doctoral dissertation]. Cranfield University; 2004. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1826/782.

Council of Science Editors:

Parra R. Optimization of bioprocess design for pharmaceutical metabolites and enzymes. [Doctoral Dissertation]. Cranfield University; 2004. Available from: http://hdl.handle.net/1826/782

University of Manitoba

21. Karuppiah, Kannan. Detection of fungal infection in pulses using near infrared (NIR) hyperspectral imaging.

Degree: Biosystems Engineering, 2015, University of Manitoba

URL: http://hdl.handle.net/1993/30773

► Pulses are a major source of human protein intake nowadays and will continue to be so because of their high protein content. Pulse crops are… (more)

▼ Pulses are a major source of human protein intake nowadays and will continue to be so because of their high protein content. Pulse crops are members of the family Leguminosae. The five major pulse crops grown in Canada are chick peas, green peas, lentils, pinto bean and kidney beans. Over the past 20 years, Canada has emerged as the world’s largest exporter of lentils and one of world’s top five exporters of beans. These contribute more than $2 billion income to the Canadian economy. The major causes of fungal infection in these pulses are Aspergillus flavus and Penicillium commune. Early stages of fungal infections in pulses are not detectable with human eyes. Near infrared (NIR) hyperspectral imaging system is an advanced technique widely used for detection of insect infestation and fungal infection in cereal grains and oil seeds. A typical NIR instrument captures images across the electromagnetic spectrum at evenly spaced wavelengths from 700 to 2500 nm (a system at the University of Manitoba captures images in the 960 nm to 1700 nm range). From the captured images, the spatial relationships for different spectra in the neighborhood can be found allowing more elaborate spectral-spatial methods for a more accurate classification of the images. The primary objective of this study was to assess the feasibility of the NIR hyperspectral system to identify fungal infections in pulses. Hyperspectral images of healthy and fungal infected chick peas, green peas, lentils, pinto bean and kidney beans were acquired and features (statistical and histogram) were used to develop classification models to identify fungal infection caused by Aspergillus flavus and Penicillium commune. Images of healthy and fungal infected kernels were acquired at 2 week intervals (0, 2, 4, 6, 8 and 10 weeks from artificial inoculation). Six-way (healthy vs the five different stages of infection) and two-way (healthy vs every stage of infection) models were developed and classifications were done using linear discriminant analysis (LDA) and quadratic discriminant analysis (QDA) classifiers. The LDA classifier identified with 90-94% accuracy while using the six-way model, and with 98-100% accuracy when using the two-way models for all five types of pulses and for both types of fungal infections. The QDA classifier also showed promising results as it identified 85-90% while using the six-way model and 96-100% when using the two-way models. Hence, hyperspectral imaging is a promising and non-destructive method for the rapid detection of fungal infections in pulses, which cannot be detected using human eyes. Advisors/Committee Members: Jayas, Digvir (Biosystems Engineering) (supervisor), White, Noel (Biosystems Engineering) .

Subjects/Keywords: Hyperspectral imaging; fungal infection; pulses

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Karuppiah, K. (2015). Detection of fungal infection in pulses using near infrared (NIR) hyperspectral imaging. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/30773

Chicago Manual of Style (16^th Edition):

Karuppiah, Kannan. “Detection of fungal infection in pulses using near infrared (NIR) hyperspectral imaging.” 2015. Masters Thesis, University of Manitoba. Accessed January 23, 2021. http://hdl.handle.net/1993/30773.

MLA Handbook (7^th Edition):

Karuppiah, Kannan. “Detection of fungal infection in pulses using near infrared (NIR) hyperspectral imaging.” 2015. Web. 23 Jan 2021.

Vancouver:

Karuppiah K. Detection of fungal infection in pulses using near infrared (NIR) hyperspectral imaging. [Internet] [Masters thesis]. University of Manitoba; 2015. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1993/30773.

Council of Science Editors:

Karuppiah K. Detection of fungal infection in pulses using near infrared (NIR) hyperspectral imaging. [Masters Thesis]. University of Manitoba; 2015. Available from: http://hdl.handle.net/1993/30773

22. Manzanares Miralles, Lara. Investigating and exploiting fungal natural products in Aspergillus spp.

Degree: 2015, RIAN

URL: http://eprints.maynoothuniversity.ie/7123/

► Gliotoxin (GT) is a redox-active natural product produced by some fungal species that contributes to the virulence of the human pathogen Aspergillus fumigatus. It contains… (more)

▼ Gliotoxin (GT) is a redox-active natural product produced by some fungal species that contributes to the virulence of the human pathogen Aspergillus fumigatus. It contains a characteristic disulphide bridge responsible for the deleterious effects of this molecule. GT also displays antifungal properties, however the mechanisms of cytotoxicity behind it has not been fully characterised. The work presented here investigates A. niger as a model organism to understand GT cytotoxicity and reveal new metabolic systems, as it does not produce GT. Comparative proteomics revealed that exposure of A. niger to exogenous GT resulted in a significant (p < 0.05) dysregulation of hundreds of proteins involved in several cellular processes, especially metabolism. Two putative S-adenosylmethionine (SAM)-dependent methyltransferases showed de novo abundance under GT conditions. In addition an increase in abundance of proteins involved in the methionine cycle was also observed. Analysis of methionine-related metabolites revealed significant increases in the levels of methionine and adenosine, in correlation with proteomic data. Moreover, proteomic data revealed a significant increase in the abundance of hydrolytic enzymes, including glycoside hydrolases (n = 22) and peptidases (n = 16) which have important applications in the biotechnology industry. A significant dysregulation of proteins involved in amino acid metabolism coupled to an increase in the levels of selected amino acids appeared to occur in A. niger under GT conditions. Furthermore this thesis reveals a novel protection mechanism in A. niger against GT which is mediated by one the methyltransferases (MTases) identified on the proteomic data. This MTase (referred as MT-II) is responsible for bisthiobis(methylthio)gliotoxin (BmGT) formation and deletion of MT-II led to increased GT sensitivity in A. niger. The applications of other fungal natural products like siderophores (Fusarinine C), are also evaluated herein. The development and validation of a Fusarinine C based enzyme-linked immunosorbent assay (ELISA) was carried out. Results showed the potential applications of this ELISA in the diagnosis of aspergillus related diseases. Overall, this work highlights the different roles and applications fungal natural products have.

Subjects/Keywords: fungal natural products; aspergillus spp.

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APA (6^th Edition):

Manzanares Miralles, L. (2015). Investigating and exploiting fungal natural products in Aspergillus spp. (Thesis). RIAN. Retrieved from http://eprints.maynoothuniversity.ie/7123/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Manzanares Miralles, Lara. “Investigating and exploiting fungal natural products in Aspergillus spp.” 2015. Thesis, RIAN. Accessed January 23, 2021. http://eprints.maynoothuniversity.ie/7123/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Manzanares Miralles, Lara. “Investigating and exploiting fungal natural products in Aspergillus spp.” 2015. Web. 23 Jan 2021.

Vancouver:

Manzanares Miralles L. Investigating and exploiting fungal natural products in Aspergillus spp. [Internet] [Thesis]. RIAN; 2015. [cited 2021 Jan 23]. Available from: http://eprints.maynoothuniversity.ie/7123/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Manzanares Miralles L. Investigating and exploiting fungal natural products in Aspergillus spp. [Thesis]. RIAN; 2015. Available from: http://eprints.maynoothuniversity.ie/7123/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

23. Mackenzie, Ashleigh. The role of Rhynchosporium commune cell wall components in cell wall integrity and pathogenicity.

Degree: PhD, 2014, University of Aberdeen

URL: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152632860005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.646091

► Rhynchosporium commune is one of the most destructive pathogens of barley worldwide. It can cause crop yield losses of up to 40% in the UK… (more)

▼ Rhynchosporium commune is one of the most destructive pathogens of barley worldwide. It can cause crop yield losses of up to 40% in the UK and decrease in grain quality. Populations of R. commune can change rapidly, defeating new barley resistance (R) genes and fungicides after just a few seasons of their use. Fungicide use is one of the major modes of management of Rhynchosporium and is heavily relied on the agricultural industry. Fungicides that were effective in the past are no longer effective in controlling the disease and many are only effective when used in mixtures. Beyond the currently effective fungicides there is limited new chemistry available so there is a very real need for development in this area. In pathogenic fungi, the cell wall components play a key role in the establishment of pathogenesis. The cell wall forms the outer structure protecting the fungus from the host defence mechanisms. It is involved in initiating the direct contact with the host cells by adhering to their surface. The fungal cell wall also contains important antigens and other compounds modulating host immune responses. R. commune germinated conidia and interaction transcriptome sequencing generated a list of over 30 different cell wall proteins (CWPs) potentially involved in pathogenicity. R. commune genome and interaction transcriptome sequencing provided further information about the extent of CWP families as well as a subset of genes expressed during barley colonisation by R. commune. The use of bioinformatic techniques allowed for the analysis of gene sequences. Putative cell wall associated genes were compared to the sequences from the fungal database via sequence similarity, sequence alignments 15 and conserved domain searches to better understand their function. Phylogenetic analysis also allowed us to understand the evolutionary relationship between R. commune genes and related genes in other organisms. Transcription profiling of R. commune CWPs during the development of infection helped to prioritise them for functional characterisation. Targeted gene disruption unfortunately did not yield mutants but has furthered our understanding of this technique in R. commune for future attempts. Functional complementation was successful however and allowed the uncovering of the function of RSA9. The results show that R. commune RSA9 functions as an allantoicase, an enzyme which breaks down purines as a source of nitrogen when conditions are nitrogen limited. The use of chemical cell wall inhibitors allowed us to better understand the role of carbohydrate cell wall components in R. commune fitness and virulence. Inhibition of cellulose production by DCB showed reduced growth, germination and pathogenicity of R. commune. Similar results were observed when beta-glucan synthesis was impaired; as inhibitor concentration increased, growth and germination of the fungus decreased. The composition of R. commune cell wall was also uncovered during this research. Techniques such as HPLC and FTIR eluded the composition of monosaccharides and…

Subjects/Keywords: 579; Rhynchosporium; Fungal cell walls

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mackenzie, A. (2014). The role of Rhynchosporium commune cell wall components in cell wall integrity and pathogenicity. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152632860005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.646091

Chicago Manual of Style (16^th Edition):

Mackenzie, Ashleigh. “The role of Rhynchosporium commune cell wall components in cell wall integrity and pathogenicity.” 2014. Doctoral Dissertation, University of Aberdeen. Accessed January 23, 2021. https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152632860005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.646091.

MLA Handbook (7^th Edition):

Mackenzie, Ashleigh. “The role of Rhynchosporium commune cell wall components in cell wall integrity and pathogenicity.” 2014. Web. 23 Jan 2021.

Vancouver:

Mackenzie A. The role of Rhynchosporium commune cell wall components in cell wall integrity and pathogenicity. [Internet] [Doctoral dissertation]. University of Aberdeen; 2014. [cited 2021 Jan 23]. Available from: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152632860005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.646091.

Council of Science Editors:

Mackenzie A. The role of Rhynchosporium commune cell wall components in cell wall integrity and pathogenicity. [Doctoral Dissertation]. University of Aberdeen; 2014. Available from: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152632860005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.646091

24. Manzanares Miralles, Lara. Investigating and exploiting fungal natural products in Aspergillus spp.

Degree: 2015, RIAN

URL: http://mural.maynoothuniversity.ie/7123/

► Gliotoxin (GT) is a redox-active natural product produced by some fungal species that contributes to the virulence of the human pathogen Aspergillus fumigatus. It contains… (more)

