subject:(Follicular fluid)

Mississippi State University

1. Muth-Spurlock, Ashleigh Marie. Follicular waves and their impact on the dominant follicle, uterus, and subsequent pregnancy rates in beef cattle.

Degree: PhD, Animal and Dairy Sciences, 2016, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06212016-103045/ ;

► Ovarian steroids assist in the development of the follicle and its enclosed oocyte as well as prepare the maternal environment for pregnancy. The objective… (more)

▼ Ovarian steroids assist in the development of the follicle and its enclosed oocyte as well as prepare the maternal environment for pregnancy. The objective of experiment 1 was to elucidate the differences between dominant follicles of each follicular wave in terms of intra-follicular concentration of steroids after 4 d of dominance. Differences in blood perfusion between the dominant follicle of the first and second non-ovulatory wave were also examined. Follicular waves were monitored daily via ultrasonography from emergence to aspiration of the dominant follicle. It was determined that although the dominant follicles aspirated from ovulatory waves possessed a greater concentration of estradiol and a greater ratio of estradiol to progesterone, there was no difference in concentrations of steroid hormones or the ratio of estradiol to progesterone between dominant follicles collected from non-ovulatory waves and ovulatory waves. In a subset of cows, blood perfusion tended to be greater in dominant follicles that developed during the second non-ovulatory wave. The objective of experiment 2 was to determine whether or not the follicular wave had an effect on diameter of the ovulatory follicle, thickness of the endometrium, or subsequent pregnancy rates. Estrus was synchronized in females in such a way that females would ovulate the dominant follicle of the first or second follicular wave at timed artificial insemination. Diameter of the ovulatory follicle and endometrial thickness were not different between treatments; however, increased pregnancy rates were observed in heifers that ovulated the dominant follicle of the second follicular wave. There was no effect of follicular wave on pregnancy rates in cows. In conclusion, the role of follicular wave on the dominant follicle, maternal environment, and subsequent pregnancy rates is not fully understood. Additional experiments need to be conducted to further elucidate the differences in developmental potential of the oocyte and maternal environment when the dominant follicle of the first and second wave are destined to become the ovulatory follicle at timed insemination. Advisors/Committee Members: Jeremy Block (committee member), Jean M. N. Feugang (committee member), Jamie Larson (chair), Caleb O. Lemley (committee member).

Subjects/Keywords: beef cattle; steroid hormones; follicular fluid; dominant follicle; follicular waves

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APA (6^th Edition):

Muth-Spurlock, A. M. (2016). Follicular waves and their impact on the dominant follicle, uterus, and subsequent pregnancy rates in beef cattle. (Doctoral Dissertation). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-06212016-103045/ ;

Chicago Manual of Style (16^th Edition):

Muth-Spurlock, Ashleigh Marie. “Follicular waves and their impact on the dominant follicle, uterus, and subsequent pregnancy rates in beef cattle.” 2016. Doctoral Dissertation, Mississippi State University. Accessed March 06, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-06212016-103045/ ;.

MLA Handbook (7^th Edition):

Muth-Spurlock, Ashleigh Marie. “Follicular waves and their impact on the dominant follicle, uterus, and subsequent pregnancy rates in beef cattle.” 2016. Web. 06 Mar 2021.

Vancouver:

Muth-Spurlock AM. Follicular waves and their impact on the dominant follicle, uterus, and subsequent pregnancy rates in beef cattle. [Internet] [Doctoral dissertation]. Mississippi State University; 2016. [cited 2021 Mar 06]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06212016-103045/ ;.

Council of Science Editors:

Muth-Spurlock AM. Follicular waves and their impact on the dominant follicle, uterus, and subsequent pregnancy rates in beef cattle. [Doctoral Dissertation]. Mississippi State University; 2016. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-06212016-103045/ ;

2. Moura, Diego Souza [UNESP]. Análise proteômica do líquido folicular de vacas gestantes.

Degree: 2016, Universidade Estadual Paulista

URL: http://hdl.handle.net/11449/146670

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No ciclo estral das vacas ocorre uma série de eventos que se repetem até o impedimento da luteólise, pela presença do embrião no útero, que… (more)

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No ciclo estral das vacas ocorre uma série de eventos que se repetem até o impedimento da luteólise, pela presença do embrião no útero, que provoca alterações no organismo materno de ordens hormonais, anatômicas e comportamentais. Apesar disso, a colheita de oócitos em vacas gestantes é uma alternativa para a produção in vitro de embriões. No entanto, em vista do perfil hormonal durante a gestação, os fatores envolvidos no desenvolvimento folicular podem ser comprometidos. Em vista disso, este estudo objetivou descrever o perfil proteico do líquido folicular de vacas gestantes. Foram colhidos ovários de 36 vacas gestantes em terço inicial de gestação. O líquido folicular foi puncionado e o diâmetro de cada folículo foi mensurado pela ultrassonografia, sendo classificados em três categorias ≤6,4mm, 6,5mm a 8,9mm e ≥9. Após duas centrifugações para remoção dos componentes celulares, as proteínas totais foram mensuradas e a eletroforese unidimensional realizada sob condições desnaturantes e redutoras. As bandas foram recortadas e a digestão in gel realizada objetivando a espectrometria de massas. A concentração de estrógeno e progesterona foi mensurada no líquido folicular para determinar a viabilidade dos folículos. Foram encontradas 46 bandas de proteínas na eletroforese em 67 amostras de líquido folicular. A média da concentração ± desvio padrão da progesterona foi de 71,32 ± 80,06 ng/mL e estrógeno de 27,57 ± 30,62 ng/mL, considerando todos os folículos. Houve expressão diferenciada de proteínas nas diferentes categorias de folículos. Na espectrometria de massas foram encontradas diversas proteínas, sendo as principais apolipoproteina, angiotensinogênio, heat shock protein, glutationa e suas isoformas.

Estorus cycle of cows has a series of events that are repeated until the luteolysis impediment, because the presence of embryo in the uterus, which causes hormonal, anatomical and behavioral orders, changes in the maternal organism. Nevertheless, the ovum pick-up in pregnant cows is an alternative to the in vitro production of embryos. However, in view of the hormonal profile during pregnancy, the factors involved in follicular development may be impaired. Thus, this study aimed to describe the protein profile of follicular fluid from pregnant cows. Ovaries were collected from 36 pregnant cows with gestational age 1-4 months. Follicular fluid was punctured and the diameter of each follicle was measured by ultrasonography, classified into three categories ≤6.4mm, 6.5 to 8.9 mm and ≥9. After two centrifugation to remove the cellular components, total protein were measurement and unidimensional electrophoresis was performed under denaturing and reducing conditions. The bands were cut and digestion in gel was carried out aiming to mass spectrometry. The concentration of estrogen and progesterone in follicular fluid was measured to determine the viability of follicles. We found 46 protein bands in electrophoresis in 67 follicular fluid samples. The mean ± standard deviation of…

Advisors/Committee Members: Souza, Fabiana Ferreira de [UNESP], Universidade Estadual Paulista (UNESP).

Subjects/Keywords: OPU; Folículo; Proteínas; Fluído folicular; Bovinos; Prenhez; Follicular; Protein; Follicular fluid; Bovine

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APA (6^th Edition):

Moura, D. S. [. (2016). Análise proteômica do líquido folicular de vacas gestantes. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/146670

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moura, Diego Souza [UNESP]. “Análise proteômica do líquido folicular de vacas gestantes.” 2016. Thesis, Universidade Estadual Paulista. Accessed March 06, 2021. http://hdl.handle.net/11449/146670.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moura, Diego Souza [UNESP]. “Análise proteômica do líquido folicular de vacas gestantes.” 2016. Web. 06 Mar 2021.

Vancouver:

Moura DS[. Análise proteômica do líquido folicular de vacas gestantes. [Internet] [Thesis]. Universidade Estadual Paulista; 2016. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/11449/146670.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moura DS[. Análise proteômica do líquido folicular de vacas gestantes. [Thesis]. Universidade Estadual Paulista; 2016. Available from: http://hdl.handle.net/11449/146670

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

3. Pantasri, Tawiwan. Ovarian follicular fluid reflects the clinical condition and oocyte cumulus homeostasis.

Degree: 2012, University of Adelaide

URL: http://hdl.handle.net/2440/85985

► Infertility is a worldwide problem that is often overlooked. Although assisted reproductive technology has been developed over decades, many women still suffer from infertility. More… (more)

▼ Infertility is a worldwide problem that is often overlooked. Although assisted reproductive technology has been developed over decades, many women still suffer from infertility. More knowledge is needed to understand ovarian homeostasis to optimise fertility treatment. This study aimed to explore the relationship of lipids and glucose levels in blood and follicular fluid, and compare these substrates among women with normal or abnormal metabolic condition. It also sought to measure lipid content within human oocytes as well as the expression of endoplasmic reticulum stress marker genes in cumulus cells, and their relationship with metabolic substances, obesity and IVF outcome. The blood, follicular fluid, cumulus cells and unfertilised oocytes from 88 women, who underwent IVF in FertilitySA from February 2011 to August 2011, were collected and analysed for glucose, lipids and endoplasmic reticulum stress markers. Follicular glucose, insulin, high density lipoprotein cholesterol (HDL-C) and majority of polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid (MUFA) levels correlated with the serum levels (r= 0.16-0.23). Insulin was associated with the BMI, waist circumference, metabolic syndrome and many fatty acids, but not follicular glucose. However, the immaturity rate of the retrieved oocytes correlated with the follicular glucose and total fatty acids (r = 0.19-0.26, p <0.04). Variability of the unfertilised oocyte morphology correlated with follicular glucose, and the immaturity rate also differed among the metabolic syndrome group. An increase of follicular 18:3 n-3 (alpha-linolenic) and decrease of 20:3 n-3 (eicosatrienoic acid; ETA) existed in women with a waist circumference of more than 80 cm. The follicular 20:5 n-3 (eicosapentaenoic acid; EPA) percentage correlated with fertilisation and cleavage rate (r = -0.32, p = 0.003 and r = -0.35, p = 0.001). The follicular low density lipoprotein cholesterol (LDL-C) and HDL-C related to follicular fatty acids. Higher levels of serum LDL-C (2.31 ± 0.65 and 1.98 ± 0.61mmol/L, p 0.02) and follicular fatty acid (0.26 ± 0.09 and 0.22 ± 0.05 mmol/L, p = 0.03) were found in non-pregnant women. There was a wide variability of ER stress expression in cumulus cells among women in this study. There was no obvious correlation between ER stress markers and other measurements. The unfertilised oocyte BODIPY fluorescence intensity had high variability among women and individuals. However, the unfertilised oocyte lipid content correlated with the serum LDL-C level. Substances with a good follicular-serum relationship may be transported directly from blood to the follicle. The discorrelation might be affected by intrafollicular metabolism. Insulin may be involved in follicular lipid metabolism because it correlated with many follicular fatty acids and cholesterols. The equilibrium between follicular fatty acids involving insulin modulation may affect oocyte quality. Overall, this study found there were correlations between serum and follicular lipids, follicular insulin… Advisors/Committee Members: Norman, Robert John (advisor), Robker, Rebecca Louise (advisor), School of Paediatrics and Reproductive Health (school).

Subjects/Keywords: obesity; female reproduction; glucose; lipids; follicular fluid; oocyte

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APA (6^th Edition):

Pantasri, T. (2012). Ovarian follicular fluid reflects the clinical condition and oocyte cumulus homeostasis. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/85985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pantasri, Tawiwan. “Ovarian follicular fluid reflects the clinical condition and oocyte cumulus homeostasis.” 2012. Thesis, University of Adelaide. Accessed March 06, 2021. http://hdl.handle.net/2440/85985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pantasri, Tawiwan. “Ovarian follicular fluid reflects the clinical condition and oocyte cumulus homeostasis.” 2012. Web. 06 Mar 2021.

Vancouver:

Pantasri T. Ovarian follicular fluid reflects the clinical condition and oocyte cumulus homeostasis. [Internet] [Thesis]. University of Adelaide; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2440/85985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pantasri T. Ovarian follicular fluid reflects the clinical condition and oocyte cumulus homeostasis. [Thesis]. University of Adelaide; 2012. Available from: http://hdl.handle.net/2440/85985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

4. Medenica, Sanja, 1984- 30677863. Uticaj prisustva tiroid-peroksidaznih antitela na ishod vantelesne oplodnje.

Degree: Medicinski fakultet, 2019, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:20680/bdef:Content/get

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Medicina - Endokrinologija / Medicine - Endocrinology

Iako postoje podaci koji povezuju tiroidnu autoimunost (TAI) i infertilitet, podaci koji se odnose na ishode vantelesne oplodnje… (more)

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Medicina - Endokrinologija / Medicine - Endocrinology

Iako postoje podaci koji povezuju tiroidnu autoimunost (TAI) i infertilitet, podaci koji se odnose na ishode vantelesne oplodnje (VTO) i markere TAI u folikularnoj tečnosti žena koje ulaze u program VTO su retki. Ciljevi studije su bili: ispitati prisustvo tiroidnih autoantitela u folikularnoj tečnosti kod pacijentkinja sa TAI, oceniti uticaj TAI na postizanje fertiliteta metodom VTO i proceniti povezanost nivoa tiroidnih autoantitela u folikularnoj tečnosti i ishoda VTO izraženog kao postignute trudnoće. Metod: Studija je uključila 52 žene koje su ušle u program VTO (26 ispitanica sa TAI i 26 ispitanica kontrolne grupe bez TAI). Krv je uzorkovana pre početka protokola za kontrolisanu ovarijalnu stimulaciju, a u serumu su merene vrednosti tireotropina (TSH), slobodnog trijodtironina (fT3), slobodnog tiroksina (fT4), antitela na tiroidnu peroksidazu (anti TPO At) i antitela na tireoglobulin (anti Tg At). U folikularnoj tečnosti su merene vrednosti TSH, fT4, anti TPO At, anti Tg At i progesterona. Rezultati: U trenutku ulaska u studiju sve pacijentkinje su bile nulipare, osim jedne koja je bila unipara. Sve pacijentkinje su imale primarni infertilitet, osim jedne sa sekundarnim infertilitetom. Prosečno trajanje infertiliteta bilo je duže u grupi pacijentkinja koje nemaju TAI (p=0,050). Naši rezultati su pokazali da su prosečne serumske vrednosti TSH (p=0,010) i fT3 (p=0,001) bile značajno više, a prosečna serumska vrednost fT4 (p=0,008) niža u TAI negativnoj grupi. TAI pozitivna grupa pacijentkinja je imala značajno više vrednosti anti TPO At i anti Tg At u serumu i folikularnoj tečnosti, u poređenju sa TAI negativnom grupom (p<0,001). Nije bilo značajnih razlika između grupa prema prosečnim vrednostima TSH i fT4 u folikularnoj tečnosti. Pokazana je statistički značajna korelacija nivoa serumskih i anti TPO At u folikularnoj tečnosti (0,961, p<0,001 u grupi sa TAI; 0,438, p=0,025 u grupi bez TAI) i serumskih i anti Tg At u folikularnoj tečnosti (0,945, p<0,001 u grupi sa TAI; 0,554, p=0,003 u grupi bez TAI). Stopa trudnoće po započetom ciklusu i embriotransferu značajno se razlikovala između grupe sa i bez TAI, (30,8% vs 61,5%), p=0,026 i (34,8% vs 66,7%), p=0,029, redom...

Advisors/Committee Members: Žarković, Miloš, 1960- 12563815.

Subjects/Keywords: follicular fluid; thyroid autoimmunity; in vitro fertilization; infertility; progesterone

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Medenica, Sanja, 1. 3. (2019). Uticaj prisustva tiroid-peroksidaznih antitela na ishod vantelesne oplodnje. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:20680/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Medenica, Sanja, 1984- 30677863. “Uticaj prisustva tiroid-peroksidaznih antitela na ishod vantelesne oplodnje.” 2019. Thesis, Univerzitet u Beogradu. Accessed March 06, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:20680/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Medenica, Sanja, 1984- 30677863. “Uticaj prisustva tiroid-peroksidaznih antitela na ishod vantelesne oplodnje.” 2019. Web. 06 Mar 2021.

