subject:(Fluorescence)

1. Huang, Da. Small Molecule Sensing by Local pH Modulation.

Degree: 2013, Texas Digital Library

URL: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658

► Our laboratory has previously invented and characterized a technique for studying ligand-receptor interactions on supported lipid bilayers (SLBs) without fluorescently labeled analytes. This technique utilizes… (more)

▼ Our laboratory has previously invented and characterized a technique for studying ligand-receptor interactions on supported lipid bilayers (SLBs) without fluorescently labeled analytes. This technique utilizes a pH-sensitive dye labeled phospholipid, ortho-rhodamine B conjugated POPE (1-hexadecanoyl-2-(9Z-octa-decenoyl)-sn-glycero-3-phosphoethanolamine), as a membrane imbedded reporter molecule to monitor ligand-receptor interactions on SLBs by measuring the fluorescence intensity change. Also, this technique has been demonstrated to operate as either a ???turn-on??? or a ???turn-off??? sensor depending on the analytes to be detected. Based on these preliminary studies, it is necessary to give further development and application for this new technique. Firstly, as a potential application of this sensing technique, we utilized this sensing platform to detect the interactions between tetracaine, a positively charged small molecule used as a local anesthetic drug, and planar supported lipid bilayers (SLBs). Studies with membranes composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) yielded an equilibrium dissociation constant value of Kd = 180??47 ??m for this small molecule-membrane interaction. And the influences of cholesterol, phosphatidylethanolamine, and three negatively charged lipids on the interaction between tetracaine and the bilayers have been studied. Secondly, in order to fully develop the power of this local pH modulation technique, it has been extended to a homogeneous platform from an SLB based heterogeneous platform. Specifically, a pH sensitive dye, 5-(and-6)-carboxyfluorescein, was conjugated to free lysine residues on the surfaces of designated capture proteins. The fluorescein intensity was found to change upon target molecule binding. The assay was used to follow the binding of immunoglobulin G (IgG) to streptavidin, thiamin monophosphate (ThMP) to thiamin binding protein A (TbpA), and Ca^(2+) to calmodulin (CaM). Finally, as an instrumental development for this sensing platform, an inexpensive fluorescence detector has been built as a cheap substitute for the epi-fluorescence microscope. This device is composed of a compact camera, a schott glass color filter, and a green (532 nm) laser pointer. This device has been tested to be able to obtain fluorescence images with decent signal to noise ratio. And this device has been demonstrated in measuring tetracaine-POPC SLB interaction using local pH modulation method. Advisors/Committee Members: Cremer, Paul S (advisor).

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Huang, D. (2013). Small Molecule Sensing by Local pH Modulation. (Thesis). Texas Digital Library. Retrieved from http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Da. “Small Molecule Sensing by Local pH Modulation.” 2013. Thesis, Texas Digital Library. Accessed February 27, 2020. http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Da. “Small Molecule Sensing by Local pH Modulation.” 2013. Web. 27 Feb 2020.

Vancouver:

Huang D. Small Molecule Sensing by Local pH Modulation. [Internet] [Thesis]. Texas Digital Library; 2013. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang D. Small Molecule Sensing by Local pH Modulation. [Thesis]. Texas Digital Library; 2013. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Delft University of Technology

2. Kurvink, J.W. An Implantable Chip for Time Resolved Fluorescence Detection:.

Degree: 2010, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:ca67021d-9277-4366-98cf-9d33c7e04479

► Photodynamic therapy (PDT) can be used to treat all kinds of (pre-)malignant and non malignant cells, and has some great advantages in comparison with conventional… (more)

▼ Photodynamic therapy (PDT) can be used to treat all kinds of (pre-)malignant and non malignant cells, and has some great advantages in comparison with conventional treatments, like radiotherapy and chemotherapy. To be able to perform PDT one needs three things to be present in a cell: photosensitizer, light of a specific wavelength, and oxygen. A photosensitizer is a specially designed drug that that is activated by light of a specific wavelength. When activated, the photosensitizer reacts with oxygen, which leads to cell death. The cell death can occur in two ways: necrosis and apoptosis. A new type of PDT has been introduced recently in the treatment of brain tumors, which is called metronomic PDT (mPDT). The principle of mPDT is similar to that of PDT, but the difference is the length of the treatment. mPDT works with a lower light dose and a longer treatment time compared to conventional PDT. This ensures that the cells dies in a natural way (apoptosis) and do not cause inflammations, which happens in normal PDT. To be able to perform mPDT on a human brain, it is necessary that the treatment can be monitored. For this purpose information on the state of the photosensitizer is necessary. An important aspect of the photosensitizer is the fluorescence, which occurs upon light absorption by the photosensitizer and is related to electronic transition from an excited state to the ground state of the used molecule. From the fluorescence reflection spectrum one can determine the concentration and interaction with cells, as well as localisation and configuration of the photosensitizer. The photosensitizers used for the treatment have a lifetime from 3.5ns to 10ns. To measure the lifetime of the photosensitizer we need to be able to reconstruct the exponential decay of the fluorescence reflection spectrum. In order to do this we use a LED to excite the photosensitizer and a photodiode for detection of the fluorescence reflection spectrum. We integrate the photocurrent on the parasitic capacitor of the photodiode, to make sure the system is fast. When the signal is integrated once, it holds no usable information other than the total charge integrated. For this purpose a tune-able delay circuit has been designed that can delay the beginning of the measurement, which allows us to change the integration window. If we now integrate the same signal several times, while changing the starting time for each integration, we can reconstruct the exponential decay of the fluorescence reflection spectrum. The designed chip houses the timing circuit for the tune-able delay, as well as a photodiode for light detection, and an amplifier to amplify the signal enough for readout with an AD-converter. There is also space for a LED on the chip, which is also controlled by the timing circuit to ensure correct on and off switching of the LED. The chip is designed in such a way that it can work with a external light source as well. The measurements are done without a photosensitizer because they are to expansive to use before proper testing of… Advisors/Committee Members: French, P.J., Margallo Balbás, E..

Subjects/Keywords: Time Resolved Fluorescence; Fluorescence

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APA (6^th Edition):

Kurvink, J. W. (2010). An Implantable Chip for Time Resolved Fluorescence Detection:. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:ca67021d-9277-4366-98cf-9d33c7e04479

Chicago Manual of Style (16^th Edition):

Kurvink, J W. “An Implantable Chip for Time Resolved Fluorescence Detection:.” 2010. Masters Thesis, Delft University of Technology. Accessed February 27, 2020. http://resolver.tudelft.nl/uuid:ca67021d-9277-4366-98cf-9d33c7e04479.

MLA Handbook (7^th Edition):

Kurvink, J W. “An Implantable Chip for Time Resolved Fluorescence Detection:.” 2010. Web. 27 Feb 2020.

Vancouver:

Kurvink JW. An Implantable Chip for Time Resolved Fluorescence Detection:. [Internet] [Masters thesis]. Delft University of Technology; 2010. [cited 2020 Feb 27]. Available from: http://resolver.tudelft.nl/uuid:ca67021d-9277-4366-98cf-9d33c7e04479.

Council of Science Editors:

Kurvink JW. An Implantable Chip for Time Resolved Fluorescence Detection:. [Masters Thesis]. Delft University of Technology; 2010. Available from: http://resolver.tudelft.nl/uuid:ca67021d-9277-4366-98cf-9d33c7e04479

University of Oklahoma

3. Singh, Anuradha. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/318880

In conclusion, investigations were carried out for the development of next generation optoelectronic devices and novel real world applicable biosensors. Advisors/Committee Members: Hlaterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence spectroscopy; Fluorescence microscopy; Biosensors

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APA (6^th Edition):

Singh, A. (2012). CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/318880

Chicago Manual of Style (16^th Edition):

Singh, Anuradha. “CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2020. http://hdl.handle.net/11244/318880.

MLA Handbook (7^th Edition):

Singh, Anuradha. “CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.” 2012. Web. 27 Feb 2020.

Vancouver:

Singh A. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/11244/318880.

Council of Science Editors:

Singh A. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/318880

University of Georgia

4. Palang, Edwin Yparraguirre. The role of ingredients and processing conditions on marinade penetration, retention and color defects in cooked marinated chicken breast meat.

Degree: PhD, Food Science, 2004, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/palang_edwin_y_200405_phd

► Marination by vacuum tumbling is commonly practiced in the meat industry; however, the optimal temperature of the process to maximize marinade absorption and retention is… (more)

▼ Marination by vacuum tumbling is commonly practiced in the meat industry; however, the optimal temperature of the process to maximize marinade absorption and retention is yet to be defined. Furthermore, the role of marinade functional ingredients and pH of the marinade in alleviating problems with pink color in cooked meat and water retention during cooking is yet to be elucidated. For denaturation model study, myoglobin pigments from chicken gizzards were extracted. Observed results from the model study were validated in whole muscle and comminuted chicken meat. In addition, temperature of vacuum tumbling operation was optimized with respect to marinade pick- up, cook yield and expressible moisture. Also, trivalent Eu+3 was used to trace the penetration of marinade in chicken breast meat. Results showed that increasing concentration of salt and pH in marinade increased the persistence of the pink color in cooked meat. Furthermore, degree of denaturation of myoglobin pigments was not a determining factor for cooked meat color under the condition of higher ORP values in meat products. On the other hand, temperature of marination was found to be a significant factor in marinade penetration and retention in a vacuum tumbling process. Higher marination temperature promoted deeper penetration of marinade in the meat as traced by trivalent Eu+3. Consequently, marinade pick-up was found at higher temperature. However, cook yield was found highest when marination process was initially at a higher temperature followed by lowering to near refrigeration temperature. Amount of salt soluble protein extracted at lower temperature was higher compared to higher temperature of marination that may be a factor in higher marinade retention on cooking In conclusion, pink color incidence in cooked meat is dependent to the ORP value of meat during and after cooking. Pink color can be induced in a product that has the cooked meat color by altering the ORP by addition of a reducing agent. In addition, Eu+3 tracing of marinade penetration in meat showed marinade to be only near the surface of the chicken breast meat even at the elevated temperature of vacuum tumbling. Advisors/Committee Members: Romeo Toledo.

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Palang, E. Y. (2004). The role of ingredients and processing conditions on marinade penetration, retention and color defects in cooked marinated chicken breast meat. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/palang_edwin_y_200405_phd

Chicago Manual of Style (16^th Edition):

Palang, Edwin Yparraguirre. “The role of ingredients and processing conditions on marinade penetration, retention and color defects in cooked marinated chicken breast meat.” 2004. Doctoral Dissertation, University of Georgia. Accessed February 27, 2020. http://purl.galileo.usg.edu/uga_etd/palang_edwin_y_200405_phd.

MLA Handbook (7^th Edition):

Palang, Edwin Yparraguirre. “The role of ingredients and processing conditions on marinade penetration, retention and color defects in cooked marinated chicken breast meat.” 2004. Web. 27 Feb 2020.

Vancouver:

Palang EY. The role of ingredients and processing conditions on marinade penetration, retention and color defects in cooked marinated chicken breast meat. [Internet] [Doctoral dissertation]. University of Georgia; 2004. [cited 2020 Feb 27]. Available from: http://purl.galileo.usg.edu/uga_etd/palang_edwin_y_200405_phd.

Council of Science Editors:

Palang EY. The role of ingredients and processing conditions on marinade penetration, retention and color defects in cooked marinated chicken breast meat. [Doctoral Dissertation]. University of Georgia; 2004. Available from: http://purl.galileo.usg.edu/uga_etd/palang_edwin_y_200405_phd

5. Thitipone Suwunwong. Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones .

Degree: คณะวิทยาศาสตร์ ภาควิชาเคมี, 2010, Prince of Songkla University

URL: http://kb.psu.ac.th/psukb/handle/2010/7694

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Suwunwong, T. (2010). Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones . (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2010/7694

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suwunwong, Thitipone. “Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones .” 2010. Thesis, Prince of Songkla University. Accessed February 27, 2020. http://kb.psu.ac.th/psukb/handle/2010/7694.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suwunwong, Thitipone. “Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones .” 2010. Web. 27 Feb 2020.

Vancouver:

Suwunwong T. Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones . [Internet] [Thesis]. Prince of Songkla University; 2010. [cited 2020 Feb 27]. Available from: http://kb.psu.ac.th/psukb/handle/2010/7694.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suwunwong T. Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones . [Thesis]. Prince of Songkla University; 2010. Available from: http://kb.psu.ac.th/psukb/handle/2010/7694

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

6. Mabaso, Matsilele Aubrey. Detection and tracking of sports in fluorescence microscopy images.

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/10829

►

M.Ing. (Electrical and Electronic Engineering)

Advances in bio-imaging have triggered the development of a highly sophisticated imaging tool known as fluorescence microscopy. Fluorescence microscopy is… (more)

▼

M.Ing. (Electrical and Electronic Engineering)

Advances in bio-imaging have triggered the development of a highly sophisticated imaging tool known as fluorescence microscopy. Fluorescence microscopy is used in many biological applications to visualize sub-cellular processes and gives the ability to image three-dimensional (3D) structures in living cells. The use of fluorescence microscopy and specific staining methods make biological molecules appear as bright spots in image data. The analysis of fluorescence microscopy images requires the detection and tracking of hundreds spots in image data and is of great importance for biologists to better understand cell functions. However, the analysis of these data is still performed manually in most biological laboratories worldwide. The manual analysis of these data is both time consuming, laborious and susceptible to human errors. Several computer-based algorithms have been proposed for the detection and tracking of spots in microscopy images. Most of these methods were validated on limited image data and relatively few studies have been performed for the comparison of these methods in real applications. This study quantitatively compared the performance of four detection and two tracking methods applied in microscopy images for the analysis of bright spots. The performance of the algorithms was validated on both synthetic and real images. The synthetic images offered a better way of validating algorithms against ground truth reference results. Results indicate that there are major differences in algorithm performance for both detection and tracking. In the detection results the Isotropic Undecimated Wavelet Transform (IUWT) and the Laplacian of Gaussian (LoG) achieved better results than the other methods in comparison when values are considered. The tracking results indicate that the Interacting Multiple Model (IMM) method achieved better results than the Feature Point Tracking (FPT) method when Jaccard Similarity Scores (JSC) are considered.

Subjects/Keywords: Fluorescence microscopy

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APA (6^th Edition):

Mabaso, M. A. (2014). Detection and tracking of sports in fluorescence microscopy images. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/10829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mabaso, Matsilele Aubrey. “Detection and tracking of sports in fluorescence microscopy images.” 2014. Thesis, University of Johannesburg. Accessed February 27, 2020. http://hdl.handle.net/10210/10829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mabaso, Matsilele Aubrey. “Detection and tracking of sports in fluorescence microscopy images.” 2014. Web. 27 Feb 2020.

Vancouver:

Mabaso MA. Detection and tracking of sports in fluorescence microscopy images. [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/10210/10829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mabaso MA. Detection and tracking of sports in fluorescence microscopy images. [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/10829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

7. Freese, Margaret Helene. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.

Degree: 2015, University of Johannesburg

URL: http://hdl.handle.net/10210/14701

►

M.Sc. (Chemistry)

The aim of this investigation was to study, and if possible, to correct curvature of calibration lines when solutions are analysed by X-ray… (more)

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Freese, M. H. (2015). Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/14701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freese, Margaret Helene. “Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.” 2015. Thesis, University of Johannesburg. Accessed February 27, 2020. http://hdl.handle.net/10210/14701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freese, Margaret Helene. “Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.” 2015. Web. 27 Feb 2020.

Vancouver:

Freese MH. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. [Internet] [Thesis]. University of Johannesburg; 2015. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/10210/14701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freese MH. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. [Thesis]. University of Johannesburg; 2015. Available from: http://hdl.handle.net/10210/14701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Valm, Alex Mihkel. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.

Degree: PhD, Pathobiology, 2012, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:297560/

► Just as the phenotypically different cells that make up multicellular organisms are distributed in tissues with structures that embody specific functions, microbial cells with different… (more)

▼ Just as the phenotypically different cells that make up multicellular organisms are distributed in tissues with structures that embody specific functions, microbial cells with different metabolic functions form unique spatial structures and coordinate their activities as multicellular units, e.g. biofilms. Fundamentally, microbial communities differ from eukaryotic tissues because their cellular constituents may be genetically distinct; in fact, up to hundreds of different species may be present in a single biofilm. Any number of probes may be designed to identify the different species present in a community; however, the ability to unambiguously distinguish more than a few different labels in a single fluorescence image has been severely hampered by the excitation cross talk and signal bleed-through of fluorophores with highly overlapping emission spectra. Reported in this thesis is a fluorescence labeling, imaging, and analysis method to greatly expand the number of identifiable labels in a single image, which we call Combinatorial Labeling and Spectral Imaging (CLASI). Application of our CLASI technique to human dental plaque using fluorescence in situ hybridization (FISH) enabled the first quantitative analysis of the spatial distribution of 15 different taxa of microbes in a biofilm. Proximity analysis was used to determine the frequency of inter- and intrataxon cell-to-cell associations, which revealed statistically significant intertaxon pairings. Cells of the genera Prevotella and Actinomyces showed the most interspecies associations, suggesting a central role for these genera in establishing and maintaining biofilm complexity. In a proof-of-principle experiment, we further demonstrate that we can distinguish 120 differently labeled E. coli in a mixture labeled with binary combinations of 16 fluorophores using a novel linear unmixing algorithm constrained to identify specific combinations of fluorophores. Our results provide an initial systems-level structural analysis of biofilm organization and we further believe that the CLASI approach will be useful for the architectural analysis of many complex molecular structures within cells. Advisors/Committee Members: Oldenbourg, Rudolf (Director), Borisy, Gary (Director), Atwood, Walter (Reader), Reichner, Jonathan (Reader), Danuser, Gaudenz (Reader).

Subjects/Keywords: fluorescence imaging

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APA (6^th Edition):

Valm, A. M. (2012). Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:297560/

Chicago Manual of Style (16^th Edition):

Valm, Alex Mihkel. “Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.” 2012. Doctoral Dissertation, Brown University. Accessed February 27, 2020. https://repository.library.brown.edu/studio/item/bdr:297560/.

MLA Handbook (7^th Edition):

Valm, Alex Mihkel. “Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.” 2012. Web. 27 Feb 2020.

Vancouver:

Valm AM. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. [Internet] [Doctoral dissertation]. Brown University; 2012. [cited 2020 Feb 27]. Available from: https://repository.library.brown.edu/studio/item/bdr:297560/.

Council of Science Editors:

Valm AM. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. [Doctoral Dissertation]. Brown University; 2012. Available from: https://repository.library.brown.edu/studio/item/bdr:297560/

9. Nekongo, Emmanuel Etum. Development of fluorescent pH indicator, fluorogenic protecting group, photoactivatable nucleophilic substitution (S[subscript N]1-type) reaction and photoactivatable fluorophores for biological applications.

Degree: PhD, Chemistry, 2012, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/nekongo_emmanuel_e_201208_phd

► In aqueous and alcohol solutions, colorless and non-fluorescent 9-aryl-9H-xanthen-9-ols equilibrate with brightly colored and fluorescent 9-arylxanthylium cations. The position of this equilibrium depends only on… (more)

▼ In aqueous and alcohol solutions, colorless and non-fluorescent 9-aryl-9H-xanthen-9-ols equilibrate with brightly colored and fluorescent 9-arylxanthylium cations. The position of this equilibrium depends only on hydronium ion concentration and is not affected by general acids or other ions. The pH of the equivalence point can be adjusted by substitution in the xanthenol core. 3,6-dialkoxy-9-(o-tolyl)-9-xanthenol undergoes dehydroxylation to form corresponding cation at biologically relevant pH = 6.5. 9-Aryl-9H-xanthen-9-ol – based pH indicators permit following acidity changes by both fluorescent and absorption spectroscopies. Non-fluorescent 3,6-bis(benzyloxy)-9-aryl-9-xanthenol undergoes photo-dehydroxylation with simultaneous photo-Claisen rearrangement upon irradiation in aqueous methanol to give fluorescent isomeric products. Alcohol substrates are simply caged by refluxing the parent 3,6-bis(benzyloxy)-9-aryl-9-xanthenol with the desired alcohol in acetonitrile and catalytic amounts of acetic acid or using the alcohol as neat solvent for less expensive substrates. Formation of fluorescent fluorescein derivatives can be used to monitor substrate release. Photolabile protecting groups can also be used to weaken or totally eradicate the fluorescence of a fluorophore. Irradiation of the caged dye removes the protection thereby revealing the fluorophore. We have effectively used 3-(hydroxymethyl)napthalen-2-ol as a photoremovable protecting group for fluorescein. The caged fluorophore is weakly fluorescent. Photolysis of the caged dye releases the fluorophore with a fluorescent quantum yield = 0.75 -0.93. Novel light-triggered SN1 strategy for the formation of glycosidic bond has been developed. The reaction is initiated by the photochemical cleavage of the bond between anomeric carbon and aglycone containing 9-aryl-9-fluorenol. Irradiation of 9-aryl-9-fluorenol derivatives bearing the tetrahydropyranyl moiety in 1,1,1,3,3,3-hexafluoro-2-propanol and tetrahydrofuran in a Royonet reactor at 300 nm presumably resulted in a short-lived 9-aryl-9-fluorenyl cation. The carbocation intramolecularly attacks the ether oxygen. This intramolecular attack of the ether by the initially formed carbocation leads to heterolysis of the C-O bond and the formation of oxonium ion. The oxonium ion thus generated is trapped preferentially with added nucleophile such as 2-phenyl ethanol. Advisors/Committee Members: Vladimir V. Popik.

Subjects/Keywords: fluorescence

…12 Figure 1.9: Fluorescence micrographs of NIH 3T3 fibroblast cells showing (A)… …spectroscopy, fluorescence spectroscopy is attractive because it is non-invasive, has greater… …the “off-state” of an indicator, fluorescence is quenched by electron transfer from the HOMO… …also confirmed by HRMS.24 Solutions containing only 1.9b show no measurable fluorescence… …xanthenol 1.9b show virtually no fluorescence. At lower pH values the emission band at 477 nm…

11 more images…

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APA (6^th Edition):

Nekongo, E. E. (2012). Development of fluorescent pH indicator, fluorogenic protecting group, photoactivatable nucleophilic substitution (S[subscript N]1-type) reaction and photoactivatable fluorophores for biological applications. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/nekongo_emmanuel_e_201208_phd

Chicago Manual of Style (16^th Edition):

Nekongo, Emmanuel Etum. “Development of fluorescent pH indicator, fluorogenic protecting group, photoactivatable nucleophilic substitution (S[subscript N]1-type) reaction and photoactivatable fluorophores for biological applications.” 2012. Doctoral Dissertation, University of Georgia. Accessed February 27, 2020. http://purl.galileo.usg.edu/uga_etd/nekongo_emmanuel_e_201208_phd.

MLA Handbook (7^th Edition):

Nekongo, Emmanuel Etum. “Development of fluorescent pH indicator, fluorogenic protecting group, photoactivatable nucleophilic substitution (S[subscript N]1-type) reaction and photoactivatable fluorophores for biological applications.” 2012. Web. 27 Feb 2020.

Vancouver:

Nekongo EE. Development of fluorescent pH indicator, fluorogenic protecting group, photoactivatable nucleophilic substitution (S[subscript N]1-type) reaction and photoactivatable fluorophores for biological applications. [Internet] [Doctoral dissertation]. University of Georgia; 2012. [cited 2020 Feb 27]. Available from: http://purl.galileo.usg.edu/uga_etd/nekongo_emmanuel_e_201208_phd.

Council of Science Editors:

Nekongo EE. Development of fluorescent pH indicator, fluorogenic protecting group, photoactivatable nucleophilic substitution (S[subscript N]1-type) reaction and photoactivatable fluorophores for biological applications. [Doctoral Dissertation]. University of Georgia; 2012. Available from: http://purl.galileo.usg.edu/uga_etd/nekongo_emmanuel_e_201208_phd

Montana State University

10. Tusell, JosÃƒÆ’Ã‚Â© RamÃƒÆ’Ã‚Â³n. Computation of tryptophan fluorescence quenching by amide and histidine.

Degree: College of Letters & Science, 2011, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/2458

► Tryptophan fluorescence quantum yield is widely used to follow protein folding for the villin headpiece subdomain (HP-35) and a synthetic peptide Ac-W-(A) ₃ -H +… (more)

▼ Tryptophan fluorescence quantum yield is widely used to follow protein folding for the villin headpiece subdomain (HP-35) and a synthetic peptide Ac-W-(A) ₃ -H + -NH ₂ (WH5). These biopolymers have a histidine residue, which is a potent quencher of tryptophan fluorescence, positioned four amino acids away from tryptophan. Experiments assumed that when folding occurs the fluorescence of tryptophan will be quenched by histidine due to the formation of an alpha helix. The reliability of folding and unfolding rate constants determined by tryptophan fluorescence has been called into question by several computational studies. A method to calculate the electron transfer matrix element was developed for different donor/acceptor systems. This method shows that the electron transfer matrix element is sensitive to orientation at close distances and that it does not follow a simple exponential decay with distance. This thesis improved the methods developed by Callis and coworker by conducting 100 ns long simulations for single tryptophan proteins and by modifying the calculation of the fluorescence quantum yield to account for heterogeneity in the calculated electron transfer rates. In addition the method was extended to calculate electron transfer rate constants for histidine quenching by conducting 1 microsecond long simulations of HP-35 and WH5. Calculated tryptophan fluorescence quantum yields for the single tryptophan proteins show better agreement with experiment than was previously reported. Simulations for HP-35 and WH5 indicate that the ability of histidine to quench the fluorescence of tryptophan is surprisingly controlled by the energy gap dependence on the distance that separates them. The energy gap dependence on this distance arises from water solvation around histidine. At large distances this solvation decreases the ability of histidine to accept an electron from tryptophan. Different tryptophan/histidine rotamers control this distance. Even when HP-35 is completely folded much of the time histidine does not quench tryptophan fluorescence contrary to the idea that histidine is only close when HP-35 is folded. The calculated fluorescence quantum yield is sensitive to the distribution of close and far conformations and the rate of exchange between these two conformations. This sensitivity gives credibility to the folding/unfolding rates derived from tryptophan fluorescence quantum yields. Advisors/Committee Members: Chairperson, Graduate Committee: Patrik R. Callis. (advisor).

Subjects/Keywords: Fluorescence.; Tryptophan.

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APA (6^th Edition):

Tusell, J. R. (2011). Computation of tryptophan fluorescence quenching by amide and histidine. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/2458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tusell, JosÃƒÆ’Ã‚Â© RamÃƒÆ’Ã‚Â³n. “Computation of tryptophan fluorescence quenching by amide and histidine.” 2011. Thesis, Montana State University. Accessed February 27, 2020. https://scholarworks.montana.edu/xmlui/handle/1/2458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tusell, JosÃƒÆ’Ã‚Â© RamÃƒÆ’Ã‚Â³n. “Computation of tryptophan fluorescence quenching by amide and histidine.” 2011. Web. 27 Feb 2020.

Vancouver:

Tusell JR. Computation of tryptophan fluorescence quenching by amide and histidine. [Internet] [Thesis]. Montana State University; 2011. [cited 2020 Feb 27]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2458.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tusell JR. Computation of tryptophan fluorescence quenching by amide and histidine. [Thesis]. Montana State University; 2011. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2458

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Macquarie University

11. Deng, Wei. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.

Degree: PhD, 2011, Macquarie University

URL: http://hdl.handle.net/1959.14/220460

►

"A thesis submitted to Macquarie University for the degree of Doctor of Philosophy, Department of Physics and Engineering, April 2011"

Bibliography: p. 153-183.

1. Acknowledgements… (more)

▼

"A thesis submitted to Macquarie University for the degree of Doctor of Philosophy, Department of Physics and Engineering, April 2011"

Bibliography: p. 153-183.

1. Acknowledgements – 2. List of publications – 3. Abstract – 4. Introduction – 5. Fluorescence and fluorescence instrumentation (review) – 6. Metal-enhanced fluorescence and its biological application (review) – 7. Enhanced flow cytometry-based bead immunoassays using metal nanostructures – 8. Metal nanostructure enhanced fluorescence of europium chelate BHHCT-Eu3+ applied to bioassays and time-gated bioimaging – 9. Upconversion phosphor nanoparticles and its application in bioassays (review) – 10. Upconversion in NaYF4 : Yb, Er nanoparticles amplified by metal nanostructures – 11. Ultrabright Eu-doped plasmonic [email protected] nanostructures: time-gated bioprobes with single particle sensitivity and negligible background – 12. Conclusion.

Over the last two decades, fluorescence based detection has become one of the leading sensing technologies in biomedical, biological and related sciences. Its sensitivity makes it possible to detect a single biomolecule through labeling with a suitable fluorophore. Two principal fluorophore properties, brightness and photostability, are fundamentally important to achieve a high level of sensitivity and in many conventional fluorophores these often fall short of the requirements. Among the methods used to improve the sensitivity of fluorescence detection, the metal-enhanced fluorescence (MEF) technique has been recently actively developed. The MEF phenomenon occurs when an excited fluorophore is located in close proximity to metals, and it is particularly pronounced near noble metal nanostructures. Electrons in such metal nanostructures exhibit strong resonances often located in the visible part of the spectrum (also known as surface plasmon resonance). They can interact with proximal fluorophores modifying their optical properties and producing increased quantum yield (fluorescence efficiency) and improved photostability. It has been experimentally demonstrated that the MEF technique can increase fluorescence intensity up to several hundreds times. The work through my PhD project mainly focus on siver nanostructures and its potential in fluorescence-based applications requiring very high sensitivity because their strong surface plasmon resonance in the visible matches the absorption and emission bands of most fluorophores. I began with synthesis for Ag nanostructure-coated silica beads in the solution-based platform as a new MEF substrate. My first study employed these nanostructures to enhance the fluorescence readout on individual silica beads. These Ag nanostructure were deposited on micrometer size silica beads. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay and AlexaFluor 430 labeling. Approximately 8.5-fold and 10.1-fold higher fluorescence intensities at 430 nm excitation were, respectively, observed from silvered 400 nm and 5 μm silica beads deposited on…

Advisors/Committee Members: Macquarie University. Dept. of Physics and Engineering.

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Deng, W. (2011). Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/220460

Chicago Manual of Style (16^th Edition):

Deng, Wei. “Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.” 2011. Doctoral Dissertation, Macquarie University. Accessed February 27, 2020. http://hdl.handle.net/1959.14/220460.

MLA Handbook (7^th Edition):

Deng, Wei. “Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.” 2011. Web. 27 Feb 2020.

Vancouver:

Deng W. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. [Internet] [Doctoral dissertation]. Macquarie University; 2011. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1959.14/220460.

Council of Science Editors:

Deng W. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. [Doctoral Dissertation]. Macquarie University; 2011. Available from: http://hdl.handle.net/1959.14/220460

Oregon State University

12. Hauswirth, William Walter. Fluorescence studies on the bases of the nucleic acids in solution at room temperature.

Degree: PhD, Chemistry, 1970, Oregon State University

URL: http://hdl.handle.net/1957/46021

► Fluorescence of adenine, guanine, thymine, cytosine, and uracil has been detected at room temperature in neutral aqueous solution using a digital signal accumulation technique and… (more)

▼ Fluorescence of adenine, guanine, thymine, cytosine, and uracil has been detected at room temperature in neutral aqueous solution using a digital signal accumulation technique and right angle fluorescence detection from solutions with absorbances between 0. 4 and 0. 7. The quantum yields are respectively 2.6 x 10⁻⁴, 3.0 x 10⁻⁴, 1 x 10⁻⁴ 0.8 x 10⁻⁴, and 0.5 x 10⁻⁴ when excited at their low energy absorption maxima. Singlet lifetimes calculated from these yields may mean a recalculation of excited state processes occurring in DNA which were previously based on low temperature data. Corrected relative emission spectra are presented and compared with reported low temperature data. The corrected relative excitation spectra all show significant differences from the absorption spectrum when both are determined under identical conditions of concentration and spectral bandwidth on the same instrument. This behavior is discussed in terms J. of a) n-π and π-π states, b) emission from a minor tautomer and c) kinetics of competing deactivation processes. From a development of c), rate constants for processes occurring from the 0' level are calculated for intersystem crossing, internal conversion, and (singlet) photochemical reaction in thymine and uracil. From solvent induced shifts of absorption and fluorescence spectra, the excited state dipole moment of thymine μe = 8. 0 ± 2. 20) and the π-π* nature of the transitions responsible for fluorescence are deduced. Consideration of the singlet lifetime of thymine in relation to the rates of excited state process implies that fluorescence may occur from a Franck-Condon excited state and this is discussed in terms of possible solute-solvent interactions. A study of the pH effects on the spectral shape, magnitude, and polarization of thymine emission concludes that the emitting states of J. ionized thymine are of a π-π* nature , that fluorescence may arise within the 1, 2,4, 5, and 6 π- conjugated system in the thymine molecule, and that excited state ionizations do not occur in the fluorescent forms of thymine. These deductions are discussed in relation to DNA excited state processes. Advisors/Committee Members: Daniels, Malcolm (advisor).

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Hauswirth, W. W. (1970). Fluorescence studies on the bases of the nucleic acids in solution at room temperature. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46021

Chicago Manual of Style (16^th Edition):

Hauswirth, William Walter. “Fluorescence studies on the bases of the nucleic acids in solution at room temperature.” 1970. Doctoral Dissertation, Oregon State University. Accessed February 27, 2020. http://hdl.handle.net/1957/46021.

MLA Handbook (7^th Edition):

Hauswirth, William Walter. “Fluorescence studies on the bases of the nucleic acids in solution at room temperature.” 1970. Web. 27 Feb 2020.

Vancouver:

Hauswirth WW. Fluorescence studies on the bases of the nucleic acids in solution at room temperature. [Internet] [Doctoral dissertation]. Oregon State University; 1970. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1957/46021.

Council of Science Editors:

Hauswirth WW. Fluorescence studies on the bases of the nucleic acids in solution at room temperature. [Doctoral Dissertation]. Oregon State University; 1970. Available from: http://hdl.handle.net/1957/46021

13. Suryadi, Jimmy. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE.

Degree: 2011, Wake Forest University

URL: http://hdl.handle.net/10339/33470

Our goal is to elucidate long range intramolecular signaling mechanisms in Escherichia coli methionyl tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.

Subjects/Keywords: Fluorescence

…Trichloroacetic acid) THF (Tetrahydrofuran) ix ABSTRACT FLUORESCENCE SPECTROSCOPY AND… …tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and… …fluorescence of MetRS changes upon binding two of its substrates: enhancement is observed when… …methionine binds and quenching occurs when tRNAMet binds. Because tryptophan fluorescence is… …that Trp253 in the active site is responsible for the increased fluorescence intensity. The…

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APA (6^th Edition):

Suryadi, J. (2011). FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/33470

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suryadi, Jimmy. “FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE.” 2011. Thesis, Wake Forest University. Accessed February 27, 2020. http://hdl.handle.net/10339/33470.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suryadi, Jimmy. “FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE.” 2011. Web. 27 Feb 2020.

Vancouver:

Suryadi J. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE. [Internet] [Thesis]. Wake Forest University; 2011. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/10339/33470.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suryadi J. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE. [Thesis]. Wake Forest University; 2011. Available from: http://hdl.handle.net/10339/33470

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kansas State University

14. Anderson, Richard. A comparison of intensities of indium and thallium sensitized fluorescene.

Degree: 1954, Kansas State University

URL: http://hdl.handle.net/2097/35868

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Anderson, R. (1954). A comparison of intensities of indium and thallium sensitized fluorescene. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/35868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Thesis, Kansas State University. Accessed February 27, 2020. http://hdl.handle.net/2097/35868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Web. 27 Feb 2020.

Vancouver:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Internet] [Thesis]. Kansas State University; 1954. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/2097/35868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Thesis]. Kansas State University; 1954. Available from: http://hdl.handle.net/2097/35868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oklahoma

15. Green, Nathaniel Scott. Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates.

Degree: PhD, 2013, University of Oklahoma

URL: http://hdl.handle.net/11244/319182

► Construction and aggregation of functionalized silica and gold nano-materials provides an opportunity to advance the field of fluorescence. The synthesis of gold nano-particles allows control… (more)

Subjects/Keywords: Fluorescence; Nanoparticles

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APA (6^th Edition):

Green, N. S. (2013). Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/319182

Chicago Manual of Style (16^th Edition):

Green, Nathaniel Scott. “Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates.” 2013. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2020. http://hdl.handle.net/11244/319182.

MLA Handbook (7^th Edition):

Green, Nathaniel Scott. “Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates.” 2013. Web. 27 Feb 2020.

Vancouver:

Green NS. Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates. [Internet] [Doctoral dissertation]. University of Oklahoma; 2013. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/11244/319182.

Council of Science Editors:

Green NS. Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates. [Doctoral Dissertation]. University of Oklahoma; 2013. Available from: http://hdl.handle.net/11244/319182

University of Oklahoma

16. Gunawardana, Gunawardana Buddika. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/319531

We believe this simple solution based approach we have developed to produce bright photostable metal fluorophore aggregates would have a greater potential to be used in the development of ultra-bright fluorescent tags for cellular imaging. Advisors/Committee Members: Halterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence; Nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gunawardana, G. B. (2012). Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/319531

Chicago Manual of Style (16^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2020. http://hdl.handle.net/11244/319531.

MLA Handbook (7^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Web. 27 Feb 2020.

Vancouver:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/11244/319531.

Council of Science Editors:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/319531

Kansas State University

17. Anderson, Richard. A comparison of intensities of indium and thallium sensitized fluorescene.

Degree: 1954, Kansas State University

URL: http://hdl.handle.net/2097/19298

Subjects/Keywords: Fluorescence

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Anderson, R. (1954). A comparison of intensities of indium and thallium sensitized fluorescene. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/19298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Thesis, Kansas State University. Accessed February 27, 2020. http://hdl.handle.net/2097/19298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Web. 27 Feb 2020.

Vancouver:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Internet] [Thesis]. Kansas State University; 1954. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/2097/19298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Thesis]. Kansas State University; 1954. Available from: http://hdl.handle.net/2097/19298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manitoba

18. Tavassoli, Marjan. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase).

Degree: Chemistry, 2013, University of Manitoba

URL: http://hdl.handle.net/1993/14918

► 1,6-Anhydro-N-acetylmuramic acid kinase (AnmK) is a homodimeric enzyme that can convert 1,6-Anhydro-N-acetylmuramic acid into N-acetylglucosamine-6-phosphate by consuming one molecule of ATP in murein recycling in… (more)

Subjects/Keywords: Fluorescence; AnmK

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APA (6^th Edition):

Tavassoli, M. (2013). Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase). (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/14918

Chicago Manual of Style (16^th Edition):

Tavassoli, Marjan. “Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase).” 2013. Masters Thesis, University of Manitoba. Accessed February 27, 2020. http://hdl.handle.net/1993/14918.

MLA Handbook (7^th Edition):

Tavassoli, Marjan. “Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase).” 2013. Web. 27 Feb 2020.

Vancouver:

Tavassoli M. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase). [Internet] [Masters thesis]. University of Manitoba; 2013. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1993/14918.

Council of Science Editors:

Tavassoli M. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase). [Masters Thesis]. University of Manitoba; 2013. Available from: http://hdl.handle.net/1993/14918

University of Oklahoma

19. Gunawardana, Gunawardana Buddika. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/318577

We believe this simple solution based approach we have developed to produce bright photostable metal fluorophore aggregates would have a greater potential to be used in the development of ultra-bright fluorescent tags for cellular imaging. Advisors/Committee Members: Halterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence; Nanoparticles

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gunawardana, G. B. (2012). Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/318577

Chicago Manual of Style (16^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2020. http://hdl.handle.net/11244/318577.

MLA Handbook (7^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Web. 27 Feb 2020.

Vancouver:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/11244/318577.

Council of Science Editors:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/318577

University of Waterloo

20. Wang, Jingqi. Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy.

Degree: 2019, University of Waterloo

URL: http://hdl.handle.net/10012/15148

► Foldamers represent a family of synthetic macromolecules which, like their biological counterparts, are able to adopt a well-defined conformation in solution. Oligoquinoline-carboxamides (Qn) are a… (more)

▼ Foldamers represent a family of synthetic macromolecules which, like their biological counterparts, are able to adopt a well-defined conformation in solution. Oligoquinoline-carboxamides (Qn) are a group of foldamers that adopt a helical conformation in solution. A series of Qn foldamers were prepared by chromatography-free large-scale synthesis and segment-doubling strategy. The C-terminal ester group of the Qn foldamers could be hydrolyzed to yield acid-functionalized foldamers (QnA) which could self-assemble into larger ((QnA)2-Na) complexes by metal coordination with a sodium cation. Moreover, the addition of a bis-acid functionalized tetramer (AQ2PQ2A) to a solution of (QnA)2-Na complexes resulted in insertion oligomeric products. To characterize these complexes in solution, both Qn and QnA were end-labeled with an oligo(phenylene vinylene) dye (OPV) at their N-terminus via a rigid amide bond to yield the OPV-Qn and OPV-QnA fluorescent equivalents. OPV was used to conduct time-resolved fluorescence anisotropy (TRFA) measurements on the OPV-Qn and OPV-QnA foldamers, the (OPV-QnA)2-Na complexes, and the OPV-Qn-Na-(AQ2PQ2A)n oligomers. Analysis of the TRFA of the OPV-Qn foldamers yielded the rotational time () of the fluorescent species, which was found to reflect the hydrodynamic volume (Vh) of the foldamers. The straight line obtained by plotting as a function of the number of (quinoline) units (NUs) demonstrated that the foldamers behaved in solution as rigid cylinders for all lengths examined. The linearity of the -vs-NU plot was employed as a calibration curve against which the rotational time of the QnA-complexes could be compared. Within experimental error, the rotational time of a Qn+m complex was found to equal the sum of the rotational times obtained for Qn and Qm. This result suggests that the complexation of two acid-functionalized oligoquinoline foldamers in solution generated a fully stacked foldamer with a NU equal to the sum of the NUs of its constituting elements. Hetero-complexes between OPV-Q8A and Q16A were also produced by adding a 10-fold excess of Q16A to an OPV-Q8A solution. Complexation was demonstrated by the  value of the mixture, that equaled that of an OPV-Q24 foldamer. Dilution experiments on a solution of OPV-Q8A-Na-Q16A complexes led to the dissociation of the complexes into their OPV-Q8A and Q16A constituting elements, as evidenced by the progressive decrease in  from the value obtained for OPV-Q24 to that of OPV-Q8 upon decreasing foldamer concentration. Similarly, the addition of increasing amounts of AQ2PQ2A to a solution of OPV-Q8A in chloroform resulted in an increase in  demonstrating the formation of complexes between OPV-Q8A and AQ2PQ2A until reached a plateau for large OPV-Q8A/AQ2PQ2A molar ratios. In the plateau region, the rotational time of the oligomeric complexes generated from OPV-Q8A and AQ2PQ2A stabilized by isobutyl or hexyl side chains was equal to that of an OPV-Q¬n foldamer with n equal to 24 or 30, respectively. The apparent absence of further…

Subjects/Keywords: Fluorescence Anisotropy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, J. (2019). Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/15148

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Jingqi. “Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy.” 2019. Thesis, University of Waterloo. Accessed February 27, 2020. http://hdl.handle.net/10012/15148.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Jingqi. “Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy.” 2019. Web. 27 Feb 2020.

Vancouver:

Wang J. Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy. [Internet] [Thesis]. University of Waterloo; 2019. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/10012/15148.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang J. Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy. [Thesis]. University of Waterloo; 2019. Available from: http://hdl.handle.net/10012/15148

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

21. Majchrowski, Thomas Francis. Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature.

Degree: MS, Dept. of Chemistry, 1971, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:11932

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Majchrowski, T. F. (1971). Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:11932

Chicago Manual of Style (16^th Edition):

Majchrowski, Thomas Francis. “Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature.” 1971. Masters Thesis, Michigan State University. Accessed February 27, 2020. http://etd.lib.msu.edu/islandora/object/etd:11932.

MLA Handbook (7^th Edition):

Majchrowski, Thomas Francis. “Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature.” 1971. Web. 27 Feb 2020.

Vancouver:

Majchrowski TF. Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature. [Internet] [Masters thesis]. Michigan State University; 1971. [cited 2020 Feb 27]. Available from: http://etd.lib.msu.edu/islandora/object/etd:11932.

Council of Science Editors:

Majchrowski TF. Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature. [Masters Thesis]. Michigan State University; 1971. Available from: http://etd.lib.msu.edu/islandora/object/etd:11932

University of Pennsylvania

22. Chiorazzo, Michael. Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer.

Degree: 2016, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1653

► Breast cancer is the most commonly diagnosed cancer in women and the number two cause of cancer associated death. Surgery is the first line of… (more)

▼ Breast cancer is the most commonly diagnosed cancer in women and the number two cause of cancer associated death. Surgery is the first line of defense in treating breast cancer and the success of surgery has been shown to predict the probability of cancer reoccurrence. Near Infrared (NIR) fluorescence guided surgery offers the potential to increase the accuracy of surgical resection by the use of fluorescence imaging agents targeted towards cancer. Cytosolic Phospholipase A2 (cPLA2) activity is enhanced in triple negative breast cancer and is associated with resistance to therapy in luminal-like cancers. The goal of this work is to develop activatable fluorophores targeted towards cPLA2 and evaluate their use for the molecular imaging of breast cancer in order to determine their potential as contrast agents for NIR fluorescence guided surgery. A series of tricyclic fluorophores were caged through esterification with fatty acids. Arachidonic acid was utilized to generate cPLA2 selective probes and palmitic acid was used to generate control probes. In vitro, probes were shown to activate in the presence of purified PLA2 and results were used to identify the most promising probe: DDAO arachidonate. Cellular fluorescence spectroscopy and microscopy showed DDAO arachidonate activated in breast cancer cell lines expressing cPLA2, where control probe did not. Modulation of cPLA2 activity through inhibition, induction, and genetic knockdown, resulted in corresponding changes in fluorescence generation. In a panel of breast cancer cell lines, cPLA2 expression, varied based on molecular subtype and correlated with DDAO arachidonate activation. Finally, DDAO arachidonate, when painted onto human triple negative tumor mouse xenografts, resulted in selective activation of tumor but not surrounding healthy tissue. Together, these results indicate DDAO arachidonate is a useful agent for measuring cPLA2 activity in both breast cancer cells and tumors and may offer the potential to aid in the fluorescence guided surgery of triple negative breast cancer.

Subjects/Keywords: Activatable Fluorescence; Breast Cancer; Fluorescence Guided Surgery

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chiorazzo, M. (2016). Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chiorazzo, Michael. “Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer.” 2016. Thesis, University of Pennsylvania. Accessed February 27, 2020. https://repository.upenn.edu/edissertations/1653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chiorazzo, Michael. “Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer.” 2016. Web. 27 Feb 2020.

Vancouver:

Chiorazzo M. Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer. [Internet] [Thesis]. University of Pennsylvania; 2016. [cited 2020 Feb 27]. Available from: https://repository.upenn.edu/edissertations/1653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chiorazzo M. Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer. [Thesis]. University of Pennsylvania; 2016. Available from: https://repository.upenn.edu/edissertations/1653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tulane University

23. Elfer, Katherine. The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology.

Degree: 2018, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:79052

►

Current microscopy-based tissue diagnostics, particularly hematoxylin and eosin (H&E) histology, requires multiple complex tissue processing steps: fixation, paraffin embedding, microtome sectioning, dying the tissue, and… (more)

▼

Current microscopy-based tissue diagnostics, particularly hematoxylin and eosin (H&E) histology, requires multiple complex tissue processing steps: fixation, paraffin embedding, microtome sectioning, dying the tissue, and imaging individual slides through a bright field microscope. The time and labor-intensive result of this process makes it unsuitable for patient point-of-care evaluation. Therefore, many bedside procedures are completed without efficient real-time analysis of tissue adequacy and diagnostic results are unnecessarily delayed. Additionally, research experiments that require information regarding changes to tissue morphology or function before proceeding to the next experimental phase are severely interrupted by histology processing in their workflow. Fluorescence histology, which relies on rapid fluorescent staining of tissue, optical sectioning microscopy, and image processing for digital viewing, can provide an inexpensive, non-destructive, 3-dimensional, and fast alternative to traditional histology and point-of-care screening protocols. The objective of this work is to further advance the concept of “fluorescence histology,” in which traditional histopathology preparation methods are replaced by optical-sectioning (in lieu of physical sectioning), sensitive and flexible fluorescence-based contrast (in lieu of chromophore-based contrast), and computational strategies to replicate traditional color-schemes. In this work, we demonstrate the development and use of a fluorescent analogue to H&E on fixed and frozen tissue sections and fresh human biopsies. This fluorescent analogue, DRAQ5 & eosin, is compared against the current single-agent, monochrome fluorescence histology system, and their effects on diagnostic downstream molecular analyses, including quantitative-PCR and immunohistochemistry, is evaluated. We create a methodology to develop and characterize fluorescent analogues for any histological stain, with demonstration using Masson’s Trichrome and Periodic Acid-Schiff, enabling the expansion of fluorescence histology for multiple applications. This work demonstrates the ability to improve point-of-care pathology and research by replacing destructive, incomplete, and time-consuming histology with fluorescence histology, which preserves the tissue for later analysis or experiments while providing accurate and rapid histology assessment.

1

Katherine Elfer

Advisors/Committee Members: Brown, Quincy (Thesis advisor), School of Science & Engineering Biomedical Engineering (Degree granting institution).

Subjects/Keywords: Fluorescence Histology; Fluorescence Microscopy; Image Processing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Elfer, K. (2018). The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:79052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elfer, Katherine. “The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology.” 2018. Thesis, Tulane University. Accessed February 27, 2020. https://digitallibrary.tulane.edu/islandora/object/tulane:79052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elfer, Katherine. “The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology.” 2018. Web. 27 Feb 2020.

Vancouver:

Elfer K. The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology. [Internet] [Thesis]. Tulane University; 2018. [cited 2020 Feb 27]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:79052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elfer K. The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology. [Thesis]. Tulane University; 2018. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:79052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Remy, Charlotte. Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules.

Degree: Docteur es, Chimie, 2016, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2016SACLN070

► La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les… (more)

▼ La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les résidus de pesticides tels que l’atrazine ainsi que la mélamine font notamment partie de ces molécules dangereuses pour la santé humaine. Ces deux molécules sont principalement dosées par des techniques lourdes et coûteuses comme la spectrométrie de masse, la chromatographie ou l’électrochimie. De même, la détection des amines biogéniques représente un intérêt sociétal. Elles sont produites par des bactéries durant la décarboxylation des acides aminés dans les cellules. Leur détection permet ainsi d’évaluer la contamination microbiologique et la dégradation potentielle d’un aliment. Elles sont aujourd’hui dosées par chromatographie en phase liquide ou en en phase gaz, par électrochromatographie capillaire et par spectroscopie UV-visible. Quelques exemples de détection par fluorescence ont déjà été décrits dans la littérature mais la nécessité de développer de nouveaux récepteurs fluorescents efficaces est bien réelle.La fluorescence est une technique qui offre de multiples avantages tels que la sensibilité, la sélectivité et un faible coût. De nombreuses sondes fluorescentes capables de détecter des métaux lourds ont été développées au laboratoire PPSM. Cependant, la détection de molécules neutres par fluorescence représente un défi supplémentaire en raison de la nature plus faible de l’interaction, comparée à celle entre espèces chargées.La première étape de cette thèse a été de concevoir et de synthétiser un ensemble de sondes moléculaires fluorescentes, aussi bien pour la détection de l’atrazine, de ses produits de dégradation et des dérivés de la mélamine que pour la détection des amines biogéniques. Des fluorophores dérivés de la molécule de maléimide, de naphthalimide et de l’acide barbiturique ont ainsi été développés pour sonder les dérivés de triazine en exploitant leur système de trois liaisons hydrogène pour la reconnaissance moléculaire. De même, un calix[6]arène fluorescent a été conçu pour déceler la présence des amines biogéniques qui provoqueront une réponse fluorescente par encapsulation dans le calixarène.La deuxième étape a consisté à étudier les propriétés photophysiques de ces sondes. La sonde Naphth-AlcyneOMe possède un rendement quantique élevé, s’est révélée fortement solvatochrome. Elle est de plus sensible à la déprotonation de sa fonction imide. Des études RMN et de modélisation moléculaire ont également été menées afin de caractériser les sondes de manière plus approfondie et de comprendre plus précisément leur réactivité. La spectroscopie RMN a confirmé l’interaction par liaison hydrogène entre les sondes maléimide et naphthalimides et la molécule d’atrazine. Elle a aussi mis en évidence l’encapsulation de l’heptylamine dans le calix[6]arène. Pour sa part, la modélisation moléculaire nous a permis de mieux comprendre la photophysique de la sonde Naphth-TriazoleOMe.Enfin, la capacité des sondes à détecter les divers… Advisors/Committee Members: Leray, Isabelle (thesis director).

Subjects/Keywords: Synthèse; Fluorescence; Sonde; Synthesis; Fluorescence; Probe

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Remy, C. (2016). Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLN070

Chicago Manual of Style (16^th Edition):

Remy, Charlotte. “Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed February 27, 2020. http://www.theses.fr/2016SACLN070.

MLA Handbook (7^th Edition):

Remy, Charlotte. “Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules.” 2016. Web. 27 Feb 2020.

Vancouver:

Remy C. Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2020 Feb 27]. Available from: http://www.theses.fr/2016SACLN070.

Council of Science Editors:

Remy C. Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLN070

Erasmus University Rotterdam

25. P.B.A.A. van Driel (Pieter). Fluorescence-guided Therapy in Oncology : Targeted Imaging and Photodynamic Therapy.

Degree: 2018, Erasmus University Rotterdam

URL: http://hdl.handle.net/1765/109908

► markdownabstractThis thesis demonstrates the use of fluorescence-guided therapy in oncology. First it shows the preclinical validation of two different targets and probes for fluorescence-guided surgery,… (more)

Subjects/Keywords: Fluorescence; fluorescence-guided surgery; photodynamic therapy

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APA (6^th Edition):

(Pieter), P. v. D. (2018). Fluorescence-guided Therapy in Oncology : Targeted Imaging and Photodynamic Therapy. (Thesis). Erasmus University Rotterdam. Retrieved from http://hdl.handle.net/1765/109908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

(Pieter), P.B.A.A. van Driel. “Fluorescence-guided Therapy in Oncology : Targeted Imaging and Photodynamic Therapy.” 2018. Thesis, Erasmus University Rotterdam. Accessed February 27, 2020. http://hdl.handle.net/1765/109908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

(Pieter), P.B.A.A. van Driel. “Fluorescence-guided Therapy in Oncology : Targeted Imaging and Photodynamic Therapy.” 2018. Web. 27 Feb 2020.

Vancouver:

(Pieter) PvD. Fluorescence-guided Therapy in Oncology : Targeted Imaging and Photodynamic Therapy. [Internet] [Thesis]. Erasmus University Rotterdam; 2018. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1765/109908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

(Pieter) PvD. Fluorescence-guided Therapy in Oncology : Targeted Imaging and Photodynamic Therapy. [Thesis]. Erasmus University Rotterdam; 2018. Available from: http://hdl.handle.net/1765/109908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

26. Assempour, Nazanin. Tuning the properties of protein-based biosensors by chromophore replacement strategies.

Degree: MS, Department of Chemistry, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/cxg94hp74q

► Optical imaging of membrane potentials has become an immensely popular approach to study neuronal activity. Over the past decades, a number of voltage indicators have… (more)

Subjects/Keywords: Fluorescence; Biosensor; Chromophore

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APA (6^th Edition):

Assempour, N. (2014). Tuning the properties of protein-based biosensors by chromophore replacement strategies. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cxg94hp74q

Chicago Manual of Style (16^th Edition):

Assempour, Nazanin. “Tuning the properties of protein-based biosensors by chromophore replacement strategies.” 2014. Masters Thesis, University of Alberta. Accessed February 27, 2020. https://era.library.ualberta.ca/files/cxg94hp74q.

MLA Handbook (7^th Edition):

Assempour, Nazanin. “Tuning the properties of protein-based biosensors by chromophore replacement strategies.” 2014. Web. 27 Feb 2020.

Vancouver:

Assempour N. Tuning the properties of protein-based biosensors by chromophore replacement strategies. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2020 Feb 27]. Available from: https://era.library.ualberta.ca/files/cxg94hp74q.

Council of Science Editors:

Assempour N. Tuning the properties of protein-based biosensors by chromophore replacement strategies. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cxg94hp74q

Texas A&M University

27. Longenberger, Polly Suzanne. Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2815

► A novel bioassay for the evaluation of plant water status was developed by Burke (2007). The research reported herein was designed to evaluate this new… (more)

▼ A novel bioassay for the evaluation of plant water status was developed by Burke (2007). The research reported herein was designed to evaluate this new protocol as a tool for use in cotton breeding programs for the identification of drought tolerant genotypes. Twenty genotypes were selected to represent diverse germplasm pools for a two-year field evaluation. Replicated tests were performed in Lubbock, TX and College Station, TX in 2005, 2006, and 2007. Dryland and irrigated treatments were administered in a split plot arrangement of a randomized complete block design. Fluorescence measurements were taken at mid-bloom and late bloom growth stages of growth. Source leaf tissue was harvested at predawn and subjected to high temperature incubation with fluorescence measurements subsequently taken hourly for five hours. Drought stressed plants had not mobilized their carbohydrate reserves from their source leaves overnight and thus maintained cell viability and therefore higher chlorophyll fluorescence values throughout the incubation with the opposite being true for nonstressed plants. Fiber lint yield and fiber properties were measured at the conclusion of the 2005 season in College Station and the 2006 season in College Station and Lubbock for comparison with the fluorescence data. Five genotypes, ?Acala 1517-99?, ?Deltapine 491? (PVP no. 200100159), ?Tamcot CAMD-E?, ?Tamcot 22? and TAM 89E-51, an unreleased breeding line, were selected based on field evaluation results in a preliminary study in 2005 to be included in a diallel analysis to determine the heritability of fluorescence measurements. Genotype x treatment effects complicated the classification of genotypic responses to drought. Few and inconsistent correlations were found among fluorescence values and lint yield or fiber properties. The diallel analysis did not identify general combining ability or specific combining ability effects for chlorophyll fluorescence measurements. Thus this procedure provides little potential in selecting plants for drought tolerance when plants are grown under field culture. Selection among Tamcot 22 and TAM 89E-51 plants for high and low genotypes according to fluorescence values did not yield progeny different from unselected Tamcot 22 and TAM 89E-51. Advisors/Committee Members: Smith, C. Wayne (advisor), Duke, Sara E. (committee member), McMichael, Bobbie L. (committee member), Rooney, William L. (committee member).

Subjects/Keywords: cotton; drought; fluorescence

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Longenberger, P. S. (2009). Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2815

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Longenberger, Polly Suzanne. “Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton.” 2009. Thesis, Texas A&M University. Accessed February 27, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-2815.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Longenberger, Polly Suzanne. “Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton.” 2009. Web. 27 Feb 2020.

Vancouver:

Longenberger PS. Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2815.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Longenberger PS. Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2815

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

28. Jongsma, Candice Gene. Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2894

► Most membrane proteins in eukaryotic cells are co-translationally integrated into the endoplasmic reticulum (ER) membrane at aqueous pores termed translocons. During multi-spanning membrane protein (MSMP)… (more)

▼ Most membrane proteins in eukaryotic cells are co-translationally integrated into the endoplasmic reticulum (ER) membrane at aqueous pores termed translocons. During multi-spanning membrane protein (MSMP) integration, the nascent polypeptide is threaded into the translocon pore where each successive transmembrane segment (TMS) is moved laterally through the translocon into the bilayer. The hydrophilic polypeptide segments on each side of the TMS are alternately directed into either the aqueous cytosol or the aqueous ER lumen. How is the ER membrane permeability barrier maintained during this process? For a single-spanning signal-cleaved membrane protein, nascent chain movement into the lumen occurs while an ion-tight ribosome-translocon junction prevents ion flow through the translocon pore. Prior to opening this junction to allow nascent chain movement into the cytosol, BiP (Hsp70 binding protein) effects closure at the lumenal end of the pore to maintain the membrane permeability barrier. To determine whether the ribosome and BiP alternately mediate pore closure during the integration of a MSMP, integration intermediates with nascent chains of different lengths were prepared with a fluorescent probe positioned in the nascent chain far inside the ribosomal tunnel. Nascent chain exposure to the cytosol or lumen was then detected by the collisional quenching of the probe by iodide ions located on either the cytosolic or lumenal side of the membrane. While the first TMS through the tunnel caused the ribosome-translocon junction to open, the second TMS elicited both the closure of this junction and the opening of the lumenal end of the pore. Movement of a third TMS through the tunnel caused the ribosome-translocon junction to re-open after closure of the lumenal end. Pore opening and closing occurred after each TMS was 4-7 residues from the peptidyltransferase center, irrespective of TMS location in the nascent chain. The ribosome treated all TMSs in the same manner, regardless of their individual sequence or their native orientation. The ER membrane permeability barrier is maintained by ribosome-translocon interactions during cotranslational MSMP integration. Advisors/Committee Members: JOHNSON, ARTHUR E. (advisor), RAUSHEL, FRANK M. (committee member), RUSSELL, DAVID H. (committee member), SCHOLTZ, J. MARTIN (committee member).

Subjects/Keywords: MEMBRANE; PROTEIN; FLUORESCENCE

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APA (6^th Edition):

Jongsma, C. G. (2009). Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jongsma, Candice Gene. “Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane.” 2009. Thesis, Texas A&M University. Accessed February 27, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-2894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jongsma, Candice Gene. “Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane.” 2009. Web. 27 Feb 2020.

Vancouver:

Jongsma CG. Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jongsma CG. Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

29. Wahlman, Judit. Fluorescent-detected retrotranslocation of an endoplasmic reticulum - associated degradation (ERAD) substrate in a mammalian in vitro system.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2019

► Secretory proteins that are unable to assemble into native proteins in the endoplasmic reticulum (ER) are transported back into the cytosol for degradation. Many cytosolic… (more)

▼ Secretory proteins that are unable to assemble into native proteins in the endoplasmic reticulum (ER) are transported back into the cytosol for degradation. Many cytosolic and ER resident proteins have been identified so far as being involved in this retrotranslocation process, but it is difficult to determine whether these proteins have a direct or indirect effect. Interpretations are further complicated if the loss of a specific protein is obscured by the presence of another protein that is partially or wholly redundant. To overcome these limitations, a mammalian in vitro system was developed that allowed to monitor retrotranslocation synchronously and in real time in the absence of concurrent translocation. To examine the roles of different components in ER-associated degradation (ERAD), well-defined and homogeneous mammalian ER microsomes were prepared biochemically by encapsulating a fluorescent-labeled ERAD substrate with specific lumenal components. After mixing ATP, specific cytosolic proteins, and specific fluorescence quenching agents with microsomes, substrate retrotranslocation was initiated. The rate of substrate efflux from microsomes was monitored spectroscopically and continuously in real time by the reduction in fluorescence intensity as the fluorescent substrates passed through the ER membrane and were exposed to the quenching agents. Retrotranslocation kinetics were not significantly altered by replacing all lumenal proteins with only protein disulfide isomerase, or all cytosolic proteins with only the 19S proteasome cap. Retrotranslocation was blocked by affinity-purified antibodies against Derlin1, but not by affinity-purified antibodies against Sec61? or by membrane-bound ribosomes. Since the substrate also photocrosslinked Derlin1, but not Sec61? or TRAM, retrotranslocation of this ERAD substrate apparently involves Derlin1, but not the translocon. By labeling either the C- or N-terminus, it was revealed that the N-terminus of one ERAD substrate leaves the ER lumen first. This finding suggests that the protein is retrotranslocated as a linear polymer in a preferred direction. When RRMs were reconstituted with a fluorescent-labeled ERAD substrate and various ions. Ca2+ ions in the ER lumen increased the rate and extent of retrotranslocation, while Ca2+ ions in the cytosol decreased retrotranslocation. This approach therefore provides the first direct evidence of the involvement and importance of specific ionic requirements for ERAD. Advisors/Committee Members: Johnson, Arthur E. (advisor), Reinhart, Gregory D. (committee member), Pace, C. Nick (committee member), Polymenis, Michael (committee member).

Subjects/Keywords: Fluorescence; endoplasmic reticulum

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wahlman, J. (2009). Fluorescent-detected retrotranslocation of an endoplasmic reticulum - associated degradation (ERAD) substrate in a mammalian in vitro system. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2019

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wahlman, Judit. “Fluorescent-detected retrotranslocation of an endoplasmic reticulum - associated degradation (ERAD) substrate in a mammalian in vitro system.” 2009. Thesis, Texas A&M University. Accessed February 27, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-2019.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wahlman, Judit. “Fluorescent-detected retrotranslocation of an endoplasmic reticulum - associated degradation (ERAD) substrate in a mammalian in vitro system.” 2009. Web. 27 Feb 2020.

Vancouver:

Wahlman J. Fluorescent-detected retrotranslocation of an endoplasmic reticulum - associated degradation (ERAD) substrate in a mammalian in vitro system. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 27]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2019.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wahlman J. Fluorescent-detected retrotranslocation of an endoplasmic reticulum - associated degradation (ERAD) substrate in a mammalian in vitro system. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2019

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Anna University

30. Rajasekaran R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.

Degree: Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy, 2015, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/33585

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Cancer is a dreaded disease and one of the leading causes of death newlineWorldwide The complete treatment of cancer is possible only when it is… (more)

Subjects/Keywords: Fluorescence spectroscopy; Worldwide

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

R, R. (2015). Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/33585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

R, Rajasekaran. “Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.” 2015. Thesis, Anna University. Accessed February 27, 2020. http://shodhganga.inflibnet.ac.in/handle/10603/33585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

R, Rajasekaran. “Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.” 2015. Web. 27 Feb 2020.

Vancouver:

R R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. [Internet] [Thesis]. Anna University; 2015. [cited 2020 Feb 27]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

R R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. [Thesis]. Anna University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations