You searched for subject:(Fluorophores).
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1.
Sadek, Omar.
Exploiting boron-fluorine bonds for fluorination and synthesis of potential bi-modal imaging agents : Exploitation du couple bore-fluor pour la fluoration et la synthèse d'agnet d'imagerie bimodaux potentiels.
Degree: Docteur es, Chimie moléculaire, 2018, Université Toulouse III – Paul Sabatier
URL: http://www.theses.fr/2018TOU30141 
► La liaison Bore-Fluor (B-F) a une longue et riche histoire dans le domaine des transformations chimiques. De plus, cette liaison B-F est utilisée dans une…
(more)
▼ La liaison Bore-Fluor (B-F) a une longue et riche histoire dans le domaine des transformations chimiques. De plus, cette liaison B-F est utilisée dans une grande diversité de domaines tel que la médecine nucléaire avec l'imagerie de Tomographie par Émission de Positrons (TEP) ou l'imagerie de fluorescence. Dans le cadre de cette thèse, nous nous intéressons à l'utilisation de la liaison B-F pour la formation nucléophile de liaison Carbone-Fluor (C-F) et le développement de nouveaux fluorophores ayant des applications potentielles dans les domaines de l'imagerie bimodale TEP et l'imagerie de fluorescence. Les organotrifluoroborates sont connus pour être des outils de synthèse d'importance majeure pour la conception de molécules organiques. Cependant, le groupement trifluoroborate n'est généralement utilisé qu'en tant que groupement partant, c'est à dire par rupture de la liaison C-B. Ainsi, son potentiel en tant que source de fluorure nucléophile (F-) n'est pas étudié. C'est pourquoi, nous nous sommes intéressé à l'aptitude des organotrifluoroborates à servir de source de fluorure. En effet, si historiquement la réaction de Balz-Schiemann, utilise le BF4- comme source de F-, nous avons démontré que les organotrifluoroborates sont capables de réaliser la réaction de fluoro-dédiazotation de sels d'aryldiazonium générés in situ dans des conditions réactionnelles douces. De plus, dans des réactions avec une classe de substrats analogues, des sels dissymétriques de diaryliodonium pourraient être fluorés par des phényltrifluoroborates par décomposition thermique de la paire d'ion. Finalement, l'aptitude du groupement trifluoroborate à promouvoir des réactions de fluoration intramoléculaires a été étudiée par la synthèse de divers motifs contenant ce groupement. Nous avons également étudié de nouveaux complexes fluoroborates (B-Fn) dérivés du motif 2-aryl-benzothiazole comme sondes fluorescentes. Trois complexes ont été isolés, caractérisés, et leurs propriétés photo-physiques rapportées. Un complexe monofluoroborate de 2,4-diaryl-benzothiazole présentant les propriétés photo-physiques les plus prometteuses s'est révélé stable en conditions aqueuses. De plus, l'abstraction de fluorure de ce complexe a été démontrée, fournissant ainsi des résultats préliminaires prometteurs vers la viabilité du radiomarquage par échange isotopiques 19F-18F. Ce complexe monofluoroborate a également montré des propriétés structurales intéressantes par la présence d'un bore stéréogène. Les énantiomères de ce complexe ont été dédoublés par chromatographie chirale à partir du mélange racémique puis caractérisés par dichroïsme circulaire. Enfin, la stabilité configurationnelle du bore a été étudiée.
The B-F bond has an expansive and rich history in chemical transformations and the versatility of the B-F bond has also shown immense utility in fields as far reaching as Positron Emission Tomography (PET) and fluorescence imaging. The application of B-F bonds for nucleophilic Carbon-Fluorine (C-F) bond formation and the development of novel…
Advisors/Committee Members: Gras, Emmanuel (thesis director), Perrin, David (thesis director).
Subjects/Keywords: Organofluoroborates; Fluoration; Fluorophores; Organofluoroborates; Fluoration; Fluorophores
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APA (6th Edition):
Sadek, O. (2018). Exploiting boron-fluorine bonds for fluorination and synthesis of potential bi-modal imaging agents : Exploitation du couple bore-fluor pour la fluoration et la synthèse d'agnet d'imagerie bimodaux potentiels. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2018TOU30141
Chicago Manual of Style (16th Edition):
Sadek, Omar. “Exploiting boron-fluorine bonds for fluorination and synthesis of potential bi-modal imaging agents : Exploitation du couple bore-fluor pour la fluoration et la synthèse d'agnet d'imagerie bimodaux potentiels.” 2018. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed January 15, 2021.
http://www.theses.fr/2018TOU30141.
MLA Handbook (7th Edition):
Sadek, Omar. “Exploiting boron-fluorine bonds for fluorination and synthesis of potential bi-modal imaging agents : Exploitation du couple bore-fluor pour la fluoration et la synthèse d'agnet d'imagerie bimodaux potentiels.” 2018. Web. 15 Jan 2021.
Vancouver:
Sadek O. Exploiting boron-fluorine bonds for fluorination and synthesis of potential bi-modal imaging agents : Exploitation du couple bore-fluor pour la fluoration et la synthèse d'agnet d'imagerie bimodaux potentiels. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2018. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2018TOU30141.
Council of Science Editors:
Sadek O. Exploiting boron-fluorine bonds for fluorination and synthesis of potential bi-modal imaging agents : Exploitation du couple bore-fluor pour la fluoration et la synthèse d'agnet d'imagerie bimodaux potentiels. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2018. Available from: http://www.theses.fr/2018TOU30141
Boise State University
2.
Bui, Hieu Trung.
Nanoscale Photonic Devices Fabricated Using DNA Nanostructures.
Degree: 2011, Boise State University
URL: https://scholarworks.boisestate.edu/td/203
► In the field of DNA nanotechnology, self-assembly is being advanced as the key technology for the creation of nanoscale structures. Popular and effective DNA nanotechnology…
(more)
▼ In the field of DNA nanotechnology, self-assembly is being advanced as the key technology for the creation of nanoscale structures. Popular and effective DNA nanotechnology methods of producing nanoscale structures are branched DNA junctions and DNA origami. DNA nanostructures have recently been employed as scaffolds for the bottom-up arrangement of proteins, as well as semiconductor and metallic nanoparticles, with nanometer precision. Such structures are expected to exhibit unique optical properties and may enable new photonic devices. Conversely, the majority of photonic devices for optical waveguide are fabricated using top-down processes. However, the cost and controllability of complex nanostructures using top-down processes imposes significant challenges. As an alternative to top-down processes, work will be presented demonstrating the use of DNA self-assembly processes to fabricate nanoscale photonic devices for optical waveguide. To fabricate photonic devices using DNA self-assembly, DNA nanostructures were used as scaffolds to configure light emitting molecules so as to create a near-field energy transfer waveguide. Spectrophotometry was used to characterize the device operation. The spectral results indicate that DNA nanostructures functionalized with light emitting molecules can transfer energy through a three molecule system with 28% efficiency, demonstrating the potential of using DNA nanostructures for future photonic devices for optical waveguide.
Subjects/Keywords: DNA nanostructures; FRET; Fluorophores; Semiconductor nanoparticles
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APA (6th Edition):
Bui, H. T. (2011). Nanoscale Photonic Devices Fabricated Using DNA Nanostructures. (Thesis). Boise State University. Retrieved from https://scholarworks.boisestate.edu/td/203
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bui, Hieu Trung. “Nanoscale Photonic Devices Fabricated Using DNA Nanostructures.” 2011. Thesis, Boise State University. Accessed January 15, 2021.
https://scholarworks.boisestate.edu/td/203.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bui, Hieu Trung. “Nanoscale Photonic Devices Fabricated Using DNA Nanostructures.” 2011. Web. 15 Jan 2021.
Vancouver:
Bui HT. Nanoscale Photonic Devices Fabricated Using DNA Nanostructures. [Internet] [Thesis]. Boise State University; 2011. [cited 2021 Jan 15].
Available from: https://scholarworks.boisestate.edu/td/203.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bui HT. Nanoscale Photonic Devices Fabricated Using DNA Nanostructures. [Thesis]. Boise State University; 2011. Available from: https://scholarworks.boisestate.edu/td/203
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
3.
Meng, Qianli.
Design, Synthesis, Characterization and Evaluation of Near Infrared BODIPY Dyes for Various Applications.
Degree: PhD, Chemistry, 2017, Louisiana State University
URL: etd-04072017-160920
;
https://digitalcommons.lsu.edu/gradschool_dissertations/4439
► 4,4-Difluoro-4-bora-3a,4a-diaza-5-indacene (BODIPY) have drawn a large number of research attention, for its numerous useful application in the fields of material and biology. Small modification of…
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▼ 4,4-Difluoro-4-bora-3a,4a-diaza-5-indacene (BODIPY) have drawn a large number of research attention, for its numerous useful application in the fields of material and biology. Small modification of this well-known fluophore has been shown to optimize its photophysical and chemical properties. This work mainly focuses on functionalization of BODIPY cores with halogenated atoms, particularly iodine, and their subsequent post-functionalization reactions, mainly cross-coupling reaction and substitutions, for various further applications.
Chapter 1 of this dissertation gives a general overview of BODIPYs including their synthetic strategies, post-functionalization, and how BODIPYs are utilized in many biological applications.
Chapter 2 mainly focuses on the synthesis and characterization of 3,5-diiodo-BODIPYs’ and 2,3,5,6-tetraiodo-BODIPY’s cross coupling products, together with the best coupling reaction methodology developments.
Chapter 3 presents various aromatic substitution reactions of 3,5-diiodo-BODIPYs and 2,3,5,6-tetraiodo-BODIPY. The methods are developed with the best solvents, temperature and equivalents of nucleophiles. Due to the high reactivity of the 3,5-iodines, 123I radio labeling reactions were also attempted to produce 123I exchanged BODIPYs within one hour.
Chapter 4 investigates the aromatization reactions on â,â’-bicyclo-BODIPYs, and a full scan of all 3,5-substited groups shows that only electron withdrawing groups are stable during the aromatization reactions. Based on this conclusion, a bis(isothiocyanate)-BODIPY that emits in the near-IR region was prepared and conjugated to an EGFR-L1 peptide.
Chapter 5 reports the synthesis of nitrophenyl BODIPYs, and their electrochemical properties were studied, in collaboration with Dr. Karl Kadish group from University of Houston. In the reductive area, reversible peaks are found, which are corresponded to the reductive reactions on the nitrophenyl units.
Subjects/Keywords: BODIPY Dyes; Fluorophores; Near-Infrared; Photophysical Properties
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APA (6th Edition):
Meng, Q. (2017). Design, Synthesis, Characterization and Evaluation of Near Infrared BODIPY Dyes for Various Applications. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-04072017-160920 ; https://digitalcommons.lsu.edu/gradschool_dissertations/4439
Chicago Manual of Style (16th Edition):
Meng, Qianli. “Design, Synthesis, Characterization and Evaluation of Near Infrared BODIPY Dyes for Various Applications.” 2017. Doctoral Dissertation, Louisiana State University. Accessed January 15, 2021.
etd-04072017-160920 ; https://digitalcommons.lsu.edu/gradschool_dissertations/4439.
MLA Handbook (7th Edition):
Meng, Qianli. “Design, Synthesis, Characterization and Evaluation of Near Infrared BODIPY Dyes for Various Applications.” 2017. Web. 15 Jan 2021.
Vancouver:
Meng Q. Design, Synthesis, Characterization and Evaluation of Near Infrared BODIPY Dyes for Various Applications. [Internet] [Doctoral dissertation]. Louisiana State University; 2017. [cited 2021 Jan 15].
Available from: etd-04072017-160920 ; https://digitalcommons.lsu.edu/gradschool_dissertations/4439.
Council of Science Editors:
Meng Q. Design, Synthesis, Characterization and Evaluation of Near Infrared BODIPY Dyes for Various Applications. [Doctoral Dissertation]. Louisiana State University; 2017. Available from: etd-04072017-160920 ; https://digitalcommons.lsu.edu/gradschool_dissertations/4439
University of Miami
4.
Sansalone, Lorenzo.
Photonic Strategies for Bioimaging and Modulation of Biological Systems.
Degree: PhD, Chemistry (Arts and Sciences), 2018, University of Miami
URL: https://scholarlyrepository.miami.edu/oa_dissertations/2020
► The last two decades have seen how the development of photoactivatable fluorophores significantly implemented imaging and spectroscopic techniques applied to the “biological world”. In fact,…
(more)
▼ The last two decades have seen how the development of photoactivatable
fluorophores significantly implemented imaging and spectroscopic techniques applied to the “biological world”. In fact, molecules that switch from a nonemissive to an emissive state upon illumination at an activating wavelength (λac) and then emit after irradiation at an exciting wavelength (λexc) opened a new field of research and, beside the generation of super resolved subdiffracted images, they permit the monitoring of dynamic processes in real time. In the context of our work, an oxazine-based molecular switch has been designed to generate photoactivatable
fluorophores suitable for bioimaging. The second and third chapters of this thesis focus on the development of a new class of NIR photoactivatable
fluorophores based on a 2-Nitrobenzyl photoswitch. The best representative of the series has been tested to monitor dynamic cellular processes in Drosophila melanogaster embryos. In the last chapter, the very same phototrigger has been used to generate a light activatable version of Diazepam (Prodrug of Diazepam) that was tested on the locomotor behavior of Zebrafish larvae.
Advisors/Committee Members: Francisco M. Raymo, Roger M. Leblanc, James N. Wilson, James D. Baker.
Subjects/Keywords: NIR Fluorophores; NIR Photoactivatable Fluorophores; Bioimaging; Far-red emission; Near-Infrared emission; Drosophila melanogaster dynamics
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APA (6th Edition):
Sansalone, L. (2018). Photonic Strategies for Bioimaging and Modulation of Biological Systems. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/2020
Chicago Manual of Style (16th Edition):
Sansalone, Lorenzo. “Photonic Strategies for Bioimaging and Modulation of Biological Systems.” 2018. Doctoral Dissertation, University of Miami. Accessed January 15, 2021.
https://scholarlyrepository.miami.edu/oa_dissertations/2020.
MLA Handbook (7th Edition):
Sansalone, Lorenzo. “Photonic Strategies for Bioimaging and Modulation of Biological Systems.” 2018. Web. 15 Jan 2021.
Vancouver:
Sansalone L. Photonic Strategies for Bioimaging and Modulation of Biological Systems. [Internet] [Doctoral dissertation]. University of Miami; 2018. [cited 2021 Jan 15].
Available from: https://scholarlyrepository.miami.edu/oa_dissertations/2020.
Council of Science Editors:
Sansalone L. Photonic Strategies for Bioimaging and Modulation of Biological Systems. [Doctoral Dissertation]. University of Miami; 2018. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/2020
5.
Lorne, Thomas.
Contribution of inelastic neutron scattering to the characterization of the grafting of fluorophores onto double-walled carbon nanotubes : Contribution de la diffusion inelastique de neutron à la caractérisation du greffage de fluorophores sur des nanotubes de carbone biparois.
