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You searched for subject:(Flock PRNP genotyping). One record found.

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Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

1. Psifidi, Androniki. Προσδιορισμός των γενότυπων προβάτων ως προς το γονίδιο της τρομώδους νόσου (scrapie) με βάση το DNA των σωματικών κυττάρων του γάλατος και σχέση μεταξύ γενότυπων και ζωοτεχνικών χαρακτηριστικών.

Degree: 2010, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

1.013 individual ovine milk samples were collected from as many purebred Chios ewes raised in 23 flocks-members of the national Chios Sheep Breeder’s Cooperative. Genomic DNA was extracted from milk somatic cells using a modified commercially available extraction kit, optimised for ovine milk. Genotyping at codons 136, 154, and 171 (classical scrapie) and 141 (atypical scrapie) was performed using a PCR- RFLP analysis protocol. A total of 15 genotypes and 6 alleles (ARQ, AHQ, TRQ, ARR, ARH, VRQ) linked to codons 136, 154 and 171 were detected. Allelic frequencies were 76.1%, 8.2%, 7.7%, 6.9%, 0.7% and 0.4% for ARQ, AHQ, TRQ, ARR, ARH and VRQ, respectively. All animals were homozygous for the leucine allele at codon 141. Αrtificial bulk milk samples were created in the lab by mixing individual milk samples from ewes with known genotypes. Genomic DNA was extracted from these samples using the Modified blood kit. PRNP genotyping was performed in the artificial bulk milk samples using a similar PCR-RFLP analysis protocol. In addition, standard samples were constructed by plasmid DNA and equimolar DNA extracts in order to determine the detection limit (LOD) of each PRNP allele with RFLP analysis. Results showed that LOD of VRQ and AHQ is 0.5%, ARR and ARH is 4% and TRQ is 8%. Furthermore, the impact of the PRNP gene locus on milk production (test-day and total lactation yield) and reproduction (age at first lambing, conception rate at first service and prolificacy) of Chios sheep was assessed using mixed linear models. The association studies were based on records obtained from the official dataset of Chios Sheep Breeder’s Cooperative «Macedonia». The ARQ allele was found to have a favourable effect on conception rate at first service and age at first lambing. The TRQ allele, whose association with such traits was assessed for the first time, had a positive effect on test-day milk yield and an adverse effect on age at first lambing. Nevertheless, only the effect of TRQ on age at first lambing remained statistical significant after the correction with Bonferroni test. The ARR allele, which is associated with increased resistance to classical scrapie, had no significant effect on any of the traits studied. No significant associations of the PRNP genotypes with production and reproduction traits were observed. Selection for scrapie-resistant sheep is not expected to affect milk yield and reproduction of the Chios breed. Moreover, the proposed PCR-RFLP method, using bulk milk, can provide a useful test for fast screening of flocks for the presence of undesirable alleles such as VRQ or AHQ. In case, for example, that VRQ-carriers are detected in a flock, individual animal genotyping could then be performed to exclude these undesirable animals from flocks. In addition, milk somatic cells are a practical, animal friendly source of genomic DNA which could be used in order to facilitate the sampling for individual PRNP genotyping in a large scale application.

Από 1.013 προβατίνες φυλής Χίου, που εκτρέφονταν σε 23 ποίμνια,…

Subjects/Keywords: Τρομώδης νόσος; Γονίδιο πρωτεΐνης Prion; Πρόβατα φυλής Χίου; Απομόνωση DNA από τα σωματικά κύτταρα του γάλατος; Αλυσιδωτή αντίδραση πολυμεράσης; Ανάλυση πολυμορφισμού του μήκους περιοριστικών θραυσμάτων; Ομαδικός γενοτυπικός προσδιορισμός; Γαλακτοπαραγωγική και αναπαραγωγική ικανότητα; Scrapie; PRNP gene; Chios sheep; DNA extraction from milk somatic cells; Polymerase chain reaction (PCR); Restriction fragment length polymorphism (RFLP); Flock PRNP genotyping; Milk production and reproduction

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APA (6th Edition):

Psifidi, A. (2010). Προσδιορισμός των γενότυπων προβάτων ως προς το γονίδιο της τρομώδους νόσου (scrapie) με βάση το DNA των σωματικών κυττάρων του γάλατος και σχέση μεταξύ γενότυπων και ζωοτεχνικών χαρακτηριστικών. (Thesis). Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/25630

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Psifidi, Androniki. “Προσδιορισμός των γενότυπων προβάτων ως προς το γονίδιο της τρομώδους νόσου (scrapie) με βάση το DNA των σωματικών κυττάρων του γάλατος και σχέση μεταξύ γενότυπων και ζωοτεχνικών χαρακτηριστικών.” 2010. Thesis, Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ). Accessed November 29, 2020. http://hdl.handle.net/10442/hedi/25630.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Psifidi, Androniki. “Προσδιορισμός των γενότυπων προβάτων ως προς το γονίδιο της τρομώδους νόσου (scrapie) με βάση το DNA των σωματικών κυττάρων του γάλατος και σχέση μεταξύ γενότυπων και ζωοτεχνικών χαρακτηριστικών.” 2010. Web. 29 Nov 2020.

Vancouver:

Psifidi A. Προσδιορισμός των γενότυπων προβάτων ως προς το γονίδιο της τρομώδους νόσου (scrapie) με βάση το DNA των σωματικών κυττάρων του γάλατος και σχέση μεταξύ γενότυπων και ζωοτεχνικών χαρακτηριστικών. [Internet] [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2010. [cited 2020 Nov 29]. Available from: http://hdl.handle.net/10442/hedi/25630.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Psifidi A. Προσδιορισμός των γενότυπων προβάτων ως προς το γονίδιο της τρομώδους νόσου (scrapie) με βάση το DNA των σωματικών κυττάρων του γάλατος και σχέση μεταξύ γενότυπων και ζωοτεχνικών χαρακτηριστικών. [Thesis]. Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ); 2010. Available from: http://hdl.handle.net/10442/hedi/25630

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.