Vancouver:

Medenica, Sanja 13. Uticaj prisustva tiroid-peroksidaznih antitela na ishod vantelesne oplodnje. [Internet] [Thesis]. Univerzitet u Beogradu; 2019. [cited 2021 Mar 06]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:20680/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Medenica, Sanja 13. Uticaj prisustva tiroid-peroksidaznih antitela na ishod vantelesne oplodnje. [Thesis]. Univerzitet u Beogradu; 2019. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:20680/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad de Extremadura

5. Macedo Morales, María. Papel de la melatonina en el estado oxidativo del líquido folicular y su relación con la calidad de los oocitos en mujeres con problemas de fertilidad .

Degree: 2017, Universidad de Extremadura

URL: http://hdl.handle.net/10662/6463

► La melatonina y sus metabolitos protegen del daño oxidativo tanto de forma directa, secuestrando especies reactivas, como indirecta, estimulando el sistema enzimático antioxidante. En particular,… (more)

Subjects/Keywords: Melatonina; Estrés oxidativo; Líquido folicular; Melatonin; Oxidative stress; Follicular fluid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Macedo Morales, M. (2017). Papel de la melatonina en el estado oxidativo del líquido folicular y su relación con la calidad de los oocitos en mujeres con problemas de fertilidad . (Thesis). Universidad de Extremadura. Retrieved from http://hdl.handle.net/10662/6463

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Macedo Morales, María. “Papel de la melatonina en el estado oxidativo del líquido folicular y su relación con la calidad de los oocitos en mujeres con problemas de fertilidad .” 2017. Thesis, Universidad de Extremadura. Accessed March 06, 2021. http://hdl.handle.net/10662/6463.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Macedo Morales, María. “Papel de la melatonina en el estado oxidativo del líquido folicular y su relación con la calidad de los oocitos en mujeres con problemas de fertilidad .” 2017. Web. 06 Mar 2021.

Vancouver:

Macedo Morales M. Papel de la melatonina en el estado oxidativo del líquido folicular y su relación con la calidad de los oocitos en mujeres con problemas de fertilidad . [Internet] [Thesis]. Universidad de Extremadura; 2017. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10662/6463.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Macedo Morales M. Papel de la melatonina en el estado oxidativo del líquido folicular y su relación con la calidad de los oocitos en mujeres con problemas de fertilidad . [Thesis]. Universidad de Extremadura; 2017. Available from: http://hdl.handle.net/10662/6463

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Andrade, Gabriella Mamede. Prospecção retrospectiva de lipídos presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos.

Degree: Mestrado, Qualidade e Produtividade Animal, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-29012015-093100/ ;

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A produção in vitro de embriões, no Brasil, ocupa lugar de destaque dentre as biotecnologias da reprodução animal e o atual desafio é torná-la cada… (more)

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A produção in vitro de embriões, no Brasil, ocupa lugar de destaque dentre as biotecnologias da reprodução animal e o atual desafio é torná-la cada vez mais acessível, para tanto, é fundamental aproximar os resultados de produção e sobrevivência dos embriões produzidos in vitro daqueles desenvolvidos naturalmente. Boa parte desta variação do potencial de desenvolvimento do embrião está relacionada à características do oócito. Há a possibilidade de essa capacidade estar relacionada ao perfil lipídico do líquido folicular e das células do folículo, neste sentido, este projeto teve como objetivo principal avaliar a correlação do potencial de desenvolvimento de oócitos com o conteúdo do perfil lipídico no líquido folicular e das células foliculares, baseado no sistema de ativação partenogenética, cultivo individual e analise retrospectiva. Não existe, até o momento, uma caracterização detalhada do microambiente folicular, um componente importante dos fluídos são os lipídios e recentemente tem sido demonstrado que eles estão envolvidos em vários aspectos do metabolismo além de também funcionarem como moléculas sinalizadoras ou até mesmo serem capazes de induzir modificações na cromatina. Os íons oriundos do estudo do perfil lipídico foram avaliados em comparação ao potencial de desenvolvimento do oócito. No conjunto de dados do MALDI observou-se maior concentração de triacilglicerídeos, principalmente contendo ácidos graxos como o oleico, linoleico e esteárico nos líquidos foliculares e células do folículo provenientes do ambiente folicular de oócitos que clivaram. No perfil lipídico do líquido folicular feito por LC-MS/MS os lipídios identificados foram de classes diversas, com participação expressiva no modo positivo de triacilgliceróis, fosfatidilcolinas, esfingomielinas, monoacildiacilglicerídeos entre outros e no modo negativo a classe lipídica que prevaleceu foi dos lipídios mitocondriais, as cardiolipinas. Também foram detectados lipídios citosólicos como o CE e alguns pouco descritos no líquido folicular como o PIP2 e PIP3, além disso, alguns lipídios foram característicos dos grupos clivados e blastocistos. O perfil lipídico do líquido folicular e das células do folículo é complexo e alguns lipídios deste ambiente tem potencial para serem biomarcadores de qualidade oocitária.

In vitro embryo production in Brazil occupies a prominent place among the animal reproduction biotechnology, the current challenge is to make it increasingly accessible so it is essential to approach the results of production and survival of in vitro produced embryos of those developed naturally. Much of this potential change of embryo development is related to the oocyte characteristics. There is the possibility of this ability is related to the lipid profile of follicular fluid and follicle cells , in this sense, this project aimed to evaluate the correlation of the oocytes development potential with the content of the lipid profile in follicular fluid and cells follicular, based on parthenogenetic activation , individual cultivation…

Advisors/Committee Members: Meirelles, Flávio Vieira.

Subjects/Keywords: Células do folículo; Follicle cells; Follicular fluid; Lipídios; Lipids; Líquido folicular; Oocyte quality; Qualidade oocitária

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrade, G. M. (2014). Prospecção retrospectiva de lipídos presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/74/74131/tde-29012015-093100/ ;

Chicago Manual of Style (16^th Edition):

Andrade, Gabriella Mamede. “Prospecção retrospectiva de lipídos presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos.” 2014. Masters Thesis, University of São Paulo. Accessed March 06, 2021. http://www.teses.usp.br/teses/disponiveis/74/74131/tde-29012015-093100/ ;.

MLA Handbook (7^th Edition):

Andrade, Gabriella Mamede. “Prospecção retrospectiva de lipídos presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos.” 2014. Web. 06 Mar 2021.

Vancouver:

Andrade GM. Prospecção retrospectiva de lipídos presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos. [Internet] [Masters thesis]. University of São Paulo; 2014. [cited 2021 Mar 06]. Available from: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-29012015-093100/ ;.

Council of Science Editors:

Andrade GM. Prospecção retrospectiva de lipídos presentes no líquido folicular associados ao potencial de desenvolvimento dos oócitos. [Masters Thesis]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-29012015-093100/ ;

University of Toronto

7. Lau, Angela. The Impact of Follicular Fluid on Oviductal Epithelial Cell Gene Expression.

Degree: 2014, University of Toronto

URL: http://hdl.handle.net/1807/72561

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Recent evidence indicates high-grade serous ovarian cancer originates from the fallopian tube epithelium (FTE). Risk factors include germline mutations in breast cancer susceptibility genes, BRCA1… (more)

Subjects/Keywords: Ovarian Cancer; Oviduct; Fallopian Tube; BRCA1; Ovulation; Follicular Fluid; Bovine; Primary Cell Culture; 0380

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lau, A. (2014). The Impact of Follicular Fluid on Oviductal Epithelial Cell Gene Expression. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/72561

Chicago Manual of Style (16^th Edition):

Lau, Angela. “The Impact of Follicular Fluid on Oviductal Epithelial Cell Gene Expression.” 2014. Masters Thesis, University of Toronto. Accessed March 06, 2021. http://hdl.handle.net/1807/72561.

MLA Handbook (7^th Edition):

Lau, Angela. “The Impact of Follicular Fluid on Oviductal Epithelial Cell Gene Expression.” 2014. Web. 06 Mar 2021.

Vancouver:

Lau A. The Impact of Follicular Fluid on Oviductal Epithelial Cell Gene Expression. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1807/72561.

Council of Science Editors:

Lau A. The Impact of Follicular Fluid on Oviductal Epithelial Cell Gene Expression. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/72561

Université de Sherbrooke

8. Gervais, Alexandre. Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques: Implication of ovarian lipotoxicity in the polycystic ovary syndrome.

Degree: 2014, Université de Sherbrooke

URL: http://hdl.handle.net/11143/5897

► Résumé : Le syndrome des ovaires polykystiques (SOPK) affecte 6-10% des femmes en âge de procréer et est la principale cause d’infertilité anovulatoire. Cette condition… (more)

▼ Résumé : Le syndrome des ovaires polykystiques (SOPK) affecte 6-10% des femmes en âge de procréer et est la principale cause d’infertilité anovulatoire. Cette condition est principalement caractérisée par une hyperandrogénie provenant des ovaires et des glandes surrénales. De plus en plus d’études, à la fois in vivo et in vitro, indiquent qu’une surexposition ovarienne en gras, de même que les mécanismes lipotoxiques subséquents pourraient causer l’hyperandrogénie chez ces femmes. Ainsi, cette étude vise à 1) évaluer l’implication des gras et de l’inflammation folliculaire dans la production des androgènes ovariens par une étude de corrélation; et 2) comparer les niveaux folliculaires de gras, métabolites lipidiques et marqueurs inflammatoires entre les femmes SOPK et non-SOPK. Cette étude transversale a recruté 80 femmes en cours de fécondation in vitro. Treize étaient SOPK, 58 étaient non-SOPK et 9 avaient un diagnostic incertain. Les participantes au projet avaient un âge moyen de 33 ± 4 ans. Tout groupe confondus, les niveaux folliculaires de testostérone corrélaient avec les gras (r=0,381; P=0,001; indépendamment de l’IL-6), les acylcarnitines (r≥0,255; tout P=0,008; non indépendamment des gras) et l’IL-6 (r=0,300; P=0,009; indépendamment des gras). De plus, les niveaux folliculaires de gras corrélaient avec les acylcarnitines (r≥0,594; tout P<0,001). Lorsque comparées aux femmes non-SOPK, les femmes SOPK étaient plus obèses (BMI: 31,2 vs. 25,1 kg/m2; P=0,009) et avaient des niveaux folliculaires plus élevés de testostérone (12,9 vs. 0,29 nM; P=0,001), de gras (P=0,001), d’acylcarnitines (tout P=0,001) et du marqueur inflammatoire IL-6 (13,0 vs. 8.6 pg/mL; P=0,018), le tout indépendamment de l’IMC. Les résultats suggèrent qu’une exposition intra-ovarienne en gras pourrait contribuer à une augmentation de la production des androgènes. Les mécanismes pourraient inclure une β-oxydation insuffisante, illustré par des acylcarnitines élevés, menant à l’accumulation de métabolites lipidiques toxiques, et une inflammation ovarienne, possiblement suite à une infiltration de macrophages. Ces deux mécanismes semblent être indépendants. De plus, l’environnement ovarien des femmes SOPK est caractérisé par une élévation des gras, de métabolites lipidiques et de marqueurs inflammatoires. Il peut donc être proposer que l’hyperandrogénie caractéristique du SOPK soit causée en partie par la lipotoxicité. // Abstract : Polycystic ovary syndrome (PCOS) affects 6-10% of the women of childbearing age and is the main cause of anovulatory infertility. It is mainly characterised by hyperandrogenism that can originate from the adrenal gland and the ovaries. A growing number of evidences, either in vivo or in vitro, points toward an overexposition to fat and lipotoxic mechanisms (lipid induced cellular toxicity) as the cause of PCOS hyperandrogenesis. Therefore, we aimed to: 1) evaluate the implication of follicular fluid (FF) levels of fat and inflammation markers in the ovarian production of androgens through a correlation analysis;… Advisors/Committee Members: Baillargeon, Jean-Patrice (advisor).

Subjects/Keywords: Lipotoxicité; Androgénèse; Liquide folliculaire; Syndrome des ovaires polykystiques; Lipotoxicity; Androgenesis; Follicular fluid; Polycystic ovary syndrome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gervais, A. (2014). Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques: Implication of ovarian lipotoxicity in the polycystic ovary syndrome. (Masters Thesis). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/5897

Chicago Manual of Style (16^th Edition):

Gervais, Alexandre. “Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques: Implication of ovarian lipotoxicity in the polycystic ovary syndrome.” 2014. Masters Thesis, Université de Sherbrooke. Accessed March 06, 2021. http://hdl.handle.net/11143/5897.

MLA Handbook (7^th Edition):

Gervais, Alexandre. “Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques: Implication of ovarian lipotoxicity in the polycystic ovary syndrome.” 2014. Web. 06 Mar 2021.

Vancouver:

Gervais A. Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques: Implication of ovarian lipotoxicity in the polycystic ovary syndrome. [Internet] [Masters thesis]. Université de Sherbrooke; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/11143/5897.

Council of Science Editors:

Gervais A. Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques: Implication of ovarian lipotoxicity in the polycystic ovary syndrome. [Masters Thesis]. Université de Sherbrooke; 2014. Available from: http://hdl.handle.net/11143/5897

9. Hadjopoulos, Grigorios. Επίδραση της χορήγησης GnRH αγωνιστών στο ωοθηκικό περιβάλλον και τη γονιμότητα γυναικών που πάσχουν από ενδομητρίωση.

Degree: 2020, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/48002

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Objective: To evaluate the effects of GnRH-a on women’s fertility with mild endometriosis, undergoing IVF-ET procedures. Design: Prospective, randomized, controlled trial. Setting: Three tertiary University… (more)

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Objective: To evaluate the effects of GnRH-a on women’s fertility with mild endometriosis, undergoing IVF-ET procedures. Design: Prospective, randomized, controlled trial. Setting: Three tertiary University Hospitals. Patients: Four hundred infertile women with mild endometriosis, documented withlaparoscopy, undergoing an IVF attempt and 200 women with tubal factor infertility. Interventions: Patients with endometriosis were divided randomly in women who received GnRH-a for three months before an IVF attempt (group A; n=200) and who did not receive GnRH-a (Group B; n=200). Main Outcome Measures: TNF-a, IL-1β, IL-6, IL-8 and IL-1-ra were measured in the follicular fluid (FF) of all women. Fertilization Rate (FR), Implantation Rate (IR), quality of embryos, and clinical Pregnancy Rate (PR) were evaluated. Results: Women who received GnRH-a had significantly reduced FF’s cytokines concentration comparing with women who did not receive this regime. Women of group B presented reduced FRs compared to women of group A and to women with tubal infertility . Embryo quality, IR, and clinical PR did not significantly improve in women of group A compared with women of group B. Conclusion: Women who received for three months GnRH-a had lower FF’s cytokinesconcentration. These women had also higher FR than women who did not receive GnRH-a. In contrast, IR, quality of embryos, and clinical PR were not statistically significant different among women of two groups.

Σκοπός: Η αξιολόγηση της επίδρασης των αγωνιστών της εκλυτικής ορμόνης των γοναδοτροπινών (GnRH-a) στη γονιμότητα σε γυναίκες με ήπια ενδομητρίωση που υποβλήθηκαν σε εξωσωματική γονιμοποίηση-εμβρυομεταφορά. Σχεδιασμός :Προοπτική, τυχαιοποιημένη, ελεγχόμενη μελέτη.Υλοποίηση: Σε τρία Τριτοβάθμια Νοσοκομεία. Ασθενείς: Τετρακόσιες υπογόνιμες γυναίκες με ήπια ενδομητρίωση τεκμηριωμένη με λαπαροσκόπηση, οι οποίες υποβλήθηκαν σε διενέργεια εξωσωματικής γονιμοποίησης και διακόσιες γυναίκες με σαλπιγγικό παράγοντα υπογονιμότηταςπου αποτέλεσαν την ομάδα ελέγχου. Μέθοδος :Οι ασθενείς με ήπιας βαρύτητας ενδομητρίωση , χωρίστηκαν τυχαιοποιημένα σε δύο ομάδες: α) ασθενείς στις οποίες χορηγήθηκε θεραπεία με GnRH-a για τρεις μήνες πριν τη προσπάθεια εξωσωματικής γονιμοποίησης (ομάδα Α, n=200) και β) ασθενείς στις οποίες δεν χορηγήθηκε GnRh-a(Ομάδα Β, n=200). Παράμετροι που μετρήθηκαν: Παράγοντας νέκρωσης όγκων -α TNF-a, ιντερλευκίνη IL-1b ,IL-6, IL-8 και ανταγωνιστής υποδοχέων ιντερλευκίνης IL-1-ra, μετρήθηκαν στο ωοθυλακικό υγρό(FF) σε όλες τις γυναίκες. Ο Δείκτης Γονιμοποίησης (FR), ο Δείκτης Εμφύτευσης (IR), η ποιότητα των εμβρύων και τα ποσοστά κλινικά επιβεβαιωμένων κυήσεων (PR) καταγράφηκαν και αξιολογήθηκαν επίσης. Αποτελέσματα: Οι γυναίκες που έλαβαν τη θεραπεία με GnRH-a εμφάνισαν στατιστικώς σημαντική ελάττωση της συγκέντρωσης κυτοκινών στο ωοθυλακικό υγρό (FF) συγκριτικά με τις γυναίκες που δεν έλαβαν αυτή τη θεραπεία . Οι γυναίκες της Ομάδας Β παρουσίασαν μειωμένα ποσοστά γονιμοποίησης (FR) σε σύγκριση με τις γυναίκες της Ομάδας Α και με τις γυναίκες με υπογονιμότητα…

Subjects/Keywords: Ενδομητρίωση; Κυτοκίνες; Υπογονιμότητα; Ωοθυλακικό υγρό; Cytokines; Endometriosis; Follicular fluid; GnRH-a; Infertility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hadjopoulos, G. (2020). Επίδραση της χορήγησης GnRH αγωνιστών στο ωοθηκικό περιβάλλον και τη γονιμότητα γυναικών που πάσχουν από ενδομητρίωση. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/48002

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hadjopoulos, Grigorios. “Επίδραση της χορήγησης GnRH αγωνιστών στο ωοθηκικό περιβάλλον και τη γονιμότητα γυναικών που πάσχουν από ενδομητρίωση.” 2020. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed March 06, 2021. http://hdl.handle.net/10442/hedi/48002.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hadjopoulos, Grigorios. “Επίδραση της χορήγησης GnRH αγωνιστών στο ωοθηκικό περιβάλλον και τη γονιμότητα γυναικών που πάσχουν από ενδομητρίωση.” 2020. Web. 06 Mar 2021.

Vancouver:

Hadjopoulos G. Επίδραση της χορήγησης GnRH αγωνιστών στο ωοθηκικό περιβάλλον και τη γονιμότητα γυναικών που πάσχουν από ενδομητρίωση. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2020. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10442/hedi/48002.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hadjopoulos G. Επίδραση της χορήγησης GnRH αγωνιστών στο ωοθηκικό περιβάλλον και τη γονιμότητα γυναικών που πάσχουν από ενδομητρίωση. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2020. Available from: http://hdl.handle.net/10442/hedi/48002

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

10. Weber, Matheus Nunes. Identificação, caracterização e análise filogenética de pestivírus em bovinos.

Degree: 2013, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/69933

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Atualmente, o Brasil é o segundo maior exportador de carne bovina e o sexto exportador de leite no mundo. Visando melhorar a produtividade da bovinocultura,… (more)

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Atualmente, o Brasil é o segundo maior exportador de carne bovina e o sexto exportador de leite no mundo. Visando melhorar a produtividade da bovinocultura, torna-se importante a aplicação de programas sanitários adequados e eficientes. As doenças virais são preocupações constantes em qualquer programa de sanidade, devido aos grandes prejuízos econômicos que causam e o seu diagnóstico correto depende de técnicas laboratoriais eficientes. O gênero Pestivirus, da família Flaviviridae, possui três espécies reconhecidas que podem infectar bovinos: o vírus da diarreia viral bovina tipo 1 (BVDV-1), o BVDV-2 e o vírus da doença da fronteira (BDV), além de uma espécie atípica proposta como BVDV-3. Causam principalmente distúrbios respiratórios, reprodutivos e digestivos em bovinos, levando a queda de índices de produção. Os objetivos do presente trabalho foram: descrever o uso de uma técnica modificada de detecção de agentes virais que possam estar presentes em insumos utilizados na reprodução assistida e descrever a caracterização patológica e análise filogenética de pestivírus detectados em um surto da doença, sendo os dados apresentados sob a forma de dois artigos. No primeiro, pools de líquidos foliculares colhidos durante a punção ovariana de vacas para coleta de oócitos para produção in vitro de embriões foram selecionados. As amostras foram analisadas para a detecção de BVDV, herpesvirus bovino tipo 1 (BoHV-1) e 5 (BoHV-5) por isolamento viral, RT-PCR e nested PCR, sendo utilizados dois protocolos de extração de DNA e RNA: um convencional e o outro com adição de etapas pré-extração de ácido nucleico, no intuito de reduzir possíveis inibidores de RT-PCR e PCR presentes nas amostras. A utilização da técnica de pré-extração permitiu detectar um maior número de amostras positivas. No segundo trabalho, foi descrito um surto de diarreia viral bovina em um rebanho que foi testado por imunohistoquímica, ELISA, isolamento viral, RT-PCR e sequenciamento de três regiões do genoma. Foram obtidas quatro amostras distintas de BVDV-3 e as principais alterações clinicas foram encontradas nos sistemas digestivo e respiratório, mas lesões de pele e opacidade de córnea também foram observadas. Os resultados encontrados nos trabalhos realizados reforçam a necessidade da verificação quanto à presença de patógenos em materiais biológicos utilizados na reprodução assistida utilizando técnicas sensíveis e que a doença causada por BVDV-3 em bovinos é indistinguível clinicamente das causadas por outros pestivírus, sugerindo que pestivírus atípicos podem estar subdiagnosticados nos rebanhos brasileiros.

Currently, Brazil is the second largest beef and the sixth milk exporter worldwide. To improve the livestock productivity, it becomes important the applying of appropriate and efficient health programs. Viral diseases are constant concerns on any health program due to major economic losses they cause and their correct diagnosis depends of the development of efficient diagnostic techniques. The genus Pestivirus of the family Flaviviridae, has…

Advisors/Committee Members: Canal, Cláudio Wageck.

Subjects/Keywords: Pestivirus : Diagnostico; Follicular fluid; Doencas : Bovinos; Pestivirus; Patologia animal : Bovinos; BVDV; PCR; Virologia veterinaria : Bovinos; Bovine; Diagnosis; Pathology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weber, M. N. (2013). Identificação, caracterização e análise filogenética de pestivírus em bovinos. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/69933

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Weber, Matheus Nunes. “Identificação, caracterização e análise filogenética de pestivírus em bovinos.” 2013. Thesis, Universidade do Rio Grande do Sul. Accessed March 06, 2021. http://hdl.handle.net/10183/69933.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Weber, Matheus Nunes. “Identificação, caracterização e análise filogenética de pestivírus em bovinos.” 2013. Web. 06 Mar 2021.

Vancouver:

Weber MN. Identificação, caracterização e análise filogenética de pestivírus em bovinos. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2013. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10183/69933.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Weber MN. Identificação, caracterização e análise filogenética de pestivírus em bovinos. [Thesis]. Universidade do Rio Grande do Sul; 2013. Available from: http://hdl.handle.net/10183/69933

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

11. Bezerra, Glícia Pinheiro. Associação entre as concentrações de antígenos leucocitários humanos-G em fluído folicular e a qualidade do embrião avaliada pelo critério de escore embrionário graduado em ciclos de fertilização in vitro.

Degree: 2014, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/127400

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Introdução: A taxa da prevalência da infertilidade em casais com idade reprodutiva atinge 8-10%. A fim de tentar contornar esse problema, esses casais optam pela… (more)

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Introdução: A taxa da prevalência da infertilidade em casais com idade reprodutiva atinge 8-10%. A fim de tentar contornar esse problema, esses casais optam pela reprodução assistida. Em muitos dos casos, os resultados das técnicas de reprodução assistida (TRA) são bem sucedidos ao realizarem a fertilização in vitro (FIV), em outros não são satisfatórios ou ocorrem gestações múltiplas que aumenta o risco de morbidade a essas gestantes. Diante deste quadro, estudam-se formas de aperfeiçoar as TRA no que se refere à seleção do embrião. O hormônio anti-Mülleriano (AMH) já desempenha este papel ao refletir a reserva ovariana na rotina das clínicas de FIV. Os antígenos leucocitários humanos-G solúveis (sHLA-G) vêm sendo investigado no seu papel em refletir a qualidade embrionária. A existência da associação entre as concentrações de HLA-G mensuradas em fluido folicular e a qualidade embrionária avaliada pelo critério de pontuação Graduated Embryo Score (GES) poderia caracterizar o sHLA-G como um possível biomarcador de qualidade embrionária. Objetivos: Investigar a associação entre as concentrações do sHLA-G no fluido folicular (FF) e a pontuação dos embriões gerados avaliados pelo critério GES em ciclos de mulheres submetidas à fertilização in vitro (FIV). Métodos: Um estudo transversal foi realizado. Avaliaram-se setenta e três mulheres com idade ≤ 39 anos, submetidas à FIV pelas seguintes causas: fator tubário, fator masculino e/ou endometriose, com ambos os ovários e níveis hormonais de TSH, FSH e PRL dentro dos valores de referência. Não foram elegíveis ao estudo mulheres com síndrome de hiperestimulação ovariana no ciclo avaliado, com doença auto-imune, com síndrome dos ovários policísticos, com luteinização precoce e/ou endometrioma. Realizou-se a mensuração de sHLA-G por ELISA no FF de um pool de folículos puncionados das mulheres submetidas à indução de ovulação para a FIV. Os embriões obtidos após fertilização foram classificados segundo o critério GES. As outras variáveis necessárias foram transcritas dos prontuários das pacientes participantes no estudo. Resultados: A associação entre as concentrações de sHLA-G e o escore médio dos embriões gerados não foi observada. Observou-se uma sensibilidade aumentada do teste ELISA, quando comprada aos estudos da literatura, com detecção em mais de 98% das amostras analisadas. Foi encontrada uma associação significativa entre o AMH e a idade (r=-0,38; p=0,003), entre AMH e total de oócitos ( r=0,53; p<0,05) e idade e total de oócitos (r= -0,31, p=0,009). A mensuração de sHLA-G em mulheres com endometriose e sem endometriose (fator tubário e masculino) não mostrou diferença significativa (p=0,57). Conclusão: Não houve associação entre a qualidade embrionária e dosagens de sHLA-G. Diante destes resultados, ainda está controverso o uso de sHLA-G mensurado em FF como marcador prognóstico de qualidade de embrião embrionária avaliada pelo critério GES. Os resultados encontrados referentes ao AMH corroboram com a literatura.

Background: The prevalence of infertility rate in…

Advisors/Committee Members: Cunha Filho, João Sabino Lahorgue da.

Subjects/Keywords: sHLA-G; Endometriose; GES; Fertilização in vitro; Hormônio anti-mülleriano; Embryo quality; Estruturas embrionárias; Follicular fluid; Endometriosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bezerra, G. P. (2014). Associação entre as concentrações de antígenos leucocitários humanos-G em fluído folicular e a qualidade do embrião avaliada pelo critério de escore embrionário graduado em ciclos de fertilização in vitro. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/127400

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bezerra, Glícia Pinheiro. “Associação entre as concentrações de antígenos leucocitários humanos-G em fluído folicular e a qualidade do embrião avaliada pelo critério de escore embrionário graduado em ciclos de fertilização in vitro.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed March 06, 2021. http://hdl.handle.net/10183/127400.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bezerra, Glícia Pinheiro. “Associação entre as concentrações de antígenos leucocitários humanos-G em fluído folicular e a qualidade do embrião avaliada pelo critério de escore embrionário graduado em ciclos de fertilização in vitro.” 2014. Web. 06 Mar 2021.

Vancouver:

Bezerra GP. Associação entre as concentrações de antígenos leucocitários humanos-G em fluído folicular e a qualidade do embrião avaliada pelo critério de escore embrionário graduado em ciclos de fertilização in vitro. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10183/127400.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bezerra GP. Associação entre as concentrações de antígenos leucocitários humanos-G em fluído folicular e a qualidade do embrião avaliada pelo critério de escore embrionário graduado em ciclos de fertilização in vitro. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/127400

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Marques, Mariana Groke. Efeito da tensão de oxigênio e do meio na maturação oocitária in vitro e sua influência no desenvolvimento embrionário em suínos.

Degree: PhD, Reprodução Animal, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-14012010-131425/ ;

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O objetivo deste estudo foi avaliar a eficiência da baixa tensão de oxigênio (5% de CO2; 5% de O2 e 90% de N2) na maturação… (more)

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O objetivo deste estudo foi avaliar a eficiência da baixa tensão de oxigênio (5% de CO2; 5% de O2 e 90% de N2) na maturação oocitária in vitro em meio quimicamente definido (0,1% de PVA) ou suplementado com 10 % de PFF. Foram avaliados a migração dos grânulos corticais, a quantificação e distribuição da proteína HSP70, a maturação nuclear, as concentrações intracelulares da glutationa reduzida, a avaliação dos índices de penetração espermática e o desenvolvimento e qualidade de embriões fecundados in vitro em oócitos antes da maturação (0 hora) e após a maturação in vitro nos diferentes tratamentos (atmosfera = 5 ou 20% de O2 e suplementação do meio de maturação = 0,1% de PVA ou 10% de PFF). Para verificar a influência da atmosfera e da suplementação do meio no sistema de maturação foram avaliados o ciclo celular das células do cumulus, o número de células do cumulus por oócitos, as concentrações intracelulares de glutationa reduzida (GSH) das células do cumulus, as concentrações de TBARS no meio de maturação e a resistência das células do cumulus ao estresse oxidativo. Avaliações quanto às concentrações de GSH e HSP70 foram realizadas em oócitos in vivo, visando comparar a eficiência do sistema in vitro. Para a maioria dos parâmetros avaliados não houve interação entre a atmosfera e a suplementação do meio de maturação, indicando que o efeito da atmosfera independe do meio utilizado. Foi observado que em atmosfera com baixa tensão de O2 houve diminuição da concentração de glutationa nas células do cumulus, do índice de clivagem e do número total de blastômeros dos embriões e houve aumento do número de células por oócito após o período de maturação in vitro. A suplementação do meio de maturação com 0,1% de PVA diminuiu os índices de migração dos grânulos corticais, contudo não alterou os índices de penetração espermática. Os oócitos do grupo 0 hora apresentaram índices maiores de HSP70 do que cada um dos demais grupos, indicando provável diminuição dos estoques de HSP70 durante o período de maturação em todos os grupos de maturação in vitro. As células do cumulus provenientes de oócitos maturados em meio suplementado com 0,1% de PVA apresentaram maior número de células na fase S e G2/M do que o grupo 0 hora, entretanto isso não refletiu em aumento no número de células por oócito após a maturação. Este trabalho permite concluir que o uso de baixa tensão de oxigênio não melhora as condições do sistema de maturação oocitária in vitro em suínos, sendo que o efeito do uso desta atmosfera é, na maioria das variáveis avaliadas, independente do tipo de suplementação que se utiliza no meio de maturação in vitro.

The aim of this study was to evaluate the efficiency of low oxygen tension (5% CO2, 5% O2 and 90% N2) on in vitro oocyte maturation using defined media (0.1% PVA) or supplemented with 10% PFF. To achieve this goal, oocytes were evaluated prior to in vitro maturation (0 hour) and after in vitro maturation on different treatments (atmosphere = 5 or 20% O2 and media supplementation = 0.1% PVA or 10% PFF) for cortical…

Advisors/Committee Members: Visintin, José Antonio.

Subjects/Keywords: Álcool polivinílico; Fluido folicular; Follicular fluid; Maturação oocitária; Oocyte maturation; Oxygen tension; Polyvinyl alcohol; Porcine; Suínos; Tensão de oxigênio

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APA (6^th Edition):

Marques, M. G. (2009). Efeito da tensão de oxigênio e do meio na maturação oocitária in vitro e sua influência no desenvolvimento embrionário em suínos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-14012010-131425/ ;

Chicago Manual of Style (16^th Edition):

Marques, Mariana Groke. “Efeito da tensão de oxigênio e do meio na maturação oocitária in vitro e sua influência no desenvolvimento embrionário em suínos.” 2009. Doctoral Dissertation, University of São Paulo. Accessed March 06, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-14012010-131425/ ;.

MLA Handbook (7^th Edition):

Marques, Mariana Groke. “Efeito da tensão de oxigênio e do meio na maturação oocitária in vitro e sua influência no desenvolvimento embrionário em suínos.” 2009. Web. 06 Mar 2021.

Vancouver:

Marques MG. Efeito da tensão de oxigênio e do meio na maturação oocitária in vitro e sua influência no desenvolvimento embrionário em suínos. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Mar 06]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-14012010-131425/ ;.

Council of Science Editors:

Marques MG. Efeito da tensão de oxigênio e do meio na maturação oocitária in vitro e sua influência no desenvolvimento embrionário em suínos. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-14012010-131425/ ;

University of Michigan

13. Benedict, Merle D. Secondhand Tobacco Smoke Exposure and its Effects among Women Undergoing In Vitro Fertilization.

Degree: PhD, Environmental Health Sciences, 2011, University of Michigan

URL: http://hdl.handle.net/2027.42/86508

► Infertility and early pregnancy loss are prevalent in the United States and worldwide, and a large proportion of non-tobacco users are exposed to secondhand tobacco… (more)

▼ Infertility and early pregnancy loss are prevalent in the United States and worldwide, and a large proportion of non-tobacco users are exposed to secondhand tobacco smoke (STS). In one of the only previous studies of early pregnancy that utilized biomarkers of STS exposure, no relationship was observed between urinary cotinine and in vitro fertilization (IVF) outcomes. However, we hypothesize that urine may not be the most relevant sample media within which to measure biomarkers for a study of early pregnancy, and that the lack of an association may have been due to a large degree exposure measurement error. In the first chapter of this dissertation we report a weak rank-order relationship (Spearman r <0.2) and poor exposure category agreement between cotinine concentrations measured in urine and the potentially more biologically-relevant follicular fluid (FF) among 255 women undergoing IVF. We also observed fair reliability (ICC=0.42-0.52) in FF cotinine concentrations from 415 women undergoing multiple IVF cycles. In the second chapter, we report a significant increase in the risk of embryo implantation failure among women exposed to STS compared to those who were unexposed (Odds Ratio[OR]=1.52; 95% confidence interval[CI]=1.20-1.92; Risk Ratio[RR]=1.17; 95% CI=1.10-1.25) based on cotinine measures in FF collected during 3,270 IVF cycles from 1,909 nonsmoking women. We also found a significant decrease in the odds for a livebirth among STS-exposed women (OR=0.75; 95% CI=0.57-0.99; RR=0.81; 95% CI=0.66-0.99). In the third chapter, among 337 of the women in the study, we report a significant increase in circulating prolactin (p=0.03), but no change in circulating TSH, among STS-exposed women. These findings are likely of great public health significance due to continued widespread STS exposure, the rapidly increasing number of couples utilizing IVF, and the potential relationship between elevated prolactin concentrations and a range of downstream adverse health effects. In addition, FF cotinine concentrations, if available, may be desired as a biomarker of low-level tobacco smoke exposure over urinary cotinine in studies of early reproduction. Cotinine measured in FF samples collected at each IVF cycle may be needed to most accurately represent STS exposure in studies utilizing outcome data from multiple cycles. Advisors/Committee Members: Meeker, John D. (committee member), Harris, Craig (committee member), Tsodikov, Alexander (committee member), Vahratian, Anjel M. (committee member).

Subjects/Keywords: Secondhand Smoke; Environmental Tobacco Smoke; In Vitro Fertilization; Follicular Fluid; Cotinine; Implantation Failure; Obstetrics and Gynecology; Public Health; Health Sciences

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APA (6^th Edition):

Benedict, M. D. (2011). Secondhand Tobacco Smoke Exposure and its Effects among Women Undergoing In Vitro Fertilization. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/86508

Chicago Manual of Style (16^th Edition):

Benedict, Merle D. “Secondhand Tobacco Smoke Exposure and its Effects among Women Undergoing In Vitro Fertilization.” 2011. Doctoral Dissertation, University of Michigan. Accessed March 06, 2021. http://hdl.handle.net/2027.42/86508.

MLA Handbook (7^th Edition):

Benedict, Merle D. “Secondhand Tobacco Smoke Exposure and its Effects among Women Undergoing In Vitro Fertilization.” 2011. Web. 06 Mar 2021.

Vancouver:

Benedict MD. Secondhand Tobacco Smoke Exposure and its Effects among Women Undergoing In Vitro Fertilization. [Internet] [Doctoral dissertation]. University of Michigan; 2011. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2027.42/86508.

Council of Science Editors:

Benedict MD. Secondhand Tobacco Smoke Exposure and its Effects among Women Undergoing In Vitro Fertilization. [Doctoral Dissertation]. University of Michigan; 2011. Available from: http://hdl.handle.net/2027.42/86508

14. Scalici, Elodie. Les acides nucléiques circulants : biomarqueurs d'intérêt en Assistance Médicale à la Procréation : Circulating nucleic acids : innovative biomarkers in Assisted Reproductive Technology.

Degree: Docteur es, Biologie Santé, 2015, Montpellier

URL: http://www.theses.fr/2015MONTT031

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Au cours de ces dernières années, l’utilisation des acides nucléiques circulants comme outils diagnostiques et/ou pronostiques en cancérologie a largement été documentée. Récemment, le développement… (more)

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Au cours de ces dernières années, l’utilisation des acides nucléiques circulants comme outils diagnostiques et/ou pronostiques en cancérologie a largement été documentée. Récemment, le développement du diagnostic prénatal non-invasif a également révélé l’intérêt grandissant de ces biomarqueurs en gynécologie-obstétrique. Les acides nucléiques circulants ou extracellulaires ont la particularité d’être facilement détectables dans les fluides biologiques de l’organisme et sont de deux types: (1) l’ADN libre, courts ou longs fragments d’ADN provenant des processus apoptotiques et/ou nécrotiques cellulaires (2) les microARNs, petites séquences d’ARN non codantes, qui régulent l’expression des gènes en interférant avec leurs ARNm cibles. Sachant qu’il a été démontré que le taux d’ADN libre circulant est anormalement élevé dans certaines conditions pathologiques et que les microARNs sont impliqués dans la régulation de nombreux processus biologiques tels que la folliculogénèse et la stéroïdogénèse, ces deux types d’acides nucléiques pourraient alors constituer des biomarqueurs d’intérêt dans le domaine de l’Assistance Médicale à la Procréation. Dans ce travail de thèse, nous avons à la fois mesuré le taux d’ADN libre et analysé les profils d’expression de plusieurs microARNs d’intérêt par PCR quantitative, dans le liquide folliculaire (LF) de patientes prises en charge en fécondation in vitro (FIV). Nous avons observé des taux d’ADN libre significativement élevés ainsi que des profils d’expression de microARNs spécifiques dans le LF de patientes présentant des anomalies de la réserve ovarienne (telles que le syndrome des ovaires micropolykystiques ou une faible réserve ovarienne). Nous avons ensuite évalué le potentiel des acides nucléiques circulants en tant biomarqueurs prédictifs des résultats en FIV. Nous avons démontré que le taux d’ADN libre intra-folliculaire et l’expression de certains microARNs étaient significativement associés à la qualité des embryons obtenus in vitro ainsi qu’à la survenue d’une grossesse clinique. Les acides nucléiques circulants offrent donc de nouvelles perspectives à la fois d’un point de vue diagnostique et pronostique dans la prise en charge de l’infertilité humaine.

During the last years, the use of circulating nucleic acids as diagnostic and/or prognostic tools in cancerology was widely documented. The recent development of non-invasive prenatal testing also reveals the growing interest of these biomarkers in obstetrics and gynecology. The circulating or extracellular nucleic acids have for particularity to be easily detectable in the biological fluids of the body and there are two types: (1) Cell-free DNA (cfDNA), short or long DNA fragments resulting from cellular apoptosis and/or necrosis (2) microRNAs (miRNAs), small non-coding RNA sequences, which regulate gene expression by interfering with their mRNA targets. Since it was demonstrated that cfDNA level is abnormally increased in some pathological conditions and miRNAs are involved in the regulation of several biological…

Advisors/Committee Members: Hamamah, Samir (thesis director).

Subjects/Keywords: MicroARN; ADN libre; Liquide folliculaire; Réserve ovarienne; Qualité embryonnaire; Amp; MicroRNA; Cell-Free DNA; Follicular fluid; Ovarian reserve; Embryo quality; Art

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APA (6^th Edition):

Scalici, E. (2015). Les acides nucléiques circulants : biomarqueurs d'intérêt en Assistance Médicale à la Procréation : Circulating nucleic acids : innovative biomarkers in Assisted Reproductive Technology. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2015MONTT031

Chicago Manual of Style (16^th Edition):

Scalici, Elodie. “Les acides nucléiques circulants : biomarqueurs d'intérêt en Assistance Médicale à la Procréation : Circulating nucleic acids : innovative biomarkers in Assisted Reproductive Technology.” 2015. Doctoral Dissertation, Montpellier. Accessed March 06, 2021. http://www.theses.fr/2015MONTT031.

MLA Handbook (7^th Edition):

Scalici, Elodie. “Les acides nucléiques circulants : biomarqueurs d'intérêt en Assistance Médicale à la Procréation : Circulating nucleic acids : innovative biomarkers in Assisted Reproductive Technology.” 2015. Web. 06 Mar 2021.

Vancouver:

Scalici E. Les acides nucléiques circulants : biomarqueurs d'intérêt en Assistance Médicale à la Procréation : Circulating nucleic acids : innovative biomarkers in Assisted Reproductive Technology. [Internet] [Doctoral dissertation]. Montpellier; 2015. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2015MONTT031.

Council of Science Editors:

Scalici E. Les acides nucléiques circulants : biomarqueurs d'intérêt en Assistance Médicale à la Procréation : Circulating nucleic acids : innovative biomarkers in Assisted Reproductive Technology. [Doctoral Dissertation]. Montpellier; 2015. Available from: http://www.theses.fr/2015MONTT031

Louisiana State University

15. Klumpp, Angela Marie. The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development.

Degree: MS, Animal Sciences, 2003, Louisiana State University

URL: etd-03242004-175322 ; https://digitalcommons.lsu.edu/gradschool_theses/2563

► The objective of Experiment 1 was to determine the effect of bovine follicular fluid (bFF) on nuclear maturation. Treatment A (Control) oocytes were stained with… (more)

▼ The objective of Experiment 1 was to determine the effect of bovine follicular fluid (bFF) on nuclear maturation. Treatment A (Control) oocytes were stained with Hoechst-33342 immediately after aspiration from follicles, whereas, oocytes in Treatment B were held in bFF for 12 hours at 38¢ªC and then stained to determine nuclear status. No significant difference was detected between treatment groups. Results indicate that bFF inhibits resumption of meiosis. The objective of Experiment 2 was to determine the effect of bFF on embryonic development. Oocytes in Treatment A (Control) were placed into in vitro maturation (IVM) for 22 hours followed by in vitro fertilization (IVF). Oocytes in Treatment B were held in bFF for 12 hours at 22¢ªC, followed by IVM and then subjected to IVF. Significantly more (P<0.0001) oocytes cleaved, developed into blastocysts and hatched in Treatment A compared with Treatment B. Results indicate that a 12-hour holding period in bFF does not promote normal embryonic development. The objective of Experiment 3 was to determine the effect of decreased time and concentration of bFF on embryonic development. Treatment A (Control) oocytes were placed into IVM followed by IVF. Oocytes in Treatment B were held in bFF, oocytes in Treatment C were held in Lactated Ringer¡¯s Solution (LRS) and oocytes in Treatment D were held in a combination of bFF and LRS for 6 hours at 22¢ªC, followed by IVM then by IVF. No significant difference was detected between Treatments A and B when analyzing cleavage, blastocyst formation and hatching rates. However, significantly fewer (P<0.0001) embryos reached these stages of development in Treatments C and D. Nevertheless, there were significantly more embryos that developed to the blastocyst stage in Treatment D compared with Treatment C. Decreasing the amount of time that oocytes were held in bFF proved to be beneficial in supporting in vitro embryo production (IVP). These findings could be advantageous when attempting to rescue valuable gametes from deceased females.

Subjects/Keywords: bovine; in vitro fertilization; in vitro maturation; follicular fluid

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APA (6^th Edition):

Klumpp, A. M. (2003). The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development. (Masters Thesis). Louisiana State University. Retrieved from etd-03242004-175322 ; https://digitalcommons.lsu.edu/gradschool_theses/2563

Chicago Manual of Style (16^th Edition):

Klumpp, Angela Marie. “The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development.” 2003. Masters Thesis, Louisiana State University. Accessed March 06, 2021. etd-03242004-175322 ; https://digitalcommons.lsu.edu/gradschool_theses/2563.

MLA Handbook (7^th Edition):

Klumpp, Angela Marie. “The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development.” 2003. Web. 06 Mar 2021.

Vancouver:

Klumpp AM. The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development. [Internet] [Masters thesis]. Louisiana State University; 2003. [cited 2021 Mar 06]. Available from: etd-03242004-175322 ; https://digitalcommons.lsu.edu/gradschool_theses/2563.

Council of Science Editors:

Klumpp AM. The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development. [Masters Thesis]. Louisiana State University; 2003. Available from: etd-03242004-175322 ; https://digitalcommons.lsu.edu/gradschool_theses/2563

16. Jarrett, Selene. Altered protein and fatty acid composition of porcine follicular fluid due to a high fibre diet and the subsequent effects on oocyte maturation.

Degree: PhD, 2018, University of Edinburgh

URL: http://hdl.handle.net/1842/33086

► Background Ovarian follicular fluid serves as the microenvironment for a maturing oocyte prior to ovulation. Previous studies have shown that gilts fed a high fibre… (more)

▼ Background Ovarian follicular fluid serves as the microenvironment for a maturing oocyte prior to ovulation. Previous studies have shown that gilts fed a high fibre (HF) diet before ovulation have improved fertility compared to gilts fed a control (C) diet, including a higher proportion of metaphase II oocytes following in vitro maturation (IVM). Hypothesis The molecular composition of porcine follicular fluid (pFF) was altered by the diet and that these alterations conferred the fertility benefits. Aims The aim of this study was to compare the protein composition of pFF from pigs fed a control diet with pFF of pigs fed a high fibre diet, to identify whether a high fibre diet fed to pigs during their oestrous cycle altered the composition of pFF. Additionally, the pFF of fertile animals was compared with the pFF of non-fertile animals to identify whether pFF composition was associated with fertility; fertile animals produced an embryo following in vitro fertilisation (IVF). Differences in the molecular composition were to be used to ascertain the potential underlying mechanism(s) involved in dietary induced improvements to oocyte maturation. Results The protein composition of pooled pFF from 12 HF-pigs and 12 C-pigs was compared by liquid chromatography tandem mass spectrometry (LC-MS/MS). Additionally, within each dietary group, the composition of pooled pFF from pigs whose oocytes produced blastocysts following in vitro fertilisation (C-Bl and HF-Bl) was compared with pFF from pigs whose oocytes did not produce blastocysts (C-No and HF-No respectively; n=6 per group). These proteomic analyses identified differentially expressed proteins, associated with several canonical pathways including acute phase response signalling, complement system and LXR/RXR activation, as determined by Ingenuity Pathway Analysis. Quantitative western blots revealed the differential expression of candidates associated with these canonical pathways. Plasminogen expression was lower (P≤0.05) in pFF of HF-pigs compared to pFF of C-pigs. In pFF from C-Bl gilts, apolipoprotein A4 (P≤0.01) and apolipoprotein M (P≤0.05) expression were higher compared to pFF from C-No gilts. Plasmin expression was lower (P≤0.05) in pFF from HF-Bl gilts compared to pFF from C-Bl gilts. Due to the interest in the differentially expressed apolipoproteins (involved in cholesterol and lipid efflux), a targeted metabolomic analysis was carried out to measure the concentration of nine fatty acids (FAs) in pFF of individual pigs in C-No, C-Bl, HF-No, HF-Bl groups (n=6 per group); adrenic, arachadonic, arachidic, dihomo- γ-linolenic, docosapentaenoic, erucic, linoleic, palmitoleic and oleic acids were measured by LC-MS/MS. The analysis revealed the lower concentration of linoleic acid (LA, p≤0.05) and higher concentration of erucic acid (P≤0.05) in HF-pFF compared to C-pFF. Following the results of the targeted metabolomic analysis, cumulus-oocytecomplexes (COCs) were matured in TCM 199 medium supplemented with 0 (No-LA), 50, 100 or 200 μM LA for 44 hours (n = 320 per…

Subjects/Keywords: 636.089; ovarian follicular fluid; control-fed pigs; high fibre-fed pigs; plasminogen; plasmin; apolipoprotein A4; apolipoprotein M; fat regulation; linoleic acid

10 more images…

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APA (6^th Edition):

Jarrett, S. (2018). Altered protein and fatty acid composition of porcine follicular fluid due to a high fibre diet and the subsequent effects on oocyte maturation. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/33086

Chicago Manual of Style (16^th Edition):

Jarrett, Selene. “Altered protein and fatty acid composition of porcine follicular fluid due to a high fibre diet and the subsequent effects on oocyte maturation.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed March 06, 2021. http://hdl.handle.net/1842/33086.

