subject:(Escherichia coli)

California State University – East Bay

1. Herbold, Nicole M. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.

Degree: MSin Biological Sciences, 2014, California State University – East Bay

URL: http://hdl.handle.net/10211.3/130388

► There is a continuous need for improved accuracy and rapidity for detection methods applicable to foodborne pathogens; especially non-O157 Escherichia coli. Many E. coli, such… (more)

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Herbold, N. M. (2014). Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. (Masters Thesis). California State University – East Bay. Retrieved from http://hdl.handle.net/10211.3/130388

Chicago Manual of Style (16^th Edition):

Herbold, Nicole M. “Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.” 2014. Masters Thesis, California State University – East Bay. Accessed February 22, 2020. http://hdl.handle.net/10211.3/130388.

MLA Handbook (7^th Edition):

Herbold, Nicole M. “Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.” 2014. Web. 22 Feb 2020.

Vancouver:

Herbold NM. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. [Internet] [Masters thesis]. California State University – East Bay; 2014. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/10211.3/130388.

Council of Science Editors:

Herbold NM. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. [Masters Thesis]. California State University – East Bay; 2014. Available from: http://hdl.handle.net/10211.3/130388

Universidad de Chile

2. Martínez Vargas, Pámela Patricia. Alimentación cruzada ("cross-feeding") y dinámicas de diversidad en fase estacionaria de largo plazo en Escherichia coli : una aproximación matemática .

Degree: 2010, Universidad de Chile

URL: http://www.repositorio.uchile.cl/handle/2250/115640

► Diversas investigaciones han mostrado que cultivos microbianos provenientes de una cepa pura pueden dar paso gradualmente a la emergencia y mantención de diversidad genética en… (more)

▼ Diversas investigaciones han mostrado que cultivos microbianos provenientes de una cepa pura pueden dar paso gradualmente a la emergencia y mantención de diversidad genética en condiciones de privación de recursos. En fase estacionaria de largo plazo, en Escherichia coli, se ha observado que aquellas mutantes con mayor adecuación biológica son capaces de invadir el sistema (selección periódica), lo cual posteriormente da paso a una fase de coexistencia y como consecuencia la biodiversidad se ve incrementada. En este trabajo se postula que el mecanismo responsable de ambas dinámicas, selección periódica y coexistencia, es el cross-feeding, en otras palabras la existencia de acoplamientos entre los requerimientos de nutrientes por parte de las mutantes, tal que los productos de excreción de un genotipo son los recursos de otro. El objetivo de esta tesis fue estudiar el rol de este mecanismo en las dinámicas de diversidad observadas en fase estacionaria de largo plazo, a través de la generación de un modelo de simulación matemático. En el modelo propuesto, las mutaciones le confieren a los individuos la capacidad de metabolizar y excretar distintos metabolitos, donde la supervivencia y la reproducción son dependientes de los recursos requeridos por cada genotipo. Nuestros resultados muestran que bajo condiciones limitadas de nutrientes, en ausencia de cross-feeding, la población muere rápidamente. Sin embargo, la presencia de cross-feeding produjo dinámicas de diversidad similares a las descritas en cultivos de largo plazo (selección periódica y coexistencia). Estos resultados indican que este mecanismo podría ser fundamental en la persistencia de las poblaciones por largos periodos de tiempo y que jugaría un rol importante en la emergencia y mantención de diversidad en ecosistemas microbianos.; Several studies have shown that microbial cultures from a pure strain may gradually lead to the emergence and maintenance of genetic diversity in deprivation of resources. Long-term stationary phase cultures of Escherichia coli have shown that these mutants with higher fitness are able to invade the system (periodic selection), but then these mutants do not exclude each other and as a result biodiversity increases. This work argues that the mechanism responsible for both dynamics, periodic selection and coexistence, is cross-feeding; in other words the existence of links between nutrient requirements by the mutants, in such a way that excretory products of one genotype are the resources of another. The aim of this thesis was to study the role of this mechanism in diversity dynamics observed in long-term stationary phase, through the generation of a mathematical simulation model. In this model, mutations grant individuals the ability to metabolize and excrete different metabolites, where survival and reproduction are dependent on the resources required by each genotype. Our results show that under limited concentrations of nutrients and without recycling of metabolites, the population quickly dies. However, the presence of… Advisors/Committee Members: Zaldívar San Román, María Mercedes (advisor), Marquet, Pablo A (advisor), Fuentes, Miguel A (advisor).

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Martínez Vargas, P. P. (2010). Alimentación cruzada ("cross-feeding") y dinámicas de diversidad en fase estacionaria de largo plazo en Escherichia coli : una aproximación matemática . (Thesis). Universidad de Chile. Retrieved from http://www.repositorio.uchile.cl/handle/2250/115640

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martínez Vargas, Pámela Patricia. “Alimentación cruzada ("cross-feeding") y dinámicas de diversidad en fase estacionaria de largo plazo en Escherichia coli : una aproximación matemática .” 2010. Thesis, Universidad de Chile. Accessed February 22, 2020. http://www.repositorio.uchile.cl/handle/2250/115640.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martínez Vargas, Pámela Patricia. “Alimentación cruzada ("cross-feeding") y dinámicas de diversidad en fase estacionaria de largo plazo en Escherichia coli : una aproximación matemática .” 2010. Web. 22 Feb 2020.

Vancouver:

Martínez Vargas PP. Alimentación cruzada ("cross-feeding") y dinámicas de diversidad en fase estacionaria de largo plazo en Escherichia coli : una aproximación matemática . [Internet] [Thesis]. Universidad de Chile; 2010. [cited 2020 Feb 22]. Available from: http://www.repositorio.uchile.cl/handle/2250/115640.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martínez Vargas PP. Alimentación cruzada ("cross-feeding") y dinámicas de diversidad en fase estacionaria de largo plazo en Escherichia coli : una aproximación matemática . [Thesis]. Universidad de Chile; 2010. Available from: http://www.repositorio.uchile.cl/handle/2250/115640

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

3. Jang, Hye In, 1985-. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.

Degree: PhD, Food Science, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/

►

Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts… (more)

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Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts for pathogens. In order to enhance safety of fresh produce, it is important to understand the interactions between human enteric pathogens and plants. However, little information is available about the behavior of human enteric pathogens on plants, such as mechanisms of survival and persistence. In this study, we investigated survival and persistence of Escherichia coli O157:H7 and O104:H4 strains on Arabidopsis thaliana and romaine lettuce, as well as production of capsular polysaccharide (CPS) and induction of plant defense response. Colonization study with E. coli O157:H7 86-24 wild-type strain and its isogenic mutants of surface polysaccharides showed that colanic acid-deficient and lipopolysaccharide (LPS)-deficient mutants significantly less survived on Arabidopsis plant and lettuce on day 1 and 5 post-inoculation, compared to the wild-type. The two mutants of colanic acid and LPS induced 2-fold greater PR1 gene expression and produced significantly lower amount of CPS compared to wild-type strain (P < 0.05). The results may suggest that structures of colanic acid and LPS of E. coli O157:H7 influence the plant defense response, thereby resulting in different survival and colonization on plants. To investigate fitness of an emerging Shiga toxin-producing E. coli (STEC), colonization of E. coli O104:H4 strains on plants were compared with of that of E. coli O157:H7 strains. Results showed that E. coli O104:H4 strains (RG1, C3493, and LpfA) significantly survived better than E. coli O157:H7 strains (7386 and sakai) on Arabidopsis plant and lettuce at day 5, with greater production of CPS and lower expression of PR1 gene (P < 0.05). These results indicate that different level of plant defense response and CPS production may have an impact on survival or fitness of E. coli O104:H4 and O157:H7 on plants. In order to develop control strategies in crop cultivation environments, it is essential to learn about the behavior of human enteric pathogens on plants, particularly factors influencing the ability of pathogen to overcome plant host immunity. The present study provides better understanding of roles of plant defense response and surface polysaccharides on the molecular interactions between human pathogens and plants. Interestingly, the similar trend of bacterial survival/persistence between Arabidopsis (model plant) and lettuce (plant crop) may suggest a potential use of Arabidopsis as an appropriate model plant for studying the mechanisms of plant responses to human enteric pathogens on leafy vegetables. This study also provides an insight into potential roles of CPS in the survival of human enteric pathogens.

Advisors/Committee Members: Matthews, Karl R (chair), School of Graduate Studies.

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Jang, Hye In, 1. (2017). Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55512/

Chicago Manual of Style (16^th Edition):

Jang, Hye In, 1985-. “Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.” 2017. Doctoral Dissertation, Rutgers University. Accessed February 22, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/55512/.

MLA Handbook (7^th Edition):

Jang, Hye In, 1985-. “Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.” 2017. Web. 22 Feb 2020.

Vancouver:

Jang, Hye In 1. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2020 Feb 22]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/.

Council of Science Editors:

Jang, Hye In 1. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/

Universidad de Cantabria

4. Márquez López, Alicia. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.

Degree: 2016, Universidad de Cantabria

URL: http://hdl.handle.net/10902/8428

► RESUMEN: Caracterización molecular de aislamientos de Escherichia coli procedentes de distintas muestras clínicas para tratar de evaluar la relación entre la resistencia a quinolonas y… (more)

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Márquez López, A. (2016). Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. (Doctoral Dissertation). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/8428

Chicago Manual of Style (16^th Edition):

Márquez López, Alicia. “Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.” 2016. Doctoral Dissertation, Universidad de Cantabria. Accessed February 22, 2020. http://hdl.handle.net/10902/8428.

MLA Handbook (7^th Edition):

Márquez López, Alicia. “Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.” 2016. Web. 22 Feb 2020.

Vancouver:

Márquez López A. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. [Internet] [Doctoral dissertation]. Universidad de Cantabria; 2016. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/10902/8428.

Council of Science Editors:

Márquez López A. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. [Doctoral Dissertation]. Universidad de Cantabria; 2016. Available from: http://hdl.handle.net/10902/8428

University of Aberdeen

5. Solecki, Olivia. Explaining the urban and rural differences of Escherichia coli 0157 human infection in Grampian.

Degree: 2008, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25203 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499729

► The rural population of Grampian was reported to be two times more likely to suffer from E. coli O157 infection than the urban population. E.… (more)

Subjects/Keywords: 616.9; Escherichia coli infections : Escherichia coli

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APA (6^th Edition):

Solecki, O. (2008). Explaining the urban and rural differences of Escherichia coli 0157 human infection in Grampian. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25203 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499729

Chicago Manual of Style (16^th Edition):

Solecki, Olivia. “Explaining the urban and rural differences of Escherichia coli 0157 human infection in Grampian.” 2008. Doctoral Dissertation, University of Aberdeen. Accessed February 22, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25203 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499729.

MLA Handbook (7^th Edition):

Solecki, Olivia. “Explaining the urban and rural differences of Escherichia coli 0157 human infection in Grampian.” 2008. Web. 22 Feb 2020.

Vancouver:

Solecki O. Explaining the urban and rural differences of Escherichia coli 0157 human infection in Grampian. [Internet] [Doctoral dissertation]. University of Aberdeen; 2008. [cited 2020 Feb 22]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25203 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499729.

Council of Science Editors:

Solecki O. Explaining the urban and rural differences of Escherichia coli 0157 human infection in Grampian. [Doctoral Dissertation]. University of Aberdeen; 2008. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25203 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499729

6. Renata Katsuko Takayama Kobayashi. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.

Degree: 2006, Universidade Estadual de Londrina

URL: http://189.90.64.145/document/?code=vtls000112627

►

Escherichia coli is an important agent of extra-intestinal infections both in humans and animals alike. In poultry, it is responsible for significant economic loss, being… (more)

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Escherichia coli is an important agent of extra-intestinal infections both in humans and animals alike. In poultry, it is responsible for significant economic loss, being known as the pathogenic avian E. coli (APEC); and it causes colibacillosis, which starts as an infection of the respiratory tract evolving to airsacculitis, pericarditis, perihepatitis and septicemia. Several virulence genes are involved, among which tsh. The tsh gene codifies the autotransporter protein temperature sensitive hemagglutinin (Tsh), which has hemagglutinating and proteolytic activities. Tsh is synthesized as 140 kDa precursor protein, whose processing results in 106 kDa passenger domain (Tshs) and 33 kDa beta-domain (Tshb). In our study, we observed that these proteins presence is dependent on temperature and on the medium utilized for bacterial growth. We also verified that both the recombinant Tsh (140 kDa) and the pellets from wild sample APEC 13, which contain Tshb (33 kDa), agglutinated chicken erythrocytes. Both the recombinant Tsh (140kDa) and the supernatants from APEC 13, which contain Tshs (106 kDa), caused proteolysis of mucin. Chicken anti-Tsh serum inhibited the hemagglutinating activity of strains APEC 13 and recombinant E. coli BL21/pET101-tsh, and it also inhibited the mucinolytic activity of Tsh protein. The proof of Tsh protein mucinolytic activity was achieved by means of SDS-PAGE in gels copolymerized with mucins from different sources.

Escherichia coli é um agente importante de infecções extraintestinais em humanos e em animais. Em aves, é responsável por grandes perdas econômicas, sendo conhecida como E. coli patogênica para aves (APEC), e causa a colibacilose, a qual inicia como infecção do trato respiratório e evolui para aerosaculite, pericardite, perihepatite e septicemia. Vários genes de virulência estão envolvidos, sendo tsh um deles. O gene tsh codifica a proteína autotransportada hemaglutinina temperatura sensível (Tsh), que tem atividade hemaglutinante e proteolítica. Tsh é sintetizada como uma proteína precursora de 140 kDa, que é clivada, em um domínio passageiro de 106 kDa (Tshs) e um beta-domínio de 33 kDa (Tshb). Em nosso trabalho observamos que a presença destas proteínas é dependente da temperatura e do meio de cultura utilizado para o crescimento bacteriano. Observamos ainda que, tanto a Tsh recombinante (140 kDa) quanto o sedimento da amostra selvagem APEC 13, que contém Tshb (33 kDa), aglutinaram eritrócitos de galinha. Tanto a Tsh recombinante (140kDa) quanto o sobrenadante da APEC 13, que contém Tshs (106 kDa), causam proteólise da mucina. O anti-Tsh do soro de galinha inibiu a hemaglutinação da APEC 13 e da recombinante E. coli BL21/pET101-tsh, e inibiu também a atividade mucinolítica da proteína Tsh. A comprovação da atividade mucinolítica da proteína Tsh, foi possível através de SDS-PAGE em géis copolimerizados com mucinas de diferentes origens.

Advisors/Committee Members: Luiz Carlos Jabur Gaziri ., Ermerson José Venancio, Halha Ostrensky Saridakis, Ionice Felipe, Marilda Carlos Vidotto.

Subjects/Keywords: Escherichia coli; Escherichia coli; Microbiologia; Microbiology

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APA (6^th Edition):

Kobayashi, R. K. T. (2006). Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. (Thesis). Universidade Estadual de Londrina. Retrieved from http://189.90.64.145/document/?code=vtls000112627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kobayashi, Renata Katsuko Takayama. “Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.” 2006. Thesis, Universidade Estadual de Londrina. Accessed February 22, 2020. http://189.90.64.145/document/?code=vtls000112627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kobayashi, Renata Katsuko Takayama. “Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.” 2006. Web. 22 Feb 2020.

Vancouver:

Kobayashi RKT. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. [Internet] [Thesis]. Universidade Estadual de Londrina; 2006. [cited 2020 Feb 22]. Available from: http://189.90.64.145/document/?code=vtls000112627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kobayashi RKT. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. [Thesis]. Universidade Estadual de Londrina; 2006. Available from: http://189.90.64.145/document/?code=vtls000112627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

7. Harding, Amanda. Mechanisms of acid protection mediated by periplasmic chaperones in Escherichia coli.

Degree: 2009, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499690

► HdeA and HdeB are stationary phase periplasmic proteins believed to function as acid-induced chaperones to prevent the aggregation of periplasmic proteins at low pH. The… (more)

Subjects/Keywords: 571.29; Escherichia coli

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APA (6^th Edition):

Harding, A. (2009). Mechanisms of acid protection mediated by periplasmic chaperones in Escherichia coli. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499690

Chicago Manual of Style (16^th Edition):

Harding, Amanda. “Mechanisms of acid protection mediated by periplasmic chaperones in Escherichia coli.” 2009. Doctoral Dissertation, University of Aberdeen. Accessed February 22, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499690.

MLA Handbook (7^th Edition):

Harding, Amanda. “Mechanisms of acid protection mediated by periplasmic chaperones in Escherichia coli.” 2009. Web. 22 Feb 2020.

Vancouver:

Harding A. Mechanisms of acid protection mediated by periplasmic chaperones in Escherichia coli. [Internet] [Doctoral dissertation]. University of Aberdeen; 2009. [cited 2020 Feb 22]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499690.

Council of Science Editors:

Harding A. Mechanisms of acid protection mediated by periplasmic chaperones in Escherichia coli. [Doctoral Dissertation]. University of Aberdeen; 2009. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499690

University of Hawaii

8. Geisselsoder, Janet. Correlation between intramolecular base composition heterogeneity of DNA and control of transcriptional expression in E. coli temperate phage P2.

Degree: PhD, 2009, University of Hawaii

URL: http://hdl.handle.net/10125/11481

►

Typescript.

Bibliography: leaves 91-96.

ix, 96 l illus

Intramolecular base compositional heterogeneity has been demonstrated in the DNA of the phage P2 by following the… (more)

▼

Typescript.

Bibliography: leaves 91-96.

ix, 96 l illus

Intramolecular base compositional heterogeneity has been demonstrated in the DNA of the phage P2 by following the optical density of solutions at 260 nm as a function of increasing temperature. First derivative curves of the functions thus generated have been obtained for P2 DNA and the DNA of two closely related phages. Comparison of these curves reveals similarities at the high temperature (high GC) end and differences at the low temperature (low GC) end. All three of these phages can, by supplying the genes concerned with phage particle maturation, serve as helper for the defective phage P4. The strands of P2 DNA have been separated on the basis of their buoyant densities in CsCl solutions when complexed with poly UG. In vivo transcription patterns from these strands in cells infected with various P2 mutants have demonstrated that the "heavy" strand is the one predominantly transcribed, that some "light" strand transcription originates from an operon coding for proteins involved in phage head assembly (thus fuling out a "read-through" mechanism for late gene activation), and that early "light" strand transcription does not originate from DNA deleted in two mutants which is in the right half of the physical map. There is, however, some "light" strand transcription early in infection. P2 DNA has been sheared in: half and the halves separated on the basis of Hg++ binding in Cs2SO4 density gradients. Electron microscopic analysis of partially denatured molecules in these preparations have fixed them with respect to the physical map. In ~ transcription originates primarily from the right, or low GC half early in infection and then shifts to the left, or high GC half. Mutants in genes A and B, which are located in the right half of the genetic map, are defective in both DNA replication and in effecting this shift. Infection with one polar amber mutant under non-permissive conditions has demonstrated that this operon lies :in the right half of the DNA and thus helps to fix the physical map with respect to the genetic map. Two of those regions of the phage DNA which are known to be transcribed from a repressed genome, namely the prophage, appear to be of quite low GC content.

Subjects/Keywords: DNA; Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Geisselsoder, J. (2009). Correlation between intramolecular base composition heterogeneity of DNA and control of transcriptional expression in E. coli temperate phage P2. (Doctoral Dissertation). University of Hawaii. Retrieved from http://hdl.handle.net/10125/11481

Chicago Manual of Style (16^th Edition):

Geisselsoder, Janet. “Correlation between intramolecular base composition heterogeneity of DNA and control of transcriptional expression in E. coli temperate phage P2.” 2009. Doctoral Dissertation, University of Hawaii. Accessed February 22, 2020. http://hdl.handle.net/10125/11481.

MLA Handbook (7^th Edition):

Geisselsoder, Janet. “Correlation between intramolecular base composition heterogeneity of DNA and control of transcriptional expression in E. coli temperate phage P2.” 2009. Web. 22 Feb 2020.

Vancouver:

Geisselsoder J. Correlation between intramolecular base composition heterogeneity of DNA and control of transcriptional expression in E. coli temperate phage P2. [Internet] [Doctoral dissertation]. University of Hawaii; 2009. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/10125/11481.

Council of Science Editors:

Geisselsoder J. Correlation between intramolecular base composition heterogeneity of DNA and control of transcriptional expression in E. coli temperate phage P2. [Doctoral Dissertation]. University of Hawaii; 2009. Available from: http://hdl.handle.net/10125/11481

University of Hong Kong

9. 肖敏鳳; Xiao, Minfeng. The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli.

Degree: PhD, 2013, University of Hong Kong

URL: Xiao, M. [肖敏鳳]. (2013). The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153671 ; http://dx.doi.org/10.5353/th_b5153671 ; http://hdl.handle.net/10722/206531

► ompW encodes a widespread outer-membrane porin protein in Gram-negative bacteria. It has been implicated in bacterial responses to various antibiotics and environmental substances such as… (more)

▼ ompW encodes a widespread outer-membrane porin protein in Gram-negative bacteria. It has been implicated in bacterial responses to various antibiotics and environmental substances such as antibiotics, drugs and mouse mucus etc. Little is known, however, about its regulation and physiological roles during bacterial stress responses. Recently, comparative genomics studies revealed that the ompW gene is a core regulon of the global transcription factor FNR (Fumarate Nitrate Reduction) which mediates the transition from aerobic to anaerobic lifestyle of facultative bacteria. Anaerobiosis represents a predominant challenge encountered by many bacteria in their natural ecological niches and human hosts. This thesis thus aims to elucidate the molecular mechanism of FNR-dependent regulation of ompW expression and its relevance to the anaerobic adaption of the model facultative bacterium E. coli. Regulation of ompW expression by several other key physiological signals related to the anaerobiosis of E. coli, as well as the physiological significance, is also explored systematically. In the first half of the thesis, FNR-dependent regulation of ompW is confirmed by in vivo transcriptional activity assay, and then further confirmed at mRNA and protein level by RT-qPCR and western blotting. EMSA combined with transcriptional activity assay reveals that FNR directly binds with two sites centered at -81.5 and -126.5 bp respectively on ompW promoter (PompW). While binding to the -81.5 site by FNR activates the transcription of ompW, interaction with the -126.5 site represses it, and repression through the -126.5 site is dependent on primary occupancy of the -81.5 site by FNR. Based on these molecular mechanisms, a novel regulatory model of ompW expression during anaerobic adaptation of E. coli is proposed. Growth competition assay further confirmed the physiological significance of this fine-tuned regulation of ompW by FNR in facilitating the fitness and adaptation of E. coli during the transition from aerobic to micro-aerobic and anaerobic lifestyles. In the second half of the thesis, it is demonstrated that two other physiological signals related to the anaerobiosis of E. coli participate in the regulation of ompW, i.e. carbon and electron sources. The molecular mechanisms of how the relevant transcription factors, namely CRP and NarXL, mediate ompW transcription were elucidated: CRP activates the transcription of ompW by binding with the -42.5 site on PompW when glucose is absent; NarL represses the expression of ompW via its binding with the -18.5 site on PompW in the presence of nitrate (the most preferred electron source of E. coli during anaerobic growth). Fumarate is estimated to enter the central channel of OmpW and rescues OmpW-mediated colicin S4 killing of E. coli, suggesting OmpW is a receptor for fumarate and revealing its role in facilitating C4-dicarboxylates utilization. In summary, my study reveals a previously unrecognized, highly co-ordinated and dynamic regulation network for the expression of the…

Subjects/Keywords: Escherichia coli - Genetics

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APA (6^th Edition):

肖敏鳳; Xiao, M. (2013). The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. (Doctoral Dissertation). University of Hong Kong. Retrieved from Xiao, M. [肖敏鳳]. (2013). The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153671 ; http://dx.doi.org/10.5353/th_b5153671 ; http://hdl.handle.net/10722/206531

Chicago Manual of Style (16^th Edition):

肖敏鳳; Xiao, Minfeng. “The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed February 22, 2020. Xiao, M. [肖敏鳳]. (2013). The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153671 ; http://dx.doi.org/10.5353/th_b5153671 ; http://hdl.handle.net/10722/206531.

MLA Handbook (7^th Edition):

肖敏鳳; Xiao, Minfeng. “The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli.” 2013. Web. 22 Feb 2020.

Vancouver:

肖敏鳳; Xiao M. The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2020 Feb 22]. Available from: Xiao, M. [肖敏鳳]. (2013). The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153671 ; http://dx.doi.org/10.5353/th_b5153671 ; http://hdl.handle.net/10722/206531.

Council of Science Editors:

肖敏鳳; Xiao M. The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Xiao, M. [肖敏鳳]. (2013). The regulatory mechanisms and physiological functions of an outer membrane protein opmpW during anaerobic adaptation in Escherichia coli. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5153671 ; http://dx.doi.org/10.5353/th_b5153671 ; http://hdl.handle.net/10722/206531

University of Aberdeen

10. Almeida, Camila de. Modelling of the protection mechanisms against methylgyoxal stress in Escherichia coli : dynamical analysis and experimental validation.

Degree: 2009, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=103942 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521153

► The main MG detoxification pathway in Escherichia coli consists of two enzymes, the glyoxalases I and II, and is dependent on glutathione (GSH). MG readily… (more)

Subjects/Keywords: 571.29; Escherichia coli

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APA (6^th Edition):

Almeida, C. d. (2009). Modelling of the protection mechanisms against methylgyoxal stress in Escherichia coli : dynamical analysis and experimental validation. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=103942 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521153

Chicago Manual of Style (16^th Edition):

Almeida, Camila de. “Modelling of the protection mechanisms against methylgyoxal stress in Escherichia coli : dynamical analysis and experimental validation.” 2009. Doctoral Dissertation, University of Aberdeen. Accessed February 22, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=103942 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521153.

MLA Handbook (7^th Edition):

Almeida, Camila de. “Modelling of the protection mechanisms against methylgyoxal stress in Escherichia coli : dynamical analysis and experimental validation.” 2009. Web. 22 Feb 2020.

Vancouver:

Almeida Cd. Modelling of the protection mechanisms against methylgyoxal stress in Escherichia coli : dynamical analysis and experimental validation. [Internet] [Doctoral dissertation]. University of Aberdeen; 2009. [cited 2020 Feb 22]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=103942 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521153.

Council of Science Editors:

Almeida Cd. Modelling of the protection mechanisms against methylgyoxal stress in Escherichia coli : dynamical analysis and experimental validation. [Doctoral Dissertation]. University of Aberdeen; 2009. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=103942 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521153

University of Aberdeen

11. Ozyamak, Ertan. The analysis of methylglyoxal detoxification and stress responses in Escherichia coli.

Degree: 2009, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=59008 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509209

► Bacteria live in frequently changing environments and have to deal with a multitude of challenges. The chemical challenges to be faced are not only of… (more)

Subjects/Keywords: 571.29; Escherichia coli

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APA (6^th Edition):

Ozyamak, E. (2009). The analysis of methylglyoxal detoxification and stress responses in Escherichia coli. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=59008 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509209

Chicago Manual of Style (16^th Edition):

Ozyamak, Ertan. “The analysis of methylglyoxal detoxification and stress responses in Escherichia coli.” 2009. Doctoral Dissertation, University of Aberdeen. Accessed February 22, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=59008 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509209.

MLA Handbook (7^th Edition):

Ozyamak, Ertan. “The analysis of methylglyoxal detoxification and stress responses in Escherichia coli.” 2009. Web. 22 Feb 2020.

Vancouver:

Ozyamak E. The analysis of methylglyoxal detoxification and stress responses in Escherichia coli. [Internet] [Doctoral dissertation]. University of Aberdeen; 2009. [cited 2020 Feb 22]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=59008 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509209.

Council of Science Editors:

Ozyamak E. The analysis of methylglyoxal detoxification and stress responses in Escherichia coli. [Doctoral Dissertation]. University of Aberdeen; 2009. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=59008 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509209

California State University – East Bay

12. Greer, Jameelah. Construction and Purification of N-terminal His6 YqgF Clone.

Degree: MSin Chemistry, 2015, California State University – East Bay

URL: http://hdl.handle.net/10211.3/137874

► The main goal of the study is to produce high levels of the YqgF protein for use in protein-protein interaction studies with YqgE. The specific… (more)

Subjects/Keywords: Escherichia coli – Genetics

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APA (6^th Edition):

Greer, J. (2015). Construction and Purification of N-terminal His6 YqgF Clone. (Masters Thesis). California State University – East Bay. Retrieved from http://hdl.handle.net/10211.3/137874

Chicago Manual of Style (16^th Edition):

Greer, Jameelah. “Construction and Purification of N-terminal His6 YqgF Clone.” 2015. Masters Thesis, California State University – East Bay. Accessed February 22, 2020. http://hdl.handle.net/10211.3/137874.

MLA Handbook (7^th Edition):

Greer, Jameelah. “Construction and Purification of N-terminal His6 YqgF Clone.” 2015. Web. 22 Feb 2020.

Vancouver:

Greer J. Construction and Purification of N-terminal His6 YqgF Clone. [Internet] [Masters thesis]. California State University – East Bay; 2015. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/10211.3/137874.

Council of Science Editors:

Greer J. Construction and Purification of N-terminal His6 YqgF Clone. [Masters Thesis]. California State University – East Bay; 2015. Available from: http://hdl.handle.net/10211.3/137874

University of Wisconsin – La Cross

13. Rentschler, Anne. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.

Degree: 2010, University of Wisconsin – La Cross

URL: http://digital.library.wisc.edu/1793/49107

► Uropathogenic Escherichia coli (UPEC) cause more than 90% of all human urinary tract infections (UTIs). Type 1 pili on the surface of UPEC allow the… (more)

Subjects/Keywords: Escherichia coli infections

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APA (6^th Edition):

Rentschler, A. (2010). In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. (Thesis). University of Wisconsin – La Cross. Retrieved from http://digital.library.wisc.edu/1793/49107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rentschler, Anne. “In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.” 2010. Thesis, University of Wisconsin – La Cross. Accessed February 22, 2020. http://digital.library.wisc.edu/1793/49107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rentschler, Anne. “In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.” 2010. Web. 22 Feb 2020.

Vancouver:

Rentschler A. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. [Internet] [Thesis]. University of Wisconsin – La Cross; 2010. [cited 2020 Feb 22]. Available from: http://digital.library.wisc.edu/1793/49107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rentschler A. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. [Thesis]. University of Wisconsin – La Cross; 2010. Available from: http://digital.library.wisc.edu/1793/49107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

14. Galbiati Belmonte, Heloisa Filus. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.

Degree: PhD, 2016, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230133 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

► Escherichia coli (E. coli) frequently experiences changes in environmental osmolarity thus requiring homeostatic adjustments for proper cell function. In a hypoosmotic shock the central players… (more)

Subjects/Keywords: 579.3; Escherichia coli

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APA (6^th Edition):

Galbiati Belmonte, H. F. (2016). Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230133 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

Chicago Manual of Style (16^th Edition):

Galbiati Belmonte, Heloisa Filus. “Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.” 2016. Doctoral Dissertation, University of Aberdeen. Accessed February 22, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230133 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591.

MLA Handbook (7^th Edition):

Galbiati Belmonte, Heloisa Filus. “Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.” 2016. Web. 22 Feb 2020.

Vancouver:

Galbiati Belmonte HF. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. [Internet] [Doctoral dissertation]. University of Aberdeen; 2016. [cited 2020 Feb 22]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230133 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591.

Council of Science Editors:

Galbiati Belmonte HF. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. [Doctoral Dissertation]. University of Aberdeen; 2016. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230133 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

University of Melbourne

15. Ingle, Danielle Joy. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.

Degree: 2016, University of Melbourne

URL: http://hdl.handle.net/11343/122899

► Atypical enteropathogenic Escherichia coli (aEPEC) is an important emerging pathogen that is causally associated with diarrhoea in children in both industrialised and developing nations. The… (more)

▼ Atypical enteropathogenic Escherichia coli (aEPEC) is an important emerging pathogen that is causally associated with diarrhoea in children in both industrialised and developing nations. The current definition for aEPEC is the presence of the locus of enterocyte effacement (LEE) pathogenicity island and the absence of genes for bundle forming pili (bfpA) and Shiga toxin (stx). Classical approaches to categorise clinical isolates of aEPEC have shown that they are heterogeneous for sequence type, lipopolysaccharide (O-) and flagella (H-) surface antigens, antimicrobial resistance profiles and virulence genes. Associations of aEPEC with diarrhoea are inconsistent, suggesting that the current definition of aEPEC lacks the ability to distinguish between virulent and less virulent isolates. For this thesis, I used comparative genomic analysis to explore the evolution and population structure of aEPEC. A total of 196 putative aEPEC isolates were collected during the Global Enteric Multicenter Study (GEMS) from seven countries in South Asia and Sub-Saharan Africa. These isolates were subjected to whole genome sequencing and 185 were con rmed in silico as aEPEC. The core aEPEC genomes were rst compared with publicly available genomes with phylogenomic analysis revealing ten globally distributed clonal lineages of aEPEC. Investigation into the evolution of the LEE showed that aEPEC clones were associated with different LEE subtypes. Analysis of genetic variation within the LEE revealed three major lineages, comprised of a total of 30 different subtypes. The three LEE lineages were shown to have different preferred tRNA insertion sites on the chromosome and to be associated with different complements of known non-LEE-encoded effector genes. The genes encoding the type III secretion system (T3SS) were found to limited in their evolution whilst genes encoding immunogenic proteins have accumulated extensive genetic diversity suggesting that they are subject to diversifying selection. This genetic variation was utilised in the development of a LEE typing scheme, the utility of which was demonstrated using isolates from public health investigations. The diversity of O- and H-antigens was explored in the aEPEC collection, and a method as developed to facilitate in silico serotyping from short read data. This approach was validated by serological phenotyping of 197 EPEC isolates, before being used to characterise other clinically relevant isolates. Antimicrobial resistance of GEMS aEPEC isolates was characterised phenotypically and genetically, revealing high levels of resistance across geographical sites and clonal lineages. Regional differences between the GEMS sites, that may be due to differences in local drug use, was determined to be a key driver for the acquisition and retention of gene content conferring antimicrobial resistance. Antimicrobial resistance could be reliably predicted from genome data for the majority of drug classes. In summary, this study examined the evolution of the emerging pathogen, aEPEC…

Subjects/Keywords: Escherichia coli; genomics

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APA (6^th Edition):

Ingle, D. J. (2016). Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/122899

Chicago Manual of Style (16^th Edition):

Ingle, Danielle Joy. “Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.” 2016. Doctoral Dissertation, University of Melbourne. Accessed February 22, 2020. http://hdl.handle.net/11343/122899.

MLA Handbook (7^th Edition):

Ingle, Danielle Joy. “Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.” 2016. Web. 22 Feb 2020.

Vancouver:

Ingle DJ. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. [Internet] [Doctoral dissertation]. University of Melbourne; 2016. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/11343/122899.

Council of Science Editors:

Ingle DJ. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. [Doctoral Dissertation]. University of Melbourne; 2016. Available from: http://hdl.handle.net/11343/122899

University of Illinois – Urbana-Champaign

16. Li, Jinbei Walden. Development of in vivo ppGpp reporters in escherichia coli.

Degree: MS, Bioengineering, 2018, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/102515

► ppGpp is a small molecule that works as a global master regulator of the E. coli physiology, most notably during the stringent response. Systems biology… (more)

Subjects/Keywords: ESCHERICHIA COLI; ppGpp

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APA (6^th Edition):

Li, J. W. (2018). Development of in vivo ppGpp reporters in escherichia coli. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/102515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Jinbei Walden. “Development of in vivo ppGpp reporters in escherichia coli.” 2018. Thesis, University of Illinois – Urbana-Champaign. Accessed February 22, 2020. http://hdl.handle.net/2142/102515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Jinbei Walden. “Development of in vivo ppGpp reporters in escherichia coli.” 2018. Web. 22 Feb 2020.

Vancouver:

Li JW. Development of in vivo ppGpp reporters in escherichia coli. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2018. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/2142/102515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li JW. Development of in vivo ppGpp reporters in escherichia coli. [Thesis]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/102515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad Andrés Bello

17. García Mena, Nicole. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido .

Degree: 2017, Universidad Andrés Bello

URL: http://repositorio.unab.cl/xmlui/handle/ria/6855

► El objetivo del proyecto es mejorar la eficiencia catalítica que posee la enzima Pglucuronidasa de Escherichia coli por codefna-6-glucurónido, a fin de reducir el tiempo… (more)

▼ El objetivo del proyecto es mejorar la eficiencia catalítica que posee la enzima Pglucuronidasa de Escherichia coli por codefna-6-glucurónido, a fin de reducir el tiempo de hidrólisis de éste metabolito en test de doping (actualmente se logra niveles de hidrólisis deseable en 12 horas). Asumiendo que la eficiencia catalítica de la enzima se correlaciona con la energía de unión del ligando en una confonnación catalftica, lo que se busca en esta tesis es generar mutantes que mejoren la afinidad por codeína-6-glucurónido en una confonnación catalltica similar al primer estado de transición del mecanismo catalftico. A modo comparativo, se partió el análisis utilizando también morfina-3-glucurónido, para la cual se conoce que la enzima tiene una eficiencia cataHtica mayor que por codefna-6-glucurónido. En el Protein Data Bank (PDB) se encuentran 6 cristales de la enzima con diversos rangos de resolución y largos de secuencia. Para identificar el confónnero que mejor sirva de templado para nuestros estudios de unión de ligandos en confonnaciones catalíticas, se tomó cada una de las cadenas presentes en los 6 cristales del PDB y se realizó ensayos de acoplamiento molecular ( docking) de cada uno de las cadenas con ligandos conocidos ( codefna-6-glucurónido y morfina- 3-glucurónido) usando el programa Autodock Vina. El procedimiento anterior arrojó la cadena A de la estructura 3LPF como el mejor confónnero a partir del cual se llevó a cabo un proceso in silico de generación de mutaciones simples y múltiples usando Modeller de fonna automatizada. Para seleccionar los residuos del sitio activo que serían mutados se utilizó el mejor modelo de docking de 3LPF cadena A y morfina-3-glucurónido o codefna-6-glucurónido, y se identificó los residuos aminoacfdicos que estaban en proximidad de los ligandos usando VMD. Con la selección de las mejores mutantes simples, se generaron luego mutantes dobles y triples con criterios específicos de selección. Finalmente, evaluamos si las posiciones mutadas que resultan en mejoras en la unión de codefna-6-glucurónido en confonnaciones catalfticas ocurren en sitios con adaptación positiva (aquellos donde las mutaciones no sinónimas priman por sobre las mutaciones sinónimas) o sitios con adaptación negativa (mutaciones sinónimas predominan por sobre las no sinónimas). Existe evidencia en la literatura para otras familias de enzimas que los sitios con adaptación positiva están involucrados en cambios de especificidad de sustrato, por tanto, mutaciones racionales en sitios con adaptación positiva reflejarían de mejor manera la evolución natural que seguiría esta enzima en un organismo confrontado en la naturaleza con el sustrato de nuestro interés. Para cumplir este objetivo, se identificaron miembros caracterizados de la familia Glicosil Hidrolasa 2 a la que pertenece la P-glucuronidasa de Escherichia coli. A partir de estos se generó una red de similitud de secuencias con el fin de identificar otros miembros de la familia a partir de bases de datos públicas de… Advisors/Committee Members: Almonacid, Daniel (advisor).

Subjects/Keywords: Escherichia Coli; Enzimas

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APA (6^th Edition):

García Mena, N. (2017). Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido . (Thesis). Universidad Andrés Bello. Retrieved from http://repositorio.unab.cl/xmlui/handle/ria/6855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

García Mena, Nicole. “Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido .” 2017. Thesis, Universidad Andrés Bello. Accessed February 22, 2020. http://repositorio.unab.cl/xmlui/handle/ria/6855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

García Mena, Nicole. “Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido .” 2017. Web. 22 Feb 2020.

Vancouver:

García Mena N. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido . [Internet] [Thesis]. Universidad Andrés Bello; 2017. [cited 2020 Feb 22]. Available from: http://repositorio.unab.cl/xmlui/handle/ria/6855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

García Mena N. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido . [Thesis]. Universidad Andrés Bello; 2017. Available from: http://repositorio.unab.cl/xmlui/handle/ria/6855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

18. Keklak, Julia T., 1991-. The effect of substrate stiffness on uropathogenic E. coli infection.

Degree: MS, Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

► Both mammalian and bacterial cells respond to environmental cues and then elicit various physiological responses. Mechanical cues, such as surface attachment, have been noted as… (more)

Subjects/Keywords: Escherichia coli infections

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Keklak, Julia T., 1. (2016). The effect of substrate stiffness on uropathogenic E. coli infection. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

Chicago Manual of Style (16^th Edition):

Keklak, Julia T., 1991-. “The effect of substrate stiffness on uropathogenic E. coli infection.” 2016. Masters Thesis, Rutgers University. Accessed February 22, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/49806/.

MLA Handbook (7^th Edition):

Keklak, Julia T., 1991-. “The effect of substrate stiffness on uropathogenic E. coli infection.” 2016. Web. 22 Feb 2020.

Vancouver:

Keklak, Julia T. 1. The effect of substrate stiffness on uropathogenic E. coli infection. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2020 Feb 22]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/.

Council of Science Editors:

Keklak, Julia T. 1. The effect of substrate stiffness on uropathogenic E. coli infection. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

Rutgers University

19. Basu, Debaleena. The role of the A1 subunit in the activity of shiga toxins.

Degree: PhD, Plant Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

►

Shiga toxin (Stx) producing E.coli infections can lead to life-threatening complications, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Stx1 and Stx2 are AB5 toxins… (more)

▼

Shiga toxin (Stx) producing E.coli infections can lead to life-threatening complications, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Stx1 and Stx2 are AB5 toxins consisting of an enzymatically active A subunit and a pentamer of receptor binding B subunits. Stx2-producing E.coli strains are more frequently associated with HUS than Stx1-producing strains. The role of the A subunits in the increased potency of Stx2 has not been fully investigated. This study using purified A1 subunits, provide the first direct evidence that the higher affinity for ribosomes in combination with higher catalytic activity towards the SRL allows Stx2A1 to depurinate ribosomes, inhibit translation and exhibit cytotoxicity at a significantly higher level than Stx1A1 in yeast and human cells. To determine if conserved arginines at the distal face of the active site contribute to the higher affinity of Stx2A1 for the ribosome, Arg172, Arg176 and Arg179 in Stx1A1 and Stx2A1 are mutated. It is seen that Arg172 and Arg176 are more important than Arg179 for depurination activity and toxicity of Stx1A1 and Stx2A1. Mutation of at least two of the three arginines is required to significantly reduce depurination by Stx2A1 in vitro and in cells in yeast and mammalian cells. Conserved arginines at the distal face of the active site are critical for interactions of Stx1A1 and Stx2A1 with the stalk, while a conserved arginine at the active site is critical for non-stalk specific interactions with the ribosome. Mutations at conserved arginines at either site reduced ribosome interactions of Stx1A1 and Stx2A1 similarly, indicating that they do not contribute to the higher affinity of Stx2A1 for the ribosome. Interchanging residues E60 and 61 in Stx1A1 and Y60 and Q61 in Stx2A1 that are located away from the active site and ribosome stalk binding site, resulted in small but significant increase in depurination level of E60Y/E61Q for Stx1A1 and a decrease in depurination level of Y60E/Q61E of Stx2A1 in vivo in yeast. A larger difference may be observed if more than two residues are simultaneously altered to change the electrostatic charge distribution of Stx1A1 and Stx2A1 sufficiently.

Advisors/Committee Members: Tumer, Nilgun (chair), White, James (internal member), Belanger, Faith (internal member), Woychik, Nancy (outside member).

Subjects/Keywords: Escherichia coli; Toxins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Basu, D. (2016). The role of the A1 subunit in the activity of shiga toxins. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

Chicago Manual of Style (16^th Edition):

Basu, Debaleena. “The role of the A1 subunit in the activity of shiga toxins.” 2016. Doctoral Dissertation, Rutgers University. Accessed February 22, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/51187/.

MLA Handbook (7^th Edition):

Basu, Debaleena. “The role of the A1 subunit in the activity of shiga toxins.” 2016. Web. 22 Feb 2020.

Vancouver:

Basu D. The role of the A1 subunit in the activity of shiga toxins. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2020 Feb 22]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/.

Council of Science Editors:

Basu D. The role of the A1 subunit in the activity of shiga toxins. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

Ryerson University

20. Gadishaw-Lue, Crystal. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.

Degree: 2015, Ryerson University

URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347

► Enterohemorrhagic Escherichia coli (EHEC) causes severe food and water-borne illness associated with diarrhea, hemorrhagic colitis (HC), and hemolytic-uremic syndrome (HUS). Previously, we reported that treatment… (more)

Subjects/Keywords: Bile salts; Escherichia coli 0157:H7; Escherichia coli infections; Escherichia coli

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APA (6^th Edition):

Gadishaw-Lue, C. (2015). Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A4347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gadishaw-Lue, Crystal. “Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.” 2015. Thesis, Ryerson University. Accessed February 22, 2020. https://digital.library.ryerson.ca/islandora/object/RULA%3A4347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gadishaw-Lue, Crystal. “Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.” 2015. Web. 22 Feb 2020.

Vancouver:

Gadishaw-Lue C. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. [Internet] [Thesis]. Ryerson University; 2015. [cited 2020 Feb 22]. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gadishaw-Lue C. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. [Thesis]. Ryerson University; 2015. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Silva, Mídana Felismino da. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.

Degree: 2009, Universidade da Beira Interior

URL: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048

► O aumento inabalável da resistência da E. coli aos antibióticos e as diferenças geográficas na sua prevalência levam com que seja imprescindíveis os estudos locais… (more)

▼ O aumento inabalável da resistência da E. coli aos antibióticos e as diferenças geográficas na sua prevalência levam com que seja imprescindíveis os estudos locais de prevalências e das tendências evolutivas das resistências. Objectivo: Analisar a prevalência e as tendências evolutivas da resistência da E. coli no distrito de Castelo Branco de Janeiro de 2006 a Dezembro de 2008 Método: Trata-se de um estudo descritivo com uma abordagem quantitativa. Foram analisadas todos os resultados dos testes de sensibilidade aos antibióticos efectuados nos laboratórios de microbiologia do Centro Hospitalar da Cova da Beira e do Hospital Amato Lusitano, obtidos mediante sistemas automatizados para identificação e antibiograma (VITEK®) durante aquele período. Os isolados foram incluídos com base no perfil de sensibilidade e no tempo de isolamento. Resultados: Foram estudados 1138 isolados no HAL e 1714 no CHCB. Observou-se uma elevada taxa de resistência da E. coli à penicilina (> 40%) em ambos os hospitais durante os três anos em causa. Entre os isolados no CHCB observou-se uma tendência decrescente nas taxas de resistências entre 2007 e 2008, à excepção da taxa de resistência à ciprofloxacina que tende a aumentar. Relativamente a resistência dos isolados no HAL, observou-se uma tendência à estabilizar porém, as taxas de resistência à ampicilina e à gentamicina tendem a aumentar. No CHCB observou-se também uma reemergência dos isolados da E. coli produtores de ESBL. No HAL, apesar de se verificar uma ligeira diminuição na sua taxa de prevalência, esta ainda permanece elevada. Conclusão: O presente trabalho revelou as diferenças nas variações evolutivas das taxas de prevalência da resistência da E. coli entre duas regiões do distrito de Castelo Branco à semelhança das encontradas em outras investigações em diferentes regiões e países. Comprovando a necessidade de desenvolvimento de um compromisso local constante, no combate as resistências bem como na adaptação dos protocolos de tratamentos às realidades locais.

Subjects/Keywords: Escherichia coli - Resistência antibiótica; Escherichia coli - Prevalência; Escherichia coli - Tratamento

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APA (6^th Edition):

Silva, M. F. d. (2009). Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. (Thesis). Universidade da Beira Interior. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Mídana Felismino da. “Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.” 2009. Thesis, Universidade da Beira Interior. Accessed February 22, 2020. http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Mídana Felismino da. “Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.” 2009. Web. 22 Feb 2020.

Vancouver:

Silva MFd. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. [Internet] [Thesis]. Universidade da Beira Interior; 2009. [cited 2020 Feb 22]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva MFd. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. [Thesis]. Universidade da Beira Interior; 2009. Available from: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ryerson University

22. Tollman, Hannah. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.

Degree: 2013, Ryerson University

URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861

► Escherichia coli (EHEC) 0157:H7 is a pathogenic bacterial species that is most commonly linked to severe diarrhea, but is also the leading cause of the… (more)

Subjects/Keywords: Escherichia coli; Escherichia coli infections; Escherichia coli O157:H7; Bacteria – Adhesion; Cell adhesion

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APA (6^th Edition):

Tollman, H. (2013). Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A2861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tollman, Hannah. “Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.” 2013. Thesis, Ryerson University. Accessed February 22, 2020. https://digital.library.ryerson.ca/islandora/object/RULA%3A2861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tollman, Hannah. “Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.” 2013. Web. 22 Feb 2020.

Vancouver:

Tollman H. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. [Internet] [Thesis]. Ryerson University; 2013. [cited 2020 Feb 22]. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tollman H. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. [Thesis]. Ryerson University; 2013. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

23. Verma, Renu, 1987-. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade .

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685

► Resumo: O Brasil é o terceiro maior produtor e primeiro exportador de carne de aves do mundo. A bactéria Escherichia coli patogênica para aves (APEC)… (more)

▼ Resumo: O Brasil é o terceiro maior produtor e primeiro exportador de carne de aves do mundo. A bactéria Escherichia coli patogênica para aves (APEC) é responsável por perdas econômicas significativas nesta multi milionária indústria, causando infecções sistêmicas ou localizadas conhecidas coletivamente como colibacilose. Apesar de sua importância, os mecanismos de virulência destes patótipo não foram, ainda, totalmente elucidados. Portanto, neste trabalho, o nosso objetivo foi estudar genes, que poderiam estar potencialmente envolvidos na patogenicidade de uma linhagem APEC, isolada de uma galinha que apresentava sinais clínicos da síndrome da cabeça inchada (SCI-07; O não tipável: H31). Dois genes yadC e yicS, que estavam sob seleção positiva, foram escolhidos para serem deletados e suas contribuições para vários fenótipos relacionados ao processo de patogênese, avaliadas. Para isso, as linhagens mutantes e complementadas foram comparadas com a linhagem selvagem em vários testes, tais como mortalidade, motilidade, ensaios de sobrevivência de macrófagos, adesão e invasão. A linhagem ?yadC mostrou uma menor capacidade de aderência em células epiteliais humana (HeLa) e reduzida capacidade de invasão de células Hep-2, bem como diminuição da capacidade de sobrevivência em macrófagos de origem aviárias (HD11 células). Esta linhagem mutante também níveis reduzidos de mortalidade e apresentou capacidade reduzida na infecção sistêmica. A linhagem ?yicS apresentou diminuição do nível de invasão em CEC-32 e em Hep-2, motilidade reduzida e uma menor capacidade de formação de biofilme. Este mutante apresentou, também, uma diminuição na mortalidade e uma diminuição do número de bactérias recuperadas no teste de infecção sistêmica. Considerando os resultados obtidos neste trabalho é possível concluir que estes genes contribuem e são importantes para o processo de infecção e patogenicidade da linhagem SCI-07; Abstract: Brazil is the third largest producer and first exporter of poultry meat worldwide. Avian Pathogenic Escherichia coli (APEC) is responsible for significant economic losses in multi-million dollar in the industry, by causing systemic or localized diseases collectively known as colibacillosis. Besides its importance, the virulence mechanisms of these pathotypes have not been fully elucidated yet. Therefore, in this work, our aim was to study genes, which could be potentially involved in the pathogenicity of an APEC strain isolated from a chicken presenting clinical signs of Swollen Head Syndrome (strain SCI-07; nontypeable O: H31). Two genes yadC and yicS, which were demonstrated under positive selection, were chosen to be deleted and their contribution to several phenotypes related to pathogenesis process were further evaluated. For this, the mutated and complemented strains were compared to the wild type strain in several tests, such as mortality, motility, survival in macrophage, adhesion and invasion assays. The mutant yadC showed a decreased adhesion capacity in human epithelial (HeLa cells) and reduced ability in… Advisors/Committee Members: Dias da Silveira, Wanderley, 1956- (advisor), Rojas, Thaís Cabrera Galvão, 1980- (advisor), Sircili, Marcelo Palma (committee member), Ramos, Marcelo de Carvalho (committee member), Cipriano, Matheus Aparecido Pereira (committee member), Paiva, Jacqueline Boldrin de (committee member).

Subjects/Keywords: Escherichia coli patogenica aviaria; Colibacilose; Escherichia coli - Patogenicidade; Deleção de genes; Proteínas de Escherichia coli

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APA (6^th Edition):

Verma, Renu, 1. (2016). Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Verma, Renu, 1987-. “Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade .” 2016. Thesis, Universidade Estadual de Campinas. Accessed February 22, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Verma, Renu, 1987-. “Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade .” 2016. Web. 22 Feb 2020.

Vancouver:

Verma, Renu 1. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade . [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2020 Feb 22]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Verma, Renu 1. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade . [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

24. Sukhija, Karan. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.

Degree: 2011, University of Waterloo

URL: http://hdl.handle.net/10012/5982

► A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-flippase recognition targets (FLP-FRT) recombinations was proposed for genomic engineering of Escherichia coli. For demonstration… (more)

Subjects/Keywords: metabolic engineering; e. coli; escherichia coli; recombineering

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APA (6^th Edition):

Sukhija, K. (2011). Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/5982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sukhija, Karan. “Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.” 2011. Thesis, University of Waterloo. Accessed February 22, 2020. http://hdl.handle.net/10012/5982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sukhija, Karan. “Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.” 2011. Web. 22 Feb 2020.

Vancouver:

Sukhija K. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. [Internet] [Thesis]. University of Waterloo; 2011. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/10012/5982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sukhija K. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. [Thesis]. University of Waterloo; 2011. Available from: http://hdl.handle.net/10012/5982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Saaranluoma, Tiia. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon .

Degree: 2019, Theseus

URL: http://www.theseus.fi/handle/10024/262113

► Polymeraasiketjureaktio (PCR) on paljon käytetty menetelmä, jolla on nykyään monia erilaisia käyttökohteita ja se on keskeinen menetelmä molekyylibiologian laboratoriossa. PCR-reaktiossa hyvinkin pienestä määrästä DNA:ta saadaan… (more)

▼ Polymeraasiketjureaktio (PCR) on paljon käytetty menetelmä, jolla on nykyään monia erilaisia käyttökohteita ja se on keskeinen menetelmä molekyylibiologian laboratoriossa. PCR-reaktiossa hyvinkin pienestä määrästä DNA:ta saadaan monistettua moninkertainen määrä samaa DNA:ta, jolloin sitä voidaan käyttää esimerkiksi diagnostisiin tarkoituksiin. Esimerkiksi mikrobiologian laboratoriossa PCR-tekniikkaa käytetään jo hyvin paljon, ja tekniikan käyttö vain lisääntyy. PCR-tekniikkaa avuksi käyttäen voidaan tunnistaa bakteereja ja monistaa DNA-jaksoja. Kvantitatiivisella polymeraasiketjureaktiolla (qPCR) pystytään mittaamaan monistettavan DNA:n määrää reaaliajassa merkkiaineiden avulla. Menetelmän etuina on sen nopeus eikä se tarvitse agaroosigeeliä tulosten tulkintaan. Esimerkiksi tiettyjen bakteereiden osoituksessa siitä on tullut jo rutiinimenetelmä. Opinnäytetyön tavoitteena on tuottaa työohje E. colin määrityksestä kvantitatiivisella PCR-menetelmällä. Opinnäytetyön tarkoituksena on tehdä StepOnePlus™ Real-Time PCR-analysaattorilla määritys, ja tuottaa prosessista toimiva työohje. Työohje tulee Tampereen ammattikorkeakoulun molekyylibiologian laboratorioon opetuskäyttöön. Opinnäytetyö toteutetaan toiminnallisena opinnäytetyönä. Menetelmä testattiin Tampereen ammattikorkeakoulun molekyylibiologian laboratoriossa, ja opinnäytetyö sisältää tarkempaa tietoa prosessin kulusta ja eri työvaiheista. Prosessi lähtee liikkeelle bakteerin genomin eristyksestä päättyen qPCR-määrityksen tuloksiin. Kirjallinen osio antaa pohjatietoa PCR:n perusperiaatteista ja käyttökohteista. Materiaali on hyväksi tueksi työohjeen käyttäjälle myös laboratorioharjoituksiin. Kirjallinen osio avaa opiskelijalle testauksen eri vaiheita, joita suoritettiin työohjeen toimivuuden takaamiseksi. Kehitysehdotuksena työohjetta tulisi testata vielä opiskelijaryhmällä, ja muokata sitä saadun palautteen mukaan. Menetelmää voisi saada kustannustehokkaammaksi optimoimalla reaktion alukkeiden ja SYBR® Greenin määriä.; Polymerase chain reaction (pcr) is widely used as a diagnostic tool in molecular biology laboratory. PCR is able to amplify very small amounts of specific DNA sequence multiple times. This amplified DNA sequence can be used to detect different kinds of bacterial and viral infections. Quantitative PCR (qPCR) can be used to detect this DNA amplification in real-time using different kind of specific fluorescent dyes or probes. The aim of this study was to create an instruction booklet on the detection of E. coli using qPCR analysis. This was accomplished by doing detection using StepOnePlus™ Real-time analyser and creating an instruction booklet based on that detection. This study was conducted as a practice-based study. Before creation of the instruction booklet, two laboratory tests where performed. In the first one, the qPCR reaction was tested and checked if primers or reaction conditions needed optimising. In the second test genomic DNA of E. coli was extracted and quantified using analysis. An instruction booklet was…

Subjects/Keywords: PCR; qPCR; Escherichia coli; E. coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saaranluoma, T. (2019). Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon . (Thesis). Theseus. Retrieved from http://www.theseus.fi/handle/10024/262113

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Saaranluoma, Tiia. “Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon .” 2019. Thesis, Theseus. Accessed February 22, 2020. http://www.theseus.fi/handle/10024/262113.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Saaranluoma, Tiia. “Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon .” 2019. Web. 22 Feb 2020.

Vancouver:

Saaranluoma T. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon . [Internet] [Thesis]. Theseus; 2019. [cited 2020 Feb 22]. Available from: http://www.theseus.fi/handle/10024/262113.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Saaranluoma T. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon . [Thesis]. Theseus; 2019. Available from: http://www.theseus.fi/handle/10024/262113

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

26. Narwankar, Revati. Effect of point mutations on the toxicity of shiga toxin A1 subunit.

Degree: MS, Food Science, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/

► Shiga toxin (Stx)-producing Escherichia coli (STEC) infections can lead to life- threatening complications, including hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS), which is the most… (more)

Subjects/Keywords: E. coli; Escherichia coli – Toxicology – Genetic aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Narwankar, R. (2019). Effect of point mutations on the toxicity of shiga toxin A1 subunit. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61827/

Chicago Manual of Style (16^th Edition):

Narwankar, Revati. “Effect of point mutations on the toxicity of shiga toxin A1 subunit.” 2019. Masters Thesis, Rutgers University. Accessed February 22, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/61827/.

MLA Handbook (7^th Edition):

Narwankar, Revati. “Effect of point mutations on the toxicity of shiga toxin A1 subunit.” 2019. Web. 22 Feb 2020.

Vancouver:

Narwankar R. Effect of point mutations on the toxicity of shiga toxin A1 subunit. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2020 Feb 22]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/.

Council of Science Editors:

Narwankar R. Effect of point mutations on the toxicity of shiga toxin A1 subunit. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/

Universidade do Rio Grande do Sul

27. Teichmann, Aline. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.

Degree: 2010, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/30207

►

O Echinococcus granulosus é um platelminto parasita da classe Cestoda, que tem, como hospedeiros definitivos, os canídeos, e, como hospedeiros intermediários mais comuns, ungulados domésticos… (more)

▼

O Echinococcus granulosus é um platelminto parasita da classe Cestoda, que tem, como hospedeiros definitivos, os canídeos, e, como hospedeiros intermediários mais comuns, ungulados domésticos e o homem. Este parasito é o agente etiológico da hidatidose cística, zoonose hiperendêmica no Cone Sul da América do Sul, incluindo o sul do Brasil. Para o estabelecimento e manutenção do cisto hidático por longos períodos de tempo (infecção crônica), o parasito secreta e expõe ao seu hospedeiro numerosas proteínas, que podem apresentar tanto atividade imunomoduladora, como estar relacionadas a atividades fisiológicas do parasito. As proteínas 14-3-3 constituem uma família altamente conservada de moléculas reguladoras eucarióticas, que são capazes de interagir com diferentes proteínas ligantes em diversos contextos celulares, regulando funções biológicas complexas. Em E. granulosus, foram identificadas cinco proteínas 14-3-3, sendo três isoformas ζ e duas isoformas ε. Essas proteínas podem estar envolvidas em interações parasito-hospedeiro que propiciam o estabelecimento e o desenvolvimento da forma larval patogênica (metacestódeo). Para a futura caracterização funcional da proteína 14-3-3e1 de E. granulousus (Eg14-3-3ε1), já detectada em componentes do metacestódeo, a sua sequência codificadora foi expressada em Escherichia coli. A clonagem foi realizada em dois vetores plasmidiais distintos (pGEX-TEV e pET-26b), para permitir a expressão em E. coli da proteína Eg14-3-3ε1 recombinante no citoplasma, em fusão com a GST, e no espaço periplásmatico, com cauda de histidina, respectivamente. A solubilização da Eg14-3-3ε1 em fusão com a GST foi obtida com 0,5% de sarcosil, mas no entanto não foi possível a sua purificação. A proteína Eg14-3-3ε1 com cauda de histidina foi obtida por extração da fração periplasmática seguida da purificação por cromatografia de afinidade, tendo sido obtido um rendimento final de 0,5 mg/l de cultura. A proteína Eg14-3-3ε1 recombinante purificada será futuramente utilizada em ensaios funcionais, buscando inicialmente a identificação de seus ligantes proteicos dentre o repertório de proteínas do parasito e do hospedeiro, no contexto da infecção crônica.

Echinococcus granulosus is a parasitc platyhelminth of the Cestoda class, wich has, canids as definitive hosts, domestic ungulates and humans as intermediate hosts. This parasite is the causative agent of cystic hydatid disease, a hyperendemic zoonosis in the Southern Cone of South America, including Southern Brazil. In order to the establishment and maintenance of hydatid cyst for a long period of time, the parasite secrets and exposes to its host numerous proteins, which may have immunomodulatory activity as well to be related to physiological activities of the parasite. The 14-3-3 proteins are a family of highly conserved eukaryotic regulatory molecules that are able to interact with different partner proteins in different cellular contexts, regulating complex biological functions. In E. granulosus five 14-3-3 proteins have been identified, three ζ…

Advisors/Committee Members: Ferreira, Henrique Bunselmeyer.

Subjects/Keywords: Clonagem; Echinococcus granulosus; Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teichmann, A. (2010). Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/30207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Teichmann, Aline. “Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.” 2010. Thesis, Universidade do Rio Grande do Sul. Accessed February 22, 2020. http://hdl.handle.net/10183/30207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Teichmann, Aline. “Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.” 2010. Web. 22 Feb 2020.

Vancouver:

Teichmann A. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2010. [cited 2020 Feb 22]. Available from: http://hdl.handle.net/10183/30207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Teichmann A. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. [Thesis]. Universidade do Rio Grande do Sul; 2010. Available from: http://hdl.handle.net/10183/30207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

28. Ma, Yingjie. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.

Degree: MS, Biological Engineering, 2009, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/ma_yingjie_200908_ms

► The primary goal of this research is to understand the physiological cause for overflow metabolism of E. coli in order to improve the yield and… (more)

Subjects/Keywords: Protein production; Acetate; Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ma, Y. (2009). Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/ma_yingjie_200908_ms

Chicago Manual of Style (16^th Edition):

Ma, Yingjie. “Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.” 2009. Masters Thesis, University of Georgia. Accessed February 22, 2020. http://purl.galileo.usg.edu/uga_etd/ma_yingjie_200908_ms.

MLA Handbook (7^th Edition):

Ma, Yingjie. “Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli.” 2009. Web. 22 Feb 2020.

Vancouver:

Ma Y. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. [Internet] [Masters thesis]. University of Georgia; 2009. [cited 2020 Feb 22]. Available from: http://purl.galileo.usg.edu/uga_etd/ma_yingjie_200908_ms.

Council of Science Editors:

Ma Y. Overcoming acetate in recombinant protein production by metabolic engineering of Escherichia coli. [Masters Thesis]. University of Georgia; 2009. Available from: http://purl.galileo.usg.edu/uga_etd/ma_yingjie_200908_ms

University of Georgia

29. Park, Yoen Ju. Degradation of cellulose and curli, inactivation of Shiga toxin producing Escherichia coli cells, and control of biofilms using treatments with selected enzymes, organic acids or commercial detergents.

Degree: PhD, Food Science, 2010, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/park_yoen-ju_201012_phd

► This study was undertaken to determine the efficacies of different concentrations of cellulase or protease, 2% acetic and lactic acid and a manufacturer-recommended concentration of… (more)

Subjects/Keywords: Shiga toxin producing Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Park, Y. J. (2010). Degradation of cellulose and curli, inactivation of Shiga toxin producing Escherichia coli cells, and control of biofilms using treatments with selected enzymes, organic acids or commercial detergents. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/park_yoen-ju_201012_phd

Chicago Manual of Style (16^th Edition):

Park, Yoen Ju. “Degradation of cellulose and curli, inactivation of Shiga toxin producing Escherichia coli cells, and control of biofilms using treatments with selected enzymes, organic acids or commercial detergents.” 2010. Doctoral Dissertation, University of Georgia. Accessed February 22, 2020. http://purl.galileo.usg.edu/uga_etd/park_yoen-ju_201012_phd.

MLA Handbook (7^th Edition):

Park, Yoen Ju. “Degradation of cellulose and curli, inactivation of Shiga toxin producing Escherichia coli cells, and control of biofilms using treatments with selected enzymes, organic acids or commercial detergents.” 2010. Web. 22 Feb 2020.

Vancouver:

Park YJ. Degradation of cellulose and curli, inactivation of Shiga toxin producing Escherichia coli cells, and control of biofilms using treatments with selected enzymes, organic acids or commercial detergents. [Internet] [Doctoral dissertation]. University of Georgia; 2010. [cited 2020 Feb 22]. Available from: http://purl.galileo.usg.edu/uga_etd/park_yoen-ju_201012_phd.

Council of Science Editors:

Park YJ. Degradation of cellulose and curli, inactivation of Shiga toxin producing Escherichia coli cells, and control of biofilms using treatments with selected enzymes, organic acids or commercial detergents. [Doctoral Dissertation]. University of Georgia; 2010. Available from: http://purl.galileo.usg.edu/uga_etd/park_yoen-ju_201012_phd

University of Georgia

30. Lee, Chi-Ching. Influence of cell envelope components on the attachment of Escherichia coli O157:H7 to lettuce and spinach under different ionic environments: effect on their resistance to environmental stress.

Degree: PhD, Food Science, 2014, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/lee_chi-ching_201405_phd

► Produce-related outbreaks of Escherichia coli O157:H7 are the second most common foodborne outbreaks since it was first recognized an enteric pathogen in 1982. The mechanism… (more)

Subjects/Keywords: Escherichia coli O157:H7

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, C. (2014). Influence of cell envelope components on the attachment of Escherichia coli O157:H7 to lettuce and spinach under different ionic environments: effect on their resistance to environmental stress. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/lee_chi-ching_201405_phd

Chicago Manual of Style (16^th Edition):

Lee, Chi-Ching. “Influence of cell envelope components on the attachment of Escherichia coli O157:H7 to lettuce and spinach under different ionic environments: effect on their resistance to environmental stress.” 2014. Doctoral Dissertation, University of Georgia. Accessed February 22, 2020. http://purl.galileo.usg.edu/uga_etd/lee_chi-ching_201405_phd.

MLA Handbook (7^th Edition):

Lee, Chi-Ching. “Influence of cell envelope components on the attachment of Escherichia coli O157:H7 to lettuce and spinach under different ionic environments: effect on their resistance to environmental stress.” 2014. Web. 22 Feb 2020.

Vancouver:

Lee C. Influence of cell envelope components on the attachment of Escherichia coli O157:H7 to lettuce and spinach under different ionic environments: effect on their resistance to environmental stress. [Internet] [Doctoral dissertation]. University of Georgia; 2014. [cited 2020 Feb 22]. Available from: http://purl.galileo.usg.edu/uga_etd/lee_chi-ching_201405_phd.

Council of Science Editors:

Lee C. Influence of cell envelope components on the attachment of Escherichia coli O157:H7 to lettuce and spinach under different ionic environments: effect on their resistance to environmental stress. [Doctoral Dissertation]. University of Georgia; 2014. Available from: http://purl.galileo.usg.edu/uga_etd/lee_chi-ching_201405_phd

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Open Access Theses and Dissertations