subject:(Food)

Drexel University

1. Shah, Urvi Bharat. Effect of cold plasma treatment on microbial load, color and cuticle in baby kale (Brassica oleracea).

Degree: 2017, Drexel University

URL: http://hdl.handle.net/1860/idea:7404

► Kale leaves are consumed worldwide because of their promoting health and nutritional benefits. However, in the past two decades, there has been an increase in… (more)

▼ Kale leaves are consumed worldwide because of their promoting health and nutritional benefits. However, in the past two decades, there has been an increase in foodborne diseases outbreaks caused by microorganisms associated with leafy vegetables. Leafy vegetables are majorly consumed raw or are minimally processed, increasing the risk of pathogenic entry. The food industry faces challenges in improving food safety while maintaining quality of the food. Chemicals in water wash and heat treatments (70 °C to 121 °C) are the most common industrial methods of killing microorganisms which have negative effect on the aesthetic quality (color) and nutritional properties of the product respectively. Cold plasma treatment of fresh produce, an emerging food preservation process, is a fast and environmental- friendly process that disinfects the product at a low temperature (30-40 °C). It uses an ionized gas comprised of charged ions and free electrons for treatment of the product. Cold plasma kills microorganisms by altering their metabolic pathways. This suggests that this technology may also modify chemical and physical surface properties of food product. Till date, very little research has been focused on the effect of cold plasma on the quality and shelf life of fresh produce. The purpose of this study was to evaluate the efficacy of cold plasma technology in disinfecting baby kale leaves and to study its effect on color and cuticle composition. Cuticle is the extracellular wax present on the surface of leaves and forms a protective barrier between product surface and environment. Baby kale leaf samples were treated with cold plasma system for different treatment times and color and microbial load was evaluated at each treatment time. Untreated leaves and leaves treated with tap water mist were used as control. Cuticle analysis was conducted for samples treated with plasma mist at 240s. This research demonstrated the ability of the cold plasma system in complete inactivation of E. coli O157:H7 when baby kale leaves were treated with plasma for 300s. The number of viable microorganisms (E. coli) in baby kale leaves reduced from 3.3x102 ± 330 CFU/mL to 0.0 ± 0.0 CFU/mL when the plasma treatment time was increased from 60s to 300s respectively. While no significant changes in color values were observed when baby kale leaves were treated with plasma for 300s, leaf damage and extreme browning was observed in leaves treated with plasma for 600s. Tap water was used as a control for plasma treatment. At each treatment time, lower microbial load was observed for plasma treated leaves compared to tap water treated leaves. Further, tap water treated leaves at 300s and untreated leaves showed extremely high browning index of 109.0 ± 8.0 and 80.1 ± 6.2 respectively, after 12 days of storage in refrigerator compared to plasma treated leaves. On the other hand, plasma treatment enhanced color stability of the leaves during storage and their browning index value did not exceed 62.7 ± 7.1. Plasma treatment of 240s resulted in cuticular changes… Advisors/Committee Members: Sekhon, Jasreen K., , Center for Hospitality and Sport Management.

Subjects/Keywords: Food

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APA (6^th Edition):

Shah, U. B. (2017). Effect of cold plasma treatment on microbial load, color and cuticle in baby kale (Brassica oleracea). (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/idea:7404

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shah, Urvi Bharat. “Effect of cold plasma treatment on microbial load, color and cuticle in baby kale (Brassica oleracea).” 2017. Thesis, Drexel University. Accessed February 28, 2020. http://hdl.handle.net/1860/idea:7404.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shah, Urvi Bharat. “Effect of cold plasma treatment on microbial load, color and cuticle in baby kale (Brassica oleracea).” 2017. Web. 28 Feb 2020.

Vancouver:

Shah UB. Effect of cold plasma treatment on microbial load, color and cuticle in baby kale (Brassica oleracea). [Internet] [Thesis]. Drexel University; 2017. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/1860/idea:7404.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shah UB. Effect of cold plasma treatment on microbial load, color and cuticle in baby kale (Brassica oleracea). [Thesis]. Drexel University; 2017. Available from: http://hdl.handle.net/1860/idea:7404

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida State University

2. Jiang, Xingyi. Immunodetection of Porcine Blood in Foods.

Degree: MS, Nutrition, Food, and Exercise Science, 2017, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_2017SP_Jiang_fsu_0071N_13789 ;

► Justification: Different porcine blood proteins have been widely used as emulsifier, binder and/or colorant in processed foods. However, misusage of porcine blood ingredients, such as… (more)

▼ Justification: Different porcine blood proteins have been widely used as emulsifier, binder and/or colorant in processed foods. However, misusage of porcine blood ingredients, such as mislabeling and substitution, can cause religious objections, law violation, food safety and/or food quality decrease. These issues highlight the need of detecting unfavorable porcine blood in foods to fight food fraud. Porcine whole blood, plasma and blood cells can be applied individually or in combination as food additives. Therefore, the study was divided into two parts. The objectives of the part 1 is (1) to develop and characterize a monoclonal antibody (mAb) that is specific for porcine hemoglobin; (2) to develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) that can detect porcine blood adulteration in foods. The objective of part Ⅱ is to characterize two mAbs which have the target protein in porcine plasma. Methods: In part Ⅰ, mAbs were developed using hybridoma technique and purified using immunoaffinity. Western blot was applied to verify the target protein; to study the mAb selectivity; and to study the effect of pH on storage stability (29 days at 4 °C) and thermostability (50 ºC, 100 °C and 121 ºC for 15 min) of target protein. Indirect non-competitive ELISA (inELISA) was performed to study antibody affinity and storage stability of target protein, and to choose the optimized condition for icELISA. Eventually, an optimized icELISA and extraction buffer was developed. The assay was validated by FDA Guidance for Industry. In part Ⅱ, immunoaffinity column was applied to isolate the target protein. The isolated proteins were sequenced. The immunoreactivity of target protein was verified using four commercial antibodies (anti-transferrin, anti-haptoglobin, anti-plasminogen and anti-C7). To further investigate the isoelectric point (pI) and disulfide information of target protein, two-dimensional gel electrophoresis and non-reducing SDS-PAGE were performed, respectively. Results: In part Ⅰ, mAb13F7 was chosen after screening test because it has the best selectivity to porcine blood. The target protein of the mAb was porcine hemoglobin (PHb) subunit (14 kDa). Although this mAb could cross-react with hemoglobin from bovine, horse and sheep, their hemoglobin band color intensity was much less than that of PHb according to Western blot. From inELISA and icELISA, this mAb showed a high immunoaffinity to PHb compared with bovine hemoglobin. The affinity constant of this mAb is in a nanomolar range, which can be considered as high-affinity antibody. As for thermostability, PHb can maintain the best molecular integrity and immunoreactivity at alkaline pH compared to acidic pH and neutral pH. During storage at 4ºC up to one month, PHb remained intact without any degraded peptides observed and the immunoreactivity did not change significantly (P > 0.05). Finally, a sample extraction buffer (12.5 mM NaHCO3 and 25 Mm NaCl) and an anti-PHb cELISA were developed. After assay validation, the optimized cELISA was… Advisors/Committee Members: Qinchun Rao (professor directing thesis), Shridhar K. Sathe (committee member), Timothy M. Logan (committee member).

Subjects/Keywords: Food

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APA (6^th Edition):

Jiang, X. (2017). Immunodetection of Porcine Blood in Foods. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_2017SP_Jiang_fsu_0071N_13789 ;

Chicago Manual of Style (16^th Edition):

Jiang, Xingyi. “Immunodetection of Porcine Blood in Foods.” 2017. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_2017SP_Jiang_fsu_0071N_13789 ;.

MLA Handbook (7^th Edition):

Jiang, Xingyi. “Immunodetection of Porcine Blood in Foods.” 2017. Web. 28 Feb 2020.

Vancouver:

Jiang X. Immunodetection of Porcine Blood in Foods. [Internet] [Masters thesis]. Florida State University; 2017. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_2017SP_Jiang_fsu_0071N_13789 ;.

Council of Science Editors:

Jiang X. Immunodetection of Porcine Blood in Foods. [Masters Thesis]. Florida State University; 2017. Available from: http://purl.flvc.org/fsu/fd/FSU_2017SP_Jiang_fsu_0071N_13789 ;

Florida State University

3. Undem, Miranda Kaye. Factors Affecting the Ability to Sustain Maximal Work.

Degree: MS, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1458 ;

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Purpose: The purpose of this investigation was to examine the ability to sustain a maximal running effort. Furthermore, the effects of aerobic fitness level, gender,… (more)

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Purpose: The purpose of this investigation was to examine the ability to sustain a maximal running effort. Furthermore, the effects of aerobic fitness level, gender, anaerobic capacity, efficiency of energy production and utilization, and lactate threshold on maximal performance were examined. Methods: Maximal oxygen consumption (VO2max), ventilatory threshold (VT) and anaerobic capacity (AC) were determined for 22 subjects. Subjects then performed a treadmill test to exhaustion to determine the time spent at VO2max, time spent at the velocity (run speed) associated with 100% VO2max (vVO2max), and the time it took to reach VO2max at vVO2max. Statistical analysis included one-way ANOVA by gender, VO2max, gender/VO2max and VO2max/AC. Tukey's post-hoc tests were done to determine location of significance. Pearson product-moment correlations were performed between physiologic variables [VO2max, AC, VT, gross efficiency (GE) and net efficiency (NE)] and maximal run performance (time spent at VO2max and vVO2max, and time to reach VO2max). Results: Average time spent at VO2max was 0.79±0.13 minutes and time spent at vVO2max was 2.36±0.20 minutes. No differences in maximal run performance were observed between high fit (HF) and low fit (LF) groups; however, females spent significantly longer time at VO2max and vVO2max compared with males (p Conclusions: VO2max does not appear to be directly related with the ability to sustain a maximal run performance, while females spent a longer time at VO2max and vVO2max than males. GE and NE showed a moderately positive relationship with time at vVO2max and time to reach VO2max.

A Thesis Submitted to the Department of Nutrition, Food & Exercise Sciences in Partial Fulfillment of the Requirements for the Degree of Master of Science.

Summer Semester, 2010.

June 30, 2010.

Gross and Net Efficiency, Anaerobic Capacity, Ventilatory Threshold, Time to Reach VO2max, vVO2max, VO2max

Robert J. Moffatt, Professor Directing Thesis; Lynn B. Panton, Committee Member; Thomas Ratliffe, Committee Member.

Advisors/Committee Members: Robert J. Moffatt (professor directing thesis), Lynn B. Panton (committee member), Thomas Ratliffe (committee member).

Subjects/Keywords: Food

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APA (6^th Edition):

Undem, M. K. (2010). Factors Affecting the Ability to Sustain Maximal Work. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-1458 ;

Chicago Manual of Style (16^th Edition):

Undem, Miranda Kaye. “Factors Affecting the Ability to Sustain Maximal Work.” 2010. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-1458 ;.

MLA Handbook (7^th Edition):

Undem, Miranda Kaye. “Factors Affecting the Ability to Sustain Maximal Work.” 2010. Web. 28 Feb 2020.

Vancouver:

Undem MK. Factors Affecting the Ability to Sustain Maximal Work. [Internet] [Masters thesis]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1458 ;.

Council of Science Editors:

Undem MK. Factors Affecting the Ability to Sustain Maximal Work. [Masters Thesis]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1458 ;

Florida State University

4. Schaefer, Julie. Disordered Eating, Body Image, and Healthy Weight Maintenance: Follow Up Study.

Degree: MS, Nutrition, Food, and Exercise Science, 2011, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2060 ;

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Objective: The objective of this research was to investigate the sustainability of the effects of an eating disorder prevention curriculum on college women. Participants: Participants… (more)

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schaefer, J. (2011). Disordered Eating, Body Image, and Healthy Weight Maintenance: Follow Up Study. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-2060 ;

Chicago Manual of Style (16^th Edition):

Schaefer, Julie. “Disordered Eating, Body Image, and Healthy Weight Maintenance: Follow Up Study.” 2011. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-2060 ;.

MLA Handbook (7^th Edition):

Schaefer, Julie. “Disordered Eating, Body Image, and Healthy Weight Maintenance: Follow Up Study.” 2011. Web. 28 Feb 2020.

Vancouver:

Schaefer J. Disordered Eating, Body Image, and Healthy Weight Maintenance: Follow Up Study. [Internet] [Masters thesis]. Florida State University; 2011. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2060 ;.

Council of Science Editors:

Schaefer J. Disordered Eating, Body Image, and Healthy Weight Maintenance: Follow Up Study. [Masters Thesis]. Florida State University; 2011. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2060 ;

Florida State University

5. Muravyeva, Yelizaveta. Does Ferutinin Dose-Dependently Increase Nodule Formation in TNF-Alpha Activated MC3T3-E1 Preosteoblast-like Cells?.

Degree: MS, Nutrition, Food, and Exercise Science, 2011, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2185 ;

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Forty-four million men and women are affected by osteoporosis in the United States. Women are at a greater risk for developing osteoporosis due to their… (more)

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Forty-four million men and women are affected by osteoporosis in the United States. Women are at a greater risk for developing osteoporosis due to their lower peak bone mass and going through menopause which accelerates bone loss (1). Treatments for osteoporosis may be associated with adverse effects such as renal toxicity and osteonecrosis of the jaw (2). Contrary to earlier findings that hormone replacement therapy (HRT) decreases risk factors for cardiovascular disease, the Women's Health Initiative Study revealed that HRT actually increases risk factors for cardiovascular disease such as high blood pressure and thrombosis. These findings along with increases in incidence of endometrial and breast cancer, have made women seek alternative therapies (3). Among dietary alternatives, ferutinin, a phytochemical from the genus Ferula Hermonis (Umbelliferae), has recently been reported to have the benefits of estrogen without any side effects. Ferutinin is grown in Syria, Lebanon, and Jordan. It plays a role in alleviating different health problems and recent findings suggest that this plant extract can prevent bone loss and excess body weight gain in ovariectomized rats. Although there has been research done with ferutinin and bone in animal models, the mechanism by which ferutinin exerts its bone protective effects is unknown. Therefore, we identified what effects ferutinin had on tumor necrosis factor- α (TNF-α) activated MC3T3-E1 preosteoblast-like cells. The experiment involved treating the cells using TNF-α to induce inflammation that is associated with osteoporosis. Alkaline phosphatase activity, nodule formation, and cell viability were measured to evaluate the effect of ferutinin on preosteoblast- like cells. Unlike previous studies with ferutinin, the findings of this study suggest that ferutinin does not modulate bone parameters in preosteoblast-like cells.

A Thesis Submitted to the Department of Nutrition, Food, and Exercise Sciences in Partial Fulfillment of the Requirements for the Degree of Master of Science.

Summer Semester, 2011.

May 4, 2011.

ferutinin, steoporosis, phytochemical, cell culture, bone, alternative therapy, preosteoblasts

Bahram H. Arjmandi, Professor Directing Thesis; Lisa Eckel, Committee Member; Jeong-Su Kim, Committee Member.

Advisors/Committee Members: Bahram H. Arjmandi (professor directing thesis), Lisa Eckel (committee member), Jeong-Su Kim (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Muravyeva, Y. (2011). Does Ferutinin Dose-Dependently Increase Nodule Formation in TNF-Alpha Activated MC3T3-E1 Preosteoblast-like Cells?. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-2185 ;

Chicago Manual of Style (16^th Edition):

Muravyeva, Yelizaveta. “Does Ferutinin Dose-Dependently Increase Nodule Formation in TNF-Alpha Activated MC3T3-E1 Preosteoblast-like Cells?.” 2011. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-2185 ;.

MLA Handbook (7^th Edition):

Muravyeva, Yelizaveta. “Does Ferutinin Dose-Dependently Increase Nodule Formation in TNF-Alpha Activated MC3T3-E1 Preosteoblast-like Cells?.” 2011. Web. 28 Feb 2020.

Vancouver:

Muravyeva Y. Does Ferutinin Dose-Dependently Increase Nodule Formation in TNF-Alpha Activated MC3T3-E1 Preosteoblast-like Cells?. [Internet] [Masters thesis]. Florida State University; 2011. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2185 ;.

Council of Science Editors:

Muravyeva Y. Does Ferutinin Dose-Dependently Increase Nodule Formation in TNF-Alpha Activated MC3T3-E1 Preosteoblast-like Cells?. [Masters Thesis]. Florida State University; 2011. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2185 ;

Florida State University

6. Wentz, Laurel McLean. Calcium Intake and the Incidence of Stress Fractures.

Degree: PhD, Nutrition, Food, and Exercise Science, 2011, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1183 ;

► Purpose: The purpose of this study was to compare female runners with a history of stress fractures to matched runners who have no history of… (more)

▼ Purpose: The purpose of this study was to compare female runners with a history of stress fractures to matched runners who have no history of stress fractures to determine if differences existed in adolescent dairy intake, current dietary calcium intake, total energy, bone mineral density, and menstrual irregularities. Methods: 27 female runners (aged 18-40) who had at least one stress fracture that had been diagnosed by a doctor were matched to a control sample of 32 female runners who were similar in age, weight, and fat free mass (FFM). Subjects visited the lab in the late follicular phase of their menstrual cycle and consented to a fasting blood draw for assessment of serum estradiol, serum intact parathyroid hormone (iPTH), and serum 25-hydroxyvitamin D (25-(OH)D). Bone measurements were performed by dual-energy x-ray absorptionmetry (DXA). Subjects answered a questionnaire on stress fracture history, training, menstrual status, and lifetime dairy intake, and they also completed a 3-day food record. Results: Subjects did not differ in age, weight, height, FFM, body mass index, or training volume. Menstrual history and current menstrual characteristics were not significantly different between groups, nor were bone measurements at any sites. A greater number of stress fracture subjects were using calcium and/or vitamin D supplements compared to control subjects (p<0.05), and there was a trend for greater dietary calcium intake in control subjects (p=0.077). Previous and current servings of dairy intake did not differ between groups. However, in multiple linear regression, weekly servings of milk during middle school were a significant predictor (p<0.05) of BMD in the total femur, femoral neck, Ward's triangle, and femoral shaft. Number of menstrual cycles in the previous year was a significant positive predictor of femoral neck BMD (p<0.05). Caffeine intake was significantly negatively correlated with BMD of the total femur, femoral neck, trochanter, and femoral shaft. Some training variables were significantly negatively associated with bone, including running pace and total femur, days run per week and 33% radius, and also hours spent exercising per week were negatively correlated with total body BMD, total body BMC, and lumbar spine BMD. Logistic regression was used to identify risk factors for stress fractures, from which two models were created. The first model included the number of years subjects had been running, regular or irregular menstrual history, total body BMD, current dietary calcium, serum 25-(OH)D, and body mass index. The second model included the percent of time subjects trained on hard ground, current dietary calcium, regular or irregular menstrual history, total body BMD, serum 25-(OH)D, and body mass index . Conclusions: Although no significant differences in BMD of various skeletal sites emerged between female runners with and without stress fractures, higher dairy intake during adolescent years, especially middle school milk servings, was highly positively correlated with BMD.… Advisors/Committee Members: Emily Haymes (professor directing dissertation), Penny J. Gilmer (university representative), Jasminka Z. Ilich-Ernst (committee member).

Subjects/Keywords: Food

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APA (6^th Edition):

Wentz, L. M. (2011). Calcium Intake and the Incidence of Stress Fractures. (Doctoral Dissertation). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-1183 ;

Chicago Manual of Style (16^th Edition):

Wentz, Laurel McLean. “Calcium Intake and the Incidence of Stress Fractures.” 2011. Doctoral Dissertation, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-1183 ;.

MLA Handbook (7^th Edition):

Wentz, Laurel McLean. “Calcium Intake and the Incidence of Stress Fractures.” 2011. Web. 28 Feb 2020.

Vancouver:

Wentz LM. Calcium Intake and the Incidence of Stress Fractures. [Internet] [Doctoral dissertation]. Florida State University; 2011. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1183 ;.

Council of Science Editors:

Wentz LM. Calcium Intake and the Incidence of Stress Fractures. [Doctoral Dissertation]. Florida State University; 2011. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1183 ;

Florida State University

7. Wilson, Jacob M. (Jacob Malachi). Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Body Composition, Functionality, MR-Determined Myofiber Dimensions, and Transcript Factors Inovolved in Myofiber Growth in Young and Old Fisher 344 Rats.

Degree: PhD, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-0903 ;

► 'Sarcopenic obesity,' a recent medical term, refers to a new trend in aged individuals who simultaneously demonstrate reductions and increases in lean mass and fat… (more)

▼ 'Sarcopenic obesity,' a recent medical term, refers to a new trend in aged individuals who simultaneously demonstrate reductions and increases in lean mass and fat mass, respectively. As the elderly population increases, the economic burden of sarcopenia also tremendously increases. Recent evidence reveals that HMB directly increases fat oxidation and regulates myoblast differentiation and survival in cell culture, supporting a possible role in attenuating sarcopenic obesity. However, the efficacy of HMB on myogenic capacity and myofiber dimensions has not been investigated. Therefore, the overarching aim of the present study was to determine the effects of 16 wks of HMB administration in young and old rats on age-related changes in body composition, functionality and myofiber dimensions. We also sought to investigate the possible cellular and molecular mechanisms, which mediated these effects. Twelve young (44 wks), 6 middle aged (60 wks), 10 old (86 wks), and 5 very old (102 wks) male Fisher 344 rats were studied. Their body compositions (dual X-ray absorptiometry, DXA), grip strength and sensory motor function (incline plane) were assessed pre- and post-intervention. After DXA, 6 young, 6 middle aged, 5 old and very old rats were sacrificed for baseline muscles, and remaining young (44 wks) and old (86 wks) rats were given 1% HMB (0.46 g/kg/d) feed for 16 wks and then sacrificed for pre- and post-HMB molecular analysis and for diffusion tensor imaging (DTI) of the soleus and gastrocnemius muscles. Transcript factors involved in muscle cell regeneration and growth: myogenin, myogenic differentiation factor (MyoD), and insulin-like growth factor-IEa (IGF-IEa), mechano growth factor (MGF), myostatin, eukaryotic initiation factor 4E binding protein 1 (4EBP1), and atrogin-1 were also assessed with RT-PCR. There was a group effect for total body mass (TBM) increasing (+23%) in the control, but not in the HMB group from 44-60 wks, while it maintained in the control, and declined (-20%) in the HMB group from 86-102 wks. Fat mass (g) increased in the middle aged control (+49%) but not in the HMB group. Fat mass declined (-56%) in the old HMB group but not in the control group. There were overall declines of 25% in absolute grip strength from 44 to 102 wks of age. However, when normalized to TBM, there was a significant group x time effect (p<0.05) in which strength declined in the control group, but was maintained in the HMB group from 44-60 wks. Moreover, HMB improved strength normalized to TBM (+23%) (p<0.05) from 86-102 wks, with no change in the control group. There was a main time effect for sensory motor function, declining 14% (p<0.05) from 44 to 102 wks of age. However, the HMB group demonstrated an 11% increase (p<0.01) from 44-60 wks. There were declines in gastrocnemius, plantaris, and soleus weights normalized to TBM from 60-102 wks. While HMB had no effects on the gastrocnemius, there were decreases in control planaris (-21%) and soleus (-15%) muscles, which maintained and increased (+12%), respectively, in… Advisors/Committee Members: Jeong-Su Kim (professor directing dissertation), Samuel Grant (university representative), Lynn B. Panton (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wilson, J. M. (. M. (2010). Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Body Composition, Functionality, MR-Determined Myofiber Dimensions, and Transcript Factors Inovolved in Myofiber Growth in Young and Old Fisher 344 Rats. (Doctoral Dissertation). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-0903 ;

Chicago Manual of Style (16^th Edition):

Wilson, Jacob M (Jacob Malachi). “Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Body Composition, Functionality, MR-Determined Myofiber Dimensions, and Transcript Factors Inovolved in Myofiber Growth in Young and Old Fisher 344 Rats.” 2010. Doctoral Dissertation, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-0903 ;.

MLA Handbook (7^th Edition):

Wilson, Jacob M (Jacob Malachi). “Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Body Composition, Functionality, MR-Determined Myofiber Dimensions, and Transcript Factors Inovolved in Myofiber Growth in Young and Old Fisher 344 Rats.” 2010. Web. 28 Feb 2020.

Vancouver:

Wilson JM(M. Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Body Composition, Functionality, MR-Determined Myofiber Dimensions, and Transcript Factors Inovolved in Myofiber Growth in Young and Old Fisher 344 Rats. [Internet] [Doctoral dissertation]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-0903 ;.

Council of Science Editors:

Wilson JM(M. Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Body Composition, Functionality, MR-Determined Myofiber Dimensions, and Transcript Factors Inovolved in Myofiber Growth in Young and Old Fisher 344 Rats. [Doctoral Dissertation]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-0903 ;

Florida State University

8. Shin, Hyehyung. Physical Performance during 6- and 12-Month Weight Loss in Postmenopausal Women: Relationship with Body Composition and Bone Mineral Density.

Degree: PhD, Nutrition, Food, and Exercise Science, 2011, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1749 ;

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The overall purpose of this study is to investigate the physical performance of early postmenopausal Caucasian women during a 12-month weight reduction program. Additionally, the… (more)

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The overall purpose of this study is to investigate the physical performance of early postmenopausal Caucasian women during a 12-month weight reduction program. Additionally, the relationships between body composition (bone, fat and lean mass) and physical performance during that period is explored. A total of 97 participants aged 56.0(±4.3) years old with an average BMI of 30.3(±3.8) kg/m2 were included in this study. Of those, 66 (68 %) and 55 (57 %) participants completed the 6-month and 12-month interventions, respectively. The participants followed prescribed energy-restricted meal plans and were divided into three groups based on calcium and vitamin D intake: hypocaloric diet with calcium+vitamin D supplements (1260 mg/day+800 IU/day), hypocaloric diet with placebo or hypocaloric diet with 5-6 servings of low-fat dairy products. Physical performance measures including handgrip strength, 8-meter walking speed, one leg stance (OLS), timed 8-foot get-up and go test (TGUG) and chair sit-to-stand test (STS) and iDXA scan for body composition and bone mineral density (BMD) were performed at baseline, month 6 and month 12. Fasting blood were drawn to analyze the concentration of serum 25 hydroxyvitamin D (25OHD) by using ELISA. At baseline, a higher percentage of body fat was correlated with poorer physical performance on non-dominant OLS, TGUG, walking speed and STS. Multiple regression analyses controlling for age, weight, height, calcium intake, serum 25OHD, physical activity and smoking status revealed that having more leg fat (%) was related to poorer performance on OLS, TGUG, walking speed and STS indicating this relationship was site specific. In addition, handgrip strength was positively related to total lean mass. Only forearm BMD (radius 33%) was positively correlated with handgrip strength among various skeletal sites. During the 12-month intervention, participants decreased in weight from 81.4±12.1 kg (BMI of 30.2±3.7 kg/m2) to 78.4±13.4 kg (BMI of 29.1±4.3 kg/m2, p<0.001). Among physical performance measures, walking speed and chair STS were significantly improved. The results of linear mixed models showed that the decrease in weight and percentage of fat were related to the improvement in all physical performance measures over time. The change in the amount of lean mass resulted in a positive relationship with the change in the handgrip strength, OLS, walking speed and STS over time. However, no significant relationship was observed between BMD and physical performance measures. Overall, the percentage of fat and its change with weight loss were the most significant predictors of physical performance and its change in overweight/obese postmenopausal women. Even the modest weight loss resulted in improvement of some of the physical performance measures in this cohort of overweight/obese early postmenopausal women.

A Dissertation submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

Spring…

Advisors/Committee Members: Jasminka Ilich-Ernst (professor directing dissertation), Gareth R. Dutton (university representative), Lynn B. Panton (committee member), Maria Spicer (committee member).

Subjects/Keywords: Food

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APA (6^th Edition):

Shin, H. (2011). Physical Performance during 6- and 12-Month Weight Loss in Postmenopausal Women: Relationship with Body Composition and Bone Mineral Density. (Doctoral Dissertation). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-1749 ;

Chicago Manual of Style (16^th Edition):

Shin, Hyehyung. “Physical Performance during 6- and 12-Month Weight Loss in Postmenopausal Women: Relationship with Body Composition and Bone Mineral Density.” 2011. Doctoral Dissertation, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-1749 ;.

MLA Handbook (7^th Edition):

Shin, Hyehyung. “Physical Performance during 6- and 12-Month Weight Loss in Postmenopausal Women: Relationship with Body Composition and Bone Mineral Density.” 2011. Web. 28 Feb 2020.

Vancouver:

Shin H. Physical Performance during 6- and 12-Month Weight Loss in Postmenopausal Women: Relationship with Body Composition and Bone Mineral Density. [Internet] [Doctoral dissertation]. Florida State University; 2011. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1749 ;.

Council of Science Editors:

Shin H. Physical Performance during 6- and 12-Month Weight Loss in Postmenopausal Women: Relationship with Body Composition and Bone Mineral Density. [Doctoral Dissertation]. Florida State University; 2011. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1749 ;

Florida State University

9. Saadat, Raz L. The Efficacy of Dried Plum in Modulting Biomarkers of Bone Turnover in Postmenopausal Women.

Degree: MS, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2143 ;

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Previous studies from our lab suggest that dried plum may exert positive effects on biomarkers of bone turnover in animal studies and a short-term clinical… (more)

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Previous studies from our lab suggest that dried plum may exert positive effects on biomarkers of bone turnover in animal studies and a short-term clinical trial. Dried plum is not only a rich source of vitamins, minerals and polyphenols, but has been reported as having high antioxidant properties that may be responsible in prevention of bone loss. The purpose of this study was to examine the effect of three-month dried plum consumption on the biomarkers of bone turnover in postmenopausal women. A total of 123 postmenopausal women experiencing mild bone loss and who were free of hormone replacement therapy were randomly assigned to consume either 100 grams of dried plum or a nutritional equivalence of dried apple for three consecutive months. Bone-specific alkaline phosphatase (B-SAP), osteocalcin (OC), tartrate-resistant acid phosphatase-5b (TRAP5b), and deoxypyridinoline (Dpd) were analyzed at baseline and after three months to test the effect of daily consumption of dried plum on bone turnover markers. Repeated measures (RM) multiple analysis of variance (ANOVA) followed by RM ANOVA were conducted to analyze the results. Findings of this study indicated no significant differences between the dried plum and dried apple groups when the data were analyzed considering treatment by time interaction. However, treatment by dried plum increased Dpd, while time decreased TRAP5b and increased OC in the dried plum group. The inconsistency between markers of resorption, Dpd and TRAP5b, as well as discrepancies between our findings and previous studies suggests that blood and serum markers cannot be used to predict the effect of dried plum on bone density unless bone mineral density (BMD) is measured as the end point variable that would require study duration of at least six months and preferably longer.

A Thesis submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Master of Science.

Spring Semester, 2010.

March 5, 2010.

Dried Plum, Bone, Women, Osteoporosis

Bahram H. Arjmandi, Professor Directing Thesis; Maria Spicer, Committee Member; Gershon Tenenbaum, Committee Member.

Advisors/Committee Members: Bahram H. Arjmandi (professor directing thesis), Maria Spicer (committee member), Gershon Tenenbaum (committee member).

Subjects/Keywords: Food

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APA (6^th Edition):

Saadat, R. L. (2010). The Efficacy of Dried Plum in Modulting Biomarkers of Bone Turnover in Postmenopausal Women. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-2143 ;

Chicago Manual of Style (16^th Edition):

Saadat, Raz L. “The Efficacy of Dried Plum in Modulting Biomarkers of Bone Turnover in Postmenopausal Women.” 2010. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-2143 ;.

MLA Handbook (7^th Edition):

Saadat, Raz L. “The Efficacy of Dried Plum in Modulting Biomarkers of Bone Turnover in Postmenopausal Women.” 2010. Web. 28 Feb 2020.

Vancouver:

Saadat RL. The Efficacy of Dried Plum in Modulting Biomarkers of Bone Turnover in Postmenopausal Women. [Internet] [Masters thesis]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2143 ;.

Council of Science Editors:

Saadat RL. The Efficacy of Dried Plum in Modulting Biomarkers of Bone Turnover in Postmenopausal Women. [Masters Thesis]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-2143 ;

Florida State University

10. Hernandez-Escareno, Adali. Comparing the Hormone, Metabolic, and Lipid Profiles of Reproductive Age Women and the Effects of Dried Apple Supplementation and Resistance Exercise in Women with Polycystic Ovarian Syndrome.

Degree: MS, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-4086 ;

► Purpose: The purposes of this study were (1) to compare the androgen and lipid profiles, metabolic syndrome prevalence as well as insulin and HOMA-IR levels… (more)

▼ Purpose: The purposes of this study were (1) to compare the androgen and lipid profiles, metabolic syndrome prevalence as well as insulin and HOMA-IR levels in women with and without PCOS as risk factors for developing cardiovascular disease (CVD) and (2) to study the effects of apple supplementation and resistance exercise on androgen levels, lipid levels, body weight, and metabolic syndrome (MetX) risk factors in women with PCOS. Methods: Baseline data evaluated 22 women with PCOS and 22 height-weight matched controls. Of the 22 women with PCOS, 16 completed the intervention and because of the high dropout rate controls were not included in the intervention. In a 2x2 factorial design using dried apples and resistance exercise, control supplement was 70g of Kellogg's Frosted Mini-Wheats because of similar macronutrient content. Exercise consisted of full body routines twice a week, using 3 sets of 12 repetitions max (12 RM). SPSS for Windows, Version 16.0 software was used to determine descriptive statistics, t-tests, Pearson and Spearman correlations, and one-way ANOVA and factorial ANOVA. Results: At baseline, there was no significant difference between women with PCOS and the controls in age, weight, BMI, Insulin, HOMA-IR, and total T. However, the women with PCOS had significantly higher free T levels and lower SHBG concentrations when compared to the controls (p<0.05). The control women had a more atherogenic lipid profile with significantly higher glucose, total cholesterol, and LDL-C levels than the women with PCOS (p<0.05). The women with PCOS tended to have higher WC than the controls (p<0.1). TG and blood pressure were not significantly different. MetX prevalence was higher (18%) in the controls than the women with PCOS (4.54%). Pearson correlations revealed significant positive correlations between BMI and Insulin/HOMA-IR as well as WC and Insulin/HOMA-IR in all women of this study. Spearman correlation analysis revealed a tendency for FAI and BMI to rank positively in the women with PCOS only (p<0.1). In all women of this study, FAI levels were negatively correlated with SHBG, concurrent to published literature (p<0.001). Intervention results include the following: There was a significant increase in SHBG levels in the women supplemented with apples only (p<0.01) as well as a significant decrease in FAI (p<0.05). Women supplemented with dried apples and participating in the resistance exercise program resulted in significant decreases in total T only (p<0.05) (n=6). Fasting blood glucose was significantly increased in the cereal groups (n=6) after the intervention period and was associated with the higher glycemic index found in the cereal when compared to the apple treatment groups (p<0.05). Increases of vii total cholesterol and LDL-C were seen in the combination therapy and in the control group (no apples-no exercise) (p<0.05). In accordance with other published literature, exercise groups had a tendency to increase HDL-C after the intervention (p<0.1). Conclusion: The women without PCOS were at an… Advisors/Committee Members: Maria T. Spicer (professor directing thesis), Daniel McGee (university representative), Cathy Levenson (committee member).

Subjects/Keywords: Food

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APA (6^th Edition):

Hernandez-Escareno, A. (2010). Comparing the Hormone, Metabolic, and Lipid Profiles of Reproductive Age Women and the Effects of Dried Apple Supplementation and Resistance Exercise in Women with Polycystic Ovarian Syndrome. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-4086 ;

Chicago Manual of Style (16^th Edition):

Hernandez-Escareno, Adali. “Comparing the Hormone, Metabolic, and Lipid Profiles of Reproductive Age Women and the Effects of Dried Apple Supplementation and Resistance Exercise in Women with Polycystic Ovarian Syndrome.” 2010. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-4086 ;.

MLA Handbook (7^th Edition):

Hernandez-Escareno, Adali. “Comparing the Hormone, Metabolic, and Lipid Profiles of Reproductive Age Women and the Effects of Dried Apple Supplementation and Resistance Exercise in Women with Polycystic Ovarian Syndrome.” 2010. Web. 28 Feb 2020.

Vancouver:

Hernandez-Escareno A. Comparing the Hormone, Metabolic, and Lipid Profiles of Reproductive Age Women and the Effects of Dried Apple Supplementation and Resistance Exercise in Women with Polycystic Ovarian Syndrome. [Internet] [Masters thesis]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-4086 ;.

Council of Science Editors:

Hernandez-Escareno A. Comparing the Hormone, Metabolic, and Lipid Profiles of Reproductive Age Women and the Effects of Dried Apple Supplementation and Resistance Exercise in Women with Polycystic Ovarian Syndrome. [Masters Thesis]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-4086 ;

Florida State University

11. Wieder, Ralph Andrew. The Effects of Creatine Supplementation on Muscle Performance during the Transition from an Omnivorous Diet to a Lacto-Ovo Vegetarian Diet.

Degree: MS, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1063 ;

► Converting from an omnivorous (OMNI) diet to a lacto-ovo-vegetarian (LOV) diet has been associated with a decrease in muscle creatine content as a result of… (more)

▼ Converting from an omnivorous (OMNI) diet to a lacto-ovo-vegetarian (LOV) diet has been associated with a decrease in muscle creatine content as a result of the elimination of exogenous creatine intake. Muscle creatine and phosphocreatine are vital in sustaining intense and rapid muscle contractions during exercise. It is unknown if the transition to LOV diet leads to negative impacts on muscle performance due to the reduction of muscle creatine content. PURPOSE: To investigate the effects of creatine supplementation during a 21-day LOV intervention on muscle performance in young males. METHODS: Twenty-eight males (22.2 ± 2.5 yrs old) switched from an OMNI to a LOV diet for 21 days. Participants were assigned into one of two groups: Placebo (Pl; n=15) or Creatine (Cr, 2g; n=13) for 21 days. Muscle strength was evaluated for the upper body by performing 1-repetition maximum (1-RM) tests for the chest press (Smith Machine) and for the lower body by performing maximal voluntary contraction (MVC) of the quadriceps (QUAD) (BiodexTM) before initiation of LOV diet and 21 days after initiation of LOV diet. Muscle endurance for the QUAD was evaluated with two consecutive bouts (bouts 1 and 2) of a fatigue index (FI) test with one minute rest (BiodexTM) at the same time points. Dual-energy-X-ray absorptiometry (DXA) was used to measure body composition at the same time points. Blood samples were collected at same time points. Dietary records were obtained for both OMNI and LOVdiets. RESULTS: There was a group x time interaction (p< 0.05) in FI bout 2 for peak torque, indicating that the QUAD strength for Cr group was changed from 111.62 ± 19.53 (pre) to 121.69 ± 21.5 Newton meters (Nm, post) while that of the Pl group was changed from 138.2 ± 33.08 (pre) to 130.73 ± 32.05 Nm (post) after 21-day LOV intervention. Interestingly, there was a group x time interaction (p=0.05) in FI for percent torque decline during bout 2, indicating that the Cr group changed from -60.75 ± 2.13 (pre) to -61.99 ± 2.53% (post) while Pl group changed from -64.3 ± 2.83 (pre) to -58.64 ± 3.68% (post) after 21-day LOV intervention. There was a group x time interaction (p<0.05) in isometric knee extensions for peak force, indicating that the QUAD strength for Cr group was changed from 204.08 ± 47.05 (pre), to 187.0 ± 43.57 (mid), to 182.0 ± 50.37 Newtons (N,post) while that of the Pl group was changed from 204.87 ± 50.74 (pre), to 211.47 ± 45.36 (mid), to 211.0 ± 53.37 N (post). There was no significant change in body weight, lean body mass, fat mass, total cholesterol, HDL cholesterol, MVC isokinetic extensions at 60, 180, and 300 degrees/second in QUAD, or 1-RM chest press. There was a time effect (p<0.05) indicating a significant decline in protein intake for both groups. CONCLUSIONS: Our findings suggest that 21 days of LOV diet in young males who were previously omnivorous did not have any negative impacts on muscle performance. Daily creatine supplementation (2g/d) during 21-day LOV diet intervention appears to maintain muscle strength during the… Advisors/Committee Members: Jeong-Su Kim (professor directing thesis), Emily M. Haymes (committee member), Robert C. Eklund (committee member), Maria T. Spicer (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wieder, R. A. (2010). The Effects of Creatine Supplementation on Muscle Performance during the Transition from an Omnivorous Diet to a Lacto-Ovo Vegetarian Diet. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-1063 ;

Chicago Manual of Style (16^th Edition):

Wieder, Ralph Andrew. “The Effects of Creatine Supplementation on Muscle Performance during the Transition from an Omnivorous Diet to a Lacto-Ovo Vegetarian Diet.” 2010. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-1063 ;.

MLA Handbook (7^th Edition):

Wieder, Ralph Andrew. “The Effects of Creatine Supplementation on Muscle Performance during the Transition from an Omnivorous Diet to a Lacto-Ovo Vegetarian Diet.” 2010. Web. 28 Feb 2020.

Vancouver:

Wieder RA. The Effects of Creatine Supplementation on Muscle Performance during the Transition from an Omnivorous Diet to a Lacto-Ovo Vegetarian Diet. [Internet] [Masters thesis]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1063 ;.

Council of Science Editors:

Wieder RA. The Effects of Creatine Supplementation on Muscle Performance during the Transition from an Omnivorous Diet to a Lacto-Ovo Vegetarian Diet. [Masters Thesis]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1063 ;

Florida State University

12. Sharma, Girdhari M. Identification and Characterization of Select Allergens in Pecan [Carya Illinoinensis (Wangenh.) K. Koch] and Brazil Nut (Bertholletia Excelsa L.) Seeds.

Degree: PhD, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1788 ;

► Tree nut allergies affect up to 0.2% young children and 0.5% adults in the US. The current investigation focused on two tree nuts (pecan and… (more)

▼ Tree nut allergies affect up to 0.2% young children and 0.5% adults in the US. The current investigation focused on two tree nuts (pecan and Brazil nut), with four specific aims: (i) to clone and characterize 2S albumin, a major allergen in pecan, (ii) to clone and characterize 11S legumin, a major allergen in pecan, (iii) to develop a sensitive and robust competitive ELISA for Brazil nut detection, and (iv) to purify and characterize BN seed allergens. Pecan cDNA expression library was constructed to meet specific aims (i) and (ii). The genes corresponding to 2S albumin and 11S legumin in pecan was amplified and expressed as fusion proteins. The fusion proteins were screened for IgE-binding with pecan allergic human sera. The corresponding native protein in pecan was identified using proteomic tools and inhibition immunoblots. The cross-reactivity of fusion protein with corresponding walnut allergen was also assessed. Overlapping synthetic peptides were used to determine the linear epitopes. Homology modeling of pecan allergens was done to obtain the structural insight and compare with known epitopes of corresponding allergens in other tree nuts. Of the 28 patients' serum IgE tested by dotblot, 22 (79%) bound to 2S albumin (Car i 1) and 16 (57%) bound to 11S legumin (Car i 4). The native pecan 2S albumin is ~16 kDa composed of a large subunit (~12 kDa) linked to small subunit (~4 kDa) by disulfide bond. The native pecan 11S legumin is a hexameric protein, each monomer composed of ~33 kDa acidic subunit linked via disulfide bond to ~20-22 kDa basic subunit. IgE inhibition immunoblots suggested Car i 1 and Car i 4 to be cross-reactive with corresponding walnut allergens Jug r 1 and Jug r 4, respectively. Linear epitope mapping of Car i 1 indicated weak, moderate, and strong reactivity of serum pools against 12, 7, and 5 peptides, respectively. Of the 11 peptides recognized by all 3 serum pools, 5 peptides were strongly reactive. These strongly reactive polypeptides were located in 3 discrete regions of the Car i 1 sequence (residues 43-57, 67-78, and 106-120). Epitope mapping results of Car i 4 revealed weak, moderate, and strong reactivity of serum pools against 10, 5, and 4 peptides, respectively. Seven peptides were recognized by all 3 sera pools, of which 2 were strongly reactive. The strongly reactive peptides were located in 3 discrete regions of the Car i 4 acidic subunit sequence (residues 118-132, 208-219, and 238-249). Homology modeling of Car i 1 and Car i 4 revealed significant overlapping regions shared in common with other tree nuts. Rabbit anti-Brazil nut polyclonal antibodies were used to develop the inhibition ELISA for specific aim (iii). The assay was evaluated for cross-reactivity and robustness. The developed ELISA was sensitive (IC50 = 23.2 ± 9 ng/ml, n=76). Among the 66 tested foods/ingredients, only cinnamon exhibited detectable interference (1.36%). The ELISA could detect Brazil nut seed proteins over a pH range of 5-12, with optimal pH range of 7-10. Exposing Brazil nut seeds to processing… Advisors/Committee Members: Shridhar K. Sathe (professor directing dissertation), Kenneth H. Roux (university representative), Thomas C. S. Keller (committee member), Bahram H. Arjmandi (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sharma, G. M. (2010). Identification and Characterization of Select Allergens in Pecan [Carya Illinoinensis (Wangenh.) K. Koch] and Brazil Nut (Bertholletia Excelsa L.) Seeds. (Doctoral Dissertation). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-1788 ;

Chicago Manual of Style (16^th Edition):

Sharma, Girdhari M. “Identification and Characterization of Select Allergens in Pecan [Carya Illinoinensis (Wangenh.) K. Koch] and Brazil Nut (Bertholletia Excelsa L.) Seeds.” 2010. Doctoral Dissertation, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-1788 ;.

MLA Handbook (7^th Edition):

Sharma, Girdhari M. “Identification and Characterization of Select Allergens in Pecan [Carya Illinoinensis (Wangenh.) K. Koch] and Brazil Nut (Bertholletia Excelsa L.) Seeds.” 2010. Web. 28 Feb 2020.

Vancouver:

Sharma GM. Identification and Characterization of Select Allergens in Pecan [Carya Illinoinensis (Wangenh.) K. Koch] and Brazil Nut (Bertholletia Excelsa L.) Seeds. [Internet] [Doctoral dissertation]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1788 ;.

Council of Science Editors:

Sharma GM. Identification and Characterization of Select Allergens in Pecan [Carya Illinoinensis (Wangenh.) K. Koch] and Brazil Nut (Bertholletia Excelsa L.) Seeds. [Doctoral Dissertation]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-1788 ;

Florida State University

13. Li, Tengfei. Mouse Moloclonal Antibody Based Indirect Elisa for Soybean Lectin (Glycine max L.) Detection.

Degree: MS, Nutrition, Food, and Exercise Science, 2018, Florida State University

URL: http://purl.flvc.org/fsu/fd/2018_Su_Li_fsu_0071N_14667 ;

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Soybeans (Glycine max. L), as one of the most widely grown crops, account for 90% of U.S. oilseed production. Despite their economic, nutritional, and consumer… (more)

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Soybeans (Glycine max. L), as one of the most widely grown crops, account for 90% of U.S. oilseed production. Despite their economic, nutritional, and consumer value, soybeans are among the top eight types of foods responsible for eliciting allergies in sensitive individuals. Soybean lectin is a tetrameric 7S protein with the binding specificity for GalNAc and Gal residues. It is one of the soybean allergens as well as an anti-nutrient that can cause reduced nutritional value of soybean and soybean derived food products. It is also a known toxicant able to agglutinate human red blood cells. Detection of soybean lectin in human and animal food supply is therefore important. The current available methodology for detection and quantification of soybean lectin rely on a variety of techniques that often lack specificity and sensitivity. The objective of this study was to obtain a validated method that is precise and accurate in the measurement of soybean lectin. A mouse monoclonal antibody based indirect ELISA was constructed for soybean lectin detection. Using low pressure column chromatography, soybean (certified seeds Hutcheson) lectin was purified. Using the purified soybean seed lectin, murine mAb 5A5 was produced and purified using Protein-G affinity column chromatography. Borate saline buffer, 0.1 M, pH 8.45 was used to solubilize proteins from petroleum ether defatted seed flour. Dot-blot and Western-blot immunoassays were used to screen mAb 5A5 for soybean lectin detection, specificity and sensitivity. Under the test conditions, the mAb 5A5 was specific for soybean lectin detection. The mAb 5A5, at concentration of 1520 ng/ml and 61 ng/ml, was able to detect 5 and 50 ng of the purified lectin, respectively, using dot- and Western- blot immunoassays. The mAb 5A5 detected the lectin in soluble soy proteins of 58 tested soybean seeds and 11 commercial soybean products using 1 and 10 microgram soy protein respectively for dot- and western blot immunoassays. No cross-reactive protein was found in 21 selected dry beans with mAb 5A5. Using purified murine anti-soybean lectin mAb 5A5 as detection Ab, a mouse monoclonal antibody based indirect ELISA demonstrated sensitivity, specificity, and reliability at trace levels. The indirect ELISA was validated to have LOD of 30 ng/ml (0.03 ppm), protein concentration at 50% máximum signal of 786.3ng/ml. This ELISA assay is reproducible and accurate with CVs <10% in intra- and inter-assay, and the average recoveries within 15% of the actual value. In conclusion, the indirect ELISA is sensitive, specific, and robust for the detection soybean lectin under the test conditions.

A Thesis submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Master of Science.

Summer Semester 2018.

June 21, 2018.

Shridhar K. Sathe, Professor Directing Thesis; Qinchun Rao, Committee Member; Michael Roper, Committee Member.

Advisors/Committee Members: Shridhar K. Sathe (professor directing thesis), Qinchun Rao (committee member), Michael Gabriel Roper (committee member).

Subjects/Keywords: Food

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, T. (2018). Mouse Moloclonal Antibody Based Indirect Elisa for Soybean Lectin (Glycine max L.) Detection. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/2018_Su_Li_fsu_0071N_14667 ;

Chicago Manual of Style (16^th Edition):

Li, Tengfei. “Mouse Moloclonal Antibody Based Indirect Elisa for Soybean Lectin (Glycine max L.) Detection.” 2018. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/2018_Su_Li_fsu_0071N_14667 ;.

MLA Handbook (7^th Edition):

Li, Tengfei. “Mouse Moloclonal Antibody Based Indirect Elisa for Soybean Lectin (Glycine max L.) Detection.” 2018. Web. 28 Feb 2020.

Vancouver:

Li T. Mouse Moloclonal Antibody Based Indirect Elisa for Soybean Lectin (Glycine max L.) Detection. [Internet] [Masters thesis]. Florida State University; 2018. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/2018_Su_Li_fsu_0071N_14667 ;.

Council of Science Editors:

Li T. Mouse Moloclonal Antibody Based Indirect Elisa for Soybean Lectin (Glycine max L.) Detection. [Masters Thesis]. Florida State University; 2018. Available from: http://purl.flvc.org/fsu/fd/2018_Su_Li_fsu_0071N_14667 ;

Florida State University

14. Wang, Danni. The Use of Pangasius Fish in Restaurants.

Degree: MS, Nutrition, Food, and Exercise Science, 2015, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-9520 ;

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Pangasius fish, mainly Pangasius hypophthalmus (tra/swai) and Pangasius bocourti (basa), which belong to the Pangasiidae family of catfish, are imported farm-raised freshwater fish from Asian.… (more)

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Pangasius fish, mainly Pangasius hypophthalmus (tra/swai) and Pangasius bocourti (basa), which belong to the Pangasiidae family of catfish, are imported farm-raised freshwater fish from Asian. Nowadays, the U.S. is one of the largest importers of Pangasius fish worldwide. As mounting quantities of Pangasius fish being imported every year, the names of "tra/swai" and "basa", however, seldom appear on restaurant menus. Since processed fish products in restaurants are not subjected to the requirement of labeling species/origins, it is unclear how and to what extend Pangasius are used in restaurants. The overall objective of this study was to investigate if Pangasius fish has been used as a substitute for domestic catfish (Ictaluridae family), high valued fish species, e.g. grouper and snapper, and fish products without specifying the species on the menu from local restaurants. In total, 47 different fish products from 37 local restaurants in a medium size city were sampled and a commercialized rapid lateral flow strip assay (EZ PangasiusTM kit) were used to identify Pangasius fish. The results showed that 26.7% of domestic catfish tested was substituted by Pangasius, and 22.2% of high-valued fish, grouper, was verified to be Pangasius while no cases of substitution for snapper, sea bass and sole samples tested were found. In addition, a high percentage (66.7%) of dishes displayed under the general name of "fish" on the menu were identified as Pangasius, revealing the favorable choice of imported Pangasius for various fish products due to its sensory quality and cheap price. Isoelectric focusing (IEF) was further conducted to characterize protein patterns of each sample in comparison with authentic fish standards, and the results showed that all the Pangasius positive samples were exclusively tra/swai rather than basa. One year later 7 tested Pangasius positive dishes, including 4 in catfish group and 3 without specifying the species, were sampled again as follow-ups. Indirect non-competitive enzyme linked immunosorbent assay (iELISA), which applied a Pangasius-specific monoclonal antibody (mAb) T7E10, was used to analyze these follow-up samples. The results revealed the continuous use of Pangasius as substitutes for domestic catfish (4 out of 4) and as "anonymous" ingredients for the products without labeling the species. This study exhibited the prevalence of Pangasius in restaurants and also revealed the existence of dishonest behaviors in restaurant business, which called for the need to set up specific measurements and regulations to discourage the fraudulent practice using tra/swai or basa.

A Thesis submitted to the Department of Nutrition, Food and Exercise Science in partial fulfillment of the requirements for the degree of Master of Science.

Summer Semester 2015.

June 2, 2015.

Basa, Fish fraud, Immunoassay, Isoelectric focusing (IEF), Pangasius, Tra/swai

Yun-Hwa Peggy Hsieh, Professor Directing Thesis; Shridhar Sathe, Committee Member; Michael Shatruk, Committee Member.

Advisors/Committee Members: Peggy Hsieh (professor directing thesis), Shridhar K. Sathe (committee member), Mykhailo Shatruk (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, D. (2015). The Use of Pangasius Fish in Restaurants. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-9520 ;

Chicago Manual of Style (16^th Edition):

Wang, Danni. “The Use of Pangasius Fish in Restaurants.” 2015. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-9520 ;.

MLA Handbook (7^th Edition):

Wang, Danni. “The Use of Pangasius Fish in Restaurants.” 2015. Web. 28 Feb 2020.

Vancouver:

Wang D. The Use of Pangasius Fish in Restaurants. [Internet] [Masters thesis]. Florida State University; 2015. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-9520 ;.

Council of Science Editors:

Wang D. The Use of Pangasius Fish in Restaurants. [Masters Thesis]. Florida State University; 2015. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-9520 ;

Florida State University

15. Alderees, Fahad. Assessing the Shelf Life of Retail Shrimp Using Real-Time Microrespirometer.

Degree: MS, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-0160 ;

► Shrimp is the most consumed seafood item in the United States (U.S.). Currently 90% of the shrimp consumed in the U.S. is imported from a… (more)

▼ Shrimp is the most consumed seafood item in the United States (U.S.). Currently 90% of the shrimp consumed in the U.S. is imported from a few Asian countries. When imported shrimp arrives to its destination, it probably contains a load of microbial contamination due to the post-harvest processing steps such as transportation, handling, preparation, beheading, peeling, deveining, packaging and storage that could add further bacterial contamination. Most of the U.S. import refusals belong to seafood shipments due to the detection of bacterial contamination and filthy appearance. Upon shipment arrival, testing for microbial activities of seafood requires a two day incubation period when using the traditional Aerobic Plate Count (APC) method; however, a novel non-instrumental microrespirometer which was developed by Hsieh and Hsieh (2000) can determine the microbial activity of the sample in real-time by measuring the CO2 evolution rate (CER). CO2 is a byproduct of microbial respiration which can be used as a direct indicator of biological activity. The unique characteristic of this method is that it is a simple device that can determine the microbial activity in food less than one hour and is highly sensitive in determining the CER and simple to operate. The use of the microrespirometer instead of the APC in testing the imported seafood shipments will save a great deal of time and lower the cost for both importers and exporters by lowering the testing cost and reducing the costly waiting time at the ports. The specific objectives of this study are: 1) to validate the real-time microrespirometer method by correlating the rapid CER results with the traditional cultural APC method, 2) to establish a shrimp spoilage cut-off value of CER using the microrespirometer method by comparing the results with sensory analysis, 3) to exam the effect of chloramphenicol on shrimp shelf life using non-instrumental microrespirometer, APC method and sensory analysis and 4) to compare the shelf life of farm-raised imported shrimp with domestic wild-caught shrimp using non-instrumental microrespirometer, APC, pH and sensory analysis. Frozen domestic wild-caught shrimp (Penaeus duorarum) and imported farm-raised shrimp (Panaeus vannamei) were purchased locally. Domestic shrimp were treated with chloramohenicol at 10 and 30 ppm and stored at 4°C along with the untreated domestic and imported shrimp. Samples were tested daily using the microrespirometer, APC, pH and olfactory sensory analysis. The p values and correlations between CER, APC and sensory analysis were determined using SPSS Statistic software and Microsoft Excel 2007. The microrespirometer and pH determinations were done in triplicate; the APC was performed in duplicate and the experiments were repeated twice. The CER method was found to be highly correlated with the APC (R²=0.812 to 0.929) for all samples stored at 4°C. When samples' spoilage odor became noticeable, the average CER value of all samples was 27.23 µl/h/g. In order to allow for a small safe margin, a CER value of 25… Advisors/Committee Members: Yun-Hwa Peggy Hsieh (professor directing thesis), Bahram Arjmandi (committee member), Fred W. Huffer (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alderees, F. (2010). Assessing the Shelf Life of Retail Shrimp Using Real-Time Microrespirometer. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-0160 ;

Chicago Manual of Style (16^th Edition):

Alderees, Fahad. “Assessing the Shelf Life of Retail Shrimp Using Real-Time Microrespirometer.” 2010. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-0160 ;.

MLA Handbook (7^th Edition):

Alderees, Fahad. “Assessing the Shelf Life of Retail Shrimp Using Real-Time Microrespirometer.” 2010. Web. 28 Feb 2020.

Vancouver:

Alderees F. Assessing the Shelf Life of Retail Shrimp Using Real-Time Microrespirometer. [Internet] [Masters thesis]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-0160 ;.

Council of Science Editors:

Alderees F. Assessing the Shelf Life of Retail Shrimp Using Real-Time Microrespirometer. [Masters Thesis]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-0160 ;

Florida State University

16. Liu, Changqi. Evaluation of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays (ELISAs) for Tree Nuts and Peanut Detection.

Degree: PhD, Nutrition, Food, and Exercise Science, 2016, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_2016SU_Liu_fsu_0071E_13376 ;

►

Tree nut and peanut allergies affect up to 1.4% US population. Upon exposure, sensitive individuals may experience adverse reactions, including fatal anaphylaxis, to tree nut… (more)

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Tree nut and peanut allergies affect up to 1.4% US population. Upon exposure, sensitive individuals may experience adverse reactions, including fatal anaphylaxis, to tree nut and peanut allergens. Currently, there is no cure for food allergies. Strict avoidance of the offending allergens is the best defense for sensitive individuals. Consequently, it is important to develop reliable assays for food allergen detection. Several commercial polyclonal antibody (pAb)-based immunoassays are available for tree nut and peanut detection. However, the pAb-based assays often exhibit lack of specificity and robustness. The objective of the current investigation was to evaluate sensitivity, specificity, and robustness of monoclonal antibody (mAb)-based sandwich enzyme-linked immunosorbent assays (ELISAs) for almond, cashew, hazelnut, pecan, and peanut detection. The tested ELISAs are sensitive (limit of detections and limit of quantifications < 10 ppm) and reproducible (intra- and inter-assay variabilities < 24%). The laboratory developed ELISA used a mAb (4C10) targeting a well-defined amandin epitope that is recognized by several almond allergic patients’ sera IgE. Compared to the MonoTrace assay, the mAb 4C10-based ELISA was comparable in assay sensitivity and was superior in assay specificity and recognition of a human allergy relevant epitope. The detection mAbs targeted antigens were stable and detectable in whole tree nut and peanut seeds subjected to autoclaving, blanching, frying, microwaving, and dry roasting. No cross-reactivity was observed in 156 food ingredients, each tested at 100,000 ppm except for the hazelnut kit (cross-reactive with pecan and English walnut) and the pecan kit (cross-reactive with English walnut and black walnut). Antigen recovery ranges for spiked and incurred food matrices were 81‒126% and 22‒161%, respectively. The assay results were in agreement with the allergen declaration of 180 tested commercial foods with the exception of one sample where undeclared hazelnuts were detected, two samples where undeclared pecans were detected, and one sample where declared pistachio was not detected. In conclusion, the mAb-based ELISAs are sensitive, specific, and robust for tree nut and peanut detection and quantification under the tested conditions.

A Dissertation submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

Summer Semester 2016.

June 28, 2016.

ELISA, food allergy, monoclonal antibody, peanut, tree nut

Shridhar K. Sathe, Professor Directing Dissertation; John G. Dorsey, University Representative; Yun-Hwa P. Hsieh, Committee Member; Kenneth H. Roux, Committee Member.

Advisors/Committee Members: Shridhar K. Sathe (professor directing dissertation), John G. Dorsey (university representative), Peggy Hsieh (committee member), Kenneth H. Roux (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, C. (2016). Evaluation of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays (ELISAs) for Tree Nuts and Peanut Detection. (Doctoral Dissertation). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_2016SU_Liu_fsu_0071E_13376 ;

Chicago Manual of Style (16^th Edition):

Liu, Changqi. “Evaluation of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays (ELISAs) for Tree Nuts and Peanut Detection.” 2016. Doctoral Dissertation, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_2016SU_Liu_fsu_0071E_13376 ;.

MLA Handbook (7^th Edition):

Liu, Changqi. “Evaluation of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays (ELISAs) for Tree Nuts and Peanut Detection.” 2016. Web. 28 Feb 2020.

Vancouver:

Liu C. Evaluation of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays (ELISAs) for Tree Nuts and Peanut Detection. [Internet] [Doctoral dissertation]. Florida State University; 2016. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_2016SU_Liu_fsu_0071E_13376 ;.

Council of Science Editors:

Liu C. Evaluation of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays (ELISAs) for Tree Nuts and Peanut Detection. [Doctoral Dissertation]. Florida State University; 2016. Available from: http://purl.flvc.org/fsu/fd/FSU_2016SU_Liu_fsu_0071E_13376 ;

Florida State University

17. Zhao, Qing. Lectin Analyses of Soybean and Soybean Products.

Degree: MS, Nutrition, Food, and Exercise Science, 2016, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_2016SU_Zhao_fsu_0071N_13365 ;

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Soybean allergies are one of top eight types of food allergies, affecting ~ 0.4% children and 0.3% adults in North America. Soybean agglutinin (SBA) protein,… (more)

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Soybean allergies are one of top eight types of food allergies, affecting ~ 0.4% children and 0.3% adults in North America. Soybean agglutinin (SBA) protein, also known as soybean lectin, is both an allergen as well as an anti-nutrient that reducing the nutritional value of soybean and soybean products. The objective of this study was to obtain a validated methodology that is precise and accurate in the measurement of SBA while allowing minimally equipped laboratories to effectively conduct the analysis. A competitive ELISA was constructed and optimized by using rabbit anti whole soybean polyclonal antibodies (pAb) as primary Ab. The constructed competitive ELISA was validated to have LOD of 0.063 ng/ml of soybean lectin in all tested samples. The ELISA is reproducible and accurate as CVs of all ELISAs tested were less than 24% and the average recoveries were within 15% of the actual value, which demonstrating accuracy of the assay. However, further investigate is needed to evaluating CV of the assay. The validated ELISA method was able to detect and quantify the SBA in 20 soybean varieties and indicated that the natural variability of SBA is subject to the effects of genotype and environment. Moreover, the ELISA can detect the SBA content in thermal processed soybean, germinated soybean sprouts, and commercial soybean products. The results suggested that processing methods can affect soybean lectin immunoreactivity. Compare to the conventional hemagglutinating assay, ELISA is more sensitive and effective. In conclusion, the constructed pAb-based competitive ELISA provides an efficacious detection and quantification for the soybean lectin.

A Thesis submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Master of Science.

Summer Semester 2016.

June 21, 2016.

Shridhar K. Sathe, Professor Directing Thesis; Qinchun Rao, Committee Member; John G. Dorsey, Committee Member.

Advisors/Committee Members: Shridhar K. Sathe (professor directing thesis), Qinchun Rao (committee member), John G. Dorsey (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, Q. (2016). Lectin Analyses of Soybean and Soybean Products. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_2016SU_Zhao_fsu_0071N_13365 ;

Chicago Manual of Style (16^th Edition):

Zhao, Qing. “Lectin Analyses of Soybean and Soybean Products.” 2016. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_2016SU_Zhao_fsu_0071N_13365 ;.

MLA Handbook (7^th Edition):

Zhao, Qing. “Lectin Analyses of Soybean and Soybean Products.” 2016. Web. 28 Feb 2020.

Vancouver:

Zhao Q. Lectin Analyses of Soybean and Soybean Products. [Internet] [Masters thesis]. Florida State University; 2016. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_2016SU_Zhao_fsu_0071N_13365 ;.

Council of Science Editors:

Zhao Q. Lectin Analyses of Soybean and Soybean Products. [Masters Thesis]. Florida State University; 2016. Available from: http://purl.flvc.org/fsu/fd/FSU_2016SU_Zhao_fsu_0071N_13365 ;

Florida State University

18. Zaffran, Valerie D. Effects of Thermal Processing on Structure and Immunoreactivity of Amandin, Almond (Prunus dulcis L.) Major Protein.

Degree: MS, Nutrition, Food, and Exercise Science, 2016, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_2016SU_Zaffran_fsu_0071N_13369 ;

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Almonds are the number one tree nut produced in the U.S. and the number one consumed tree nut worldwide. Despite their economic, nutritional, and consumer… (more)

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Almonds are the number one tree nut produced in the U.S. and the number one consumed tree nut worldwide. Despite their economic, nutritional, and consumer value, almonds are among the top foods responsible for eliciting allergies in sensitive individuals. Amandin is the major storage protein and allergen in almond, accounting for roughly 70% of the total soluble protein content. Almonds are frequently subjected to heat processing prior to consumption which may potentially alter the structure and allergenic properties of amandin. The objective of this study was to determine how heat treatment influences the structure and immunoreactivity of amandin in thermally processed almond seeds. Whole Nonpareil almond seeds were subjected to autoclaving (121°C, 15 psi, for 15 and 30 min), blanching (94°C for 5 and 10 min), frying (191°C for 1 min), microwaving (1000 W for 1 min and 500 W for 3 min), and roasting (140 and 160°C for 30 min each; and 168 and 177°C for 12 min each). Processed seeds were ground and amandin was isolated from defatted almond flour using cryoprecipitation at 4°C. Soluble protein content was determined by the Bradford and Lowry methods. Immunoreactivity was assessed using enzyme-linked immunosorbent assay (ELISA), Western blot, and dot blot using two different murine monoclonal anti-amandin detection antibodies, 4C10 and 4F10. Structural changes were assessed using ultraviolet (UV) absorption and fluorescence spectroscopy. Results are reported as mean ± SEM and one-way ANOVA was performed to compare means for difference. Post hoc analysis was performed using Fisher’s least significant difference at p ≤ 0.05. ELISA immunoreactivity was assessed by the ratio (R) = signal at 50% maximum signal of processed sample/signal at 50% maximum signal of unprocessed control. The range of R values for the tested processed samples were 0.36 ± 0.02 to 1.54 ± 0.10* for 4C10 (LSD=0.26) and 0.37 ± 0.05* to 1.23 ± 0.17* for 4F10 (LSD=0.21). Western blots and dot blots were consistent with ELISA results. Second-derivative UV spectroscopy was used to quantify tertiary stuctural changes through the determination of derivative peak ratios between two successive peaks. The ratios ranged from 2.31 ± 0.44 to 5.67 ± 0.54. Relative fluorescence intensities (F/F0) ranged from 1.02 to 1.22. The immunoreactivity of amandin in processed almond seeds as assessed by ELISA, Western blot, and dot blot varied depending on the type of processing treatment undergone. While immunoreactivity decreased under certain conditions, it was never eliminated, indicating antigenic stability of amandin towards thermal processing. Under certain processing conditions, changes in second-derivative UV spectra and fluorescence intensity were observed suggesting the occurrence of heat-induced structural changes.

A Thesis submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Master of Science.

Summer Semester 2016.

June 16, 2016.

Almond, Amandin, Immunoreactivity, Protein…

Advisors/Committee Members: Shridhar K. Sathe (professor directing thesis), John G. Dorsey (committee member), Qinchun Rao (committee member), Michael Gabriel Roper (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zaffran, V. D. (2016). Effects of Thermal Processing on Structure and Immunoreactivity of Amandin, Almond (Prunus dulcis L.) Major Protein. (Masters Thesis). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_2016SU_Zaffran_fsu_0071N_13369 ;

Chicago Manual of Style (16^th Edition):

Zaffran, Valerie D. “Effects of Thermal Processing on Structure and Immunoreactivity of Amandin, Almond (Prunus dulcis L.) Major Protein.” 2016. Masters Thesis, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_2016SU_Zaffran_fsu_0071N_13369 ;.

MLA Handbook (7^th Edition):

Zaffran, Valerie D. “Effects of Thermal Processing on Structure and Immunoreactivity of Amandin, Almond (Prunus dulcis L.) Major Protein.” 2016. Web. 28 Feb 2020.

Vancouver:

Zaffran VD. Effects of Thermal Processing on Structure and Immunoreactivity of Amandin, Almond (Prunus dulcis L.) Major Protein. [Internet] [Masters thesis]. Florida State University; 2016. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_2016SU_Zaffran_fsu_0071N_13369 ;.

Council of Science Editors:

Zaffran VD. Effects of Thermal Processing on Structure and Immunoreactivity of Amandin, Almond (Prunus dulcis L.) Major Protein. [Masters Thesis]. Florida State University; 2016. Available from: http://purl.flvc.org/fsu/fd/FSU_2016SU_Zaffran_fsu_0071N_13369 ;

Florida State University

19. Hooshmand, Shirin. Bone Reversal Effects of Plant Bioactive Compounds in Postmenopausal Women.

Degree: PhD, Nutrition, Food, and Exercise Science, 2010, Florida State University

URL: http://purl.flvc.org/fsu/fd/FSU_migr_etd-3788 ;

►

Osteoporosis is a debilitating disorder that affects both female and male, albeit to a greater extent in women than men. As the demographic shift to… (more)

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Osteoporosis is a debilitating disorder that affects both female and male, albeit to a greater extent in women than men. As the demographic shift to a more aged population continues, a growing number of men and women will be afflicted with osteoporosis. Therefore, search for potential non-pharmacological alternative therapies for osteoporosis is of prime interest. Aside from existing drug therapies, certain lifestyle and nutritional factors are known to reduce the risk of osteoporosis. Among nutritional factors, recent observations suggest that dried plum, or prunes (Prunus domestica L.) is the most effective fruit in both preventing and reversing bone loss. Several animal studies, a 3-month and a one-year long clinical trials conducted in our laboratories have shown that dried plum has positive effects on bone indices and bone mineral density (BMD). The animal data indicate that dried plum not only prevents but more importantly reverses bone loss in two separate models of osteopenia. Our initial animal study indicated that dried plum prevented the ovariectomy-induced BMD reduction of the femur and lumbar vertebra. In another study rats were ovariectomized and allowed to lose bone before the initiation of treatment to mimic established osteoporosis. Dried plum as low as 5% (w/w) gram per kilogram diet restored BMD to the level of intact rats. Dried plum also reversed the loss of trabecular architectural properties such as trabecular number, connectivity density, and trabecular separation. We have also shown the effectiveness of dried plum in reversal of bone loss due to skeletal unloading. Microcomputed tomography (µCT) analyses revealed that dried plum enhances bone recovery during reambulation following skeletal unloading and has comparable effects to parathyroid hormone. In addition to the animal studies, our 3-month clinical trial indicated that the consumption of dried plum daily significantly modulated serum markers of bone turnover in postmenopausal women.

A Dissertation submitted to the Department of Nutrition, Food and Exercise Sciences in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

Spring Semester, 2010.

March 3, 2010.

Nutrition, Functional Food, Phytochemicals

Bahram H. Arjmandi, Professor Directing Dissertation; Kenneth Brummel-Smith, University Representative; Peggy Y. Hsieh, Committee Member.

Advisors/Committee Members: Bahram H. Arjmandi (professor directing dissertation), Kenneth Brummel-Smith (university representative), Peggy Y. Hsieh (committee member).

Subjects/Keywords: Food

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hooshmand, S. (2010). Bone Reversal Effects of Plant Bioactive Compounds in Postmenopausal Women. (Doctoral Dissertation). Florida State University. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-3788 ;

Chicago Manual of Style (16^th Edition):

Hooshmand, Shirin. “Bone Reversal Effects of Plant Bioactive Compounds in Postmenopausal Women.” 2010. Doctoral Dissertation, Florida State University. Accessed February 28, 2020. http://purl.flvc.org/fsu/fd/FSU_migr_etd-3788 ;.

MLA Handbook (7^th Edition):

Hooshmand, Shirin. “Bone Reversal Effects of Plant Bioactive Compounds in Postmenopausal Women.” 2010. Web. 28 Feb 2020.

Vancouver:

Hooshmand S. Bone Reversal Effects of Plant Bioactive Compounds in Postmenopausal Women. [Internet] [Doctoral dissertation]. Florida State University; 2010. [cited 2020 Feb 28]. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-3788 ;.

Council of Science Editors:

Hooshmand S. Bone Reversal Effects of Plant Bioactive Compounds in Postmenopausal Women. [Doctoral Dissertation]. Florida State University; 2010. Available from: http://purl.flvc.org/fsu/fd/FSU_migr_etd-3788 ;

University of Otago

20. Smith, Claire. Barriers and Solutions to Achieving Food Security for New Zealand Families .

Degree: 2011, University of Otago

URL: http://hdl.handle.net/10523/2010

► Food insecurity is defined as: “limited or uncertain availability of nutritionally adequate and safe foods or limited ability to acquire acceptable foods in a socially… (more)

Subjects/Keywords: food security; food insecurity; family food environment; food purchasing; food access

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APA (6^th Edition):

Smith, C. (2011). Barriers and Solutions to Achieving Food Security for New Zealand Families . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/2010

Chicago Manual of Style (16^th Edition):

Smith, Claire. “Barriers and Solutions to Achieving Food Security for New Zealand Families .” 2011. Doctoral Dissertation, University of Otago. Accessed February 28, 2020. http://hdl.handle.net/10523/2010.

MLA Handbook (7^th Edition):

Smith, Claire. “Barriers and Solutions to Achieving Food Security for New Zealand Families .” 2011. Web. 28 Feb 2020.

Vancouver:

Smith C. Barriers and Solutions to Achieving Food Security for New Zealand Families . [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/10523/2010.

Council of Science Editors:

Smith C. Barriers and Solutions to Achieving Food Security for New Zealand Families . [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/2010

North-West University

21. Van Wyk, Dorette. Exploring household food security in the Vaalharts area / Dorette van Wyk .

Degree: 2014, North-West University

URL: http://hdl.handle.net/10394/15725

► Food security is essential amongst households as it enables consumers to live a healthy and productive life. Determining factors for households to be classified as… (more)

Subjects/Keywords: Food security; Food knowledge; Food consumption; Food handling; Food safety

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APA (6^th Edition):

Van Wyk, D. (2014). Exploring household food security in the Vaalharts area / Dorette van Wyk . (Thesis). North-West University. Retrieved from http://hdl.handle.net/10394/15725

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van Wyk, Dorette. “Exploring household food security in the Vaalharts area / Dorette van Wyk .” 2014. Thesis, North-West University. Accessed February 28, 2020. http://hdl.handle.net/10394/15725.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van Wyk, Dorette. “Exploring household food security in the Vaalharts area / Dorette van Wyk .” 2014. Web. 28 Feb 2020.

Vancouver:

Van Wyk D. Exploring household food security in the Vaalharts area / Dorette van Wyk . [Internet] [Thesis]. North-West University; 2014. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/10394/15725.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van Wyk D. Exploring household food security in the Vaalharts area / Dorette van Wyk . [Thesis]. North-West University; 2014. Available from: http://hdl.handle.net/10394/15725

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

22. [No author]. Key factors driving the foraging ecology of Oribi : fear, cattle and the quality and quantity of food.

Degree: 2015, University of KwaZulu-Natal

URL: http://hdl.handle.net/10413/12239

► Body size and digestive physiology are two factors that influence herbivores foraging behaviour, habitat use and potential to compete with other animals. The aim of… (more)

▼ Body size and digestive physiology are two factors that influence herbivores foraging behaviour, habitat use and potential to compete with other animals. The aim of this study was to determine how seasonal changes in grass quality, interactions with cattle, and perceived predation risk influenced oribi (Ourebia ourebi) foraging and landscape use. Oribi are one of the smallest pure grazing ruminants. As a result, they should select high quality vegetation. In line with this, I found that throughout the study oribi fed selectively over multiple spatial scales ranging from plant parts to habitats. By focussing on green grass within these different scales, oribi were able to maintain their crude protein intake needed for maintenance. Throughout South Africa, oribi frequently interact with cattle. Due to their differences in body size and nutritional requirements, there should be sufficient resource partitioning to avoid competition. However, I found that the nature of the interspecific interactions (i.e. competition or facilitation) between these species depended on season and cattle stocking rates. During the wet season, cattle facilitated oribi by providing high quality regrowth. However, at high stocking rates, cattle indirectly competed with oribi during the dry season via the impacts of their wet season grazing. Specifically, intense wet season grazing by cattle reduced the availability of high quality grass for oribi in the dry season. This was not the case at low and intermediate stocking rates. Differences in food availability and predation risk across habitats can influence how herbivores utilise landscapes. I found that predation risk greatly affected oribi foraging behaviour, with oribi preferring to feed in safer rather than riskier habitats. However, when food availability increased in these risky habitats, oribi increased their risk-taking behaviour at both small- and large-scales within these risky areas. Ultimately, this suggests that oribi trade-off between predation risk and food availability. Finally, to link my results to the management and conservation of this vulnerable antelope, I applied the knowledge I gained from the above research to a case study. Advisors/Committee Members: Shrader, Adrian Morgan (advisor).

Subjects/Keywords: Herbivores – Food.; Antelopes – Food.; Animals – Food.

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APA (6^th Edition):

author], [. (2015). Key factors driving the foraging ecology of Oribi : fear, cattle and the quality and quantity of food. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/12239

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Key factors driving the foraging ecology of Oribi : fear, cattle and the quality and quantity of food. ” 2015. Thesis, University of KwaZulu-Natal. Accessed February 28, 2020. http://hdl.handle.net/10413/12239.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Key factors driving the foraging ecology of Oribi : fear, cattle and the quality and quantity of food. ” 2015. Web. 28 Feb 2020.

Vancouver:

author] [. Key factors driving the foraging ecology of Oribi : fear, cattle and the quality and quantity of food. [Internet] [Thesis]. University of KwaZulu-Natal; 2015. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/10413/12239.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Key factors driving the foraging ecology of Oribi : fear, cattle and the quality and quantity of food. [Thesis]. University of KwaZulu-Natal; 2015. Available from: http://hdl.handle.net/10413/12239

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

23. Tuttle, Charlotte. Household decisions related to food access and expenditure: essays on food insecurity and SNAP participation in the United States.

Degree: PhD, Applied Economics, 2013, University of Minnesota

URL: http://purl.umn.edu/159307

► Recent trends in food insecurity and participation in the Supplemental Nutrition Assistance Program have highlighted the need to re-examine the effectiveness of the current SNAP… (more)

Subjects/Keywords: Food expenditure; Food security; Food stamps

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APA (6^th Edition):

Tuttle, C. (2013). Household decisions related to food access and expenditure: essays on food insecurity and SNAP participation in the United States. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/159307

Chicago Manual of Style (16^th Edition):

Tuttle, Charlotte. “Household decisions related to food access and expenditure: essays on food insecurity and SNAP participation in the United States.” 2013. Doctoral Dissertation, University of Minnesota. Accessed February 28, 2020. http://purl.umn.edu/159307.

MLA Handbook (7^th Edition):

Tuttle, Charlotte. “Household decisions related to food access and expenditure: essays on food insecurity and SNAP participation in the United States.” 2013. Web. 28 Feb 2020.

Vancouver:

Tuttle C. Household decisions related to food access and expenditure: essays on food insecurity and SNAP participation in the United States. [Internet] [Doctoral dissertation]. University of Minnesota; 2013. [cited 2020 Feb 28]. Available from: http://purl.umn.edu/159307.

Council of Science Editors:

Tuttle C. Household decisions related to food access and expenditure: essays on food insecurity and SNAP participation in the United States. [Doctoral Dissertation]. University of Minnesota; 2013. Available from: http://purl.umn.edu/159307

Penn State University

24. Williams, Kezi. FATE OF LISTERIA MONOCYTOGENES ON INTACT AND WOUNDED APPLES DURING STORAGE AND IN A SIMULATED DUMP TANK.

Degree: 2018, Penn State University

URL: https://etda.libraries.psu.edu/catalog/15357kvw5404

► The effect of fruit carnauba wax and shellac fruit coatings on the fate of Listeriamonocytogenes inoculated onto whole fresh apples during storage was studied. Littleinformation… (more)

▼ The effect of fruit carnauba wax and shellac fruit coatings on the fate of Listeriamonocytogenes inoculated onto whole fresh apples during storage was studied. Littleinformation exists on the effectiveness of commercial fruit coatings for reducing L.monocytogenes populations on fruit. There is also little information on the effect of poststorageparameters, such as temperature, relative humidity, apple variety, and wounddamage on fate of L. monocytogenes on stored apples nor the degree to which crosscontamination is limited when apples are submerged in sanitizer solutions, such asoccurs in apple dump tanks and flumes. To address these issues, studies were done todetermine: 1) if commercially-available carnauba wax or shellac coatings affect L.monocytogenes growth; 2) the fate of L. monocytogenes populations on intact andwounded fresh apples during refrigerated or ambient temperature; and 3) the level of L.monocytogenes destruction and cross-contamination that occurs in a simulated dumptank.In the first study, a plate overlay assay method was used to determine ifcarnauba wax or shellac formulations had any effect on growth and survival of L.monocytogenes. Solid pellets were prepared by thoroughly drying the coatings in plasticmolds to eliminate any potential antimicrobial effects of the carrier solvents present inthe formulations. Results from this study showed no zones of inhibition or signs ofgrowth enhancement after 24 and 48 hours incubation, thus indicating that neithercoating had negative or positive effects on L. monocytogenes growth.ivTo follow up on these preliminary results, the fate of L. monocytogenes duringrefrigerated (4 °C) storage of coated or un-coated apples for up to 28 days wasdetermined. In addition to coating effects, variables tested were incorporation of 0.1%lauric arginate into the coating formulations, direct application of 0.1% aqueous lauricarginate onto apples prior to storage, and skin wounding,. Wounds were created tosimulate damage to apples that could occur during transportation or movement alongthe packing line. Significant (p<0.05) decreases in L. monocytogenes were observed forall treatments and the control during the 28-day storage period at 4 °C and 50% RH.There were no significant differences between the two coating types and the control, norwere there differences between the two coating types, thus confirming the results of theplate assay. Incorporation of 0.1% lauric arginate in either coating formulation onlyslightly increased the rate of L. monocytogenes destruction on intact inoculation sites,but not on the wounded sites. Overall, the aqueous lauric arginate resulted in a greaterreduction rates compared to the same antimicrobial emulsified into the fruit coatings.In another experiment of this study, the effects of storage temperature (4 or 20 °C), storage relative humidity (50 or 89 % RH), and apple variety (Fuji or McIntosh) onintact or wounded inoculation sties were studied. No significant differences in L.monocytogenes populations were observed after 28 days for either… Advisors/Committee Members: Dr. Luke LaBorde, Thesis Advisor.

Subjects/Keywords: Food Science; Food Microbiology; Food Safety

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APA (6^th Edition):

Williams, K. (2018). FATE OF LISTERIA MONOCYTOGENES ON INTACT AND WOUNDED APPLES DURING STORAGE AND IN A SIMULATED DUMP TANK. (Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/15357kvw5404

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Williams, Kezi. “FATE OF LISTERIA MONOCYTOGENES ON INTACT AND WOUNDED APPLES DURING STORAGE AND IN A SIMULATED DUMP TANK.” 2018. Thesis, Penn State University. Accessed February 28, 2020. https://etda.libraries.psu.edu/catalog/15357kvw5404.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Williams, Kezi. “FATE OF LISTERIA MONOCYTOGENES ON INTACT AND WOUNDED APPLES DURING STORAGE AND IN A SIMULATED DUMP TANK.” 2018. Web. 28 Feb 2020.

Vancouver:

Williams K. FATE OF LISTERIA MONOCYTOGENES ON INTACT AND WOUNDED APPLES DURING STORAGE AND IN A SIMULATED DUMP TANK. [Internet] [Thesis]. Penn State University; 2018. [cited 2020 Feb 28]. Available from: https://etda.libraries.psu.edu/catalog/15357kvw5404.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Williams K. FATE OF LISTERIA MONOCYTOGENES ON INTACT AND WOUNDED APPLES DURING STORAGE AND IN A SIMULATED DUMP TANK. [Thesis]. Penn State University; 2018. Available from: https://etda.libraries.psu.edu/catalog/15357kvw5404

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Helsinki

25. Wu, Rui. Heterologous human intrinsic factor expression from probiotic yeast Saccharomyces boulardii.

Degree: Department of Food and Environmental Sciences; Helsingfors universitet, Agrikultur- och forstvetenskapliga fakulteten, Institutionen för livsmedels- och miljövetenskaper, 2017, University of Helsinki

URL: http://hdl.handle.net/10138/231906

► Saccharomyces cerevisiae var. boulardii is a probiotic strain of the baker’s yeast Saccharomyces cerevisiae. It has a long history of use in treating and preventing… (more)

▼ Saccharomyces cerevisiae var. boulardii is a probiotic strain of the baker’s yeast Saccharomyces cerevisiae. It has a long history of use in treating and preventing several kinds of diarrhea in human. Recently, scientists have drawn their attentions to use this probiotic yeast as a living drug delivery vehicle to the gastric intestinal tract (GIT). Several distinctive features of S. boulardii such as an optimal growth temperature at 37 ºC and better acidic tolerance enable active recombinant protein expression in harsh conditions. Human intrinsic factor (IF) is a glycoprotein secreted by gastric parietal cells and belongs to vitamin B12 (Cobalamin, Cbl) transport family. The gastric IF gene (GIF) encodes this protein. It is an essential protein for the proper absorption of Cbl in the terminal ileum, lacking of which results in vitamin B12 deficiency, especially common among the elders. This study aims to express active human IF from probiotic S. boulardii that can be combined with vitamin B12 forming B12/IF complex and absorbed by intestinal cells. To achieve these goals, a plasmid pSF-TEF1-TPI1-Blast-GIF was designed for S. boulardii transformation. Verification of recombinant IF was accomplished by SDS-PAGE, Western blotting and peptide sequence mapping by mass spectrometry (MS). Experimental results indicated that the recombinant IF was not able to be synthesized in useful amount from S. boulardii using plasmid pSF-TEF1-TPI1-Blast-GIF. Transformation of the plasmid carrying GIF sequence brought significant phenotypic and metabolic modification to the host cells. PCR technique was found unsuccessful to verify the presence of correct plasmid, leaving uncertainty whether the correct plasmid was present in the transformants or not. Interestingly, an immunreoactive band at expected size (45 kDa) could be detected with rat polyclonal antibody in Western blotting. This band could be only detected from the supernatant when cells were grown with Cbl supplement, but shown to be exo-1,3-β-glucanase in MS protein sequencing. However, it is still possible that trace amount of recombinant IFs are secreted but cannot be detected due to MS sequencing limitations. Even though there was a contradictory result in Western blotting compared to that of MS sequencing, the excessive secretion of the exo-1,3-β-glucanase which may corrode yeast cell walls, may explain the different phenotype of transformants in comparison to host cells. This study indicated that not all genetic manipulation tools designed for S. cerevisiae are without problems for S. boulardii. More studies need to be carried out for successful heterologous IF protein expression with this probiotic yeast.

Subjects/Keywords: Food Science; Food Science; Food Science

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APA (6^th Edition):

Wu, R. (2017). Heterologous human intrinsic factor expression from probiotic yeast Saccharomyces boulardii. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/231906

Chicago Manual of Style (16^th Edition):

Wu, Rui. “Heterologous human intrinsic factor expression from probiotic yeast Saccharomyces boulardii.” 2017. Masters Thesis, University of Helsinki. Accessed February 28, 2020. http://hdl.handle.net/10138/231906.

MLA Handbook (7^th Edition):

Wu, Rui. “Heterologous human intrinsic factor expression from probiotic yeast Saccharomyces boulardii.” 2017. Web. 28 Feb 2020.

Vancouver:

Wu R. Heterologous human intrinsic factor expression from probiotic yeast Saccharomyces boulardii. [Internet] [Masters thesis]. University of Helsinki; 2017. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/10138/231906.

Council of Science Editors:

Wu R. Heterologous human intrinsic factor expression from probiotic yeast Saccharomyces boulardii. [Masters Thesis]. University of Helsinki; 2017. Available from: http://hdl.handle.net/10138/231906

University of Southern California

26. Boyle, Alecxandra. Nostalgia: more than just the flavor of the week: a critical look at the movement of nostalgic food from "trend" to "mainstream".

Degree: MA, Strategic Public Relations, 2014, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/363150/rec/4456

► The culinary evolution of nostalgic food was studied and supported with palatable evidence from past and present popular restaurants and food and beverage brands, which… (more)

Subjects/Keywords: nostalgia; nostalgic food; food trends; comfort food

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APA (6^th Edition):

Boyle, A. (2014). Nostalgia: more than just the flavor of the week: a critical look at the movement of nostalgic food from "trend" to "mainstream". (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/363150/rec/4456

Chicago Manual of Style (16^th Edition):

Boyle, Alecxandra. “Nostalgia: more than just the flavor of the week: a critical look at the movement of nostalgic food from "trend" to "mainstream".” 2014. Masters Thesis, University of Southern California. Accessed February 28, 2020. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/363150/rec/4456.

MLA Handbook (7^th Edition):

Boyle, Alecxandra. “Nostalgia: more than just the flavor of the week: a critical look at the movement of nostalgic food from "trend" to "mainstream".” 2014. Web. 28 Feb 2020.

Vancouver:

Boyle A. Nostalgia: more than just the flavor of the week: a critical look at the movement of nostalgic food from "trend" to "mainstream". [Internet] [Masters thesis]. University of Southern California; 2014. [cited 2020 Feb 28]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/363150/rec/4456.

Council of Science Editors:

Boyle A. Nostalgia: more than just the flavor of the week: a critical look at the movement of nostalgic food from "trend" to "mainstream". [Masters Thesis]. University of Southern California; 2014. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/363150/rec/4456

University of Helsinki

27. Han, Xiaocui. Chitin analysis of insects (mealworm and cricket).

Degree: Department of Food and Environmental Sciences; Helsingfors universitet, Agrikultur- och forstvetenskapliga fakulteten, Institutionen för livsmedels- och miljövetenskaper, 2018, University of Helsinki

URL: http://hdl.handle.net/10138/233314

► The literature review dealt with the chemical and physical properties of chitin polymer and its degradation product glucosamine. Particular emphasis was given to studies on… (more)

Subjects/Keywords: Food Science; Food Science; Food Science

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APA (6^th Edition):

Han, X. (2018). Chitin analysis of insects (mealworm and cricket). (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/233314

Chicago Manual of Style (16^th Edition):

Han, Xiaocui. “Chitin analysis of insects (mealworm and cricket).” 2018. Masters Thesis, University of Helsinki. Accessed February 28, 2020. http://hdl.handle.net/10138/233314.

MLA Handbook (7^th Edition):

Han, Xiaocui. “Chitin analysis of insects (mealworm and cricket).” 2018. Web. 28 Feb 2020.

Vancouver:

Han X. Chitin analysis of insects (mealworm and cricket). [Internet] [Masters thesis]. University of Helsinki; 2018. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/10138/233314.

Council of Science Editors:

Han X. Chitin analysis of insects (mealworm and cricket). [Masters Thesis]. University of Helsinki; 2018. Available from: http://hdl.handle.net/10138/233314

Massey University

28. Zhang, Enchong. The development of hotcake products with reduced staling and reduction of microbiological growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand.

Degree: 2012, Massey University

URL: http://hdl.handle.net/10179/4039

► Staling and microbiological spoilage are major issues in the market development of hotcake products. This project is aiming at reduce the staling rate of hotcake… (more)

Subjects/Keywords: Pancakes; Food spoilage; Food preservatives; Food staling

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APA (6^th Edition):

Zhang, E. (2012). The development of hotcake products with reduced staling and reduction of microbiological growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/4039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Enchong. “The development of hotcake products with reduced staling and reduction of microbiological growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. ” 2012. Thesis, Massey University. Accessed February 28, 2020. http://hdl.handle.net/10179/4039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Enchong. “The development of hotcake products with reduced staling and reduction of microbiological growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. ” 2012. Web. 28 Feb 2020.

Vancouver:

Zhang E. The development of hotcake products with reduced staling and reduction of microbiological growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. [Internet] [Thesis]. Massey University; 2012. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/10179/4039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang E. The development of hotcake products with reduced staling and reduction of microbiological growth : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. [Thesis]. Massey University; 2012. Available from: http://hdl.handle.net/10179/4039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

29. -5199-8515. A food system plan for Austin.

Degree: MSin Community and Regional Planning, Environmental Planning, 2015, University of Texas – Austin

URL: http://hdl.handle.net/2152/41493

► Austin, TX is a city with goals for a strong community food system, as expressed in the Imagine Austin comprehensive plan. The City proposes creating… (more)

Subjects/Keywords: Food; Food system planning; Community food system

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APA (6^th Edition):

-5199-8515. (2015). A food system plan for Austin. (Masters Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/41493

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

-5199-8515. “A food system plan for Austin.” 2015. Masters Thesis, University of Texas – Austin. Accessed February 28, 2020. http://hdl.handle.net/2152/41493.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

-5199-8515. “A food system plan for Austin.” 2015. Web. 28 Feb 2020.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-5199-8515. A food system plan for Austin. [Internet] [Masters thesis]. University of Texas – Austin; 2015. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/2152/41493.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

-5199-8515. A food system plan for Austin. [Masters Thesis]. University of Texas – Austin; 2015. Available from: http://hdl.handle.net/2152/41493

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

University of Gothenburg / Göteborgs Universitet

30. Post, Anna. Nordic stakeholders and sustainable catering.

Degree: 2011, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/24100

► Sustainability deficits in modern food systems are widely reported and changes in food habits towards more sustainable eating patterns, including eating seasonal and locally produced… (more)

Subjects/Keywords: food system; sustainable food; organic food; purchasers

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APA (6^th Edition):

Post, A. (2011). Nordic stakeholders and sustainable catering. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/24100

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Post, Anna. “Nordic stakeholders and sustainable catering.” 2011. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed February 28, 2020. http://hdl.handle.net/2077/24100.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Post, Anna. “Nordic stakeholders and sustainable catering.” 2011. Web. 28 Feb 2020.

Vancouver:

Post A. Nordic stakeholders and sustainable catering. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2011. [cited 2020 Feb 28]. Available from: http://hdl.handle.net/2077/24100.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Post A. Nordic stakeholders and sustainable catering. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2011. Available from: http://hdl.handle.net/2077/24100

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations