subject:(Fluorescence)

1. Huang, Da. Small Molecule Sensing by Local pH Modulation.

Degree: 2013, Texas Digital Library

URL: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658

► Our laboratory has previously invented and characterized a technique for studying ligand-receptor interactions on supported lipid bilayers (SLBs) without fluorescently labeled analytes. This technique utilizes… (more)

▼ Our laboratory has previously invented and characterized a technique for studying ligand-receptor interactions on supported lipid bilayers (SLBs) without fluorescently labeled analytes. This technique utilizes a pH-sensitive dye labeled phospholipid, ortho-rhodamine B conjugated POPE (1-hexadecanoyl-2-(9Z-octa-decenoyl)-sn-glycero-3-phosphoethanolamine), as a membrane imbedded reporter molecule to monitor ligand-receptor interactions on SLBs by measuring the fluorescence intensity change. Also, this technique has been demonstrated to operate as either a ???turn-on??? or a ???turn-off??? sensor depending on the analytes to be detected. Based on these preliminary studies, it is necessary to give further development and application for this new technique. Firstly, as a potential application of this sensing technique, we utilized this sensing platform to detect the interactions between tetracaine, a positively charged small molecule used as a local anesthetic drug, and planar supported lipid bilayers (SLBs). Studies with membranes composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) yielded an equilibrium dissociation constant value of Kd = 180??47 ??m for this small molecule-membrane interaction. And the influences of cholesterol, phosphatidylethanolamine, and three negatively charged lipids on the interaction between tetracaine and the bilayers have been studied. Secondly, in order to fully develop the power of this local pH modulation technique, it has been extended to a homogeneous platform from an SLB based heterogeneous platform. Specifically, a pH sensitive dye, 5-(and-6)-carboxyfluorescein, was conjugated to free lysine residues on the surfaces of designated capture proteins. The fluorescein intensity was found to change upon target molecule binding. The assay was used to follow the binding of immunoglobulin G (IgG) to streptavidin, thiamin monophosphate (ThMP) to thiamin binding protein A (TbpA), and Ca^(2+) to calmodulin (CaM). Finally, as an instrumental development for this sensing platform, an inexpensive fluorescence detector has been built as a cheap substitute for the epi-fluorescence microscope. This device is composed of a compact camera, a schott glass color filter, and a green (532 nm) laser pointer. This device has been tested to be able to obtain fluorescence images with decent signal to noise ratio. And this device has been demonstrated in measuring tetracaine-POPC SLB interaction using local pH modulation method. Advisors/Committee Members: Cremer, Paul S (advisor).

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Huang, D. (2013). Small Molecule Sensing by Local pH Modulation. (Thesis). Texas Digital Library. Retrieved from http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Da. “Small Molecule Sensing by Local pH Modulation.” 2013. Thesis, Texas Digital Library. Accessed February 27, 2021. http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Da. “Small Molecule Sensing by Local pH Modulation.” 2013. Web. 27 Feb 2021.

Vancouver:

Huang D. Small Molecule Sensing by Local pH Modulation. [Internet] [Thesis]. Texas Digital Library; 2013. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang D. Small Molecule Sensing by Local pH Modulation. [Thesis]. Texas Digital Library; 2013. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oklahoma

2. Singh, Anuradha. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/318880

In conclusion, investigations were carried out for the development of next generation optoelectronic devices and novel real world applicable biosensors. Advisors/Committee Members: Hlaterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence spectroscopy; Fluorescence microscopy; Biosensors

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APA (6^th Edition):

Singh, A. (2012). CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/318880

Chicago Manual of Style (16^th Edition):

Singh, Anuradha. “CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2021. http://hdl.handle.net/11244/318880.

MLA Handbook (7^th Edition):

Singh, Anuradha. “CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.” 2012. Web. 27 Feb 2021.

Vancouver:

Singh A. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/11244/318880.

Council of Science Editors:

Singh A. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/318880

University of Waterloo

3. Wang, Jingqi. Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy.

Degree: 2019, University of Waterloo

URL: http://hdl.handle.net/10012/15148

► Foldamers represent a family of synthetic macromolecules which, like their biological counterparts, are able to adopt a well-defined conformation in solution. Oligoquinoline-carboxamides (Qn) are a… (more)

▼ Foldamers represent a family of synthetic macromolecules which, like their biological counterparts, are able to adopt a well-defined conformation in solution. Oligoquinoline-carboxamides (Qn) are a group of foldamers that adopt a helical conformation in solution. A series of Qn foldamers were prepared by chromatography-free large-scale synthesis and segment-doubling strategy. The C-terminal ester group of the Qn foldamers could be hydrolyzed to yield acid-functionalized foldamers (QnA) which could self-assemble into larger ((QnA)2-Na) complexes by metal coordination with a sodium cation. Moreover, the addition of a bis-acid functionalized tetramer (AQ2PQ2A) to a solution of (QnA)2-Na complexes resulted in insertion oligomeric products. To characterize these complexes in solution, both Qn and QnA were end-labeled with an oligo(phenylene vinylene) dye (OPV) at their N-terminus via a rigid amide bond to yield the OPV-Qn and OPV-QnA fluorescent equivalents. OPV was used to conduct time-resolved fluorescence anisotropy (TRFA) measurements on the OPV-Qn and OPV-QnA foldamers, the (OPV-QnA)2-Na complexes, and the OPV-Qn-Na-(AQ2PQ2A)n oligomers. Analysis of the TRFA of the OPV-Qn foldamers yielded the rotational time () of the fluorescent species, which was found to reflect the hydrodynamic volume (Vh) of the foldamers. The straight line obtained by plotting as a function of the number of (quinoline) units (NUs) demonstrated that the foldamers behaved in solution as rigid cylinders for all lengths examined. The linearity of the -vs-NU plot was employed as a calibration curve against which the rotational time of the QnA-complexes could be compared. Within experimental error, the rotational time of a Qn+m complex was found to equal the sum of the rotational times obtained for Qn and Qm. This result suggests that the complexation of two acid-functionalized oligoquinoline foldamers in solution generated a fully stacked foldamer with a NU equal to the sum of the NUs of its constituting elements. Hetero-complexes between OPV-Q8A and Q16A were also produced by adding a 10-fold excess of Q16A to an OPV-Q8A solution. Complexation was demonstrated by the  value of the mixture, that equaled that of an OPV-Q24 foldamer. Dilution experiments on a solution of OPV-Q8A-Na-Q16A complexes led to the dissociation of the complexes into their OPV-Q8A and Q16A constituting elements, as evidenced by the progressive decrease in  from the value obtained for OPV-Q24 to that of OPV-Q8 upon decreasing foldamer concentration. Similarly, the addition of increasing amounts of AQ2PQ2A to a solution of OPV-Q8A in chloroform resulted in an increase in  demonstrating the formation of complexes between OPV-Q8A and AQ2PQ2A until reached a plateau for large OPV-Q8A/AQ2PQ2A molar ratios. In the plateau region, the rotational time of the oligomeric complexes generated from OPV-Q8A and AQ2PQ2A stabilized by isobutyl or hexyl side chains was equal to that of an OPV-Q¬n foldamer with n equal to 24 or 30, respectively. The apparent absence of further…

Subjects/Keywords: Fluorescence Anisotropy

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APA (6^th Edition):

Wang, J. (2019). Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/15148

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Jingqi. “Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy.” 2019. Thesis, University of Waterloo. Accessed February 27, 2021. http://hdl.handle.net/10012/15148.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Jingqi. “Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy.” 2019. Web. 27 Feb 2021.