Degree: Docteur es, Sciences et génie des matériaux, 2017, Université Toulouse III – Paul Sabatier
URL: http://www.theses.fr/2017TOU30363
► Face à la recrudescence de l'utilisation des nanotubes de carbone (NTCs) pour des applications de pointe, leur impact potentiel sur la santé et sur l'environnement…
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▼ Face à la recrudescence de l'utilisation des nanotubes de carbone (NTCs) pour des applications de pointe, leur impact potentiel sur la santé et sur l'environnement est devenu une question centrale. Afin d'évaluer les risques liés à l'exposition accidentelle des êtres-vivants à ces nanoparticules de nombreuses études de toxicité ont été réalisées. Pour certaines, il est important de pouvoir déterminer précisément où et comment les NTCs s'accumulent dans les organismes aquatiques ou dans les cellules. Un des moyens habituel pour réaliser de telles expériences consiste à fonctionnaliser les NTCs avec des molécules fluorescentes, qui seront par la suite mises en évidence sous un rayonnement de longueur d'onde appropriée, permettant ainsi de localiser les nanotubes. Malgré le fait que cette technique soit bon marché et facilement mise en œuvre, elle souffre d'un défaut majeur: elle présuppose en effet que les molécules fluorescentes resteront attachées aux nanotubes de façon définitive. Cependant, cette supposition peut être sérieusement questionnée car les
fluorophores possèdent généralement un ou plusieurs cycles aromatiques pouvant facilement conduire à l'adsorption de ces derniers sur les parois des NTCs. Dès lors, une fois que les NTCs atteignent l'environnement chimique complexe d'une cellule, on peut s'attendre à une désorption des molécules fluorescentes, pouvant de fait conduire à de mauvaises interprétations des résultats expérimentaux. Il est par conséquent essentiel d'évaluer l'efficacité du protocole de greffage et de déterminer les différentes proportions de molécules greffées de façon covalente et non-covalente (adsorbées). Bien entendu, la fonctionnalisation des NTCs étant une problématique clef dans le développement de leurs applications en science des matériaux, les questions soulevées ici dépassent le simple cadre de la santé et de l'environnement. Pour y répondre, nous avons décidé de nous intéresser à la fonctionnalisation de nanotubes de carbone double-parois (DWNTs) par deux molécules fluorescentes, l'Isothiocyanate de Fluorescéine (FITC) et la cyanine 5Me(Net2)2. Nous avons, pour ce faire, réaliser le greffage des
fluorophores sur des DWNTs oxydés purifiés à l'aide d'un procédé de fonctionnalisation en 3 étapes. Enfin, nous avons caractérisé nos échantillons au moyen de deux techniques différentes de spectroscopie, la spectroscopie de photoélectron X (XPS) et la spectroscopie de diffusion inélastique de neutrons (INS). En complément de la spectroscopie de neutrons, nous nous sommes appuyés sur des simulations numériques, telle que la théorie de la fonctionnelle de la densité (DFT) pour les analyses de nos données expérimentales. Les résultats ainsi obtenus, ont permis de montrer que, contrairement à ce qui était communément attendu lors d'un greffage covalent, une part non négligeable des marqueurs fluorescent restait adsorbée à la surface des NTCs, et ce, en dépit de lavages consciencieux par des solvants appropriés. La présence de ces marqueurs fluorescents adsorbés montre, dans le cadre des…
Advisors/Committee Members: Flahaut, Emmanuel (thesis director), Jimenez-Ruiz, Monica (thesis director).
Subjects/Keywords: Neutron; Nanotubes de carbone; Fluorophores; Greffage; Spectroscopie; Neutron; Carbon nanotubes; Fluorophores; Grafting; Spectroscopy
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APA ·
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APA (6th Edition):
Lorne, T. (2017). Contribution of inelastic neutron scattering to the characterization of the grafting of fluorophores onto double-walled carbon nanotubes : Contribution de la diffusion inelastique de neutron à la caractérisation du greffage de fluorophores sur des nanotubes de carbone biparois. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2017TOU30363
Chicago Manual of Style (16th Edition):
Lorne, Thomas. “Contribution of inelastic neutron scattering to the characterization of the grafting of fluorophores onto double-walled carbon nanotubes : Contribution de la diffusion inelastique de neutron à la caractérisation du greffage de fluorophores sur des nanotubes de carbone biparois.” 2017. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed January 15, 2021.
http://www.theses.fr/2017TOU30363.
MLA Handbook (7th Edition):
Lorne, Thomas. “Contribution of inelastic neutron scattering to the characterization of the grafting of fluorophores onto double-walled carbon nanotubes : Contribution de la diffusion inelastique de neutron à la caractérisation du greffage de fluorophores sur des nanotubes de carbone biparois.” 2017. Web. 15 Jan 2021.
Vancouver:
Lorne T. Contribution of inelastic neutron scattering to the characterization of the grafting of fluorophores onto double-walled carbon nanotubes : Contribution de la diffusion inelastique de neutron à la caractérisation du greffage de fluorophores sur des nanotubes de carbone biparois. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2017. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2017TOU30363.
Council of Science Editors:
Lorne T. Contribution of inelastic neutron scattering to the characterization of the grafting of fluorophores onto double-walled carbon nanotubes : Contribution de la diffusion inelastique de neutron à la caractérisation du greffage de fluorophores sur des nanotubes de carbone biparois. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2017. Available from: http://www.theses.fr/2017TOU30363
Université de Grenoble
6.
Eucat, Gwenaelle.
Ingénierie moléculaire de nouveaux émetteurs à l'état solide et élaboration de nanoparticules coeur-coquilles pour l'imagerie médicale : Molecular engineering of new organic fluorophores and encapsulation in a sol-gel shell for medical imaging.
Degree: Docteur es, Chimie organique, 2014, Université de Grenoble
URL: http://www.theses.fr/2014GRENV035
► Une ingénierie moléculaire a été menée. On a ainsi obtenu des fluorophores émettant, à l'état solide, dans le rouge et le proche infrarouge avec des…
(more)
▼ Une ingénierie moléculaire a été menée. On a ainsi obtenu des fluorophores émettant, à l'état solide, dans le rouge et le proche infrarouge avec des rendements quantiques performants. Nous nous sommes particulièrement intéressés à des petites molécules de type push-pull, facile à synthétiser, permettant ainsi d'obtenir un grand nombre de molécules indispensable pour cette ingénierie moléculaire. Il a notamment été constaté que certaines règles établies en solution sont également valables à l'état solide. Ensuite, les chromophores répondant au cahier des charges fixé par la méthode de séchage par spray ont été sélectionnés et encapsulés, d'une part, en couche mince sol-gel afin d'observer leur comportement en milieu confinée. D'autre part, pour ceux ayant un bon comportement en matrice sol-gel (contrôlé par spectroscopie de fluorescence à un photon), ils ont été insérés dans une coquille du même type. Les conditions d'élaboration pour chaque chromophore ont été optimisées en se basant, essentiellement, sur la technique de microscopie électronique à balayage. Nous avons, en particulier, pu démontrer que l'étape d'encapsulation en couche mince sol-gel, était une très bonne technique, rapide et facile à mettre en œuvre, pour s'assurer que les composés ne souffraient ni de polymorphisme ni de protonation dans une matrice sol-gel avant la synthèse de nanoparticules cœur-coquille. Enfin, une étape de fonctionnalisation et d'ajout d'agent de ciblage permettra d'effectuer des premiers tests in vivo des nanoparticules comme agents imageant.
A molecular engineering was led. We obtained fluorophores emitting, in the solid state, in the red and the near infrared with efficient quantum yields. We were particularly interested in small push-pull molecules, easy to synthesize, to obtain a large number of molecules essential for this molecular engineering. Especially, it was noticed that certain rules established in solution are also valid in the solid state. Then, chromophores which correspond to the specifications fixed by the spray drying method was selected and encapsulated, on one hand, in a sol-gel thin-layer to observe their behavior in an environment confined. On the other hand, for those having a good behavior in the sol-gel matrix (controlled by one photon fluorescence spectroscopy), they were confined in a sol-gel shell of the same type. Elaboration's conditions for every compound were optimized, essentially, with the technique of scanning electron microscopy. We demonstrated that the encapsulation step in a sol-gel thin-layer was a very good technique, fast and easy to operate, to make sure that compounds had neither polymorphism nor protonation in a sol-gel matrix before the synthesis of the nanoparticles. Finally, a functionalization step and addition of new targeting functions will allow making first in vivo tests of nanoparticles as biological labels.
Advisors/Committee Members: Ibanez, Alain (thesis director), Andraud, Chantal (thesis director).
Subjects/Keywords: Nanocristallisation; Fluorescence à l'état solide; Fluorophores organiques; Imagerie médicale; Nanocrystallization; Fluorescent in the solid state; Organic fluorophores; Biological labels; 540
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APA ·
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APA (6th Edition):
Eucat, G. (2014). Ingénierie moléculaire de nouveaux émetteurs à l'état solide et élaboration de nanoparticules coeur-coquilles pour l'imagerie médicale : Molecular engineering of new organic fluorophores and encapsulation in a sol-gel shell for medical imaging. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2014GRENV035
Chicago Manual of Style (16th Edition):
Eucat, Gwenaelle. “Ingénierie moléculaire de nouveaux émetteurs à l'état solide et élaboration de nanoparticules coeur-coquilles pour l'imagerie médicale : Molecular engineering of new organic fluorophores and encapsulation in a sol-gel shell for medical imaging.” 2014. Doctoral Dissertation, Université de Grenoble. Accessed January 15, 2021.
http://www.theses.fr/2014GRENV035.
MLA Handbook (7th Edition):
Eucat, Gwenaelle. “Ingénierie moléculaire de nouveaux émetteurs à l'état solide et élaboration de nanoparticules coeur-coquilles pour l'imagerie médicale : Molecular engineering of new organic fluorophores and encapsulation in a sol-gel shell for medical imaging.” 2014. Web. 15 Jan 2021.
Vancouver:
Eucat G. Ingénierie moléculaire de nouveaux émetteurs à l'état solide et élaboration de nanoparticules coeur-coquilles pour l'imagerie médicale : Molecular engineering of new organic fluorophores and encapsulation in a sol-gel shell for medical imaging. [Internet] [Doctoral dissertation]. Université de Grenoble; 2014. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2014GRENV035.
Council of Science Editors:
Eucat G. Ingénierie moléculaire de nouveaux émetteurs à l'état solide et élaboration de nanoparticules coeur-coquilles pour l'imagerie médicale : Molecular engineering of new organic fluorophores and encapsulation in a sol-gel shell for medical imaging. [Doctoral Dissertation]. Université de Grenoble; 2014. Available from: http://www.theses.fr/2014GRENV035
7.
Pagano, Paolo.
Design and synthesis of ultra-bright organic nanoparticles (ONPs) for bioimaging : Elaboration et caractérisation de nanoparticules ultra-brillantes, fonctionnalisées et biocompatibles, pour applications en biologie et en médecine.
Degree: Docteur es, Chimie organique, 2017, Bordeaux
URL: http://www.theses.fr/2017BORD0634
► L’utilisation de nano-objets luminescents en milieu biologique est devenue très répandue, notamment en vue d’applications biomédical est elles que l’imagerie, la thérapie et le diagnostic.…
(more)
▼ L’utilisation de nano-objets luminescents en milieu biologique est devenue très répandue, notamment en vue d’applications biomédical est elles que l’imagerie, la thérapie et le diagnostic. Jusqu’à récemment, les principaux travaux réalisés dans ce domaine concernaient les nanoparticules de silice dopées ou fonctionnalisées avec des molécules organiques, les nanoparticules d’or et les nanoparticules semi-conductrices (quantum dots, i.e., QDs). Toutefois, un certain nombre de limitations demeurent pour les applications dans le domaine du vivant, en lien notamment avec des problèmes de stabilité, de biocompatibilité et de toxicité ou encore de biodégradabilité. En parallèle,un certain nombre de molécules organiques fluorescentes non-toxiques ont été utilisées comme sondes fluorescentes en milieu biologique, mais leur brillance demeure limitée. L’idée directrice de la thèse est de concevoir et synthétiser de nouveaux chromophores organiques présentant une émission modulable (du visible au proche infrarouge) et adaptés à la préparation de nanoparticules organiques fluorescentes (FONs) combinant à la fois une brillance extrêmement élevée, une excellente stabilité colloïdale et une photostabilité adaptée à leur utilisation en imagerie in vitro et in vivo. De tels nanoobjets ultra-brillants pourraient alors représenter une alternative très intéressante aux nanoparticules actuellement les plus utilisées en imagerie de fluorescence du vivant (QDs). Le manuscrit décrit la synthèse et les propriétés de plusieurs classes de molécules fluorescentes spécifiquement conçues pour former des telles FONS par auto-assemblage dans l’eau. La préparation de ces FONs est présentée et leurs propriétés étudiées et discutées. Enfin des applications concrètes en bio-imagerie sont présentées.
Nowadays the use of bright luminescent nano-objects in biological environment is a topic that is gaining more and more importance, especially for biomedical applications such as imaging, the rapyand diagnostic. So far, numerous studies have been conducted with gold nanoparticles, silica nanoparticles (doped or functionalized with organic molecules), as well as semiconductor nanoparticles (quantum dots, i.e., QDs). However, most of these nanoparticles suffer from drawbacks (in terms of stability, biocompatibility, eco-toxicity or degradability). On the other hand, several nontoxic fluorescent molecular probes have been widely used, but most of the time their brightness remain modest in biological environments compared to QDs. Our idea is to engineer new organicchromophores with tunable emission wavelength (from visible to near infrared) for further preparation of organic fluorescent nanoparticles (so called FONs) that display giant one-photon and two-photonbrightness, as well as good colloidal and chemical stability, and suitable photostability for in vitro andin vivo imaging. As such, these FONs would represent interesting alternatives to QDs for use in bioimaging. This manuscript describes the synthesis and characterization of new classes of fluorescent…
Advisors/Committee Members: Blanchard-Desce, Mireille (thesis director).
Subjects/Keywords: Absorption à deux photons; Fluorophores; Nanoparticules organiques; Imagerie biologique; Two-photon absorption; Fluorophores; Organic nanoparticles; Bioimaging
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APA (6th Edition):
Pagano, P. (2017). Design and synthesis of ultra-bright organic nanoparticles (ONPs) for bioimaging : Elaboration et caractérisation de nanoparticules ultra-brillantes, fonctionnalisées et biocompatibles, pour applications en biologie et en médecine. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2017BORD0634
Chicago Manual of Style (16th Edition):
Pagano, Paolo. “Design and synthesis of ultra-bright organic nanoparticles (ONPs) for bioimaging : Elaboration et caractérisation de nanoparticules ultra-brillantes, fonctionnalisées et biocompatibles, pour applications en biologie et en médecine.” 2017. Doctoral Dissertation, Bordeaux. Accessed January 15, 2021.
http://www.theses.fr/2017BORD0634.
MLA Handbook (7th Edition):
Pagano, Paolo. “Design and synthesis of ultra-bright organic nanoparticles (ONPs) for bioimaging : Elaboration et caractérisation de nanoparticules ultra-brillantes, fonctionnalisées et biocompatibles, pour applications en biologie et en médecine.” 2017. Web. 15 Jan 2021.
Vancouver:
Pagano P. Design and synthesis of ultra-bright organic nanoparticles (ONPs) for bioimaging : Elaboration et caractérisation de nanoparticules ultra-brillantes, fonctionnalisées et biocompatibles, pour applications en biologie et en médecine. [Internet] [Doctoral dissertation]. Bordeaux; 2017. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2017BORD0634.
Council of Science Editors:
Pagano P. Design and synthesis of ultra-bright organic nanoparticles (ONPs) for bioimaging : Elaboration et caractérisation de nanoparticules ultra-brillantes, fonctionnalisées et biocompatibles, pour applications en biologie et en médecine. [Doctoral Dissertation]. Bordeaux; 2017. Available from: http://www.theses.fr/2017BORD0634
Penn State University
8.
Mottram, Laurie Frances.
Design, Synthesis and Biological Evaluation of cell-permeable small molecule probes
.
Degree: 2008, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/7918
► Molecular probes derived from fluorescein are widely used as tools for studies of cellular biology. This classic green fluorophore is particularly suited for cellular analysis…
(more)
▼ Molecular probes derived from fluorescein are widely used as tools for studies of cellular biology. This classic green fluorophore is particularly suited for cellular analysis by confocal laser scanning microscopy and flow cytometry due to its excitation maximum at 490 nm, closely matching the 488 nm spectral line of the argon-ion laser. Under physiological conditions (pH = 7.4), fluorescein predominantly exists as a highly hydrophilic dianion exhibiting excellent quantum yield. However, protonation of fluorescein to the monoanion is observed with the relatively high pKa of 6.5, rendering this dye much less fluorescent in acidic solutions. Additionally, fluorescein is relatively susceptible to photobleaching. Oregon Green, a more acidic 2’, 7’-difluoro derivative of fluorescein, was developed as a less pH-sensitive fluorophore. The appended fluorine atoms reduce the pKa of this dye to 4.8, substantially improving both photostability and fluorescence at low pH. Another more hydrophobic fluorescein analogue termed Tokyo Green was recently reported that replaces the carboxylate with a methyl group. This structural modification yields a highly fluorescent monoanion. We report a novel fluorophore termed Pennsylvania Green that combines the pH-insensitivity and photostability of Oregon Green with the hydrophobicity of Tokyo Green. To demonstrate the utility of the Pennsylvania Green fluorophore, we compared cellular membrane probes derived from 4-carboxy Tokyo Green and 4-carboxy Pennsylvania Green. Because of its lower pKa, only the Pennsylvania Green-derived probe enables facile visualization of acidic endosomes within living mammalian cells.
We also report an improved synthesis of the Pennsylvania Green fluorophore and its related 4-carboxy derivative. 4-Carboxy-Pennsylvania Green was prepared from 4-iodo-3-methyl-benzoic acid methyl ester in three steps and 32% overall yield. This new synthesis is a notable improvement over our original methods. Chinese hamster ovary cells expressing O6-alkylguanine-DNA alkyltransferase fusion proteins were treated with Pennsylvania Green and Oregon Green linked to O6-benzylguanine (SNAP-Tag substrates). Analysis by confocal laser scanning microscopy revealed that Pennsylvania Green derivatives are substantially more cell permeable than analogous Orgeon Green Probes.
Using the improved synthetic methods identified for the preparation of Pennsylvania Green, four novel red
fluorophores were designed and synthesized: Penn Fluor 550, Penn Fluor 555, Penn Fluor 576 and Penn Fluor 609. The ability of biotinylated derivatives of these new
fluorophores to image strepavidin fusion proteins in living mammalian cells was investigated.
As a non-fluorescent molecular probe of potential drug targets and the steroid chaperone cycle, we synthesized dimeric molecules design to control estrogen receptor and heat shock protein 90 in living cells. Heat shock protein 90 (Hsp90) is an abundant ATP-dependent cellular chaperone that facilitates the folding of estrogen receptors (ERs) and other oncogenic…
Advisors/Committee Members: Blake R Peterson, Committee Chair/Co-Chair, Philip C Bevilaqua, Committee Member, Ken S Feldman, Committee Member, Avery August, Committee Member.
Subjects/Keywords: fluorescent probes; molecular probes; fluorophores
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APA (6th Edition):
Mottram, L. F. (2008). Design, Synthesis and Biological Evaluation of cell-permeable small molecule probes
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/7918
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mottram, Laurie Frances. “Design, Synthesis and Biological Evaluation of cell-permeable small molecule probes
.” 2008. Thesis, Penn State University. Accessed January 15, 2021.
https://submit-etda.libraries.psu.edu/catalog/7918.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mottram, Laurie Frances. “Design, Synthesis and Biological Evaluation of cell-permeable small molecule probes
.” 2008. Web. 15 Jan 2021.
Vancouver:
Mottram LF. Design, Synthesis and Biological Evaluation of cell-permeable small molecule probes
. [Internet] [Thesis]. Penn State University; 2008. [cited 2021 Jan 15].
Available from: https://submit-etda.libraries.psu.edu/catalog/7918.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mottram LF. Design, Synthesis and Biological Evaluation of cell-permeable small molecule probes
. [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/7918
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Miami
9.
Thapaliya, Ek Raj.
Mechanisms to Photoactivate Fluorescence and Strategies for Supramolecular Delivery in Bioimaging Applications.
Degree: PhD, Chemistry (Arts and Sciences), 2017, University of Miami
URL: https://scholarlyrepository.miami.edu/oa_dissertations/1809
► Fluorescence microscopy in combination with photoactivatable fluorophores offers the opportunity to image noninvasively the biological samples in real time with subdiffraction resolution. Photoactivatable fluorophores switch…
(more)
▼ Fluorescence microscopy in combination with photoactivatable
fluorophores offers the opportunity to image noninvasively the biological samples in real time with subdiffraction resolution. Photoactivatable
fluorophores switch from nonemissive state to emissive state upon irradiation with activation wavelength and then emit light after excitation of product at suitable excitation wavelength. The concatenation of a photochemical reaction (activation) with a photophysical process (fluorescence) is therefore responsible for the operating principles of these photoresponsive compounds to switch fluorescence on within a defined region of space at a particular interval of time. In turn, such a spatiotemporal control permits the monitoring of dynamic events in real time and the visualization of samples with subdiffraction resolution. The identification of viable operating principles to photoactivate the fluorescence of organic chromophores is, therefore, essential for the further development of these promising imaging techniques. In this context, we designed two mechanisms for the activation of the fluorescence of anthracene derivatives based on photoinduced retro-cycloadditions and photodecarbonylation. Furthermore, mechanism to photoactivate fluorescence by photodecarbonylation was designed to proceed by autocatalytic photochemical pathway where the photoproduct can sensitize its own formation from the reactant, under illumination at a wavelength capable of exciting both species.
The amphiphilic polymer can encapsulate diversity of hydrophobic guest into their hydrophobic core and transport them into the aqueous phase, thus, can be a valuable delivery vehicle for diversity of biomedical applications. These supramolecular nanocarriers along with covalent integration of
fluorophores in their molecular backbone can be useful to probe them directly into the intracellular space. In this context, we synthesized amphiphilic polymer by covalent integration of either donor or acceptor in their macromolecular backbone. Their photophysical properties both in organic and aqueous phase along with imaging experiment in Hela cells were performed. We further. optimized the brightness of these polymer by keeping compact dimension, with systematic integration of number of
fluorophores in their macromolecular backbone, as a result, these polymers are significant brighter than the model monomers. Such a high brightness level is maintained even after injection of the macromolecular probes in living nematodes, allowing their visualization with a significant improvement in signal-to-noise ratio, relative to the model monomer, and no cytotoxic or behavioral effects. Further, the bioconjugate of this polymer with secondary antibody does not hinder the targeting ability of the former and also can be exploited to stain the tubulin structures of model cells to enable their visualization with optimal signal-to-noise ratios.
We envisaged the protocol to imprint fluorescent pattern on photoresponsive polymer film with mild illumination…
Advisors/Committee Members: Françisco M. Raymo, Burjor Captain, James N. Wilson, Kevin M. Collins.
Subjects/Keywords: Photoactivatable Fluorophores; Autocatalysis; Supramolecular Nanocarriers; Self-assembly; FRET; Bioimaging.
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APA (6th Edition):
Thapaliya, E. R. (2017). Mechanisms to Photoactivate Fluorescence and Strategies for Supramolecular Delivery in Bioimaging Applications. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/1809
Chicago Manual of Style (16th Edition):
Thapaliya, Ek Raj. “Mechanisms to Photoactivate Fluorescence and Strategies for Supramolecular Delivery in Bioimaging Applications.” 2017. Doctoral Dissertation, University of Miami. Accessed January 15, 2021.
https://scholarlyrepository.miami.edu/oa_dissertations/1809.
MLA Handbook (7th Edition):
Thapaliya, Ek Raj. “Mechanisms to Photoactivate Fluorescence and Strategies for Supramolecular Delivery in Bioimaging Applications.” 2017. Web. 15 Jan 2021.
Vancouver:
Thapaliya ER. Mechanisms to Photoactivate Fluorescence and Strategies for Supramolecular Delivery in Bioimaging Applications. [Internet] [Doctoral dissertation]. University of Miami; 2017. [cited 2021 Jan 15].
Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1809.
Council of Science Editors:
Thapaliya ER. Mechanisms to Photoactivate Fluorescence and Strategies for Supramolecular Delivery in Bioimaging Applications. [Doctoral Dissertation]. University of Miami; 2017. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1809
University of Houston
10.
-8238-9376.
Linker Functionalization of a Bivalent Ligand for the Study of the 5-HT2AR/ 5-HT2CR Heterodimer.
Degree: MS, Chemistry, 2017, University of Houston
URL: http://hdl.handle.net/10657/4799
► G-protein-coupled receptors (GPCRs) are important pharmaceutical targets. In recent years, it has been found that GPCRs can function as homo- and hetero- dimers. The serotonin…
(more)
▼ G-protein-coupled receptors (GPCRs) are important pharmaceutical targets. In recent years, it has been found that GPCRs can function as homo- and hetero- dimers. The serotonin receptors 5-HT2AR and 5-HT2CR have been shown to form a heterodimer. These two receptors regulate impulsivity and cue reactivity, two factors that can lead to drug addiction relapse. A synergistic effect of the 5-HT2AR antagonist M100907 and the 5-HT2CR agonist WAY163909 in the suppression of impulsivity and cue reactivity has been reported. A bivalent ligand containing these two pharmacophores, linked by a polyethylene glycol chain and a triazole, has been developed.
In this thesis, attempts to functionalize the linker portion of the bivalent ligand for the addition of a fluorophore are reported. The first approach was to functionalize the triazole portion of the linker via the iodoalkyne-azide cycloaddition reaction; however, it was determined that this method was unsuitable due to challenges with purity and yield. The second approach was to functionalize the polyethylene glycol portion of the linker by replacing an oxygen atom with a nitrogen atom, allowing for tertiary substitution at the nitrogen. A linker was synthesized containing a tertiary amine in which an ester side-chain was included. This ester serves as a handle for incorporation of a fluorophore via amide coupling. A fluorophore-tagged bivalent ligand will open the door to cellular imaging of the 5-HT2AR/5-HT2CR GPCR heterodimer.
Advisors/Committee Members: Gilbertson, Scott R. (advisor), Coltart, Don M. (committee member), Daugulis, Olafs (committee member), Guloy, Arnold M. (committee member), Cuny, Gregory D. (committee member).
Subjects/Keywords: Serotonin; Bivalent ligand; 5-HT2AR antagonist; 5-HT2CR agonist; Fluorophores
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APA ·
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APA (6th Edition):
-8238-9376. (2017). Linker Functionalization of a Bivalent Ligand for the Study of the 5-HT2AR/ 5-HT2CR Heterodimer. (Masters Thesis). University of Houston. Retrieved from http://hdl.handle.net/10657/4799
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Chicago Manual of Style (16th Edition):
-8238-9376. “Linker Functionalization of a Bivalent Ligand for the Study of the 5-HT2AR/ 5-HT2CR Heterodimer.” 2017. Masters Thesis, University of Houston. Accessed January 15, 2021.
http://hdl.handle.net/10657/4799.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
MLA Handbook (7th Edition):
-8238-9376. “Linker Functionalization of a Bivalent Ligand for the Study of the 5-HT2AR/ 5-HT2CR Heterodimer.” 2017. Web. 15 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
-8238-9376. Linker Functionalization of a Bivalent Ligand for the Study of the 5-HT2AR/ 5-HT2CR Heterodimer. [Internet] [Masters thesis]. University of Houston; 2017. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10657/4799.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Council of Science Editors:
-8238-9376. Linker Functionalization of a Bivalent Ligand for the Study of the 5-HT2AR/ 5-HT2CR Heterodimer. [Masters Thesis]. University of Houston; 2017. Available from: http://hdl.handle.net/10657/4799
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
University of Houston
11.
Lim, Jaebum 1978-.
Benzobisoxazole Cruciforms and Guanosine Derivatives: Syntheses, Structural Analyses, and Optical Properties.
Degree: PhD, Chemistry, 2012, University of Houston
URL: http://hdl.handle.net/10657/550
► This dissertation presents two studies in supramolecular chemistry; one is the chemistry of benzobisoxazole cruciforms in which two linearly conjugated π systems meet at the…
(more)
▼ This dissertation presents two studies in supramolecular chemistry; one is the chemistry of benzobisoxazole cruciforms in which two linearly conjugated π systems meet at the unsaturated center, and the other is that of guanosine derivatives.
Chapter One. This chapter summarizes the previous work on molecular cruciforms: tetrakis(arylethynyl)benzenes, distyrylbis(arylethynyl)benzenes, tetrakis(aryl-vinyl)benzenes, and tetraethynylethenes.
Chapter Two. The synthesis, optical properties, and computational calculation of benzobisoxazole-based molecular cruciforms are presented. The potential use of donor/acceptor substituted cruciforms as sensors is also discussed, and optical response of cruciform compounds to different concentration of trifluoroacetic acid in the liquid state is presented.
Chapter Three. A method for the identification method of carboxylic acids, organoboronic acids, phenols, amines, ureas, and tetrabutylammonium salts is described using one of donor/acceptor substituted benzobisoxazole-cruciforms as the sensor.
Chapter Four. Structural study of benzobisoxazole cruciforms is presented: their solid state structures, obtained by X-ray crystallography, are presented.
Chapter Five. The synthesis and structural analysis of 8-arylethynyl substituted guanosine derivatives is presented. The structural effects of this substitution, in comparison to the previous work in guanosine chemistry, is also discussed.
Advisors/Committee Members: Miljanić, Ognjen Š. (advisor), Lee, T. Randall (committee member), Gilbertson, Scott R. (committee member), Jacobson, Allan J. (committee member), Brankovic, Stanko R. (committee member).
Subjects/Keywords: Self-assembly; Benzobisoxazole; Cruciform molecules; Fluorophores; Sensors; Guanosine; Chemistry, Organic
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Lim, J. 1. (2012). Benzobisoxazole Cruciforms and Guanosine Derivatives: Syntheses, Structural Analyses, and Optical Properties. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/550
Chicago Manual of Style (16th Edition):
Lim, Jaebum 1978-. “Benzobisoxazole Cruciforms and Guanosine Derivatives: Syntheses, Structural Analyses, and Optical Properties.” 2012. Doctoral Dissertation, University of Houston. Accessed January 15, 2021.
http://hdl.handle.net/10657/550.
MLA Handbook (7th Edition):
Lim, Jaebum 1978-. “Benzobisoxazole Cruciforms and Guanosine Derivatives: Syntheses, Structural Analyses, and Optical Properties.” 2012. Web. 15 Jan 2021.
Vancouver:
Lim J1. Benzobisoxazole Cruciforms and Guanosine Derivatives: Syntheses, Structural Analyses, and Optical Properties. [Internet] [Doctoral dissertation]. University of Houston; 2012. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10657/550.
Council of Science Editors:
Lim J1. Benzobisoxazole Cruciforms and Guanosine Derivatives: Syntheses, Structural Analyses, and Optical Properties. [Doctoral Dissertation]. University of Houston; 2012. Available from: http://hdl.handle.net/10657/550
University of Debrecen
12.
Bartels, Barima Osei Yaw.
Solvatochromic investigation of heteroaromatic fluorophores
.
Degree: DE – Természettudományi és Technológiai Kar – Kémiai Intézet, University of Debrecen
URL: http://hdl.handle.net/2437/215478
► The aim of this thesis work was to investigate the solvatochromic properties of two molecules, namely 6-hydroxy-2-phenylpyridazin-3(2H)-one (A1) and N-(5,6-diphenylfuro[2,3-d]pyrimidin-4-yl)benzamine (A2), received from Ain Shams…
(more)
▼ The aim of this thesis work was to investigate the solvatochromic properties of two molecules, namely 6-hydroxy-2-phenylpyridazin-3(2H)-one (A1) and N-(5,6-diphenylfuro[2,3-d]pyrimidin-4-yl)benzamine (A2), received from Ain Shams University at Roxy-Cairo, Egypt. Theoretical calculations were indicated a high dipole moment for both molecules, therefore a strong solvatochromic behavior was expected because of the easy polarizability.
Advisors/Committee Members: Nagy, Miklós (advisor).
Subjects/Keywords: heteroaromatic fluorophores;
Solvatochromic;
investigation
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APA (6th Edition):
Bartels, B. O. Y. (n.d.). Solvatochromic investigation of heteroaromatic fluorophores
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/215478
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bartels, Barima Osei Yaw. “Solvatochromic investigation of heteroaromatic fluorophores
.” Thesis, University of Debrecen. Accessed January 15, 2021.
http://hdl.handle.net/2437/215478.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bartels, Barima Osei Yaw. “Solvatochromic investigation of heteroaromatic fluorophores
.” Web. 15 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Bartels BOY. Solvatochromic investigation of heteroaromatic fluorophores
. [Internet] [Thesis]. University of Debrecen; [cited 2021 Jan 15].
Available from: http://hdl.handle.net/2437/215478.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Council of Science Editors:
Bartels BOY. Solvatochromic investigation of heteroaromatic fluorophores
. [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/215478
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
13.
Mitchell, Amanda.
Development of a Novel Genetically Encoded FRET System Using
the Unnatural Amino Acid Anap.
Degree: MS, Chemistry, 2016, Boston College
URL: http://dlib.bc.edu/islandora/object/bc-ir:107177
► Förster Resonance Energy Transfer (FRET) offers a powerful approach to study biomolecular dynamics in vitro as well as in vivo. The ability to apply FRET…
(more)
▼ Förster Resonance Energy Transfer (FRET) offers a
powerful approach to study biomolecular dynamics in vitro as well
as in vivo. The ability to apply FRET imaging to proteins in living
cells provides an excellent tool to monitor important dynamic
events such as protein conformational changes, protein-protein
interactions, and proteolysis reactions. However, selectively
incorporating two distinct
fluorophores into the target protein(s)
that are capable of FRET interaction within the complex cellular
milieu is challenging. Consequently, terminal fusion to genetically
encoded fluorescent proteins has emerged as the predominant
labeling strategy for FRET studies in vivo. However, a major
limitation of this strategy stems from the large size of the
fluorescent proteins, which may perturb the native properties of
the target, and restricted attachment only to the termini of the
target. We reasoned that using genetically encoded fluorescent
unnatural amino acids would overcome several of these challenges
associated with currently available labeling strategies owing to
their small size and the ability to introduce them site-
specifically and co-translationally. Here, we report the use of the
fluorescent unnatural amino acid “Anap” as a FRET donor with green
and yellow fluorescent protein acceptors. We demonstrate the
utility of this labeling strategy using proteolysis and
conformational change models, and step towards in vivo studies by
further developing a proteolysis system in cell
lysates.
Advisors/Committee Members: Abhishek Chatterjee (Thesis advisor).
Subjects/Keywords: fluorophores; FRET; labeling strategies; resonance energy transfer; unnatural amino acids
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APA ·
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APA (6th Edition):
Mitchell, A. (2016). Development of a Novel Genetically Encoded FRET System Using
the Unnatural Amino Acid Anap. (Masters Thesis). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:107177
Chicago Manual of Style (16th Edition):
Mitchell, Amanda. “Development of a Novel Genetically Encoded FRET System Using
the Unnatural Amino Acid Anap.” 2016. Masters Thesis, Boston College. Accessed January 15, 2021.
http://dlib.bc.edu/islandora/object/bc-ir:107177.
MLA Handbook (7th Edition):
Mitchell, Amanda. “Development of a Novel Genetically Encoded FRET System Using
the Unnatural Amino Acid Anap.” 2016. Web. 15 Jan 2021.
Vancouver:
Mitchell A. Development of a Novel Genetically Encoded FRET System Using
the Unnatural Amino Acid Anap. [Internet] [Masters thesis]. Boston College; 2016. [cited 2021 Jan 15].
Available from: http://dlib.bc.edu/islandora/object/bc-ir:107177.
Council of Science Editors:
Mitchell A. Development of a Novel Genetically Encoded FRET System Using
the Unnatural Amino Acid Anap. [Masters Thesis]. Boston College; 2016. Available from: http://dlib.bc.edu/islandora/object/bc-ir:107177
University of Edinburgh
14.
Norouzi, Neil.
Synthesis and application of novel near infrared cyanine dyes and optical imaging agents.
Degree: PhD, 2014, University of Edinburgh
URL: http://hdl.handle.net/1842/10002
► The use of fluorescent imaging probes for the real time detection of cellular malfunctions, such as enzyme over expression has shown promise. Fluorescent dyes with…
(more)
▼ The use of fluorescent imaging probes for the real time detection of cellular malfunctions, such as enzyme over expression has shown promise. Fluorescent dyes with absorption and emission values below 600 nm are limited in their in vivo applications due to high background auto-fluorescence and low resolution images. Employing near infrared (NIR) fluorophores such as cyanine dyes can overcome this disadvantage. Cyanine dyes can be synthesised using solution or solid-phase techniques with the use of solution phase chemistry allowing for larger scale and higher yielding reactions. Utilising a selection of functional groups and varying polymethine chain lengths a cyanine dye library with tuneable absorption and emission wavelengths was synthesised. This thesis gives the first detailed examples of how modifications on heptamethine cyanine dyes alter their cellular uptake and cellular toxicity. Furthermore, a NIR fluorescent microsphere is reported as well as NIR functionalised microspheres with the ability to be tracked within cells. Additional lines of work involved the synthesis of a fluorescent sensor for the visualisation of bacteria. Aminopeptidases are present within the peptidoglycan cell wall of Gram negative bacteria and therefore can be targeted for real time detection of bacteria to aid in the detection of infectious diseases. A coumarin based probe is reported which detects aminopeptidase in gram negative bacteria in vitro.
Subjects/Keywords: 572; molecular imaging; cyanine dyes; fluorophores; fluorescent; microspheres
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APA (6th Edition):
Norouzi, N. (2014). Synthesis and application of novel near infrared cyanine dyes and optical imaging agents. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/10002
Chicago Manual of Style (16th Edition):
Norouzi, Neil. “Synthesis and application of novel near infrared cyanine dyes and optical imaging agents.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed January 15, 2021.
http://hdl.handle.net/1842/10002.
MLA Handbook (7th Edition):
Norouzi, Neil. “Synthesis and application of novel near infrared cyanine dyes and optical imaging agents.” 2014. Web. 15 Jan 2021.
Vancouver:
Norouzi N. Synthesis and application of novel near infrared cyanine dyes and optical imaging agents. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1842/10002.
Council of Science Editors:
Norouzi N. Synthesis and application of novel near infrared cyanine dyes and optical imaging agents. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/10002
National University of Ireland – Galway
15.
Islam, Muhammad Zakarul.
Studying the fluorescence emission properties of 1,2,3 triazine based fluorophores in polymer thin films
.
Degree: 2017, National University of Ireland – Galway
URL: http://hdl.handle.net/10379/6506
► The application of thin polymeric films for medical purposes is growing very fast. Two of the most important uses of thin polymer films are drug…
(more)
▼ The application of thin polymeric films for medical purposes is growing very fast.
Two of the most important uses of thin polymer films are drug coatings for controlled
release and tissue engineering. Thermoresponsive polymers are extensively used in this
field [1-3]. These polymer thin films are complex in geometry and water uptake into thin
films (during manufacturing or aging) may change their physicochemical properties
limiting their application. One challenge for the use of soft thermoresponsive polymers as
thin films in regulated medical devices and drug delivery systems is their accurate
characterisation on surfaces. A non-contact, non-destructive, method is required for the in
situ analysis of polymer thin films. Spectroscopic techniques, both vibrational (i.e. IR,
Raman) and electronic excitation (UV-Visible, Fluorescence spectroscopy) have received
much attention for the analysis of polymer thin films. Among them fluorescence
spectroscopy has received considerable interest due to its high sensitivity and nondestructive
nature [4]. The use of fluorescence spectroscopy as a potential tool for the rapid,
in situ, and routine analysis of polymer film characterization was investigated in this thesis.
In order to justify the use of 1,2,3- triazine (Tr)
fluorophores for the development
of fluorescence based methods for thickness measurements (of thermoresponsive polymer
thin films), we then investigated the photophysical properties of Tr doped poly N-
(isopropyl acrylamide), PNIPAm (Tr-PNIPAm) thin films. Three derivatives such as 5-
methoxycarbonyl-5-(N-phenylformimidoyl)-2,4,6-triphenyl-2,5-dihydro-1,2,3-triazine
(pTr), hydrolysed form of pTr (hTr), and keto form of triazine (kpTr) were used for this
study. These Tr
fluorophores are hydrophobic and their overall emission is complex and
composed of three emitting conformers. They have a large Stokes shift, and their absorption
and emission properties are unaffected by changing the nature of the solvents [5-7]. We
incorporated these
fluorophores into a thermoresponsive polymer (e.g. PNIPAm) and
investigated the effect of humidity on emission properties. Here we controlled the polymer
environment using a controlled humidity chamber and analysed the emission properties of
the
fluorophores using both steady-state (S-S) and time-resolved fluorescence (TRF)
measurements. The hydrophobic nature of Tr
fluorophores governs its photophysical
properties. The pTr doped PNIPAm (pTr-PNIPAm) thin film shows excellent stability and
was insensitive to changes of microenvironment (i.e. humidity, temperature). The S-S
emission properties showed only minor changes and the variation in the lifetime
measurement was due to refractive index (RI) changes of the polymer matrix. Most
ii
significantly, pTr-PNIPAm thin film also showed unique photostability compared to the
other triazine
fluorophores studies.
In addition, a series of Tr
fluorophores doped polyvinyl alcohol, PVA (Tr-PVA)
thin films were also fabricated and studied to explore the…
Advisors/Committee Members: Ryder, Alan G (advisor).
Subjects/Keywords: Chemistry;
Fluorescence;
Fluorescence emission properties;
Triazine;
Fluorophores;
Polymer thin films
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Islam, M. Z. (2017). Studying the fluorescence emission properties of 1,2,3 triazine based fluorophores in polymer thin films
. (Thesis). National University of Ireland – Galway. Retrieved from http://hdl.handle.net/10379/6506
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Islam, Muhammad Zakarul. “Studying the fluorescence emission properties of 1,2,3 triazine based fluorophores in polymer thin films
.” 2017. Thesis, National University of Ireland – Galway. Accessed January 15, 2021.
http://hdl.handle.net/10379/6506.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Islam, Muhammad Zakarul. “Studying the fluorescence emission properties of 1,2,3 triazine based fluorophores in polymer thin films
.” 2017. Web. 15 Jan 2021.
Vancouver:
Islam MZ. Studying the fluorescence emission properties of 1,2,3 triazine based fluorophores in polymer thin films
. [Internet] [Thesis]. National University of Ireland – Galway; 2017. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10379/6506.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Islam MZ. Studying the fluorescence emission properties of 1,2,3 triazine based fluorophores in polymer thin films
. [Thesis]. National University of Ireland – Galway; 2017. Available from: http://hdl.handle.net/10379/6506
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
16.
Qiu, Xin.
Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores.
Degree: 2013, RIAN
URL: http://eprints.maynoothuniversity.ie/7728/
► As a result of the evolution of antimicrobial resistant bacteria there is a pressing need for novel classes of antibiotics. This project aimed, in part,…
(more)
▼ As a result of the evolution of antimicrobial resistant bacteria there is a pressing need for novel classes of antibiotics. This project aimed, in part, to design and synthesise a new family of pyrazole ligands, their zinc(II) complexes and to evaluate their antmicrobial activity. The overall goal was to improve the antimicrobial activity of both ligand and metal salt through the formation of the corresponding zinc complex. The first pyrazole ligands synthesized were unable to coordinate with zinc(II) salts. We believe this was due to steric hindrance generated by the relatively large pyrazaole ligands. Several smaller pyrazole ligands were synthesized, some of which successfully complexed with zinc suggesting that steric hindrance had indeed been a factor for the first set of ligands. A family of zinc complexes were generated employing the family of smaller pyrazole liagnds. All the pyrazole-zinc complexes, pyrazole ligands and zinc salts were evaluated for activity against S.aureus and E.coli. Generally, the susceptibility test results showed that most pyrazole ligands did not exhibit potent activity against either E. coli or S. aureus. All of the zinc complexes exhibited good antibacterial activity against both E. coli and S. aureus at 100 μg/mL. As we had expected the zinc complexes greatly improved the antibacterial activity of the pyrazole ligands. However, most zinc complexes were as active as the zinc salts alone indicating that the addition of the pyrazole ligands did not improve the activity of the zinc salts.
Organosulfones are widely used in the field of pharmaceuticals and ploymers. Traditional methodologies for synthesizing biphenyl sulfones include Suzuki-Miyaura coupling, Friedel-Crafts and other multistep reactions. These methodologies require catalysts, solvents and some require long reaction times. We have developed a novel solvent-free methodology for synthesizing fluorescent biphenyl sulfones with a relatively short reaction time and in good yields. This methodology exploits an interesting electrocyclisation of bissulfonyl trienes. A new family of substituted biphenyl sulfones resulted. UV-absorption, emission and excitation spectra were generated for the family of biphenyl sulfones and their photophysical characterisitics studied (e.g. Stokes shift, quantum yield, molar extinction coefficients). The biphenyl sulfones with NO2 substituents did not exhibit fluorescence due to the strong electron withdrawing nature of the NO2 group. Other substituted biphenyl sulfones exhibited highly solvatochromic emissions, probably via twisted intramolecular charge transfer (TICT) states. The biphenyl N,N-dimethyl-4'-(phenylsulfonyl)-[1,1'-biphenyl]-4-amine was found to show a very high quantum yield in toluene and dichloromethane (Φ close to 0.9); large Stokes shifts and high molar extinction coefficient in ethylene glycol (ε > 80 000 M-1cm-1). The HOMO-LUMO gaps of a family biphenyl sulfones were plotted against their Stokes shifts (in chloroform) giving an excellent linear correlation (R2 = 0.9978). These…
Subjects/Keywords: Synthesis; Evaluation; New Antimicrobial Agents; Novel Organic Fluorophores
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Qiu, X. (2013). Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores. (Thesis). RIAN. Retrieved from http://eprints.maynoothuniversity.ie/7728/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Qiu, Xin. “Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores.” 2013. Thesis, RIAN. Accessed January 15, 2021.
http://eprints.maynoothuniversity.ie/7728/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Qiu, Xin. “Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores.” 2013. Web. 15 Jan 2021.
Vancouver:
Qiu X. Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores. [Internet] [Thesis]. RIAN; 2013. [cited 2021 Jan 15].
Available from: http://eprints.maynoothuniversity.ie/7728/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Qiu X. Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores. [Thesis]. RIAN; 2013. Available from: http://eprints.maynoothuniversity.ie/7728/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Oregon
17.
White, Brittany.
The Synthesis of Functionalized Cycloparaphenylenes as Novel Biocompatible Fluorescent Probes and Organic Materials.
Degree: PhD, Department of Chemistry and Biochemistry, 2019, University of Oregon
URL: http://hdl.handle.net/1794/24521
► Conjugated macrocycles have emerged as novel structural motifs that modulate the electronic properties of organic molecules because of their strained and contorted structures. Cycloparaphenylenes, known…
(more)
▼ Conjugated macrocycles have emerged as novel structural motifs that modulate the electronic properties of organic molecules because of their strained and contorted structures. Cycloparaphenylenes, known as nanohoops, are a particularly attractive scaffold for the design of new types of carbon nanomaterials because of their size-selective synthesis, radially oriented π-systems and tunable electronic properties. The development of modular syntheses of nanohoops over the past decade should enable the preparation of substituted derivatives that can be tuned for applications in biology and materials science.
Chapter I provides a brief overview of conjugated macrocycles recently reported in the literature with a discussion of the structural effects that are responsible for the remarkable properties of this class of molecules. Chapter II highlights a scalable and mild synthetic approach developed in our lab to prepare nanohoop conjugated macrocycles and expands the generality of this methodology with the formal synthesis of natural product Acerogenin E. Chapter III describes the synthesis of cycloparaphenylenes with versatile functional handles and uncovers the reactivity of the strain nanohoop backbone under reaction conditions that promote the formation of radical cations. Chapter IV takes advantage of the functional groups described in chapter III to develop the first example of nanohoops as a new class of biocompatible
fluorophores. Chapter V details a novel synthetic approach that enables the incorporation of the linear acene pentacene into the nanohoop backbone and reports our findings on the impact that the macrocyclic structure has on the properties of this organic semiconductor. In summary, the findings discussed in this dissertation provide synthetic strategies for the selective functionalization of nanohoops and highlight this class of molecules as a novel scaffold for the design of new types of carbon nanomaterials.
This dissertation includes previously published and unpublished co-authored material.
Advisors/Committee Members: Jasti, Ramesh (advisor).
Subjects/Keywords: Biocompatible fluorophores; Nanohoops; Organic materials; Physical organic chemistry; Synthetic chemistry
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
White, B. (2019). The Synthesis of Functionalized Cycloparaphenylenes as Novel Biocompatible Fluorescent Probes and Organic Materials. (Doctoral Dissertation). University of Oregon. Retrieved from http://hdl.handle.net/1794/24521
Chicago Manual of Style (16th Edition):
White, Brittany. “The Synthesis of Functionalized Cycloparaphenylenes as Novel Biocompatible Fluorescent Probes and Organic Materials.” 2019. Doctoral Dissertation, University of Oregon. Accessed January 15, 2021.
http://hdl.handle.net/1794/24521.
MLA Handbook (7th Edition):
White, Brittany. “The Synthesis of Functionalized Cycloparaphenylenes as Novel Biocompatible Fluorescent Probes and Organic Materials.” 2019. Web. 15 Jan 2021.
Vancouver:
White B. The Synthesis of Functionalized Cycloparaphenylenes as Novel Biocompatible Fluorescent Probes and Organic Materials. [Internet] [Doctoral dissertation]. University of Oregon; 2019. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1794/24521.
Council of Science Editors:
White B. The Synthesis of Functionalized Cycloparaphenylenes as Novel Biocompatible Fluorescent Probes and Organic Materials. [Doctoral Dissertation]. University of Oregon; 2019. Available from: http://hdl.handle.net/1794/24521
University of New Mexico
18.
Deng, Yurong.
Characterization of dissolved organic matter by separation and fluorescence spectroscopy.
Degree: Department of Chemistry and Chemical Biology, 2009, University of New Mexico
URL: https://digitalrepository.unm.edu/chem_etds/3
► The goal of this work is to evaluate new methods of extracting and fractionating dissolved organic matter (DOM) using liquid-liquid and solid-phase extraction (SPE), monitored…
(more)
▼ The goal of this work is to evaluate new methods of extracting and fractionating dissolved organic matter (DOM) using liquid-liquid and solid-phase extraction (SPE), monitored by optical spectroscopy. DOM in aquatic systems from different sources was characterized by three-dimension excitation-emission matrix fluorescence spectroscopy (3DEEMS) and UV absorbance spectroscopy. UV and visible humic-like fluorescence were observed in all water samples – humic acids (HA), fulvic acids (FA), river water, wastewater, and their fractionations. Their fluorescence centers varied with environment in the range of λex/ λem = 220-250 nm/390-460 nm for UV humic-like fluorescence (peak A) and λex/ λem = 300-340 nm/390-460 nm for visible humic-like fluorescence (peak C). pH change didnt shift maximum excitation and emission wavelengths but did change the emission intensities of peaks A and C. Peaks A and C always occur together, although relative intensities may change. Protein-like fluorescence peaks were observed in pairs with higher emission intensity at shorter wavelengths than at longer wavelengths. Tryptophan-like fluorescence (peaks T1 and T2) was observed at λex/ λem = 230 nm/356 nm and λex/ λem = 280 nm/356 nm in river water and wastewater. Tyrosine-like fluorescence (peaks S1 and S2) was observed at λex/ λem = 220 nm/309 nm and λex/ λem = 280 nm/309 nm only in a wastewater sample without extensive biological pretreatment. A new peak was observed at λex/ λem = 250-260 nm/460 nm (peak B), and overlapped with peaks A and C in all water samples and their isolates. Another peak specific to one river water sample was observed at λex/ λem =260 nm/340 (peak D) which could be mis-identified as peak T2. Partitioning of NOM into organic solvents was investigated with and without ion-pairing reagent. No extraction of either peak A or C occurred without ion-pairing reagent. Alteration of the partitioning of these two
fluorophores by ion-pairing reagent and non-polar solvents enriched peak A in the aqueous phase and peak C in the organic phase. Maximum excitation and emission wavelengths shifted with the addition of ion-pairing reagent due to enhanced peak overlapping and solvent effects. Peak A is the sum of several superposed peaks rather than a simple one. Liquid-liquid extraction could separate different
fluorophores but it's not easy to use. Rio Grande river water was isolated and fractionated using Solid-phase Extraction (SPE). Humic-like
fluorophores (peaks A and C) could be retained by and eluted from the apolar Sep-pak C18 cartridge, Empore C18 Disk, polymeric Oasis HLB and MAX cartridges. Both humic-like
fluorophores are negatively charged and visible humic-like
fluorophores (peak C) are more hydrophobic than UV humic-like
fluorophores (peak A). Tryptophan-like
fluorophores were excluded from those sorbents and occur as neutral or positively charged hydrophilic molecules. Based on the extraction recovery, Empore C18 Disk has the highest recovery (90%) for humic-like
fluorophores and Oasis HLB is good for isolating…
Advisors/Committee Members: Cabaniss, stephen, Howe, Kerry, Keller, David, Wang, wei.
Subjects/Keywords: Dissolved organic matter; fluorophores; separation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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Manager
APA (6th Edition):
Deng, Y. (2009). Characterization of dissolved organic matter by separation and fluorescence spectroscopy. (Doctoral Dissertation). University of New Mexico. Retrieved from https://digitalrepository.unm.edu/chem_etds/3
Chicago Manual of Style (16th Edition):
Deng, Yurong. “Characterization of dissolved organic matter by separation and fluorescence spectroscopy.” 2009. Doctoral Dissertation, University of New Mexico. Accessed January 15, 2021.
https://digitalrepository.unm.edu/chem_etds/3.
MLA Handbook (7th Edition):
Deng, Yurong. “Characterization of dissolved organic matter by separation and fluorescence spectroscopy.” 2009. Web. 15 Jan 2021.
Vancouver:
Deng Y. Characterization of dissolved organic matter by separation and fluorescence spectroscopy. [Internet] [Doctoral dissertation]. University of New Mexico; 2009. [cited 2021 Jan 15].
Available from: https://digitalrepository.unm.edu/chem_etds/3.
Council of Science Editors:
Deng Y. Characterization of dissolved organic matter by separation and fluorescence spectroscopy. [Doctoral Dissertation]. University of New Mexico; 2009. Available from: https://digitalrepository.unm.edu/chem_etds/3
19.
Qiu, Xin.
Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores.
Degree: 2013, RIAN
URL: http://mural.maynoothuniversity.ie/7728/
► As a result of the evolution of antimicrobial resistant bacteria there is a pressing need for novel classes of antibiotics. This project aimed, in part,…
(more)
▼ As a result of the evolution of antimicrobial resistant bacteria there is a pressing need for novel classes of antibiotics. This project aimed, in part, to design and synthesise a new family of pyrazole ligands, their zinc(II) complexes and to evaluate their antmicrobial activity. The overall goal was to improve the antimicrobial activity of both ligand and metal salt through the formation of the corresponding zinc complex. The first pyrazole ligands synthesized were unable to coordinate with zinc(II) salts. We believe this was due to steric hindrance generated by the relatively large pyrazaole ligands. Several smaller pyrazole ligands were synthesized, some of which successfully complexed with zinc suggesting that steric hindrance had indeed been a factor for the first set of ligands. A family of zinc complexes were generated employing the family of smaller pyrazole liagnds. All the pyrazole-zinc complexes, pyrazole ligands and zinc salts were evaluated for activity against S.aureus and E.coli. Generally, the susceptibility test results showed that most pyrazole ligands did not exhibit potent activity against either E. coli or S. aureus. All of the zinc complexes exhibited good antibacterial activity against both E. coli and S. aureus at 100 μg/mL. As we had expected the zinc complexes greatly improved the antibacterial activity of the pyrazole ligands. However, most zinc complexes were as active as the zinc salts alone indicating that the addition of the pyrazole ligands did not improve the activity of the zinc salts.
Organosulfones are widely used in the field of pharmaceuticals and ploymers. Traditional methodologies for synthesizing biphenyl sulfones include Suzuki-Miyaura coupling, Friedel-Crafts and other multistep reactions. These methodologies require catalysts, solvents and some require long reaction times. We have developed a novel solvent-free methodology for synthesizing fluorescent biphenyl sulfones with a relatively short reaction time and in good yields. This methodology exploits an interesting electrocyclisation of bissulfonyl trienes. A new family of substituted biphenyl sulfones resulted. UV-absorption, emission and excitation spectra were generated for the family of biphenyl sulfones and their photophysical characterisitics studied (e.g. Stokes shift, quantum yield, molar extinction coefficients). The biphenyl sulfones with NO2 substituents did not exhibit fluorescence due to the strong electron withdrawing nature of the NO2 group. Other substituted biphenyl sulfones exhibited highly solvatochromic emissions, probably via twisted intramolecular charge transfer (TICT) states. The biphenyl N,N-dimethyl-4'-(phenylsulfonyl)-[1,1'-biphenyl]-4-amine was found to show a very high quantum yield in toluene and dichloromethane (Φ close to 0.9); large Stokes shifts and high molar extinction coefficient in ethylene glycol (ε > 80 000 M-1cm-1). The HOMO-LUMO gaps of a family biphenyl sulfones were plotted against their Stokes shifts (in chloroform) giving an excellent linear correlation (R2 = 0.9978). These…
Subjects/Keywords: Synthesis; Evaluation; New Antimicrobial Agents; Novel Organic Fluorophores
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Record Details
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Qiu, X. (2013). Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores. (Thesis). RIAN. Retrieved from http://mural.maynoothuniversity.ie/7728/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Qiu, Xin. “Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores.” 2013. Thesis, RIAN. Accessed January 15, 2021.
http://mural.maynoothuniversity.ie/7728/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Qiu, Xin. “Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores.” 2013. Web. 15 Jan 2021.
Vancouver:
Qiu X. Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores. [Internet] [Thesis]. RIAN; 2013. [cited 2021 Jan 15].
Available from: http://mural.maynoothuniversity.ie/7728/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Qiu X. Synthesis and Evaluation of New Antimicrobial Agents and Novel Organic Fluorophores. [Thesis]. RIAN; 2013. Available from: http://mural.maynoothuniversity.ie/7728/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Georgia Tech
20.
Jablonski, Amy E.
Optically modulated fluorescent proteins.
Degree: PhD, Chemistry and Biochemistry, 2014, Georgia Tech
URL: http://hdl.handle.net/1853/52327
► Optical modulation has shown the selective and sensitive signal improvement in high background systems in cell imaging; however, cell applications are still limited due to…
(more)
▼ Optical modulation has shown the selective and sensitive signal improvement in high background systems in cell imaging; however, cell applications are still limited due to biocompatibility and delivery issues. Fluorescent proteins have a variety of optically accessible states that make them ideal candidates for investigation of modulatability. Combining the optical modulation technique with the biocompatibility of fluorescent proteins is a major advance. This work focuses on evaluation fluorescent proteins and their optical states for modulation, as well demonstrations of cellular imaging. Herein, we evaluate a green fluorescent protein with interesting photophysical properties favorable for optical modulation. Positive for optical modulation, further investigation of the state dictating modulation reveals the presence of a slow component on the order of milliseconds. To better understand the mechanism responsible modulation, blue fluorescent proteins are created to modify the chromophore environment. Extraction of photophysics confirm the alteration timescales of the modulated state. Motivated by the ability to improve imaging and decode hidden dynamics, demodulation of these proteins demonstrates the selective recovery of signal in the presence of high cellular background. The continued investigation of several other fluorescent proteins identifies modulatable proteins across the visible wavelength region. Additionally, solvent environmental factors show varying timescales which, when combined with mutagenesis, suggest a cis/trans isomerization coupled with a proton transfer. This information of the properties dictating optical modulation allows for the engineering of improved modulatable proteins to study cellular dynamics.
Advisors/Committee Members: Dickson, Robert M. (advisor), Tolbert, Laren M. (committee member), Fahrni, Christoph (committee member), El-Sayed, Mostafa A. (committee member), Kemp, Melissa (committee member).
Subjects/Keywords: Fluorescent proteins; Protein dynamics; Microscopy; Signal recovery; Chromophore; Fluorophores
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APA ·
Chicago ·
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Export
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APA (6th Edition):
Jablonski, A. E. (2014). Optically modulated fluorescent proteins. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/52327
Chicago Manual of Style (16th Edition):
Jablonski, Amy E. “Optically modulated fluorescent proteins.” 2014. Doctoral Dissertation, Georgia Tech. Accessed January 15, 2021.
http://hdl.handle.net/1853/52327.
MLA Handbook (7th Edition):
Jablonski, Amy E. “Optically modulated fluorescent proteins.” 2014. Web. 15 Jan 2021.
Vancouver:
Jablonski AE. Optically modulated fluorescent proteins. [Internet] [Doctoral dissertation]. Georgia Tech; 2014. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1853/52327.
Council of Science Editors:
Jablonski AE. Optically modulated fluorescent proteins. [Doctoral Dissertation]. Georgia Tech; 2014. Available from: http://hdl.handle.net/1853/52327
Louisiana State University
21.
Uppal, Timsy Kaur.
Synthesis and Characterization of Red and Near-Infrared BODIPY-based fluorophores for various Biological Applications.
Degree: PhD, Chemistry, 2012, Louisiana State University
URL: etd-07062012-131952
;
https://digitalcommons.lsu.edu/gradschool_dissertations/631
► ABSTRACT 4,4-Difluoro-3a,4a-diaza-s-indacene, better known as BODIPY® dyes, have attracted considerable attention due to their intriguing physicochemical and spectral properties, including high absorption coefficient, fluorescence quantum…
(more)
▼ ABSTRACT 4,4-Difluoro-3a,4a-diaza-s-indacene, better known as BODIPY® dyes, have attracted considerable attention due to their intriguing physicochemical and spectral properties, including high absorption coefficient, fluorescence quantum yield and good photochemical stability. Despite the recent progress achieved in this field, the synthesis of BODIPY derivatives that absorb and emit in the red- and NIR-region of the electromagnetic spectrum and their application to biomolecular targets, represents a long-standing challenge in BODIPY chemistry. This research work is therefore focused on the synthesis and characterization of red and NIR-emissive BODIPY-based fluorophores for various bioanalytical and biomedical applications. Chapter 1 of this dissertation represents a concise introduction to the fundamental concepts, synthetic routes, post-synthetic modification strategies and various biological applications of BODIPY dyes, that are further elaborated upon in the following Chapters. Chapter 2 describes the synthesis, characterization, computational modeling and cellular investigations of a series of new functionalized mono- and dibenzo-appended BODIPYs that are promising fluorophores for biolabeling applications. These ring expanded and constrained BODIPYs were synthesized via two different routes from a common tetrahydroisoindole precursor. This work was done in collaboration with Dr. Petia Bobadova-Parvanova of Rockhurst University. Chapter 3 reports on the synthesis and characterization of several styryl- and lysyl-BODIPY conjugates, prepared using Knoevenagel condensation reaction, an attractive strategy for the expeditious synthesis of π-extended BODIPYs in moderate to high yields. The effects of the styryl substituents on their photophysical properties and in vitro photodynamic activities are also described. Chapter 4 discusses an efficient method towards the preparation of a series of BODIPY-PEG and BODIPY-Carbohydrate conjugates via Cu(I)-mediated azide/alkyne cycloaddition, i.e. “click” chemistry. Several NIR-emissive BODIPY conjugates that are potential in vivo imaging agents were prepared in good to excellent overall yields. The photophysical studies of novel BODIPY-PEG and BODIPY-Carbohydrate conjugates are described in addition to the preliminary in vitro investigations.
Subjects/Keywords: BODIPY Dyes; Fluorophores; Near-Infrared; Photophysical properties; Cellular studies
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APA (6th Edition):
Uppal, T. K. (2012). Synthesis and Characterization of Red and Near-Infrared BODIPY-based fluorophores for various Biological Applications. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07062012-131952 ; https://digitalcommons.lsu.edu/gradschool_dissertations/631
Chicago Manual of Style (16th Edition):
Uppal, Timsy Kaur. “Synthesis and Characterization of Red and Near-Infrared BODIPY-based fluorophores for various Biological Applications.” 2012. Doctoral Dissertation, Louisiana State University. Accessed January 15, 2021.
etd-07062012-131952 ; https://digitalcommons.lsu.edu/gradschool_dissertations/631.
MLA Handbook (7th Edition):
Uppal, Timsy Kaur. “Synthesis and Characterization of Red and Near-Infrared BODIPY-based fluorophores for various Biological Applications.” 2012. Web. 15 Jan 2021.
Vancouver:
Uppal TK. Synthesis and Characterization of Red and Near-Infrared BODIPY-based fluorophores for various Biological Applications. [Internet] [Doctoral dissertation]. Louisiana State University; 2012. [cited 2021 Jan 15].
Available from: etd-07062012-131952 ; https://digitalcommons.lsu.edu/gradschool_dissertations/631.
Council of Science Editors:
Uppal TK. Synthesis and Characterization of Red and Near-Infrared BODIPY-based fluorophores for various Biological Applications. [Doctoral Dissertation]. Louisiana State University; 2012. Available from: etd-07062012-131952 ; https://digitalcommons.lsu.edu/gradschool_dissertations/631
Macquarie University
22.
Howden, Michael George.
Synthesis of highly fluorescent tuneable boron complexes.
Degree: 2016, Macquarie University
URL: http://hdl.handle.net/1959.14/1265231
► Empirical thesis.
Chapter One. Introduction – Chapter Two.4,5-Di(2’-pyridyl)imidazoles (BODPIs) – Chapter Three. Extended π-Conjugated 4,5-Di(2’-pyridyl)imidazoles (BODPIs) – Chapter Four. 2-(2’-Pyridyl)pyrroles (BOPYPYs) – Chapter Five. Pyridyl-Benzimidazoles…
(more)
▼ Empirical thesis.
Chapter One. Introduction – Chapter Two.4,5-Di(2’-pyridyl)imidazoles (BODPIs) – Chapter Three. Extended π-Conjugated 4,5-Di(2’-pyridyl)imidazoles (BODPIs) – Chapter Four. 2-(2’-Pyridyl)pyrroles (BOPYPYs) – Chapter Five. Pyridyl-Benzimidazoles (BOPYBIs) – Chapter Six. Future work.
The boron-dipyrromethene (BODIPY) dyes are without doubt the most well-known of the fluorescent boron complexes to date. With vast amounts of success seen with BODIPYs, researchinto the synthesis of novel families of heterocyclic ligands that are capable of chelating boron to produce highly fluorescent compounds is an area of expanding interest.
This Thesis describes the design, synthesis of three families of heterocyclic boron complexes and characterisation of their photophysical properties. The families include boron 4,5-di(2’-pyridyl)imidazole difluoride (BODPIs), boron 2-(2’-pyridyl)pyrroles difluoride (BOPYPYs) and boron 2-(2’-pyridyl)benzimidazole diphenyl (BOPYBIs). Chapter Two highlights the synthesis of a new diverse family of boron difluoride 4,5-di(2’-pyridyl)imidazole (BODPI) dyes via a Debus-Radziszewski like condensation of 2,2’-pyridil with substituted benzaldehydes. These BODPIs exhibited excellent dye properties including large Stokes shifts, strong fluorescence in solution, solid state fluorescence, solvatochromism and a tuneable emission profile.
In Chapter Three, BODPI derivatives with increased π-conjugation were prepared using two strategies and their photophysical properties were studied. The first strategy used was reacting several commercially available fused aryl aldehydes and the second was the use 2-(4”-formylphenyl)-4,5-di(2’-pyridyl)imidazole in the Knoevenagel condensation with active methylene substrates.
In Chapter Four, the effect of substituents on the BOPYPY framework and their photophysical properties were studied. Symmetric, asymmetric and styryl BOPYPY exhibited strong fluorescence in both solution and solid state, solvatochromism, large Stokes shifts and are readily tuneable by the choice of the starting 1,3-diketone derivative.
Chapter Five describes the preliminary study of BOPYBIs and their photophysical properties .Unlike other families described in this Thesis, BOPYBIs formed a boron complex using triphenylborane in place of boron trifluoride. The chemical and photophysical properties described in this chapter demonstrate the potential practicality of these compounds in applications such as fluorescent dyes and/or OLEDs.
1 online resource (xiv, 275 pages) colour illustrations
Advisors/Committee Members: Macquarie University. Department of Molecular Sciences.
Subjects/Keywords: Boron compounds – Synthesis; Fluorescence; fluorescence; boron; fluorophores; organic
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APA (6th Edition):
Howden, M. G. (2016). Synthesis of highly fluorescent tuneable boron complexes. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/1265231
Chicago Manual of Style (16th Edition):
Howden, Michael George. “Synthesis of highly fluorescent tuneable boron complexes.” 2016. Doctoral Dissertation, Macquarie University. Accessed January 15, 2021.
http://hdl.handle.net/1959.14/1265231.
MLA Handbook (7th Edition):
Howden, Michael George. “Synthesis of highly fluorescent tuneable boron complexes.” 2016. Web. 15 Jan 2021.
Vancouver:
Howden MG. Synthesis of highly fluorescent tuneable boron complexes. [Internet] [Doctoral dissertation]. Macquarie University; 2016. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/1959.14/1265231.
Council of Science Editors:
Howden MG. Synthesis of highly fluorescent tuneable boron complexes. [Doctoral Dissertation]. Macquarie University; 2016. Available from: http://hdl.handle.net/1959.14/1265231
University of Akron
23.
Tamgho, Ingrid-Suzy.
Synthesis of Ligands and Macrocycles Based on
1,3-Diiminoisoindoline and Study of New Highly Fluorescent and
Symmetric Pyrrole-BF2 Chromophores.
Degree: PhD, Chemistry, 2014, University of Akron
URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1412163224
► Discovered in 1907 by Braun and Tcherniac, a phthalocyanine is a macrocycle composed of four isoindolines. The metal chemistry of this macrocycle is rich, as…
(more)
▼ Discovered in 1907 by Braun and Tcherniac, a
phthalocyanine is a macrocycle composed of four isoindolines. The
metal chemistry of this macrocycle is rich, as of today, the
phthalocyanines are well-known dyes and photosensitizers. Among the
variety of phthalocyanine precursors, 1,3-diiminoisoindoline and
phthalonitrile are the most widely used. Indeed, new macrocyles and
ligands are synthesized from these compounds, either by
condensation of 1,3-diiminoisoindoline or the
CaCl
2-catalyzed reaction of phthalonitrile
with an amine. Following these methods, we are able to prepare a
series of compounds, the phthalocrown, and also revisit the
chemistry of bis(alkylimino)isoindolines. The first part of the
dissertation describes the synthesis and characterization of
phthalocrowns. They can be considered as chimera molecules composed
of a crown ether and phthalocyanine. These compounds are
synthesized via Schiff base-type condensation reactions between
polyetherdiamines and 1,3-diiminoisoindoline, which can be
generated from phthalonitrile. Secondly, we revisit the chemistry
of bis(alkylimino)isoindolines. These compounds can be generated
via phthalonitrile and 1,3-diiminoisoindoline depending on the
nature of the amine. Also, the metal chemistry of these compounds
with Ag(I) is described. One important class of fluorescent dyes is
known as BODIPY. This fluorophore consists of a dipyrromethene
bound to a central BF
2 unit. The compound is
found to possess excellent photophysical properties such as high
extinction coefficient and high quantum yield of emission.
Moreover, BODIPY is insensitive to pH and solvent polarity and is
photostable. Its versatility, introduced via the attachment of
biomolecules or other moieties, allows these dyes to have important
applications from biological imaging and sensing to light
harvesting. In the final chapter, we discuss the synthesis of a
novel family of
fluorophores, the BOPHY. These dyes are synthesized
via the Schiff-base reaction of a pyrrole carbaldehyde with
hydrazine, followed with a BF
2 complexation
to yield highly fluorescent chromophores. The BOPHY optical
properties are discussed in the final chapter as
well.
Advisors/Committee Members: Ziegler, Christopher (Advisor).
Subjects/Keywords: Chemistry; phthalocyanine, DII, schiff bases, bodipy, bophy,
fluorophores, isoindolines, N-heterocycle
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APA (6th Edition):
Tamgho, I. (2014). Synthesis of Ligands and Macrocycles Based on
1,3-Diiminoisoindoline and Study of New Highly Fluorescent and
Symmetric Pyrrole-BF2 Chromophores. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1412163224
Chicago Manual of Style (16th Edition):
Tamgho, Ingrid-Suzy. “Synthesis of Ligands and Macrocycles Based on
1,3-Diiminoisoindoline and Study of New Highly Fluorescent and
Symmetric Pyrrole-BF2 Chromophores.” 2014. Doctoral Dissertation, University of Akron. Accessed January 15, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=akron1412163224.
MLA Handbook (7th Edition):
Tamgho, Ingrid-Suzy. “Synthesis of Ligands and Macrocycles Based on
1,3-Diiminoisoindoline and Study of New Highly Fluorescent and
Symmetric Pyrrole-BF2 Chromophores.” 2014. Web. 15 Jan 2021.
Vancouver:
Tamgho I. Synthesis of Ligands and Macrocycles Based on
1,3-Diiminoisoindoline and Study of New Highly Fluorescent and
Symmetric Pyrrole-BF2 Chromophores. [Internet] [Doctoral dissertation]. University of Akron; 2014. [cited 2021 Jan 15].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1412163224.
Council of Science Editors:
Tamgho I. Synthesis of Ligands and Macrocycles Based on
1,3-Diiminoisoindoline and Study of New Highly Fluorescent and
Symmetric Pyrrole-BF2 Chromophores. [Doctoral Dissertation]. University of Akron; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1412163224
24.
Dang, Florian-Xuan.
Syntheses and applications of functional dyes based on styrylpyrylium and styrylpyridinium salts : Synthesis of hydrosoluble cryptophanes, designed to encapsulate xenon, and of hemicryptophanes functionnalized to complex lanthanide (III) ions, with the intention to apply them in medical imaging.
Degree: Docteur es, Chimie organique, 2015, Aix-Marseille; Institut des sciences moléculaires de Marseille (ISM2, UMR 7313)
URL: http://www.theses.fr/2015AIXM4372
► Les travaux effectués durant cette thèse ont eu pour objectif le développement de chromophores fonctionnels à base de sels de styrylpyrylium et styrylpyridinium. Les divers…
(more)
▼ Les travaux effectués durant cette thèse ont eu pour objectif le développement de chromophores fonctionnels à base de sels de styrylpyrylium et styrylpyridinium. Les divers composés synthétisés ont montré une très grande flexibilité concernant leurs propriétés optiques, avec notamment des longueurs d’onde d’absorption et d’émission couvrant la quasi-totalité du spectre visible. Associée à la variabilité structurelle inhérente à ce type de chromophore, il est possible d’obtenir des composés aux propriétés modulables, et intégrables dans une large gamme d’applications.Ce manuscrit est constitué de trois parties principales. La première décrit la synthèse et les propriétés photophysiques de divers chromophores obtenus durant cette thèse. La seconde décrit l’approche théorique, utilisée pour faciliter la conception et l’analyse des composés étudiés. Finalement, la troisième partie décrit les applications pour lesquelles certaines variations de sels de styrylpyrylium et styrylpyridinium ont étés spécialement développés.
The work done during this thesis aimed to develop functional chromophores based on styrylpyrylium and styrylpyrylium salts. The compounds synthesized have shown a great flexibility regarding their optical properties, including maximum wavelength of absorption and emission covering almost the entire visible spectrum. Combined to their structural adaptability, it was possible to design various compounds compatible with a wide range of applications.This manuscript is composed of three main parts. The first part describes the synthesis and the photophysical properties of some chromophores obtained during this thesis. The second describes the theoretical approach, used to assist the design and the analysis of the studied compounds. Finally, the third part describes various applications for which some styrylpyrylium and styrylpyridinium salts have been specially designed.
Advisors/Committee Members: Balaban, Teodor Silviu (thesis director).
Subjects/Keywords: Styrylpyrylium; Styrylpyridinium; Synthèse; Chromophores Fonctionnels; Fluorophores; Dft; Td-Dft; Fonctionnalisation de Surfaces; Fonctionnalisation de Peptides; Styrylpyrylium; Styrylpyridinium; Synthesis; Functional Dyes; Fluorophores; Dft; Td-Dft; Surface Functionalization; Peptide Functionalization
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APA (6th Edition):
Dang, F. (2015). Syntheses and applications of functional dyes based on styrylpyrylium and styrylpyridinium salts : Synthesis of hydrosoluble cryptophanes, designed to encapsulate xenon, and of hemicryptophanes functionnalized to complex lanthanide (III) ions, with the intention to apply them in medical imaging. (Doctoral Dissertation). Aix-Marseille; Institut des sciences moléculaires de Marseille (ISM2, UMR 7313). Retrieved from http://www.theses.fr/2015AIXM4372
Chicago Manual of Style (16th Edition):
Dang, Florian-Xuan. “Syntheses and applications of functional dyes based on styrylpyrylium and styrylpyridinium salts : Synthesis of hydrosoluble cryptophanes, designed to encapsulate xenon, and of hemicryptophanes functionnalized to complex lanthanide (III) ions, with the intention to apply them in medical imaging.” 2015. Doctoral Dissertation, Aix-Marseille; Institut des sciences moléculaires de Marseille (ISM2, UMR 7313). Accessed January 15, 2021.
http://www.theses.fr/2015AIXM4372.
MLA Handbook (7th Edition):
Dang, Florian-Xuan. “Syntheses and applications of functional dyes based on styrylpyrylium and styrylpyridinium salts : Synthesis of hydrosoluble cryptophanes, designed to encapsulate xenon, and of hemicryptophanes functionnalized to complex lanthanide (III) ions, with the intention to apply them in medical imaging.” 2015. Web. 15 Jan 2021.
Vancouver:
Dang F. Syntheses and applications of functional dyes based on styrylpyrylium and styrylpyridinium salts : Synthesis of hydrosoluble cryptophanes, designed to encapsulate xenon, and of hemicryptophanes functionnalized to complex lanthanide (III) ions, with the intention to apply them in medical imaging. [Internet] [Doctoral dissertation]. Aix-Marseille; Institut des sciences moléculaires de Marseille (ISM2, UMR 7313); 2015. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2015AIXM4372.
Council of Science Editors:
Dang F. Syntheses and applications of functional dyes based on styrylpyrylium and styrylpyridinium salts : Synthesis of hydrosoluble cryptophanes, designed to encapsulate xenon, and of hemicryptophanes functionnalized to complex lanthanide (III) ions, with the intention to apply them in medical imaging. [Doctoral Dissertation]. Aix-Marseille; Institut des sciences moléculaires de Marseille (ISM2, UMR 7313); 2015. Available from: http://www.theses.fr/2015AIXM4372
25.
Fang, Zhizhou.
Development of a conformation
sensitive assay for the detection of inter-domain interactions
mediated by allosteric akt inhibitors.
Degree: 2013, Technische Universität Dortmund
URL: http://hdl.handle.net/2003/30564
► Die Aktivität vieler Proteinkinasen wird durch das Zusammenspiel der Kinasedomäne mit zusätzlichen regulatorischen Domänen gesteuert. Daher besitzen niedermolekulare Verbindungen, die solche Interdomänen-Interaktionen allosterisch adressieren und…
(more)
▼ Die Aktivität vieler
Proteinkinasen wird durch das Zusammenspiel der Kinasedomäne mit
zusätzlichen regulatorischen Domänen gesteuert. Daher besitzen
niedermolekulare Verbindungen, die solche
Interdomänen-Interaktionen allosterisch adressieren und modulieren
können, einen großen Wert in der chemischen Biologie und der
Pharmaforschung, da diese Interaktionen oft spezifisch für
bestimmte Kinasen bzw. Kinasefamilien sind, und somit im Gegensatz
zu klassischen ATP-kompetitiven Inhibitoren eine höhere
Selektivität versprechen. Die Proteinkinase B (PKB, auch genannt
Akt) ist eine solche Multidomänen-Kinase und stellt ein wichtiges
Zielprotein in der Krebsforschung dar. Im Hochdurchsatzscreening
wurden in den letzten Jahren eine neue Familie von allosterischen
Akt Inhibitoren entdeckt, deren Wirkmechanismus die Anwesenheit
einer regulatorischen PH Domäne voraussetzt, da sie in einer
Bindungstasche an der Grenzfläche zwischen der Kinase- und PH
Domäne binden und dadurch Akt in einer inaktiven Konformation
arretieren. Diese einzigartige Bindetasche führt zu einer
außergewöhnlich hohen Selektivität und somit in vivo potenziell
niedrigen Toxizität durch Reduktion unerwünschter
Arzneimittelwirkungen. Allerdings basieren alle bisher bekannten
Interdomänen-Inhibitoren auf demselben chemischen Grundgerüst, und
die Identifizierung von neuartigen Leitstrukturen wird durch den
Mangel an geeigneten Hochdurchsatzmethoden ausgebremst. In der
vorliegenden Arbeit wird die Entwicklung von iFLiK (interface-FLiK)
beschrieben, einem Fluoreszenz-basierten Assay, der solche
Interdomänen-Interaktionen detektieren kann. Die für iFLiK
benötigten Proteine wurden anhand kristallographischer
Informationen konzipiert, in Insektenzellen exprimiert und
schließlich mit verschiedenen Fluorophoren gekuppelt, um ihre
Eignung als Sensoren für Proteinkonformationen sys-tematisch zu
untersuchen. Zur Etablierung des Assays wurden zudem eine Reihe von
Sondenmolekülen synthetisiert und auf ihre biologische Aktivität in
vitro und in BT474 Zellen untersucht. Mit iFLiK konnten
allosterische Inhibitoren von ATP-kompetitiven unterschieden und
somit ein Machbarkeitsbeweis erbracht werden, dass solvatochrome
Fluorophore zur Detektion von Protein-Protein-Interaktionen
geeignet sind, wenn der Fluorophor an der korrekten Stelle
angebracht ist. Nach sorgsamer und eingehender Assayoptimierung
sowie Validierung mit orthogonalen Assaysystemen wurde eine interne
Substanzbibliothek mit iFLiK durchmustert. Dadurch konnten
Verbindungen mit einem neuartigen Grundgerüst gefunden werden, die
vorher nicht als allosterische Akt Inhibitoren bekannt waren.
In
addition to the catalytically active kinase domain, many kinases
feature regulatory domains that govern their activity. Modulating
and interfering with these inter-domain interactions presents a
major opportunity for understanding biological systems and
developing innovative therapeutics. Therefore, small molecule
inhibitors that target these interactions with an allosteric mode
of action have great value and higher intrinsic…
Advisors/Committee Members: Rauh, Daniel.
Subjects/Keywords: Akt; Allosteric inhibition;
Environmentally sensitive fluorophores; Inter-domain interactions;
PKB; Protein kinases; 540; 570
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fang, Z. (2013). Development of a conformation
sensitive assay for the detection of inter-domain interactions
mediated by allosteric akt inhibitors. (Thesis). Technische Universität Dortmund. Retrieved from http://hdl.handle.net/2003/30564
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fang, Zhizhou. “Development of a conformation
sensitive assay for the detection of inter-domain interactions
mediated by allosteric akt inhibitors.” 2013. Thesis, Technische Universität Dortmund. Accessed January 15, 2021.
http://hdl.handle.net/2003/30564.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fang, Zhizhou. “Development of a conformation
sensitive assay for the detection of inter-domain interactions
mediated by allosteric akt inhibitors.” 2013. Web. 15 Jan 2021.
Vancouver:
Fang Z. Development of a conformation
sensitive assay for the detection of inter-domain interactions
mediated by allosteric akt inhibitors. [Internet] [Thesis]. Technische Universität Dortmund; 2013. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/2003/30564.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fang Z. Development of a conformation
sensitive assay for the detection of inter-domain interactions
mediated by allosteric akt inhibitors. [Thesis]. Technische Universität Dortmund; 2013. Available from: http://hdl.handle.net/2003/30564
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
McMaster University
26.
Wong, Felix.
pH and Temperature Measurements in Biological Systems Utilizing the Environmental Sensitivity of Proton Transfer in Fluorophores.
Degree: PhD, 2010, McMaster University
URL: http://hdl.handle.net/11375/19199
► A great number of cellular processes can lead to local changes in proton concentration and temperature. So, it is desirable to be able to…
(more)
▼ A great number of cellular processes can lead to local changes in proton
concentration and temperature. So, it is desirable to be able to measure pH and
temperature with non-invasive and spatially resolved methods. In this thesis, I
describe two unique methods to measure pH and temperature using the
environmental sensitivity of the proton transfer of fluorescent molecules. The first
method is based on the detection of the temperature and pH dependent parameters
associated with the blinking of fluorophores by fluorescence correlation
spectroscopy (FCS). Employing EGFP as the probe, I used this method to
characterize temperature increase at a laser focus due to light absorption in a thin
liquid sample. Using pyranine as a probe, I extended the applicability of this
method to a range of pH including physiological pH. Also, I investigated in
details the effect of buffer composition on the blinking of the fluorophores. Then,
I concluded that one limitation of this method is its strong dependence on buffer
conditions, which are not well characterized in vivo. The second approach is a pH
measurement method based on ratiometric imaging. This method is not as
dependent on buffer condition. We improved on current ratiometric imaging
techniques by demonstrating the possibility of using two-photon excitation. This
method was used to measure pH in pyranine loaded vesicles created during
receptor-mediated endocytosis of µ-Opioid receptors expressed in HEK 293 cells.
Preliminary results showed that the pH in the endocytic vesicles dropped to a
value similar to those measurements in late endosomes roughly~ 10 minutes after
triggering the endocytosis, and eventually, the pH reading reached a value similar
to that of the pH oflysosomes.
Thesis
Doctor of Philosophy (PhD)
Advisors/Committee Members: Fradin, Cecile, Physics and Astronomy.
Subjects/Keywords: temperature; biological system; proton transfer; Fluorophores
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APA ·
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MLA ·
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Export
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Manager
APA (6th Edition):
Wong, F. (2010). pH and Temperature Measurements in Biological Systems Utilizing the Environmental Sensitivity of Proton Transfer in Fluorophores. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/19199
Chicago Manual of Style (16th Edition):
Wong, Felix. “pH and Temperature Measurements in Biological Systems Utilizing the Environmental Sensitivity of Proton Transfer in Fluorophores.” 2010. Doctoral Dissertation, McMaster University. Accessed January 15, 2021.
http://hdl.handle.net/11375/19199.
MLA Handbook (7th Edition):
Wong, Felix. “pH and Temperature Measurements in Biological Systems Utilizing the Environmental Sensitivity of Proton Transfer in Fluorophores.” 2010. Web. 15 Jan 2021.
Vancouver:
Wong F. pH and Temperature Measurements in Biological Systems Utilizing the Environmental Sensitivity of Proton Transfer in Fluorophores. [Internet] [Doctoral dissertation]. McMaster University; 2010. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/11375/19199.
Council of Science Editors:
Wong F. pH and Temperature Measurements in Biological Systems Utilizing the Environmental Sensitivity of Proton Transfer in Fluorophores. [Doctoral Dissertation]. McMaster University; 2010. Available from: http://hdl.handle.net/11375/19199
27.
Fang, Zhizhou.
Development of a conformation sensitive assay for the detection of inter-domain interactions mediated by allosteric akt inhibitors.
Degree: 2013, Technische Universität Dortmund
URL: http://dx.doi.org/10.17877/DE290R-5596
► In addition to the catalytically active kinase domain, many kinases feature regulatory domains that govern their activity. Modulating and interfering with these inter-domain interactions presents…
(more)
▼ In addition to the catalytically active kinase domain, many kinases feature regulatory domains that govern their activity. Modulating and interfering with these inter-domain interactions presents a major opportunity for understanding biological systems and developing innovative therapeutics. Therefore, small molecule inhibitors that target these interactions with an allosteric mode of action have great value and higher intrinsic selectivity, as these interactions are often unique for a single kinase or kinase family. Protein Kinase B (PKB, also known as Akt) is such a multi-domain kinase and represents an important target in cancer research. A number of allosteric inhibitors have been reported for Akt over the last years, with one of them currently in clinical phase II trials. Their mode of action requires the presence of a regulatory PH domain as they bind at the PH/kinase domain interface, locking Akt into an inactive conformation. This unique
binding site enables an extraordinary high selectivity and therefore low off-target toxicity. However, all known inter-domain inhibitors so far are based on the same scaffold, and the discovery of new chemical entities is hampered by the lack of methods to identify such inhibitors in high-throughput screens. This thesis describes the development of iFLiK (interface-FLiK), a fluorescence-based assay that can monitor such inter-domain interactions. Guided by X-ray crystal structures, the assay was designed to sense the conformational changes of the protein associated with these interactions by attaching an environmentally sensitive fluorophore to a suitable site. In order to establish the assay, a series of probe molecules were synthesised and tested for their inhibitory activity in in vitro and cellular assays. Furthermore, the proteins required for iFLiK were expressed in insect cells and labelled with various
fluorophores, systematically assessing their suitability to report on
protein conformations. iFLiK could easily distinguish allosteric from classic ATP-competitive inhibitors, thereby providing a proof of principle that solvatochromic
fluorophores are capable to monitor protein-protein interactions when attaching the sensor to the correct position. After extensive and careful assay optimisation and validation with orthogonal assay systems, an in-house library was screened with iFLiK. Novel compounds with a scaffold that was previously not described as allosteric Akt inhibitors were identified.
Advisors/Committee Members: Rauh, Daniel (advisor), Waldmann, Herbert (referee).
Subjects/Keywords: Akt; PKB; Allosteric inhibition; Environmentally sensitive fluorophores; Inter-domain interactions; Protein kinases; 540; 570
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APA (6th Edition):
Fang, Z. (2013). Development of a conformation sensitive assay for the detection of inter-domain interactions mediated by allosteric akt inhibitors. (Doctoral Dissertation). Technische Universität Dortmund. Retrieved from http://dx.doi.org/10.17877/DE290R-5596
Chicago Manual of Style (16th Edition):
Fang, Zhizhou. “Development of a conformation sensitive assay for the detection of inter-domain interactions mediated by allosteric akt inhibitors.” 2013. Doctoral Dissertation, Technische Universität Dortmund. Accessed January 15, 2021.
http://dx.doi.org/10.17877/DE290R-5596.
MLA Handbook (7th Edition):
Fang, Zhizhou. “Development of a conformation sensitive assay for the detection of inter-domain interactions mediated by allosteric akt inhibitors.” 2013. Web. 15 Jan 2021.
Vancouver:
Fang Z. Development of a conformation sensitive assay for the detection of inter-domain interactions mediated by allosteric akt inhibitors. [Internet] [Doctoral dissertation]. Technische Universität Dortmund; 2013. [cited 2021 Jan 15].
Available from: http://dx.doi.org/10.17877/DE290R-5596.
Council of Science Editors:
Fang Z. Development of a conformation sensitive assay for the detection of inter-domain interactions mediated by allosteric akt inhibitors. [Doctoral Dissertation]. Technische Universität Dortmund; 2013. Available from: http://dx.doi.org/10.17877/DE290R-5596
Western Michigan University
28.
Wajira Ariyadasa, Liyana A.
Size-Dependent Interactions of Metal Nanoparticles with Fluorophores and Semiconductors.
Degree: PhD, Chemistry, 2014, Western Michigan University
URL: https://scholarworks.wmich.edu/dissertations/235
► In recent years, nanoscale metallic particles have gained considerable interest due to their potential applications in advanced technology. Despite such interest, synthetic procedures that…
(more)
▼ In recent years, nanoscale metallic particles have gained considerable interest due to their potential applications in advanced technology. Despite such interest, synthetic procedures that produce gram-scale, well-defined metallic nanoparticles with controlled size and shape, especially with diameters less than 5 nm remains a challenge. Our work has focused on developing synthetic procedures that produce well-defined platinum and palladium metal nanoparticles in the 1-5 nm size range. Thioether ligands were used as stabilizers and resulted in metal nanoparticles with controlled size. The nanoparticles were characterized using transmission electron microscopy (TEM), x-ray diffraction (XRD), selected area electron diffraction (SAED), x-ray photoelectron spectroscopy (XPS), electrochemistry and high-resolution transmission electron microscopy (HRTEM). Unique size-dependent electronic properties were found to arise when the particle diameter was less than 5 nm. We studied the changes in electronic properties of
fluorophores and semiconductor nanoparticles brought in close proximity to the metal nanoparticles. The interaction of metal nanoparticles with the
fluorophores was found to be size-dependent involving a surface energy transfer mechanism. Furthermore, the metal nanoparticles were found to store multiple electrons that were effective in the catalytic reduction of environmental pollutants.
Advisors/Committee Members: Dr. Sherine O. Obare, Dr. Ekkehard Sinn, Dr. Andre Venter.
Subjects/Keywords: Metal nanoparticles; synthesis; size dependent; electron transfer; semiconductor; fluorophores; Materials Chemistry; Nanoscience and Nanotechnology
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APA ·
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APA (6th Edition):
Wajira Ariyadasa, L. A. (2014). Size-Dependent Interactions of Metal Nanoparticles with Fluorophores and Semiconductors. (Doctoral Dissertation). Western Michigan University. Retrieved from https://scholarworks.wmich.edu/dissertations/235
Chicago Manual of Style (16th Edition):
Wajira Ariyadasa, Liyana A. “Size-Dependent Interactions of Metal Nanoparticles with Fluorophores and Semiconductors.” 2014. Doctoral Dissertation, Western Michigan University. Accessed January 15, 2021.
https://scholarworks.wmich.edu/dissertations/235.
MLA Handbook (7th Edition):
Wajira Ariyadasa, Liyana A. “Size-Dependent Interactions of Metal Nanoparticles with Fluorophores and Semiconductors.” 2014. Web. 15 Jan 2021.
Vancouver:
Wajira Ariyadasa LA. Size-Dependent Interactions of Metal Nanoparticles with Fluorophores and Semiconductors. [Internet] [Doctoral dissertation]. Western Michigan University; 2014. [cited 2021 Jan 15].
Available from: https://scholarworks.wmich.edu/dissertations/235.
Council of Science Editors:
Wajira Ariyadasa LA. Size-Dependent Interactions of Metal Nanoparticles with Fluorophores and Semiconductors. [Doctoral Dissertation]. Western Michigan University; 2014. Available from: https://scholarworks.wmich.edu/dissertations/235
29.
Rémond, Maxime.
Développement de nouveaux chromophores dipolaires pour l'imagerie de fluorescence et l'imagerie photoacoustique : Design and synthesis of new dipolar chromophores for fluorescence imaging and photoacoustic imaging.
Degree: Docteur es, Chimie, 2018, Lyon
URL: http://www.theses.fr/2018LYSEN062
► Cette thèse traite de la conception, la synthèse et la caractérisation de chromophores dipolaires D-π-A pour l’imagerie de fluorescence et l’imagerie photoacoustique.La première partie est…
(more)
▼ Cette thèse traite de la conception, la synthèse et la caractérisation de chromophores dipolaires D-π-A pour l’imagerie de fluorescence et l’imagerie photoacoustique.La première partie est consacrée à la synthèse et à l’étude de nouveaux fluorophores émettant à l’état solide dans le proche infrarouge, pour l’imagerie de fluorescence biphotonique. Afin d’améliorer les propriétés optiques à l’état solide, nous avons exploré différentes pistes en modifiant d’abord le groupement D donneur d’électron, puis le groupement A accepteur et enfin le pont π conjugué de nos structures dipolaires.La seconde partie concerne l’imagerie photoacoustique. Cette imagerie, basée sur une excitation optique et une détection acoustique, nécessite une forte absorption dans le proche infrarouge. Une des stratégies pour effectuer ce décalage bathochrome de l’absorption a été incorporation de groupements thiophènes à faible aromaticité, afin d’augmenter la délocalisation du système π. Parallèlement, nous avons aussi étudiés des hémicyanines, présentant de fortes absorptions au-delà de 650 nm.Enfin, les colorants ont été formulés en nanoparticules organiques afin d’acquérir une solubilité aqueuse pour leur application en imagerie. Deux types de nanoparticules ont été étudiés : la nanoprécipitation des colorants en présence d’un agent tensioactif, ainsi que l’encapsulation des colorants dans une matrice polymérique amphiphile stabilisée par une couche de silice. Les meilleurs chromophores ont permis l’acquisition d’images de cellules par microscopie biphotonique ainsi que d’images photoacoustiques de circuits microfluidiques et de la microvascularisation de souris in vivo.
This thesis is focused on the conception, synthesis and characterization of dipolar dyes D-π-A for fluorescence imaging and photoacoustic imaging.The first part aim to synthesize and to study new fluorophores emitting in the solid-state in the near-infrared for fluorescence imaging with two-photon excitation. In order to improve the optical properties in the solid-state, we first explored various electron donating groups D, then acceptors A and finally we modified the π bridge. In a second part, we focused on dyes for photoacoustic imaging. This technique is based on an optical excitation and an acoustic detection. It requires a strong absorption in the biological window. To red shift the absorption we incorporated a thiophene bridge with low aromaticity to increase the delocalization. We also studied several hemicyanines with strong absorption above 650 nm.Finally, dyes were formulated as water-soluble nanoparticles for their final application for imaging. Two types of nanoparticles were studied: nanoprecipitated dyes stabilized by a surfactant to increase the colloidal stability or encapsulated dyes in a amphiphilic polymer matrix stabilized with a silica shell. Those nanoparticles enabled cells imaging with biphotonic florescence imaging as well as photoacoustic imaging of a microfluidic chip and of the microvascularisation of mice ears in vivo.
Advisors/Committee Members: Andraud, Chantal (thesis director).
Subjects/Keywords: Fluorescence; Imagerie; Photoacoustique; AIE; Fluorophores; Proche infrarouge; Fluorescence; Imaging; Photoacoustic; AIE; Fluorophore; Near-infrared
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APA (6th Edition):
Rémond, M. (2018). Développement de nouveaux chromophores dipolaires pour l'imagerie de fluorescence et l'imagerie photoacoustique : Design and synthesis of new dipolar chromophores for fluorescence imaging and photoacoustic imaging. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2018LYSEN062
Chicago Manual of Style (16th Edition):
Rémond, Maxime. “Développement de nouveaux chromophores dipolaires pour l'imagerie de fluorescence et l'imagerie photoacoustique : Design and synthesis of new dipolar chromophores for fluorescence imaging and photoacoustic imaging.” 2018. Doctoral Dissertation, Lyon. Accessed January 15, 2021.
http://www.theses.fr/2018LYSEN062.
MLA Handbook (7th Edition):
Rémond, Maxime. “Développement de nouveaux chromophores dipolaires pour l'imagerie de fluorescence et l'imagerie photoacoustique : Design and synthesis of new dipolar chromophores for fluorescence imaging and photoacoustic imaging.” 2018. Web. 15 Jan 2021.
Vancouver:
Rémond M. Développement de nouveaux chromophores dipolaires pour l'imagerie de fluorescence et l'imagerie photoacoustique : Design and synthesis of new dipolar chromophores for fluorescence imaging and photoacoustic imaging. [Internet] [Doctoral dissertation]. Lyon; 2018. [cited 2021 Jan 15].
Available from: http://www.theses.fr/2018LYSEN062.
Council of Science Editors:
Rémond M. Développement de nouveaux chromophores dipolaires pour l'imagerie de fluorescence et l'imagerie photoacoustique : Design and synthesis of new dipolar chromophores for fluorescence imaging and photoacoustic imaging. [Doctoral Dissertation]. Lyon; 2018. Available from: http://www.theses.fr/2018LYSEN062
University of Houston
30.
Lirag, Rio Carlo Manalo 1983-.
Studies in Supramolecular Chemistry: Self-Sorting Systems, Parallel Reactions, and Differential Sensing Using Cruciform/Half-Cruciform Fluorophores.
Degree: PhD, Chemistry, 2015, University of Houston
URL: http://hdl.handle.net/10657/5368
► This dissertation presents different studies in supramolecular chemistry—the first three chapters deal with self-sorting of dynamic covalent libraries and one-pot parallel reactions, while the final…
(more)
▼ This dissertation presents different studies in supramolecular chemistry—the first three chapters deal with self-sorting of dynamic covalent libraries and one-pot parallel reactions, while the final two chapters describe the use of cruciform
fluorophores in the construction of differential sensing arrays and the synthesis and characterization of “half-cruciform”
fluorophores.
Chapter One. This chapter introduces the broad field of supramolecular chemistry with an emphasis on dynamic covalent chemistry (DCC). Notable applications of DCC and simplification of dynamic covalent libraries via self-sorting induced by chemical and physical stimuli is reviewed.
Chapter Two. Kinetic self-sorting of dynamic imine libraries, constructed from aromatic aldehydes and substituted anilines, was achieved through selective precipitation. The selectivity of the process is caused by the solubility differences among possible imines in the ethanol-water solvent mixtures used in precipitation.
Chapter Three. Parallel reactions are introduced and self-sorting event is extended to include several competing reversible reactions. Four simultaneous dehydration reactions: imine formation, acetal formation, ester formation, and tertiary alcohol dehydration, were shown to proceed simultaneously in one pot using para-toluenesulfonic acid as the catalyst.
Chapter Four. This chapter describes a protocol for discriminating structurally-related analytes using a differential sensing array constructed from 1,4-distyryl-2,5-bis(arylethynyl)benzene cruciform, benzobisoxazole cruciform, and benzobisoxazole-boronic acid hybrid sensors. This method relies on the differences of the emission colors of the cruciform solutions before and after analyte addition. Ten carboxylic acids, nine organoboronic acids, and twelve organic amines, and ureas were differentiated from each other using this procedure.
Chapter Five. Nine “half-cruciform”
fluorophores containing benzimidazole core were synthesized in two steps: oxidative condensation of 3-bromo-o-phenylenediamine with three commercially-available aromatic aldehydes, which installs the horizontal axis, followed by Sonogashira coupling with aryl alkynes, which installs the vertical axis. Computational studies show that donor/acceptor substituted half-cruciform can localize their frontier molecular orbitals. These compounds were found to be viable fluorescent sensors, as they showed significant changes in their UV/Vis absorbance and fluorescence properties upon addition of bases, acids, and small anions.
Advisors/Committee Members: Miljanić, Ognjen Š. (advisor), Baldelli, Steven (committee member), Cai, Chengzhi (committee member), Daugulis, Olafs (committee member), Varadarajan, Navin (committee member).
Subjects/Keywords: Self-sorting; Parallel reactions; Differential sensing; Cruciform molecules; Half-cruciform; Fluorophores; Supramolecular chemistry
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APA ·
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APA (6th Edition):
Lirag, R. C. M. 1. (2015). Studies in Supramolecular Chemistry: Self-Sorting Systems, Parallel Reactions, and Differential Sensing Using Cruciform/Half-Cruciform Fluorophores. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/5368
Chicago Manual of Style (16th Edition):
Lirag, Rio Carlo Manalo 1983-. “Studies in Supramolecular Chemistry: Self-Sorting Systems, Parallel Reactions, and Differential Sensing Using Cruciform/Half-Cruciform Fluorophores.” 2015. Doctoral Dissertation, University of Houston. Accessed January 15, 2021.
http://hdl.handle.net/10657/5368.
MLA Handbook (7th Edition):
Lirag, Rio Carlo Manalo 1983-. “Studies in Supramolecular Chemistry: Self-Sorting Systems, Parallel Reactions, and Differential Sensing Using Cruciform/Half-Cruciform Fluorophores.” 2015. Web. 15 Jan 2021.
Vancouver:
Lirag RCM1. Studies in Supramolecular Chemistry: Self-Sorting Systems, Parallel Reactions, and Differential Sensing Using Cruciform/Half-Cruciform Fluorophores. [Internet] [Doctoral dissertation]. University of Houston; 2015. [cited 2021 Jan 15].
Available from: http://hdl.handle.net/10657/5368.
Council of Science Editors:
Lirag RCM1. Studies in Supramolecular Chemistry: Self-Sorting Systems, Parallel Reactions, and Differential Sensing Using Cruciform/Half-Cruciform Fluorophores. [Doctoral Dissertation]. University of Houston; 2015. Available from: http://hdl.handle.net/10657/5368
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Open Access Theses and Dissertations
