subject:(Escherichia coli)

Universidad de Cantabria

1. Márquez López, Alicia. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.

Degree: 2016, Universidad de Cantabria

► RESUMEN: Caracterización molecular de aislamientos de Escherichia coli procedentes de distintas muestras clínicas para tratar de evaluar la relación entre la resistencia a quinolonas y… (more)

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Márquez López, A. (2016). Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. (Doctoral Dissertation). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/8428

Chicago Manual of Style (16^th Edition):

Márquez López, Alicia. “Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.” 2016. Doctoral Dissertation, Universidad de Cantabria. Accessed February 26, 2021. http://hdl.handle.net/10902/8428.

MLA Handbook (7^th Edition):

Márquez López, Alicia. “Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.” 2016. Web. 26 Feb 2021.

Vancouver:

Márquez López A. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. [Internet] [Doctoral dissertation]. Universidad de Cantabria; 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10902/8428.

Council of Science Editors:

Márquez López A. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. [Doctoral Dissertation]. Universidad de Cantabria; 2016. Available from: http://hdl.handle.net/10902/8428

California State University – East Bay

2. Herbold, Nicole M. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.

Degree: MSin Biological Sciences, 2014, California State University – East Bay

URL: http://hdl.handle.net/10211.3/130388

► There is a continuous need for improved accuracy and rapidity for detection methods applicable to foodborne pathogens; especially non-O157 Escherichia coli. Many E. coli, such… (more)

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Herbold, N. M. (2014). Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. (Masters Thesis). California State University – East Bay. Retrieved from http://hdl.handle.net/10211.3/130388

Chicago Manual of Style (16^th Edition):

Herbold, Nicole M. “Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.” 2014. Masters Thesis, California State University – East Bay. Accessed February 26, 2021. http://hdl.handle.net/10211.3/130388.

MLA Handbook (7^th Edition):

Herbold, Nicole M. “Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.” 2014. Web. 26 Feb 2021.

Vancouver:

Herbold NM. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. [Internet] [Masters thesis]. California State University – East Bay; 2014. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10211.3/130388.

Council of Science Editors:

Herbold NM. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. [Masters Thesis]. California State University – East Bay; 2014. Available from: http://hdl.handle.net/10211.3/130388

Rutgers University

3. Jang, Hye In, 1985-. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.

Degree: PhD, Food Science, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/

►

Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts… (more)

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Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts for pathogens. In order to enhance safety of fresh produce, it is important to understand the interactions between human enteric pathogens and plants. However, little information is available about the behavior of human enteric pathogens on plants, such as mechanisms of survival and persistence. In this study, we investigated survival and persistence of Escherichia coli O157:H7 and O104:H4 strains on Arabidopsis thaliana and romaine lettuce, as well as production of capsular polysaccharide (CPS) and induction of plant defense response. Colonization study with E. coli O157:H7 86-24 wild-type strain and its isogenic mutants of surface polysaccharides showed that colanic acid-deficient and lipopolysaccharide (LPS)-deficient mutants significantly less survived on Arabidopsis plant and lettuce on day 1 and 5 post-inoculation, compared to the wild-type. The two mutants of colanic acid and LPS induced 2-fold greater PR1 gene expression and produced significantly lower amount of CPS compared to wild-type strain (P < 0.05). The results may suggest that structures of colanic acid and LPS of E. coli O157:H7 influence the plant defense response, thereby resulting in different survival and colonization on plants. To investigate fitness of an emerging Shiga toxin-producing E. coli (STEC), colonization of E. coli O104:H4 strains on plants were compared with of that of E. coli O157:H7 strains. Results showed that E. coli O104:H4 strains (RG1, C3493, and LpfA) significantly survived better than E. coli O157:H7 strains (7386 and sakai) on Arabidopsis plant and lettuce at day 5, with greater production of CPS and lower expression of PR1 gene (P < 0.05). These results indicate that different level of plant defense response and CPS production may have an impact on survival or fitness of E. coli O104:H4 and O157:H7 on plants. In order to develop control strategies in crop cultivation environments, it is essential to learn about the behavior of human enteric pathogens on plants, particularly factors influencing the ability of pathogen to overcome plant host immunity. The present study provides better understanding of roles of plant defense response and surface polysaccharides on the molecular interactions between human pathogens and plants. Interestingly, the similar trend of bacterial survival/persistence between Arabidopsis (model plant) and lettuce (plant crop) may suggest a potential use of Arabidopsis as an appropriate model plant for studying the mechanisms of plant responses to human enteric pathogens on leafy vegetables. This study also provides an insight into potential roles of CPS in the survival of human enteric pathogens.

Advisors/Committee Members: Matthews, Karl R (chair), School of Graduate Studies.

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Jang, Hye In, 1. (2017). Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55512/

Chicago Manual of Style (16^th Edition):

Jang, Hye In, 1985-. “Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.” 2017. Doctoral Dissertation, Rutgers University. Accessed February 26, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/55512/.

MLA Handbook (7^th Edition):

Jang, Hye In, 1985-. “Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.” 2017. Web. 26 Feb 2021.

Vancouver:

Jang, Hye In 1. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2021 Feb 26]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/.

Council of Science Editors:

Jang, Hye In 1. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/

4. Renata Katsuko Takayama Kobayashi. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.

Degree: 2006, Universidade Estadual de Londrina

URL: http://189.90.64.145/document/?code=vtls000112627

►

Escherichia coli is an important agent of extra-intestinal infections both in humans and animals alike. In poultry, it is responsible for significant economic loss, being… (more)

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Escherichia coli is an important agent of extra-intestinal infections both in humans and animals alike. In poultry, it is responsible for significant economic loss, being known as the pathogenic avian E. coli (APEC); and it causes colibacillosis, which starts as an infection of the respiratory tract evolving to airsacculitis, pericarditis, perihepatitis and septicemia. Several virulence genes are involved, among which tsh. The tsh gene codifies the autotransporter protein temperature sensitive hemagglutinin (Tsh), which has hemagglutinating and proteolytic activities. Tsh is synthesized as 140 kDa precursor protein, whose processing results in 106 kDa passenger domain (Tshs) and 33 kDa beta-domain (Tshb). In our study, we observed that these proteins presence is dependent on temperature and on the medium utilized for bacterial growth. We also verified that both the recombinant Tsh (140 kDa) and the pellets from wild sample APEC 13, which contain Tshb (33 kDa), agglutinated chicken erythrocytes. Both the recombinant Tsh (140kDa) and the supernatants from APEC 13, which contain Tshs (106 kDa), caused proteolysis of mucin. Chicken anti-Tsh serum inhibited the hemagglutinating activity of strains APEC 13 and recombinant E. coli BL21/pET101-tsh, and it also inhibited the mucinolytic activity of Tsh protein. The proof of Tsh protein mucinolytic activity was achieved by means of SDS-PAGE in gels copolymerized with mucins from different sources.

Escherichia coli é um agente importante de infecções extraintestinais em humanos e em animais. Em aves, é responsável por grandes perdas econômicas, sendo conhecida como E. coli patogênica para aves (APEC), e causa a colibacilose, a qual inicia como infecção do trato respiratório e evolui para aerosaculite, pericardite, perihepatite e septicemia. Vários genes de virulência estão envolvidos, sendo tsh um deles. O gene tsh codifica a proteína autotransportada hemaglutinina temperatura sensível (Tsh), que tem atividade hemaglutinante e proteolítica. Tsh é sintetizada como uma proteína precursora de 140 kDa, que é clivada, em um domínio passageiro de 106 kDa (Tshs) e um beta-domínio de 33 kDa (Tshb). Em nosso trabalho observamos que a presença destas proteínas é dependente da temperatura e do meio de cultura utilizado para o crescimento bacteriano. Observamos ainda que, tanto a Tsh recombinante (140 kDa) quanto o sedimento da amostra selvagem APEC 13, que contém Tshb (33 kDa), aglutinaram eritrócitos de galinha. Tanto a Tsh recombinante (140kDa) quanto o sobrenadante da APEC 13, que contém Tshs (106 kDa), causam proteólise da mucina. O anti-Tsh do soro de galinha inibiu a hemaglutinação da APEC 13 e da recombinante E. coli BL21/pET101-tsh, e inibiu também a atividade mucinolítica da proteína Tsh. A comprovação da atividade mucinolítica da proteína Tsh, foi possível através de SDS-PAGE em géis copolimerizados com mucinas de diferentes origens.

Advisors/Committee Members: Luiz Carlos Jabur Gaziri ., Ermerson José Venancio, Halha Ostrensky Saridakis, Ionice Felipe, Marilda Carlos Vidotto.

Subjects/Keywords: Escherichia coli; Escherichia coli; Microbiologia; Microbiology

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APA (6^th Edition):

Kobayashi, R. K. T. (2006). Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. (Thesis). Universidade Estadual de Londrina. Retrieved from http://189.90.64.145/document/?code=vtls000112627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kobayashi, Renata Katsuko Takayama. “Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.” 2006. Thesis, Universidade Estadual de Londrina. Accessed February 26, 2021. http://189.90.64.145/document/?code=vtls000112627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kobayashi, Renata Katsuko Takayama. “Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.” 2006. Web. 26 Feb 2021.

Vancouver:

Kobayashi RKT. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. [Internet] [Thesis]. Universidade Estadual de Londrina; 2006. [cited 2021 Feb 26]. Available from: http://189.90.64.145/document/?code=vtls000112627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kobayashi RKT. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. [Thesis]. Universidade Estadual de Londrina; 2006. Available from: http://189.90.64.145/document/?code=vtls000112627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – La Cross

5. Rentschler, Anne. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.

Degree: 2010, University of Wisconsin – La Cross

URL: http://digital.library.wisc.edu/1793/49107

► Uropathogenic Escherichia coli (UPEC) cause more than 90% of all human urinary tract infections (UTIs). Type 1 pili on the surface of UPEC allow the… (more)

Subjects/Keywords: Escherichia coli infections

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APA (6^th Edition):

Rentschler, A. (2010). In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. (Thesis). University of Wisconsin – La Cross. Retrieved from http://digital.library.wisc.edu/1793/49107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rentschler, Anne. “In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.” 2010. Thesis, University of Wisconsin – La Cross. Accessed February 26, 2021. http://digital.library.wisc.edu/1793/49107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rentschler, Anne. “In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.” 2010. Web. 26 Feb 2021.

Vancouver:

Rentschler A. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. [Internet] [Thesis]. University of Wisconsin – La Cross; 2010. [cited 2021 Feb 26]. Available from: http://digital.library.wisc.edu/1793/49107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rentschler A. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. [Thesis]. University of Wisconsin – La Cross; 2010. Available from: http://digital.library.wisc.edu/1793/49107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

California State University – East Bay

6. Greer, Jameelah. Construction and Purification of N-terminal His6 YqgF Clone.

Degree: MSin Chemistry, 2015, California State University – East Bay

URL: http://hdl.handle.net/10211.3/137874

► The main goal of the study is to produce high levels of the YqgF protein for use in protein-protein interaction studies with YqgE. The specific… (more)

Subjects/Keywords: Escherichia coli – Genetics

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APA (6^th Edition):

Greer, J. (2015). Construction and Purification of N-terminal His6 YqgF Clone. (Masters Thesis). California State University – East Bay. Retrieved from http://hdl.handle.net/10211.3/137874

Chicago Manual of Style (16^th Edition):

Greer, Jameelah. “Construction and Purification of N-terminal His6 YqgF Clone.” 2015. Masters Thesis, California State University – East Bay. Accessed February 26, 2021. http://hdl.handle.net/10211.3/137874.

MLA Handbook (7^th Edition):

Greer, Jameelah. “Construction and Purification of N-terminal His6 YqgF Clone.” 2015. Web. 26 Feb 2021.

Vancouver:

Greer J. Construction and Purification of N-terminal His6 YqgF Clone. [Internet] [Masters thesis]. California State University – East Bay; 2015. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10211.3/137874.

Council of Science Editors:

Greer J. Construction and Purification of N-terminal His6 YqgF Clone. [Masters Thesis]. California State University – East Bay; 2015. Available from: http://hdl.handle.net/10211.3/137874

University of Illinois – Urbana-Champaign

7. Li, Jinbei Walden. Development of in vivo ppGpp reporters in escherichia coli.

Degree: MS, Bioengineering, 2018, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/102515

► ppGpp is a small molecule that works as a global master regulator of the E. coli physiology, most notably during the stringent response. Systems biology… (more)

Subjects/Keywords: ESCHERICHIA COLI; ppGpp

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APA (6^th Edition):

Li, J. W. (2018). Development of in vivo ppGpp reporters in escherichia coli. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/102515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Jinbei Walden. “Development of in vivo ppGpp reporters in escherichia coli.” 2018. Thesis, University of Illinois – Urbana-Champaign. Accessed February 26, 2021. http://hdl.handle.net/2142/102515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Jinbei Walden. “Development of in vivo ppGpp reporters in escherichia coli.” 2018. Web. 26 Feb 2021.

Vancouver:

Li JW. Development of in vivo ppGpp reporters in escherichia coli. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2142/102515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li JW. Development of in vivo ppGpp reporters in escherichia coli. [Thesis]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/102515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

8. Ingle, Danielle Joy. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.

Degree: 2016, University of Melbourne

URL: http://hdl.handle.net/11343/122899

► Atypical enteropathogenic Escherichia coli (aEPEC) is an important emerging pathogen that is causally associated with diarrhoea in children in both industrialised and developing nations. The… (more)

▼ Atypical enteropathogenic Escherichia coli (aEPEC) is an important emerging pathogen that is causally associated with diarrhoea in children in both industrialised and developing nations. The current definition for aEPEC is the presence of the locus of enterocyte effacement (LEE) pathogenicity island and the absence of genes for bundle forming pili (bfpA) and Shiga toxin (stx). Classical approaches to categorise clinical isolates of aEPEC have shown that they are heterogeneous for sequence type, lipopolysaccharide (O-) and flagella (H-) surface antigens, antimicrobial resistance profiles and virulence genes. Associations of aEPEC with diarrhoea are inconsistent, suggesting that the current definition of aEPEC lacks the ability to distinguish between virulent and less virulent isolates. For this thesis, I used comparative genomic analysis to explore the evolution and population structure of aEPEC. A total of 196 putative aEPEC isolates were collected during the Global Enteric Multicenter Study (GEMS) from seven countries in South Asia and Sub-Saharan Africa. These isolates were subjected to whole genome sequencing and 185 were con rmed in silico as aEPEC. The core aEPEC genomes were rst compared with publicly available genomes with phylogenomic analysis revealing ten globally distributed clonal lineages of aEPEC. Investigation into the evolution of the LEE showed that aEPEC clones were associated with different LEE subtypes. Analysis of genetic variation within the LEE revealed three major lineages, comprised of a total of 30 different subtypes. The three LEE lineages were shown to have different preferred tRNA insertion sites on the chromosome and to be associated with different complements of known non-LEE-encoded effector genes. The genes encoding the type III secretion system (T3SS) were found to limited in their evolution whilst genes encoding immunogenic proteins have accumulated extensive genetic diversity suggesting that they are subject to diversifying selection. This genetic variation was utilised in the development of a LEE typing scheme, the utility of which was demonstrated using isolates from public health investigations. The diversity of O- and H-antigens was explored in the aEPEC collection, and a method as developed to facilitate in silico serotyping from short read data. This approach was validated by serological phenotyping of 197 EPEC isolates, before being used to characterise other clinically relevant isolates. Antimicrobial resistance of GEMS aEPEC isolates was characterised phenotypically and genetically, revealing high levels of resistance across geographical sites and clonal lineages. Regional differences between the GEMS sites, that may be due to differences in local drug use, was determined to be a key driver for the acquisition and retention of gene content conferring antimicrobial resistance. Antimicrobial resistance could be reliably predicted from genome data for the majority of drug classes. In summary, this study examined the evolution of the emerging pathogen, aEPEC…

Subjects/Keywords: Escherichia coli; genomics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ingle, D. J. (2016). Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/122899

Chicago Manual of Style (16^th Edition):

Ingle, Danielle Joy. “Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.” 2016. Doctoral Dissertation, University of Melbourne. Accessed February 26, 2021. http://hdl.handle.net/11343/122899.

MLA Handbook (7^th Edition):

Ingle, Danielle Joy. “Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.” 2016. Web. 26 Feb 2021.

Vancouver:

Ingle DJ. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. [Internet] [Doctoral dissertation]. University of Melbourne; 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/11343/122899.

Council of Science Editors:

Ingle DJ. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. [Doctoral Dissertation]. University of Melbourne; 2016. Available from: http://hdl.handle.net/11343/122899

Universidad Andrés Bello

9. García Mena, Nicole. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido .

Degree: 2017, Universidad Andrés Bello

URL: http://repositorio.unab.cl/xmlui/handle/ria/6855

► El objetivo del proyecto es mejorar la eficiencia catalítica que posee la enzima Pglucuronidasa de Escherichia coli por codefna-6-glucurónido, a fin de reducir el tiempo… (more)

▼ El objetivo del proyecto es mejorar la eficiencia catalítica que posee la enzima Pglucuronidasa de Escherichia coli por codefna-6-glucurónido, a fin de reducir el tiempo de hidrólisis de éste metabolito en test de doping (actualmente se logra niveles de hidrólisis deseable en 12 horas). Asumiendo que la eficiencia catalítica de la enzima se correlaciona con la energía de unión del ligando en una confonnación catalftica, lo que se busca en esta tesis es generar mutantes que mejoren la afinidad por codeína-6-glucurónido en una confonnación catalltica similar al primer estado de transición del mecanismo catalftico. A modo comparativo, se partió el análisis utilizando también morfina-3-glucurónido, para la cual se conoce que la enzima tiene una eficiencia cataHtica mayor que por codefna-6-glucurónido. En el Protein Data Bank (PDB) se encuentran 6 cristales de la enzima con diversos rangos de resolución y largos de secuencia. Para identificar el confónnero que mejor sirva de templado para nuestros estudios de unión de ligandos en confonnaciones catalíticas, se tomó cada una de las cadenas presentes en los 6 cristales del PDB y se realizó ensayos de acoplamiento molecular ( docking) de cada uno de las cadenas con ligandos conocidos ( codefna-6-glucurónido y morfina- 3-glucurónido) usando el programa Autodock Vina. El procedimiento anterior arrojó la cadena A de la estructura 3LPF como el mejor confónnero a partir del cual se llevó a cabo un proceso in silico de generación de mutaciones simples y múltiples usando Modeller de fonna automatizada. Para seleccionar los residuos del sitio activo que serían mutados se utilizó el mejor modelo de docking de 3LPF cadena A y morfina-3-glucurónido o codefna-6-glucurónido, y se identificó los residuos aminoacfdicos que estaban en proximidad de los ligandos usando VMD. Con la selección de las mejores mutantes simples, se generaron luego mutantes dobles y triples con criterios específicos de selección. Finalmente, evaluamos si las posiciones mutadas que resultan en mejoras en la unión de codefna-6-glucurónido en confonnaciones catalfticas ocurren en sitios con adaptación positiva (aquellos donde las mutaciones no sinónimas priman por sobre las mutaciones sinónimas) o sitios con adaptación negativa (mutaciones sinónimas predominan por sobre las no sinónimas). Existe evidencia en la literatura para otras familias de enzimas que los sitios con adaptación positiva están involucrados en cambios de especificidad de sustrato, por tanto, mutaciones racionales en sitios con adaptación positiva reflejarían de mejor manera la evolución natural que seguiría esta enzima en un organismo confrontado en la naturaleza con el sustrato de nuestro interés. Para cumplir este objetivo, se identificaron miembros caracterizados de la familia Glicosil Hidrolasa 2 a la que pertenece la P-glucuronidasa de Escherichia coli. A partir de estos se generó una red de similitud de secuencias con el fin de identificar otros miembros de la familia a partir de bases de datos públicas de… Advisors/Committee Members: Almonacid, Daniel (advisor).

Subjects/Keywords: Escherichia Coli; Enzimas

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APA (6^th Edition):

García Mena, N. (2017). Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido . (Thesis). Universidad Andrés Bello. Retrieved from http://repositorio.unab.cl/xmlui/handle/ria/6855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

García Mena, Nicole. “Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido .” 2017. Thesis, Universidad Andrés Bello. Accessed February 26, 2021. http://repositorio.unab.cl/xmlui/handle/ria/6855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

García Mena, Nicole. “Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido .” 2017. Web. 26 Feb 2021.

Vancouver:

García Mena N. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido . [Internet] [Thesis]. Universidad Andrés Bello; 2017. [cited 2021 Feb 26]. Available from: http://repositorio.unab.cl/xmlui/handle/ria/6855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

García Mena N. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido . [Thesis]. Universidad Andrés Bello; 2017. Available from: http://repositorio.unab.cl/xmlui/handle/ria/6855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

10. Basu, Debaleena. The role of the A1 subunit in the activity of shiga toxins.

Degree: PhD, Plant Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

►

Shiga toxin (Stx) producing E.coli infections can lead to life-threatening complications, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Stx1 and Stx2 are AB5 toxins… (more)

▼

Shiga toxin (Stx) producing E.coli infections can lead to life-threatening complications, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Stx1 and Stx2 are AB5 toxins consisting of an enzymatically active A subunit and a pentamer of receptor binding B subunits. Stx2-producing E.coli strains are more frequently associated with HUS than Stx1-producing strains. The role of the A subunits in the increased potency of Stx2 has not been fully investigated. This study using purified A1 subunits, provide the first direct evidence that the higher affinity for ribosomes in combination with higher catalytic activity towards the SRL allows Stx2A1 to depurinate ribosomes, inhibit translation and exhibit cytotoxicity at a significantly higher level than Stx1A1 in yeast and human cells. To determine if conserved arginines at the distal face of the active site contribute to the higher affinity of Stx2A1 for the ribosome, Arg172, Arg176 and Arg179 in Stx1A1 and Stx2A1 are mutated. It is seen that Arg172 and Arg176 are more important than Arg179 for depurination activity and toxicity of Stx1A1 and Stx2A1. Mutation of at least two of the three arginines is required to significantly reduce depurination by Stx2A1 in vitro and in cells in yeast and mammalian cells. Conserved arginines at the distal face of the active site are critical for interactions of Stx1A1 and Stx2A1 with the stalk, while a conserved arginine at the active site is critical for non-stalk specific interactions with the ribosome. Mutations at conserved arginines at either site reduced ribosome interactions of Stx1A1 and Stx2A1 similarly, indicating that they do not contribute to the higher affinity of Stx2A1 for the ribosome. Interchanging residues E60 and 61 in Stx1A1 and Y60 and Q61 in Stx2A1 that are located away from the active site and ribosome stalk binding site, resulted in small but significant increase in depurination level of E60Y/E61Q for Stx1A1 and a decrease in depurination level of Y60E/Q61E of Stx2A1 in vivo in yeast. A larger difference may be observed if more than two residues are simultaneously altered to change the electrostatic charge distribution of Stx1A1 and Stx2A1 sufficiently.

Advisors/Committee Members: Tumer, Nilgun (chair), White, James (internal member), Belanger, Faith (internal member), Woychik, Nancy (outside member).

Subjects/Keywords: Escherichia coli; Toxins

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APA (6^th Edition):

Basu, D. (2016). The role of the A1 subunit in the activity of shiga toxins. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

Chicago Manual of Style (16^th Edition):

Basu, Debaleena. “The role of the A1 subunit in the activity of shiga toxins.” 2016. Doctoral Dissertation, Rutgers University. Accessed February 26, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51187/.

MLA Handbook (7^th Edition):

Basu, Debaleena. “The role of the A1 subunit in the activity of shiga toxins.” 2016. Web. 26 Feb 2021.

Vancouver:

Basu D. The role of the A1 subunit in the activity of shiga toxins. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Feb 26]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/.

Council of Science Editors:

Basu D. The role of the A1 subunit in the activity of shiga toxins. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

University of Aberdeen

11. Galbiati Belmonte, Heloisa Filus. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.

Degree: PhD, 2016, University of Aberdeen

URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

► Escherichia coli (E. coli) frequently experiences changes in environmental osmolarity thus requiring homeostatic adjustments for proper cell function. In a hypoosmotic shock the central players… (more)

Subjects/Keywords: 579.3; Escherichia coli

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APA (6^th Edition):

Galbiati Belmonte, H. F. (2016). Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

Chicago Manual of Style (16^th Edition):

Galbiati Belmonte, Heloisa Filus. “Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.” 2016. Doctoral Dissertation, University of Aberdeen. Accessed February 26, 2021. https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591.

MLA Handbook (7^th Edition):

Galbiati Belmonte, Heloisa Filus. “Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.” 2016. Web. 26 Feb 2021.

Vancouver:

Galbiati Belmonte HF. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. [Internet] [Doctoral dissertation]. University of Aberdeen; 2016. [cited 2021 Feb 26]. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591.

Council of Science Editors:

Galbiati Belmonte HF. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. [Doctoral Dissertation]. University of Aberdeen; 2016. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

Rutgers University

12. Keklak, Julia T., 1991-. The effect of substrate stiffness on uropathogenic E. coli infection.

Degree: MS, Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

► Both mammalian and bacterial cells respond to environmental cues and then elicit various physiological responses. Mechanical cues, such as surface attachment, have been noted as… (more)

Subjects/Keywords: Escherichia coli infections

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APA (6^th Edition):

Keklak, Julia T., 1. (2016). The effect of substrate stiffness on uropathogenic E. coli infection. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

Chicago Manual of Style (16^th Edition):

Keklak, Julia T., 1991-. “The effect of substrate stiffness on uropathogenic E. coli infection.” 2016. Masters Thesis, Rutgers University. Accessed February 26, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49806/.

MLA Handbook (7^th Edition):

Keklak, Julia T., 1991-. “The effect of substrate stiffness on uropathogenic E. coli infection.” 2016. Web. 26 Feb 2021.

Vancouver:

Keklak, Julia T. 1. The effect of substrate stiffness on uropathogenic E. coli infection. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Feb 26]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/.

Council of Science Editors:

Keklak, Julia T. 1. The effect of substrate stiffness on uropathogenic E. coli infection. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

Ryerson University

13. Gadishaw-Lue, Crystal. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.

Degree: 2015, Ryerson University

URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347

► Enterohemorrhagic Escherichia coli (EHEC) causes severe food and water-borne illness associated with diarrhea, hemorrhagic colitis (HC), and hemolytic-uremic syndrome (HUS). Previously, we reported that treatment… (more)

Subjects/Keywords: Bile salts; Escherichia coli 0157:H7; Escherichia coli infections; Escherichia coli

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APA (6^th Edition):

Gadishaw-Lue, C. (2015). Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A4347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gadishaw-Lue, Crystal. “Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.” 2015. Thesis, Ryerson University. Accessed February 26, 2021. https://digital.library.ryerson.ca/islandora/object/RULA%3A4347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gadishaw-Lue, Crystal. “Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.” 2015. Web. 26 Feb 2021.

Vancouver:

Gadishaw-Lue C. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. [Internet] [Thesis]. Ryerson University; 2015. [cited 2021 Feb 26]. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gadishaw-Lue C. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. [Thesis]. Ryerson University; 2015. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Silva, Mídana Felismino da. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.

Degree: 2009, Universidade da Beira Interior

URL: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048

► O aumento inabalável da resistência da E. coli aos antibióticos e as diferenças geográficas na sua prevalência levam com que seja imprescindíveis os estudos locais… (more)

▼ O aumento inabalável da resistência da E. coli aos antibióticos e as diferenças geográficas na sua prevalência levam com que seja imprescindíveis os estudos locais de prevalências e das tendências evolutivas das resistências. Objectivo: Analisar a prevalência e as tendências evolutivas da resistência da E. coli no distrito de Castelo Branco de Janeiro de 2006 a Dezembro de 2008 Método: Trata-se de um estudo descritivo com uma abordagem quantitativa. Foram analisadas todos os resultados dos testes de sensibilidade aos antibióticos efectuados nos laboratórios de microbiologia do Centro Hospitalar da Cova da Beira e do Hospital Amato Lusitano, obtidos mediante sistemas automatizados para identificação e antibiograma (VITEK®) durante aquele período. Os isolados foram incluídos com base no perfil de sensibilidade e no tempo de isolamento. Resultados: Foram estudados 1138 isolados no HAL e 1714 no CHCB. Observou-se uma elevada taxa de resistência da E. coli à penicilina (> 40%) em ambos os hospitais durante os três anos em causa. Entre os isolados no CHCB observou-se uma tendência decrescente nas taxas de resistências entre 2007 e 2008, à excepção da taxa de resistência à ciprofloxacina que tende a aumentar. Relativamente a resistência dos isolados no HAL, observou-se uma tendência à estabilizar porém, as taxas de resistência à ampicilina e à gentamicina tendem a aumentar. No CHCB observou-se também uma reemergência dos isolados da E. coli produtores de ESBL. No HAL, apesar de se verificar uma ligeira diminuição na sua taxa de prevalência, esta ainda permanece elevada. Conclusão: O presente trabalho revelou as diferenças nas variações evolutivas das taxas de prevalência da resistência da E. coli entre duas regiões do distrito de Castelo Branco à semelhança das encontradas em outras investigações em diferentes regiões e países. Comprovando a necessidade de desenvolvimento de um compromisso local constante, no combate as resistências bem como na adaptação dos protocolos de tratamentos às realidades locais.

Subjects/Keywords: Escherichia coli - Resistência antibiótica; Escherichia coli - Prevalência; Escherichia coli - Tratamento

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APA (6^th Edition):

Silva, M. F. d. (2009). Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. (Thesis). Universidade da Beira Interior. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silva, Mídana Felismino da. “Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.” 2009. Thesis, Universidade da Beira Interior. Accessed February 26, 2021. http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silva, Mídana Felismino da. “Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.” 2009. Web. 26 Feb 2021.

Vancouver:

Silva MFd. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. [Internet] [Thesis]. Universidade da Beira Interior; 2009. [cited 2021 Feb 26]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva MFd. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. [Thesis]. Universidade da Beira Interior; 2009. Available from: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

15. Verma, Renu, 1987-. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685

► Abstract: Brazil is the third largest producer and first exporter of poultry meat worldwide. Avian Pathogenic Escherichia coli (APEC) is responsible for significant economic losses… (more)

▼ Abstract: Brazil is the third largest producer and first exporter of poultry meat worldwide. Avian Pathogenic Escherichia coli (APEC) is responsible for significant economic losses in multi-million dollar in the industry, by causing systemic or localized diseases collectively known as colibacillosis. Besides its importance, the virulence mechanisms of these pathotypes have not been fully elucidated yet. Therefore, in this work, our aim was to study genes, which could be potentially involved in the pathogenicity of an APEC strain isolated from a chicken presenting clinical signs of Swollen Head Syndrome (strain SCI-07; nontypeable O: H31). Two genes yadC and yicS, which were demonstrated under positive selection, were chosen to be deleted and their contribution to several phenotypes related to pathogenesis process were further evaluated. For this, the mutated and complemented strains were compared to the wild type strain in several tests, such as mortality, motility, survival in macrophage, adhesion and invasion assays. The mutant yadC showed a decreased adhesion capacity in human epithelial (HeLa cells) and reduced ability in invasion to epithelial cells (Hep-2 cells) as well as decreased capacity of survival in avian macrophages (HD11 cells). The mutant also reduced mortality levels, as well as a reduced ability in the systemic animal infection experiment. The mutant yicS showed a decreased invasion level in CEC-32 cells and Hep-2 cell, reduced motility and a lower capacity of biofilm formation. This mutant also presented a decrease in mortality in one-day old-chicks model and systemic infection experiments. Taken together, these results indicate that these genes contribute to the infectious process and are important for the pathogenicity of strain SCI-07 Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Dias da Silveira, Wanderley, 1956- (advisor), Rojas, Thaís Cabrera Galvão, 1980- (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Sircili, Marcelo Palma (committee member), Ramos, Marcelo de Carvalho (committee member), Cipriano, Matheus Aparecido Pereira (committee member), Paiva, Jacqueline Boldrin de (committee member).

Subjects/Keywords: Escherichia coli patogenica aviaria; Colibacilose; Escherichia coli - Patogenicidade; Deleção de genes; Proteínas de Escherichia coli; Avian pathogenic Escherichia coli; Escherichia coli infections; Escherichia coli - Pathogenicity; Gene deletion; Escherichia coli proteins

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APA (6^th Edition):

Verma, Renu, 1. (2016). Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Verma, Renu, 1987-. “Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade.” 2016. Thesis, Universidade Estadual de Campinas. Accessed February 26, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Verma, Renu, 1987-. “Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade.” 2016. Web. 26 Feb 2021.

Vancouver:

Verma, Renu 1. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Feb 26]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Verma, Renu 1. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ryerson University

16. Tollman, Hannah. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.

Degree: 2013, Ryerson University

URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861

► Escherichia coli (EHEC) 0157:H7 is a pathogenic bacterial species that is most commonly linked to severe diarrhea, but is also the leading cause of the… (more)

Subjects/Keywords: Escherichia coli; Escherichia coli infections; Escherichia coli O157:H7; Bacteria – Adhesion; Cell adhesion

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APA (6^th Edition):

Tollman, H. (2013). Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A2861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tollman, Hannah. “Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.” 2013. Thesis, Ryerson University. Accessed February 26, 2021. https://digital.library.ryerson.ca/islandora/object/RULA%3A2861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tollman, Hannah. “Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.” 2013. Web. 26 Feb 2021.

Vancouver:

Tollman H. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. [Internet] [Thesis]. Ryerson University; 2013. [cited 2021 Feb 26]. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tollman H. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. [Thesis]. Ryerson University; 2013. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Laine, Matti. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon.

Degree: 2019, Theseus

URL: http://www.theseus.fi/handle/10024/262113

►

Polymeraasiketjureaktio (PCR) on paljon käytetty menetelmä, jolla on nykyään monia erilaisia käyttökohteita ja se on keskeinen menetelmä molekyylibiologian laboratoriossa. PCR-reaktiossa hyvinkin pienestä määrästä DNA:ta saadaan… (more)

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Polymeraasiketjureaktio (PCR) on paljon käytetty menetelmä, jolla on nykyään monia erilaisia käyttökohteita ja se on keskeinen menetelmä molekyylibiologian laboratoriossa. PCR-reaktiossa hyvinkin pienestä määrästä DNA:ta saadaan monistettua moninkertainen määrä samaa DNA:ta, jolloin sitä voidaan käyttää esimerkiksi diagnostisiin tarkoituksiin. Esimerkiksi mikrobiologian laboratoriossa PCR-tekniikkaa käytetään jo hyvin paljon, ja tekniikan käyttö vain lisääntyy. PCR-tekniikkaa avuksi käyttäen voidaan tunnistaa bakteereja ja monistaa DNA-jaksoja. Kvantitatiivisella polymeraasiketjureaktiolla (qPCR) pystytään mittaamaan monistettavan DNA:n määrää reaaliajassa merkkiaineiden avulla. Menetelmän etuina on sen nopeus eikä se tarvitse agaroosigeeliä tulosten tulkintaan. Esimerkiksi tiettyjen bakteereiden osoituksessa siitä on tullut jo rutiinimenetelmä. Opinnäytetyön tavoitteena on tuottaa työohje E. colin määrityksestä kvantitatiivisella PCR-menetelmällä. Opinnäytetyön tarkoituksena on tehdä StepOnePlus™ Real-Time PCR-analysaattorilla määritys, ja tuottaa prosessista toimiva työohje. Työohje tulee Tampereen ammattikorkeakoulun molekyylibiologian laboratorioon opetuskäyttöön. Opinnäytetyö toteutetaan toiminnallisena opinnäytetyönä. Menetelmä testattiin Tampereen ammattikorkeakoulun molekyylibiologian laboratoriossa, ja opinnäytetyö sisältää tarkempaa tietoa prosessin kulusta ja eri työvaiheista. Prosessi lähtee liikkeelle bakteerin genomin eristyksestä päättyen qPCR-määrityksen tuloksiin. Kirjallinen osio antaa pohjatietoa PCR:n perusperiaatteista ja käyttökohteista. Materiaali on hyväksi tueksi työohjeen käyttäjälle myös laboratorioharjoituksiin. Kirjallinen osio avaa opiskelijalle testauksen eri vaiheita, joita suoritettiin työohjeen toimivuuden takaamiseksi. Kehitysehdotuksena työohjetta tulisi testata vielä opiskelijaryhmällä, ja muokata sitä saadun palautteen mukaan. Menetelmää voisi saada kustannustehokkaammaksi optimoimalla reaktion alukkeiden ja SYBR® Greenin määriä.

Polymerase chain reaction (pcr) is widely used as a diagnostic tool in molecular biology laboratory. PCR is able to amplify very small amounts of specific DNA sequence multiple times. This amplified DNA sequence can be used to detect different kinds of bacterial and viral infections. Quantitative PCR (qPCR) can be used to detect this DNA amplification in real-time using different kind of specific fluorescent dyes or probes. The aim of this study was to create an instruction booklet on the detection of E. coli using qPCR analysis. This was accomplished by doing detection using StepOnePlus™ Real-time analyser and creating an instruction booklet based on that detection. This study was conducted as a practice-based study. Before creation of the instruction booklet, two laboratory tests where performed. In the first one, the qPCR reaction was tested and checked if primers or reaction conditions needed optimising. In the second test genomic DNA of E. coli was extracted and quantified using analysis. An instruction booklet was…

Subjects/Keywords: PCR; qPCR; Escherichia coli; E. coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Laine, M. (2019). Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon. (Thesis). Theseus. Retrieved from http://www.theseus.fi/handle/10024/262113

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Laine, Matti. “Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon.” 2019. Thesis, Theseus. Accessed February 26, 2021. http://www.theseus.fi/handle/10024/262113.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Laine, Matti. “Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon.” 2019. Web. 26 Feb 2021.

Vancouver:

Laine M. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon. [Internet] [Thesis]. Theseus; 2019. [cited 2021 Feb 26]. Available from: http://www.theseus.fi/handle/10024/262113.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Laine M. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon. [Thesis]. Theseus; 2019. Available from: http://www.theseus.fi/handle/10024/262113

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – Oshkosh

18. Fossen, Jenna L. A Genomic View of Escherichia Coli Diversity from Dairy Calves.

Degree: 2019, University of Wisconsin – Oshkosh

URL: http://digital.library.wisc.edu/1793/79925

► Escherichia coli is a model organism for the scientific community but there are still numerous gaps in our knowledge, including its diversity within the gastrointestinal… (more)

Subjects/Keywords: Escherichia coli; E. coli; Dairy Calves; Genes

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APA (6^th Edition):

Fossen, J. L. (2019). A Genomic View of Escherichia Coli Diversity from Dairy Calves. (Thesis). University of Wisconsin – Oshkosh. Retrieved from http://digital.library.wisc.edu/1793/79925

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fossen, Jenna L. “A Genomic View of Escherichia Coli Diversity from Dairy Calves.” 2019. Thesis, University of Wisconsin – Oshkosh. Accessed February 26, 2021. http://digital.library.wisc.edu/1793/79925.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fossen, Jenna L. “A Genomic View of Escherichia Coli Diversity from Dairy Calves.” 2019. Web. 26 Feb 2021.

Vancouver:

Fossen JL. A Genomic View of Escherichia Coli Diversity from Dairy Calves. [Internet] [Thesis]. University of Wisconsin – Oshkosh; 2019. [cited 2021 Feb 26]. Available from: http://digital.library.wisc.edu/1793/79925.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fossen JL. A Genomic View of Escherichia Coli Diversity from Dairy Calves. [Thesis]. University of Wisconsin – Oshkosh; 2019. Available from: http://digital.library.wisc.edu/1793/79925

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

19. Sukhija, Karan. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.

Degree: 2011, University of Waterloo

URL: http://hdl.handle.net/10012/5982

► A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-flippase recognition targets (FLP-FRT) recombinations was proposed for genomic engineering of Escherichia coli. For demonstration… (more)

Subjects/Keywords: metabolic engineering; e. coli; escherichia coli; recombineering

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APA (6^th Edition):

Sukhija, K. (2011). Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/5982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sukhija, Karan. “Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.” 2011. Thesis, University of Waterloo. Accessed February 26, 2021. http://hdl.handle.net/10012/5982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sukhija, Karan. “Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.” 2011. Web. 26 Feb 2021.

Vancouver:

Sukhija K. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. [Internet] [Thesis]. University of Waterloo; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10012/5982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sukhija K. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. [Thesis]. University of Waterloo; 2011. Available from: http://hdl.handle.net/10012/5982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

20. Narwankar, Revati. Effect of point mutations on the toxicity of shiga toxin A1 subunit.

Degree: MS, Food Science, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/

► Shiga toxin (Stx)-producing Escherichia coli (STEC) infections can lead to life- threatening complications, including hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS), which is the most… (more)

Subjects/Keywords: E. coli; Escherichia coli – Toxicology – Genetic aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Narwankar, R. (2019). Effect of point mutations on the toxicity of shiga toxin A1 subunit. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61827/

Chicago Manual of Style (16^th Edition):

Narwankar, Revati. “Effect of point mutations on the toxicity of shiga toxin A1 subunit.” 2019. Masters Thesis, Rutgers University. Accessed February 26, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/61827/.

MLA Handbook (7^th Edition):

Narwankar, Revati. “Effect of point mutations on the toxicity of shiga toxin A1 subunit.” 2019. Web. 26 Feb 2021.

Vancouver:

Narwankar R. Effect of point mutations on the toxicity of shiga toxin A1 subunit. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Feb 26]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/.

Council of Science Editors:

Narwankar R. Effect of point mutations on the toxicity of shiga toxin A1 subunit. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/

21. Magalhães, Pedro José Santana Ribeiro. Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli.

Degree: 2016, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046

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Dissertação de Mestrado em Biotecnologia para as Ciências da Saúde

A resistência a antibióticos é um problema gobal na medicina humana e veterinária. Escherichia coli… (more)

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Dissertação de Mestrado em Biotecnologia para as Ciências da Saúde

A resistência a antibióticos é um problema gobal na medicina humana e veterinária. Escherichia coli é um microrganismo comensal do trato gastrointestinal de animais e humanos. Esta pode ser utilizada em diversos estudos biológicos e genéticos sobre a resistência aos antibióticos, um problema emergente de saúde pública. A presença do plasmídeo pMdT1 é de grande relevância no estudo da resistência aos antibióticos, visto que contém um gene que codifica uma variante da proteína AAC(6’)-lb-cr e que esta, quando ativa, confere resistência à tobramicina e à canamicina, e diminui a susceptibilidade à ciprofloxacina e à norfloxacina. Foram utilizadas duas amostras, Escherichia coli Electromax DH10B, recetora do processo de transformação e Escherichia coli TF-Se20 sendo esta transformada, pois contém o plasmídeo pMdT1 que expressa o gene da acetilase. Após a extração proteica, a separação e quantificação das proteínas foi realizada mediante a eletroforese monodimensional e posteriormente bidimensional, segundo os princípios de O’Farrel, contudo usando a tecnologia de IPG. As amostras foram sujeitas a uma focalização isoelétrica, seguida de SDS-PAGE. A identificação das proteínas foi realizada através da técnica MALDI-TOF/MS. Os picos de massa obtidos pelo motor de busca Mascot foram comparados com a base de dados Uniprot e NCBI. Para determinar a sequência da proteína de interesse, utilizou-se a técnica LC-MS/MS. Da amostra Escherichia coli TF-Se20 foram excisados 260 spots. Destes, foram identificados 111, e foi possível identificar 76 proteínas distintas, 71 das quais com função conhecida. Foram excisados 225 spots da amostra de Escherichia coli Electromax DH10B, tendo sido identificados 119, dos quais foi possível identificar 72 proteínas diferentes (71 têm processo biológico). As proteínas identificadas participam em diversos processos biológicos como no processo da glicólise, no processo de oxidação-redução e biossíntese de proteínas. A proteína de interesse, aminoglicosidase N(6')-acetiltransferase tipo 1, foi identificada e posteriormente sequenciada. Com um sinal correto, os resultados foram analisados através do Mascot e do Peaks, permitindo a identificação da proteína AAC com seis péptidos. Foi possível a identificação do péptido YSIVTNS(T) DSVTLR, que apresenta uma mutação de substituição, Asn (Aspargina) por uma Thr (Treonina). Para o péptido SHIVEWWGGEEARPTLAD VQE, apenas se obteve o match no Mascot. Como não se confirmou o match no Peaks, a classificação desta identificação foi “possível mas incerta”. Por sua vez, o péptido IA MLNGEPIGYA QSYVALGSGDGR foi confirmado, devido a ter sido validado várias vezes, quer na sua totalidade, quer de uma só parte. Os péptidos GLGTKLVR, MSNAKTKLGITK e QAFERTRSDA não foram detetados ou sequenciados corretamente. O péptido CYEKAGFE (G) QGTVTTPYG PAVYMVQTR foi validado pelo Mascot, apresentando uma mutação na qual uma Gly (Glicina) substitui uma Arg (Arginina). Concluiu-se desta forma que a proteómica…

Advisors/Committee Members: Igrejas, Gilberto, Santos, Hugo.

Subjects/Keywords: Escherichia coli; Resistência a antibióticos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Magalhães, P. J. S. R. (2016). Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Magalhães, Pedro José Santana Ribeiro. “Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli.” 2016. Thesis, RCAAP. Accessed February 26, 2021. http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Magalhães, Pedro José Santana Ribeiro. “Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli.” 2016. Web. 26 Feb 2021.

Vancouver:

Magalhães PJSR. Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli. [Internet] [Thesis]. RCAAP; 2016. [cited 2021 Feb 26]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Magalhães PJSR. Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli. [Thesis]. RCAAP; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

22. Vargas, Lúcia Rosane Bertholdo. Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros.

Degree: 2008, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/15488

► As plantas possuem um arsenal de substâncias utilizadas como defesa contra patógenos e predadores. A possibilidade de utilizar tais substâncias como biopesticidas revolucionou o estudo… (more)

▼ As plantas possuem um arsenal de substâncias utilizadas como defesa contra patógenos e predadores. A possibilidade de utilizar tais substâncias como biopesticidas revolucionou o estudo das proteínas tóxicas. Proteínas inativadoras de ribossomos (RIPs) e as ureases estão entre as proteínas que são abundantes em plantas. RIPs tipo 2 como a ricina, são muito tóxicas, e podem despurinar ribossomos de várias espécies e induzir lesão em DNA, levando à interrupção da síntese protéica e morte de células. Menos tóxicas que a ricina, a maior parte das RIPs conhecidas são do tipo 1 com apenas uma cadeia polipeptídica de 25 - 32 kDa. As ureases (EC 3.5.1.5) são metaloenzimas dependentes de níquel, que catalisam a hidrólise da uréia para formar amônia e dióxido de carbono. A semente do feijão-de-porco, Canavalia ensiformis, é fonte rica de isoformas de urease, entre elas, a canatoxina (CNTX). A proteína CNTX apresenta atividade inseticida contra diferentes espécies de insetos, e sua toxicidade depende da liberação de um peptídeo interno de 10kDa (pepcanatox), que ocorre por ação das catepsinas do sistema digestivo dos insetos suscetíveis. Um peptídeo equivalente ao pepcanatox foi obtido por expressão heteróloga em Escherichia coli - Jaburetox-2Ec, o qual apresentou atividade tóxica contra Dysdercus peruvianus, Rhodnius prolixus e Blatella germanica. Nesse trabalho demonstramos a atividade inseticida de cinco RIPs tipo 1 (PAP-S, gelonina, momordina, saporina-S6 e licnina) em Spodoptera frugiperda e Anticarsia gemmatalis. As RIPs mostraram um efeito entomotóxico espécie-específico para as lagartas, sendo que momordina foi a menos tóxica nos bioensaios. Perda de peso mais pronunciada foi observada em S. frugiperda no 4° dia após o início dos ensaios e para a A. gemmatalis, no 10° dia. A indução da mortalidade (larval e/ou pupal) foi de 57,13% para os tratamentos em A. gemmatalis e 29,45% para S. frugiperda. Para investigar o efeito deterrente de RIPs tipo 1 em insetos, verificou-se, através do teste cometa, o nível de danos ao DNA em tecidos de S. frugiperda e A. gemmatalis que ingeriram um total de 40 μg de RIPs. Os insetos tratados com RIPs mostraram um valor 2 a 3 vezes maior de células com sinais de dano de DNA do que o controle. O dano de DNA poderia ser conseqüência do estresse oxidativo, assim analisou-se atividade de enzimas antioxidantes CAT e SOD e níveis de peroxidação lipídica (TBARS) nos extratos celulares dos insetos, mas não houve uma correlação entre dano de DNA e marcadores de estresse oxidativo. O peptídeo recombinante derivado de urease, jaburetox-2Ec, induziu uma mortalidade de 100% de S. frugiperda após ingestão de 47μg do peptídeo. Em contraste com os dados obtidos com as RIPs, o jaburetox-2Ec não causou lesões no DNA ou alterações em marcadores do balanço redox em S. frugiperda evidenciando um mecanismo de ação distinto. Em linhagens de células de insetos em cultura (Tn5B e UFL-AG-286), a análise citomorfológica sugeriu a ocorrência de citotoxicidade e lise celular com exposição a 80 e 10 μg do… Advisors/Committee Members: Carlini, Celia Regina Ribeiro da Silva.

Subjects/Keywords: Canatoxina; Escherichia coli; Biologia molecular

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vargas, L. R. B. (2008). Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/15488

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vargas, Lúcia Rosane Bertholdo. “Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros.” 2008. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021. http://hdl.handle.net/10183/15488.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vargas, Lúcia Rosane Bertholdo. “Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros.” 2008. Web. 26 Feb 2021.

Vancouver:

Vargas LRB. Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2008. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10183/15488.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vargas LRB. Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros. [Thesis]. Universidade do Rio Grande do Sul; 2008. Available from: http://hdl.handle.net/10183/15488

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

23. Ayres, Raquel M. Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli.

Degree: 2014, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/129475

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As células-tronco pluripotentes induzidas (iPSCs) são as células reprogramadas com fatores de transcrição, como Oct-4, Sox-2, Klf-4 e c-MYC (OSKM). As iPSCs são geralmente obtidas… (more)

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As células-tronco pluripotentes induzidas (iPSCs) são as células reprogramadas com fatores de transcrição, como Oct-4, Sox-2, Klf-4 e c-MYC (OSKM). As iPSCs são geralmente obtidas pela expressão ectópica de vetores retrovirais e lentivirais contendo os fatores transcricionais OSKM. No entanto, a utilização de sistemas de expressão virais pode levar a mutagênese de inserção e geração de células tumorais. Assim, é preferível que a reprogramação celular ocorra temporariamente por meio de fatores OSKM fusionados com sequências de peptideos para a transdução de proteínas. Neste sentido, os genes sintéticos codificadores de transdução de péptidos fusionados ao N ou C-terminal de OSKM pode ser obtido por ferramentas da bioinformática específicos e sintetizados utilizando técnicas químicas avançadas. Esses genes sintéticos podem ser aplicados para a síntese de proteínas utilizando tanto técnicas in vivo quanto in vitro. Assim, o objetivo deste trabalho foi gerar fatores OSKM fusionados a transportana (TP10) usando genes sintéticos e sistema de transcrição e de tradução in vivo. Além disso, três genes sintéticos adicionais também foram obtidos para expressão de Oct-4 contendo TP10 fusionado na região N-terminal (N-OCT4-TP10), C-terminal (C-OCT4-TP10), e sem TP10 (Oct-4). A expressão ectópica dos três genes sintéticos para Oct-4 foram testadas in vivo em duas diferentes linhagens comerciais de E. coli que expressam a enzima de T7 RNA polimerase. Os dados de Western blot indicaram que todos os genes sintéticos de Oct-4 foram capazes de produzir proteínas recombinantes. No entanto, a expressão mais elevada foi obtida com a construção N-OCT4-TP10. Além disso, as proteínas do fator de transcrição Oct-4 obtitdas foram avaliadas quanto a sua capacidade de ligação a sua respectiva seqüência-alvo de DNA sendo avaliada por meio dos extratos brutos das proteínas de E. coli contendo ou não Oct-4 proteínas ectópica. Os dados do ensaio de ligação demonstraram que todas as sequências de Oct-4 foram capazes de se ligar às suas sequências de reconhecimento. Em conclusão, as proteínas Oct-4 funcionais fusionadas com o peptideo o transdutor pode ser obtido a partir de genes sintéticos, permitindo potencialmente elaborar protocolos de reprogramação virais livres.

Induced pluripotent stem cells (iPSCs) are reprogrammed cells with a transcription factors such as Oct-4, Sox-2, Klf-4, and c-MYC (OSKM). iPSCs are generally obtained by ectopic expression of retroviral and lentiviral vectors containing tOSKM. However, the use of viral expression systems can lead to insertional mutagenesis and generation of tumor cells. Thus, it is preferable to temporarily reprogram cell by means of OSKM factors fused to peptides sequences for protein transduction. In this sense, synthetic genes coding to transduction peptides fused to the N- or C-termini of OSKM can be designed by specific bioinformatics tools and synthesized using advanced chemical techniques. Those synthetic genes can be applied for protein synthesis using both in vivo and in vitro techniques.…

Advisors/Committee Members: Bonatto, Diego.

Subjects/Keywords: Escherichia coli; Células-tronco

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ayres, R. M. (2014). Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/129475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ayres, Raquel M. “Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021. http://hdl.handle.net/10183/129475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ayres, Raquel M. “Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli.” 2014. Web. 26 Feb 2021.

Vancouver:

Ayres RM. Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10183/129475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ayres RM. Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/129475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

24. Fernandes, Gabriela de Carvalho. Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação.

Degree: 2014, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/129476

►

O nitrogênio é um elemento essencial à vida na Terra. Em geral, a disponibilização desse elemento para os seres vivos se dá por meio da… (more)

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O nitrogênio é um elemento essencial à vida na Terra. Em geral, a disponibilização desse elemento para os seres vivos se dá por meio da fixação biológica do nitrogênio (FBN). Os micro-organismos capazes de realizar a FBN são denominados de diazotróficos e contêm o complexo enzimático da nitrogenase. Por ser um processo extremamente dispendioso, a FBN é regulada, principalmente, em nível transcricional, em resposta à quantidade de nitrogênio fixado e aos níveis de oxigênio. Os mecanismos de regulação do processo em bactérias Gram-negativas estão bem caracterizados, porém, em bactérias Gram-positivas, os estudos ainda são escassos. Paenibacillus riograndensis é uma bactéria Gram-positiva diazotrófica aeróbia facultativa e formadora de esporos, cujo sequenciamento completo do genoma a capacita como um interessante modelo para o estudo da regulação da FBN. No genoma de P. riograndensis foram identificados três agrupamentos contendo genes relacionados à FBN. Um deles, com uma organização estrutural menos conservada, foi considerado inativo a partir de análises de PCR em tempo real e de atividade de promotor. Os outros dois tiveram seus transcritos identificados e induzidos sob condições de fixação de nitrogênio, sendo um deles responsável pela codificação de um sistema alternativo da nitrogenase, independente de molibdênio. Esse sistema alternativo foi identificado como sendo aquele composto apenas por ferro e validado tanto pela análise das sequências dos genes estruturais, como pela atividade enzimática em meio sem molibdênio. Sequências localizadas a aproximadamente 250 pares de bases (pb) a montante do início da tradução dos primeiros genes dos dois agrupamentos funcionais também tiveram suas atividades como regiões reguladoras validadas pelo reconhecimento em Escherichia coli, com um provável padrão de iniciação da transcrição constitutivo. Uma menor atividade de transcrição foi observada no fragmento de 500 pb localizado a montante do agrupamento dos genes da nitrogenase alternativa, indicando a presença de regiões contendo motivos de regulação negativa do processo. Investigações mais detalhadas dessas sequências podem revelar padrões inéditos para a regulação da FBN em bactérias Gram-positivas, em geral, e em P. riograndensis, em particular.

Nitrogen is an essential element for life. In general, it becomes available to biosphere mainly through biological nitrogen fixation (BNF). Microorganisms named diazotrophs perform BNF and they have the nitrogenase enzyme. As BNF is a very energetic expensive process, it is tightly regulated mainly at transcriptional level in response to available nitrogen and oxygen levels. Regulatory networks comprising BNF systems in Gramnegative bacteria are well characterized, while studies related to Gram-positive bacteria are scarce. Paenibacillus riograndensis is a Gram-positive endospore-forming facultative anaerobic diazotroph, whose complete genome sequence presents it as an interesting model for the study of BNF regulation. In P. riograndensis genome three cluster comprising BNF…

Advisors/Committee Members: Passaglia, Luciane Maria Pereira.

Subjects/Keywords: Paenibacillus; Nitrogen : Fixacao; Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fernandes, G. d. C. (2014). Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/129476

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernandes, Gabriela de Carvalho. “Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021. http://hdl.handle.net/10183/129476.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernandes, Gabriela de Carvalho. “Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação.” 2014. Web. 26 Feb 2021.

Vancouver:

Fernandes GdC. Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10183/129476.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernandes GdC. Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/129476

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

25. Teichmann, Aline. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.

Degree: 2010, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/30207

►

O Echinococcus granulosus é um platelminto parasita da classe Cestoda, que tem, como hospedeiros definitivos, os canídeos, e, como hospedeiros intermediários mais comuns, ungulados domésticos… (more)

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O Echinococcus granulosus é um platelminto parasita da classe Cestoda, que tem, como hospedeiros definitivos, os canídeos, e, como hospedeiros intermediários mais comuns, ungulados domésticos e o homem. Este parasito é o agente etiológico da hidatidose cística, zoonose hiperendêmica no Cone Sul da América do Sul, incluindo o sul do Brasil. Para o estabelecimento e manutenção do cisto hidático por longos períodos de tempo (infecção crônica), o parasito secreta e expõe ao seu hospedeiro numerosas proteínas, que podem apresentar tanto atividade imunomoduladora, como estar relacionadas a atividades fisiológicas do parasito. As proteínas 14-3-3 constituem uma família altamente conservada de moléculas reguladoras eucarióticas, que são capazes de interagir com diferentes proteínas ligantes em diversos contextos celulares, regulando funções biológicas complexas. Em E. granulosus, foram identificadas cinco proteínas 14-3-3, sendo três isoformas ζ e duas isoformas ε. Essas proteínas podem estar envolvidas em interações parasito-hospedeiro que propiciam o estabelecimento e o desenvolvimento da forma larval patogênica (metacestódeo). Para a futura caracterização funcional da proteína 14-3-3e1 de E. granulousus (Eg14-3-3ε1), já detectada em componentes do metacestódeo, a sua sequência codificadora foi expressada em Escherichia coli. A clonagem foi realizada em dois vetores plasmidiais distintos (pGEX-TEV e pET-26b), para permitir a expressão em E. coli da proteína Eg14-3-3ε1 recombinante no citoplasma, em fusão com a GST, e no espaço periplásmatico, com cauda de histidina, respectivamente. A solubilização da Eg14-3-3ε1 em fusão com a GST foi obtida com 0,5% de sarcosil, mas no entanto não foi possível a sua purificação. A proteína Eg14-3-3ε1 com cauda de histidina foi obtida por extração da fração periplasmática seguida da purificação por cromatografia de afinidade, tendo sido obtido um rendimento final de 0,5 mg/l de cultura. A proteína Eg14-3-3ε1 recombinante purificada será futuramente utilizada em ensaios funcionais, buscando inicialmente a identificação de seus ligantes proteicos dentre o repertório de proteínas do parasito e do hospedeiro, no contexto da infecção crônica.

Echinococcus granulosus is a parasitc platyhelminth of the Cestoda class, wich has, canids as definitive hosts, domestic ungulates and humans as intermediate hosts. This parasite is the causative agent of cystic hydatid disease, a hyperendemic zoonosis in the Southern Cone of South America, including Southern Brazil. In order to the establishment and maintenance of hydatid cyst for a long period of time, the parasite secrets and exposes to its host numerous proteins, which may have immunomodulatory activity as well to be related to physiological activities of the parasite. The 14-3-3 proteins are a family of highly conserved eukaryotic regulatory molecules that are able to interact with different partner proteins in different cellular contexts, regulating complex biological functions. In E. granulosus five 14-3-3 proteins have been identified, three ζ…

Advisors/Committee Members: Ferreira, Henrique Bunselmeyer.

Subjects/Keywords: Clonagem; Echinococcus granulosus; Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teichmann, A. (2010). Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/30207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Teichmann, Aline. “Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.” 2010. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021. http://hdl.handle.net/10183/30207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Teichmann, Aline. “Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.” 2010. Web. 26 Feb 2021.

Vancouver:

Teichmann A. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2010. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10183/30207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Teichmann A. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. [Thesis]. Universidade do Rio Grande do Sul; 2010. Available from: http://hdl.handle.net/10183/30207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Casella, Tiago [UNESP]. Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango.

Degree: 2016, Universidade Estadual Paulista

URL: http://hdl.handle.net/11449/144719

► A resistência a cefalosporinas de espectro estendido (ESC) mediada pela produção de β-lactamases de espectro estendido (ESBL) por Enterobacteriaceae é um problema de saúde pública… (more)

▼ A resistência a cefalosporinas de espectro estendido (ESC) mediada pela produção de β-lactamases de espectro estendido (ESBL) por Enterobacteriaceae é um problema de saúde pública global. Neste contexto, o aumento da resistência a ESC entre Escherichia coli isoladas de animais de produção é uma questão importante, uma vez que bactérias comensais de animais de produção podem contaminar os produtos alimentícios e chegar ao intestino humano via cadeia alimentar. O Brasil é um importante produtor e o maior exportador de carne de frango no mundo. Assim, é de extrema importância monitorar a presença dessas bactérias neste setor alimentar. No presente estudo, foi realizada uma comparação do genótipo de resistência a ESC em E. coli isoladas a partir de carnes de frango no Brasil e na França, considerando que não há relação comercial de carne de frango entre esses dois países. Esta abordagem pode ser útil para compreender as dinâmicas dos fatores de resistência na cadeia de produção de frangos. Além disso, linhagens isoladas do trato gastrointestinal de frangos no Brasil foram também estudadas. Para isso, amostras de carnes de frango de diferentes pontos do varejo e swabs de cloaca de frangos de três diferentes fazendas foram coletadas no Estado de São Paulo, Brasil. Também, amostras de carnes de frango de diferentes pontos do varejo de Lyon, França, foram amostradas. As amostras de carne e os swabs de cloaca de frangos foram inoculados em ágar MacConkey acrescido com ESC. As colônias foram identificadas por sistema automatizado, e a suscetibilidade aos antimicrobianos foi testada por disco-difusão. PCR para detecção de genes blaESBL e blapAmpC e para a determinação dos grupos filogenéticos de E. coli foram realizadas. Os genes bla foram sequenciados e os plasmídeos foram caracterizados pelo esquema PBRT e por southern blot/hibridação de géis de PFGE-S1. Quase todas as carnes de frango apresentaram, ao menos, uma linhagem de E. coli resistente a ESC, e 53,8% dos frangos analisados também estavam colonizados por E. coli resistente a ESC. Resistência a aminoglicosídeos, fenicóis, quinolonas, sulfonamidas, tetraciclina e trimetoprim também foi detectada. Um total de 60 linhagens isoladas de amostras do Brasil foram estudadas, e o gene blaCTX-M-2 foi o principal responsável pela resistência a ESC, mas blaCTX-M-55, blaCMY-2, blaCTX-M-15 e blaCTX-M-8 também foram detectados. De 77 linhagens isoladas de amostras de carne de frango da França, o gene blaCTX-M-1 foi o principal responsável pela resistência a ESC, mas blaTEM-52, blaCMY-2 e blaSHV-12 também foram detectados. A maioria dos blaCTX-M-2 estão associados à ISCR1 e a integrons complexos de classe 1, e blaCTX-M-1, blaCTX-M-15, blaCTX-M-55, e os oito blaCMY-2 do Brasil estão precedidos pela ISEcp1. O gene blaCTX-M-8 está associado à IS10. Nas amostras do Brasil, um gene blaCTX-M-2 é carreado por um plasmídeo IncHI2/P, blaCTX-M-8 por um plasmídeo IncI1, um gene blaCTX-M-15 por um plasmídeo IncX1, e os genes blaCTX-M-55 por plasmídeos IncFII ou IncN/FII. Nas amostras de carnes de… Advisors/Committee Members: Nogueira, Mara Corrêa Lelles [UNESP], Gutkind, Gabriel Osvaldo, Madec, Jean-Yves, Universidade Estadual Paulista (UNESP).

Subjects/Keywords: Escherichia coli; ESBL; pAmpC; Frango

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Casella, T. [. (2016). Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/144719

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Casella, Tiago [UNESP]. “Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango.” 2016. Thesis, Universidade Estadual Paulista. Accessed February 26, 2021. http://hdl.handle.net/11449/144719.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Casella, Tiago [UNESP]. “Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango.” 2016. Web. 26 Feb 2021.

Vancouver:

Casella T[. Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango. [Internet] [Thesis]. Universidade Estadual Paulista; 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/11449/144719.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Casella T[. Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango. [Thesis]. Universidade Estadual Paulista; 2016. Available from: http://hdl.handle.net/11449/144719

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

27. Wilson, Jessica Grissett. Investigation of the beneficial effect of Enterobacter cloacae strain JD6301 on mice challenged with Escherichia coli O157:H7.

Degree: MS, Biological Sciences, 2014, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;

► The environment of the gastrointestinal (GI) tract is an extremely complex system made up of not only host cells, but also many beneficial microbes.… (more)

Subjects/Keywords: Escherichia coli; probiotic; Enterobacter cloacae

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APA (6^th Edition):

Wilson, J. G. (2014). Investigation of the beneficial effect of Enterobacter cloacae strain JD6301 on mice challenged with Escherichia coli O157:H7. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;

Chicago Manual of Style (16^th Edition):

Wilson, Jessica Grissett. “Investigation of the beneficial effect of Enterobacter cloacae strain JD6301 on mice challenged with Escherichia coli O157:H7.” 2014. Masters Thesis, Mississippi State University. Accessed February 26, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;.

MLA Handbook (7^th Edition):

Wilson, Jessica Grissett. “Investigation of the beneficial effect of Enterobacter cloacae strain JD6301 on mice challenged with Escherichia coli O157:H7.” 2014. Web. 26 Feb 2021.

Vancouver:

Wilson JG. Investigation of the beneficial effect of Enterobacter cloacae strain JD6301 on mice challenged with Escherichia coli O157:H7. [Internet] [Masters thesis]. Mississippi State University; 2014. [cited 2021 Feb 26]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;.

Council of Science Editors:

Wilson JG. Investigation of the beneficial effect of Enterobacter cloacae strain JD6301 on mice challenged with Escherichia coli O157:H7. [Masters Thesis]. Mississippi State University; 2014. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;

University of Alberta

28. Laidlaw, Anna M. Control and Detection of Enterohaemorrhagic Escherichia coli.

Degree: PhD, Department of Agricultural, Food, and Nutritional Science, 2016, University of Alberta

URL: https://era.library.ualberta.ca/files/c9z902z880

► Enterohaemorrhagic Escherichia coli (EHEC) is a pathogen that causes severe disease in humans and has a low infectious dose. Since foodborne EHEC outbreaks continue to… (more)

▼ Enterohaemorrhagic Escherichia coli (EHEC) is a pathogen that causes severe disease in humans and has a low infectious dose. Since foodborne EHEC outbreaks continue to be a problem worldwide, improved control and detection methods for EHEC on at-risk foods, such as spinach and beef, are essential. While new methods of control and detection may prove to be effective in broth or buffer, it is important that they are also tested in a food matrix since food systems are more complex and may lead to different results. A novel intervention method was developed to control EHEC on spinach and lettuce with a volatile antimicrobial from mustard, allyl isothiocyanate (AITC). AITC released from its precursor sinigrin in mustard meal was limited by the activity of mustard’s endogenous enzyme myrosinase at 4°C. While the requirement of endogenous myrosinase in mustard meal to catalyze this reaction was known, decreased activity at refrigeration temperature was not, and made this antimicrobial intervention on produce impractical. Secondly, this work explored improving EHEC detection methods. Enrichment remains a necessary but lengthy step in pathogen detection methods and a major impediment to rapid detection. Therefore, decreases in the current validated enrichment times for EHEC detection were investigated with aim to reduce pathogen detection times. Individual lag phase of heat injured E. coli O157:H7 cells were measured to determine necessary enrichment times. However, decreasing enrichment times increased the probability of not detecting sub-lethally injured cells since sub-lethal cell injury significantly increased lag phases and therefore overall time to detection. Lastly, a major challenge of qPCR detection is the inability to discriminate between live and dead cells within a sample and this limitation could lead to false positive result. This is especially of importance when using this method for pathogen detection in food. The current work investigated five different detection methods to determine the concentration of viable EHEC cells on beef steaks after interventions of lactic acid, peroxyacetic acid and hot water and included 1) use of a DNA binding dye propidium monoazide (PMA) to prevent dead cell DNA amplification when used in conjunction with qPCR 2) PMA in addition to membrane emulsifying deoxycholate treatment to increase penetration of the dye and therefore increase accuracy of viable cell DNA amplification with qPCR quantification 3) mRNA and 4) rRNA qPCR quantification and 5) conventional plating. Treatment of samples with PMA and deoxycholate was reported in the literature to be successful in broth in preventing dead cell amplification in qPCR and within this research, the same treatment used within a food system confirmed these findings; however, it proved to be more complicated than in broth. While the combination treatment of PMA and deoxycholate prevented amplification of all DNA from dead cells, it also killed the sub-lethally injured cell population within samples and subsequently this rendered their…

Subjects/Keywords: Enterohaemorrhagic Escherichia coli; Detection; Control

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Laidlaw, A. M. (2016). Control and Detection of Enterohaemorrhagic Escherichia coli. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/c9z902z880

Chicago Manual of Style (16^th Edition):

Laidlaw, Anna M. “Control and Detection of Enterohaemorrhagic Escherichia coli.” 2016. Doctoral Dissertation, University of Alberta. Accessed February 26, 2021. https://era.library.ualberta.ca/files/c9z902z880.

MLA Handbook (7^th Edition):

Laidlaw, Anna M. “Control and Detection of Enterohaemorrhagic Escherichia coli.” 2016. Web. 26 Feb 2021.

Vancouver:

Laidlaw AM. Control and Detection of Enterohaemorrhagic Escherichia coli. [Internet] [Doctoral dissertation]. University of Alberta; 2016. [cited 2021 Feb 26]. Available from: https://era.library.ualberta.ca/files/c9z902z880.

Council of Science Editors:

Laidlaw AM. Control and Detection of Enterohaemorrhagic Escherichia coli. [Doctoral Dissertation]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/c9z902z880

Oregon State University

29. Couch, David Bruce. The effect of streptomycin on proline synthesis in Escherichia coli.

Degree: MS, Biochemistry and Biophysics, 1967, Oregon State University

URL: http://hdl.handle.net/1957/47094

► The effect of streptomycin on an early reaction of proline synthesis, the production of glutamic-γ-semialdehyde, in resting cell suspensions of antibiotic-sensitive, resistant, and dependent strains… (more)

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Couch, D. B. (1967). The effect of streptomycin on proline synthesis in Escherichia coli. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47094

Chicago Manual of Style (16^th Edition):

Couch, David Bruce. “The effect of streptomycin on proline synthesis in Escherichia coli.” 1967. Masters Thesis, Oregon State University. Accessed February 26, 2021. http://hdl.handle.net/1957/47094.

MLA Handbook (7^th Edition):

Couch, David Bruce. “The effect of streptomycin on proline synthesis in Escherichia coli.” 1967. Web. 26 Feb 2021.

Vancouver:

Couch DB. The effect of streptomycin on proline synthesis in Escherichia coli. [Internet] [Masters thesis]. Oregon State University; 1967. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1957/47094.

Council of Science Editors:

Couch DB. The effect of streptomycin on proline synthesis in Escherichia coli. [Masters Thesis]. Oregon State University; 1967. Available from: http://hdl.handle.net/1957/47094

Oregon State University

30. Moreno, Daniel. Migration of E. coli and solutes to tile drains via preferential and matrix flow.

Degree: MS, Soil Science, 2002, Oregon State University

URL: http://hdl.handle.net/1957/29676

► The extent of agricultural drainage has created concern for its potential undesirable effects on surface water quality. Land applications of liquid manure on tile drain… (more)

▼ The extent of agricultural drainage has created concern for its potential undesirable effects on surface water quality. Land applications of liquid manure on tile drain fields have the potential to transport solutes and bacteria to the drains following precipitation or irrigation events and many times are directly sent to a surface water body, and have been documented as a source of contamination of surface waters. This study determined the potential for and magnitude of E. coli and solute migration to tile drains through the soil profile. Water from subsurface drains was analyzed for chemical and bacterial composition following tracer applications. Two sites were selected for the study to determine transport at large (field) and small (plot) scales. At the large-scale site, both tracers, bacteria (E. coli and Total Coliform) and Amino-G (a conservative tracer), were used to monitor the speed of transport from the surface to the tile drain following liquid manure applications, tracer applications and additionally precipitation events. The concentrations of E. coli were monitored every hour for 76 days during the spring. Both tracers, bacteria and Amino-G, were detected in the tile drainage shortly after precipitation events. The peak concentration of E. coli was observed to be 1.2 x 10⁶ CFU/l00mL. These elevated concentrations of E. coli might be attributed to the characteristics of the soil, high organic matter and well-structured clay soils. Both the rapid breakthrough of tracer to the tile drain and the peaks of tile water temperature during precipitation events provided evidence of macropore flow. Antecedent soil moisture and warmer temperatures appeared to provide ideal conditions for bacteria growth. The small-scale study site was selected for a more focused study. The purpose of this site was to quantify more accurately the percent mass of surface applied tracer that was transported to the tile drain, allowing mass balance calculations. Experiments were conducted during the summer to control the rate and total amount of irrigation. Amino-G readings were taken every 10 seconds for 125 hours of continuous irrigation. Tracer applications were conducted at runoff and non-runoff conditions. Both types of tracer applications had Amino-G breakthrough in less than 10 minutes after initiation of irrigation. Tracer applied at runoff rates resulted in 4 to 17 times more total tracer mass migrating to the tile drain than when applied at non-runoff rates. The total mass of Amino-G migrating to the tile drain during non-runoff conditions depended on the total volume of applied tracer, regardless of the tracer concentration. For an application of 5.6 mm at 12 mg/L, 5.7% of the total applied tracer migrated to the tile drain, whereas for an application of 1.9 mm at 27.7 mg/L only 2.8% of the total applied tracer migrated to the tile drain. Tile flow response to irrigation experiments appeared to be governed by soil moisture. Lysimeter samples were taken continuously every 4-8 hours until… Advisors/Committee Members: Dragila, Maria I. (advisor), Buckhouse, John (committee member).

Subjects/Keywords: Escherichia coli

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APA (6^th Edition):

Moreno, D. (2002). Migration of E. coli and solutes to tile drains via preferential and matrix flow. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/29676

Chicago Manual of Style (16^th Edition):

Moreno, Daniel. “Migration of E. coli and solutes to tile drains via preferential and matrix flow.” 2002. Masters Thesis, Oregon State University. Accessed February 26, 2021. http://hdl.handle.net/1957/29676.

MLA Handbook (7^th Edition):

Moreno, Daniel. “Migration of E. coli and solutes to tile drains via preferential and matrix flow.” 2002. Web. 26 Feb 2021.

Vancouver:

Moreno D. Migration of E. coli and solutes to tile drains via preferential and matrix flow. [Internet] [Masters thesis]. Oregon State University; 2002. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1957/29676.

Council of Science Editors:

Moreno D. Migration of E. coli and solutes to tile drains via preferential and matrix flow. [Masters Thesis]. Oregon State University; 2002. Available from: http://hdl.handle.net/1957/29676

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