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You searched for subject:(Estrogen deprivation). Showing records 1 – 2 of 2 total matches.

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Dalhousie University

1. Wibowo, Erik. MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY.

Degree: PhD, Department of Medical Neuroscience, 2013, Dalhousie University

Advanced prostate cancer (PCa) patients are offered androgen deprivation therapy (ADT) to control their cancer’s growth. ADT impairs sexual function and the sleep patterns of ADT patients. Since ADT deprives patients of estrogen, and supplemental estrogen reduces such problems in menopausal women, I studied whether administering estrogen reduces these problems for castrated male rats as a model for PCa patients on ADT. First, I tested how early versus late estradiol treatment after castration influenced rats’ sexual behaviour. Estradiol increases mounting behaviour to comparable levels regardless of when the treatment was started after castration, suggesting that estrogen’s ability to restore male sexual interest is insensitive to a delay since castration. Secondly, to understand the biological basis of these behavioural effects, I examined brain and muscle tissues from the same animals. Specifically, I compared changes in 1) estrogen receptors (ERs) and c-Fos protein (a neuronal activation marker) levels in brain areas controlling sex behavior; 2) ERs levels in pelvic floor muscles, important for erection; and 3) ERs levels in the hippocampus and prefrontal cortex. Prolonged castration increases ER? levels in the preoptic area (POA), a key brain area that regulates mating behaviour, and estradiol treatment reduced these effects. In the POA, mating-induced c-Fos expression was not affected by estradiol regardless of when the treatment began post-castration. Estrogen may upregulate ERs in pelvic floor muscles, and downregulate ERs in the hippocampus and prefrontal cortex, depending on administration time after castration. These findings suggest that mating activates POA neurons, and this activation induces mounting only in the presence of estrogen. Additionally, the duration after castration influences ER autoregulation in the pelvic floor muscles, hippocampus, and prefrontal cortex in response to estradiol. Lastly, I studied how estrogen modulates the sleep-wake behaviour of orchiectomized rats. Estradiol promotes baseline wakefulness during the dark period and prevents castration-induced impairment in sleep recovery after sleep deprivation. These findings suggest that estradiol may positively influence the sleep-wake behaviour of castrated males. Collectively, I demonstrate that estrogen administered to castrated rats improves sexual and sleep functions. It may similarly improve the quality of life of PCa patients on ADT. Advisors/Committee Members: Dr. Jessica Mong (external-examiner), Dr. Kazue Semba (graduate-coordinator), Dr. Tara Perrot, Dr. Richard Brown (thesis-reader), Dr. Kazue Semba, Dr. Richard Wassersug (thesis-supervisor), Received (ethics-approval), Yes (manuscripts), Yes (copyright-release).

Subjects/Keywords: Prostate Cancer; Androgen Deprivation Therapy; Estrogen; Sexual Interest; Sleep; Estrogen Receptors; Pelvic Floor Muscles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Wibowo, E. (2013). MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY. (Doctoral Dissertation). Dalhousie University. Retrieved from http://hdl.handle.net/10222/36281

Chicago Manual of Style (16th Edition):

Wibowo, Erik. “MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY.” 2013. Doctoral Dissertation, Dalhousie University. Accessed September 23, 2019. http://hdl.handle.net/10222/36281.

MLA Handbook (7th Edition):

Wibowo, Erik. “MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY.” 2013. Web. 23 Sep 2019.

Vancouver:

Wibowo E. MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY. [Internet] [Doctoral dissertation]. Dalhousie University; 2013. [cited 2019 Sep 23]. Available from: http://hdl.handle.net/10222/36281.

Council of Science Editors:

Wibowo E. MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY. [Doctoral Dissertation]. Dalhousie University; 2013. Available from: http://hdl.handle.net/10222/36281


Universidade do Rio Grande do Sul

2. Miragem, Antônio Azambuja. Efeito agudo do tratamento térmico nos níveis de HSP70 e marcadores de estresse oxidativo de ratas wistar.

Degree: 2015, Universidade do Rio Grande do Sul

Os fogachos são a queixa mais comum das mulheres peri- e pós-menopausa e estão fortemente relacionados com a diminuição dos níveis de estrogênio. No entanto, a fisiopatologia deste sintoma vasomotor muito desagradável é ainda desconhecido. Por outro lado, o estradiol (E2) apresenta a capacidade de induzir a expressão de HSP72, um membro da família de 70 kDa das proteínas de choque térmico (HSP70), que são citoprotetoras e cardioprotetoras. Sabe-se que a expressão HSP70 é comprometida em doenças inflamatórias relacionadas com o envelhecimento. Por isso, questionamos se a capacidade do organismo de desencadear uma resposta ao choque térmico (Heat Shock Response) robusta, estaria ainda presente após a retirada, via castração, do E2. Para tanto, foram estudados os efeitos do choque térmico (Heat Shock - HS), através de uma sessão de banho de imersão, em ratas Wistar submetidas a ovariectomia bilateral (OVX), após um período de washout hormonal de 7 dias. Doze horas após o HS, os animais foram mortos e o arco aórtico foi excisado cirurgicamente para análises moleculares. Os resultados foram comparados com os marcadores de estresse oxidativo no plasma (superóxido dismutase, catalase e lipoperoxidação), pois é bem estabelecido que a expressão de HSP70 é sensível a regulação redox. A relação entre a iHSP70 (intracelular) e a eHSP70 (extracelular/plasma), proposto como um índice do estado inflamatório sistêmico, também foi investigada. Os resultados mostraram que a Heat Shock Response continua preservada em animais OVX, como inferido a partir da expressão de HSP70 (até 40% de aumento, p <0,01) nas aortas, o que não foi acompanhado por nenhuma outra alteração em marcadores de estresse oxidativo, parâmetros hematológicos e no controle glicêmico. Desta forma, sugerimos que a avaliação periódica do status de HSP70 (iHSP70 vs eHSP70) pode ser de extrema relevância clínica, pois a diminuição da capacidade de defesa do organismo via Heat Shock Response está no centro do aparecimento de disfunções relacionadas com a menopausa.

Hot flashes, the most common complaint of peri- and postmenopausal women, are tightly related to decrease in estrogen levels. However, the pathophysiology of this very unpleasant vasomotor symptom is greatly unknown. On the other hand, estradiol (E2) has been found to induce the expression of HSP72, a member of the 70 kDa family of heat shock proteins (HSP70), which are cytoprotective and cardioprotective. Since it has been noticed that HSP70 expression is compromised in age-related inflammatory diseases, we argued whether the capacity of triggering a robust heat shock (HS) response would be still present after E2 withdrawal. Hence, we studied the effects of HS treatment (hot tub) in female Wistar rats subjected to bilateral oophorectomy (OVX) after a 7 day washout period. Twelve hours after HS, the animals were killed and aortic arches were surgically excised for molecular analyses. The results were compared with oxidative stress markers in the plasma (superoxide dismutase, catalase and lipoperoxidation)…

Advisors/Committee Members: Bittencourt Junior, Paulo Ivo Homem de.

Subjects/Keywords: Proteínas de choque térmico HSP70; Heat shock proteins; Estresse oxidativo; HSP70; Estrogen deprivation; Biomarcadores; Menopausa; Menopause; Stress response; Fogachos; Estrogênios

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Miragem, A. A. (2015). Efeito agudo do tratamento térmico nos níveis de HSP70 e marcadores de estresse oxidativo de ratas wistar. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/127427

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Miragem, Antônio Azambuja. “Efeito agudo do tratamento térmico nos níveis de HSP70 e marcadores de estresse oxidativo de ratas wistar.” 2015. Thesis, Universidade do Rio Grande do Sul. Accessed September 23, 2019. http://hdl.handle.net/10183/127427.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Miragem, Antônio Azambuja. “Efeito agudo do tratamento térmico nos níveis de HSP70 e marcadores de estresse oxidativo de ratas wistar.” 2015. Web. 23 Sep 2019.

Vancouver:

Miragem AA. Efeito agudo do tratamento térmico nos níveis de HSP70 e marcadores de estresse oxidativo de ratas wistar. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2015. [cited 2019 Sep 23]. Available from: http://hdl.handle.net/10183/127427.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miragem AA. Efeito agudo do tratamento térmico nos níveis de HSP70 e marcadores de estresse oxidativo de ratas wistar. [Thesis]. Universidade do Rio Grande do Sul; 2015. Available from: http://hdl.handle.net/10183/127427

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.