Vancouver:

Wang J. Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy. [Internet] [Thesis]. University of Waterloo; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10012/15148.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang J. Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy. [Thesis]. University of Waterloo; 2019. Available from: http://hdl.handle.net/10012/15148

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Thitipone Suwunwong. Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones .

Degree: คณะวิทยาศาสตร์ ภาควิชาเคมี, 2010, Prince of Songkla University

URL: http://kb.psu.ac.th/psukb/handle/2010/7694

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Suwunwong, T. (2010). Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones . (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2010/7694

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suwunwong, Thitipone. “Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones .” 2010. Thesis, Prince of Songkla University. Accessed February 27, 2021. http://kb.psu.ac.th/psukb/handle/2010/7694.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suwunwong, Thitipone. “Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones .” 2010. Web. 27 Feb 2021.

Vancouver:

Suwunwong T. Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones . [Internet] [Thesis]. Prince of Songkla University; 2010. [cited 2021 Feb 27]. Available from: http://kb.psu.ac.th/psukb/handle/2010/7694.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suwunwong T. Syntheses aand fluorescent properties of chalcone derivatives and heteroaryl chalcones . [Thesis]. Prince of Songkla University; 2010. Available from: http://kb.psu.ac.th/psukb/handle/2010/7694

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

5. Mabaso, Matsilele Aubrey. Detection and tracking of sports in fluorescence microscopy images.

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/10829

►

M.Ing. (Electrical and Electronic Engineering)

Advances in bio-imaging have triggered the development of a highly sophisticated imaging tool known as fluorescence microscopy. Fluorescence microscopy is… (more)

▼

M.Ing. (Electrical and Electronic Engineering)

Advances in bio-imaging have triggered the development of a highly sophisticated imaging tool known as fluorescence microscopy. Fluorescence microscopy is used in many biological applications to visualize sub-cellular processes and gives the ability to image three-dimensional (3D) structures in living cells. The use of fluorescence microscopy and specific staining methods make biological molecules appear as bright spots in image data. The analysis of fluorescence microscopy images requires the detection and tracking of hundreds spots in image data and is of great importance for biologists to better understand cell functions. However, the analysis of these data is still performed manually in most biological laboratories worldwide. The manual analysis of these data is both time consuming, laborious and susceptible to human errors. Several computer-based algorithms have been proposed for the detection and tracking of spots in microscopy images. Most of these methods were validated on limited image data and relatively few studies have been performed for the comparison of these methods in real applications. This study quantitatively compared the performance of four detection and two tracking methods applied in microscopy images for the analysis of bright spots. The performance of the algorithms was validated on both synthetic and real images. The synthetic images offered a better way of validating algorithms against ground truth reference results. Results indicate that there are major differences in algorithm performance for both detection and tracking. In the detection results the Isotropic Undecimated Wavelet Transform (IUWT) and the Laplacian of Gaussian (LoG) achieved better results than the other methods in comparison when values are considered. The tracking results indicate that the Interacting Multiple Model (IMM) method achieved better results than the Feature Point Tracking (FPT) method when Jaccard Similarity Scores (JSC) are considered.

Subjects/Keywords: Fluorescence microscopy

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APA (6^th Edition):

Mabaso, M. A. (2014). Detection and tracking of sports in fluorescence microscopy images. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/10829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mabaso, Matsilele Aubrey. “Detection and tracking of sports in fluorescence microscopy images.” 2014. Thesis, University of Johannesburg. Accessed February 27, 2021. http://hdl.handle.net/10210/10829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mabaso, Matsilele Aubrey. “Detection and tracking of sports in fluorescence microscopy images.” 2014. Web. 27 Feb 2021.

Vancouver:

Mabaso MA. Detection and tracking of sports in fluorescence microscopy images. [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10210/10829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mabaso MA. Detection and tracking of sports in fluorescence microscopy images. [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/10829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

6. Freese, Margaret Helene. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.

Degree: 2015, University of Johannesburg

URL: http://hdl.handle.net/10210/14701

►

M.Sc. (Chemistry)

The aim of this investigation was to study, and if possible, to correct curvature of calibration lines when solutions are analysed by X-ray… (more)

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Freese, M. H. (2015). Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/14701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freese, Margaret Helene. “Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.” 2015. Thesis, University of Johannesburg. Accessed February 27, 2021. http://hdl.handle.net/10210/14701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freese, Margaret Helene. “Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.” 2015. Web. 27 Feb 2021.

Vancouver:

Freese MH. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. [Internet] [Thesis]. University of Johannesburg; 2015. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10210/14701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freese MH. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. [Thesis]. University of Johannesburg; 2015. Available from: http://hdl.handle.net/10210/14701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Valm, Alex Mihkel. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.

Degree: PhD, Pathobiology, 2012, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:297560/

► Just as the phenotypically different cells that make up multicellular organisms are distributed in tissues with structures that embody specific functions, microbial cells with different… (more)

▼ Just as the phenotypically different cells that make up multicellular organisms are distributed in tissues with structures that embody specific functions, microbial cells with different metabolic functions form unique spatial structures and coordinate their activities as multicellular units, e.g. biofilms. Fundamentally, microbial communities differ from eukaryotic tissues because their cellular constituents may be genetically distinct; in fact, up to hundreds of different species may be present in a single biofilm. Any number of probes may be designed to identify the different species present in a community; however, the ability to unambiguously distinguish more than a few different labels in a single fluorescence image has been severely hampered by the excitation cross talk and signal bleed-through of fluorophores with highly overlapping emission spectra. Reported in this thesis is a fluorescence labeling, imaging, and analysis method to greatly expand the number of identifiable labels in a single image, which we call Combinatorial Labeling and Spectral Imaging (CLASI). Application of our CLASI technique to human dental plaque using fluorescence in situ hybridization (FISH) enabled the first quantitative analysis of the spatial distribution of 15 different taxa of microbes in a biofilm. Proximity analysis was used to determine the frequency of inter- and intrataxon cell-to-cell associations, which revealed statistically significant intertaxon pairings. Cells of the genera Prevotella and Actinomyces showed the most interspecies associations, suggesting a central role for these genera in establishing and maintaining biofilm complexity. In a proof-of-principle experiment, we further demonstrate that we can distinguish 120 differently labeled E. coli in a mixture labeled with binary combinations of 16 fluorophores using a novel linear unmixing algorithm constrained to identify specific combinations of fluorophores. Our results provide an initial systems-level structural analysis of biofilm organization and we further believe that the CLASI approach will be useful for the architectural analysis of many complex molecular structures within cells. Advisors/Committee Members: Oldenbourg, Rudolf (Director), Borisy, Gary (Director), Atwood, Walter (Reader), Reichner, Jonathan (Reader), Danuser, Gaudenz (Reader).

Subjects/Keywords: fluorescence imaging

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APA (6^th Edition):

Valm, A. M. (2012). Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:297560/

Chicago Manual of Style (16^th Edition):

Valm, Alex Mihkel. “Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.” 2012. Doctoral Dissertation, Brown University. Accessed February 27, 2021. https://repository.library.brown.edu/studio/item/bdr:297560/.

MLA Handbook (7^th Edition):

Valm, Alex Mihkel. “Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.” 2012. Web. 27 Feb 2021.

Vancouver:

Valm AM. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. [Internet] [Doctoral dissertation]. Brown University; 2012. [cited 2021 Feb 27]. Available from: https://repository.library.brown.edu/studio/item/bdr:297560/.

Council of Science Editors:

Valm AM. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. [Doctoral Dissertation]. Brown University; 2012. Available from: https://repository.library.brown.edu/studio/item/bdr:297560/

8. Suryadi, Jimmy. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE.

Degree: 2011, Wake Forest University

URL: http://hdl.handle.net/10339/33470

Our goal is to elucidate long range intramolecular signaling mechanisms in Escherichia coli methionyl tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.

Subjects/Keywords: Fluorescence

…Trichloroacetic acid) THF (Tetrahydrofuran) ix ABSTRACT FLUORESCENCE SPECTROSCOPY AND… …tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and… …fluorescence of MetRS changes upon binding two of its substrates: enhancement is observed when… …methionine binds and quenching occurs when tRNAMet binds. Because tryptophan fluorescence is… …that Trp253 in the active site is responsible for the increased fluorescence intensity. The…

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APA (6^th Edition):

Suryadi, J. (2011). FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/33470

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suryadi, Jimmy. “FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE.” 2011. Thesis, Wake Forest University. Accessed February 27, 2021. http://hdl.handle.net/10339/33470.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suryadi, Jimmy. “FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE.” 2011. Web. 27 Feb 2021.

Vancouver:

Suryadi J. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE. [Internet] [Thesis]. Wake Forest University; 2011. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10339/33470.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suryadi J. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE. [Thesis]. Wake Forest University; 2011. Available from: http://hdl.handle.net/10339/33470

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

9. Hauswirth, William Walter. Fluorescence studies on the bases of the nucleic acids in solution at room temperature.

Degree: PhD, Chemistry, 1970, Oregon State University

URL: http://hdl.handle.net/1957/46021

► Fluorescence of adenine, guanine, thymine, cytosine, and uracil has been detected at room temperature in neutral aqueous solution using a digital signal accumulation technique and… (more)

▼ Fluorescence of adenine, guanine, thymine, cytosine, and uracil has been detected at room temperature in neutral aqueous solution using a digital signal accumulation technique and right angle fluorescence detection from solutions with absorbances between 0. 4 and 0. 7. The quantum yields are respectively 2.6 x 10⁻⁴, 3.0 x 10⁻⁴, 1 x 10⁻⁴ 0.8 x 10⁻⁴, and 0.5 x 10⁻⁴ when excited at their low energy absorption maxima. Singlet lifetimes calculated from these yields may mean a recalculation of excited state processes occurring in DNA which were previously based on low temperature data. Corrected relative emission spectra are presented and compared with reported low temperature data. The corrected relative excitation spectra all show significant differences from the absorption spectrum when both are determined under identical conditions of concentration and spectral bandwidth on the same instrument. This behavior is discussed in terms J. of a) n-π and π-π states, b) emission from a minor tautomer and c) kinetics of competing deactivation processes. From a development of c), rate constants for processes occurring from the 0' level are calculated for intersystem crossing, internal conversion, and (singlet) photochemical reaction in thymine and uracil. From solvent induced shifts of absorption and fluorescence spectra, the excited state dipole moment of thymine μe = 8. 0 ± 2. 20) and the π-π* nature of the transitions responsible for fluorescence are deduced. Consideration of the singlet lifetime of thymine in relation to the rates of excited state process implies that fluorescence may occur from a Franck-Condon excited state and this is discussed in terms of possible solute-solvent interactions. A study of the pH effects on the spectral shape, magnitude, and polarization of thymine emission concludes that the emitting states of J. ionized thymine are of a π-π* nature , that fluorescence may arise within the 1, 2,4, 5, and 6 π- conjugated system in the thymine molecule, and that excited state ionizations do not occur in the fluorescent forms of thymine. These deductions are discussed in relation to DNA excited state processes. Advisors/Committee Members: Daniels, Malcolm (advisor).

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Hauswirth, W. W. (1970). Fluorescence studies on the bases of the nucleic acids in solution at room temperature. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46021

Chicago Manual of Style (16^th Edition):

Hauswirth, William Walter. “Fluorescence studies on the bases of the nucleic acids in solution at room temperature.” 1970. Doctoral Dissertation, Oregon State University. Accessed February 27, 2021. http://hdl.handle.net/1957/46021.

MLA Handbook (7^th Edition):

Hauswirth, William Walter. “Fluorescence studies on the bases of the nucleic acids in solution at room temperature.” 1970. Web. 27 Feb 2021.

Vancouver:

Hauswirth WW. Fluorescence studies on the bases of the nucleic acids in solution at room temperature. [Internet] [Doctoral dissertation]. Oregon State University; 1970. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1957/46021.

Council of Science Editors:

Hauswirth WW. Fluorescence studies on the bases of the nucleic acids in solution at room temperature. [Doctoral Dissertation]. Oregon State University; 1970. Available from: http://hdl.handle.net/1957/46021

University of Manitoba

10. Tavassoli, Marjan. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase).

Degree: Chemistry, 2013, University of Manitoba

URL: http://hdl.handle.net/1993/14918

► 1,6-Anhydro-N-acetylmuramic acid kinase (AnmK) is a homodimeric enzyme that can convert 1,6-Anhydro-N-acetylmuramic acid into N-acetylglucosamine-6-phosphate by consuming one molecule of ATP in murein recycling in… (more)

Subjects/Keywords: Fluorescence; AnmK

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APA (6^th Edition):

Tavassoli, M. (2013). Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase). (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/14918

Chicago Manual of Style (16^th Edition):

Tavassoli, Marjan. “Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase).” 2013. Masters Thesis, University of Manitoba. Accessed February 27, 2021. http://hdl.handle.net/1993/14918.

MLA Handbook (7^th Edition):

Tavassoli, Marjan. “Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase).” 2013. Web. 27 Feb 2021.

Vancouver:

Tavassoli M. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase). [Internet] [Masters thesis]. University of Manitoba; 2013. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1993/14918.

Council of Science Editors:

Tavassoli M. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase). [Masters Thesis]. University of Manitoba; 2013. Available from: http://hdl.handle.net/1993/14918

Montana State University

11. Tusell, Jose Ramon. Computation of tryptophan fluorescence quenching by amide and histidine.

Degree: PhD, College of Letters & Science, 2011, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/2458

► Tryptophan fluorescence quantum yield is widely used to follow protein folding for the villin headpiece subdomain (HP-35) and a synthetic peptide Ac-W-(A) ₃ -H +… (more)

▼ Tryptophan fluorescence quantum yield is widely used to follow protein folding for the villin headpiece subdomain (HP-35) and a synthetic peptide Ac-W-(A) ₃ -H + -NH ₂ (WH5). These biopolymers have a histidine residue, which is a potent quencher of tryptophan fluorescence, positioned four amino acids away from tryptophan. Experiments assumed that when folding occurs the fluorescence of tryptophan will be quenched by histidine due to the formation of an alpha helix. The reliability of folding and unfolding rate constants determined by tryptophan fluorescence has been called into question by several computational studies. A method to calculate the electron transfer matrix element was developed for different donor/acceptor systems. This method shows that the electron transfer matrix element is sensitive to orientation at close distances and that it does not follow a simple exponential decay with distance. This thesis improved the methods developed by Callis and coworker by conducting 100 ns long simulations for single tryptophan proteins and by modifying the calculation of the fluorescence quantum yield to account for heterogeneity in the calculated electron transfer rates. In addition the method was extended to calculate electron transfer rate constants for histidine quenching by conducting 1 microsecond long simulations of HP-35 and WH5. Calculated tryptophan fluorescence quantum yields for the single tryptophan proteins show better agreement with experiment than was previously reported. Simulations for HP-35 and WH5 indicate that the ability of histidine to quench the fluorescence of tryptophan is surprisingly controlled by the energy gap dependence on the distance that separates them. The energy gap dependence on this distance arises from water solvation around histidine. At large distances this solvation decreases the ability of histidine to accept an electron from tryptophan. Different tryptophan/histidine rotamers control this distance. Even when HP-35 is completely folded much of the time histidine does not quench tryptophan fluorescence contrary to the idea that histidine is only close when HP-35 is folded. The calculated fluorescence quantum yield is sensitive to the distribution of close and far conformations and the rate of exchange between these two conformations. This sensitivity gives credibility to the folding/unfolding rates derived from tryptophan fluorescence quantum yields. Advisors/Committee Members: Chairperson, Graduate Committee: Patrik R. Callis (advisor).

Subjects/Keywords: Fluorescence.; Tryptophan.

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APA (6^th Edition):

Tusell, J. R. (2011). Computation of tryptophan fluorescence quenching by amide and histidine. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/2458

Chicago Manual of Style (16^th Edition):

Tusell, Jose Ramon. “Computation of tryptophan fluorescence quenching by amide and histidine.” 2011. Doctoral Dissertation, Montana State University. Accessed February 27, 2021. https://scholarworks.montana.edu/xmlui/handle/1/2458.

MLA Handbook (7^th Edition):

Tusell, Jose Ramon. “Computation of tryptophan fluorescence quenching by amide and histidine.” 2011. Web. 27 Feb 2021.

Vancouver:

Tusell JR. Computation of tryptophan fluorescence quenching by amide and histidine. [Internet] [Doctoral dissertation]. Montana State University; 2011. [cited 2021 Feb 27]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2458.

Council of Science Editors:

Tusell JR. Computation of tryptophan fluorescence quenching by amide and histidine. [Doctoral Dissertation]. Montana State University; 2011. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2458

Kansas State University

12. Anderson, Richard. A comparison of intensities of indium and thallium sensitized fluorescene.

Degree: 1954, Kansas State University

URL: http://hdl.handle.net/2097/19298

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Anderson, R. (1954). A comparison of intensities of indium and thallium sensitized fluorescene. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/19298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Thesis, Kansas State University. Accessed February 27, 2021. http://hdl.handle.net/2097/19298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Web. 27 Feb 2021.

Vancouver:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Internet] [Thesis]. Kansas State University; 1954. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2097/19298.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Thesis]. Kansas State University; 1954. Available from: http://hdl.handle.net/2097/19298

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kansas State University

13. Anderson, Richard. A comparison of intensities of indium and thallium sensitized fluorescene.

Degree: 1954, Kansas State University

URL: http://hdl.handle.net/2097/35868

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Anderson, R. (1954). A comparison of intensities of indium and thallium sensitized fluorescene. (Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/35868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Thesis, Kansas State University. Accessed February 27, 2021. http://hdl.handle.net/2097/35868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anderson, Richard. “A comparison of intensities of indium and thallium sensitized fluorescene.” 1954. Web. 27 Feb 2021.

Vancouver:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Internet] [Thesis]. Kansas State University; 1954. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2097/35868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anderson R. A comparison of intensities of indium and thallium sensitized fluorescene. [Thesis]. Kansas State University; 1954. Available from: http://hdl.handle.net/2097/35868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Macquarie University

14. Deng, Wei. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.

Degree: PhD, 2011, Macquarie University

URL: http://hdl.handle.net/1959.14/220460

►

"A thesis submitted to Macquarie University for the degree of Doctor of Philosophy, Department of Physics and Engineering, April 2011"

Bibliography: p. 153-183.

1. Acknowledgements… (more)

▼

"A thesis submitted to Macquarie University for the degree of Doctor of Philosophy, Department of Physics and Engineering, April 2011"

Bibliography: p. 153-183.

1. Acknowledgements – 2. List of publications – 3. Abstract – 4. Introduction – 5. Fluorescence and fluorescence instrumentation (review) – 6. Metal-enhanced fluorescence and its biological application (review) – 7. Enhanced flow cytometry-based bead immunoassays using metal nanostructures – 8. Metal nanostructure enhanced fluorescence of europium chelate BHHCT-Eu3+ applied to bioassays and time-gated bioimaging – 9. Upconversion phosphor nanoparticles and its application in bioassays (review) – 10. Upconversion in NaYF4 : Yb, Er nanoparticles amplified by metal nanostructures – 11. Ultrabright Eu-doped plasmonic [email protected] nanostructures: time-gated bioprobes with single particle sensitivity and negligible background – 12. Conclusion.

Over the last two decades, fluorescence based detection has become one of the leading sensing technologies in biomedical, biological and related sciences. Its sensitivity makes it possible to detect a single biomolecule through labeling with a suitable fluorophore. Two principal fluorophore properties, brightness and photostability, are fundamentally important to achieve a high level of sensitivity and in many conventional fluorophores these often fall short of the requirements. Among the methods used to improve the sensitivity of fluorescence detection, the metal-enhanced fluorescence (MEF) technique has been recently actively developed. The MEF phenomenon occurs when an excited fluorophore is located in close proximity to metals, and it is particularly pronounced near noble metal nanostructures. Electrons in such metal nanostructures exhibit strong resonances often located in the visible part of the spectrum (also known as surface plasmon resonance). They can interact with proximal fluorophores modifying their optical properties and producing increased quantum yield (fluorescence efficiency) and improved photostability. It has been experimentally demonstrated that the MEF technique can increase fluorescence intensity up to several hundreds times. The work through my PhD project mainly focus on siver nanostructures and its potential in fluorescence-based applications requiring very high sensitivity because their strong surface plasmon resonance in the visible matches the absorption and emission bands of most fluorophores. I began with synthesis for Ag nanostructure-coated silica beads in the solution-based platform as a new MEF substrate. My first study employed these nanostructures to enhance the fluorescence readout on individual silica beads. These Ag nanostructure were deposited on micrometer size silica beads. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay and AlexaFluor 430 labeling. Approximately 8.5-fold and 10.1-fold higher fluorescence intensities at 430 nm excitation were, respectively, observed from silvered 400 nm and 5 μm silica beads deposited on…

Advisors/Committee Members: Macquarie University. Dept. of Physics and Engineering.

Subjects/Keywords: Fluorescence spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Deng, W. (2011). Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/220460

Chicago Manual of Style (16^th Edition):

Deng, Wei. “Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.” 2011. Doctoral Dissertation, Macquarie University. Accessed February 27, 2021. http://hdl.handle.net/1959.14/220460.

MLA Handbook (7^th Edition):

Deng, Wei. “Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.” 2011. Web. 27 Feb 2021.

Vancouver:

Deng W. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. [Internet] [Doctoral dissertation]. Macquarie University; 2011. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1959.14/220460.

Council of Science Editors:

Deng W. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. [Doctoral Dissertation]. Macquarie University; 2011. Available from: http://hdl.handle.net/1959.14/220460

University of Oklahoma

15. Gunawardana, Gunawardana Buddika. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/319531

We believe this simple solution based approach we have developed to produce bright photostable metal fluorophore aggregates would have a greater potential to be used in the development of ultra-bright fluorescent tags for cellular imaging. Advisors/Committee Members: Halterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence; Nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gunawardana, G. B. (2012). Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/319531

Chicago Manual of Style (16^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2021. http://hdl.handle.net/11244/319531.

MLA Handbook (7^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Web. 27 Feb 2021.

Vancouver:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/11244/319531.

Council of Science Editors:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/319531

University of Oklahoma

16. Gunawardana, Gunawardana Buddika. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/318577

We believe this simple solution based approach we have developed to produce bright photostable metal fluorophore aggregates would have a greater potential to be used in the development of ultra-bright fluorescent tags for cellular imaging. Advisors/Committee Members: Halterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence; Nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gunawardana, G. B. (2012). Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/318577

Chicago Manual of Style (16^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2021. http://hdl.handle.net/11244/318577.

MLA Handbook (7^th Edition):

Gunawardana, Gunawardana Buddika. “Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles.” 2012. Web. 27 Feb 2021.

Vancouver:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/11244/318577.

Council of Science Editors:

Gunawardana GB. Study of Metal-Enhanced Fluorescence of Dye-Doped Silica Nanoparticles. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/318577

University of Oklahoma

17. Green, Nathaniel Scott. Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates.

Degree: PhD, 2013, University of Oklahoma

URL: http://hdl.handle.net/11244/319182

► Construction and aggregation of functionalized silica and gold nano-materials provides an opportunity to advance the field of fluorescence. The synthesis of gold nano-particles allows control… (more)

Subjects/Keywords: Fluorescence; Nanoparticles

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APA (6^th Edition):

Green, N. S. (2013). Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/319182

Chicago Manual of Style (16^th Edition):

Green, Nathaniel Scott. “Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates.” 2013. Doctoral Dissertation, University of Oklahoma. Accessed February 27, 2021. http://hdl.handle.net/11244/319182.

MLA Handbook (7^th Edition):

Green, Nathaniel Scott. “Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates.” 2013. Web. 27 Feb 2021.

Vancouver:

Green NS. Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates. [Internet] [Doctoral dissertation]. University of Oklahoma; 2013. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/11244/319182.

Council of Science Editors:

Green NS. Plasmonic Properties and Enhanced Fluorescence of Gold and Dye-Doped Silica Nanoparticle Aggregates. [Doctoral Dissertation]. University of Oklahoma; 2013. Available from: http://hdl.handle.net/11244/319182

Michigan State University

18. Majchrowski, Thomas Francis. Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature.

Degree: MS, Dept. of Chemistry, 1971, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:11932

Subjects/Keywords: Fluorescence

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APA (6^th Edition):

Majchrowski, T. F. (1971). Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:11932

Chicago Manual of Style (16^th Edition):

Majchrowski, Thomas Francis. “Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature.” 1971. Masters Thesis, Michigan State University. Accessed February 27, 2021. http://etd.lib.msu.edu/islandora/object/etd:11932.

MLA Handbook (7^th Edition):

Majchrowski, Thomas Francis. “Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature.” 1971. Web. 27 Feb 2021.

Vancouver:

Majchrowski TF. Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature. [Internet] [Masters thesis]. Michigan State University; 1971. [cited 2021 Feb 27]. Available from: http://etd.lib.msu.edu/islandora/object/etd:11932.

Council of Science Editors:

Majchrowski TF. Molecular fluorescence quantum efficiency as a function of excitation wavelength, concentration, acidity and temperature. [Masters Thesis]. Michigan State University; 1971. Available from: http://etd.lib.msu.edu/islandora/object/etd:11932

Tulane University

19. Elfer, Katherine. The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology.

Degree: 2018, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:79052

►

Current microscopy-based tissue diagnostics, particularly hematoxylin and eosin (H&E) histology, requires multiple complex tissue processing steps: fixation, paraffin embedding, microtome sectioning, dying the tissue, and… (more)

▼

Current microscopy-based tissue diagnostics, particularly hematoxylin and eosin (H&E) histology, requires multiple complex tissue processing steps: fixation, paraffin embedding, microtome sectioning, dying the tissue, and imaging individual slides through a bright field microscope. The time and labor-intensive result of this process makes it unsuitable for patient point-of-care evaluation. Therefore, many bedside procedures are completed without efficient real-time analysis of tissue adequacy and diagnostic results are unnecessarily delayed. Additionally, research experiments that require information regarding changes to tissue morphology or function before proceeding to the next experimental phase are severely interrupted by histology processing in their workflow. Fluorescence histology, which relies on rapid fluorescent staining of tissue, optical sectioning microscopy, and image processing for digital viewing, can provide an inexpensive, non-destructive, 3-dimensional, and fast alternative to traditional histology and point-of-care screening protocols. The objective of this work is to further advance the concept of “fluorescence histology,” in which traditional histopathology preparation methods are replaced by optical-sectioning (in lieu of physical sectioning), sensitive and flexible fluorescence-based contrast (in lieu of chromophore-based contrast), and computational strategies to replicate traditional color-schemes. In this work, we demonstrate the development and use of a fluorescent analogue to H&E on fixed and frozen tissue sections and fresh human biopsies. This fluorescent analogue, DRAQ5 & eosin, is compared against the current single-agent, monochrome fluorescence histology system, and their effects on diagnostic downstream molecular analyses, including quantitative-PCR and immunohistochemistry, is evaluated. We create a methodology to develop and characterize fluorescent analogues for any histological stain, with demonstration using Masson’s Trichrome and Periodic Acid-Schiff, enabling the expansion of fluorescence histology for multiple applications. This work demonstrates the ability to improve point-of-care pathology and research by replacing destructive, incomplete, and time-consuming histology with fluorescence histology, which preserves the tissue for later analysis or experiments while providing accurate and rapid histology assessment.

1

Katherine Elfer

Advisors/Committee Members: Brown, Quincy (Thesis advisor), School of Science & Engineering Biomedical Engineering (Degree granting institution).

Subjects/Keywords: Fluorescence Histology; Fluorescence Microscopy; Image Processing

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APA (6^th Edition):

Elfer, K. (2018). The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:79052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elfer, Katherine. “The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology.” 2018. Thesis, Tulane University. Accessed February 27, 2021. https://digitallibrary.tulane.edu/islandora/object/tulane:79052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elfer, Katherine. “The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology.” 2018. Web. 27 Feb 2021.

Vancouver:

Elfer K. The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology. [Internet] [Thesis]. Tulane University; 2018. [cited 2021 Feb 27]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:79052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elfer K. The development and evaluation of a pseudo-histological staining and image processing system for use in point-of-care ex-vivo fluorescence histology. [Thesis]. Tulane University; 2018. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:79052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

20. Mlčoch, Tomáš. Fluorescence huminových kyselin 3D excitačně emisní metodou.: Fluorescence spectroscopy of humic acids by excitation-emission matrix.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/13879

► The aim of the diploma thesis was measurement and characterization of EEM (excitation emisssion spectra) of IHSS humic acids standards and soil, lignite humic and… (more)

Subjects/Keywords: fluorescence; huminové kyseliny; fluorescence; humic acids

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APA (6^th Edition):

Mlčoch, T. (2019). Fluorescence huminových kyselin 3D excitačně emisní metodou.: Fluorescence spectroscopy of humic acids by excitation-emission matrix. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/13879

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mlčoch, Tomáš. “Fluorescence huminových kyselin 3D excitačně emisní metodou.: Fluorescence spectroscopy of humic acids by excitation-emission matrix.” 2019. Thesis, Brno University of Technology. Accessed February 27, 2021. http://hdl.handle.net/11012/13879.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mlčoch, Tomáš. “Fluorescence huminových kyselin 3D excitačně emisní metodou.: Fluorescence spectroscopy of humic acids by excitation-emission matrix.” 2019. Web. 27 Feb 2021.

Vancouver:

Mlčoch T. Fluorescence huminových kyselin 3D excitačně emisní metodou.: Fluorescence spectroscopy of humic acids by excitation-emission matrix. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/11012/13879.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mlčoch T. Fluorescence huminových kyselin 3D excitačně emisní metodou.: Fluorescence spectroscopy of humic acids by excitation-emission matrix. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/13879

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Remy, Charlotte. Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules.

Degree: Docteur es, Chimie, 2016, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2016SACLN070

► La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les… (more)

▼ La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les résidus de pesticides tels que l’atrazine ainsi que la mélamine font notamment partie de ces molécules dangereuses pour la santé humaine. Ces deux molécules sont principalement dosées par des techniques lourdes et coûteuses comme la spectrométrie de masse, la chromatographie ou l’électrochimie. De même, la détection des amines biogéniques représente un intérêt sociétal. Elles sont produites par des bactéries durant la décarboxylation des acides aminés dans les cellules. Leur détection permet ainsi d’évaluer la contamination microbiologique et la dégradation potentielle d’un aliment. Elles sont aujourd’hui dosées par chromatographie en phase liquide ou en en phase gaz, par électrochromatographie capillaire et par spectroscopie UV-visible. Quelques exemples de détection par fluorescence ont déjà été décrits dans la littérature mais la nécessité de développer de nouveaux récepteurs fluorescents efficaces est bien réelle.La fluorescence est une technique qui offre de multiples avantages tels que la sensibilité, la sélectivité et un faible coût. De nombreuses sondes fluorescentes capables de détecter des métaux lourds ont été développées au laboratoire PPSM. Cependant, la détection de molécules neutres par fluorescence représente un défi supplémentaire en raison de la nature plus faible de l’interaction, comparée à celle entre espèces chargées.La première étape de cette thèse a été de concevoir et de synthétiser un ensemble de sondes moléculaires fluorescentes, aussi bien pour la détection de l’atrazine, de ses produits de dégradation et des dérivés de la mélamine que pour la détection des amines biogéniques. Des fluorophores dérivés de la molécule de maléimide, de naphthalimide et de l’acide barbiturique ont ainsi été développés pour sonder les dérivés de triazine en exploitant leur système de trois liaisons hydrogène pour la reconnaissance moléculaire. De même, un calix[6]arène fluorescent a été conçu pour déceler la présence des amines biogéniques qui provoqueront une réponse fluorescente par encapsulation dans le calixarène.La deuxième étape a consisté à étudier les propriétés photophysiques de ces sondes. La sonde Naphth-AlcyneOMe possède un rendement quantique élevé, s’est révélée fortement solvatochrome. Elle est de plus sensible à la déprotonation de sa fonction imide. Des études RMN et de modélisation moléculaire ont également été menées afin de caractériser les sondes de manière plus approfondie et de comprendre plus précisément leur réactivité. La spectroscopie RMN a confirmé l’interaction par liaison hydrogène entre les sondes maléimide et naphthalimides et la molécule d’atrazine. Elle a aussi mis en évidence l’encapsulation de l’heptylamine dans le calix[6]arène. Pour sa part, la modélisation moléculaire nous a permis de mieux comprendre la photophysique de la sonde Naphth-TriazoleOMe.Enfin, la capacité des sondes à détecter les divers… Advisors/Committee Members: Leray, Isabelle (thesis director).

Subjects/Keywords: Synthèse; Fluorescence; Sonde; Synthesis; Fluorescence; Probe

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Remy, C. (2016). Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLN070

Chicago Manual of Style (16^th Edition):

Remy, Charlotte. “Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed February 27, 2021. http://www.theses.fr/2016SACLN070.

MLA Handbook (7^th Edition):

Remy, Charlotte. “Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules.” 2016. Web. 27 Feb 2021.

Vancouver:

Remy C. Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Feb 27]. Available from: http://www.theses.fr/2016SACLN070.

Council of Science Editors:

Remy C. Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres : Synthesis and spectroscopic studies of fluorescent probes for the detection of neutral molecules. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLN070

University of Pennsylvania

22. Chiorazzo, Michael. Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer.

Degree: 2016, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1653

► Breast cancer is the most commonly diagnosed cancer in women and the number two cause of cancer associated death. Surgery is the first line of… (more)

▼ Breast cancer is the most commonly diagnosed cancer in women and the number two cause of cancer associated death. Surgery is the first line of defense in treating breast cancer and the success of surgery has been shown to predict the probability of cancer reoccurrence. Near Infrared (NIR) fluorescence guided surgery offers the potential to increase the accuracy of surgical resection by the use of fluorescence imaging agents targeted towards cancer. Cytosolic Phospholipase A2 (cPLA2) activity is enhanced in triple negative breast cancer and is associated with resistance to therapy in luminal-like cancers. The goal of this work is to develop activatable fluorophores targeted towards cPLA2 and evaluate their use for the molecular imaging of breast cancer in order to determine their potential as contrast agents for NIR fluorescence guided surgery. A series of tricyclic fluorophores were caged through esterification with fatty acids. Arachidonic acid was utilized to generate cPLA2 selective probes and palmitic acid was used to generate control probes. In vitro, probes were shown to activate in the presence of purified PLA2 and results were used to identify the most promising probe: DDAO arachidonate. Cellular fluorescence spectroscopy and microscopy showed DDAO arachidonate activated in breast cancer cell lines expressing cPLA2, where control probe did not. Modulation of cPLA2 activity through inhibition, induction, and genetic knockdown, resulted in corresponding changes in fluorescence generation. In a panel of breast cancer cell lines, cPLA2 expression, varied based on molecular subtype and correlated with DDAO arachidonate activation. Finally, DDAO arachidonate, when painted onto human triple negative tumor mouse xenografts, resulted in selective activation of tumor but not surrounding healthy tissue. Together, these results indicate DDAO arachidonate is a useful agent for measuring cPLA2 activity in both breast cancer cells and tumors and may offer the potential to aid in the fluorescence guided surgery of triple negative breast cancer.

Subjects/Keywords: Activatable Fluorescence; Breast Cancer; Fluorescence Guided Surgery

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APA (6^th Edition):

Chiorazzo, M. (2016). Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chiorazzo, Michael. “Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer.” 2016. Thesis, University of Pennsylvania. Accessed February 27, 2021. https://repository.upenn.edu/edissertations/1653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chiorazzo, Michael. “Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer.” 2016. Web. 27 Feb 2021.

Vancouver:

Chiorazzo M. Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer. [Internet] [Thesis]. University of Pennsylvania; 2016. [cited 2021 Feb 27]. Available from: https://repository.upenn.edu/edissertations/1653.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chiorazzo M. Activatable Fluorophores for the Molecular Imaging of Cytosolic Phospholipase A2 in Breast Cancer. [Thesis]. University of Pennsylvania; 2016. Available from: https://repository.upenn.edu/edissertations/1653

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

23. Chen, Yu-wei. Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes.

Degree: PhD, Chemistry, 2009, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557

► To reduce the image blurring which originates from contributions of a cylindrical array of photolysis events in a photo-induced experiment, a variant of fluorescence imaging… (more)

Subjects/Keywords: Sliced fluorescence imaging

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APA (6^th Edition):

Chen, Y. (2009). Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557

Chicago Manual of Style (16^th Edition):

Chen, Yu-wei. “Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes.” 2009. Doctoral Dissertation, NSYSU. Accessed February 27, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557.

MLA Handbook (7^th Edition):

Chen, Yu-wei. “Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes.” 2009. Web. 27 Feb 2021.

Vancouver:

Chen Y. Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes. [Internet] [Doctoral dissertation]. NSYSU; 2009. [cited 2021 Feb 27]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557.

Council of Science Editors:

Chen Y. Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes. [Doctoral Dissertation]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557

University of Alberta

24. Assempour, Nazanin. Tuning the properties of protein-based biosensors by chromophore replacement strategies.

Degree: MS, Department of Chemistry, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/cxg94hp74q

► Optical imaging of membrane potentials has become an immensely popular approach to study neuronal activity. Over the past decades, a number of voltage indicators have… (more)

Subjects/Keywords: Fluorescence; Biosensor; Chromophore

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APA (6^th Edition):

Assempour, N. (2014). Tuning the properties of protein-based biosensors by chromophore replacement strategies. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cxg94hp74q

Chicago Manual of Style (16^th Edition):

Assempour, Nazanin. “Tuning the properties of protein-based biosensors by chromophore replacement strategies.” 2014. Masters Thesis, University of Alberta. Accessed February 27, 2021. https://era.library.ualberta.ca/files/cxg94hp74q.

MLA Handbook (7^th Edition):

Assempour, Nazanin. “Tuning the properties of protein-based biosensors by chromophore replacement strategies.” 2014. Web. 27 Feb 2021.

Vancouver:

Assempour N. Tuning the properties of protein-based biosensors by chromophore replacement strategies. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2021 Feb 27]. Available from: https://era.library.ualberta.ca/files/cxg94hp74q.

Council of Science Editors:

Assempour N. Tuning the properties of protein-based biosensors by chromophore replacement strategies. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cxg94hp74q

Cornell University

25. Andoy, Nesha May. Single-Molecule Fluorescence Studies Of Metalloregulators, Metalloenzymes, And Nanocatalysts.

Degree: PhD, Chemistry and Chemical Biology, 2011, Cornell University

URL: http://hdl.handle.net/1813/33590

► This work presents the development and application of single-molecule fluorescence-based methodologies to study bio-inorganic systems and catalysis of nanostructured materials. Single-molecule fluorescence microscopy was used… (more)

▼ This work presents the development and application of single-molecule fluorescence-based methodologies to study bio-inorganic systems and catalysis of nanostructured materials. Single-molecule fluorescence microscopy was used to examine: (i) protein-DNA interactions using single-molecule fluorescence resonance energy transfer (smFRET), (ii) single-molecule enzymology of tyrosinase using fluorescence quenching via energy transfer, and (iii) structure-activity correlation of gold nanoparticle catalysis using super-resolution imaging. Engineered DNA Holliday junction (HJ) was used as the smFRET reporter for interactions of a metalloregulator, CueR, with DNA for transcriptional regulation. Both the apo- and holo-forms of CueR interact with and change the structures and dynamics of interconversion between the two conformations of the HJ. Singlemolecule kinetic analyses revealed differences between apo- and holo- CueR in their interactions with DNA. These changes observed on HJ upon CueR binding reflect the protein actions on DNA for regulating gene transcription. The single-molecule enzymology of tyrosinase was also investigated. Catalysis coupled fluorescence quenching (CCFQ) was used to monitor the state of the binuclear-copper active site of the enzyme. The intermediates of this metal center exhibit different absorption features as the enzyme goes through the catalytic cycle. Using a fluorescent probe whose fluorescence spectrum overlaps with the absorption spectrum of one of the intermediates of the active site, the state of the active site of the enzyme was directly monitored. The structure-activity correlation of single nanoparticle catalysts was achieved by applying super-resolution fluorescence imaging on single-particle catalysis. The generation of a fluorescent product from a non-fluorescent reactant on the surface of nanoparticles was imaged using single-molecule fluorescence microscopy. Precise nanometer localization of where the products are generated, with a resolution of ~25 nm, was obtained by fitting the point spread function of the emission of a product molecule with a two-dimensional Gaussian function. By taking the SEM image of the same nanoparticles and overlaying them to those obtained from super-resolution imaging, direct correlation of the activity and structure of nanocatalysts was achieved at the single-particle level. Advisors/Committee Members: Chen, Peng (chair), Marohn, John A. (committee member), Baird, Barbara Ann (committee member).

Subjects/Keywords: single-molecule fluorescence

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APA (6^th Edition):

Andoy, N. M. (2011). Single-Molecule Fluorescence Studies Of Metalloregulators, Metalloenzymes, And Nanocatalysts. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/33590

Chicago Manual of Style (16^th Edition):

Andoy, Nesha May. “Single-Molecule Fluorescence Studies Of Metalloregulators, Metalloenzymes, And Nanocatalysts.” 2011. Doctoral Dissertation, Cornell University. Accessed February 27, 2021. http://hdl.handle.net/1813/33590.

MLA Handbook (7^th Edition):

Andoy, Nesha May. “Single-Molecule Fluorescence Studies Of Metalloregulators, Metalloenzymes, And Nanocatalysts.” 2011. Web. 27 Feb 2021.

Vancouver:

Andoy NM. Single-Molecule Fluorescence Studies Of Metalloregulators, Metalloenzymes, And Nanocatalysts. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1813/33590.

Council of Science Editors:

Andoy NM. Single-Molecule Fluorescence Studies Of Metalloregulators, Metalloenzymes, And Nanocatalysts. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/33590

Anna University

26. Rajasekaran R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.

Degree: Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy, 2015, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/33585

►

Cancer is a dreaded disease and one of the leading causes of death newlineWorldwide The complete treatment of cancer is possible only when it is… (more)

Subjects/Keywords: Fluorescence spectroscopy; Worldwide

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APA (6^th Edition):

R, R. (2015). Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/33585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

R, Rajasekaran. “Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.” 2015. Thesis, Anna University. Accessed February 27, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/33585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

R, Rajasekaran. “Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.” 2015. Web. 27 Feb 2021.

Vancouver:

R R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. [Internet] [Thesis]. Anna University; 2015. [cited 2021 Feb 27]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

R R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. [Thesis]. Anna University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McMaster University

27. Yeh, Shu-Chi. Fluorescence of PDT Photosensitizers for Quantitative Cancer Diagnosis and Treatment Monitoring.

Degree: PhD, 2015, McMaster University

URL: http://hdl.handle.net/11375/16735

►

Photodynamic therapy (PDT) has been considered a favorable approach in certain oncology applications for its little invasiveness and better targeting specificity compared to conventional therapies.… (more)

▼

Photodynamic therapy (PDT) has been considered a favorable approach in certain oncology applications for its little invasiveness and better targeting specificity compared to conventional therapies. In PDT, localized photosensitizers can be activated by light to produce cytotoxic oxygen species. However, the prescribed drug and light doses do not provide satisfying outcomes as the PDT efficacy relies strongly on the interplay between localized dose factors. Therefore, the fluorescence emission from active photosensitizers has been investigated extensively for real-time PDT dosimetry. This dissertation focuses on characterizing fluorescence properties of two photosensitizers, Photofrin® and PpIX, in cellular models and discusses about their potential clinical applications. First, we introduc time-resolve fluorescence (TRF) of photosensitizers as a potential tool in PDT dosimetry. TRF acquires fluorescence decay profiles and it is sensitive to drug-microenvironment interactions that occur frequently in PDT. Therefore, it provides complementary information in addition to fluorescence spectra that could be subject to intensity artifacts. In this dissertation, we review TRF studies on PDT photosensitizers, and quantify TRF parameters of Photofrin® at various subcellular locations. Moreover, analytical solutions are developed to correct distorted TRF measurements from commonly used time-domain data acquisition. Second, we report a new concept – integrated detection and treatment of Barrett’s Esophagus (BE). BE is a pre-cancerous lesion considered as a major risk factor in developing esophageal cancers. However, early intervention of BE has remained a challenging issue as tissue biopsy introduces significant sampling errors and the separate procedures between diagnosis and treatment add relocation errors. We proposed to use PpIX fluorescence to highlight morphological features at the cellular level for quantitative classification, followed by well-characterized treatment. Current proof-of-concept studies were performed separately, whilst the detection and treatment can be integrated using confocal endomicroscopy technology. Overall, these studies examine the potential benefits provided by fluorescence of photosensitizers for cancer diagnosis and treatment monitoring.

Thesis

Doctor of Philosophy (PhD)

Advisors/Committee Members: Fang, Qiyin, Biomedical Engineering.

Subjects/Keywords: Fluorescence; Photodynamic therapy

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APA (6^th Edition):

Yeh, S. (2015). Fluorescence of PDT Photosensitizers for Quantitative Cancer Diagnosis and Treatment Monitoring. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16735

Chicago Manual of Style (16^th Edition):

Yeh, Shu-Chi. “Fluorescence of PDT Photosensitizers for Quantitative Cancer Diagnosis and Treatment Monitoring.” 2015. Doctoral Dissertation, McMaster University. Accessed February 27, 2021. http://hdl.handle.net/11375/16735.

MLA Handbook (7^th Edition):

Yeh, Shu-Chi. “Fluorescence of PDT Photosensitizers for Quantitative Cancer Diagnosis and Treatment Monitoring.” 2015. Web. 27 Feb 2021.

Vancouver:

Yeh S. Fluorescence of PDT Photosensitizers for Quantitative Cancer Diagnosis and Treatment Monitoring. [Internet] [Doctoral dissertation]. McMaster University; 2015. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/11375/16735.

Council of Science Editors:

Yeh S. Fluorescence of PDT Photosensitizers for Quantitative Cancer Diagnosis and Treatment Monitoring. [Doctoral Dissertation]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/16735

28. Barnett, Lauren Marie. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.

Degree: PhD, College of Letters & Science, 2017, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/12752

► To see the activity of large, integrated neural circuits functioning in real-time inside of a living brain, neuroscientists will need multiple genetically-encoded fluorescent activity sensors… (more)

Subjects/Keywords: Biosensors.; Brain.; Fluorescence.

11 more images…

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APA (6^th Edition):

Barnett, L. M. (2017). If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/12752

Chicago Manual of Style (16^th Edition):

Barnett, Lauren Marie. “If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.” 2017. Doctoral Dissertation, Montana State University. Accessed February 27, 2021. https://scholarworks.montana.edu/xmlui/handle/1/12752.

MLA Handbook (7^th Edition):

Barnett, Lauren Marie. “If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.” 2017. Web. 27 Feb 2021.

Vancouver:

Barnett LM. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. [Internet] [Doctoral dissertation]. Montana State University; 2017. [cited 2021 Feb 27]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/12752.

Council of Science Editors:

Barnett LM. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. [Doctoral Dissertation]. Montana State University; 2017. Available from: https://scholarworks.montana.edu/xmlui/handle/1/12752

University of Waterloo

29. Frasca, Franklin. Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence.

Degree: 2020, University of Waterloo

URL: http://hdl.handle.net/10012/16575

► Methods are introduced to characterize the products resulting from the coupling of a polyisobutylene terminated at one end with a succinic anhydride (PIBSA) and a… (more)

▼ Methods are introduced to characterize the products resulting from the coupling of a polyisobutylene terminated at one end with a succinic anhydride (PIBSA) and a polyamine to generate PIBSI products where the polyamine is flanked by one or two PIB segments via a succinimide bond. The molar fraction of doubly modified PIBSA (PIBSA2) molecules in two PIBSA samples (PIBSA-1 and PIBSA-2) was first determined using pyrene excimer formation (PEF) to analyse both PIBSA samples after labeling fractionated and unfractionated PIBSA samples with 1-pyrenemethylamine (PyMA) to generate PIBSA-PyMA. The ratios of succinic anhydrides, succinimides, or pyrenyl labels to the number of isobutylene units NSA/NIB, NSI/NIB, and NPy/NIB determined by FTIR and UV-Vis spectroscopy established that the succinic content of the unfractionated PIBSA-PyMA samples was roughly double that of the fractionated samples, suggesting a difference in the molecular weight distribution (MWD) of the samples which was confirmed by GPC analysis. Model free analysis (MFA) of the time-resolved fluorescence (TRF) decays yielded the average rate constant of pyrene excimer formation by diffusion (<k>) which increased linearly with [Py], and the molar fraction fagg of PyMA attached on PIBSA2 forming excimer by direct excitation of aggregated pyrene dimers, and in turn the fraction (f¬doubly) of PIBSA2 in each sample. fagg was higher for PIBSA-1 and did not change between the fractionated or unfractionated samples suggesting that PIBSA2 was evenly distributed across the MWD of PIBSA. The number average degree of polymerization (Xn) was calculated for the PIBSA-PyMAs and showed a linear relationship with the rate constant (kdiff) describing PEF by diffusion. Next, it was hypothesized that the composition of the mixture of a coupling reaction between PIBSA and a polyamine could be predicted with the knowledge of the ratio of the number of amines to SA units (NAm/NSA) available for the coupling reaction, the NSA/NIB ratio, and the fraction (fdoubly) of PIBSA2 chains in the PIBSA sample. Two programs were written to simulate the products generated from the coupling of PIBSA with a diamine to form either bis-PIBSI (b-PIBSI) and higher-order PIBSI oligomers, or to only form b-PIBSI assuming that steric hindrance prevented more than one PIBSA from linking to another PIBSA molecule. A set of PIBSA to hexamethylene diamine (HMDA) coupling reactions were conducted with a fractionated PIBSA-2 sample to yield the PIBSA-2-H(X) samples, where X equals the NAm/NSA ratio used in a coupling reaction, and generate DRI traces with GPC for use with the simulated data and the validation of the simulation programs. Parameterization of the DRI traces with sums of Gaussians enabled the determination of the fractions of PIBSA-like molecules (PLMs) and non-PIBSA-like molecules (non-PLMs) and their comparison with the fractions obtained from the simulations. This along with GPC analysis suggested that PIBSA-2 contained 30 wt% of unmaleated PIB. When assuming that the PIBSA coupling reaction…

Subjects/Keywords: polymers; fluorescence; dispersants

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APA (6^th Edition):

Frasca, F. (2020). Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/16575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Frasca, Franklin. “Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence.” 2020. Thesis, University of Waterloo. Accessed February 27, 2021. http://hdl.handle.net/10012/16575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Frasca, Franklin. “Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence.” 2020. Web. 27 Feb 2021.

Vancouver:

Frasca F. Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence. [Internet] [Thesis]. University of Waterloo; 2020. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10012/16575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Frasca F. Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence. [Thesis]. University of Waterloo; 2020. Available from: http://hdl.handle.net/10012/16575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Tran, Lan. Improving Photophysical Properties of Brilliant Violet(TM) Polymers via Structrual Modifications .

Degree: 2013, California State University – San Marcos

URL: http://hdl.handle.net/10211.8/431

► Improving Photophysical Properties of Brilliant Violet??? Polymers via Structural Modifications Sirigen/BDB Lan Tran May 2013 Professional Masters Degree Program Cal State University, San Marcos Department… (more)

Subjects/Keywords: polymers; fluorescence; photostability

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APA (6^th Edition):

Tran, L. (2013). Improving Photophysical Properties of Brilliant Violet(TM) Polymers via Structrual Modifications . (Thesis). California State University – San Marcos. Retrieved from http://hdl.handle.net/10211.8/431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tran, Lan. “Improving Photophysical Properties of Brilliant Violet(TM) Polymers via Structrual Modifications .” 2013. Thesis, California State University – San Marcos. Accessed February 27, 2021. http://hdl.handle.net/10211.8/431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tran, Lan. “Improving Photophysical Properties of Brilliant Violet(TM) Polymers via Structrual Modifications .” 2013. Web. 27 Feb 2021.

Vancouver:

Tran L. Improving Photophysical Properties of Brilliant Violet(TM) Polymers via Structrual Modifications . [Internet] [Thesis]. California State University – San Marcos; 2013. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10211.8/431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tran L. Improving Photophysical Properties of Brilliant Violet(TM) Polymers via Structrual Modifications . [Thesis]. California State University – San Marcos; 2013. Available from: http://hdl.handle.net/10211.8/431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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