▼ Gliotoxin (GT) is a redox-active natural product produced by some fungal species that contributes to the virulence of the human pathogen Aspergillus fumigatus. It contains a characteristic disulphide bridge responsible for the deleterious effects of this molecule. GT also displays antifungal properties, however the mechanisms of cytotoxicity behind it has not been fully characterised. The work presented here investigates A. niger as a model organism to understand GT cytotoxicity and reveal new metabolic systems, as it does not produce GT. Comparative proteomics revealed that exposure of A. niger to exogenous GT resulted in a significant (p < 0.05) dysregulation of hundreds of proteins involved in several cellular processes, especially metabolism. Two putative S-adenosylmethionine (SAM)-dependent methyltransferases showed de novo abundance under GT conditions. In addition an increase in abundance of proteins involved in the methionine cycle was also observed. Analysis of methionine-related metabolites revealed significant increases in the levels of methionine and adenosine, in correlation with proteomic data. Moreover, proteomic data revealed a significant increase in the abundance of hydrolytic enzymes, including glycoside hydrolases (n = 22) and peptidases (n = 16) which have important applications in the biotechnology industry. A significant dysregulation of proteins involved in amino acid metabolism coupled to an increase in the levels of selected amino acids appeared to occur in A. niger under GT conditions. Furthermore this thesis reveals a novel protection mechanism in A. niger against GT which is mediated by one the methyltransferases (MTases) identified on the proteomic data. This MTase (referred as MT-II) is responsible for bisthiobis(methylthio)gliotoxin (BmGT) formation and deletion of MT-II led to increased GT sensitivity in A. niger. The applications of other fungal natural products like siderophores (Fusarinine C), are also evaluated herein. The development and validation of a Fusarinine C based enzyme-linked immunosorbent assay (ELISA) was carried out. Results showed the potential applications of this ELISA in the diagnosis of aspergillus related diseases. Overall, this work highlights the different roles and applications fungal natural products have.

Subjects/Keywords: fungal natural products; aspergillus spp.

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Manzanares Miralles, L. (2015). Investigating and exploiting fungal natural products in Aspergillus spp. (Thesis). RIAN. Retrieved from http://mural.maynoothuniversity.ie/7123/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Manzanares Miralles, Lara. “Investigating and exploiting fungal natural products in Aspergillus spp.” 2015. Thesis, RIAN. Accessed January 23, 2021. http://mural.maynoothuniversity.ie/7123/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Manzanares Miralles, Lara. “Investigating and exploiting fungal natural products in Aspergillus spp.” 2015. Web. 23 Jan 2021.

Vancouver:

Manzanares Miralles L. Investigating and exploiting fungal natural products in Aspergillus spp. [Internet] [Thesis]. RIAN; 2015. [cited 2021 Jan 23]. Available from: http://mural.maynoothuniversity.ie/7123/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Manzanares Miralles L. Investigating and exploiting fungal natural products in Aspergillus spp. [Thesis]. RIAN; 2015. Available from: http://mural.maynoothuniversity.ie/7123/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Palmer, Samuel Arrington. PESTICIDE TREATED CROP SEEDS AND TILLAGE ALTER SEED COAT FUNGAL COMMUNITIES ON AMARANTHUS RETROFLEXUS IN A MAIZE-SOYBEAN CROPPING SYSTEM.

Degree: MS, 2020, University of New Hampshire

URL: https://scholars.unh.edu/thesis/1392

► Soil fungi, by damaging or decaying weed seeds in the soil seed bank, are important agents of biological weed control. Pesticide seed treatments (PST)… (more)

Subjects/Keywords: Agroecology; Fungal Communities; Weed Science

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APA (6^th Edition):

Palmer, S. A. (2020). PESTICIDE TREATED CROP SEEDS AND TILLAGE ALTER SEED COAT FUNGAL COMMUNITIES ON AMARANTHUS RETROFLEXUS IN A MAIZE-SOYBEAN CROPPING SYSTEM. (Thesis). University of New Hampshire. Retrieved from https://scholars.unh.edu/thesis/1392

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Palmer, Samuel Arrington. “PESTICIDE TREATED CROP SEEDS AND TILLAGE ALTER SEED COAT FUNGAL COMMUNITIES ON AMARANTHUS RETROFLEXUS IN A MAIZE-SOYBEAN CROPPING SYSTEM.” 2020. Thesis, University of New Hampshire. Accessed January 23, 2021. https://scholars.unh.edu/thesis/1392.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Palmer, Samuel Arrington. “PESTICIDE TREATED CROP SEEDS AND TILLAGE ALTER SEED COAT FUNGAL COMMUNITIES ON AMARANTHUS RETROFLEXUS IN A MAIZE-SOYBEAN CROPPING SYSTEM.” 2020. Web. 23 Jan 2021.

Vancouver:

Palmer SA. PESTICIDE TREATED CROP SEEDS AND TILLAGE ALTER SEED COAT FUNGAL COMMUNITIES ON AMARANTHUS RETROFLEXUS IN A MAIZE-SOYBEAN CROPPING SYSTEM. [Internet] [Thesis]. University of New Hampshire; 2020. [cited 2021 Jan 23]. Available from: https://scholars.unh.edu/thesis/1392.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Palmer SA. PESTICIDE TREATED CROP SEEDS AND TILLAGE ALTER SEED COAT FUNGAL COMMUNITIES ON AMARANTHUS RETROFLEXUS IN A MAIZE-SOYBEAN CROPPING SYSTEM. [Thesis]. University of New Hampshire; 2020. Available from: https://scholars.unh.edu/thesis/1392

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

26. Leshinsky, Jana. Pharmacokinetics of Caspofungin Acetate in Healthy Adult Cats .

Degree: 2018, University of Sydney

URL: http://hdl.handle.net/2123/18159

► Background Sino-orbital aspergillosis (SOA) is an invasive fungal infection with high mortality due to difficulty in its treatment. SOA is commonly caused by cryptic species… (more)

▼ Background Sino-orbital aspergillosis (SOA) is an invasive fungal infection with high mortality due to difficulty in its treatment. SOA is commonly caused by cryptic species in Aspergillus section Fumigati that are often resistant to antifungal triazoles but susceptible to caspofungin. Caspofungin, an echinocandin, is indicated for the treatment of refractory invasive aspergillosis in humans and has been used successfully in cats with SOA. Pharmacokinetic data is lacking in cats. Aim To determine the pharmacokinetics of caspofungin acetate and determine optimal dosing for clinical use. Materials and Methods Eight adult healthy cats were administered caspofungin (1mg/kg IV) over 1hr (Day 1). Six cats subsequently received caspofungin daily for an additional 6 days. Blood was collected at various intervals after drug administration (Day 1), before the next dose (Days 2–7), and 24h after final dosing (Day 8). Plasma caspofungin levels were determined using HPLC-tandem mass spectrometry. Nonlinear mixed-effects pharmacokinetic modelling and simulation was used to investigate caspofungin population pharmacokinetics and explore dosing regimens in cats using caspofungin minimum effective concentrations (MECs). In the final pharmacokinetic model an optimum maximum concentration (Cmax):MEC ratio of 10-20 was used to guide caspofungin efficacy. Results Using a 1mg/kg dose Cmax was 14.8µg/mL, Cmax at steady state was 19.8µg/mL, Cmin was 5µg/mL and Cmax:MEC was >20 in 42.6% cats after multiple doses. An optimal Cmax:MEC ratio was achieved in caspofungin simulations using 0.75mg/kg q24h or 1mg/kg q72h. Conclusion Mean plasma caspofungin concentrations were >1.0g/ml for the duration of the sampling period, which exceeds the MIC effective against most Aspergillus species. Based on modelling data, a daily caspofungin dose of 0.75mg/kg q24h is likely to achieve target therapeutic concentrations, meet the proposed Cmax:MEC window and provide consistent exposure between doses.

Subjects/Keywords: Caspofungin; Aspergillosis; Cat; Fungal Rhinitis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leshinsky, J. (2018). Pharmacokinetics of Caspofungin Acetate in Healthy Adult Cats . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/18159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leshinsky, Jana. “Pharmacokinetics of Caspofungin Acetate in Healthy Adult Cats .” 2018. Thesis, University of Sydney. Accessed January 23, 2021. http://hdl.handle.net/2123/18159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leshinsky, Jana. “Pharmacokinetics of Caspofungin Acetate in Healthy Adult Cats .” 2018. Web. 23 Jan 2021.

Vancouver:

Leshinsky J. Pharmacokinetics of Caspofungin Acetate in Healthy Adult Cats . [Internet] [Thesis]. University of Sydney; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2123/18159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leshinsky J. Pharmacokinetics of Caspofungin Acetate in Healthy Adult Cats . [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/18159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

27. Zhang, Ning. Quantification of the proliferation of soil fungi in three dimensions using micro-computer aided tomography .

Degree: 2015, University of Sydney

URL: http://hdl.handle.net/2123/13691

► Good soil structure is important for sustaining agricultural production and preserving functions of the soil ecosystem. Soil aggregation is a critically important component of soil… (more)

▼ Good soil structure is important for sustaining agricultural production and preserving functions of the soil ecosystem. Soil aggregation is a critically important component of soil structure. Stable aggregates enable water infiltration, gas exchange for biological activities of plant roots and microorganisms, living space and surfaces for soil microbes, and contribute to stabilization of organic matter and storage of organic carbon (OC) in soil. Soil aggregation process involves fine roots, organic matter and fungal hyphae. Hyphal proliferation is essential for soil aggregation, and sequestration of OC in soil. Organic materials and available phosphorus are two of the major factors that influence fungi in soil. Organic materials are a source of energy for saprotrophic microbes and fungal hyphae may increase in the presence of organic matter. Phosphorus is an important element usually found depleted in soil ecosystems. The low availability of phosphorus may limit the biological activity of microbes. Arbuscular mycorrhizal fungi (AM) benefit plants by delivering phosphorus to the root system. However, the density and the length of hyphae of AM fungi do not appear to be increased by available phosphorus. We do not yet have a mechanism to directly quantify the density of hyphae in soil. A number of indirect methods have been used to visualize distribution of fungi in soil. Reliable analyses of soil are limited because of the use of destructive harvests to gain information. Soils are fragile, and fragility limits opportunity for non-destructive analysis. The soil ecosystem is also complex. Soil particles are dense and the density obscures the visualization of fungal hyphae. Fungal hyphae are relatively fine and information at the small scale (<250 µm) is key to understanding how fungi respond to environmental stimuli. The experiments conducted here developed a new method for the observation of fungi and quantification of hyphae in three dimensions. The methods were first tested using a melanised saprotrophic fungus, 222A. The response of two AM fungi, Glomus mosseae and Glomus pellucidum, growing together to common environmental factors was then quantified. Hyphae were quantified in an artificial soil matrix over 6 week’s incubation using micro-computer aided tomography (microCT). MicroCT provides three dimensional images of mycelia within electron lucent materials and enables the visualization and quantification of hyphae. Starch stimulated proliferation of 222A after 2 weeks. Starch, and starch and K2HPO4, stimulated proliferation of hyphae of AM fungi after 3 and 6 weeks. K2HPO4 stimulated hyphal proliferation only after 3 weeks. The images also indicate that fungal hyphae grew appressed to the surfaces of the particles rather than through the spaces between the particles. The capacity to quantify hyphae in three-dimensional space allows a wide range of questions to now be addressed. Apart from studying mechanisms of carbon turnover, more complex processes may now be considered. Soil is commonly thought of as a black…

Subjects/Keywords: microCT,; polystyrene; quantitative; fungal proliferation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, N. (2015). Quantification of the proliferation of soil fungi in three dimensions using micro-computer aided tomography . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/13691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Ning. “Quantification of the proliferation of soil fungi in three dimensions using micro-computer aided tomography .” 2015. Thesis, University of Sydney. Accessed January 23, 2021. http://hdl.handle.net/2123/13691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Ning. “Quantification of the proliferation of soil fungi in three dimensions using micro-computer aided tomography .” 2015. Web. 23 Jan 2021.

Vancouver:

Zhang N. Quantification of the proliferation of soil fungi in three dimensions using micro-computer aided tomography . [Internet] [Thesis]. University of Sydney; 2015. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2123/13691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang N. Quantification of the proliferation of soil fungi in three dimensions using micro-computer aided tomography . [Thesis]. University of Sydney; 2015. Available from: http://hdl.handle.net/2123/13691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cincinnati

28. Indugula, Reshmi. Fungal Exposure and Development of Autoimmune Disorders.

Degree: MS, Medicine: Immunology, 2017, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1505124409594398

► Over the past decade there has been an alarming increase in prevalence of autoimmune diseases worldwide. They occur as a result of body’s inability to… (more)

Subjects/Keywords: Immunology; Fungal exposure; Autoimmune disorders

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Indugula, R. (2017). Fungal Exposure and Development of Autoimmune Disorders. (Masters Thesis). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1505124409594398

Chicago Manual of Style (16^th Edition):

Indugula, Reshmi. “Fungal Exposure and Development of Autoimmune Disorders.” 2017. Masters Thesis, University of Cincinnati. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1505124409594398.

MLA Handbook (7^th Edition):

Indugula, Reshmi. “Fungal Exposure and Development of Autoimmune Disorders.” 2017. Web. 23 Jan 2021.

Vancouver:

Indugula R. Fungal Exposure and Development of Autoimmune Disorders. [Internet] [Masters thesis]. University of Cincinnati; 2017. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1505124409594398.

Council of Science Editors:

Indugula R. Fungal Exposure and Development of Autoimmune Disorders. [Masters Thesis]. University of Cincinnati; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1505124409594398

29. Prasad, Kalyani. Transgenic groundnut with resistance to fungal pathogens (Foliar diseases of Groundnut); -.

Degree: Biotechnology, 2011, Jawaharlal Nehru Technological University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/4511

►

The late leaf spot (LLS) disease caused by Phaeoisariopsis personata (Berk. And Curt.) Van Arx, and rust caused by Puccinia arachidis Speg. are economically important… (more)

▼

The late leaf spot (LLS) disease caused by Phaeoisariopsis personata (Berk. And Curt.) Van Arx, and rust caused by Puccinia arachidis Speg. are economically important foliar fungal diseases of groundnut (Arachis hypogaea L.). The annual losses caused by LLS and rust are over US 599 m and US 467 m, respectively. The development of groundnut germplasm with durable resistance to these fungal pathogens would provide the most effective and viable option for crop improvement. Here we report enhanced fungal resistance in groundnut by introducing a constitutively expressed rice chitinase gene (Rchit) with anti-fungal properties that have been shown to play significant role in plant defense by degrading the chitin of fungal cell walls. Over 75 independently transformed transgenic events were developed with the popular groundnut varieties JL 24, ICGV 89104 and ICGV 86031 through Agrobacterium -mediated genetic transformation by using the cotyledon explants from mature imbibed seeds. The transgenic plants were characterized at the molecular level for gene integration, copy number and expression of the transgene. Several transgenic events showed about 2- to 14-fold higher level of chitinase activity than the untransformed controls. The transgenic events showing high level of chitinase activity were selected for in vitro seed colonization with A. flavus. Seeds of transgenic events showing 0-10% A. flavus infection were advanced to subsequent generations and fungal bioassays for late leaf spot and rust were carried out using detached leaf technique. Results revealed an increased incubation period and latent period and a lower infection frequency (50-100% reduction in disease incidence) indicating a varying level of resistance in the selected transgenic plants as compare to the untransformed controls. The selected transgenic events thus identified will be used for further field evaluations and if confirmed, as resistance sources to develop groundnut varieties with enhanced resistance to the foliar fungal pathogens and A.

Summary and Conclusions p.132-137, Bibliography p.138-188, Annexture p.189-199

Advisors/Committee Members: Waliyar, Farid, Narasu, Lakshmi M.

Subjects/Keywords: stresses; Foliar fungal diseases; Late leaf spot; fungal disease; Biotechnology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prasad, K. (2011). Transgenic groundnut with resistance to fungal pathogens (Foliar diseases of Groundnut); -. (Thesis). Jawaharlal Nehru Technological University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/4511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prasad, Kalyani. “Transgenic groundnut with resistance to fungal pathogens (Foliar diseases of Groundnut); -.” 2011. Thesis, Jawaharlal Nehru Technological University. Accessed January 23, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/4511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prasad, Kalyani. “Transgenic groundnut with resistance to fungal pathogens (Foliar diseases of Groundnut); -.” 2011. Web. 23 Jan 2021.

Vancouver:

Prasad K. Transgenic groundnut with resistance to fungal pathogens (Foliar diseases of Groundnut); -. [Internet] [Thesis]. Jawaharlal Nehru Technological University; 2011. [cited 2021 Jan 23]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prasad K. Transgenic groundnut with resistance to fungal pathogens (Foliar diseases of Groundnut); -. [Thesis]. Jawaharlal Nehru Technological University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

30. Zhou, Mian. The Role of Codon Usage in Regulating Protein Expression, Structure and Function.

Degree: 2014, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/3573

► Codon usage bias has been observed in the genomes of almost all organisms and is thought to result from selection for efficient translation of highly… (more)

▼ Codon usage bias has been observed in the genomes of almost all organisms and is thought to result from selection for efficient translation of highly expressed genes. Many genes, however, exhibit little codon usage bias. It's not clear whether the lack of codon bias for a gene is due to lack of selection for mRNA translation or it has some biological significance. The rhythmic expression and the proper function of the Neurospora FREQUENCY (FRQ) protein are essential for circadian clock function. However, unlike most genes inNeurospora, frq exhibits non-optimal codon usage across its entire open reading frame (ORF). Optimization of frq codon usage results in the abolition of both overt and molecular circadian rhythms. Codon optimization not only increases FRQ expression level but surprisingly, also results in conformational changes in FRQ protein, impaired FRQ phosphorylation, and impaired functions in the circadian feedback loops. These results indicate that non-optimal codon usage of frq is essential for maintaining circadian rhythmicity in Neurospora. Interestingly, there is a correlation between codon usage score and FRQ protein structure: the regions that are predicted to be disordered preferentially uses more non-optimal codons. This negative correlation is also found in the proteasome of Neurospora, as well in yeast, Drosophila, C. elegans and E. coli. By making a series of Neurospora strains with frq optimized in different regions, we find that codon optimizations in the predicted disordered regions of FRQ have more prominent effects on FRQ activity and structure. Furthermore, codon optimization of disordered regions in several other Neurospora genes results in altered protein degradation rates, suggesting structural changes by codon optimization. Together, these results suggest that codon usage adapts to protein structures and there is a "code" within genetic codons that allow optimal co-translational protein folding. Advisors/Committee Members: Tu, Benjamin, Liu, Yi, Takahashi, Joseph, Zinn, Andrew R..

Subjects/Keywords: CLOCK Proteins; Codon; Fungal Proteins; Gene Expression Regulation, Fungal; Neurospora crassa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, M. (2014). The Role of Codon Usage in Regulating Protein Expression, Structure and Function. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/3573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Mian. “The Role of Codon Usage in Regulating Protein Expression, Structure and Function.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed January 23, 2021. http://hdl.handle.net/2152.5/3573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Mian. “The Role of Codon Usage in Regulating Protein Expression, Structure and Function.” 2014. Web. 23 Jan 2021.

Vancouver:

Zhou M. The Role of Codon Usage in Regulating Protein Expression, Structure and Function. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2152.5/3573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou M. The Role of Codon Usage in Regulating Protein Expression, Structure and Function. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/3573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations