subject:(Fluorescence spectroscopy)

University of Johannesburg

1. Freese, Margaret Helene. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.

Degree: 2015, University of Johannesburg

URL: http://hdl.handle.net/10210/14701

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M.Sc. (Chemistry)

The aim of this investigation was to study, and if possible, to correct curvature of calibration lines when solutions are analysed by X-ray… (more)

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Freese, M. H. (2015). Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/14701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freese, Margaret Helene. “Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.” 2015. Thesis, University of Johannesburg. Accessed March 04, 2021. http://hdl.handle.net/10210/14701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freese, Margaret Helene. “Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry.” 2015. Web. 04 Mar 2021.

Vancouver:

Freese MH. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. [Internet] [Thesis]. University of Johannesburg; 2015. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10210/14701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freese MH. Study of calibration line curvature in the analysis of solutions by X-ray fluorescence specrrometry. [Thesis]. University of Johannesburg; 2015. Available from: http://hdl.handle.net/10210/14701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Macquarie University

2. Deng, Wei. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.

Degree: PhD, 2011, Macquarie University

URL: http://hdl.handle.net/1959.14/220460

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"A thesis submitted to Macquarie University for the degree of Doctor of Philosophy, Department of Physics and Engineering, April 2011"

Bibliography: p. 153-183.

1. Acknowledgements… (more)

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"A thesis submitted to Macquarie University for the degree of Doctor of Philosophy, Department of Physics and Engineering, April 2011"

Bibliography: p. 153-183.

1. Acknowledgements – 2. List of publications – 3. Abstract – 4. Introduction – 5. Fluorescence and fluorescence instrumentation (review) – 6. Metal-enhanced fluorescence and its biological application (review) – 7. Enhanced flow cytometry-based bead immunoassays using metal nanostructures – 8. Metal nanostructure enhanced fluorescence of europium chelate BHHCT-Eu3+ applied to bioassays and time-gated bioimaging – 9. Upconversion phosphor nanoparticles and its application in bioassays (review) – 10. Upconversion in NaYF4 : Yb, Er nanoparticles amplified by metal nanostructures – 11. Ultrabright Eu-doped plasmonic [email protected] nanostructures: time-gated bioprobes with single particle sensitivity and negligible background – 12. Conclusion.

Over the last two decades, fluorescence based detection has become one of the leading sensing technologies in biomedical, biological and related sciences. Its sensitivity makes it possible to detect a single biomolecule through labeling with a suitable fluorophore. Two principal fluorophore properties, brightness and photostability, are fundamentally important to achieve a high level of sensitivity and in many conventional fluorophores these often fall short of the requirements. Among the methods used to improve the sensitivity of fluorescence detection, the metal-enhanced fluorescence (MEF) technique has been recently actively developed. The MEF phenomenon occurs when an excited fluorophore is located in close proximity to metals, and it is particularly pronounced near noble metal nanostructures. Electrons in such metal nanostructures exhibit strong resonances often located in the visible part of the spectrum (also known as surface plasmon resonance). They can interact with proximal fluorophores modifying their optical properties and producing increased quantum yield (fluorescence efficiency) and improved photostability. It has been experimentally demonstrated that the MEF technique can increase fluorescence intensity up to several hundreds times. The work through my PhD project mainly focus on siver nanostructures and its potential in fluorescence-based applications requiring very high sensitivity because their strong surface plasmon resonance in the visible matches the absorption and emission bands of most fluorophores. I began with synthesis for Ag nanostructure-coated silica beads in the solution-based platform as a new MEF substrate. My first study employed these nanostructures to enhance the fluorescence readout on individual silica beads. These Ag nanostructure were deposited on micrometer size silica beads. The fluorescence enhancement was investigated using a model AlexaFluor 430 IgG immunoassay and AlexaFluor 430 labeling. Approximately 8.5-fold and 10.1-fold higher fluorescence intensities at 430 nm excitation were, respectively, observed from silvered 400 nm and 5 μm silica beads deposited on…

Advisors/Committee Members: Macquarie University. Dept. of Physics and Engineering.

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Deng, W. (2011). Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/220460

Chicago Manual of Style (16^th Edition):

Deng, Wei. “Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.” 2011. Doctoral Dissertation, Macquarie University. Accessed March 04, 2021. http://hdl.handle.net/1959.14/220460.

MLA Handbook (7^th Edition):

Deng, Wei. “Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging.” 2011. Web. 04 Mar 2021.

Vancouver:

Deng W. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. [Internet] [Doctoral dissertation]. Macquarie University; 2011. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1959.14/220460.

Council of Science Editors:

Deng W. Metal nanostructure-enhanced fluorescence and its applications in bioassays and bioimaging. [Doctoral Dissertation]. Macquarie University; 2011. Available from: http://hdl.handle.net/1959.14/220460

University of Oklahoma

3. Singh, Anuradha. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.

Degree: PhD, 2012, University of Oklahoma

URL: http://hdl.handle.net/11244/318880

In conclusion, investigations were carried out for the development of next generation optoelectronic devices and novel real world applicable biosensors. Advisors/Committee Members: Hlaterman, Ronald L (advisor).

Subjects/Keywords: Fluorescence spectroscopy; Fluorescence microscopy; Biosensors

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APA (6^th Edition):

Singh, A. (2012). CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/318880

Chicago Manual of Style (16^th Edition):

Singh, Anuradha. “CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.” 2012. Doctoral Dissertation, University of Oklahoma. Accessed March 04, 2021. http://hdl.handle.net/11244/318880.

MLA Handbook (7^th Edition):

Singh, Anuradha. “CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH.” 2012. Web. 04 Mar 2021.

Vancouver:

Singh A. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. [Internet] [Doctoral dissertation]. University of Oklahoma; 2012. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/11244/318880.

Council of Science Editors:

Singh A. CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH. [Doctoral Dissertation]. University of Oklahoma; 2012. Available from: http://hdl.handle.net/11244/318880

Anna University

4. Rajasekaran R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.

Degree: Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy, 2015, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/33585

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Cancer is a dreaded disease and one of the leading causes of death newlineWorldwide The complete treatment of cancer is possible only when it is… (more)

Subjects/Keywords: Fluorescence spectroscopy; Worldwide

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APA (6^th Edition):

R, R. (2015). Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/33585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

R, Rajasekaran. “Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.” 2015. Thesis, Anna University. Accessed March 04, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/33585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

R, Rajasekaran. “Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;.” 2015. Web. 04 Mar 2021.

Vancouver:

R R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. [Internet] [Thesis]. Anna University; 2015. [cited 2021 Mar 04]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

R R. Discrimination of cancer from Normal subjects a study on the Characterization of urinary Metabolites using native Fluorescence spectroscopy;. [Thesis]. Anna University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oxford

5. Nolan, Rory. Algorithms for the correction of photobleaching.

Degree: PhD, 2018, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417

► The measured intensity (ideally in units of photon counts) of a fluorescent sample over time constitutes a time-series called an intensity trace. The idea of… (more)

Subjects/Keywords: Fluorescence correlation spectroscopy; Fluorescence; HIV; Image Analysis; Microscopy; Fluorescence fluctuation spectroscopy; Spectroscopy

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APA (6^th Edition):

Nolan, R. (2018). Algorithms for the correction of photobleaching. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417

Chicago Manual of Style (16^th Edition):

Nolan, Rory. “Algorithms for the correction of photobleaching.” 2018. Doctoral Dissertation, University of Oxford. Accessed March 04, 2021. http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417.

MLA Handbook (7^th Edition):

Nolan, Rory. “Algorithms for the correction of photobleaching.” 2018. Web. 04 Mar 2021.

Vancouver:

Nolan R. Algorithms for the correction of photobleaching. [Internet] [Doctoral dissertation]. University of Oxford; 2018. [cited 2021 Mar 04]. Available from: http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417.

Council of Science Editors:

Nolan R. Algorithms for the correction of photobleaching. [Doctoral Dissertation]. University of Oxford; 2018. Available from: http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417

Oregon State University

6. Pesklak, William Charles. Laser induced nonresonance atomic fluorescence observations in an analytical laser microprobe plume.

Degree: MS, Chemistry, 1984, Oregon State University

URL: http://hdl.handle.net/1957/40381

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Pesklak, W. C. (1984). Laser induced nonresonance atomic fluorescence observations in an analytical laser microprobe plume. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/40381

Chicago Manual of Style (16^th Edition):

Pesklak, William Charles. “Laser induced nonresonance atomic fluorescence observations in an analytical laser microprobe plume.” 1984. Masters Thesis, Oregon State University. Accessed March 04, 2021. http://hdl.handle.net/1957/40381.

MLA Handbook (7^th Edition):

Pesklak, William Charles. “Laser induced nonresonance atomic fluorescence observations in an analytical laser microprobe plume.” 1984. Web. 04 Mar 2021.

Vancouver:

Pesklak WC. Laser induced nonresonance atomic fluorescence observations in an analytical laser microprobe plume. [Internet] [Masters thesis]. Oregon State University; 1984. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1957/40381.

Council of Science Editors:

Pesklak WC. Laser induced nonresonance atomic fluorescence observations in an analytical laser microprobe plume. [Masters Thesis]. Oregon State University; 1984. Available from: http://hdl.handle.net/1957/40381

Oregon State University

7. Brown, D. W. (David W.), 1937-. Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy.

Degree: PhD, Biochemistry and Biophysics, 1992, Oregon State University

URL: http://hdl.handle.net/1957/36229

► Time-resolved fluorescence spectroscopy was used to monitor the effects of varying ionic strength on nucleosome core particle structure. Two main methods were used in these… (more)

▼ Time-resolved fluorescence spectroscopy was used to monitor the effects of varying ionic strength on nucleosome core particle structure. Two main methods were used in these studies. First, the fluorescence anisotropy decay of bound ethidium was measured and was shown to reflect the rotational tumbling of the core particle through solution, the longest recovered decay time being a measure of the rotational correlation time of the particle. A rotational correlation time of 165 ns was recovered for the native core particle at 10 mM ionic strength, in excellent agreement with that predicted by hydrodynamic calculations based on the particle's known size and shape. This technique was then used to measure the rotational correlation time of the core particle as a function of ionic strength. Below 1 mM salt the recovered rotational correlation times suggested little change in shape throughout the region of the reversible low salt transition. At very low ionic strengths (below 0.2 mM), where the low salt transition becomes irreversible, the rotational correlation time increased sharply to ~330 ns, suggesting a major change in the core particle structure. Computer modeling was performed to show that this increase was most likely due to a substantial elongation in the core particle structure, to at least a 5:1 axial ratio. At elevated ionic strengths, the rotational correlation time was seen to increase from the initial value of ~165 ns to ~240 ns as the salt concentration was raised from 10 mM to 0.35 M, with further increases being observed only above 0.65 M; we term this initial increase the moderate salt transition. Trypsinization of the core particles to remove the Nterminal histone domains completely abolished the increase, demonstrating that the moderate salt transition as measured by this technique involves the release of these protein domains from the body of the core particle. The second method used involved the measurement of the fluorescence decay of the intrinsic tyrosine residues of the core particle. This decay proved to be very complex, and was best represented by a distribution of lifetimes, suggesting different environments for the tyrosines. This distribution changed as the ionic strength of the solution changed, suggesting the movement of tyrosine residues to differing environments as the particle undergoes the low and moderate salt transitions, as well as the high salt dissociation. Advisors/Committee Members: Small, Enoch W. (advisor).

Subjects/Keywords: Fluorescence spectroscopy

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APA (6^th Edition):

Brown, D. W. (David W.), 1. (1992). Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/36229

Chicago Manual of Style (16^th Edition):

Brown, D. W. (David W.), 1937-. “Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy.” 1992. Doctoral Dissertation, Oregon State University. Accessed March 04, 2021. http://hdl.handle.net/1957/36229.

MLA Handbook (7^th Edition):

Brown, D. W. (David W.), 1937-. “Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy.” 1992. Web. 04 Mar 2021.

Vancouver:

Brown, D. W. (David W.) 1. Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy. [Internet] [Doctoral dissertation]. Oregon State University; 1992. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1957/36229.

Council of Science Editors:

Brown, D. W. (David W.) 1. Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy. [Doctoral Dissertation]. Oregon State University; 1992. Available from: http://hdl.handle.net/1957/36229

Hong Kong University of Science and Technology

8. Engels, Jan Frederik. Design and fabrication of novel fluorescent nanoparticles for signal generation in immunoassay.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-87403 ; https://doi.org/10.14711/thesis-b1626126 ; http://repository.ust.hk/ir/bitstream/1783.1-87403/1/th_redirect.html

► One of the major disadvantages of common Fluorescent Immunoassays is the Fluorophore per Protein ratio. In the normal settings several fluorophores such as FITC are… (more)

Subjects/Keywords: Nanoparticles ; Fluorescence spectroscopy ; Immunoassay

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APA (6^th Edition):

Engels, J. F. (2016). Design and fabrication of novel fluorescent nanoparticles for signal generation in immunoassay. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-87403 ; https://doi.org/10.14711/thesis-b1626126 ; http://repository.ust.hk/ir/bitstream/1783.1-87403/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Engels, Jan Frederik. “Design and fabrication of novel fluorescent nanoparticles for signal generation in immunoassay.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021. http://repository.ust.hk/ir/Record/1783.1-87403 ; https://doi.org/10.14711/thesis-b1626126 ; http://repository.ust.hk/ir/bitstream/1783.1-87403/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Engels, Jan Frederik. “Design and fabrication of novel fluorescent nanoparticles for signal generation in immunoassay.” 2016. Web. 04 Mar 2021.

Vancouver:

Engels JF. Design and fabrication of novel fluorescent nanoparticles for signal generation in immunoassay. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 04]. Available from: http://repository.ust.hk/ir/Record/1783.1-87403 ; https://doi.org/10.14711/thesis-b1626126 ; http://repository.ust.hk/ir/bitstream/1783.1-87403/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Engels JF. Design and fabrication of novel fluorescent nanoparticles for signal generation in immunoassay. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-87403 ; https://doi.org/10.14711/thesis-b1626126 ; http://repository.ust.hk/ir/bitstream/1783.1-87403/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Jones, Debbie Louise. Fluorescence Spectroscopy and Microscopy as Tools for Monitoring Redox Transformations of Uranium in Biological Systems.

Degree: 2017, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306823

► The immobilisation of uranium is an important issue within the nuclear industrydue to contaminated land from accidental spillage, weapons testing or mining activities.Within the environment… (more)

▼ The immobilisation of uranium is an important issue within the nuclear industrydue to contaminated land from accidental spillage, weapons testing or mining activities.Within the environment uranium is most commonly found in the +VI oxidation state asthe mobile uranyl cation [UO2]2+. Alternatively, the +IV oxidation state can also befound in the environment, forming either an insoluble crystalline uraninite phase, or amore soluble molecular uranium(IV) species. Many endogenous subsurface bacteria canbind and accumulate actinide ions through biosorption and can reduce mobileuranyl(VI) species down to immobile uranium(IV) compounds and mineral phases.This work presents an investigation into the bioreduction process by twoanaerobic Gram-negative bacteria, Geobacter sulfurreducens and Shewanellaoneidensis MR-1. Luminescence spectroscopy is used to monitor the intensity ofuranyl(VI) emission in situ over the course of a 24 hour bioreduction experiment withuranyl(VI) acetate as the electron acceptor and either acetate or lactate as the electrondonor. An increase in intensity of the emission around hour three or four during thereduction, followed by an overall decrease, is attributed as the disproportionation of anunstable uranyl(V) intermediate. The role of inner and outer membrane c-typecytochromes as well as flavin secretion is also investigated using three deletion mutantsof the S. oneidensis bacteria, which shows that in their absence, the reduction ofuranyl(VI) does not occur over the course of 24 hours. The emission of uranium(IV) isalso investigated during bioreduction in phosphate media and results show that emissioncan be observed in aqueous solutions at pH 7 pointing to the presence of a molecularproduct.One photon confocal and two photon fluorescence microscopy has been utilisedfor the very first time to directly optically image the bioreduction of uranyl(VI) incombination with luminescence lifetime mapping. The sorption of uranyl(VI) onto thesurface of the bacteria with differing lifetimes indicates a direct interaction betweenuranyl(VI) and surface bound c-type cytochromes, since this variation was not observedin mutant S. oneidensis strains where the cytochromes were not present. Combined,these results have established the applicability of opticalspectroscopy and microscopyin tracking the bioreduction of uranium in situ. Advisors/Committee Members: MORRIS, KATHERINE K, LLOYD, JONATHAN JR, Morris, Katherine, Lloyd, Jonathan, Natrajan, Louise.

Subjects/Keywords: Fluorescence; Luminescence; Spectroscopy; Uranium; Bacteria

10 more images…

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APA (6^th Edition):

Jones, D. L. (2017). Fluorescence Spectroscopy and Microscopy as Tools for Monitoring Redox Transformations of Uranium in Biological Systems. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306823

Chicago Manual of Style (16^th Edition):

Jones, Debbie Louise. “Fluorescence Spectroscopy and Microscopy as Tools for Monitoring Redox Transformations of Uranium in Biological Systems.” 2017. Doctoral Dissertation, University of Manchester. Accessed March 04, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306823.

MLA Handbook (7^th Edition):

Jones, Debbie Louise. “Fluorescence Spectroscopy and Microscopy as Tools for Monitoring Redox Transformations of Uranium in Biological Systems.” 2017. Web. 04 Mar 2021.

Vancouver:

Jones DL. Fluorescence Spectroscopy and Microscopy as Tools for Monitoring Redox Transformations of Uranium in Biological Systems. [Internet] [Doctoral dissertation]. University of Manchester; 2017. [cited 2021 Mar 04]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306823.

Council of Science Editors:

Jones DL. Fluorescence Spectroscopy and Microscopy as Tools for Monitoring Redox Transformations of Uranium in Biological Systems. [Doctoral Dissertation]. University of Manchester; 2017. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:306823

Texas A&M University

10. Bixler, Joel N. Integrating Cavity Enhanced Spectroscopy for Liquid and Gas Sensing.

Degree: PhD, Biomedical Engineering, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/156364

► With continued advances in optical instrumentation coupled with new highly specific contrast methods, optical based spectroscopic techniques continue to find new applications in a wide… (more)

▼ With continued advances in optical instrumentation coupled with new highly specific contrast methods, optical based spectroscopic techniques continue to find new applications in a wide range of fields from medical diagnostics to analytical chemistry. Fluorescence based detection has been used in a variety of medical and environmental sensing applications. Raman spectroscopy has long been used in a variety of industries and applications due to its unique ability to provide label free chemically specific information about a molecule. While both of these techniques have tremendous potential in both medical diagnostics and environmental sensing, often cost efficiency and sensitivity limit more wide spread use. To overcome these limitations, we explore the use of integrating cavity enhanced spectroscopy as a means to greatly enhance the detection sensitivity of both Raman and fluorescence spectroscopy based detection. Using a new diffuse reflector, we have constructed novel integrating cavities that provide significant enhancement of linear optical processes. Enhancement of these processes stems from the long optical pathlength light experiences inside the cavity combined with the relatively large volume that can be probed when compared to traditional spectroscopic approaches. Integrating cavities have several advantages over other sources of signal enhancement in that there is no need for high cost laser sources, and the broad range of wavelengths over which the cavity is reflective affords the ability to use ultraviolet (UV) and blue excitation wavelengths. Raman spectroscopy greatly benefits from the use of more blue shifted sources. Additionally, this allows for one to take advantage of the endogenous fluorescence of many organic molecules that fluoresce under UV excitation. The enhancement of both spontaneous Raman generation as a result of the integrating cavity is demonstrated by measuring the sensitivity of detection for three aromatic hydrocarbons. Fluorescence enhancement is demonstrated by exploring the detection limits of an urobilin zinc phosphor complex, a biomarker that has been shown to be a viable indicator of human and animal waste contamination in water sources. Finally, the design of an integrating cavity system for gas phase analysis will be demonstrated. Advisors/Committee Members: Yakovlev, Vladislav V (advisor), Cote, Gerard L (committee member), Fry, Edward S (committee member), Maitland, Kristen C (committee member).

Subjects/Keywords: Fluorescence spectroscopy; Raman spectroscopy; Biomedical optics

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APA (6^th Edition):

Bixler, J. N. (2015). Integrating Cavity Enhanced Spectroscopy for Liquid and Gas Sensing. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156364

Chicago Manual of Style (16^th Edition):

Bixler, Joel N. “Integrating Cavity Enhanced Spectroscopy for Liquid and Gas Sensing.” 2015. Doctoral Dissertation, Texas A&M University. Accessed March 04, 2021. http://hdl.handle.net/1969.1/156364.

MLA Handbook (7^th Edition):

Bixler, Joel N. “Integrating Cavity Enhanced Spectroscopy for Liquid and Gas Sensing.” 2015. Web. 04 Mar 2021.

Vancouver:

Bixler JN. Integrating Cavity Enhanced Spectroscopy for Liquid and Gas Sensing. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1969.1/156364.

Council of Science Editors:

Bixler JN. Integrating Cavity Enhanced Spectroscopy for Liquid and Gas Sensing. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156364

Georgia State University

11. Goolsby, Demesheka. Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes.

Degree: MS, Chemistry, 2016, Georgia State University

URL: https://scholarworks.gsu.edu/chemistry_theses/91

► Calcium (Ca²⁺), a crucial effector for many biological systems, has been associated with diseases such as cardiovascular disease, Alzheimer’s, Parkinson’s, cancer, and osteoporosis. It… (more)

Subjects/Keywords: Fluorescence; mCherry; EGFP; Calcium imaging; Fluorescence spectroscopy; Fluorescence lifetime

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APA (6^th Edition):

Goolsby, D. (2016). Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/chemistry_theses/91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goolsby, Demesheka. “Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes.” 2016. Thesis, Georgia State University. Accessed March 04, 2021. https://scholarworks.gsu.edu/chemistry_theses/91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goolsby, Demesheka. “Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes.” 2016. Web. 04 Mar 2021.

Vancouver:

Goolsby D. Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes. [Internet] [Thesis]. Georgia State University; 2016. [cited 2021 Mar 04]. Available from: https://scholarworks.gsu.edu/chemistry_theses/91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goolsby D. Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes. [Thesis]. Georgia State University; 2016. Available from: https://scholarworks.gsu.edu/chemistry_theses/91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Κοτσιάς, Δημήτριος. Φασματοσκοπία χρονικής ανάλυσης και διφωτονικής απορρόφησης οργανικών ενώσεων παράγωγων της βενζοδισθιαζόλης.

Degree: 2011, University of Patras

URL: http://hdl.handle.net/10889/5175

►

Στην παρούσα μεταπτυχιακή αυτή εργασία μελετήσαμε την συμπεριφορά για πρώτη φορά ενώσεων που είχαν σαν βάση την βενζοδισθιαζόλη. Συγκεκριμένα οι ενώσεις αυτές μελετήθηκαν με την… (more)

▼

Στην παρούσα μεταπτυχιακή αυτή εργασία μελετήσαμε την συμπεριφορά για πρώτη φορά ενώσεων που είχαν σαν βάση την βενζοδισθιαζόλη. Συγκεκριμένα οι ενώσεις αυτές μελετήθηκαν με την χρήση των τεχνικών της φασματοσκοπίας διφωτονικής απορρόφησης της φασματοσκοπίας σταθερής κατάστασης και της φασματοσκοπίας φθορισμού χρονικής ανάλυσης. Αρχικά όσον αφορά την φασματοσκοπία διφωτονικής απορρόφησης, μπορέσαμε να οδηγηθούμε στα εξής συμπεράσματα: οι καλύτερες ενώσεις που παρουσιάζουν αρκετά μεγάλη διφωτονική απορρόφηση είναι τα γραμμικά μόρια (PK-439 και PK-452) σε σχέση με τα U-shaped μόρια με μέγιστη ενεργό διατομή ΔΦΑ ~2000GM. Επιπλέον παρατηρήσαμε ότι η χρήση της βενζοδισθιαζόλης σαν κεντρικός πυρήνας προκαλεί σημαντική αύξηση της διφωτονικής απορρόφησης, σε σχέση με την βενζοθιαζόλη. Τέλος, με την τεχνική της φασματοσκοπίας φθορισμού χρονικής ανάλυσης μπορέσαμε να οδηγηθούμε σε κάποιες διαπιστώσεις: συγκεκριμένα παρατηρήσαμε ότι όσο το μήκος κύματος καταγραφής μεγαλώνει, τόσο οι καμπύλες αποδιέγερσης γίνονται πιο αργές. Ακόμα διαπιστώσαμε ότι από την σύγκριση μορίων στο μήκος κύματος του μεγίστου, σε εκείνα τα μόρια που αποδιεγείρονται γρήγορα, ευνοούνται οι μη-ακτινοβολητικές διεργασίες και ταυτόχρονα παρουσιάζουν μικρή ενεργό διατομή ΔΦΑ.

–

Advisors/Committee Members: Περσεφόνης, Πέτρος, Kotsias, Dimitrios, Περσεφόνης, Πέτρος, Φακής, Μιχαήλ, Γιαννέτας, Βασίλειος.

Subjects/Keywords: Φθορισμός; Διφωτονική απορρόφηση; 543.56; Fluorescence; Time-resolved fluorescence spectroscopy; Steady state fluorescence spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Κοτσιάς, �. (2011). Φασματοσκοπία χρονικής ανάλυσης και διφωτονικής απορρόφησης οργανικών ενώσεων παράγωγων της βενζοδισθιαζόλης. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/5175

Chicago Manual of Style (16^th Edition):

Κοτσιάς, Δημήτριος. “Φασματοσκοπία χρονικής ανάλυσης και διφωτονικής απορρόφησης οργανικών ενώσεων παράγωγων της βενζοδισθιαζόλης.” 2011. Masters Thesis, University of Patras. Accessed March 04, 2021. http://hdl.handle.net/10889/5175.

MLA Handbook (7^th Edition):

Κοτσιάς, Δημήτριος. “Φασματοσκοπία χρονικής ανάλυσης και διφωτονικής απορρόφησης οργανικών ενώσεων παράγωγων της βενζοδισθιαζόλης.” 2011. Web. 04 Mar 2021.

Vancouver:

Κοτσιάς �. Φασματοσκοπία χρονικής ανάλυσης και διφωτονικής απορρόφησης οργανικών ενώσεων παράγωγων της βενζοδισθιαζόλης. [Internet] [Masters thesis]. University of Patras; 2011. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10889/5175.

Council of Science Editors:

Κοτσιάς �. Φασματοσκοπία χρονικής ανάλυσης και διφωτονικής απορρόφησης οργανικών ενώσεων παράγωγων της βενζοδισθιαζόλης. [Masters Thesis]. University of Patras; 2011. Available from: http://hdl.handle.net/10889/5175

University of Texas – Austin

13. Anderson, Cari Michelle. Investigating molecular effects on membrane structure, dynamics and function.

Degree: PhD, Chemistry, 2019, University of Texas – Austin

URL: http://dx.doi.org/10.26153/tsw/2180

► Biological membranes are heterogeneous structures with complex electrostatic profiles arising from lipids, sterols, membrane proteins, and water molecules. We investigated the effect of cholesterol and… (more)

▼ Biological membranes are heterogeneous structures with complex electrostatic profiles arising from lipids, sterols, membrane proteins, and water molecules. We investigated the effect of cholesterol and its derivative 6-ketocholestanol (6-kc) on membrane electrostatics by directly measuring the dipole electric field (F [arrow above F] [subscript d] ) within lipid bilayers containing cholesterol or 6-kc at concentrations of 0−40 mol% through the vibrational Stark effect (VSE). We found that adding low concentrations of cholesterol, up to ∼10 mol %, increases F [arrow above F] [subscript d], while adding more cholesterol up to 40 mol% lowers F [arrow above F] [subscript d]. In contrast, we measured a monotonic increase in F [arrow above F] [subscript d] as 6-kc concentration increased. We proposed that this membrane electric field is affected by multiple factors: the polarity of the sterol molecules, the reorientation of the phospholipid dipole due to sterol, and the impact of the sterol on hydrogen bonding with surface water. We used molecular dynamics simulations to examine the distribution of phospholipids, sterol, and helix in bilayers containing these sterols. At low concentrations, we observed clustering of sterols near the vibrational probe whereas at high concentrations, we observed spatial correlation between the positions of the sterol molecules. This work demonstrated how a one-atom difference in a sterol changes the physicochemical and electric field properties of the bilayer. Additionally, we set out to understand how a small molecule interacts with the lipid bilayer differently based on its charge. Our laboratory had previously reported that tryptophan permeated through a phosphatidylcholine lipid bilayer membrane at a faster rate when it was positively charged (Trp+) than when negatively charged (Trp−), which corresponded to a lower potential of mean force (PMF) barrier determined through simulations. In the work described here, we demonstrated that Trp+ partitions into the lipid bilayer membrane to a greater degree than Trp− by interacting with the ester linkage of a phosphatidylcholine lipid, where it is stabilized by the electron withdrawing glycerol functional group. These results are in agreement with tryptophan’s known role as an anchor for transmembrane proteins, though the tendency for binding of a positively charged tryptophan is surprising. We discussed the implications of our results on the mechanisms of unassisted permeation and penetration of small molecules within and across lipid bilayer membranes based on molecular charge, shape, and molecular interactions within the bilayer structure. Advisors/Committee Members: Webb, Lauren J. (advisor), Elber, Ron (committee member), Baiz, Carlos R (committee member), Eberlin, Livia S (committee member), Gordon, Vernita (committee member).

Subjects/Keywords: Membranes; Vesicles; Biophysics; Spectroscopy; Fluorescence spectroscopy; Vibrational spectroscopy; Cholesterol; 6-ketocholestanol; Tryptophan; Fluorescence quenching

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Anderson, C. M. (2019). Investigating molecular effects on membrane structure, dynamics and function. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://dx.doi.org/10.26153/tsw/2180

Chicago Manual of Style (16^th Edition):

Anderson, Cari Michelle. “Investigating molecular effects on membrane structure, dynamics and function.” 2019. Doctoral Dissertation, University of Texas – Austin. Accessed March 04, 2021. http://dx.doi.org/10.26153/tsw/2180.

MLA Handbook (7^th Edition):

Anderson, Cari Michelle. “Investigating molecular effects on membrane structure, dynamics and function.” 2019. Web. 04 Mar 2021.

Vancouver:

Anderson CM. Investigating molecular effects on membrane structure, dynamics and function. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2019. [cited 2021 Mar 04]. Available from: http://dx.doi.org/10.26153/tsw/2180.

Council of Science Editors:

Anderson CM. Investigating molecular effects on membrane structure, dynamics and function. [Doctoral Dissertation]. University of Texas – Austin; 2019. Available from: http://dx.doi.org/10.26153/tsw/2180

Vanderbilt University

14. Yirdaw, Robel Birru. Conformational dynamics of proteins by fluorescence fluctuation spectroscopy.

Degree: PhD, Physics, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/12826

► Proteins, as intrinsically flexible molecules, exhibit internal motions at equilibrium. In general, the internal motions consist of changes in the three dimensional coordinates of the… (more)

▼ Proteins, as intrinsically flexible molecules, exhibit internal motions at equilibrium. In general, the internal motions consist of changes in the three dimensional coordinates of the constituent atoms. These motions are collectively referred to as conformational dynamics and span multiple orders of magnitude in timescale. Protein conformational dynamics is central to biological processes. In this work, fluorescence fluctuation spectroscopy (FFS), a single molecule technique, was utilized to study the conformational dynamics of Bacteriophage T4 lysozyme (T4L). Bacteriophage T4 is a virus that infects <i>E. coli</i> and T4L is used to break down the <i>E. coli</i> cell wall in the late stages of the infection cycle. The structure of T4L consists of two domains joined by a long helix with the active site situated in between the two domains. The presence of equilibrium conformational dynamics in T4L consisting of the motion of one domain relative to the other has long been postulated. In the work presented here, fluorescence correlation spectroscopy (FCS) and brightness (cumulant) analysis, two complementary variations of FFS, were used to investigate T4 lysozyme conformational dynamics. FCS results give direct evidence for conformational dynamics in T4L consisting of changes in relative distance and orientation of the two domains with a relaxation time of approximately 15 µs. The amplitude of this motion diminishes upon covalent substrate trapping. FCS results indicate the presence of dynamics along the long helix that persists upon substrate binding which may be necessary to facilitate the opening and closing of the active site. Furthermore, in contrast to a motion that involves discrete open and closed conformational states, results point to the presence of multiple conformations. Moreover, FFS was applied on Ca²⁺/calmodulin-dependent protein kinase II (CAMKII) and the membrane transporter MsbA. Relative to T4L, these two serve as examples of complex protein systems. The particular challenges in applying FFS to such systems are presented. The use of brightness analysis, a relatively new approach compared to FCS, for the study of protein conformational dynamics is discussed. As part of this work, data analysis and simulation software tools were developed using MATLAB. Advisors/Committee Members: David W. Piston (committee member), Thomas J. Weiler (committee member), Kalman Varga (committee member), M. Shane Hutson (committee member), Hassane S. Mchaourab (Committee Chair).

Subjects/Keywords: Single Molecule Fluorescence Spectroscopy; T4 Lysozyme; Fluorescence Fluctuation Spectroscopy; Conformational Dynamics; Protein; Fluorescence Correlation Spectroscopy; Brightness Analysis; Cumulant Analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yirdaw, R. B. (2012). Conformational dynamics of proteins by fluorescence fluctuation spectroscopy. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12826

Chicago Manual of Style (16^th Edition):

Yirdaw, Robel Birru. “Conformational dynamics of proteins by fluorescence fluctuation spectroscopy.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 04, 2021. http://hdl.handle.net/1803/12826.

MLA Handbook (7^th Edition):

Yirdaw, Robel Birru. “Conformational dynamics of proteins by fluorescence fluctuation spectroscopy.” 2012. Web. 04 Mar 2021.

Vancouver:

Yirdaw RB. Conformational dynamics of proteins by fluorescence fluctuation spectroscopy. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1803/12826.

Council of Science Editors:

Yirdaw RB. Conformational dynamics of proteins by fluorescence fluctuation spectroscopy. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/12826

NSYSU

15. Wang, Yen-sheng. The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution.

Degree: Master, Electro-Optical Engineering, 2008, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905

► Luminescent conjugated polymers are widely used in organic optoelectronics. The device is fabricated by spin coating the polymer solutions into thin films. It is important… (more)

Subjects/Keywords: MDMO-PPV; Fluorescence correlation spectroscopy (FCS)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Y. (2008). The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Yen-sheng. “The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution.” 2008. Thesis, NSYSU. Accessed March 04, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Yen-sheng. “The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution.” 2008. Web. 04 Mar 2021.

Vancouver:

Wang Y. The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution. [Internet] [Thesis]. NSYSU; 2008. [cited 2021 Mar 04]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang Y. The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rochester Institute of Technology

16. Sood, Harshita. Investigating Annual Variation in Fruit Quality Using Nutrient Assays and Multidimensional Fluorescence Spectroscopy.

Degree: MS, Thomas H. Gosnell School of Life Sciences (COS), 2015, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/8803

► Each year, billions of birds engage in migratory behavior in response to seasonal changes. During fall migration, many birds consume nutritionally rich fruits with… (more)

Subjects/Keywords: Avian nutrition; Climate change; Fluorescence spectroscopy; PARAFAC

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sood, H. (2015). Investigating Annual Variation in Fruit Quality Using Nutrient Assays and Multidimensional Fluorescence Spectroscopy. (Masters Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/8803

Chicago Manual of Style (16^th Edition):

Sood, Harshita. “Investigating Annual Variation in Fruit Quality Using Nutrient Assays and Multidimensional Fluorescence Spectroscopy.” 2015. Masters Thesis, Rochester Institute of Technology. Accessed March 04, 2021. https://scholarworks.rit.edu/theses/8803.

MLA Handbook (7^th Edition):

Sood, Harshita. “Investigating Annual Variation in Fruit Quality Using Nutrient Assays and Multidimensional Fluorescence Spectroscopy.” 2015. Web. 04 Mar 2021.

Vancouver:

Sood H. Investigating Annual Variation in Fruit Quality Using Nutrient Assays and Multidimensional Fluorescence Spectroscopy. [Internet] [Masters thesis]. Rochester Institute of Technology; 2015. [cited 2021 Mar 04]. Available from: https://scholarworks.rit.edu/theses/8803.

Council of Science Editors:

Sood H. Investigating Annual Variation in Fruit Quality Using Nutrient Assays and Multidimensional Fluorescence Spectroscopy. [Masters Thesis]. Rochester Institute of Technology; 2015. Available from: https://scholarworks.rit.edu/theses/8803

University of Utah

17. Myers, Grant Aaron. Characterizing the interactions of molecules with phospholipid vesicles and bilayers by confocal raman and single-molecule fluorescence microscopy.

Degree: PhD, Chemistry, 2012, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/1922/rec/447

► Molecular interactions with phospholipid bilayers are investigated using two types of laser-based microscopies. In optical-trapping confocal Raman microscopy, individual phospholipid vesicles are held in place… (more)

▼ Molecular interactions with phospholipid bilayers are investigated using two types of laser-based microscopies. In optical-trapping confocal Raman microscopy, individual phospholipid vesicles are held in place in situ with laser tweezers while Raman scattering spectra are collected to report the preconcentration of analyte molecules via pH-gradient loading. A theory of accumulation is developed to account for source-depletion and vesicle buffering, factors that can significantly reduce the total accumulation. The system of 10 equations including 10 unknowns, five equilibrium constants and five experimental parameters was solved with symbolic-based mathematics software to yield a sixth-order polynomial having a single physically-meaningful, positive, real root. Utilizing this theory, an experiment is designed in which a single vesicle is manipulated by optical tweezers into isolation from other interfering vesicles and subjected to a variety of solution conditions. Raman spectra are analyzed quantitatively with a classical least squares method to determine the concentrations of compounds inside of the vesicle. When the pH gradient is 6.5 units and the citric-acid buffer inside of vesicles is 0.5 M, a 104-fold preconcentration factor of a model compound benzyl-dimethyl amine is observed, where accumulation into a 600-nm diameter vesicle is complete in less than 20 min.Total-internal-reflection fluorescence (TIRF) microscopy is a technique with the dynamic range, spatial and temporal resolution capable of characterizing the interactionsivof single fluorescently labeled peptides with a planar supported lipid bilayer. TIRF images are analyzed with an adjacent pixel criterion to discriminate between molecular events and random noise exceeding the threshold. The bi-exponential distribution of event durations is interpreted with respect to a consecutive reversible three-state kinetics model, where unfolded peptides from solution first interact weakly with the bilayer then fold to persist for a longer time. Application of this model is shown to be capable of transforming the measured event times into the microscopic rates of the system including the rates of peptide folding and unfolding. The Gibb’s free energy of the folding reaction from these measured rates, 1.7 kJ⋅mol-1 is comparable to that of other helix-forming membrane active peptides measured by isothermal titration calorimetry.

Subjects/Keywords: Fluorescence; Microscopy; Raman; Single-molecule; Spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Myers, G. A. (2012). Characterizing the interactions of molecules with phospholipid vesicles and bilayers by confocal raman and single-molecule fluorescence microscopy. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/1922/rec/447

Chicago Manual of Style (16^th Edition):

Myers, Grant Aaron. “Characterizing the interactions of molecules with phospholipid vesicles and bilayers by confocal raman and single-molecule fluorescence microscopy.” 2012. Doctoral Dissertation, University of Utah. Accessed March 04, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/1922/rec/447.

MLA Handbook (7^th Edition):

Myers, Grant Aaron. “Characterizing the interactions of molecules with phospholipid vesicles and bilayers by confocal raman and single-molecule fluorescence microscopy.” 2012. Web. 04 Mar 2021.

Vancouver:

Myers GA. Characterizing the interactions of molecules with phospholipid vesicles and bilayers by confocal raman and single-molecule fluorescence microscopy. [Internet] [Doctoral dissertation]. University of Utah; 2012. [cited 2021 Mar 04]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/1922/rec/447.

Council of Science Editors:

Myers GA. Characterizing the interactions of molecules with phospholipid vesicles and bilayers by confocal raman and single-molecule fluorescence microscopy. [Doctoral Dissertation]. University of Utah; 2012. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/1922/rec/447

Oregon State University

18. Neyzari, Shahrokh Ghaffari. Design and application of a microcomputer automated multielement flame atomic fluorescence instrument.

Degree: PhD, Chemistry, 1984, Oregon State University

URL: http://hdl.handle.net/1957/40704

► An automated multielement flame atomic fluorescence (AF) spectrometer based on microcomputer control was constructed to determine four elements simultaneously. The instrument employs a multiple exit… (more)

▼ An automated multielement flame atomic fluorescence (AF) spectrometer based on microcomputer control was constructed to determine four elements simultaneously. The instrument employs a multiple exit slit monochromator where the light from various exit slits is directed to a single detector with a mirrored funnel. Each element is excited to fluoresce with a single element hollow cathode lamp (HCL) and a time multiplex mode is used for pulsing the HCL's and data acquisition. A SYM-1 microcomputer is the center of the system and controls the pulsing of HCL's, sample introduction into the flame, data acquisition, and other electronic components. Values for the experimental variables such as the HCL pulse rate, peak current, and pulse width are selected at the computer's terminal. The HCL and background signals for each element are integrated for equal periods of time selected from 0.5 to 250 ms. The integrated background (lamp off) signal is subtracted from the HCL (lamp on) signal to correct for the non-lamp related portion of the signal. For most measurements, an air/H₂ flame sheathed with Ar was the atomizer. Numerous experimental variables were optimized including HCL current and fuel and oxidant flow rates. For single element flame AF under optimum conditions, the following detection limits (in ng/mL) were obtained: Au, 2.2 x 10²; Cd, 4.8; Co, 26; Cu, 3; Fe, 50; Mg, 0.8; Mn, 7; Ni, 36; Pb, 1.0 x 10³ ; Zn, 21. For multielement flame AF under compromise conditions, the following detection limits (in ng/mL) were obtained: Cd, 10; Cu, 4; Mg, 1.5 x 10²; Zn, 1.1 x 10². With a sheathed air/C₂H₂ flame in the multielement mode, the following detection limits (in ng/mL) were obtained: Cd, 7.7 x 10²; Cu, 1.3 x 10²; Mg, 18; Zn, 4.3 x 10³. The higher flame background emission noise degraded detection limits compared to the air/H₂ flame. The system was studied briefly in a nondispersive multielement AF mode with a set of filters installed directly in front of the detector window. The following detection limits (in ng/mL) were obtained under multielement flame AF conditions with an air/H₂ flame: Cu, 33; Mg, 16; Mn, 76. The relative standard deviation of measurements for single and multiple element determinations was typically 1% or better for concentrations well above the detection limit. Advisors/Committee Members: Ingle, James D. Jr. (advisor), Krueger, James H. (committee member).

Subjects/Keywords: Fluorescence spectroscopy – Automation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Neyzari, S. G. (1984). Design and application of a microcomputer automated multielement flame atomic fluorescence instrument. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/40704

Chicago Manual of Style (16^th Edition):

Neyzari, Shahrokh Ghaffari. “Design and application of a microcomputer automated multielement flame atomic fluorescence instrument.” 1984. Doctoral Dissertation, Oregon State University. Accessed March 04, 2021. http://hdl.handle.net/1957/40704.

MLA Handbook (7^th Edition):

Neyzari, Shahrokh Ghaffari. “Design and application of a microcomputer automated multielement flame atomic fluorescence instrument.” 1984. Web. 04 Mar 2021.

Vancouver:

Neyzari SG. Design and application of a microcomputer automated multielement flame atomic fluorescence instrument. [Internet] [Doctoral dissertation]. Oregon State University; 1984. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1957/40704.

Council of Science Editors:

Neyzari SG. Design and application of a microcomputer automated multielement flame atomic fluorescence instrument. [Doctoral Dissertation]. Oregon State University; 1984. Available from: http://hdl.handle.net/1957/40704

Cornell University

19. Van Slyke, Alexander LeRoy. DEVELOPMENT OF NOVEL METHODS FOR IMAGING MEMBRANE PROTEIN STOICHIOMETRY AT THE SINGLE MOLECULE LEVEL AND FOR FABRICATION OF A SINGLE MOLECULE PROTEIN-DNA IMAGING DEVICE.

Degree: PhD, Biophysics, 2019, Cornell University

URL: http://hdl.handle.net/1813/67625

► Single molecule fluorescence imaging techniques have revolutionized the way we study biology by offering methods to examine the behavior and arrangement of individual molecules and… (more)

▼ Single molecule fluorescence imaging techniques have revolutionized the way we study biology by offering methods to examine the behavior and arrangement of individual molecules and the molecular mechanisms underlying biological processes. These techniques permit the investigation of transient states and critical heterogeneities undetectable by ensemble measurements and genome-wide assays. In this dissertation, I detail a series of projects, performed as a member of the laboratory of Warren Zipfel, in which new single molecule methods were created and applied to address important biological questions. A technique designed to study protein-DNA interactions at the single molecule level in a high-throughput fashion is called “DNA curtains.” The nanopatterned microfluidic devices necessary for these experiments have previously been fabricated using electron-beam lithography. We developed a simplified, cost effective, and more accessible method of fabricating these devices. Due to their modular DNA binding domain, transcription activator-like effectors (TALEs) have potential to be used in the study of gene function and gene editing with medical and agricultural applications. Understanding the target search mechanism of TALEs is important to developing more efficient and accurate ways to design and deliver TALE proteins. In my first project, we investigated TALEs using “DNA curtains” in an effort to elucidate the details of this search mechanism. Many single molecule techniques require the sample to be observed in vitro in order to isolate the biomolecule of interest. As a result, physiological behavior may not be preserved. In my second project, we developed a method named Single Protein Recovery After Dilution (SPReAD) that addresses this limitation by enabling protein stoichiometry and function to be studied in vivo. My final project is an investigation of the functional composition of metabotropic glutamate receptors (mGluRs). Glutamate acts as both a neurotransmitter and neuromodulator in the central nervous system. These neuromodulatory effects are mediated by mGluRs and their improper function has been linked to schizophrenia and Fragile X Syndrome. Understanding the stoichiometry of mGluR complexes is necessary to the development of pharmacological compounds which modulate their signaling. We investigated the interaction between Group I mGluRs at the single molecule level in vivo utilizing our SPReAD technique. Advisors/Committee Members: Zipfel, Warren R. (chair), Pollack, Lois (committee member), Lis, John T. (committee member).

Subjects/Keywords: Single Molecule; Microscopy; Fluorescence; Optical Spectroscopy; Biophysics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Slyke, A. L. (2019). DEVELOPMENT OF NOVEL METHODS FOR IMAGING MEMBRANE PROTEIN STOICHIOMETRY AT THE SINGLE MOLECULE LEVEL AND FOR FABRICATION OF A SINGLE MOLECULE PROTEIN-DNA IMAGING DEVICE. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/67625

Chicago Manual of Style (16^th Edition):

Van Slyke, Alexander LeRoy. “DEVELOPMENT OF NOVEL METHODS FOR IMAGING MEMBRANE PROTEIN STOICHIOMETRY AT THE SINGLE MOLECULE LEVEL AND FOR FABRICATION OF A SINGLE MOLECULE PROTEIN-DNA IMAGING DEVICE.” 2019. Doctoral Dissertation, Cornell University. Accessed March 04, 2021. http://hdl.handle.net/1813/67625.

MLA Handbook (7^th Edition):

Van Slyke, Alexander LeRoy. “DEVELOPMENT OF NOVEL METHODS FOR IMAGING MEMBRANE PROTEIN STOICHIOMETRY AT THE SINGLE MOLECULE LEVEL AND FOR FABRICATION OF A SINGLE MOLECULE PROTEIN-DNA IMAGING DEVICE.” 2019. Web. 04 Mar 2021.

Vancouver:

Van Slyke AL. DEVELOPMENT OF NOVEL METHODS FOR IMAGING MEMBRANE PROTEIN STOICHIOMETRY AT THE SINGLE MOLECULE LEVEL AND FOR FABRICATION OF A SINGLE MOLECULE PROTEIN-DNA IMAGING DEVICE. [Internet] [Doctoral dissertation]. Cornell University; 2019. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1813/67625.

Council of Science Editors:

Van Slyke AL. DEVELOPMENT OF NOVEL METHODS FOR IMAGING MEMBRANE PROTEIN STOICHIOMETRY AT THE SINGLE MOLECULE LEVEL AND FOR FABRICATION OF A SINGLE MOLECULE PROTEIN-DNA IMAGING DEVICE. [Doctoral Dissertation]. Cornell University; 2019. Available from: http://hdl.handle.net/1813/67625

Texas A&M University

20. Bixler, Joel Nathan. Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging.

Degree: MS, Biomedical Engineering, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153844

► We have integrated a fluorescence microendoscope into a whole-animal optical imaging system, allowing for simultaneous microscopic and macroscopic imaging of tdTomato expressing BCG in vivo.… (more)

Subjects/Keywords: Fluorescence spectroscopy; Bacterial Imaging; Tuberculosis; microendoscope

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APA (6^th Edition):

Bixler, J. N. (2014). Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153844

Chicago Manual of Style (16^th Edition):

Bixler, Joel Nathan. “Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging.” 2014. Masters Thesis, Texas A&M University. Accessed March 04, 2021. http://hdl.handle.net/1969.1/153844.

MLA Handbook (7^th Edition):

Bixler, Joel Nathan. “Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging.” 2014. Web. 04 Mar 2021.

Vancouver:

Bixler JN. Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1969.1/153844.

Council of Science Editors:

Bixler JN. Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153844

Penn State University

21. Li, Xiang. Ultrafast Charge Transfer �in Conventional Solvent �and Ionic Liquids .

Degree: 2011, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/11674

► This thesis work is aimed at understanding ultrafast intermolecular electron transfer and solvation processes in conventional solvents and ionic liquids. Following a brief introduction in… (more)

▼ This thesis work is aimed at understanding ultrafast intermolecular electron transfer and solvation processes in conventional solvents and ionic liquids. Following a brief introduction in Chapter 1, Chapter 2 explains the main experimental techniques and data analysis methods used in this thesis. Chapter 3 and Chapter 4 are focused on charge transfer reactions in representative ionic liquids in comparison to conventional solvents. Chapter 5 carefully examines a selected system for studying dynamic heterogeneity studies in ionic liquids. Charge transfer is an important step in many types of chemical and biochemical reactions. The energetics and rates of charge transfer are greatly affected by the polarity and dynamics of the surrounding medium and extensive research has explored the effect of polar solvents on charge transfer. In contrast, despite the large amount of attention on ionic liquids for many applications, few studies have focused on fundamental aspects of charge transfer ionic liquids. I have surveyed the behavior of several solvent-controlled intramolecular charge transfer reactions in representative ionic liquids and compared it to what is found for these same reactions in conventional dipolar solvents using time-resolved fluorescence spectroscopy. In chapter 3, crystal violet lactone (CVL), which exhibits distinct dual emission in fluid solution as a result of a rapid excited-state charge transfer reaction, was measured in series of conventional solvents as background for ionic liquids studies. Solvatochromic analysis using a dielectric continuum model suggests dipole moments of 9-12 D for the initially excited (LE) state and ~24 D for the charge-transfer (CT) state. Intensities of steady-state emission as well as kinetic data provide free energies for the LE → CT reaction that range from +12 kJ/mol in nonpolar solvents to -10 kJ/mol in highly polar solvents at 25 C. Reaction rates constants, which lie in the range of 10-100 ns-1 in most solvents, depend on both solvent polarity and solvent friction. In highly polar solvents, this charge transfer reaction was confirmed to follow two-state kinetics as used in typical dual fluorescence probes like aminobenzonitriles. Reaction rates are correlated to solvation times in a manner that indicates the reaction is a solvent-controlled electron transfer on an adiabatic potential surface having a modest barrier. In Chapter 4, the charge transfer reaction of CVL was studied in ionic liquids. Compared with conventional solvents, the reaction is much more complicated. Equilibrium is not reached in any ionic liquid due to the much slower reaction. Furthermore, in two other well-studied charge transfer probes in several classes of ionic liquids, we also found the reaction kinetics are typically more complicated. Multi-exponential decays are observed when reaction times are comparable to solvation times, which are on the nanosecond scale in most ionic liquids at room temperature. Time-resolved spectra were dissected into LE and CT states… Advisors/Committee Members: Mark Maroncelli, Dissertation Advisor/Co-Advisor, Mark Maroncelli, Committee Chair/Co-Chair, Tom Mallouk, Committee Member, John B Asbury, Committee Member, John H Golbeck, Committee Member.

Subjects/Keywords: Ultrafast Fluorescence Spectroscopy; Ionic Liquids; Charge Transfer

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APA (6^th Edition):

Li, X. (2011). Ultrafast Charge Transfer �in Conventional Solvent �and Ionic Liquids . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/11674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Xiang. “Ultrafast Charge Transfer �in Conventional Solvent �and Ionic Liquids .” 2011. Thesis, Penn State University. Accessed March 04, 2021. https://submit-etda.libraries.psu.edu/catalog/11674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Xiang. “Ultrafast Charge Transfer �in Conventional Solvent �and Ionic Liquids .” 2011. Web. 04 Mar 2021.

Vancouver:

Li X. Ultrafast Charge Transfer �in Conventional Solvent �and Ionic Liquids . [Internet] [Thesis]. Penn State University; 2011. [cited 2021 Mar 04]. Available from: https://submit-etda.libraries.psu.edu/catalog/11674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li X. Ultrafast Charge Transfer �in Conventional Solvent �and Ionic Liquids . [Thesis]. Penn State University; 2011. Available from: https://submit-etda.libraries.psu.edu/catalog/11674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

22. Omrani, Hengameh. Health Monitoring of Machinery Fluids Using Excitation-Emission Matrix Spectroscopy and Cavity Ring-Down Spectroscopy .

Degree: Chemistry, 2014, Queens University

URL: http://hdl.handle.net/1974/12082

► The quality of machinery liquids plays a critical role in ensuring safe and cost-effective operation of engines. Especially in the aviation industry, there is a… (more)

▼ The quality of machinery liquids plays a critical role in ensuring safe and cost-effective operation of engines. Especially in the aviation industry, there is a great need for real-time and online monitoring of the purity, lubricity and age of machinery fluids. In this work, two optical techniques, excitation-emission matrix spectroscopy (EEMS) and cavity ring-down spectroscopy (CRDS), are used for monitoring of degradation and contamination of aero-turbine lubricants and jet fuels using optical fiber probes. We implement EEMS combined with a modified fiber probe design to characterize lubricant quality through the characteristic fluorescence of antioxidant additives. Multi-way analysis procedures, such as parallel factor analysis, are applied to correlate spectral features to antioxidant concentration, oxidative stability, and lubricant age. The spectroscopic data are then correlated to commonly used, off-line parameters such as the induction time and the breakdown number. It is shown that the decrease in fluorescence intensities of antioxidants coincides with the decomposition of the oil base stock. The induction times of synthetic jet turbine oil degraded at 150ºC, 195ºC and 215ºC are found to be at about 10,000, 3,500 and 400 min respectively. Simple kinetic models are developed that are capable of describing antioxidant reactions as pseudo first-order processes. We also demonstrate that with fluorescence detection it is possible to determine the concentration of oil contamination in jet fuel from about 10 to 1000 ppmv. In addition, a fiber-loop cavity ring-down spectrometer has been developed to quantitatively identify oil contamination of jet fuel by measuring optical absorption in the UV region. CRDS is a very sensitive, path-enhanced absorption technique that may be used for trace-species measurements in gas and liquids. The absorption measurements on samples with small volumes are characterized by measuring the concentration of turbine oil in jet fuel from 100 000 ppmv to a limit of detection of 400 ppmv. In summary, the obtained results permit us to specify the life time of lubrication oil and to determine the contamination of jet fuel with turbine oil qualitatively and quantitatively. In a simple optical configuration the fiber-coupled EEM and CRD methods permit in situ sampling of the machinery fluids.

Subjects/Keywords: Fluorescence Spectroscopy ; Fiber-Optics ; Chemometrics ; Lubricant Oil

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APA (6^th Edition):

Omrani, H. (2014). Health Monitoring of Machinery Fluids Using Excitation-Emission Matrix Spectroscopy and Cavity Ring-Down Spectroscopy . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/12082

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Omrani, Hengameh. “Health Monitoring of Machinery Fluids Using Excitation-Emission Matrix Spectroscopy and Cavity Ring-Down Spectroscopy .” 2014. Thesis, Queens University. Accessed March 04, 2021. http://hdl.handle.net/1974/12082.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Omrani, Hengameh. “Health Monitoring of Machinery Fluids Using Excitation-Emission Matrix Spectroscopy and Cavity Ring-Down Spectroscopy .” 2014. Web. 04 Mar 2021.

Vancouver:

Omrani H. Health Monitoring of Machinery Fluids Using Excitation-Emission Matrix Spectroscopy and Cavity Ring-Down Spectroscopy . [Internet] [Thesis]. Queens University; 2014. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1974/12082.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Omrani H. Health Monitoring of Machinery Fluids Using Excitation-Emission Matrix Spectroscopy and Cavity Ring-Down Spectroscopy . [Thesis]. Queens University; 2014. Available from: http://hdl.handle.net/1974/12082

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Connecticut

23. Longo, Kaitlyn M. Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis.

Degree: MS, Biomedical Engineering, 2014, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/594

► Otitis Media is defined as inflammation of the middle ear region associated with middle ear fluid. This condition typically follows an upper respiratory infection,… (more)

▼ Otitis Media is defined as inflammation of the middle ear region associated with middle ear fluid. This condition typically follows an upper respiratory infection, is commonly misdiagnosed by physicians and can potentially lead to the inappropriate use of antimicrobial agents. ^[1] This thesis describes the design of a novel medical device that utilized spectroscopy and digital imaging to provide physicians with a unique representation of the middle ear. Published literature has been reviewed to assess the different approaches that researchers have studied to improve the diagnosis of otitis media, where, for example, research conducted by Thorton et al. and Boppart et al. used fluorescence to identify bacteria contained in biofilms.^[12,13] In addition, a population study conducted by Jensen et al. determined what the level of diagnostic certainty is in pediatric patients and determined that general practitioners had a diagnostic certainty of 58% in patients under one year of age and 73% certainty in patients over two and a half years of age.^[9] Eleven subjects, each with a history of recurrent acute otitis media and requiring myringotomy and ear tube placement, were selected for study in this thesis. All subjects received a digital imaging and spectroscopy examination using the device at the time of surgery. Five excitation and illumination wavelengths were used, including white light, 385-420 nm, 450-470 nm, 520-535 nm, and 640-650 nm as well as fluorescence imaging using 425 nm and 450 nm filters. Each of the chosen wavelengths were emitted into the ear canal and tympanic membrane during a typical otoscopic examination. Signal processing techniques were also applied to isolate various regions of the tympanic membrane using MatLAB to increase the resolution of the anatomical and physiological properties of the tympanic membrane. The spectral readings between the wavelengths of 560 nm and 760 nm were plotted for each subject and were classified according to fluid retention, consistency, and vascularity. In this subject population, eight of the 22 ears were identified to be a noneffusion normal. Reflectance of keratin, cerumen, and bone, with well defined blood vessels and contrast, allowed for better characterization of the middle ear. ^[8] Reflectance spectra were classified according to fluid consistency and trends were determined for non effusion and effusion patients. Class one spectra were defined as tympanic membrane and canal spectra with a rapid increase in percent reflection between 575 nm and 650 nm and a rapid decrease in percent reflection between 650 nm and 750 nm. Class two spectra were defined as tympanic membrane and canal spectra with a gradual decrease in percent reflection between 575 nm and 800 nm. This novel device was successfully used to detect effusion consistency and color and it will continue to aid in the development of a new diagnostic procedure. It provides a means for the creation of a new diagnostic procedure that may be useful… Advisors/Committee Members: Tulio Valdez, Martin Cherniack, Donald Peterson.

Subjects/Keywords: Otitis Media; Otolaryngology; Digital Imaging; Fluorescence; Spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Longo, K. M. (2014). Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/594

Chicago Manual of Style (16^th Edition):

Longo, Kaitlyn M. “Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis.” 2014. Masters Thesis, University of Connecticut. Accessed March 04, 2021. https://opencommons.uconn.edu/gs_theses/594.

MLA Handbook (7^th Edition):

Longo, Kaitlyn M. “Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis.” 2014. Web. 04 Mar 2021.

Vancouver:

Longo KM. Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis. [Internet] [Masters thesis]. University of Connecticut; 2014. [cited 2021 Mar 04]. Available from: https://opencommons.uconn.edu/gs_theses/594.

Council of Science Editors:

Longo KM. Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis. [Masters Thesis]. University of Connecticut; 2014. Available from: https://opencommons.uconn.edu/gs_theses/594

East Carolina University

24. Angermeier, Tori Marena. Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers.

Degree: MS, MS-Chemistry, 2018, East Carolina University

URL: http://hdl.handle.net/10342/6929

► It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between… (more)

Subjects/Keywords: lipid; Sugar – Analysis; Disaccharides; Fluorescence spectroscopy

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APA (6^th Edition):

Angermeier, T. M. (2018). Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers. (Masters Thesis). East Carolina University. Retrieved from http://hdl.handle.net/10342/6929

Chicago Manual of Style (16^th Edition):

Angermeier, Tori Marena. “Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers.” 2018. Masters Thesis, East Carolina University. Accessed March 04, 2021. http://hdl.handle.net/10342/6929.

MLA Handbook (7^th Edition):

Angermeier, Tori Marena. “Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers.” 2018. Web. 04 Mar 2021.

Vancouver:

Angermeier TM. Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers. [Internet] [Masters thesis]. East Carolina University; 2018. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10342/6929.

Council of Science Editors:

Angermeier TM. Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers. [Masters Thesis]. East Carolina University; 2018. Available from: http://hdl.handle.net/10342/6929

Hong Kong University of Science and Technology

25. Wu, Yicong. Autofluorescence spectroscopy of epithelial tissue.

Degree: 2006, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-2854 ; https://doi.org/10.14711/thesis-b938211 ; http://repository.ust.hk/ir/bitstream/1783.1-2854/1/th_redirect.html

► Fluorescence diagnosis of epithelial precancer is based on the difference in spectral characteristics between normal and precancerous tissue. However, the bulk autofluorescence measured by conventional… (more)

▼ Fluorescence diagnosis of epithelial precancer is based on the difference in spectral characteristics between normal and precancerous tissue. However, the bulk autofluorescence measured by conventional methods do not provide accurate diagnostic information such as structure and metabolism of tissue because the signal is a mixture of the fluorescence from different tissue layers. In this study, we demonstrated that depth-resolved fluorescence measurement can isolate the fluorescence signals from different tissue layers and provide more accurate information for tissue diagnosis. We instrumented a confocal fluorescence spectroscopy system with single-photon excitation from 355 - 473 nm to investigate the layered structure and biochemistry of various epithelial tissues. It was found that strong keratin fluorescence from the keratinized epithelial layer creates severe interference in the assessment of NADH, FAD and collagen fluorescence. The depth-resolved fluorescence spectra excited at 355 nm produced sufficient contrast to resolve such fine structure, and the depth-resolved redox ratio from non-keratinized epithelium (the ratio of NADH fluorescence excited at 355nm over FAD fluorescence excited at 457nm) showed high correlation with tissue pathology. Furthermore, we demonstrated that confocal time-resolved measurement with single excitation from uv to violet can potentially provide accurate information for tissue diagnosis because different tissue layers exhibited different fluorescence time decays and the time decays of epithelial fluorescence were sensitive indicators of cellular metabolism. The depth-resolved fluorescence measurement was also achieved using two-photon excitation from 710 - 810 nm. The two-photon excited fluorescence (TPEF) signals from the keratinized epithelial layer, non-keratinized epithelium and underlying stroma exhibited different spectral characteristics providing information on biomorphology and biochemistry of epithelial tissue. The second harmonic generation (SHG) signals served as a sensitive indicator of collagen to separate the epithelial layer from underlying stroma. The results also demonstrated the potential of depth-resolved TPEF and SHG in determining the pathology of epithelial tissue.

Subjects/Keywords: Epithelium ; Fluorescence spectroscopy

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APA (6^th Edition):

Wu, Y. (2006). Autofluorescence spectroscopy of epithelial tissue. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-2854 ; https://doi.org/10.14711/thesis-b938211 ; http://repository.ust.hk/ir/bitstream/1783.1-2854/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Yicong. “Autofluorescence spectroscopy of epithelial tissue.” 2006. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021. http://repository.ust.hk/ir/Record/1783.1-2854 ; https://doi.org/10.14711/thesis-b938211 ; http://repository.ust.hk/ir/bitstream/1783.1-2854/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Yicong. “Autofluorescence spectroscopy of epithelial tissue.” 2006. Web. 04 Mar 2021.

Vancouver:

Wu Y. Autofluorescence spectroscopy of epithelial tissue. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2006. [cited 2021 Mar 04]. Available from: http://repository.ust.hk/ir/Record/1783.1-2854 ; https://doi.org/10.14711/thesis-b938211 ; http://repository.ust.hk/ir/bitstream/1783.1-2854/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu Y. Autofluorescence spectroscopy of epithelial tissue. [Thesis]. Hong Kong University of Science and Technology; 2006. Available from: http://repository.ust.hk/ir/Record/1783.1-2854 ; https://doi.org/10.14711/thesis-b938211 ; http://repository.ust.hk/ir/bitstream/1783.1-2854/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

26. Zhao, Yueyue CHEM. Fluorescent molecules with aggregation-induced emission characteristics in biomedical applications.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-100444 ; https://doi.org/10.14711/thesis-b1626224 ; http://repository.ust.hk/ir/bitstream/1783.1-100444/1/th_redirect.html

► Fluorescent imaging is one of the most rapidly expanding research area in biological science. As the high demand of new technologies in fluorescence, the fluorescent… (more)

▼ Fluorescent imaging is one of the most rapidly expanding research area in biological science. As the high demand of new technologies in fluorescence, the fluorescent materials have been taken to the central stage due to their unique optical properties, low background noise, and good processibility. The development of fluorescent bioprobes with excellent sensitivity, specificity, and biocompatibility is highly desirable because it allows on-site detection of biological macromolecules and real-time monitoring of biological events in living systems. Nevertheless, most of the conventional organic fluorophores suffer from a severe problem of aggregation-caused quenching (ACQ) at high concentration or in the aggregated state, resulting in great limitations in the biological applications. Recently, our group discovered such a novel system, in which fluorogen aggregation plays a constructive, instead of destructive, role in the light-emitting process. We coined this abnormal phenomenon as “aggregation-induced emission” (AIE), and identified the restriction of intramolecular rotation (RIR) as the main cause of the AIE effect. Thanks to the enthusiastic effort of scientists working in AIE research, many AIE materials with different structures and emission colors have been developed. The high brightness of AIE materials in the aggregated state makes them promising candidate materials as fluorescent bioprobes for sensing and imaging applications. In this work, a series of new AIE fluorogens have been designed and synthesized. They are weakly emissive in water but emit intensely when their intramolecular rotations have been restricted upon binding to the specific sites in cells. Such a light-up feature of fluorescent probe enables the quantitation and visualization of research of interest in cells. Moreover, these AIE probes do not only serve as fluorescent visualizers for fast intracellular imaging, they also serve as therapeutic agents for cancer treatment. The AIE bioprobes can be utilized as a long-term tracer for monitoring the drug distribution and responses.

Subjects/Keywords: Fluorescence spectroscopy ; Fluorescent polymers ; Biofluorescence ; Aggregation (Chemistry)

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APA (6^th Edition):

Zhao, Y. C. (2016). Fluorescent molecules with aggregation-induced emission characteristics in biomedical applications. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-100444 ; https://doi.org/10.14711/thesis-b1626224 ; http://repository.ust.hk/ir/bitstream/1783.1-100444/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhao, Yueyue CHEM. “Fluorescent molecules with aggregation-induced emission characteristics in biomedical applications.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021. http://repository.ust.hk/ir/Record/1783.1-100444 ; https://doi.org/10.14711/thesis-b1626224 ; http://repository.ust.hk/ir/bitstream/1783.1-100444/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhao, Yueyue CHEM. “Fluorescent molecules with aggregation-induced emission characteristics in biomedical applications.” 2016. Web. 04 Mar 2021.

Vancouver:

Zhao YC. Fluorescent molecules with aggregation-induced emission characteristics in biomedical applications. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 04]. Available from: http://repository.ust.hk/ir/Record/1783.1-100444 ; https://doi.org/10.14711/thesis-b1626224 ; http://repository.ust.hk/ir/bitstream/1783.1-100444/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhao YC. Fluorescent molecules with aggregation-induced emission characteristics in biomedical applications. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-100444 ; https://doi.org/10.14711/thesis-b1626224 ; http://repository.ust.hk/ir/bitstream/1783.1-100444/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

National University of Ireland – Galway

27. Groza, Radu Constantin. Anisotropy resolved multi-dimensional emission spectroscopy (ARMES): a new tool for the quantitative and structural analysis of proteins .

Degree: 2016, National University of Ireland – Galway

URL: http://hdl.handle.net/10379/5645

► The quantitative and structural analysis of proteins is of great importance in numerous fields (from industrial to academic research). Requirements like ease of analysis, sample… (more)

▼ The quantitative and structural analysis of proteins is of great importance in numerous fields (from industrial to academic research). Requirements like ease of analysis, sample handling, robustness, analysis time, the amount of analytical information provided and affordability may all be essential and must be taken into account when developing any analytical method. Fluorescence spectroscopy is a highly attractive method that can fulfill many of these requirements and has an ever-increasing number of applications aimed towards the study of proteins, based either on the use of intrinsic fluorescence or extrinsic labels. One of the most important industrial sectors where new protein analysis techniques are in great demand is the BioPharma manufacturing industry. A typical bioprocess consists of growing cells or cell components (predominantly mammalian cells) in culture media (complex and often ill-defined mixtures) in order to produce recombinant therapeutic proteins. During these processes, proteins not only represent the final product (i.e. glycoproteins), but are also often used as additives (i.e. albumins, insulins) in some, if not most, culture media. Therefore, in many parts of the bioprocess it is vitally important to be able to measure both protein concentration and stability. This is especially challenging when the matrix (i.e. cell culture media or bioprocess broth) in which the protein is located is chemically complex. The first part of this thesis research was based on developing new fluorescence based methods for the quantitative analysis of proteins in highly complex biogenic mixtures (which are themselves intrinsically fluorescent). Due to the complexity of such mixtures, the main issue was the unambiguous discrimination of protein emission from that of free amino acids present in the media. Anisotropy/polarization was the method of emission discrimination used and was integrated into multi-dimensional fluorescence (MDF) measurements (Chapter 3). This new method enabled the protein signal to be isolated based on the protein’s size compared to the free amino acids in media and enabled accurate protein concentration measurements (Chapter 4). The MDF anisotropy spectra of albumin solutions showed a striped pattern that was a unique feature of multi-fluorophore proteins. We also noticed that these MDF anisotropy patterns changed differently depending on the specific unfolding pathway. The emission of albumins is complicated because of the multiple fluorophores present (Trp, Tyr, and to a lesser extent Phe). Combining anisotropy with MDF and chemometric data analysis allowed us to resolve emission from groups of Tyr and Trp and obtain a more in-depth understanding of the structural changes that occur compared to conventional fluorescence spectroscopy. Chemometric data analysis was used to extract the contributions of individual emitting fluorophores to the overall anisotropy pattern changes of human serum albumin during thermal and chemical unfolding. This new method was called Anisotropy Resolved… Advisors/Committee Members: Ryder, Alan G (advisor).

Subjects/Keywords: Spectroscopy; Chemometrics; Protein analysis; Fluorescence; Anisotropy; Chemistry

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APA (6^th Edition):

Groza, R. C. (2016). Anisotropy resolved multi-dimensional emission spectroscopy (ARMES): a new tool for the quantitative and structural analysis of proteins . (Thesis). National University of Ireland – Galway. Retrieved from http://hdl.handle.net/10379/5645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Groza, Radu Constantin. “Anisotropy resolved multi-dimensional emission spectroscopy (ARMES): a new tool for the quantitative and structural analysis of proteins .” 2016. Thesis, National University of Ireland – Galway. Accessed March 04, 2021. http://hdl.handle.net/10379/5645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Groza, Radu Constantin. “Anisotropy resolved multi-dimensional emission spectroscopy (ARMES): a new tool for the quantitative and structural analysis of proteins .” 2016. Web. 04 Mar 2021.

Vancouver:

Groza RC. Anisotropy resolved multi-dimensional emission spectroscopy (ARMES): a new tool for the quantitative and structural analysis of proteins . [Internet] [Thesis]. National University of Ireland – Galway; 2016. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10379/5645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Groza RC. Anisotropy resolved multi-dimensional emission spectroscopy (ARMES): a new tool for the quantitative and structural analysis of proteins . [Thesis]. National University of Ireland – Galway; 2016. Available from: http://hdl.handle.net/10379/5645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

28. Liu, Peng. The study of two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper raman and surface-enhanced resonance hyper raman spectroscopy.

Degree: 2011, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7292 ; https://doi.org/10.14711/thesis-b1155284 ; http://repository.ust.hk/ir/bitstream/1783.1-7292/1/th_redirect.html

► The objective of this thesis is to study the two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper Raman (RHRS) and surface-enhanced resonance hyper… (more)

▼ The objective of this thesis is to study the two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper Raman (RHRS) and surface-enhanced resonance hyper Raman spectroscopy (SERHRS). These excited states were believed to dictate the nonlinear optical (NLO) properties of octupolar dyes. Specifically, we have systematically studied two classes of octupolar molecules, organic dyes and transition metal complexes, respectively, by RHRS and/or SERHRS. The organic octupolar dyes were chosen because of their wide applications as optical imaging probes in biological sciences. The octupolar transition metal complexes were chosen because of their extensive use as photosensitizers in solar energy conversion. The ultimate goals of this thesis are to provide guidance for the design and synthesis of new NLO chromophoric molecules, and to pave the way for the development of NLO imaging techniques based on multi-photon excited hyper Raman scattering. Chapter 1 gives a concise introduction and overview of Raman scattering and hyper Raman scattering under normal, resonance-enhanced, and surface-enhanced conditions. The basic and general properties of octupolar molecules are also outlined. Chapters 2 to 4 report a systematic study of three organic octupolar dyes, crystal violet (CV), basic fuchsin (BF), and a triazine dye (T72), by both Raman and hyper Raman spectroscopy under normal, resonance-enhanced and surface-enhanced conditions. Raman excitation profiles (REPs) and hyper Raman excitation profiles (HREPs) were measured to establish the vibronic nature of the excited states. DFT and TD-DFT calculations were carried out to assign the normal modes of the molecules as well as the excited vibronic states involved. Two intensity calibration methods for SERHRS were developed, one of which uses pyridine as the external reference standard and another uses dissolved Na2MoO4 as the internal reference standard. Both methods were validated by testing on CV in the presence and absence of surface enhancement. The most important discoveries include (a) the revelation of two-photon-allowed excited states by HREPs, and (b) observation of multiplexed non-linear multiphoton scattering by single color excitation. Chapters 5 to 7 report a systematic study of three octupolar transition metal complexes [M(bpy)3]2+ (bpy = 2,2′-bipyridine, M = RuII, OsII and FeII) by Raman and hyper Raman spectroscopy under surface-enhanced resonance conditions. The main discovery is the observation of the two-photon-allowed but one-photon forbidden metal-to-ligand charge transfer (MLCT) excited state, revealed by HREPs, which could not be observed by conventional linear optical techniques. DFT and TD-DFT calculations were performed to assign the normal modes of vibration, as well as the excited states involved in the observed HREPs. To the best of my knowledge, this thesis reports hitherto the most extensive study of both organic and inorganic octupolar molecules by two-photon excited hyper Raman spectroscopy under resonance and surface…

Subjects/Keywords: Raman spectroscopy ; Nonlinear optics ; Fluorescence microscopy

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APA (6^th Edition):

Liu, P. (2011). The study of two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper raman and surface-enhanced resonance hyper raman spectroscopy. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7292 ; https://doi.org/10.14711/thesis-b1155284 ; http://repository.ust.hk/ir/bitstream/1783.1-7292/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Peng. “The study of two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper raman and surface-enhanced resonance hyper raman spectroscopy.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021. http://repository.ust.hk/ir/Record/1783.1-7292 ; https://doi.org/10.14711/thesis-b1155284 ; http://repository.ust.hk/ir/bitstream/1783.1-7292/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Peng. “The study of two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper raman and surface-enhanced resonance hyper raman spectroscopy.” 2011. Web. 04 Mar 2021.

Vancouver:

Liu P. The study of two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper raman and surface-enhanced resonance hyper raman spectroscopy. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Mar 04]. Available from: http://repository.ust.hk/ir/Record/1783.1-7292 ; https://doi.org/10.14711/thesis-b1155284 ; http://repository.ust.hk/ir/bitstream/1783.1-7292/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu P. The study of two-photon-allowed excited states of octupolar chromophoric molecules by resonance hyper raman and surface-enhanced resonance hyper raman spectroscopy. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7292 ; https://doi.org/10.14711/thesis-b1155284 ; http://repository.ust.hk/ir/bitstream/1783.1-7292/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Amankona, Boniface Osei. Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy.

Degree: MS, Chemistry, 2018, Encompass Digital Archive, Eastern Kentucky University

URL: https://encompass.eku.edu/etd/502

► Atrazine is the most widely used herbicides in USA, but its negative impact on aquatic ecosystem cannot be over-looked. Atrazine was traditionally determined through… (more)

Subjects/Keywords: Atrazine; Fluorescence; Fluorolog; Freshwater; Spectroscopy; Organic Chemistry

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APA (6^th Edition):

Amankona, B. O. (2018). Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy. (Masters Thesis). Encompass Digital Archive, Eastern Kentucky University. Retrieved from https://encompass.eku.edu/etd/502

Chicago Manual of Style (16^th Edition):

Amankona, Boniface Osei. “Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy.” 2018. Masters Thesis, Encompass Digital Archive, Eastern Kentucky University. Accessed March 04, 2021. https://encompass.eku.edu/etd/502.

MLA Handbook (7^th Edition):

Amankona, Boniface Osei. “Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy.” 2018. Web. 04 Mar 2021.

Vancouver:

Amankona BO. Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy. [Internet] [Masters thesis]. Encompass Digital Archive, Eastern Kentucky University; 2018. [cited 2021 Mar 04]. Available from: https://encompass.eku.edu/etd/502.

Council of Science Editors:

Amankona BO. Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy. [Masters Thesis]. Encompass Digital Archive, Eastern Kentucky University; 2018. Available from: https://encompass.eku.edu/etd/502

Princeton University

30. Taffet, Elliot. Unmasking Underlying Molecular Photophysical Mechanisms Using Quantum Chemistry .

Degree: PhD, 2019, Princeton University

URL: http://arks.princeton.edu/ark:/88435/dsp017w62fc131

► Quantum chemistry is performed on biological chromophores in light harvesting and synthetic chromophores in targeted photochemical applications to elucidate how molecular electronic structure impacts their… (more)

Subjects/Keywords: exchange; fission; fluorescence; multireference; photophysics; spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Taffet, E. (2019). Unmasking Underlying Molecular Photophysical Mechanisms Using Quantum Chemistry . (Doctoral Dissertation). Princeton University. Retrieved from http://arks.princeton.edu/ark:/88435/dsp017w62fc131

Chicago Manual of Style (16^th Edition):

Taffet, Elliot. “Unmasking Underlying Molecular Photophysical Mechanisms Using Quantum Chemistry .” 2019. Doctoral Dissertation, Princeton University. Accessed March 04, 2021. http://arks.princeton.edu/ark:/88435/dsp017w62fc131.

MLA Handbook (7^th Edition):

Taffet, Elliot. “Unmasking Underlying Molecular Photophysical Mechanisms Using Quantum Chemistry .” 2019. Web. 04 Mar 2021.

Vancouver:

Taffet E. Unmasking Underlying Molecular Photophysical Mechanisms Using Quantum Chemistry . [Internet] [Doctoral dissertation]. Princeton University; 2019. [cited 2021 Mar 04]. Available from: http://arks.princeton.edu/ark:/88435/dsp017w62fc131.

Council of Science Editors:

Taffet E. Unmasking Underlying Molecular Photophysical Mechanisms Using Quantum Chemistry . [Doctoral Dissertation]. Princeton University; 2019. Available from: http://arks.princeton.edu/ark:/88435/dsp017w62fc131

Open Access Theses and Dissertations