subject:(Estrogen Receptors)

Rutgers University

1. Yasrebi, Ali, 1987-. ERE-independent ERα signalling in feeding and exploratory behaviors.

Degree: MS, Endocrinology and Animal Biosciences, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/62067/

► The reproductive steroid hormone, 17β-estradiol (E2), controls feeding and exploratory behaviors associated with mood disorders. The loss of circulating E2 puts menopausal women at an… (more)

▼ The reproductive steroid hormone, 17β-estradiol (E2), controls feeding and exploratory behaviors associated with mood disorders. The loss of circulating E2 puts menopausal women at an increased risk for developing obesity and mood disorders when compared to premenopausal women. Therefore, it is critically important to understand the role of sex steroids and their receptors in the neuroendocrine control of feeding and mood. The goal of this project is to understand the role of estrogen response Element (ERE)-dependent and ERE-independent ERα signaling on behavior by characterizing feeding patters and exploratory behaviors in male and female mice lacking either total ERα signaling or lacking ERE-dependent ERα signaling. We hypothesize that ERE-independent ERα is partially sufficient to restore feeding and exploratory behaviors that are lost in total ERα knockout mice. We tested three strains of mice: two ERα transgenic models, a total ERα knock out (ERKO) and a novel ERα knock in/knock out (KIKO) that lacks a functional DNA-binding domain) and their wild type (WT) C57 littermates using a real-time feeding behavior monitoring system and series of standard behavior tests (open field tests, elevated plus maze, forced swim test). To test our hypothesis FI and meal patterns were observed while the animals were given ad libitum access to a LFD (Experiment 1a) followed by HFD (Experiment 1b). A separate set of animals the response to fasting was monitored for 24 h after caloric restriction (Experiment 1c). Exploratory, depressive, and locomotor behavior testing was conducted on mice from Experiments 1a/b (open field, elevated plus maze, forced swim test). Each experiment was initially done with intact animals and then again repeated in ovariectomized (OVX) animals split into either an oil treated control group or an E2-treated group. We observed ERE-dependent mechanisms are the main modulator of homeostatic LFD feeding meal patterns while ERE- independent ERα signaling was involved in the control of palatable, high-fat diet food intake. During refeeding, ERE-independent mechanisms contribute to a decreased first meal food intake and slower rate of ingestion. When observed during a series of behavior tests, WT animals explored more, regardless of treatment (differences could be attributed to higher levels of locomotor activity in WT). However, similarities between WT and KIKO females in the EPM indicate that ERE-independent pathways may contribute towards reducing anxiety measures, independent of locomotor activity. WT females were shown to have a decreased free float time, indicating ERE dependent signaling may be influencing despair like tendencies. Collectively, these suggest that both ERE-dependent and -independent ERαsignaling are involved with both feeding and anxiety like homeostatic parameters. Advisors/Committee Members: Roepke, Troy A (chair), Bello, Nicholas T (internal member), Bagnell, Carol A (internal member), Samuels, Benjamin A (outside member), School of Graduate Studies.

Subjects/Keywords: Estrogen receptor alpha; Estrogen – Receptors

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APA (6^th Edition):

Yasrebi, Ali, 1. (2019). ERE-independent ERα signalling in feeding and exploratory behaviors. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/62067/

Chicago Manual of Style (16^th Edition):

Yasrebi, Ali, 1987-. “ERE-independent ERα signalling in feeding and exploratory behaviors.” 2019. Masters Thesis, Rutgers University. Accessed January 25, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/62067/.

MLA Handbook (7^th Edition):

Yasrebi, Ali, 1987-. “ERE-independent ERα signalling in feeding and exploratory behaviors.” 2019. Web. 25 Jan 2021.

Vancouver:

Yasrebi, Ali 1. ERE-independent ERα signalling in feeding and exploratory behaviors. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Jan 25]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/62067/.

Council of Science Editors:

Yasrebi, Ali 1. ERE-independent ERα signalling in feeding and exploratory behaviors. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/62067/

2. Shen, Minqian. Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism.

Degree: PhD, Cell, Molecular and Structural Biology (CMSB), 2017, Miami University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921

► Liver is one of the most essential organs involved in the regulation of energy homeostasis. Hepatic steatosis, a major manifestation of metabolic syndrome, is associated… (more)

Subjects/Keywords: Biology; Estrogen; Estrogen receptors; Liver; HepG2; Obesity

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APA (6^th Edition):

Shen, M. (2017). Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism. (Doctoral Dissertation). Miami University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921

Chicago Manual of Style (16^th Edition):

Shen, Minqian. “Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism.” 2017. Doctoral Dissertation, Miami University. Accessed January 25, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921.

MLA Handbook (7^th Edition):

Shen, Minqian. “Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism.” 2017. Web. 25 Jan 2021.

Vancouver:

Shen M. Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism. [Internet] [Doctoral dissertation]. Miami University; 2017. [cited 2021 Jan 25]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921.

Council of Science Editors:

Shen M. Roles of estrogen hormones and estrogen receptors on regulation of liver and liver cancer metabolism. [Doctoral Dissertation]. Miami University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1492612390075921

University of Houston

3. Jonsson, Philip 1985-. Transcriptional Control of Oncogenic Processes in Breast Cancer Cells by the Estrogen Receptors.

Degree: PhD, Biology, 2014, University of Houston

URL: http://hdl.handle.net/10657/1949

► The estrogen receptors are fundamental factors in human biology. As transcriptional factors regulating gene programs controlling many processes in the body, they are key in… (more)

▼ The estrogen receptors are fundamental factors in human biology. As transcriptional factors regulating gene programs controlling many processes in the body, they are key in both development and disease. Also, as nuclear receptors they can be activated or blocked by specific ligands, making them excellent targets for therapeutics. This dissertation focuses on the study of the estrogen receptors, both the alpha and beta isoforms (ERα and ERβ, respectively), and how they regulate gene transcription in human breast cancer. The proliferative role of ERα in breast cancer remains poorly understood. Here we show that the ion channel KCNK5 is a direct transcriptional target of ERα in breast cancer cell lines MCF7 and T47D. Also, we show that this is reflected by changes in the ion channel’s protein. Furthermore, silencing of the ion channels expression reduces cellular proliferation, as well as the estrogen-induction of proliferation. This uncovers ion channels as potential factors in the proliferation of breast cancer, as well as potential targets in novel treatment approaches. ERα’s role as a transcription factor has predominantly been studied in regards to its regulation of protein-coding genes. Herein, we show that ERα also regulates non-coding RNAs, such as long non-coding RNAs and pseudogenes. We also potentially uncover novel protein-coding targets, by the use of novel RNA- sequencing technology, and the use of microarrays. The other estrogen receptor, ERβ, is less characterized, but it is considered to be anti-proliferative in breast cancer and its activation suggested as a potential future therapy. However, discordant results of expression in breast tumors, correlation to prognosis, and tumor-suppressive function in cell lines have made this a debated field. We explore its role in breast cancer cells further and show that, in certain contexts, ERβ is not able to suppress breast cancer cell proliferation, nor, as often suggested, counteract ERα-mediated signaling. This warrants further studies into whether its activation in breast cancer is a desirable treatment. Advisors/Committee Members: Williams, Cecilia M. (advisor), Gunaratne, Preethi H. (committee member), Webb, Paul (committee member), Willson, Richard C. (committee member).

Subjects/Keywords: Estrogen receptors; Breast cancer

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APA (6^th Edition):

Jonsson, P. 1. (2014). Transcriptional Control of Oncogenic Processes in Breast Cancer Cells by the Estrogen Receptors. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/1949

Chicago Manual of Style (16^th Edition):

Jonsson, Philip 1985-. “Transcriptional Control of Oncogenic Processes in Breast Cancer Cells by the Estrogen Receptors.” 2014. Doctoral Dissertation, University of Houston. Accessed January 25, 2021. http://hdl.handle.net/10657/1949.

MLA Handbook (7^th Edition):

Jonsson, Philip 1985-. “Transcriptional Control of Oncogenic Processes in Breast Cancer Cells by the Estrogen Receptors.” 2014. Web. 25 Jan 2021.

Vancouver:

Jonsson P1. Transcriptional Control of Oncogenic Processes in Breast Cancer Cells by the Estrogen Receptors. [Internet] [Doctoral dissertation]. University of Houston; 2014. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10657/1949.

Council of Science Editors:

Jonsson P1. Transcriptional Control of Oncogenic Processes in Breast Cancer Cells by the Estrogen Receptors. [Doctoral Dissertation]. University of Houston; 2014. Available from: http://hdl.handle.net/10657/1949

McGill University

4. Buell, Richard H. The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary Lesions.

Degree: PhD, Department of Pathology, 1984, McGill University

URL: https://escholarship.mcgill.ca/downloads/fb494b707.pdf ; https://escholarship.mcgill.ca/concern/theses/tb09j792p

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Le dosage biochimique des récepteurs ostrogéniques dans les adénocarcinomes mammaires humains a un intérêt clinique bien établi, cependant la localisation cellulaire des sites de liaison… (more)

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Le dosage biochimique des récepteurs ostrogéniques dans les adénocarcinomes mammaires humains a un intérêt clinique bien établi, cependant la localisation cellulaire des sites de liaison aux œstrogènes dans ces tumeurs demeure incertaine. Les travaux décrits dans cette thèse ont permis d'identifier les sites de liaison de l'oestradiol au moyen de l'autoradiographie après incubation in vitro dans une série de 57 cas de lésions mammaires dont 17 étaient bénignes et 40 étaient des adénocarcinomes. Les résultats obtenus avec la méthode d'incubation in vitro se comparent favorablement avec ceux obtenus dans des études in vivo portant sur l'utérus de souris, un organe cible des œstrogènes bien caractérise. Dans les lésions mammaires bénignes une proportion variable des cellules épithéliales contenaient des sites spécifiques de liaison ostrogénique. Les cellules myoépithéliales lorsqu'elles pouvaient être identifiées et les cellules stromales étaient en général négatives. Les adénocarcinomes mammaires avec un dosage de récepteur ostrogénique positif contenaient à la fois des cellules épithéliales marquées et des cellules négatives. Les résultats quantitatifs obtenus avec la méthode autoradiographique se comparent favorablement avec ceux obtenus avec les méthodes biochimiques.

The biochemical assay of human mammary carcinomas for estrogen receptors is of proven clinical utility, but the cellular localization of estrogen binding siteswithin these lesions is less certain. This thesis describes the identification of estrogen binding sites as visualized by thaw-mount autoradiography after in vitro incubation in a series of 17 benign and 40 malignant human female mammary lesions. The results of the in vitro incubation method compared favorably with data from in vivo studies in mouse uterus, a well-characterized estrogen target organ. In noncancerous breast biopsies a variable proportion of epithelial cells contained specific estrogen binding sites. Histologically identifiable myoepithelial and stromal cells were, in general, unlabeled. In human mammary carcinomas, biochemically estrogen receptor-positive, labeled and unlabeled neoplastic epithelial cells were identified by autoradiography. Quantitative results from the autoradiographic method compared favorably with biochemical data.

Advisors/Committee Members: Tremblay, Gilles.

Subjects/Keywords: Estrogen receptors

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APA (6^th Edition):

Buell, R. H. (1984). The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary Lesions. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/fb494b707.pdf ; https://escholarship.mcgill.ca/concern/theses/tb09j792p

Chicago Manual of Style (16^th Edition):

Buell, Richard H. “The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary Lesions.” 1984. Doctoral Dissertation, McGill University. Accessed January 25, 2021. https://escholarship.mcgill.ca/downloads/fb494b707.pdf ; https://escholarship.mcgill.ca/concern/theses/tb09j792p.

MLA Handbook (7^th Edition):

Buell, Richard H. “The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary Lesions.” 1984. Web. 25 Jan 2021.

Vancouver:

Buell RH. The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary Lesions. [Internet] [Doctoral dissertation]. McGill University; 1984. [cited 2021 Jan 25]. Available from: https://escholarship.mcgill.ca/downloads/fb494b707.pdf ; https://escholarship.mcgill.ca/concern/theses/tb09j792p.

Council of Science Editors:

Buell RH. The Autoradiographic Localization of Estrogen Binding Sites in Human Mammary Lesions. [Doctoral Dissertation]. McGill University; 1984. Available from: https://escholarship.mcgill.ca/downloads/fb494b707.pdf ; https://escholarship.mcgill.ca/concern/theses/tb09j792p

5. Sakka, Christina. Η επίδραση του πολυμορφισμού των οιστρογονικών υποδοχέων στα επίπεδα των στερεοειδών ορμονών και των λιπιδίων αίματος στους άνδρες.

Degree: 2015, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας

URL: http://hdl.handle.net/10442/hedi/36327

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ABSTRACTObjective: Estrogens play an important role in male physiology. We investigated the possible association of four single nucleotide polymorphisms in Estrogen Receptor α and Estrogen… (more)

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ABSTRACTObjective: Estrogens play an important role in male physiology. We investigated the possible association of four single nucleotide polymorphisms in Estrogen Receptor α and Estrogen Receptor β genes with circulating levels of sex steroids and lipid levels in men.Design and Methods: SHBG, total and calculated free testosterone (TT and cal FT), estradiol (E2) and free Estradiol (FE2 ) Lipids, glucose, insulin and HOMA-IR were determined in a population-based cohort of 170 apparently healthy Greek men. Body mass index (BMI), waist circumference (WC) and percentage of body fat (%fat) content were measured in all participants. Genotyping for the PvuII and XbaI polymorphisms of the ESR1 gene and for the RsaI and AluI polymorphisms of the ESR2 gene was performed.Results: PvuII showed an association with E2 levels [median (IQR) pp 58.5 (42.1-73.4) pg/ml vs. Pp 48.8 (42.9-60.1) and PP 57.7 (44-70.5), p=0.032], and with %fat [mean±SD pp 24.6±5.3 vs Pp 22.4±5.2 and PP 21.2±6.7, p=0.044], after adjustment for age and WC. Furthermore, the effect of PvuII on E2 was independent of %fat (p=0.038).A synergistic effect of the two ESR1 polymorphisms on E2 (p=0.023), FE2 (p=0.03) and %fat (p=0.004) was present. Finally, a synergistic effect of the ESR1 and ESR2 genes on TT (p=0.009), independent of age, WC and %fat also emerged.Associations of AluI with LDL-Chol (mean±SD, aa 4.3±1.1 vs. Aa 3.7±1.0 and ΑΑ 4.2±1.1, p=0.023) and RsaI with HOMA-IR [median(IQR) RR 1.55(0.88-2.49) vs. Rr/rr 1.69(0.72-2.29), p=0.032] were found. Synergistic effects of RsaI and AluI of ESR2 gene on LDL-Chol levels, %fat and WC, as well as a synergistic effect of both ESR1 and ESR2 genes on levels of T-Chol (p=0.01) and LDL-Chol (p=0.027) were also shown. These findings remained significant after adjustment for potential confounders.Significant independent associations of PvuII with %fat (mean±SD, pp 24.6±5.3 vs Pp 22.4±5.2 and PP 21.2±6.7, p=0.044), and RsaI with % fat (RR 22.6 ±5.5 vs. Rr/rr 25.2 ±6.3, p=0.015) and WC (mean±SD RR 97.4 ±10.4 vs. Rr/rr 102.6 ±12.6, p=0.013) were found.Synergistic effects on %fat, between the ESR1 polymorphisms (p=0.004), between the ESR2 polymorphisms and among all four ESR polymorphisms studied were also present.Conclusions: Genetic variation in ER1 is associated with serum estradiol levels and body fat content regulation in men. Furthermore, a synergistic effect of ESR1 and ESR2 genes is exerted on serum testosterone levels. ESR2 is associated with LDL-Chol levels in men independently of confounders. Furthermore, a synergistic effect of ESR1 and ESR2 on T-Chol, LDL-Chol and %fat, was shown.

ΠΕΡΙΛΗΨΗΣκοπός : Τα οιστρογόνα παίζουν σημαντικό ρόλο στην ανδρική φυσιολογία. Μελετήσαμε την πιθανή σχέση τεσσάρων SNPs( single nucleotide polymorphisms) του οιστρογονικού υποδοχέα α και β στα επίπεδα των στεροειδών του φύλου και των λιπιδίων στους άνδρες.Μέθοδος : Μετρήθηκε SHBG, η ολική και ελεύθερη τεστοστερόνη (TT ,FT), οιστραδιόλη (E2)και (FE2 ) τα λιπίδια, η γλυκόζη ,η ινσουλίνη και ο HOMA-IR αι σε ένα πληθυσμό 170 υγιών…

Subjects/Keywords: Οιστρογονικοί υποδοχείς; Estrogen receptors

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APA (6^th Edition):

Sakka, C. (2015). Η επίδραση του πολυμορφισμού των οιστρογονικών υποδοχέων στα επίπεδα των στερεοειδών ορμονών και των λιπιδίων αίματος στους άνδρες. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/36327

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sakka, Christina. “Η επίδραση του πολυμορφισμού των οιστρογονικών υποδοχέων στα επίπεδα των στερεοειδών ορμονών και των λιπιδίων αίματος στους άνδρες.” 2015. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed January 25, 2021. http://hdl.handle.net/10442/hedi/36327.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sakka, Christina. “Η επίδραση του πολυμορφισμού των οιστρογονικών υποδοχέων στα επίπεδα των στερεοειδών ορμονών και των λιπιδίων αίματος στους άνδρες.” 2015. Web. 25 Jan 2021.

Vancouver:

Sakka C. Η επίδραση του πολυμορφισμού των οιστρογονικών υποδοχέων στα επίπεδα των στερεοειδών ορμονών και των λιπιδίων αίματος στους άνδρες. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2015. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10442/hedi/36327.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sakka C. Η επίδραση του πολυμορφισμού των οιστρογονικών υποδοχέων στα επίπεδα των στερεοειδών ορμονών και των λιπιδίων αίματος στους άνδρες. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2015. Available from: http://hdl.handle.net/10442/hedi/36327

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

6. Ribay, Kathryn, 1984-. Hybrid modeling of estrogen receptor binding agents using advanced cheminformatics tools and massive public data.

Degree: MS, Chemistry, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49078/

► Estrogen receptor-α (ERα) is a critical target for drug design as well as a potential source of toxicity when activated unintentionally. Thus, evaluating potential ERα… (more)

▼ Estrogen receptor-α (ERα) is a critical target for drug design as well as a potential source of toxicity when activated unintentionally. Thus, evaluating potential ERα binding agents is critical in both drug discovery and chemical toxicity areas. Using computational tools, e.g. Quantitative Structure-Activity Relationship (QSAR) models, can predict potential ERα binding agents before chemical synthesis. The purpose of this project was to develop enhanced predictive models of ERα binding agents by utilizing advanced cheminformatics tools that can integrate publicly available bioassay data. The initial ERα binding agent data set, consisting of 446 binders and 8,307 non-binders, was obtained from the Tox21 Challenge project organized by the NIH Chemical Genomics Center (NCGC). After removing the duplicates and inorganic compounds, this data set was used to create a training set (259 binders and 259 non-binders). This training set was used to develop QSAR models using chemical descriptors. The resulting models were then used to predict the binding activity of 264 external compounds, which were available to us after the models were developed. The cross-validation results of training set [Correct Classification Rate (CCR)) = 0.72] were much higher than the external predictivity of the unknown compounds (CCR= 0.59). To improve the conventional QSAR models, all compounds in the training set were used to search PubChem and generate a profile of their biological responses across thousands of bioassays. The most important bioassays were prioritized to generate a similarity index that was used to calculate the biosimilarity score between each two compounds. The nearest neighbors for each compound within the set were then identified and its ERα binding potential was predicted by its nearest neighbors in the training set. The hybrid model performance (CCR=0.94 for cross validation; CCR=0.68 for external prediction) showed significant improvement over the original QSAR models, particularly for the activity cliffs that induce prediction errors in conventional QSAR models. The results of this study indicate that the response profile of chemicals from public data provides useful information for modeling and evaluation purposes. The public big data resources should be considered along with chemical structure information when predicting new compounds, such as unknown ERα binding agents. Advisors/Committee Members: Zhu, Hao (chair), Martin, Joseph (internal member), Roche, Alex (internal member).

Subjects/Keywords: QSAR (Biochemistry); Estrogen – Receptors

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APA (6^th Edition):

Ribay, Kathryn, 1. (2016). Hybrid modeling of estrogen receptor binding agents using advanced cheminformatics tools and massive public data. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49078/

Chicago Manual of Style (16^th Edition):

Ribay, Kathryn, 1984-. “Hybrid modeling of estrogen receptor binding agents using advanced cheminformatics tools and massive public data.” 2016. Masters Thesis, Rutgers University. Accessed January 25, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49078/.

MLA Handbook (7^th Edition):

Ribay, Kathryn, 1984-. “Hybrid modeling of estrogen receptor binding agents using advanced cheminformatics tools and massive public data.” 2016. Web. 25 Jan 2021.

Vancouver:

Ribay, Kathryn 1. Hybrid modeling of estrogen receptor binding agents using advanced cheminformatics tools and massive public data. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Jan 25]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49078/.

Council of Science Editors:

Ribay, Kathryn 1. Hybrid modeling of estrogen receptor binding agents using advanced cheminformatics tools and massive public data. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49078/

University of Houston

7. Butler, Ryan 1986-. Physiological and pathological aspects of the ligand-activated transcription factors: AhR and ERβ.

Degree: PhD, Biology, 2013, University of Houston

URL: http://hdl.handle.net/10657/956

► Ligand-activated transcription factors are a diverse group of proteins that are involved a variety of physiological processes. The purpose of these studies was to investigate… (more)

▼ Ligand-activated transcription factors are a diverse group of proteins that are involved a variety of physiological processes. The purpose of these studies was to investigate the aryl hydrocarbon receptor (AhR) knockout mouse and the effects of hormone replacement therapy on the postmenopausal breast in order to better understand the functions of the ligand-activated transcription factors AhR and estrogen receptor β (ERβ). In the first part of these studies, we investigated the AhR knockout mouse in order to further elucidate the physiological functions of AhR. These mice developed stones in their urinary bladders composed of 100% uric acid while the serum uric acid levels remained normal. We determined these stones were formed by the breakdown of DNA into uric acid from a large number of dying cells. These mice also develop fibrosis of their ventral prostates and a phenotype in the immune system similar to chronic myeloid leukemia. The second part of these studies was to investigate the effects of hormone replacement therapy on the histology and expression of various ligand-activated transcription factors in the postmenopausal breast. We treated postmenopausal women with estrogen or estrogen-progesterone therapy for 3 months and took biopsies of the breasts before and after treatment. Expression of several proteins including AhR, ERα, and ERβ were unchanged after treatment while expression of progesterone receptors was increased. Proliferation and breast density were unchanged by treatment in these breasts; however, we show possible mechanisms leading to proliferation and development of density in non-cancerous breast tissue. In conclusion, these studies revealed a role for AhR in prostatic development, uric acid formation, and leukemia, and have provided new information on the safety of short-term use of HRT. Advisors/Committee Members: Gustafsson, Jan-Åke (advisor), Warner, Margaret (committee member), Ziburkus, Jokubas (committee member), Webb, Paul (committee member).

Subjects/Keywords: Aryl hydrocarbon receptor; Estrogen receptors; Transcription factors

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APA (6^th Edition):

Butler, R. 1. (2013). Physiological and pathological aspects of the ligand-activated transcription factors: AhR and ERβ. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/956

Chicago Manual of Style (16^th Edition):

Butler, Ryan 1986-. “Physiological and pathological aspects of the ligand-activated transcription factors: AhR and ERβ.” 2013. Doctoral Dissertation, University of Houston. Accessed January 25, 2021. http://hdl.handle.net/10657/956.

MLA Handbook (7^th Edition):

Butler, Ryan 1986-. “Physiological and pathological aspects of the ligand-activated transcription factors: AhR and ERβ.” 2013. Web. 25 Jan 2021.

Vancouver:

Butler R1. Physiological and pathological aspects of the ligand-activated transcription factors: AhR and ERβ. [Internet] [Doctoral dissertation]. University of Houston; 2013. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10657/956.

Council of Science Editors:

Butler R1. Physiological and pathological aspects of the ligand-activated transcription factors: AhR and ERβ. [Doctoral Dissertation]. University of Houston; 2013. Available from: http://hdl.handle.net/10657/956

Uppsala University

8. Chen, Gunilla. Changes in adipose tissue mRNA expression due to perinatal exposure to bisphenol A in rats.

Degree: Women's and Children's Health, 2014, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235115

► Bisphenol A (BPA) is an estrogen receptor binding chemical, widely used in the plastics industry, and as such commonly encountered from plastic containers etc.… (more)

Subjects/Keywords: Obesity; endocrine disruptor; metabolism; PPARγ; estrogen receptors

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APA (6^th Edition):

Chen, G. (2014). Changes in adipose tissue mRNA expression due to perinatal exposure to bisphenol A in rats. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235115

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Gunilla. “Changes in adipose tissue mRNA expression due to perinatal exposure to bisphenol A in rats.” 2014. Thesis, Uppsala University. Accessed January 25, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235115.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Gunilla. “Changes in adipose tissue mRNA expression due to perinatal exposure to bisphenol A in rats.” 2014. Web. 25 Jan 2021.

Vancouver:

Chen G. Changes in adipose tissue mRNA expression due to perinatal exposure to bisphenol A in rats. [Internet] [Thesis]. Uppsala University; 2014. [cited 2021 Jan 25]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235115.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen G. Changes in adipose tissue mRNA expression due to perinatal exposure to bisphenol A in rats. [Thesis]. Uppsala University; 2014. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235115

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Anna University

9. Sabarinath, C. Estrogenic effects of in silico designed isoflavone and deoxybenzoin derivatives on human cancer cells; -.

Degree: Technology, 2014, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/24268

►

Estrogens are key regulators of human reproductive physiology acting through estrogen receptors In humans there are two major estrogen receptor subtypes and they are estrogen… (more)

Subjects/Keywords: Deoxybenzoin; Estrogen dependent cell; Estrogen receptors; Isoflavones; Technology

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APA (6^th Edition):

Sabarinath, C. (2014). Estrogenic effects of in silico designed isoflavone and deoxybenzoin derivatives on human cancer cells; -. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/24268

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sabarinath, C. “Estrogenic effects of in silico designed isoflavone and deoxybenzoin derivatives on human cancer cells; -.” 2014. Thesis, Anna University. Accessed January 25, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/24268.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sabarinath, C. “Estrogenic effects of in silico designed isoflavone and deoxybenzoin derivatives on human cancer cells; -.” 2014. Web. 25 Jan 2021.

Vancouver:

Sabarinath C. Estrogenic effects of in silico designed isoflavone and deoxybenzoin derivatives on human cancer cells; -. [Internet] [Thesis]. Anna University; 2014. [cited 2021 Jan 25]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/24268.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sabarinath C. Estrogenic effects of in silico designed isoflavone and deoxybenzoin derivatives on human cancer cells; -. [Thesis]. Anna University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/24268

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Malmö University

10. Nebel, Daniel. Functional importance of estrogen receptors in the periodontium .

Degree: Malmö University. Faculty of Odontology, 2012, Malmö University

URL: http://hdl.handle.net/2043/13331

► The main functions of estrogen are associated with reproduction. However, estrogen has been shown to be of functional importance also in non-classic target organs. Previous… (more)

▼ The main functions of estrogen are associated with reproduction. However, estrogen has been shown to be of functional importance also in non-classic target organs. Previous studies, especially epidemiologic and clinical ones, have addressed estrogen’s influence on periodontitis, suggesting that estrogen has a beneficial effect, but the biological mechanisms have not been identified. Estrogen exerts genomic effects in the target cells by binding to the nuclear receptors, estrogen receptor (ERs), ERα and ERβ. The expression of the two subtypes of ERs varies depending on the tissue. The overall objectives of this thesis were to study the functional importance of estrogen receptors in the periodontium with special focus on inflammation, and stimulators of inflammation and their signaling pathways. The thesis is based on the following five papers. In Paper I, effects of estrogen on E. coli LPS-induced PDL cell production of interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1) and C-reactive protein (CRP) are assessed, by using ELISA. Furthermore, effects of LPS and estrogen on the normal characteristics of the PDL cell such as collagen synthesis and cell proliferation is determined by using L-[3H]proline incorporation and measurement of DNA synthesis, respectively. Key findings: E.coli LPS stimulates PDL cell IL-6 and MCP-1 production but has no effect on the normal physiological properties of PDL cells. LPSinduced IL-6 and MCP-1 is not reversed by estrogen suggesting that estrogen has no anti-inflammatory effect in these experiments. In Paper II, we investigate the effects of ovariectomy and aging on tooth attachment in female mice by using morphometric analysis. Key findings: Withdrawal of female sex hormone production by ovariectomy has no effect on alveolar bone height and apical termination of the junctional epithelium. In a second series of experiments these parameters are similar in mice sacrificed at 8-26 weeks of age, suggesting that tooth attachment is preserved with age in mice within a period of six months. In Paper III, the objective is to investigate the regulation of CCL2/MCP-1, CCL3/MIP-1α, and CCL5/RANTES chemokines by estrogen in human PDL cells by determining mRNA transcript levels (using quantitative real-time PCR) and protein levels (using ELISA). Key findings: A physiological concentration of estrogen reduces the expression of CCL3 mRNA by about 40% compared to PDL cells treated with LPS alone. In contrast, inter-individual differences in the effects of estrogen on CCL5 mRNA expression are observed. These findings indicate that estrogen affects chemokine expression in PDL cells showing a complex pattern involving down-regulation as well as up-regulation of chemokines. Estrogen exerts both anti-inflammatory and pro-inflammatory effects through these mechanisms. In Paper IV, ER expression in human gingival biopsies, and effects of estrogen on cultured gingival epithelial cell (HGEP) proliferation, are investigated. Expression of ERα and ERβ is determined by…

Subjects/Keywords: Estrogen; Estrogen receptors; LPS; Periodontium; PDL cells; Gingival epithelial cells

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APA (6^th Edition):

Nebel, D. (2012). Functional importance of estrogen receptors in the periodontium . (Thesis). Malmö University. Retrieved from http://hdl.handle.net/2043/13331

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nebel, Daniel. “Functional importance of estrogen receptors in the periodontium .” 2012. Thesis, Malmö University. Accessed January 25, 2021. http://hdl.handle.net/2043/13331.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nebel, Daniel. “Functional importance of estrogen receptors in the periodontium .” 2012. Web. 25 Jan 2021.

Vancouver:

Nebel D. Functional importance of estrogen receptors in the periodontium . [Internet] [Thesis]. Malmö University; 2012. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/2043/13331.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nebel D. Functional importance of estrogen receptors in the periodontium . [Thesis]. Malmö University; 2012. Available from: http://hdl.handle.net/2043/13331

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

11. Lipovka, Yulia. Estrogen Dependent Regulation of the Amp-Activated Protein Kinase Pathway .

Degree: 2015, University of Arizona

URL: http://hdl.handle.net/10150/556852

► Sex differences exist in the progression of heart disease, as premenopausal women are protected from developing severe hypertension, aortic stenosis, myocardial infarction and hypertrophic cardiomyopathies.… (more)

▼ Sex differences exist in the progression of heart disease, as premenopausal women are protected from developing severe hypertension, aortic stenosis, myocardial infarction and hypertrophic cardiomyopathies. The susceptibility and progression of cardiovascular disease increases in post-menopausal women. This is at least partially underlined by a pronounced decrease in circulating estrogen levels. Estradiol (E2), the most abundant estrogen in premenopausal women, is known to be cardioprotective. Recently, AMP-activated protein kinase (AMPK) has emerged as a prominent player in the development of cardiac hypertrophy and heart failure. AMPK is central to the energetic metabolism of the cell and is activated in response to energy deprivation. E2 has been shown to activate AMPK, by yet an unknown mechanism. The first part of this dissertation focuses on describing the molecular mechanism behind this AMPK activation. We found that E2 activates AMPK through a non- genomic pathway and involves direct interaction of classical estrogen receptors (ERα and ERβ) with the α-catalytic subunit of AMPK. These receptors also associate with the upstream kinase LKB1, which is required for E2-dependent activation of AMPK. Furthermore, the two estrogen receptors play opposite roles, where ERα increases AMPK activation, and ERβ acts as a repressor, inhibiting AMPK phosphorylation. To translate our findings to heart disease, the next step was to determine the effect of ovarian failure, underlined by E2 loss, on AMPK signaling during the progression of cardiac hypertrophy. We hypothesized that ovarian failure decreases cardiac AMPK signaling, translating in worsening of hypertrophy. We found that the status of cardiac AMPK signaling depends on the nature of the hypertrophic stimulus and the timing of ovarian failure in relation to the onset of hypertrophy. Furthermore, we did not detect any differences in the development of cardiac hypertrophy between wild type mice and mice in ovarian failure, which most likely occur down the line. In summary we described a novel mechanism of AMPK activation by the hormone E2. We also explored the effect of estrogen loss on cardiac AMPK activity, and found that it is dependent on factors such as the pathological state of the heart and timing of the intervention. These findings add to our understanding of the molecular mechanisms behind sex differences in energy handling and in the future could be translated into better therapeutics for the treatment of cardiac pathologies. Advisors/Committee Members: Konhilas, John P (advisor), Brooks, Heddwen (committeemember), Elfring, Lisa (committeemember), Doetschman, Thomas (committeemember), Tsao, Tsu-Shuen (committeemember), Konhilas, John P. (committeemember).

Subjects/Keywords: Breast cancer; Estradiol; Estrogen; Estrogen receptors; VCD; Molecular & Cellular Biology; AMPK

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lipovka, Y. (2015). Estrogen Dependent Regulation of the Amp-Activated Protein Kinase Pathway . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/556852

Chicago Manual of Style (16^th Edition):

Lipovka, Yulia. “Estrogen Dependent Regulation of the Amp-Activated Protein Kinase Pathway .” 2015. Doctoral Dissertation, University of Arizona. Accessed January 25, 2021. http://hdl.handle.net/10150/556852.

MLA Handbook (7^th Edition):

Lipovka, Yulia. “Estrogen Dependent Regulation of the Amp-Activated Protein Kinase Pathway .” 2015. Web. 25 Jan 2021.

Vancouver:

Lipovka Y. Estrogen Dependent Regulation of the Amp-Activated Protein Kinase Pathway . [Internet] [Doctoral dissertation]. University of Arizona; 2015. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10150/556852.

Council of Science Editors:

Lipovka Y. Estrogen Dependent Regulation of the Amp-Activated Protein Kinase Pathway . [Doctoral Dissertation]. University of Arizona; 2015. Available from: http://hdl.handle.net/10150/556852

University of Arizona

12. Hild-Petito, Sheri Ann. Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum.

Degree: 1988, University of Arizona

URL: http://hdl.handle.net/10150/184485

► Both estradiol and progeterone are proposed autocrine or paracrine regulators of ovarian function in primate species. However, specific receptors for these steroids have not been… (more)

▼ Both estradiol and progeterone are proposed autocrine or paracrine regulators of ovarian function in primate species. However, specific receptors for these steroids have not been localized to individual compartments of the primate ovary. Using immunocytochemical techniques, estradiol receptors were detected in the germinal epithelium, but not other structures, of ovaries obtained from rhesus or cynomolgus monkeys during the follicular and luteal phases of the menstrual cycle. In contrast, progesterone receptors were present in stromal and interstitial tissue, the thecal layers of healthy and atretic follicles, as well as the functional corpus luteum. These results are consistent with the concept of a receptor-mediated role for progesterone, but not estrogen, within the predominant gametogenic and endocrine structures, e.g., the follicle and corpus luteum, of the primate ovary. The recent discovery of distinct cell types in the corpus luteum of domestic ungulates has revised concepts on the control of luteal function in these species. Studies were designed to test the hypothesis that the primate corpus luteum consists of cell subpopulations that differ in physical characteristics, function and regulation. Cells enzymatically-dispersed from the monkey corpus luteum at mid-luteal phase of the menstrual cycle differed in size (diameter) and the presence of the steroidogenic enzyme, 3β-hydroxysteroid dehydrogenase (3β-HSD). Analysis of dispersed cells for forward and 90° light scatter properties by flow cytometry revealed two distinct continua (Cα and Cβ). These continua were isolated using the sorting capabilities of the flow cytometer. Cα contained single cells of ≤ 15 μm and cell clusters; the cells were typically 3β-HSD-negative nonsteroidogenic. Cβ consisted of single cells that increased in size up to 40 μm and were 3β-HSD-positive. Cβ was divided into two regions (R₁ and R₃) and the cells isolated. R₁ cells were ≤ 15 μm whereas R₃ cells were ≥ 20 μm. Basal progesterone and estrogen production by R₃ cells was greater than that produced by R₁ cells (as determined by radioimmunoassay of the incubation media). Relative stimulation of progesterone production by hCG, cAMP or PGE₂ was not different between R₁ and R₃ luteal cells. These results support the hypothesis that the primate corpus luteum consists of distinct cell subpopulations which differ in size and steroidogenic capacity. However, the cell types which secrete progesterone are typically responsive to gonadotropin and PGE₂, possibly via a cAMP-mediated pathway.

Subjects/Keywords: Estrogen – Receptors.; Progesterone – Receptors.; Hormone receptors.

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APA (6^th Edition):

Hild-Petito, S. A. (1988). Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/184485

Chicago Manual of Style (16^th Edition):

Hild-Petito, Sheri Ann. “Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum. ” 1988. Doctoral Dissertation, University of Arizona. Accessed January 25, 2021. http://hdl.handle.net/10150/184485.

MLA Handbook (7^th Edition):

Hild-Petito, Sheri Ann. “Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum. ” 1988. Web. 25 Jan 2021.

Vancouver:

Hild-Petito SA. Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum. [Internet] [Doctoral dissertation]. University of Arizona; 1988. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10150/184485.

Council of Science Editors:

Hild-Petito SA. Distribution of estrogen and progesterone receptors in the primate ovary, with emphasis on subpopulations of cells within the corpus luteum. [Doctoral Dissertation]. University of Arizona; 1988. Available from: http://hdl.handle.net/10150/184485

Dalhousie University

13. Wibowo, Erik. MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY.

Degree: PhD, Department of Medical Neuroscience, 2013, Dalhousie University

URL: http://hdl.handle.net/10222/36281

► Advanced prostate cancer (PCa) patients are offered androgen deprivation therapy (ADT) to control their cancer’s growth. ADT impairs sexual function and the sleep patterns of… (more)

▼ Advanced prostate cancer (PCa) patients are offered androgen deprivation therapy (ADT) to control their cancer’s growth. ADT impairs sexual function and the sleep patterns of ADT patients. Since ADT deprives patients of estrogen, and supplemental estrogen reduces such problems in menopausal women, I studied whether administering estrogen reduces these problems for castrated male rats as a model for PCa patients on ADT. First, I tested how early versus late estradiol treatment after castration influenced rats’ sexual behaviour. Estradiol increases mounting behaviour to comparable levels regardless of when the treatment was started after castration, suggesting that estrogen’s ability to restore male sexual interest is insensitive to a delay since castration. Secondly, to understand the biological basis of these behavioural effects, I examined brain and muscle tissues from the same animals. Specifically, I compared changes in 1) estrogen receptors (ERs) and c-Fos protein (a neuronal activation marker) levels in brain areas controlling sex behavior; 2) ERs levels in pelvic floor muscles, important for erection; and 3) ERs levels in the hippocampus and prefrontal cortex. Prolonged castration increases ER? levels in the preoptic area (POA), a key brain area that regulates mating behaviour, and estradiol treatment reduced these effects. In the POA, mating-induced c-Fos expression was not affected by estradiol regardless of when the treatment began post-castration. Estrogen may upregulate ERs in pelvic floor muscles, and downregulate ERs in the hippocampus and prefrontal cortex, depending on administration time after castration. These findings suggest that mating activates POA neurons, and this activation induces mounting only in the presence of estrogen. Additionally, the duration after castration influences ER autoregulation in the pelvic floor muscles, hippocampus, and prefrontal cortex in response to estradiol. Lastly, I studied how estrogen modulates the sleep-wake behaviour of orchiectomized rats. Estradiol promotes baseline wakefulness during the dark period and prevents castration-induced impairment in sleep recovery after sleep deprivation. These findings suggest that estradiol may positively influence the sleep-wake behaviour of castrated males. Collectively, I demonstrate that estrogen administered to castrated rats improves sexual and sleep functions. It may similarly improve the quality of life of PCa patients on ADT. Advisors/Committee Members: Dr. Jessica Mong (external-examiner), Dr. Kazue Semba (graduate-coordinator), Dr. Tara Perrot, Dr. Richard Brown (thesis-reader), Dr. Kazue Semba, Dr. Richard Wassersug (thesis-supervisor), Received (ethics-approval), Yes (manuscripts), Yes (copyright-release).

Subjects/Keywords: Prostate Cancer; Androgen Deprivation Therapy; Estrogen; Sexual Interest; Sleep; Estrogen Receptors; Pelvic Floor Muscles

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APA (6^th Edition):

Wibowo, E. (2013). MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY. (Doctoral Dissertation). Dalhousie University. Retrieved from http://hdl.handle.net/10222/36281

Chicago Manual of Style (16^th Edition):

Wibowo, Erik. “MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY.” 2013. Doctoral Dissertation, Dalhousie University. Accessed January 25, 2021. http://hdl.handle.net/10222/36281.

MLA Handbook (7^th Edition):

Wibowo, Erik. “MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY.” 2013. Web. 25 Jan 2021.

Vancouver:

Wibowo E. MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY. [Internet] [Doctoral dissertation]. Dalhousie University; 2013. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10222/36281.

Council of Science Editors:

Wibowo E. MODULATION OF SEXUAL AND SLEEP FUNCTIONS BY ESTROGEN IN CASTRATED MALE RATS AS A MODEL FOR PROSTATE CANCER PATIENTS ON ANDROGEN DEPRIVATION THERAPY. [Doctoral Dissertation]. Dalhousie University; 2013. Available from: http://hdl.handle.net/10222/36281

University of the Western Cape

14. Jijana, Abongile Nwabisa. Quantum dot sensitized estrogen receptor alpha-recombinant protein electrochemical biosensor for 17-beta estradiol .

Degree: 2016, University of the Western Cape

URL: http://hdl.handle.net/11394/5330

► Estrogens play an extraordinary role in the endocrine system regulation through the stimulation and regulation of endocrine pathways. 17β-estradiol is one of the final metabolites… (more)

▼ Estrogens play an extraordinary role in the endocrine system regulation through the stimulation and regulation of endocrine pathways. 17β-estradiol is one of the final metabolites in estrogen regulation by hydroxylase enzymes that are well recognized for their metabolic role in hormone fragmentation and dissociation, through hydroxylation reactions that reversibly convert a series of androgens to estrogens (i.e. or one estrogen to the other). However, the 17β-estradiol hormone has been classified as one of the estrogenic endocrine disrupting compounds {i.e. EDC (s)} that show significant adverse effects in the estrogen pathways of male and female animal species. Estrogen receptor alpha (ER-α) is significantly activated by 17β-estradiol, which is a steroid hormone. A biosensor system for the determination of 17β-estradiol was developed based on the highly selective and specific physiological substrate level activation of the ER-α biomolecule by the (17β-estradiol) compound. The chemically-tuned tin selenide quantum dots capped with 3-mercaptopropionic acid were produced at room temperature and employed to capture the ER-α micro-molecule onto the electrode surfaces. These quantum dots possessed average particle size (APS) diameters between 4.6 ± 0.6 nm and an indirect band gap energy (Eg) of 3.14 eV. Surface modification on the quantum dots permitted the formation of efficient amide bonds between the capping molecules of the quantum dots and the estrogen receptor-alpha. The tin selenide quantum dots platform enhanced the surface bio-reactivity of the receptorsensor film. The receptorsensor’s sensitivity towards 17β-estradiol was 5.9 μA/μM associated with a response time (tResponse) of less than 1.2 s. The formal potential, Ep˚ˈ, of the receptorsensor-substrate complex was 149 mV. A detection limit (DL) of 1.9 nM was obtained for the electrochemical biosensing methodology. 17β-estradiol–receptorsensor response kinetics were also evaluated, where a dissociation rate (kd) of 7.6 μM/s, a 50 % inhibition concentration (IC50) value of 3.4 nM and a binding efficiency (Bmax) of 7 nM were obtained. Effective measure of 17β-estradiol concentrations as low as 3.8 nM present in surface waters have been reported to induce feminisation in male aquatic species. The receptorsensor’s dynamic linear range (DLR) nevertheless showed capability of screening a minimum of 0.2 nM to a maximum of 8 nM of the 17β-estradiol concentrations. Furthermore, during the estrogen replacement therapy (ERT), 17β-estradiol concentration levels are monitored at frequent phases, wherein 17β-estradiol concentrations from as low as 0.37 nM are recovered in the serum (i.e. this value was also evaluated to be within the receptorsensor’s-DLR), determining its future capability to be developed for; clinical-diagnosis screening of the 17β-estradiol. Advisors/Committee Members: Iwuoha, Emmanuel (advisor), Baker, Priscilla G.L (advisor).

Subjects/Keywords: Amino acids; Proteins; Fluorescence spectroscopy; Estrogen – Receptors; Estrogen receptor-alpha (ER-𝜶�)

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APA (6^th Edition):

Jijana, A. N. (2016). Quantum dot sensitized estrogen receptor alpha-recombinant protein electrochemical biosensor for 17-beta estradiol . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/5330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jijana, Abongile Nwabisa. “Quantum dot sensitized estrogen receptor alpha-recombinant protein electrochemical biosensor for 17-beta estradiol .” 2016. Thesis, University of the Western Cape. Accessed January 25, 2021. http://hdl.handle.net/11394/5330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jijana, Abongile Nwabisa. “Quantum dot sensitized estrogen receptor alpha-recombinant protein electrochemical biosensor for 17-beta estradiol .” 2016. Web. 25 Jan 2021.

Vancouver:

Jijana AN. Quantum dot sensitized estrogen receptor alpha-recombinant protein electrochemical biosensor for 17-beta estradiol . [Internet] [Thesis]. University of the Western Cape; 2016. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/11394/5330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jijana AN. Quantum dot sensitized estrogen receptor alpha-recombinant protein electrochemical biosensor for 17-beta estradiol . [Thesis]. University of the Western Cape; 2016. Available from: http://hdl.handle.net/11394/5330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Loma Linda University

15. Richardson, Angelique. IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells.

Degree: PhD, Basic Sciences, 2011, Loma Linda University

URL: https://scholarsrepository.llu.edu/etd/60

► The autocrine-paracrine effects of IGF2 are important in the growth and differentiation of normal breast. In breast cancer (BC), IGF2 is initially stimulated by estrogen,… (more)

Subjects/Keywords: Medical Genetics; Medical Microbiology; Estrogen; Breast Cancer;

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APA (6^th Edition):

Richardson, A. (2011). IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells. (Doctoral Dissertation). Loma Linda University. Retrieved from https://scholarsrepository.llu.edu/etd/60

Chicago Manual of Style (16^th Edition):

Richardson, Angelique. “IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells.” 2011. Doctoral Dissertation, Loma Linda University. Accessed January 25, 2021. https://scholarsrepository.llu.edu/etd/60.

MLA Handbook (7^th Edition):

Richardson, Angelique. “IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells.” 2011. Web. 25 Jan 2021.

Vancouver:

Richardson A. IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells. [Internet] [Doctoral dissertation]. Loma Linda University; 2011. [cited 2021 Jan 25]. Available from: https://scholarsrepository.llu.edu/etd/60.

Council of Science Editors:

Richardson A. IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells. [Doctoral Dissertation]. Loma Linda University; 2011. Available from: https://scholarsrepository.llu.edu/etd/60

University of Toledo Health Science Campus

16. Jetson, Rachael Rene. Design and Development of Potential Therapeutic Agents for Use in Hormone Responsive Cancers.

Degree: PhD, College of Pharmacy, 2013, University of Toledo Health Science Campus

URL: http://rave.ohiolink.edu/etdc/view?acc_num=mco1384270219

► This thesis describes medicinal chemistry methods aimed towards the understanding of estrogen receptors (ERs) and breast cancer. Breast cancer is the second most common cancer… (more)

▼ This thesis describes medicinal chemistry methods aimed towards the understanding of estrogen receptors (ERs) and breast cancer. Breast cancer is the second most common cancer and the second leading cause of cancer fatalities in women in the United States. These devastating statistics are partially attributed to unreliable diagnosis, reoccurrence and drug resistance. Approximately 70% of these cancers express ERs, making them a relevant target for the development of new therapeutics and investigation into the mechanism of this disorder.A major portion of this project focuses on the design and synthesis of compound libraries directed towards ERs that will utilize natural products as their starting point. From a family of compounds called the flavonoids that contain estrogenic (glycinol) and anti-estrogenic (glyceollins) members, we devised new structures that are meant to exploit key binding interactions within the ER. Studies leading to these target compound libraries include the design and synthesis of `model’ compound libraries meant to mimic the target structure’s corresponding pharmacophores. By developing the model libraries we were able to explore synthetic methods that could be used for the synthesis of the targets. Additionally, one set of these libraries was also used to obtain initial biological data to develop structure-activity relationships that, in turn, contributed to final target selection.To further support our target design we studied our compound libraries (target and model) via ER docking paradigms derived from protein database starting points. To properly conduct these experiments we first determined an appropriate protein docking model for our natural product system. This docking model was designed in such a way as to best describe the biological profile of known agents and a group of our natural products. Using this protein docking model, we examined our compound libraries allowing for a more direct comparison between the two systems.Additional studies were directed toward the androgen receptor (AR) and prostate cancer. The rationale for these studies includes implications that androgens and ARs are involved in breast cancer. Furthermore, the glyceollin group from the flavonoid family has shown anti-androgen potential. Similar to the ER experiments, an X-ray derived protein docking model for the AR was devised to describe the biological profile of known agents and some of the glyceollins. This docking model was then used to study all the compound libraries.A final project was an investigation into the role of retinoic acid receptors (RARs) in breast cancer. Preliminary research implies that RARs may play a role in breast cancer drug resistance. In order to help address this hypothesis, we first synthesized a known agent to probe the RAR subtype involved in breast cancer, RARa. Secondly, we suggested other possible agents that may work as scaffolds for designing new, more specific probes. We devised a new synthetic route to these agents that ultimately led to higher yields, lower cost and… Advisors/Committee Members: Erhardt, Paul (Advisor).

Subjects/Keywords: Chemistry; Organic Chemistry; Pharmacy Sciences; Biology; Breast cancer; Glyceollins; Estrogen Receptors; Retinoic Acid Receptors; Selective Estrogen Receptor Modulators

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APA (6^th Edition):

Jetson, R. R. (2013). Design and Development of Potential Therapeutic Agents for Use in Hormone Responsive Cancers. (Doctoral Dissertation). University of Toledo Health Science Campus. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=mco1384270219

Chicago Manual of Style (16^th Edition):

Jetson, Rachael Rene. “Design and Development of Potential Therapeutic Agents for Use in Hormone Responsive Cancers.” 2013. Doctoral Dissertation, University of Toledo Health Science Campus. Accessed January 25, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=mco1384270219.

MLA Handbook (7^th Edition):

Jetson, Rachael Rene. “Design and Development of Potential Therapeutic Agents for Use in Hormone Responsive Cancers.” 2013. Web. 25 Jan 2021.

Vancouver:

Jetson RR. Design and Development of Potential Therapeutic Agents for Use in Hormone Responsive Cancers. [Internet] [Doctoral dissertation]. University of Toledo Health Science Campus; 2013. [cited 2021 Jan 25]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1384270219.

Council of Science Editors:

Jetson RR. Design and Development of Potential Therapeutic Agents for Use in Hormone Responsive Cancers. [Doctoral Dissertation]. University of Toledo Health Science Campus; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1384270219

17. Williams, Derrick Wayne. Effects of co-activation of cannabinoid type 2 and estrogen receptors on osteogenesis in cultured primary human osteoblasts.

Degree: MS(M.S.), Biology, 2018, Colorado State University – Pueblo

URL: http://hdl.handle.net/10217/193048

► Postmenopausal osteoporosis develops when bone removal by osteoclasts exceeds bone formation by osteoblasts due, in part, to the loss of the bone protective effects of… (more)

Subjects/Keywords: Endocannabinoid receptors; CB2 receptors; Cannabinoid type 2 receptors; Primary human osteoblasts; AM1241; AM630; 17b-estradiol; Osteonenesis; Osteoblasts; Cannabinoids; Bones – Growth; Estrogen – Receptors; Estrogen – Agonists

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Williams, D. W. (2018). Effects of co-activation of cannabinoid type 2 and estrogen receptors on osteogenesis in cultured primary human osteoblasts. (Masters Thesis). Colorado State University – Pueblo. Retrieved from http://hdl.handle.net/10217/193048

Chicago Manual of Style (16^th Edition):

Williams, Derrick Wayne. “Effects of co-activation of cannabinoid type 2 and estrogen receptors on osteogenesis in cultured primary human osteoblasts.” 2018. Masters Thesis, Colorado State University – Pueblo. Accessed January 25, 2021. http://hdl.handle.net/10217/193048.

MLA Handbook (7^th Edition):

Williams, Derrick Wayne. “Effects of co-activation of cannabinoid type 2 and estrogen receptors on osteogenesis in cultured primary human osteoblasts.” 2018. Web. 25 Jan 2021.

Vancouver:

Williams DW. Effects of co-activation of cannabinoid type 2 and estrogen receptors on osteogenesis in cultured primary human osteoblasts. [Internet] [Masters thesis]. Colorado State University – Pueblo; 2018. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10217/193048.

Council of Science Editors:

Williams DW. Effects of co-activation of cannabinoid type 2 and estrogen receptors on osteogenesis in cultured primary human osteoblasts. [Masters Thesis]. Colorado State University – Pueblo; 2018. Available from: http://hdl.handle.net/10217/193048

Columbia University

18. Work, Meghan E. Breast Cancer Risk Factors and Associations with Breast Cancer Tumor Characteristics in High Risk Populations.

Degree: 2018, Columbia University

URL: https://doi.org/10.7916/D8903MBW

► Background: Estrogen receptor (ER)- and progesterone receptor (PR)-negative (ER-PR-) breast cancer is associated with higher grade and poorer prognosis compared with other breast cancer subtypes.… (more)

▼ Background: Estrogen receptor (ER)- and progesterone receptor (PR)-negative (ER-PR-) breast cancer is associated with higher grade and poorer prognosis compared with other breast cancer subtypes. High parity, coupled with lack of breastfeeding, has been associated with an increased risk of ER-PR- cancer. The mechanism of this etiology is unclear, and may be obfuscated by ER and PR correlation with each other as well as other prognostic tumor characteristics. Methods: Using population-based and clinic-based ascertained cases and controls from the Breast Cancer Family Registry, I examined reproductive risk factors, including parity, breastfeeding, and oral contraceptive (OC) use, in relation to ER and PR status, using polytomous logistic regression (for the population-based data) and the method of generalized estimating equations (GEE) (for the clinic-based data) as well as the pseudo-conditional likelihood approach, which accounts for correlated outcome variables. Results: High parity (≥ 3 live births) combined with lack of breastfeeding, was positively associated with ER-PR- tumors (odds ratio [OR]=1.57, 95% confidence interval [CI] 1.10-2.24, population-based cases vs. controls) relative to nulliparity. There was no association with ER-PR- tumors and parity in women who breastfed (OR=0.93, 95%CI 0.71-1.22) relative to nulliparous women. Associations with ER-PR- cancer were higher across all races/ethnicities among women who did not breastfeed compared with women who did. Population-based and clinic-based data were generally in agreement (OR=2.07, 95% CI 1.09-3.91, clinic-based cases vs. controls, relative to nulliparity). When adjusted for the correlation of PR-status and grade, to ER-status, the association between high parity +lack of breastfeeding and ER- status, was maintained. OC use before year 1975 was associated with an increased risk of ER-PR- tumors (OR=1.32, 95% CI 1.04-1.67, population-based data, cases vs. controls) relative to never use of OCs. For women who began OC use in 1975 or later there was no increased risk. Analysis of OC use in clinic-based data agreed with the findings of the population-based data. Conclusions: My findings support that there are modifiable factors for ER-PR- breast cancer, and that breastfeeding in particular may mitigate the increased risk of ER-PR-cancers seen from multiparity. The mechanism of both risk and risk mitigation may operate primarily through the estrogen, rather than progesterone, pathway.

Subjects/Keywords: Breastfeeding; Breast – Cancer – Epidemiology; Breast – Cancer – Risk factors; Breast – Tumors; Estrogen – Receptors; Progesterone – Receptors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Work, M. E. (2018). Breast Cancer Risk Factors and Associations with Breast Cancer Tumor Characteristics in High Risk Populations. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8903MBW

Chicago Manual of Style (16^th Edition):

Work, Meghan E. “Breast Cancer Risk Factors and Associations with Breast Cancer Tumor Characteristics in High Risk Populations.” 2018. Doctoral Dissertation, Columbia University. Accessed January 25, 2021. https://doi.org/10.7916/D8903MBW.

MLA Handbook (7^th Edition):

Work, Meghan E. “Breast Cancer Risk Factors and Associations with Breast Cancer Tumor Characteristics in High Risk Populations.” 2018. Web. 25 Jan 2021.

Vancouver:

Work ME. Breast Cancer Risk Factors and Associations with Breast Cancer Tumor Characteristics in High Risk Populations. [Internet] [Doctoral dissertation]. Columbia University; 2018. [cited 2021 Jan 25]. Available from: https://doi.org/10.7916/D8903MBW.

Council of Science Editors:

Work ME. Breast Cancer Risk Factors and Associations with Breast Cancer Tumor Characteristics in High Risk Populations. [Doctoral Dissertation]. Columbia University; 2018. Available from: https://doi.org/10.7916/D8903MBW

19. Bemd, Gert-Jan. Studies on the mechanism of action of synthetic ligands of the vitamin D and estrogen receptor.

Degree: Department of Plastic and Reconstructive Surgery, 2000, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/20903

► textabstractNuclear hormones play a key role in embtyonic development, growth and differentiation of cells and tissues, and in maintenance of homeostasis. They exert their action… (more)

Subjects/Keywords: enzymes; estrogen receptors; receptors; vitamine D

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bemd, G. (2000). Studies on the mechanism of action of synthetic ligands of the vitamin D and estrogen receptor. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/20903

Chicago Manual of Style (16^th Edition):

Bemd, Gert-Jan. “Studies on the mechanism of action of synthetic ligands of the vitamin D and estrogen receptor.” 2000. Doctoral Dissertation, Erasmus University Medical Center. Accessed January 25, 2021. http://hdl.handle.net/1765/20903.

MLA Handbook (7^th Edition):

Bemd, Gert-Jan. “Studies on the mechanism of action of synthetic ligands of the vitamin D and estrogen receptor.” 2000. Web. 25 Jan 2021.

Vancouver:

Bemd G. Studies on the mechanism of action of synthetic ligands of the vitamin D and estrogen receptor. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 2000. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/1765/20903.

Council of Science Editors:

Bemd G. Studies on the mechanism of action of synthetic ligands of the vitamin D and estrogen receptor. [Doctoral Dissertation]. Erasmus University Medical Center; 2000. Available from: http://hdl.handle.net/1765/20903

20. Menad, Rafik. Régulation fonctionnelle de l’épididyme d’un rongeur déserticole, Psammomys obesus, CRETZSCHMAR, 1828 : Functional Regulation of Epididymis of Sand Rat Psammomys obesus, CRETZSCHMAR, 1828.

Degree: Docteur es, Sciences biologiques, 2015, Paris, EPHE; Université des sciences et de la technologie Houari Boumediene (Alger)

URL: http://www.theses.fr/2015EPHE3011

►

Afin de mettre en évidence les principaux éléments de la voie androgénique et œstrogénique dans l’épididyme du rat des sables adulte, capturé dans la région… (more)

▼

Afin de mettre en évidence les principaux éléments de la voie androgénique et œstrogénique dans l’épididyme du rat des sables adulte, capturé dans la région de Beni Abbès, en Algérie, l’aromatase, l’œstradiol, les récepteurs des androgènes (RA) et des œstrogènes (REα, REβ, GPR30) ont été recherchés chez des animaux en saison d’activité, en saison de repos sexuel, chez des animaux castrés, castrés puis traités par la testostérone et chez des animaux ayant subi la ligature des canaux efférents. En saison d’activité, les RA sont ubiquitaires, l’aromatase est cytoplasmique par contre l’œstradiol est nucléaire et cytoplasmique. Les REα et le GPR30 sont principalement dans le cytoplasme apical par contre les REβ sont nucléaires. En saison de repos sexuel, les RA, l’aromatase, l’œstradiol, les REα et le GPR30 persistent, cependant, les REβ subissent une translocation cytoplasmique. Chez les animaux castrés, les RA, l’aromatase et l’œstradiol sont réduits par contre les REα persistent avec une faible intensité. Le GPR30 est cytoplasmique et nucléaire. Chez les animaux castrés puis traités, les RA, l’aromatase, l’œstradiol, les REα, les REβ et le GPR30 sont restaurés. Chez les animaux ligaturés, le RA est faiblement conservé uniquement dans l’épididyme proximal. L’aromatase et l’œstradiol sont conservés. Le signal des REα, des REβ et du GPR30 est fortement exprimé dans le noyau et le cytoplasme dans l’épididyme proximal par contre il est fortement exprimé uniquement pour les REα dans l’épididyme distal. Par western blot, les RA, REα, REβ et GPR30 sont de 122, 64, 55 et 55 kDa respectivement.

In order to highlight the main elements of androgen and estrogen pathway in the epididymis of sand rat, captured in Beni Abbès area, in Algeria, androgen receptor (AR), aromatase, estradiol, estrogen receptors (ERα, ERβ and GPR30) were explored in breeding season, in resting season and in animals underwent castration, castration then testosterone treatment and ligation of efferent ducts. In breeding season, AR has a ubiquitous distribution, aromatase is exclusively cytoplasmic and estradiol is nuclear and cytoplasmic. The ERα and GPR30 were distributed with a high intensity in the apical cytoplasm contrarily to ERβ which were nuclear. In resting season, AR, aromatase, estradiol, ERα persist with lower staining. However, ERβ undergo cytoplasmic translocation and GPR30 persist in cytoplasm. In castrated animals, AR, aromatase and estradiol are reduced. ERα persist with low intensity in the apical cytoplasm. GPR30 is distributed in the cytoplasm and the nucleus. In castrated then treated animals, AR is restored; aromatase and estradiol reappear with a cytoplasmic localization for aromatase, nuclear and apical for ERα. ERβ and GPR30 are restored and have a cytoplasmic localization. In ligatured, RA is preserved in the caput, aromatase and estradiol persist caput and cauda. The signal of ERα, ERβ and GPR30 is highly expressed in the nucleus and cytoplasm of caput epididymis and highly expressed of ERα exclusively in cauda. By Western blot,…

Advisors/Committee Members: Exbrayat, Jean-Marie (thesis director), Gernigon-Spychalowicz, Thérèse (thesis director).

Subjects/Keywords: P450 aromatase; GPR30; P450 aromatase; GPR30; Epididymis; Apoptosis; Estrogen Receptors; Androgen Receptors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Menad, R. (2015). Régulation fonctionnelle de l’épididyme d’un rongeur déserticole, Psammomys obesus, CRETZSCHMAR, 1828 : Functional Regulation of Epididymis of Sand Rat Psammomys obesus, CRETZSCHMAR, 1828. (Doctoral Dissertation). Paris, EPHE; Université des sciences et de la technologie Houari Boumediene (Alger). Retrieved from http://www.theses.fr/2015EPHE3011

Chicago Manual of Style (16^th Edition):

Menad, Rafik. “Régulation fonctionnelle de l’épididyme d’un rongeur déserticole, Psammomys obesus, CRETZSCHMAR, 1828 : Functional Regulation of Epididymis of Sand Rat Psammomys obesus, CRETZSCHMAR, 1828.” 2015. Doctoral Dissertation, Paris, EPHE; Université des sciences et de la technologie Houari Boumediene (Alger). Accessed January 25, 2021. http://www.theses.fr/2015EPHE3011.

MLA Handbook (7^th Edition):

Menad, Rafik. “Régulation fonctionnelle de l’épididyme d’un rongeur déserticole, Psammomys obesus, CRETZSCHMAR, 1828 : Functional Regulation of Epididymis of Sand Rat Psammomys obesus, CRETZSCHMAR, 1828.” 2015. Web. 25 Jan 2021.

Vancouver:

Menad R. Régulation fonctionnelle de l’épididyme d’un rongeur déserticole, Psammomys obesus, CRETZSCHMAR, 1828 : Functional Regulation of Epididymis of Sand Rat Psammomys obesus, CRETZSCHMAR, 1828. [Internet] [Doctoral dissertation]. Paris, EPHE; Université des sciences et de la technologie Houari Boumediene (Alger); 2015. [cited 2021 Jan 25]. Available from: http://www.theses.fr/2015EPHE3011.

Council of Science Editors:

Menad R. Régulation fonctionnelle de l’épididyme d’un rongeur déserticole, Psammomys obesus, CRETZSCHMAR, 1828 : Functional Regulation of Epididymis of Sand Rat Psammomys obesus, CRETZSCHMAR, 1828. [Doctoral Dissertation]. Paris, EPHE; Université des sciences et de la technologie Houari Boumediene (Alger); 2015. Available from: http://www.theses.fr/2015EPHE3011

University of Hong Kong

21. 魏娜. Oestrogen receptor subtypes in ovarian cancer.

Degree: 2008, University of Hong Kong

URL: http://hdl.handle.net/10722/52248

Subjects/Keywords: Ovaries - Cancer.; Estrogen - Receptors.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

魏娜. (2008). Oestrogen receptor subtypes in ovarian cancer. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/52248

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

魏娜. “Oestrogen receptor subtypes in ovarian cancer.” 2008. Thesis, University of Hong Kong. Accessed January 25, 2021. http://hdl.handle.net/10722/52248.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

魏娜. “Oestrogen receptor subtypes in ovarian cancer.” 2008. Web. 25 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

魏娜. Oestrogen receptor subtypes in ovarian cancer. [Internet] [Thesis]. University of Hong Kong; 2008. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10722/52248.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

魏娜. Oestrogen receptor subtypes in ovarian cancer. [Thesis]. University of Hong Kong; 2008. Available from: http://hdl.handle.net/10722/52248

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

22. Rita de Cassia Pereira da Costa e Silva. ANÁLISE DO POLIMOFISMO RsaI DO GENE RECEPTOR BETA ESTRÓGENO (REβ) EM MULHERES COM ENDOMETRIOSE.

Degree: 2010, Universidade Católica de Goiás

URL: http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=839

►

A endometriose é definida pelo aparecimento de focos de tecido endometrial com características glandulares e/ou estromais idênticos aos da cavidade uterina em outras localizações, que… (more)

▼

A endometriose é definida pelo aparecimento de focos de tecido endometrial com características glandulares e/ou estromais idênticos aos da cavidade uterina em outras localizações, que não o endométrio. E esses focos de tecido geralmente são funcionantes e sensíveis à ação de hormônios apresentando um forte componente genético correlacionando a doença com diversos polimorfismos. Incide principalmente em mulheres em idade reprodutiva. Está presente em 10% na população geral e com infertilidade em 30% a 40% dos casos. O grau do comprometimento da endometriose baseia-se num sistema de pontos proposta pela American Society for Reproductive Medicine (1978), com base nos achados de laparoscopia. A ação estrogênica é mediada por receptores intracelulares, que com a ligação dos ligantes são translocados para o núcleo onde ativam a transcrição gênica e o REβ é uma das isoformas destes receptores. O gene do REβ foi mapeado e localizado no braço longo do cromossomo 14 no loco 2 entre os sublocos 22 e 24 (14q22-24) e o polimorfismo consiste em uma transição silenciosa G1082A no domínio de ligação do éxon 5. A intenção deste estudo foi determinar a freqüência do polimorfismo RsaI do gene REβ, em dois grupos de pacientes com endometriose e sem sintomas da doença. O estudo incluiu 54 amostras de sangue periférico de mulheres com endometriose proveniente de um centro de referência em videolaparoscopia e infertilidade de Goiânia (FÉRTILE) e 46 amostras de sangue periférico de mulheres sem clínica de endometriose chamado de grupo controle. Posteriormente o grupo com endometriose foi subdividido em dois grupos férteis (n= 25) e inférteis (n= 27). O polimorfismo RsaI do gene REβ (AA, AG, GG) e do gene p53 no códon 72 foi avaliado por PCR. A freqüência do genótipo heterozigoto AG foi 9 vezes maior nas pacientes com endometriose (59,3%) do que no grupo controle (6,5%). As pacientes férteis (68%) apresentaram uma freqüência AG de 10,5 vezes maior que no grupo controle (6,5%) e as inférteis (55,6%) 8,5 vezes maior que no grupo controle. Dos hábitos sociais como o fumo, álcool, anticoncepcionais e atividade física não foi encontrado uma associação significante. Das pacientes com genótipos ArgPro/ProPro (51,5%) do polimorfismo do gene p53 a frequência AG foi 8,6 vezes maior do que no grupo controle (6%). Conclui-se que, a freqüência AG do polimorfismo RsaI do gene REβ está associada a presença da endometriose.

Endometriosis is defined by the appearance of foci of endometrial tissue with glandular features and / or stromal identical to the uterine cavity at locations other than the endometrium. And these foci of tissue are usually functioning and sensitive to the action of hormones showing a strong genetic component to disease correlated with several polymorphisms. It focuses primarily on women of reproductive age. Is present in 10% of general population and infertility in 30% to 40% of cases. The degree of involvement of endometriosis is based on a points system proposed by the American Society for Reproductive…

Advisors/Committee Members: Bárbara Mariotto Bordin, Flávia Melo Rodrigues, Waldemar Naves do Amaral, Katia Karina Verolli de Oliveira Moura.

Subjects/Keywords: endometriose; receptores estrógenos; infertilidade; GENETICA; endometriosis; estrogen receptors; infertility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, R. d. C. P. d. C. e. (2010). ANÁLISE DO POLIMOFISMO RsaI DO GENE RECEPTOR BETA ESTRÓGENO (REβ) EM MULHERES COM ENDOMETRIOSE. (Thesis). Universidade Católica de Goiás. Retrieved from http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=839

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Rita de Cassia Pereira da Costa e. “ANÁLISE DO POLIMOFISMO RsaI DO GENE RECEPTOR BETA ESTRÓGENO (REβ) EM MULHERES COM ENDOMETRIOSE.” 2010. Thesis, Universidade Católica de Goiás. Accessed January 25, 2021. http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=839.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Rita de Cassia Pereira da Costa e. “ANÁLISE DO POLIMOFISMO RsaI DO GENE RECEPTOR BETA ESTRÓGENO (REβ) EM MULHERES COM ENDOMETRIOSE.” 2010. Web. 25 Jan 2021.

Vancouver:

Silva RdCPdCe. ANÁLISE DO POLIMOFISMO RsaI DO GENE RECEPTOR BETA ESTRÓGENO (REβ) EM MULHERES COM ENDOMETRIOSE. [Internet] [Thesis]. Universidade Católica de Goiás; 2010. [cited 2021 Jan 25]. Available from: http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=839.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva RdCPdCe. ANÁLISE DO POLIMOFISMO RsaI DO GENE RECEPTOR BETA ESTRÓGENO (REβ) EM MULHERES COM ENDOMETRIOSE. [Thesis]. Universidade Católica de Goiás; 2010. Available from: http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=839

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Kamal, Nuzhat. Studies on estrogen uptake and estrogen receptors in the male genital organs; -.

Degree: Chemistry, 1983, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/53121

Abstract not available newline newline

Summary p. 1-6

Advisors/Committee Members: Setty, B S.

Subjects/Keywords: Estrogen; Receptors; Genital; Organs; Integrity

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kamal, N. (1983). Studies on estrogen uptake and estrogen receptors in the male genital organs; -. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/53121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kamal, Nuzhat. “Studies on estrogen uptake and estrogen receptors in the male genital organs; -.” 1983. Thesis, Aligarh Muslim University. Accessed January 25, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/53121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kamal, Nuzhat. “Studies on estrogen uptake and estrogen receptors in the male genital organs; -.” 1983. Web. 25 Jan 2021.

Vancouver:

Kamal N. Studies on estrogen uptake and estrogen receptors in the male genital organs; -. [Internet] [Thesis]. Aligarh Muslim University; 1983. [cited 2021 Jan 25]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kamal N. Studies on estrogen uptake and estrogen receptors in the male genital organs; -. [Thesis]. Aligarh Muslim University; 1983. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

24. Mortimer, Morne Francois. Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts.

Degree: MSc, Biochemistry, 2014, Stellenbosch University

URL: http://hdl.handle.net/10019.1/95748

► ENGLISH ABSTRACT: Phytoestrogens are perceived as a safer alternative to conventional hormone replacement therapy (HRT) for the alleviation of menopausal symptoms as they present a… (more)

▼ ENGLISH ABSTRACT: Phytoestrogens are perceived as a safer alternative to conventional hormone replacement therapy (HRT) for the alleviation of menopausal symptoms as they present a decreased side-effect profile. The Cyclopia subternata (honeybush) methanol extract, SM6Met, displays estrogenic attributes desirable for the development of an phytoestrogenic nutraceutical, namely, estrogen receptor (ER) α antagonism, ERβ agonism, and antagonism of 17β-estradiol (E2)-induced breast cancer cell proliferation. Activity-guided fractionation was employed in an attempt to isolate and identify the compounds inducing the specific estrogenic profile of SM6Met. Fractions were evaluated for estrogenic attributes and major polyphenols present. Initial liquid-liquid fractionation of SM6Met yielded a polar fraction (PF) and a non-polar fraction (NPF), with the estrogenic attributes of interest retained and concentrated in NPF. Subsequent high performance counter-current chromatography (HPCCC) fractionation of NPF yielded three fractions (F1-F3). Interestingly, the fractions revealed separation of the previously demonstrated positive estrogenic attributes of NPF into separate fractions, with F1 and F2 acting as ERα antagonists, only F2 inducing antagonism of E2-induced breast cancer cell proliferation and only F3 retaining ERβ agonist activity. Although ERβ agonism displayed by F3 was robust and significantly higher than that of 10-11 M E2, it also displayed weak ERα agonism. Fractionation also for the first time in the study revealed ERβ antagonism, as induced by F1. In terms of major polyphenols HPCCC fractionation resulted in a divergence with F1 emerging as the dihydrochalcone-rich fraction and F2 as the flavanone and benzophenone-rich fraction, while the xanthones, flavones and phenolic acids were retained in F3. In addition, a preliminary absorption study was conducted using the ex vivo flow-through diffusion assay whereby the permeability of porcine small and large intestine for polyphenols in SM6Met was evaluated. The major compounds present in SM6Met were not able to penetrate the large intestinal mucosa, but small intestinal permeation of all major compounds in SM6Met ensued, with apparent permeability coefficient (Papp) values ranging from 1.91-3.74 x 10-6 cm.s-1, indicative of good intestinal absorption. Open source programs used for theoretical prediction of absorption gave conflicting results, emphasising the need to confirm predictions experimentally. ACD/Labs predicted poor intestinal absorption of SM6Met compounds based on physicochemical profiling, while OSIRIS and ChemAxon anticipated good absorption. In conclusion, activity-guided fractionation results suggest that retention of all the positive estrogenic attributes of the original SM6Met in one fraction is not an attainable goal. This suggests that several of the polyphenols present in SM6Met or NPF, through antagonistic, synergistic, or additive effects, may together be conferring these desired estrogenic traits. Thus production or isolation of a mixture of… Advisors/Committee Members: Louw, Ann, Joubert, Elizabeth, Stellenbosch University. Faculty of Science. Dept. of Biochemistry..

Subjects/Keywords: Phytoestrogens; Honeybush – Physiology; Estrogen receptors; Breast cancer – Prevention; UCTD

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APA (6^th Edition):

Mortimer, M. F. (2014). Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/95748

Chicago Manual of Style (16^th Edition):

Mortimer, Morne Francois. “Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts.” 2014. Masters Thesis, Stellenbosch University. Accessed January 25, 2021. http://hdl.handle.net/10019.1/95748.

MLA Handbook (7^th Edition):

Mortimer, Morne Francois. “Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts.” 2014. Web. 25 Jan 2021.

Vancouver:

Mortimer MF. Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts. [Internet] [Masters thesis]. Stellenbosch University; 2014. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10019.1/95748.

Council of Science Editors:

Mortimer MF. Isolation and identification of compounds conferring phytoestrogenic activity to Cyclopia extracts. [Masters Thesis]. Stellenbosch University; 2014. Available from: http://hdl.handle.net/10019.1/95748

Freie Universität Berlin

25. Dworatzek, Elke. Role of 17ß-estradiol and estrogen receptors in sex-specific cardiac remodeling.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-27304

► Zahlreiche Studien weisen auf Geschlechterunterschiede in den kardialen Umbauprozessen in der physiologischen und pathologischen Myokardhypertrophie hin. Die Forschungsergebnisse der vorliegenden Habilitationsschrift tragen dazu bei, weitere… (more)

▼ Zahlreiche Studien weisen auf Geschlechterunterschiede in den kardialen Umbauprozessen in der physiologischen und pathologischen Myokardhypertrophie hin. Die Forschungsergebnisse der vorliegenden Habilitationsschrift tragen dazu bei, weitere Details zu den dafür verantwortlichen geschlechtsspezifischen molekularen Mechanismen aufzuzeigen. Außerdem gelang es mit Hilfe der durchgeführten Untersuchungen, die regulatorische Rolle von 17-Östradiol (E2) sowie den Östrogenrezeptoren alpha (ERα) und beta (ERβ) in beiden Geschlechtern sowohl unter physiologischen als auch pathologischen Umständen genauer zu definieren. Im Rahmen der durchgeführten Untersuchungen der vorliegenden Arbeit konnten wir zunächst, über bereits beschriebene Mechanismen hinaus, weitere verantwortliche Signalwege zur geschlechtsdimorphen Entwicklung der trainingsinduzierten physiologischen Myokardhypertrophie am Tiermodell identifizieren. Weiterhin zeigten wir Unterschiede in der kardialen mitochondrialen Adaption zwischen weiblichen und männlichen Mäusen, und es gelang uns in beiden Geschlechtern die modulierende Rolle von ERβ bei der Ausprägung der trainingsinduzierten physiologischen Myokardhypertrophie aufzudecken. Darüber hinaus beschrieben wir mit Hilfe eines Vergleiches verschiedener Altersstufen beider Geschlechter erstmalig Unterschiede zwischen Männern und Frauen im altersbedingten Umbau der kardialen extrazellulären Matrix. Hierbei zeigten sich mit zunehmendem Alter eine Abnahme ausgewählter extrazellulären Matrixproteine in den Herzen der Männer und ein Anstieg dieser Proteine in den weiblichen Herzen. Eine unveränderte Kollagenmenge im Herzen weist darauf hin, dass es sich bei den altersabhängigen Veränderungen der extrazellulären Matrixproteine um einen physiologischen Adaptionsprozess des alternden Herzens handelt. Dagegen konnte die Existenz signifikanter Geschlechterunterschiede in der Entstehung der kardialen Fibrose in Patienten mit Aortenstenose im Rahmen der durchgeführten Arbeiten belegt werden. Frauen zeigen hierbei eine deutlich geringere Aktivierung pro-fibrotischer Signalmechanismen, die mit einer verringerten Expression von fibrotischen Schlüsselregulatoren und einer geringeren Kollagenablagerung im Gewebe einhergeht. Zudem konnten wir eine signifikante Unterdrückung von Zytokin-, Chemokin- und Inflammations- assoziierten Wegen in den Herzen der Frauen mit Aortenstenose nachweisen. Weiterhin zeigten wir, dass Frauen, im Vergleich zu Männern, eine schnellere post-operative Regression der Myokardhypertrophie und eine bessere Überlebensrate nach Aortenklappenersatz. Diese Ergebnisse belegen, dass es geschlechtsspezifische Unterschiede in der Anpassungsreaktion, sowohl unter Belastung als auch bei Entlastung des Herzens, gibt. In beiden Fällen spielt die kardiale Fibrose dabei eine wichtige Rolle. In einer weiteren Untersuchung identifizierten und analysierten wir die verantwortlichen Mechanismen für die Geschlechterunterschiede in der kardialen Fibrose näher. Dabei gelang es uns, die modulierende Rolle von E2 und den ER in… Advisors/Committee Members: female (gender), Wieland, Thomas (firstReferee), Lorenz, Kristina (furtherReferee).

Subjects/Keywords: cardiac remodeling; sex differences; 17ß-estradiol; estrogen receptors; ddc:610

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APA (6^th Edition):

Dworatzek, E. (2020). Role of 17ß-estradiol and estrogen receptors in sex-specific cardiac remodeling. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dworatzek, Elke. “Role of 17ß-estradiol and estrogen receptors in sex-specific cardiac remodeling.” 2020. Thesis, Freie Universität Berlin. Accessed January 25, 2021. http://dx.doi.org/10.17169/refubium-27304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dworatzek, Elke. “Role of 17ß-estradiol and estrogen receptors in sex-specific cardiac remodeling.” 2020. Web. 25 Jan 2021.

Vancouver:

Dworatzek E. Role of 17ß-estradiol and estrogen receptors in sex-specific cardiac remodeling. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Jan 25]. Available from: http://dx.doi.org/10.17169/refubium-27304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dworatzek E. Role of 17ß-estradiol and estrogen receptors in sex-specific cardiac remodeling. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Houston

26. Katchy, Anne Chinenye 1984-. Exploring estrogen receptor gene regulatory mechanism in breast cancer.

Degree: PhD, Biochemistry, 2013, University of Houston

URL: http://hdl.handle.net/10657/1061

► Estrogen has vital roles in development and maintenance of mammary gland and supports the growth of the majority of primary breast cancer. It carries out… (more)

▼ Estrogen has vital roles in development and maintenance of mammary gland and supports the growth of the majority of primary breast cancer. It carries out its function through the estrogen receptors (ER): ERα and ERβ. In this dissertation, we focused on identifying the functional and genome wide effects of both receptors in breast cancer cell lines (T47D and MCF7). First, we aimed at identifying microRNAs (miRNAs) that are significantly associated with normal or disrupted estrogen signaling in breast cancer cells, and are regulated by ERα and ERβ. Despite the fact that 24h estrogen treatment results in strong changes to expression of about 900 protein-coding transcripts, we found no significant changes in mature miRNA expression levels by 17β-estradiol (E2)-activated ERα in neither T47D nor MCF7 breast cancer cells. On the other hand, when studying the effects of exogenously expressed ERβ, we identified miR-135a, miR-21, miR-200c, and miR-522, among others, as being regulated. Most of the ERβ effects were ligand-independent, but we observed a significant response to E2 in the MCF7-ERβ cells. Also, the MCF7-ERβ showed enhanced stem cell abilities in comparison to the MCF7-Control cells as illustrated by increased mammospheres formation during several generations. Secondly, we aimed at investigating environmental factors and its effect on the risk for breast cancer. We identified the gene expression response for each of these compounds: BPA, genistein (Gen), E2, and soy formula extract (SF) in MCF7 cells, and found that each regulated similar genes in the same manner. Many target genes regulated by BPA, Gen, and SF, were involved in important biological processes identical to those of estrogen. Investigation of non-ERα mediated regulations for these EDCs suggested that these compounds may regulate gene transcription solely through ERα, in the cells and doses used. Furthermore, we found that these compounds acted together in a sub-additive manner, and tumor clustering with the gene expression profile of these EDC compounds revealed a significantly lower disease free survival. Altogether, the data presented in this dissertation would aid in increasing the knowledge of early risk factors for breast cancer. Our work provides data for future use of the estrogen receptors in clinical applications by providing new candidates for pharmaceutical drug development, as well as, biomarkers for diagnosis and prognosis. Advisors/Committee Members: Williams, Cecilia M. (advisor), Schwartz, Robert J. (committee member), Webb, Paul (committee member), Gao, Xiaolian (committee member).

Subjects/Keywords: Estrogen receptors; MicroRNAs (miRNA); Breast cancer; Bisphenol A; Phytoestrogen; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Katchy, A. C. 1. (2013). Exploring estrogen receptor gene regulatory mechanism in breast cancer. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/1061

Chicago Manual of Style (16^th Edition):

Katchy, Anne Chinenye 1984-. “Exploring estrogen receptor gene regulatory mechanism in breast cancer.” 2013. Doctoral Dissertation, University of Houston. Accessed January 25, 2021. http://hdl.handle.net/10657/1061.

MLA Handbook (7^th Edition):

Katchy, Anne Chinenye 1984-. “Exploring estrogen receptor gene regulatory mechanism in breast cancer.” 2013. Web. 25 Jan 2021.

Vancouver:

Katchy AC1. Exploring estrogen receptor gene regulatory mechanism in breast cancer. [Internet] [Doctoral dissertation]. University of Houston; 2013. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10657/1061.

Council of Science Editors:

Katchy AC1. Exploring estrogen receptor gene regulatory mechanism in breast cancer. [Doctoral Dissertation]. University of Houston; 2013. Available from: http://hdl.handle.net/10657/1061

Michigan State University

27. Kramer, Vincent J. (Vincent Joseph). Xeno-estrogens in the aquatic environment : development and application of in vitro and in vivo bioassays.

Degree: PhD, Department of Fisheries and Wildlife and the Institute for Environmental Toxicology, 1996, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:26297

Subjects/Keywords: Estrogen – Receptors; Biological assay

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APA (6^th Edition):

Kramer, V. J. (. J. (1996). Xeno-estrogens in the aquatic environment : development and application of in vitro and in vivo bioassays. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:26297

Chicago Manual of Style (16^th Edition):

Kramer, Vincent J (Vincent Joseph). “Xeno-estrogens in the aquatic environment : development and application of in vitro and in vivo bioassays.” 1996. Doctoral Dissertation, Michigan State University. Accessed January 25, 2021. http://etd.lib.msu.edu/islandora/object/etd:26297.

MLA Handbook (7^th Edition):

Kramer, Vincent J (Vincent Joseph). “Xeno-estrogens in the aquatic environment : development and application of in vitro and in vivo bioassays.” 1996. Web. 25 Jan 2021.

Vancouver:

Kramer VJ(J. Xeno-estrogens in the aquatic environment : development and application of in vitro and in vivo bioassays. [Internet] [Doctoral dissertation]. Michigan State University; 1996. [cited 2021 Jan 25]. Available from: http://etd.lib.msu.edu/islandora/object/etd:26297.

Council of Science Editors:

Kramer VJ(J. Xeno-estrogens in the aquatic environment : development and application of in vitro and in vivo bioassays. [Doctoral Dissertation]. Michigan State University; 1996. Available from: http://etd.lib.msu.edu/islandora/object/etd:26297

University of Florida

28. Bean, Linda A. Estrogen Receptors, Memory and the Closing of the Therapeutic Window.

Degree: PhD, Medical Sciences - Neuroscience (IDP), 2015, University of Florida

URL: https://ufdc.ufl.edu/UFE0049114

Subjects/Keywords: aging; estrogen; memory; receptors

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APA (6^th Edition):

Bean, L. A. (2015). Estrogen Receptors, Memory and the Closing of the Therapeutic Window. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0049114

Chicago Manual of Style (16^th Edition):

Bean, Linda A. “Estrogen Receptors, Memory and the Closing of the Therapeutic Window.” 2015. Doctoral Dissertation, University of Florida. Accessed January 25, 2021. https://ufdc.ufl.edu/UFE0049114.

MLA Handbook (7^th Edition):

Bean, Linda A. “Estrogen Receptors, Memory and the Closing of the Therapeutic Window.” 2015. Web. 25 Jan 2021.

Vancouver:

Bean LA. Estrogen Receptors, Memory and the Closing of the Therapeutic Window. [Internet] [Doctoral dissertation]. University of Florida; 2015. [cited 2021 Jan 25]. Available from: https://ufdc.ufl.edu/UFE0049114.

Council of Science Editors:

Bean LA. Estrogen Receptors, Memory and the Closing of the Therapeutic Window. [Doctoral Dissertation]. University of Florida; 2015. Available from: https://ufdc.ufl.edu/UFE0049114

University of Illinois – Urbana-Champaign

29. Charn, Tze Howe. An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells.

Degree: PhD, 0408, 2011, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/18589

► The nuclear hormone receptors, ER?? and ER??, are known to regulate the transcriptional response programs of their target cells, including breast cancer cells. However, their… (more)

▼ The nuclear hormone receptors, ER?? and ER??, are known to regulate the transcriptional response programs of their target cells, including breast cancer cells. However, their comparative abilities to localize at chromatin binding sites across the genome, and the recruitment of major coregulators such as SRC3 and RIP140 by the ERs, and the association of ERs with other transcription factors and chromatin remodeling factors is incompletely understood. Therefore, in this report, we have used both chromatin immunoprecipitation (ChIP) on microarray (ChIP-chip) and ChIP sequencing (ChIP-seq) approaches in breast cancer cells containing three different complements of ERs (ER?? alone, ER?? alone, or ER?? + ER??) treated with estradiol to define the cartography of chromatin binding sites for ER??, ER??, and the coregulators SRC3 and RIP140. We found that ER?? and ER?? bind to a similar, large number of sites in breast cancer cells containing only one ER subtype, but the two ERs appear to restrict each others chromatin binding and occupy fewer sites in cells containing both ER?? and ER??. We also observed that there are differences in term of enriched motifs in ER?? and ER?? binding sites, with ER?? binding sites enriched in GATA and FOXA1 motifs, whereas ER?? sites being preferentially enriched in E2F motifs. In addition, in cells containing both ER?? and ER??, ER?? appears to displace ER?? so that ER?? binds to sites substantially less enriched in estrogen response element (ERE) sequence motifs. Gene chip microarray transcriptional profiling and gene ontology analysis delineated a core set of genes that correlate with ER?? proliferative and ER?? anti-proliferative effects in breast cancer cells. ER?? activation by estradiol was associated with the inhibition of genes associated with cell proliferation and the up-regulation of pro-apoptotic genes and genes responding to DNA damage, whereas ER?? activation was associated with the downregulation of pro-apoptotic genes and genes repressing transcription. Analysis of chromatin binding of SRC3 and RIP140 by ChIP-seq revealed that these coregulators are recruited preferentially to ER binding sites of estrogen-induced genes, whereas they are seldom recruited to ER binding sites of hormone-repressed genes, indicating that the SRC3-RIP140 complex is likely to be playing a central role in the induction of ER targeted genes. Our findings suggest an integrated model in which the actions of cofactors such as FOXA1, GATA3, and E2F enforce the selectivity and range of ER?? and ER?? binding and gene regulatory actions, with the coregulators SRC3 and RIP140 preferentially supporting the stimulatory actions of both receptors on gene expression. Advisors/Committee Members: Katzenellenbogen, John A. (advisor), Katzenellenbogen, John A. (Committee Chair), Katzenellenbogen, Benita S. (committee member), Zhong, Sheng (committee member), Wang, Yingxiao (committee member).

Subjects/Keywords: Estrogen receptors; MCF-7; nuclear receptor coactivator 3 (SRC3); RIP140

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APA (6^th Edition):

Charn, T. H. (2011). An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/18589

Chicago Manual of Style (16^th Edition):

Charn, Tze Howe. “An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells.” 2011. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 25, 2021. http://hdl.handle.net/2142/18589.

MLA Handbook (7^th Edition):

Charn, Tze Howe. “An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells.” 2011. Web. 25 Jan 2021.

Vancouver:

Charn TH. An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2011. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/2142/18589.

Council of Science Editors:

Charn TH. An integrated network of estrogen receptors alpha and beta, and coregulators, for deciphering estrogen signaling in breast cancer cells. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2011. Available from: http://hdl.handle.net/2142/18589

University of Minnesota

30. Triemstra, Jennifer Lynn. Sex differences and the role of estrogen, estrous cycle, and DRG neurons in a mouse model of cancer pain.

Degree: PhD, Comparative and Molecular Biosciences, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/163924

► While conflicting views exist in the literature regarding sex differences in cancer pain, recent studies show that women are more likely to experience greater cancer… (more)

▼ While conflicting views exist in the literature regarding sex differences in cancer pain, recent studies show that women are more likely to experience greater cancer pain than men. Transient receptor potential (TRP) are considered critical receptors in tumor-induced peripheral sensitization while cycling sex steroid hormones are considered critical factors in sex-dependent differences in cancer pain. Whether sex differences in cancer pain are due to estrogens affect on TRP receptors has yet to be determined. Utilizing a mouse model of bone cancer, we compared tumor-induced mechanical allodynia and thermal (heat and cold) hyperalgesia in intact, gonadectomized, and estradiol-replaced males and females. Tumor-induced changes in TRP and estrogen receptor mRNA levels were assessed. We also examined the anti-nociceptive effects of a topical application of a TRPM8 agonist (menthol) and subcutaneous injected TRPV1 antagonist on tumor-induced nociception. We found no sex differences in tumor-induced mechanical allodynia, but, there was greater mechanical allodynia in females during proestrus/estrus than during diestrus. Estradiol replacement in gonadectomized females increased tumor-induced mechanical allodynia, but decreased allodynia in gonadectomized males. Collectively, females were more sensitive to tumor-induced cold hyperalgesia than males. Tumor-induced cold sensitivity was increased in all gonadectomized animals regardless of estradiol replacement. GPR30 mRNA was greater in females during diestrus than proestrus/estrus. TRPV1 mRNA was lower in diestrus females but greater during proestrus/estrus. Estradiol replacement had in inverse effect on TRPV1 mRNA expression in gonadectomized females, but had no effect in gonadectomized males. TRPM8 mRNA was greater in females than males, while TRPA1 mRNA was greater in males than in females. The TRPV1 antagonist induced antinociception in proestrus/estrus females, but this effect was reduced in estradiol replaced gonadectomized males. Menthol-induced antinociception was reduced in gonadectomized females, but restored with estradiol replacement. These findings suggest that estrogen mediates sex differences in tumor-induced mechanical allodynia and cold hyperalgesia, TRP expression, menthol-induced antinociception as well as TRPV1 antagonist antinociception. Collectively, this verifies that estrogen is, in part, responsible for contrasting sensitivity of males and females to cancer pain.

Subjects/Keywords: Cancer pain; DRG neurons; Estrogen; Mouse; Sex differences; TRP receptors

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APA (6^th Edition):

Triemstra, J. L. (2014). Sex differences and the role of estrogen, estrous cycle, and DRG neurons in a mouse model of cancer pain. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/163924

Chicago Manual of Style (16^th Edition):

Triemstra, Jennifer Lynn. “Sex differences and the role of estrogen, estrous cycle, and DRG neurons in a mouse model of cancer pain.” 2014. Doctoral Dissertation, University of Minnesota. Accessed January 25, 2021. http://hdl.handle.net/11299/163924.

MLA Handbook (7^th Edition):

Triemstra, Jennifer Lynn. “Sex differences and the role of estrogen, estrous cycle, and DRG neurons in a mouse model of cancer pain.” 2014. Web. 25 Jan 2021.

Vancouver:

Triemstra JL. Sex differences and the role of estrogen, estrous cycle, and DRG neurons in a mouse model of cancer pain. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/11299/163924.

Council of Science Editors:

Triemstra JL. Sex differences and the role of estrogen, estrous cycle, and DRG neurons in a mouse model of cancer pain. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/163924

Open Access Theses and Dissertations