MLA Handbook (7^th Edition):

Jarrett, Selene. “Altered protein and fatty acid composition of porcine follicular fluid due to a high fibre diet and the subsequent effects on oocyte maturation.” 2018. Web. 06 Mar 2021.

Vancouver:

Jarrett S. Altered protein and fatty acid composition of porcine follicular fluid due to a high fibre diet and the subsequent effects on oocyte maturation. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1842/33086.

Council of Science Editors:

Jarrett S. Altered protein and fatty acid composition of porcine follicular fluid due to a high fibre diet and the subsequent effects on oocyte maturation. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/33086

University of Otago

17. Alsop, Toni-Ann. Epithelial ion transport and luminal fluid composition in the vaginal cul-de-sac of the Australian brushtail possum Trichosurus vulpecula.

Degree: 2013, University of Otago

URL: http://hdl.handle.net/10523/4267

► The brushtail possum (Trichosurus vulpecula) is an introduced pest in New Zealand and there is interest in developing alternatives to existing toxin-based controls. The vaginal… (more)

▼ The brushtail possum (Trichosurus vulpecula) is an introduced pest in New Zealand and there is interest in developing alternatives to existing toxin-based controls. The vaginal cul-de-sac is a structure of unknown function that is unique to marsupials. During the follicular stage of oestrous, the epithelium lining the cul-de-sac proliferates and a mucus-rich luminal fluid is secreted. Following ovulation, the epithelium regresses and the fluid disappears. Identifying the epithelial transport processes mediating the cyclic changes in cul-de-sac fluid may assist with the development of a possum-specific contraceptive. The Ussing short-circuit (Isc) technique and measurements of transcript and protein expression of the epithelial Na+ channel (ENaC) were used to determine if variations in electrogenic Na+ transport are associated with cul-de-sac fluid disappearance. Spontaneous Isc (<20 µA cm-2 during anoestrus, 60-80 µA cm-2 in cycling animals) was inhibited by serosal ouabain. Mucosal amiloride (10 µmol L-1), an inhibitor of ENaC, had little effect on follicular Isc but reduced luteal Isc by ≈35%. This amiloride-sensitive Isc was dependent on mucosal Na+ and the IC50-amiloride (0.95 µmol L-1) was consistent with ENaC-mediated Na+ absorption. αENaC mRNA expression was low in anoestrus, but increased to similar levels in the follicular and luteal phases. However, in follicular animals αENaC immunoreactivity in epithelial cells was evident throughout the cytoplasm, but was restricted to the apical pole of cells from luteal animals. These data suggest that increased Na+ absorption contributes to fluid absorption during the luteal phase and is regulated by insertion of ENaC into the apical membrane of epithelial cells. In the follicular stage, 79±6% of the amiloride-insensitive Isc was inhibited by the anion channel blocker 5-Nitro-2-(3-phenylpropylamino)-benzoic-acid (NPPB, 100 µmol L-1 mucosal). This NPPB sensitive current (INPPB) was reduced when both Cl- and HCO3- were substituted from the serosal solution, suggesting that HCO3- and Cl- secretion contribute to the formation of cul-de-sac fluid. In Cl – free conditions, the carbonic anhydrase (CA) blocker acetazolamide (1 mmol L-1 mucosal and serosal) inhibited 31±6% of the Isc, whereas in HCO3-/CO2-free conditions the Na+/K+/2Cl- cotransporter (NKCC1) blocker bumetanide (10 µmol L-1 serosal) inhibited 28±6% of Isc. Collectively, these data indicate that HCO3- and Cl- secretion can occur in the follicular phase, and are facilitated by CA and NKCC1, respectively. CA mediated HCO3- secretion may involve an apical Na+/H+ exchanger (NHE) as the INPPB of cul-de-sac tissue was dependent on mucosal Na+, and mucosal 5-(N-ethyl-N-isopropyl)-amiloride (EIPA, 200 µmol L-1) an NHE inhibitor, blocked 35.8±5% of the spontaneous Isc. RT-PCR results indicate that NHE2 may be expressed. Cul-de-sac fluid from follicular animals had low Cl- activity (7.7±9.4 mmol L-1) and [Na+] (25.3±17.2 mmol L-1), while [HCO3-] (93.3±83.4 mmol L-1) and [Ca2+] (75±28.8 mmol L-1) were high. However,… Advisors/Committee Members: Butt, Grant (advisor).

Subjects/Keywords: Epithelial; Transport; Possum; Trichosurus vulpecula; ENaC; Oestrous cycle; vaginal cul-de-sac; Luteal stage; Follicular stage; Short-circuit current; Ussing chamber; Luminal fluid; Luminal fluid composition; NHE; Bicarbonate; Calcium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alsop, T. (2013). Epithelial ion transport and luminal fluid composition in the vaginal cul-de-sac of the Australian brushtail possum Trichosurus vulpecula. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/4267

Chicago Manual of Style (16^th Edition):

Alsop, Toni-Ann. “Epithelial ion transport and luminal fluid composition in the vaginal cul-de-sac of the Australian brushtail possum Trichosurus vulpecula. ” 2013. Doctoral Dissertation, University of Otago. Accessed March 06, 2021. http://hdl.handle.net/10523/4267.

MLA Handbook (7^th Edition):

Alsop, Toni-Ann. “Epithelial ion transport and luminal fluid composition in the vaginal cul-de-sac of the Australian brushtail possum Trichosurus vulpecula. ” 2013. Web. 06 Mar 2021.

Vancouver:

Alsop T. Epithelial ion transport and luminal fluid composition in the vaginal cul-de-sac of the Australian brushtail possum Trichosurus vulpecula. [Internet] [Doctoral dissertation]. University of Otago; 2013. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10523/4267.

Council of Science Editors:

Alsop T. Epithelial ion transport and luminal fluid composition in the vaginal cul-de-sac of the Australian brushtail possum Trichosurus vulpecula. [Doctoral Dissertation]. University of Otago; 2013. Available from: http://hdl.handle.net/10523/4267

18. Bitounis, Dimitrios. Etude de la charge nanoparticulaire de divers prélèvements biologiques : Study of the nanoparticulate load of various human biological samples.

Degree: Docteur es, Sciences de la vie et de la sante, 2017, Lyon

URL: http://www.theses.fr/2017LYSES017

►

L'analyse des nanoparticules dans les échantillons cliniques est une entreprise scientifique redoutable puisqu'il n'existe pas de procédures capables de faire face à la complexité des… (more)

▼

L'analyse des nanoparticules dans les échantillons cliniques est une entreprise scientifique redoutable puisqu'il n'existe pas de procédures capables de faire face à la complexité des matrices biologiques. L'hypothèse centrale de ce travail est que la présence accrue de nanoparticules d'oxyde de métal et de métal dans le lavage pulmonaire et les fluides reproducteurs peut être associée à une maladie pulmonaire interstitielle idiopathique et à une baisse de la fertilité féminine et masculine, respectivement. Les procédures d'extraction développées dans le cadre de cette thèse ont permis de mesurer la distribution de la taille hydrodynamique des nanoparticules d'or par DLS et leur quantification par ICP-OES. En raison des rendements d'extraction obtenus et de la ségrégation réussie du bruit biologique, les nanoparticules d'or extraites pourraient être observées de manière représentative à l'échelle nanométrique. Plus précisément, l'ESB pourrait détecter les noyaux denses de particules d'or à faible grossissement, et MET et MEB pourraient être utilisés pour résoudre le diamètre et la morphologie des feret des particules, respectivement. Enfin, la corona biomoleculaire dure de la nanoparticule d'or extraite à partir de lavages pulmonaires pourrait être sondée avec AFM, AES et XPS pour acquérir des informations sur sa morphologie, composition élémentaire et chimie, respectivement. L'application de méthodologies développées sur les patients n'a pas renvoyé de données analytiques qui pourraient associer de manière positive et significative une teneur élevée en particules avec une probabilité accrue de maladies pulmonaire interstitielle idiopathiques ou avec oligospermie.

The quantitative and qualitative analysis of nanoparticles in human biological samples is a daunting scientific endeavour as there are no technical procedures capable to cope with the complexity of biological matrices. The central hypothesis of this work is that the increased presence of metal and metal oxide nanoparticles in pulmonary lavage and reproductive fluids may be associated with idiopathic interstitial lung disease and declining female and male fertility, respectively. The developed extraction procedures in the context of this thesis allowed the measuring of the the hydrodynamic size distribution of gold nanoparticles by DLS and their quantitation my means of ICP-OES. Due to the achieved extraction yields and the successful segregation of biological noise, extracted gold nanoparticles could be representatively observed at the nanoscale. Specifically, BSE could detect the dense gold particle cores at low magnification, and TEM and FESEM could be used to resolve the particles’ Feret diameter and morphology, respectively. Finally, the hard biomolecular corona of extracted gold nanoparticle from pulmonary lavages could be probed with AFM, AES, and XPS to acquire information on its morphology, elemental composition, and chemistry, respectively. The application of developed methodologies on patients did not return analytical data that could…

Advisors/Committee Members: Cottier-Jouffre, Michèle (thesis director), Pourchez, Jérémie (thesis director).

Subjects/Keywords: Nanoparticules; Détection; Analyse; Quantitative; Qualitative; Lavage broncho-alvéolaire; Liquide séminal; Liquide folliculaire; Nanoparticles; Detection; Analysis; Quantitative; Qualitative; Bronchoalveolar lavage; Seminal fluid; Follicular fluid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bitounis, D. (2017). Etude de la charge nanoparticulaire de divers prélèvements biologiques : Study of the nanoparticulate load of various human biological samples. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2017LYSES017

Chicago Manual of Style (16^th Edition):

Bitounis, Dimitrios. “Etude de la charge nanoparticulaire de divers prélèvements biologiques : Study of the nanoparticulate load of various human biological samples.” 2017. Doctoral Dissertation, Lyon. Accessed March 06, 2021. http://www.theses.fr/2017LYSES017.

MLA Handbook (7^th Edition):

Bitounis, Dimitrios. “Etude de la charge nanoparticulaire de divers prélèvements biologiques : Study of the nanoparticulate load of various human biological samples.” 2017. Web. 06 Mar 2021.

Vancouver:

Bitounis D. Etude de la charge nanoparticulaire de divers prélèvements biologiques : Study of the nanoparticulate load of various human biological samples. [Internet] [Doctoral dissertation]. Lyon; 2017. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2017LYSES017.

Council of Science Editors:

Bitounis D. Etude de la charge nanoparticulaire de divers prélèvements biologiques : Study of the nanoparticulate load of various human biological samples. [Doctoral Dissertation]. Lyon; 2017. Available from: http://www.theses.fr/2017LYSES017

19. Karagiannis, Dimitrios. Συσχέτιση της μεσογειακής δίαιτας με τη γονιμότητα: μελέτη σε δείγμα υπογόνιμων ζευγαριών που ακολουθούν πρόγραμμα εξωσωματικής γονιμοποίησης.

Degree: 2020, Harokopio University; Χαροκόπειο Πανεπιστήμιο

URL: http://hdl.handle.net/10442/hedi/47588

► Background & Objective: Infertility affects one in every six couples and one out of ten couples may seek fertility treatment such as ovulation induction and… (more)

▼ Background & Objective: Infertility affects one in every six couples and one out of ten couples may seek fertility treatment such as ovulation induction and assisted reproductive technologies (ARTs). Modifiable lifestyle factors, including diet, exercise and stress affect reproductive performance, also during assisted reproduction. With respect to diet, several dietary factors (i.e. micro-, macro-nutrients and individual food groups) have been associated with the risk of subfertility; however, more information is needed on the role of dietary factors in reproductive health. A more comprehensive approach to investigate the associations between dietary factors and health outcomes is to assess the impact of the overall diet rather than separate foods or nutrients. However, evidence concerning the association between dietary patterns, such as the Mediterranean diet (MD), and fertility is scarce. Therefore, the aim of the present doctoral dissertation was to investigate the relationship between preconception adherence to the MD and assisted reproduction performance. In particular, the study aimed at investigating associations between adherence to the MD, semen quality parameters, and in vitro fertilization (IVF) clinical outcomes among couples attempting fertility. Moreover, given the fact that follicular fluid (FF) composition influence ARTs outcome, to assess the potential interrelationship of FF biomarkers (i.e. activin-A inhibin-B, follistatin, adiponectin and FF antioxidant capacity) with dietary habits and IVF outcomes. Methodological Design: This prospective cohort study included 244 infertile couples undergoing a first IVF treatment in an Assisted Conception Unit in Athens, Greece, between November 2013 and September 2016. At enrollment, both men and women completed detailed questionnaires requesting information on demographics, reproductive health, medical history and lifestyle factors, and anthropometric measurements were taken. Diet was assessed before the IVF treatment via a validated food-frequency questionnaire. Adherence to the MD was assessed through the MedDietScore (range: 0–55), with higher scores indicating greater adherence. Semen quality was evaluated according to World Health Organization 2010. Intermediate IVF outcomes (oocyte yield, fertilization rate and embryo quality measures) and clinical endpoints (implantation, clinical pregnancy and live birth) were abstracted from electronic medical records. Biochemical analyses were performed using standard procedures in FF samples collected from the dominant follicle of 86 women. Associations between MedDietScore and semen quality parameters, as well as between MedDietScore, IVF treatment outcomes and FF biomarkers were tested in multivariable models adjusted for several potential confounders. Results: Compared to men with the highest adherence to the MD (highest tertile of the MedDietScore), a higher percentage of men adhering less closely to the MD (lowest tertile of the MedDietScore) exhibited below the WHO reference values for sperm concentration…

Subjects/Keywords: Μεσογειακή δίαιτα; Υπογονιμότητα; Εξωσωματική γονιμοποίηση; Ωοθυλακικό υγρό; Ζώσα γέννηση; Ακτιβίνη Α; Φολιστατίνη; Mediterannean diet; In vitro fertilization; Infertility; Semen analysis; Follicular fluid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Karagiannis, D. (2020). Συσχέτιση της μεσογειακής δίαιτας με τη γονιμότητα: μελέτη σε δείγμα υπογόνιμων ζευγαριών που ακολουθούν πρόγραμμα εξωσωματικής γονιμοποίησης. (Thesis). Harokopio University; Χαροκόπειο Πανεπιστήμιο. Retrieved from http://hdl.handle.net/10442/hedi/47588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Karagiannis, Dimitrios. “Συσχέτιση της μεσογειακής δίαιτας με τη γονιμότητα: μελέτη σε δείγμα υπογόνιμων ζευγαριών που ακολουθούν πρόγραμμα εξωσωματικής γονιμοποίησης.” 2020. Thesis, Harokopio University; Χαροκόπειο Πανεπιστήμιο. Accessed March 06, 2021. http://hdl.handle.net/10442/hedi/47588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Karagiannis, Dimitrios. “Συσχέτιση της μεσογειακής δίαιτας με τη γονιμότητα: μελέτη σε δείγμα υπογόνιμων ζευγαριών που ακολουθούν πρόγραμμα εξωσωματικής γονιμοποίησης.” 2020. Web. 06 Mar 2021.

Vancouver:

Karagiannis D. Συσχέτιση της μεσογειακής δίαιτας με τη γονιμότητα: μελέτη σε δείγμα υπογόνιμων ζευγαριών που ακολουθούν πρόγραμμα εξωσωματικής γονιμοποίησης. [Internet] [Thesis]. Harokopio University; Χαροκόπειο Πανεπιστήμιο; 2020. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10442/hedi/47588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Karagiannis D. Συσχέτιση της μεσογειακής δίαιτας με τη γονιμότητα: μελέτη σε δείγμα υπογόνιμων ζευγαριών που ακολουθούν πρόγραμμα εξωσωματικής γονιμοποίησης. [Thesis]. Harokopio University; Χαροκόπειο Πανεπιστήμιο; 2020. Available from: http://hdl.handle.net/10442/hedi/47588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Amanda Begatti Victorino. Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada.

Degree: 2009, Universidade Federal de São Paulo

URL: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=1724

►

Verificar a relacao entre as concentracoes de substancias reativas ao acido tiobarbiturico, a atividade das enzimas glutationa peroxidase e catalase com a ocorrencia de gravidez,… (more)

▼

Verificar a relacao entre as concentracoes de substancias reativas ao acido tiobarbiturico, a atividade das enzimas glutationa peroxidase e catalase com a ocorrencia de gravidez, com o estimulo hormonal administrado e com a presenca ou nao de um fator de infertilidade feminino. Metodo: Foi realizado um estudo prospectivo caso controle com o liquido folicular de 146 pacientes que foram submetidas ao programa de Reproducao Assistida da UNIFESP. Como criterios de inclusao foram considerados apenas as pacientes que realizaram o protocolo de fertilizacao in vitro (FIV) atraves de injecao intracitoplasmatica de espermatozoide (ICSI). Os grupos experimentais foram subdivididos de acordo com a presenca ou nao de gestacao, com hormonio administrado durante o estimulo (FSH ou FSH e hMG) e com a presenca ou nao de pelo menos um fator de infertilidade feminina. As aliquotas dos liquidos foliculares foram coletadas apos a retirada dos oocitos e analisadas por espectrofotometro para mensurar as concentracoes de substancias reativas ao acido tiobarbiturico (TBARS) . um marcador de peroxidacao lipidica, e das enzimas catalase e glutationa peroxidase presentes em cada amostra. Um modelo de regressao logistica foi construido utilizando numero de embrioes de alta qualidade no terceiro dia de cultivo transferidos, presenca de pelo menos um fator de infertilidade feminino e a atividade da glutationa peroxidase, maximizando a preditibilidade da ocorrencia de gravidez. Resultados: No grupo de mulheres nao gravidas foi observada uma maior atividade da enzima glutationa peroxidase (p=0,04). Nos outros grupos nao foi observada diferenca estatistica entre nenhum dos parametros avaliados. Na regressao logistica, o melhor modelo preditivo para gravidez incluiu os valores de fator de infertilidade, classificacao embrionaria no terceiro dia de cultivo e a atividade de glutationa peroxidase (73,68%, p=0.00001). O valor de odds-ratio para a atividade de glutationa peroxidase foi 0,98, indicando que valores crescentes de atividade glutationa levaram a diminuicao da chance de ocorrencia de gravidez. Conclusao: Nas condicoes deste estudo, (i) mulheres com À-hCG negativo possuem uma maior atividade da glutationa peroxidase, (ii) diferentes estimulos ovarianos nao provocam alteracao nas concentracoes de TBARS e na atividade das enzimas antioxidantes e (iii) a presenca de pelo menos um fator de infertilidade feminino nao altera a concentracao de TBARS e a atividade da enzimas antioxidantes.

Objective: to verify the relation between thiobarbituric acid reactive substances concentrations, glutathione peroxidase and catalase activities, and occurrence of pregnancy, hormonal stimulation protocol utilized, and presence or not of a female infertility factor. Method: a prospective case-control study was carried with follicular fluid from 146 patients submitted to the Assisted Reproduction program at the Sao Paulo Federal University. Only patients submitted to in vitro fertilization (FIV) through intracytoplasmic sperm injection (ICSI) were included. The…

Advisors/Committee Members: Ricardo Pimenta Bertolla, Edson Guimarães Lo Turco, Aarão Mendes Pinto Neto, Renato Fraietta, Christina Ramires Ferreira, Felipe Perecin.

Subjects/Keywords: Líquido folicular; Antioxidantes; Radicais Livres; Fertilização in vitro; CIRURGIA UROLOGICA; Oxidative Stress; Follicular Fluid; Antioxidants; Free Radical; in vitro fertilization; Estresse oxidativo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Victorino, A. B. (2009). Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada. (Thesis). Universidade Federal de São Paulo. Retrieved from http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=1724

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Victorino, Amanda Begatti. “Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada.” 2009. Thesis, Universidade Federal de São Paulo. Accessed March 06, 2021. http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=1724.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Victorino, Amanda Begatti. “Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada.” 2009. Web. 06 Mar 2021.

Vancouver:

Victorino AB. Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada. [Internet] [Thesis]. Universidade Federal de São Paulo; 2009. [cited 2021 Mar 06]. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=1724.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Victorino AB. Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada. [Thesis]. Universidade Federal de São Paulo; 2009. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=1724

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Autònoma de Barcelona

21. Roura Llerda, Montserrat. Fatty acids goat follicular fluid: effect on oocyte competence.

Degree: Departament de Ciència Animal i dels Aliments, 2016, Universitat Autònoma de Barcelona

URL: http://hdl.handle.net/10803/399336

► Fatty acids (AAGG) are an important source of energy for oocyte growth phase. Therefore, the study of follicular fluid (FF) where they mature, is of… (more)

▼ Fatty acids (AAGG) are an important source of energy for oocyte growth phase. Therefore, the study of follicular fluid (FF) where they mature, is of special interest. In previous studies, Romaguera et al. (2011) concluded that oocytes from large follicles (≥ 3mm) were more competent than the small follicles. Moreover, Catalá et al. (2015) showed that there was a differential production of embryos between seasons, being significantly lower in autumn than in winter. Our hypothesis was that the FA profile of FF from prepubertal and adult goats, as well as from different follicle sizes and different seasons, could give us information of the oocyte competence. Our objective was to determine whether this profile could be used as a molecular marker for the quality oocytes. In the first experiment, we analysed the profile of FA in FF. Among others, we found differences in total AAGG acids (PUFA), and n6: n3 ratio according to age, size of the follicle and seasons. In the second experiment, we evaluate the competence of oocytes from prepubertal females adding different ratios of linoleic acid (LA: n6 PUFA) and α-linolenic acid (ALA: n3 PUFA) in IVM. We observed ratio LA: ALA 200: 50 µM had a detrimental effect on embryo development of oocytes when compared with control groups and other treatments (100: 50 and 50:50 µM) when oocytes were in vitro fertilized (IVF) (≈ 2.63% vs 13%) but not when they were activated partenogenèticament. According to the results of the experiment 2, the aim of the experiment 3 was to study the effect of LA:ALA ratios on prepubertal goat oocyte quality by assessing mitochondrial distribution and activity, ATP concentration and relative gene expression. Assessing mitochondrial activity, active mitochondria distribution and ATP concentration in the oocyte, we found that there was a change in this parameters when they were analysed on immature oocytes (collection point) compared to IVM oocytes (after 24 h of maturation). Moreover, the addition of 200:50 µM at IVM modified the mitochondrial activity of these oocytes, being higher compared with the other treatment groups, but no changes were observed in the active mitochondria distribution or ATP concentration. Concerning mRNA relative expression, we analysed 9 genes that are shown to be altered if the cell is under stress, and which development could be compromised: ATF4, DNMT1, GAPDH, GCLC, GPX1/GSH-Px, HSPA5/GPR78, RPL19, SLC2A1/GLUT1, SOD1/CuZnSOD. Among these genes, GPX1, RPL19 and SOD1 showed significant differences when comparing immature and IVM oocytes, but not among groups of treatment. In conclusion, we found that FA profile of goat FF is similar to the follicular fluid found in cows, sheep and woman. The main differences that we found in goats were mainly due to the age of the female. However, we found a direct relationship between n6:n3 PUFA composition in follicular fluid regarding follicular size and season of the year, with previous results in our lab suggesting that this ratio could be a biomarker of oocyte competence. Moreover, we… Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Paramio Nieto, Maria Teresa (director), Gracia Catalá, María (director), true (authorsendemail).

Subjects/Keywords: Àcids grassos; Ácidos grasos; Fatty acids; Líquid fol·licular; Liquido folicular; Follicular fluid; Competència oocitària; Competencia oocitária; Oocyte competence; Ciències de la Salut; 573

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roura Llerda, M. (2016). Fatty acids goat follicular fluid: effect on oocyte competence. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/399336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roura Llerda, Montserrat. “Fatty acids goat follicular fluid: effect on oocyte competence.” 2016. Thesis, Universitat Autònoma de Barcelona. Accessed March 06, 2021. http://hdl.handle.net/10803/399336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roura Llerda, Montserrat. “Fatty acids goat follicular fluid: effect on oocyte competence.” 2016. Web. 06 Mar 2021.

Vancouver:

Roura Llerda M. Fatty acids goat follicular fluid: effect on oocyte competence. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2016. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10803/399336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roura Llerda M. Fatty acids goat follicular fluid: effect on oocyte competence. [Thesis]. Universitat Autònoma de Barcelona; 2016. Available from: http://hdl.handle.net/10803/399336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Johnson, Paula Ingrid. Human Exposure to Brominated Flame Retardants and Reproductive Health.

Degree: PhD, Environmental Health Sciences, 2012, University of Michigan

URL: http://hdl.handle.net/2027.42/91595

► Brominated flame retardants (BFRs) such as polybrominated diphenyl ethers (PBDEs) and other compounds are used in the manufacture of a variety of materials and consumer… (more)

▼ Brominated flame retardants (BFRs) such as polybrominated diphenyl ethers (PBDEs) and other compounds are used in the manufacture of a variety of materials and consumer products to meet fire safety standards. BFRs persist in the environment and have been detected in wildlife, humans and indoor dust and air. Some BFRs have demonstrated adverse endocrine and reproductive effects, but human studies are limited. We investigated markers of exposure to BFRs using serum, ovarian follicular fluid and house dust collected from men and women attending infertility clinics. House dust concentrations of the major pentaBDE commercial formulation congeners (BDE 47, 99 and 100) were highly correlated (r=0.65-0.89) to serum concentrations of the same congeners, suggesting that dust is a major exposure source of these PBDEs. Serum concentrations of these congeners were also strongly correlated (r=0.85) between males and females, indicating that adults living in the same household have similar exposures. PBDE congeners in dust were grouped into penta-, octa- and deca-BDEs, resembling commercial mixtures, and alterations in hormone levels in men were modeled in relation to PBDE exposure. Significant positive associations (p<0.05) were found between dust concentrations of pentaBDEs and serum levels of thyroid hormones T4 and T3, estradiol, sex hormone binding globulin (SHBG) and prolactin, along with an inverse association with follicle stimulating hormone. Positive associations between octaBDE concentrations and serum T4, thyroid stimulating hormone, luteinizing hormone and testosterone, and an inverse association between decaBDE concentrations and testosterone, were also found. Relationships between alternate BFRs and hormone levels were examined. Hexabromocyclododecane was associated with decreased SHBG and increased free androgen index. The association between serum and follicular fluid concentrations of PBDEs and failed embryo implantation was investigated. Women with detectable levels of BDE 153 in follicular fluid had elevated odds (adjusted OR=10.0, 95%CI: 1.9-52) of failed embryo implantation following in vitro fertilization (IVF), compared with women who had non-detectable concentrations. There was only a moderate correlation (T<0.4) between serum and follicular fluid concentrations of PBDEs; therefore follicular fluid PBDEs, which may be a more biologically relevant measure of exposure when studying IVF endpoints, may not be well-estimated by serum concentrations of PBDEs. Advisors/Committee Members: Meeker, John D. (committee member), Hu, Howard (committee member), Loch-Caruso, Rita (committee member), Mukherjee, Bhramar (committee member).

Subjects/Keywords: Flame Retardants; PBDEs; Human Exposure; House Dust; Follicular Fluid Contaminants; Public Health; Health Sciences

…in serum (n=63) and follicular fluid (n=64) �� ….….97… …and follicular fluid �� …...98 Table 4.4. Distribution of follicular fluid:serum… …x28;above median, or, if <50% detected, detectable) levels in serum or follicular fluid… …limited. We investigated markers of exposure to BFRs using serum, ovarian follicular fluid and… …between serum and follicular fluid concentrations of PBDEs and failed embryo implantation was…

7 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Johnson, P. I. (2012). Human Exposure to Brominated Flame Retardants and Reproductive Health. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/91595

Chicago Manual of Style (16^th Edition):

Johnson, Paula Ingrid. “Human Exposure to Brominated Flame Retardants and Reproductive Health.” 2012. Doctoral Dissertation, University of Michigan. Accessed March 06, 2021. http://hdl.handle.net/2027.42/91595.

MLA Handbook (7^th Edition):

Johnson, Paula Ingrid. “Human Exposure to Brominated Flame Retardants and Reproductive Health.” 2012. Web. 06 Mar 2021.

Vancouver:

Johnson PI. Human Exposure to Brominated Flame Retardants and Reproductive Health. [Internet] [Doctoral dissertation]. University of Michigan; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2027.42/91595.

Council of Science Editors:

Johnson PI. Human Exposure to Brominated Flame Retardants and Reproductive Health. [Doctoral Dissertation]. University of Michigan; 2012. Available from: http://hdl.handle.net/2027.42/91595

23. Gervais, Alexandre. Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques.

Degree: M. Sc., Physiologie, 2014, Université de Sherbrooke

URL: http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_5897.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/5897/6/Gervais_Alexandre_MSc_2014.pdf

► Résumé : Le syndrome des ovaires polykystiques (SOPK) affecte 6-10% des femmes en âge de procréer et est la principale cause d’infertilité anovulatoire. Cette condition… (more)

▼ Résumé : Le syndrome des ovaires polykystiques (SOPK) affecte 6-10% des femmes en âge de procréer et est la principale cause d’infertilité anovulatoire. Cette condition est principalement caractérisée par une hyperandrogénie provenant des ovaires et des glandes surrénales. De plus en plus d’études, à la fois in vivo et in vitro, indiquent qu’une surexposition ovarienne en gras, de même que les mécanismes lipotoxiques subséquents pourraient causer l’hyperandrogénie chez ces femmes. Ainsi, cette étude vise à 1) évaluer l’implication des gras et de l’inflammation folliculaire dans la production des androgènes ovariens par une étude de corrélation; et 2) comparer les niveaux folliculaires de gras, métabolites lipidiques et marqueurs inflammatoires entre les femmes SOPK et non-SOPK. Cette étude transversale a recruté 80 femmes en cours de fécondation in vitro. Treize étaient SOPK, 58 étaient non-SOPK et 9 avaient un diagnostic incertain. Les participantes au projet avaient un âge moyen de 33 ± 4 ans. Tout groupe confondus, les niveaux folliculaires de testostérone corrélaient avec les gras (r=0,381; P=0,001; indépendamment de l’IL-6), les acylcarnitines (r≥0,255; tout P=0,008; non indépendamment des gras) et l’IL-6 (r=0,300; P=0,009; indépendamment des gras). De plus, les niveaux folliculaires de gras corrélaient avec les acylcarnitines (r≥0,594; tout P<0,001). Lorsque comparées aux femmes non-SOPK, les femmes SOPK étaient plus obèses (BMI: 31,2 vs. 25,1 kg/m2; P=0,009) et avaient des niveaux folliculaires plus élevés de testostérone (12,9 vs. 0,29 nM; P=0,001), de gras (P=0,001), d’acylcarnitines (tout P=0,001) et du marqueur inflammatoire IL-6 (13,0 vs. 8.6 pg/mL; P=0,018), le tout indépendamment de l’IMC. Les résultats suggèrent qu’une exposition intra-ovarienne en gras pourrait contribuer à une augmentation de la production des androgènes. Les mécanismes pourraient inclure une β-oxydation insuffisante, illustré par des acylcarnitines élevés, menant à l’accumulation de métabolites lipidiques toxiques, et une inflammation ovarienne, possiblement suite à une infiltration de macrophages. Ces deux mécanismes semblent être indépendants. De plus, l’environnement ovarien des femmes SOPK est caractérisé par une élévation des gras, de métabolites lipidiques et de marqueurs inflammatoires. Il peut donc être proposer que l’hyperandrogénie caractéristique du SOPK soit causée en partie par la lipotoxicité. // Abstract : Polycystic ovary syndrome (PCOS) affects 6-10% of the women of childbearing age and is the main cause of anovulatory infertility. It is mainly characterised by hyperandrogenism that can originate from the adrenal gland and the ovaries. A growing number of evidences, either in vivo or in vitro, points toward an overexposition to fat and lipotoxic mechanisms (lipid induced cellular toxicity) as the cause of PCOS hyperandrogenesis. Therefore, we aimed to: 1) evaluate the implication of follicular fluid (FF) levels of fat and inflammation markers in the ovarian production of androgens through a correlation analysis;… Advisors/Committee Members: Baillargeon, Jean-Patrice.

Subjects/Keywords: Lipotoxicité; Androgénèse; Liquide folliculaire; Syndrome des ovaires polykystiques; Lipotoxicity; Androgenesis; Follicular fluid; Polycystic ovary syndrome

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APA (6^th Edition):

Gervais, A. (2014). Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques. (Masters Thesis). Université de Sherbrooke. Retrieved from http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_5897.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/5897/6/Gervais_Alexandre_MSc_2014.pdf

Chicago Manual of Style (16^th Edition):

Gervais, Alexandre. “Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques.” 2014. Masters Thesis, Université de Sherbrooke. Accessed March 06, 2021. http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_5897.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/5897/6/Gervais_Alexandre_MSc_2014.pdf.

MLA Handbook (7^th Edition):

Gervais, Alexandre. “Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques.” 2014. Web. 06 Mar 2021.

Vancouver:

Gervais A. Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques. [Internet] [Masters thesis]. Université de Sherbrooke; 2014. [cited 2021 Mar 06]. Available from: http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_5897.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/5897/6/Gervais_Alexandre_MSc_2014.pdf.

Council of Science Editors:

Gervais A. Implication de la lipotoxicité ovarienne dans le syndrome des ovaires polykystiques. [Masters Thesis]. Université de Sherbrooke; 2014. Available from: http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_5897.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/5897/6/Gervais_Alexandre_MSc_2014.pdf

University of Florida

24. DESVOUSGES, ANDRIA L. ( Author, Primary ). Tissue Remodeling and Steroidogenesis in the Preovulatory Follicle of Cycling Pony Mares.

Degree: University of Florida

URL: https://ufdc.ufl.edu/UFE0008962

Subjects/Keywords: Enzymes; Error rates; Follicular fluid; Gels; Gonadotropins; Horses; Least squares; Mathematical dependent variables; Messenger RNA; Ovulation

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APA (6^th Edition):

DESVOUSGES, ANDRIA L. ( Author, P. ). (n.d.). Tissue Remodeling and Steroidogenesis in the Preovulatory Follicle of Cycling Pony Mares. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0008962

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

DESVOUSGES, ANDRIA L. ( Author, Primary ). “Tissue Remodeling and Steroidogenesis in the Preovulatory Follicle of Cycling Pony Mares.” Thesis, University of Florida. Accessed March 06, 2021. https://ufdc.ufl.edu/UFE0008962.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

DESVOUSGES, ANDRIA L. ( Author, Primary ). “Tissue Remodeling and Steroidogenesis in the Preovulatory Follicle of Cycling Pony Mares.” Web. 06 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

DESVOUSGES, ANDRIA L. ( Author P). Tissue Remodeling and Steroidogenesis in the Preovulatory Follicle of Cycling Pony Mares. [Internet] [Thesis]. University of Florida; [cited 2021 Mar 06]. Available from: https://ufdc.ufl.edu/UFE0008962.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

DESVOUSGES, ANDRIA L. ( Author P). Tissue Remodeling and Steroidogenesis in the Preovulatory Follicle of Cycling Pony Mares. [Thesis]. University of Florida; Available from: https://ufdc.ufl.edu/UFE0008962

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Florida

25. Luzbel De la Sota, Rodolfo, 1959-. Endocrine and molecular changes associated with ovarian follicular dominance in cattle.

Degree: PhD, Animal Science, 1995, University of Florida

URL: https://ufdc.ufl.edu/AA00041136

Subjects/Keywords: Cattle; Enzymes; Estrus cycle; Follicular fluid; Gene expression; Granulosa cells; Messenger RNA; Ovaries; Rats; RNA; Cattle – Reproduction

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APA (6^th Edition):

Luzbel De la Sota, Rodolfo, 1. (1995). Endocrine and molecular changes associated with ovarian follicular dominance in cattle. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00041136

Chicago Manual of Style (16^th Edition):

Luzbel De la Sota, Rodolfo, 1959-. “Endocrine and molecular changes associated with ovarian follicular dominance in cattle.” 1995. Doctoral Dissertation, University of Florida. Accessed March 06, 2021. https://ufdc.ufl.edu/AA00041136.

MLA Handbook (7^th Edition):

Luzbel De la Sota, Rodolfo, 1959-. “Endocrine and molecular changes associated with ovarian follicular dominance in cattle.” 1995. Web. 06 Mar 2021.

Vancouver:

Luzbel De la Sota, Rodolfo 1. Endocrine and molecular changes associated with ovarian follicular dominance in cattle. [Internet] [Doctoral dissertation]. University of Florida; 1995. [cited 2021 Mar 06]. Available from: https://ufdc.ufl.edu/AA00041136.

Council of Science Editors:

Luzbel De la Sota, Rodolfo 1. Endocrine and molecular changes associated with ovarian follicular dominance in cattle. [Doctoral Dissertation]. University of Florida; 1995. Available from: https://ufdc.ufl.edu/AA00041136

University of Florida

26. Diaz Zambrano, Thais del Valle, 1957-. Mechanisms that control follicular dominance in cattle.

Degree: PhD, Animal Science, 1998, University of Florida

URL: https://ufdc.ufl.edu/AA00029979

Subjects/Keywords: Cattle; Cultured cells; Dosage; Estrus cycle; Follicular fluid; Granulosa cells; Heifers; Ovaries; Secretion; Theca cells; Cattle – Reproduction – Endocrine aspects; Ovulation – Induction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Diaz Zambrano, Thais del Valle, 1. (1998). Mechanisms that control follicular dominance in cattle. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00029979

Chicago Manual of Style (16^th Edition):

Diaz Zambrano, Thais del Valle, 1957-. “Mechanisms that control follicular dominance in cattle.” 1998. Doctoral Dissertation, University of Florida. Accessed March 06, 2021. https://ufdc.ufl.edu/AA00029979.

MLA Handbook (7^th Edition):

Diaz Zambrano, Thais del Valle, 1957-. “Mechanisms that control follicular dominance in cattle.” 1998. Web. 06 Mar 2021.

Vancouver:

Diaz Zambrano, Thais del Valle 1. Mechanisms that control follicular dominance in cattle. [Internet] [Doctoral dissertation]. University of Florida; 1998. [cited 2021 Mar 06]. Available from: https://ufdc.ufl.edu/AA00029979.

Council of Science Editors:

Diaz Zambrano, Thais del Valle 1. Mechanisms that control follicular dominance in cattle. [Doctoral Dissertation]. University of Florida; 1998. Available from: https://ufdc.ufl.edu/AA00029979

27. Ανυφαντής, Γεώργιος-Σπυρίδων. Επίπεδα λεπτίνης στον ορό και στο ωοθυλακικό υγρό γυναικών που υποβάλλονται σε πρόγραμμα εξωσωματικής γονιμοποίησης - εμβρυομεταφοράς ( IVF-ET)ς: συσχέτιση με όλες τις παραμέτρους της υποβοηθούμενης αναπαραγωγής.

Degree: 2004, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/15474

Subjects/Keywords: Λεπτίνη; Ωοθυλακικό υγρό; Ωάρια; Έμβρυα; Κύηση; Υποβοηθούμενη αναπαραγωγή; Leptin; Follicular fluid; Oocytes; Embryos; Pregnancy; Assisted reproduction

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APA (6^th Edition):

Ανυφαντής, . �. (2004). Επίπεδα λεπτίνης στον ορό και στο ωοθυλακικό υγρό γυναικών που υποβάλλονται σε πρόγραμμα εξωσωματικής γονιμοποίησης - εμβρυομεταφοράς ( IVF-ET)ς: συσχέτιση με όλες τις παραμέτρους της υποβοηθούμενης αναπαραγωγής. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/15474

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ανυφαντής, Γεώργιος-Σπυρίδων. “Επίπεδα λεπτίνης στον ορό και στο ωοθυλακικό υγρό γυναικών που υποβάλλονται σε πρόγραμμα εξωσωματικής γονιμοποίησης - εμβρυομεταφοράς ( IVF-ET)ς: συσχέτιση με όλες τις παραμέτρους της υποβοηθούμενης αναπαραγωγής.” 2004. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 06, 2021. http://hdl.handle.net/10442/hedi/15474.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ανυφαντής, Γεώργιος-Σπυρίδων. “Επίπεδα λεπτίνης στον ορό και στο ωοθυλακικό υγρό γυναικών που υποβάλλονται σε πρόγραμμα εξωσωματικής γονιμοποίησης - εμβρυομεταφοράς ( IVF-ET)ς: συσχέτιση με όλες τις παραμέτρους της υποβοηθούμενης αναπαραγωγής.” 2004. Web. 06 Mar 2021.

Vancouver:

Ανυφαντής �. Επίπεδα λεπτίνης στον ορό και στο ωοθυλακικό υγρό γυναικών που υποβάλλονται σε πρόγραμμα εξωσωματικής γονιμοποίησης - εμβρυομεταφοράς ( IVF-ET)ς: συσχέτιση με όλες τις παραμέτρους της υποβοηθούμενης αναπαραγωγής. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2004. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10442/hedi/15474.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ανυφαντής �. Επίπεδα λεπτίνης στον ορό και στο ωοθυλακικό υγρό γυναικών που υποβάλλονται σε πρόγραμμα εξωσωματικής γονιμοποίησης - εμβρυομεταφοράς ( IVF-ET)ς: συσχέτιση με όλες τις παραμέτρους της υποβοηθούμενης αναπαραγωγής. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2004. Available from: http://hdl.handle.net/10442/hedi/15474

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

28. Pelzer, Elise Sarah. Microbial colonisation of human follicular fluid and adverse in vitro fertilisation outcomes.

Degree: 2011, Queensland University of Technology

URL: https://eprints.qut.edu.au/49122/

► This study, investigating 263 women undergoing trans-vaginal oocyte retrieval for in vitro fertilisation (IVF) found that microorganisms colonising follicular fluid contributed to adverse IVF (pre-implantation)… (more)

Subjects/Keywords: assisted reproductive technology (ART), follicular fluid, upper genital tract, lower genital tract, steroid hormones, oestradiol, progesterone, biofilm, Lactobacillus species, trans-vaginal oocyte retrieval, semen, washed semen, cytokines, microorganisms; endometriosis, polycystic ovary syndrome, genital tract infection, genital tract colonisation or contamination, male factor infertility, fertilisation, embryo transfer, pregnancy, in vitro fertilisation (IVF)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pelzer, E. S. (2011). Microbial colonisation of human follicular fluid and adverse in vitro fertilisation outcomes. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/49122/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pelzer, Elise Sarah. “Microbial colonisation of human follicular fluid and adverse in vitro fertilisation outcomes.” 2011. Thesis, Queensland University of Technology. Accessed March 06, 2021. https://eprints.qut.edu.au/49122/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pelzer, Elise Sarah. “Microbial colonisation of human follicular fluid and adverse in vitro fertilisation outcomes.” 2011. Web. 06 Mar 2021.

Vancouver:

Pelzer ES. Microbial colonisation of human follicular fluid and adverse in vitro fertilisation outcomes. [Internet] [Thesis]. Queensland University of Technology; 2011. [cited 2021 Mar 06]. Available from: https://eprints.qut.edu.au/49122/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pelzer ES. Microbial colonisation of human follicular fluid and adverse in vitro fertilisation outcomes. [Thesis]. Queensland University of Technology; 2011. Available from: https://eprints.qut.edu.au/49122/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. ΠΑΠΠΑ, ΑΓΛΑΙΑ. ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝ ΓΟΝΑΔΟΤΡΟΦΙΝΩΝ (GONADOTROPHINSURGE ATTENUATING FACTOR - GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ.

Degree: 1998, Πανεπιστήμιο Ιωαννίνων; University of Ioannina

URL: http://hdl.handle.net/10442/hedi/10966

►

ΣΚΟΠΟΣ ΤΗΣ ΔΙΑΤΡΙΒΗΣ ΗΤΑΝ Η ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝΓΟΝΟΔΟΤΡΟΦΙΝΩΝ (GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ. ΓΙΑ ΤΗΝ ΠΑΡΑΚΟΛΟΥΘΗΣΗ ΤΗΣ ΔΡΑΣΤΙΚΟΤΗΤΑΣ ΤΟΥ GNSAF ΧΡΗΣΙΜΟΠΟΙΗΘΗΚΕ… (more)

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ΣΚΟΠΟΣ ΤΗΣ ΔΙΑΤΡΙΒΗΣ ΗΤΑΝ Η ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝΓΟΝΟΔΟΤΡΟΦΙΝΩΝ (GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ. ΓΙΑ ΤΗΝ ΠΑΡΑΚΟΛΟΥΘΗΣΗ ΤΗΣ ΔΡΑΣΤΙΚΟΤΗΤΑΣ ΤΟΥ GNSAF ΧΡΗΣΙΜΟΠΟΙΗΘΗΚΕ ΤΟ IN VITRO ΣΥΣΤΗΜΑ ΤΩΝ ΠΡΩΤΟΓΕΝΩΝ ΚΑΛΛΙΕΡΓΕΙΩΝ ΚΥΤΤΑΡΩΝ ΥΠΟΦΥΣΗΣ ΕΠΙΜΥΩΝ. Η ΔΡΑΣΤΙΚΟΤΗΤΑ GNSAF ΕΚΦΡΑΖΕΤΑΙ ΩΣ ΣΗΜΑΝΤΙΚΗ ΕΛΑΤΤΩΣΗ ΤΗΣ ΕΚΚΡΙΣΗΣ ΤΗΣ LH ΤΩΝ ΥΠΟΦΥΣΙΑΚΩΝ ΚΥΤΤΑΡΩΝ ΜΕΤΑ ΑΠΟ ΔΙΕΓΕΡΣΗ ΜΕ GNRH. ΔΡΑΣΤΙΚΟΤΗΤΑ GNSAF ΑΠΟΜΟΝΩΘΗΚΕ ΑΠΟ 250 ML ΩΟΘΥΛΑΚΙΚΟΥ ΥΓΡΟΥ ΓΥΝΑΙΚΩΝ. ΑΠΟ ΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΑΦΑΙΡΕΘΗΚΑΝ ΟΙ ΣΤΕΡΟΕΙΔΕΙΣ ΟΡΜΟΝΕΣ ΚΑΙ ΑΚΟΛΟΥΘΗΣΕ ΘΕΡΜΑΝΣΗ (80C, 5MIN), ΧΡΩΜΑΤΟΓΡΑΦΙΑ HEPARIN-SEPHAROSE ΚΑΙ CON-A, ΥΓΡΗ ΧΡΩΜΑΤΟΓΡΑΦΙΑ ΥΨΗΛΗΣ ΠΙΕΣΗΣ ΣΕ ΣΤΗΛΗ ΑΝΑΣΤΡΟΦΟΥ ΦΑΣΕΩΣ RYDAC C4 ΚΑΙ ΗΛΕΚΤΡΟΦΟΡΗΣΗ ΥΠΟ ΜΗ ΜΕΤΟΥΣΙΩΤΙΚΕΣ ΣΥΝΘΗΚΕΣ. ΧΡΗΣΙΜΟΠΟΙΩΝΤΑΣ ΤΑ 6 ΑΥΤΑ ΣΤΑΔΙΑ ΚΑΘΑΡΙΣΜΟΥ Η ΔΡΑΣΤΙΚΟΤΗΤΑ GNSAF ΑΠΟΜΟΝΩΘΗΚΕ ΣΕ ΜΙΑ ΟΜΟΙΟΓΕΝΗ ΠΡΩΤΕ~ΝΙΚΗ ΖΩΝΗ,ΟΠΩΣ ΕΔΕΙΞΕ Η ΑΝΑΛΥΣΗ ΤΩΝ ΠΡΩΤΕ~ΝΩΝ ΜΕΤΑ ΑΠΟ ΗΛΕΚΤΡΟΦΟΡΗΣΗ ΥΠΟ ΜΕΤΟΥΣΙΩΤΙΚΕΣ ΣΥΝΘΗΚΕΣ. ΒΑΣΕΙ ΤΩΝ ΑΠΟΤΕΛΕΣΜΑΤΩΝ ΑΥΤΩΝ Ο GNSAF ΕΙΝΑΙ ΕΝΩΣΗ ΑΝΘΕΚΤΙΚΗ ΣΤΗ ΘΕΡΜΟΚΡΑΣΙΑ ΤΩΝ 80C, ΓΛΥΚΟΠΡΩΤΕ~ΝΗ (Η ΔΡΑΣΤΙΚΚΟΤΗΤΑ ΑΝΙΧΝΕΥΘΗΚΕ ΣΤΑ ΔΕΣΜΕΥΜΕΝΑ ΚΛΑΣΜΑΤΑ ΤΗΣ ΣΤΗΛΗΣ CON-A), ΑΝΘΕΚΤΙΚΗ ΣΤΟΥΣ ΟΡΓΑΝΙΚΟΥΣ ΔΙΑΛΥΤΕΣ ΚΑΙ ΜΕ ΜΟΡΙΑΚΟ ΒΑΡΟΣ 12,5 KDA. ΜΕΡΙΚΗ ΑΝΑΛΥΣΗ

THE PURPOSE OF THE DISSERTATION WAS TO ISOLATE GNSAF BIOACTIVITY (SUPPRESSIONOF THE PITVITARY RESPONSE TO GNRH) FROM HUMAN FOLLICULAR FLUID. IN ORDER TOMEASURE GNSAF BIOACTIVITY RAT PITVITARY CELLS IN SHORT-TERM CULTURE WERE USED. GNSAF ACTIVITY WAS DEFINED AS THE SUPPRESSION OF GNRH-STIMULATED LH SECRETION. GNSAF ACTIVITY WAS PURIFIED FROM 250ML OF TWICE-FOLD STEROID EXTRACTEDHUMAN FOLLICULAR FLUID USING HEAT TREATMENT (80C, 5MIN) HEPARIN-SEPHAROSE AND CON-A CHROMATOGRAPHY, VYDAC C4 REVERSED OHASE HPLC AND ROD GEL NATIVE ELECTROPHORESIS. USING THESE SIX PURIFICATION STEPS, WE HAVE OBTAINED AN APPARENTLY HOMOGENOUS PREPARATION THAT STAINS AS A SINGLE BAND ON SDS -POLYACPYLA- MIDE GEL ELECTROPHORESIS. GNSAF IS A HEAT-RESISTANT MOLECULE AT 80C, A GLYCOPROTEIN -SINCE ITS ACTIVITY WAS RESOLVED IN THE BOUND FRACTIONS OF CON-A SEPHAROSE-, RESISTANT AFTER ACETONITRILE TREATMENT - THE PROTEIN WAS NOT DENATURATED BY THE ORGANIC SOLVENT USED IN RP-HPLC AND WITH AN APPARENT MOLECULAR WEIGHT OF 12,5 KDA. MICROSEQUENCE ANALYSIS BY MASS SPECTROMETRY OF THE GNSAF PROTEIN REVEALED IDENTITY TO THE C-TERMINAL FRAGMENT OF HUMAN SERUM ALBUMIN. THE GLYCOSYLATED SITES OF THE PROTEIN PROBABLY DO NOT AFFECT ITS ACTIVITY. THE MECHANISM BY WHICH GNSAF MAINTAINS PITUITARY IN A STATE OF LOW RESPONSIVENES TO GNRH IS UNCLEAR. PRELIMINARY STUDIES SHOWED THAT GNSAF SUPPRESSES GNRH SELF-PRIMING EFFECT, PROTEIN KINASE C IS NOT INVOLVED IN THE MECHANISM OF ACTION OF GNSAF, WHILE OESTRADIOL MAY PLAY A REGULATORY ROLE.

Subjects/Keywords: Gonadotrophin surge attenuating factor GnSAF; HUMAN FOLLICULAR FLUID; Luteinizing hormone; OVARIAN CYCLE; ΑΝΘΡΩΠΙΝΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ; Παράγοντας άμβλυνσης του κύματος των γονοδοτροφινών; ΩΟΘΥΚΙΚΟΣ ΚΥΚΛΟΣ; Ωχρινοτρόπος ορμόνη

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΠΑΠΠΑ, �. (1998). ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝ ΓΟΝΑΔΟΤΡΟΦΙΝΩΝ (GONADOTROPHINSURGE ATTENUATING FACTOR - GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ. (Thesis). Πανεπιστήμιο Ιωαννίνων; University of Ioannina. Retrieved from http://hdl.handle.net/10442/hedi/10966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΠΑΠΠΑ, ΑΓΛΑΙΑ. “ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝ ΓΟΝΑΔΟΤΡΟΦΙΝΩΝ (GONADOTROPHINSURGE ATTENUATING FACTOR - GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ.” 1998. Thesis, Πανεπιστήμιο Ιωαννίνων; University of Ioannina. Accessed March 06, 2021. http://hdl.handle.net/10442/hedi/10966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΠΑΠΠΑ, ΑΓΛΑΙΑ. “ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝ ΓΟΝΑΔΟΤΡΟΦΙΝΩΝ (GONADOTROPHINSURGE ATTENUATING FACTOR - GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ.” 1998. Web. 06 Mar 2021.

Vancouver:

ΠΑΠΠΑ �. ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝ ΓΟΝΑΔΟΤΡΟΦΙΝΩΝ (GONADOTROPHINSURGE ATTENUATING FACTOR - GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ. [Internet] [Thesis]. Πανεπιστήμιο Ιωαννίνων; University of Ioannina; 1998. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10442/hedi/10966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΠΑΠΠΑ �. ΑΠΟΜΟΝΩΣΗ ΤΟΥ ΠΑΡΑΓΟΝΤΑ ΑΜΒΛΥΝΣΗΣ ΤΟΥ ΚΥΜΑΤΟΣ ΤΩΝ ΓΟΝΑΔΟΤΡΟΦΙΝΩΝ (GONADOTROPHINSURGE ATTENUATING FACTOR - GNSAF) ΑΠΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΓΥΝΑΙΚΩΝ. [Thesis]. Πανεπιστήμιο Ιωαννίνων; University of Ioannina; 1998. Available from: http://hdl.handle.net/10442/hedi/10966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. ΦΩΤΟΠΟΥΛΟΣ, ΣΤΑΥΡΟΣ. ΕΠΙΠΕΔΑ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΣΕ ΑΥΤΟΜΑΤΗ ΚΑΙ ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ.

Degree: 1989, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens

URL: http://hdl.handle.net/10442/hedi/5094

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Η ΜΕΛΕΤΗ ΜΑΣ ΠΕΡΙΛΑΜΒΑΝΕΙ 42 ΓΥΝΑΙΚΕΣ ΣΤΙΣ ΟΠΟΙΕΣ ΕΓΙΝΕ ΠΡΟΣΠΑΘΕΙΑ ΕΞΩΣΩΜΑΤΙΚΗΣΓΟΝΙΜΟΠΟΙΗΣΗΣ ΜΕ ΤΗ ΧΟΡΗΓΗΣΗ ΑΝΘΡΩΠΙΝΩΝ ΓΟΝΑΔΟΤΡΟΠΙΝΩΝ ΚΑΙ ΑΝΑΛΟΓΩΝ ΕΚΛΥΤΙΚΩΝΠΑΡΑΓΟΝΤΩΝ ΤΟΥ ΥΠΟΘΑΛΑΜΟΥ (GNRH-A). ΣΑΝ ΜΑΡΤΥΡΕΣ ΧΡΗΣΙΜΟΠΟΙΗΘΗΚΑΝ… (more)

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Η ΜΕΛΕΤΗ ΜΑΣ ΠΕΡΙΛΑΜΒΑΝΕΙ 42 ΓΥΝΑΙΚΕΣ ΣΤΙΣ ΟΠΟΙΕΣ ΕΓΙΝΕ ΠΡΟΣΠΑΘΕΙΑ ΕΞΩΣΩΜΑΤΙΚΗΣΓΟΝΙΜΟΠΟΙΗΣΗΣ ΜΕ ΤΗ ΧΟΡΗΓΗΣΗ ΑΝΘΡΩΠΙΝΩΝ ΓΟΝΑΔΟΤΡΟΠΙΝΩΝ ΚΑΙ ΑΝΑΛΟΓΩΝ ΕΚΛΥΤΙΚΩΝΠΑΡΑΓΟΝΤΩΝ ΤΟΥ ΥΠΟΘΑΛΑΜΟΥ (GNRH-A). ΣΑΝ ΜΑΡΤΥΡΕΣ ΧΡΗΣΙΜΟΠΟΙΗΘΗΚΑΝ 23 ΓΥΝΑΙΚΕΣΜΕ ΦΥΣΙΟΛΟΓΙΚΟ ΓΕΝΝΗΤΙΚΟ ΚΥΚΛΟ. ΑΠΟ ΤΗΝ ΜΕΛΕΤΗ ΚΑΙ ΤΗΝ ΣΤΑΤΙΣΤΙΚΗ ΑΝΑΛΥΣΗ ΤΩΝΤΙΜΩΝ ΤΩΝ ΟΡΜΟΝΩΝ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟΝ ΟΡΟ ΤΟΥ ΑΙΜΑΤΟΣ ΚΑΙ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΠΡΟΕΚΥΨΑΝ ΤΑ ΕΞΗΣ ΣΥΜΠΕΡΑΣΜΑΤΑ: Α) ΤΑ ΕΠΙΠΕΔΑ ΤΗΣΟΙΣΤΡΑΔΙΟΛΗΣ ΚΑΙ ΤΗΣ ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΣΤΟΝ ΟΡΟ ΤΩΝ ΔΥΟ ΟΜΑΔΩΝ ΔΙΕΦΕΡΑΝ ΣΤΑΤΙΣΤΙΚΑ ΣΗΜΑΝΤΙΚΑ, Ρ < = 5,8Χ10-11 ΚΑΙ Ρ < = 2,2Χ10-8. Β) ΟΙ ΤΙΜΕΣ ΚΟΡΤΙΖΟΛΗΣ ΤΟΣΟ ΣΤΟΝ ΟΡΟ ΟΣΟ ΚΑΙ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΔΕΝ ΠΑΡΟΥΣΙΑΖΟΥΝ ΣΤΑΤΙΣΤΙΚΑ ΣΗΜΑΝΤΙΚΗ ΔΙΑΦΟΡΑ.Γ) ΤΑ ΠΟΣΟΣΤΑ ΓΟΝΙΜΟΠΟΙΗΣΗΣ ΤΩΝ ΩΟΚΥΤΤΑΡΩΝ ΔΕΝ ΔΙΕΦΕΡΑΝ ΣΤΑΤΙΣΤΙΚΑ ΣΗΜΑΝΤΙΚΑ ΜΕΤΑΞΥ ΤΩΝ ΔΥΟ ΟΜΑΔΩΝ, ΑΛΛΑ ΤΑ ΠΟΣΟΣΤΑ ΚΥΗΣΕΩΝ ΓΙΑ ΤΙΣ ΓΥΝΑΙΚΕΣ ΜΕ ΚΥΚΛΟΥΣ ΧΩΡΙΣ ΔΙΕΓΕΡΣΗ ΗΣΑΝ 0%, ΕΝΩ ΗΤΑΝ ΥΨΗΛΑ ΓΙΑ ΤΙΣ ΓΥΝΑΙΚΕΣ ΜΕ ΤΟΥΣ ΔΙΕΓΕΡΜΕΝΟΥΣ ΚΥΚΛΟΥΣ 28,5%.

OUR STUDY CONSISTED OF 42 WOMEN WHO RECEIVED GONADOTROPINS AND GNRH-A FOR INDUCTION OF OVULATION FOR IVF. THE CONTROL GROUP INCLUDED 23 NORMAL CYCLING WOMEN.AFTER EVALUATING OUR RESULTS THE FOLLOWING CONCLUSIONS CAN BE DRAWN: 1) SERUMESTRADIOL AND PROGESERONE SHOWED A STATISTICALLY HIGHLY SIGNIFICANT DIFFERENCE BETWEEN THE STIMULATED AND UNSTIMULATED CYCLES, BEING HIGHLY ELEVATED IN THE GROUP OF STIMULATED BECAUSE OF MULTIPLE FOLICULOGENESIS (P < =5,8X10-11, P < = 2,2X10-8). 2) CORTISOL LEVELS IN SERUM AND FOLLICULAR FLUID SHOWED NO STATISTICALLY SIGNIFICANT DIFFERENCE BETWEEN THE TWO GROUPS OF PATIENTS. 3) THE PERCENTAGE OF FERTILIZED OOCYTES SHOWED NO STATISTICALLY SIGNIFICANT DIFFERENCE BETWEEN THE TWO GROUPS, BUT THE PERCENTAGE OF PREGNANCIES WAS 0% FOR THE NORMAL CYCLES, WHILE IT WAS QUITE HIGH 28,5% FOR THE INDUCED CYCLES.

Subjects/Keywords: Cortisol; Estradiol; Follicular fluid; INDUCTION OF OVULATION; IVF (in vitro fertilization); Progesterone; Serum; ΑΥΤΟΜΑΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ; Εξωσωματική γονιμοποίηση; Κορτιζόλη; Οιστραδιόλη; Προγεστερόνη; ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ; Ωοθυλακικό υγρό

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΦΩΤΟΠΟΥΛΟΣ, �. (1989). ΕΠΙΠΕΔΑ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΣΕ ΑΥΤΟΜΑΤΗ ΚΑΙ ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ. (Thesis). Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens. Retrieved from http://hdl.handle.net/10442/hedi/5094

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΦΩΤΟΠΟΥΛΟΣ, ΣΤΑΥΡΟΣ. “ΕΠΙΠΕΔΑ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΣΕ ΑΥΤΟΜΑΤΗ ΚΑΙ ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ.” 1989. Thesis, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens. Accessed March 06, 2021. http://hdl.handle.net/10442/hedi/5094.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΦΩΤΟΠΟΥΛΟΣ, ΣΤΑΥΡΟΣ. “ΕΠΙΠΕΔΑ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΣΕ ΑΥΤΟΜΑΤΗ ΚΑΙ ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ.” 1989. Web. 06 Mar 2021.

Vancouver:

ΦΩΤΟΠΟΥΛΟΣ �. ΕΠΙΠΕΔΑ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΣΕ ΑΥΤΟΜΑΤΗ ΚΑΙ ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ. [Internet] [Thesis]. Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens; 1989. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10442/hedi/5094.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΦΩΤΟΠΟΥΛΟΣ �. ΕΠΙΠΕΔΑ ΟΙΣΤΡΑΔΙΟΛΗΣ, ΠΡΟΓΕΣΤΕΡΟΝΗΣ ΚΑΙ ΚΟΡΤΙΖΟΛΗΣ ΣΤΟ ΩΟΘΥΛΑΚΙΚΟ ΥΓΡΟ ΣΕ ΑΥΤΟΜΑΤΗ ΚΑΙ ΠΡΟΚΛΗΤΗ ΩΟΘΥΛΑΚΙΟΡΡΗΞΙΑ. [Thesis]. Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); National and Kapodistrian University of Athens; 1989. Available from: http://hdl.handle.net/10442/hedi/5094

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations