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Universidad de Cantabria
1.
Márquez López, Alicia.
Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.
Degree: 2016, Universidad de Cantabria
URL: http://hdl.handle.net/10902/8428
► RESUMEN: Caracterización molecular de aislamientos de Escherichia coli procedentes de distintas muestras clínicas para tratar de evaluar la relación entre la resistencia a quinolonas y…
(more)
▼ RESUMEN: Caracterización molecular de aislamientos de
Escherichia coli procedentes de distintas muestras clínicas para tratar de evaluar la relación entre la resistencia a quinolonas y la producción de hemolisinas. Encontramos diferencias en la presencia de los genes de la alfa-hemolisina (hlyA) y de la citolisina A (clyA), la actividad hemolítica, el perfil de sensibilidad a quinolonas y el grupo filogenético. hlyA se asoció con la actividad hemolítica a las 18-20 h de incubación, la sensibilidad a ácido nalidíxico y ciprofloxacino, y el grupo filogenético B2. clyA se relacionó con una actividad hemolítica parcial transcurridas 24 h tras una previa incubación, la resistencia a ácido nalidíxico y ciprofloxacino, y se distribuyó ampliamente dentro de todos los grupos filogenéticos, aunque en menor proporción en el grupo B2. Adicionalmente, evaluamos el papel patogénico de la citolisina A en dos modelos animales, y los resultados obtenidos ponen en duda su posible papel aislado como factor de virulencia en E.
coli.
Advisors/Committee Members: Martínez Martínez, Luis (advisor), Universidad de Cantabria (other).
Subjects/Keywords: Escherichia coli
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APA (6th Edition):
Márquez López, A. (2016). Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. (Doctoral Dissertation). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/8428
Chicago Manual of Style (16th Edition):
Márquez López, Alicia. “Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.” 2016. Doctoral Dissertation, Universidad de Cantabria. Accessed February 26, 2021.
http://hdl.handle.net/10902/8428.
MLA Handbook (7th Edition):
Márquez López, Alicia. “Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli.” 2016. Web. 26 Feb 2021.
Vancouver:
Márquez López A. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. [Internet] [Doctoral dissertation]. Universidad de Cantabria; 2016. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10902/8428.
Council of Science Editors:
Márquez López A. Resistencia a quinolonas y producción de hemolisinas en aislamientos clínicos de E. coli. [Doctoral Dissertation]. Universidad de Cantabria; 2016. Available from: http://hdl.handle.net/10902/8428

California State University – East Bay
2.
Herbold, Nicole M.
Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.
Degree: MSin Biological Sciences, 2014, California State University – East Bay
URL: http://hdl.handle.net/10211.3/130388
► There is a continuous need for improved accuracy and rapidity for detection methods applicable to foodborne pathogens; especially non-O157 Escherichia coli. Many E. coli, such…
(more)
▼ There is a continuous need for improved accuracy and rapidity for
detection methods applicable to foodborne pathogens; especially non-O157
Escherichia coli. Many E.
coli, such as E.
coli O157, produce Shiga toxin (STEC)
and are associated with several human foodborne illnesses that cause severe
diarrheal diseases, such as hemorrhagic colitis (HC) and hemolytic-uremic
syndrome (HUS). Methods commonly used to detect foodborne pathogens
employed by various food safety agencies include sample enrichment,
biochemical assays, real-time PCR (q-PCR), Pulse-field gel electrophoresis
(PFGE), plate analyses, and agglutination testing. One of the objectives of
this thesis research is to determine the feasibility and reliability for use of using
Immunomagnetic Separation (IMS) System, Fourier Transform Infrared (FT-IR)
Spectroscopy, and/or repetitive-PCR (rep-PCR) using the DiversiLab??? system
as effective and efficient methods for identification of pathogenic strains of E.
coli.
This thesis research tests some of the same pathogenic E.
coli strains across all
of the aforementioned methods, and each of these methods has shown to be
highly specific and accurate in detecting the various pathogenic E.
coli used.
Advisors/Committee Members: Lauzon, Dr. Carol R. (advisor), Uhde_Stone, Dr. Claudia (committee member).
Subjects/Keywords: Escherichia coli
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APA ·
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APA (6th Edition):
Herbold, N. M. (2014). Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. (Masters Thesis). California State University – East Bay. Retrieved from http://hdl.handle.net/10211.3/130388
Chicago Manual of Style (16th Edition):
Herbold, Nicole M. “Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.” 2014. Masters Thesis, California State University – East Bay. Accessed February 26, 2021.
http://hdl.handle.net/10211.3/130388.
MLA Handbook (7th Edition):
Herbold, Nicole M. “Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli.” 2014. Web. 26 Feb 2021.
Vancouver:
Herbold NM. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. [Internet] [Masters thesis]. California State University – East Bay; 2014. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10211.3/130388.
Council of Science Editors:
Herbold NM. Methods for Rapidly and Accurately Detecting Pathogenic Escherichia coli. [Masters Thesis]. California State University – East Bay; 2014. Available from: http://hdl.handle.net/10211.3/130388

Rutgers University
3.
Jang, Hye In, 1985-.
Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.
Degree: PhD, Food Science, 2017, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/
► Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts…
(more)
▼ Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts for pathogens. In order to enhance safety of fresh produce, it is important to understand the interactions between human enteric pathogens and plants. However, little information is available about the behavior of human enteric pathogens on plants, such as mechanisms of survival and persistence. In this study, we investigated survival and persistence of Escherichia coli O157:H7 and O104:H4 strains on Arabidopsis thaliana and romaine lettuce, as well as production of capsular polysaccharide (CPS) and induction of plant defense response. Colonization study with E. coli O157:H7 86-24 wild-type strain and its isogenic mutants of surface polysaccharides showed that colanic acid-deficient and lipopolysaccharide (LPS)-deficient mutants significantly less survived on Arabidopsis plant and lettuce on day 1 and 5 post-inoculation, compared to the wild-type. The two mutants of colanic acid and LPS induced 2-fold greater PR1 gene expression and produced significantly lower amount of CPS compared to wild-type strain (P < 0.05). The results may suggest that structures of colanic acid and LPS of E. coli O157:H7 influence the plant defense response, thereby resulting in different survival and colonization on plants. To investigate fitness of an emerging Shiga toxin-producing E. coli (STEC), colonization of E. coli O104:H4 strains on plants were compared with of that of E. coli O157:H7 strains. Results showed that E. coli O104:H4 strains (RG1, C3493, and LpfA) significantly survived better than E. coli O157:H7 strains (7386 and sakai) on Arabidopsis plant and lettuce at day 5, with greater production of CPS and lower expression of PR1 gene (P < 0.05). These results indicate that different level of plant defense response and CPS production may have an impact on survival or fitness of E. coli O104:H4 and O157:H7 on plants. In order to develop control strategies in crop cultivation environments, it is essential to learn about the behavior of human enteric pathogens on plants, particularly factors influencing the ability of pathogen to overcome plant host immunity. The present study provides better understanding of roles of plant defense response and surface polysaccharides on the molecular interactions between human pathogens and plants. Interestingly, the similar trend of bacterial survival/persistence between Arabidopsis (model plant) and lettuce (plant crop) may suggest a potential use of Arabidopsis as an appropriate model plant for studying the mechanisms of plant responses to human enteric pathogens on leafy vegetables. This study also provides an insight into potential roles of CPS in the survival of human enteric pathogens.
Advisors/Committee Members: Matthews, Karl R (chair), School of Graduate Studies.
Subjects/Keywords: Escherichia coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Jang, Hye In, 1. (2017). Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55512/
Chicago Manual of Style (16th Edition):
Jang, Hye In, 1985-. “Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.” 2017. Doctoral Dissertation, Rutgers University. Accessed February 26, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/55512/.
MLA Handbook (7th Edition):
Jang, Hye In, 1985-. “Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants.” 2017. Web. 26 Feb 2021.
Vancouver:
Jang, Hye In 1. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2021 Feb 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/.
Council of Science Editors:
Jang, Hye In 1. Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55512/
4.
Renata Katsuko Takayama Kobayashi.
Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.
Degree: 2006, Universidade Estadual de Londrina
URL: http://189.90.64.145/document/?code=vtls000112627
► Escherichia coli is an important agent of extra-intestinal infections both in humans and animals alike. In poultry, it is responsible for significant economic loss, being…
(more)
▼ Escherichia coli is an important agent of extra-intestinal infections both in humans and animals alike. In poultry, it is responsible for significant economic loss, being known as the pathogenic avian E. coli (APEC); and it causes colibacillosis, which starts as an infection of the respiratory tract evolving to airsacculitis, pericarditis, perihepatitis and septicemia. Several virulence genes are involved, among which tsh. The tsh gene codifies the autotransporter protein temperature sensitive hemagglutinin (Tsh), which has hemagglutinating and proteolytic activities. Tsh is synthesized as 140 kDa precursor protein, whose processing results in 106 kDa passenger domain (Tshs) and 33 kDa beta-domain (Tshb). In our study, we observed that these proteins presence is dependent on temperature and on the medium utilized for bacterial growth. We also verified that both the recombinant Tsh (140 kDa) and the pellets from wild sample APEC 13, which contain Tshb (33 kDa), agglutinated chicken erythrocytes. Both the recombinant Tsh (140kDa) and the supernatants from APEC 13, which contain Tshs (106 kDa), caused proteolysis of mucin. Chicken anti-Tsh serum inhibited the hemagglutinating activity of strains APEC 13 and recombinant E. coli BL21/pET101-tsh, and it also inhibited the mucinolytic activity of Tsh protein. The proof of Tsh protein mucinolytic activity was achieved by means of SDS-PAGE in gels copolymerized with mucins from different sources.
Escherichia coli é um agente importante de infecções extraintestinais em humanos e em animais. Em aves, é responsável por grandes perdas econômicas, sendo conhecida como E. coli patogênica para aves (APEC), e causa a colibacilose, a qual inicia como infecção do trato respiratório e evolui para aerosaculite, pericardite, perihepatite e septicemia. Vários genes de virulência estão envolvidos, sendo tsh um deles. O gene tsh codifica a proteína autotransportada hemaglutinina temperatura sensível (Tsh), que tem atividade hemaglutinante e proteolítica. Tsh é sintetizada como uma proteína precursora de 140 kDa, que é clivada, em um domínio passageiro de 106 kDa (Tshs) e um beta-domínio de 33 kDa (Tshb). Em nosso trabalho observamos que a presença destas proteínas é dependente da temperatura e do meio de cultura utilizado para o crescimento bacteriano. Observamos ainda que, tanto a Tsh recombinante (140 kDa) quanto o sedimento da amostra selvagem APEC 13, que contém Tshb (33 kDa), aglutinaram eritrócitos de galinha. Tanto a Tsh recombinante (140kDa) quanto o sobrenadante da APEC 13, que contém Tshs (106 kDa), causam proteólise da mucina. O anti-Tsh do soro de galinha inibiu a hemaglutinação da APEC 13 e da recombinante E. coli BL21/pET101-tsh, e inibiu também a atividade mucinolítica da proteína Tsh. A comprovação da atividade mucinolítica da proteína Tsh, foi possível através de SDS-PAGE em géis copolimerizados com mucinas de diferentes origens.
Advisors/Committee Members: Luiz Carlos Jabur Gaziri ., Ermerson José Venancio, Halha Ostrensky Saridakis, Ionice Felipe, Marilda Carlos Vidotto.
Subjects/Keywords: Escherichia coli; Escherichia coli; Microbiologia; Microbiology
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MLA ·
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Export
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APA (6th Edition):
Kobayashi, R. K. T. (2006). Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. (Thesis). Universidade Estadual de Londrina. Retrieved from http://189.90.64.145/document/?code=vtls000112627
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kobayashi, Renata Katsuko Takayama. “Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.” 2006. Thesis, Universidade Estadual de Londrina. Accessed February 26, 2021.
http://189.90.64.145/document/?code=vtls000112627.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kobayashi, Renata Katsuko Takayama. “Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli.” 2006. Web. 26 Feb 2021.
Vancouver:
Kobayashi RKT. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. [Internet] [Thesis]. Universidade Estadual de Londrina; 2006. [cited 2021 Feb 26].
Available from: http://189.90.64.145/document/?code=vtls000112627.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kobayashi RKT. Análise da expressão e caracterização funcional da hemaglutinina temperatura sensível de Escherichia coli. [Thesis]. Universidade Estadual de Londrina; 2006. Available from: http://189.90.64.145/document/?code=vtls000112627
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – La Cross
5.
Rentschler, Anne.
In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.
Degree: 2010, University of Wisconsin – La Cross
URL: http://digital.library.wisc.edu/1793/49107
► Uropathogenic Escherichia coli (UPEC) cause more than 90% of all human urinary tract infections (UTIs). Type 1 pili on the surface of UPEC allow the…
(more)
▼ Uropathogenic
Escherichia coli (UPEC) cause more than 90% of all human urinary tract infections (UTIs). Type 1 pili on the surface of UPEC allow the cells to adhere to and invade bladder epithelial cells. The fimB and fimE genes encode site-specific recombinases that position an invertible element containing the promoter for the gene of the main structural subunit of the type 1 pili. To sustain a UTI, UPEC must be able to respond to changes in environmental pH and osmolality. The EnvZ/OmpR two-component regulatory system is involved in osmoregulation in E.
coli. To ascertain if OmpR directly bound to fimB or fimE gene promoters, DNase I footprinting was performed using OmpR or phosphorylated OmpR (OmpR-P) mixed with the fimB and fimE promoter regions of UPEC NU149. Both OmpR-P and OmpR bound weakly to one fimB promoter. However, neither OmpR nor OmpR-P bound to the fimE promoter region. Because there was weak binding to one fimB promoter, strain NU149 was grown in different pH and osmolality environments, total RNAs were extracted from each population, and converted to cDNAs. Real-time reverse transcriptase PCR showed no differences in ompR transcription among the different growth conditions. Conversely, Western blots showed a significant increase in OmpR protein when the UPEC were grown in a combined acidic pH/high osmolality environment versus a neutral pH/high osmolality environment. Together these results suggest that OmpR may be post-transcriptionally regulated in UPEC grown in an acidic pH/high osmolality environment which may affect fimB regulation in UPEC.
Advisors/Committee Members: Stemper, Mary, Jobe, Dean, Rott, Marc, Schwan, William.
Subjects/Keywords: Escherichia coli infections
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rentschler, A. (2010). In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. (Thesis). University of Wisconsin – La Cross. Retrieved from http://digital.library.wisc.edu/1793/49107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rentschler, Anne. “In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.” 2010. Thesis, University of Wisconsin – La Cross. Accessed February 26, 2021.
http://digital.library.wisc.edu/1793/49107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rentschler, Anne. “In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli.” 2010. Web. 26 Feb 2021.
Vancouver:
Rentschler A. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. [Internet] [Thesis]. University of Wisconsin – La Cross; 2010. [cited 2021 Feb 26].
Available from: http://digital.library.wisc.edu/1793/49107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rentschler A. In vitro analysis of OmpR regulation of the fimB and fimE genes of Uropathogenic Escherichia coli. [Thesis]. University of Wisconsin – La Cross; 2010. Available from: http://digital.library.wisc.edu/1793/49107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

California State University – East Bay
6.
Greer, Jameelah.
Construction and Purification of N-terminal His6 YqgF Clone.
Degree: MSin Chemistry, 2015, California State University – East Bay
URL: http://hdl.handle.net/10211.3/137874
► The main goal of the study is to produce high levels of the YqgF protein for use in protein-protein interaction studies with YqgE. The specific…
(more)
▼ The main goal of the study is to produce high levels of the YqgF protein for use in
protein-protein interaction studies with YqgE. The specific experimental goals are:
1. To use the Polymerase Chain Reaction (PCR) technique to isolate and amplify the
full DNA sequence of yqgF.
2. To clone yqgF into a plasmid that includes a 6X His tag that will attach to the N-terminus
of the corresponding YqgF protein. Clones will be tested for the correct
orientation and size using Colony PCR and DNA sequencing.
3. To induce high level protein expression of the cloned yqgF genes in E.
coli cells.
Western Blotting will be used to detect the expressed protein in a crude extract.
4. To purify the YqgF fusion protein.
Advisors/Committee Members: McPartland, Dr. Ann (advisor), Sommerhalter, Dr. Monika (committee member), Student (sonomaclassification).
Subjects/Keywords: Escherichia coli – Genetics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Greer, J. (2015). Construction and Purification of N-terminal His6 YqgF Clone. (Masters Thesis). California State University – East Bay. Retrieved from http://hdl.handle.net/10211.3/137874
Chicago Manual of Style (16th Edition):
Greer, Jameelah. “Construction and Purification of N-terminal His6 YqgF Clone.” 2015. Masters Thesis, California State University – East Bay. Accessed February 26, 2021.
http://hdl.handle.net/10211.3/137874.
MLA Handbook (7th Edition):
Greer, Jameelah. “Construction and Purification of N-terminal His6 YqgF Clone.” 2015. Web. 26 Feb 2021.
Vancouver:
Greer J. Construction and Purification of N-terminal His6 YqgF Clone. [Internet] [Masters thesis]. California State University – East Bay; 2015. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10211.3/137874.
Council of Science Editors:
Greer J. Construction and Purification of N-terminal His6 YqgF Clone. [Masters Thesis]. California State University – East Bay; 2015. Available from: http://hdl.handle.net/10211.3/137874

University of Illinois – Urbana-Champaign
7.
Li, Jinbei Walden.
Development of in vivo ppGpp reporters in escherichia coli.
Degree: MS, Bioengineering, 2018, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/102515
► ppGpp is a small molecule that works as a global master regulator of the E. coli physiology, most notably during the stringent response. Systems biology…
(more)
▼ ppGpp is a small molecule that works as a global master regulator of the E.
coli physiology, most notably during the stringent response. Systems biology studies have emphasized its role in understanding the host response to the burden of introduced synthetic genetic circuits. Traditionally, researchers rely on in vitro methods to measure the intracellular ppGpp levels. In vivo ppGpp reporters, however, would allow the possibility of closely monitoring the ppGpp levels during different processes, especially the host response given the functioning of synthetic circuits with relatively fast dynamics. In this study, a series of in vivo ppGpp reporters were developed in E.
coli by placing a fluorescent protein under the control of promoters from genes known to be controlled by ppGpp levels. Reporter performance was tested by monitoring the fluorescence level with microscope during different stages of growth, induced stringent response, and different growth conditions. The four positive ppGpp reporters showed strong positive correlation with ppGpp levels, including two with particularly high sensitivity and signal intensity. The testing process also shed new light on the dynamics of ppGpp production during batch culture growth and under nutrient limitation. With further development, these in vivo ppGpp reporters promise to be very useful when studying the dynamics of host resource partitioning.
Advisors/Committee Members: Lu, Ting (advisor).
Subjects/Keywords: ESCHERICHIA COLI; ppGpp
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, J. W. (2018). Development of in vivo ppGpp reporters in escherichia coli. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/102515
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Jinbei Walden. “Development of in vivo ppGpp reporters in escherichia coli.” 2018. Thesis, University of Illinois – Urbana-Champaign. Accessed February 26, 2021.
http://hdl.handle.net/2142/102515.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Jinbei Walden. “Development of in vivo ppGpp reporters in escherichia coli.” 2018. Web. 26 Feb 2021.
Vancouver:
Li JW. Development of in vivo ppGpp reporters in escherichia coli. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/2142/102515.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li JW. Development of in vivo ppGpp reporters in escherichia coli. [Thesis]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/102515
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne
8.
Ingle, Danielle Joy.
Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.
Degree: 2016, University of Melbourne
URL: http://hdl.handle.net/11343/122899
► Atypical enteropathogenic Escherichia coli (aEPEC) is an important emerging pathogen that is causally associated with diarrhoea in children in both industrialised and developing nations. The…
(more)
▼ Atypical enteropathogenic Escherichia coli (aEPEC) is an important emerging pathogen that is causally associated with diarrhoea in children in both industrialised and developing nations. The current definition for aEPEC is the presence of the locus of enterocyte effacement (LEE) pathogenicity island and the absence of genes for bundle forming pili (bfpA) and Shiga toxin (stx). Classical approaches to categorise clinical isolates of aEPEC have shown that they are heterogeneous for sequence type, lipopolysaccharide (O-) and flagella (H-) surface antigens, antimicrobial resistance profiles and virulence genes.
Associations of aEPEC with diarrhoea are inconsistent, suggesting that the current definition of aEPEC lacks the ability to distinguish between virulent and less virulent isolates.
For this thesis, I used comparative genomic analysis to explore the evolution and population structure of aEPEC. A total of 196 putative aEPEC isolates were collected during the Global Enteric Multicenter Study (GEMS) from seven countries in South Asia and Sub-Saharan Africa. These isolates were subjected to whole genome sequencing and 185 were con rmed in silico as aEPEC. The core aEPEC genomes were rst compared with publicly available genomes with phylogenomic analysis revealing ten globally distributed clonal lineages of aEPEC.
Investigation into the evolution of the LEE showed that aEPEC clones were associated with different LEE subtypes. Analysis of genetic variation within the LEE revealed three major lineages, comprised of a total of 30 different subtypes. The three LEE lineages were shown to have different preferred tRNA insertion sites on the chromosome and to be associated with different complements of known non-LEE-encoded effector genes. The genes encoding the type III secretion system (T3SS) were found to limited in their evolution whilst genes encoding immunogenic proteins have accumulated extensive genetic diversity suggesting that they are subject to diversifying selection. This genetic variation was utilised in the development of a LEE typing scheme, the utility of which was demonstrated using isolates from public health investigations.
The diversity of O- and H-antigens was explored in the aEPEC collection, and a method as developed to facilitate in silico serotyping from short read data. This approach was validated by serological phenotyping of 197 EPEC isolates, before being used to characterise other clinically relevant isolates.
Antimicrobial resistance of GEMS aEPEC isolates was characterised phenotypically and genetically, revealing high levels of resistance across geographical sites and clonal lineages. Regional differences between the GEMS sites, that may be due to differences in local drug use, was determined to be a key driver for the acquisition and retention of gene content conferring antimicrobial resistance. Antimicrobial resistance could be reliably predicted from genome data for the majority of drug classes.
In summary, this study examined the evolution of the emerging pathogen, aEPEC…
Subjects/Keywords: Escherichia coli; genomics
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APA (6th Edition):
Ingle, D. J. (2016). Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/122899
Chicago Manual of Style (16th Edition):
Ingle, Danielle Joy. “Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.” 2016. Doctoral Dissertation, University of Melbourne. Accessed February 26, 2021.
http://hdl.handle.net/11343/122899.
MLA Handbook (7th Edition):
Ingle, Danielle Joy. “Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli.” 2016. Web. 26 Feb 2021.
Vancouver:
Ingle DJ. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. [Internet] [Doctoral dissertation]. University of Melbourne; 2016. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/11343/122899.
Council of Science Editors:
Ingle DJ. Evolution of an emerging pathogen: atypical enteropathogenic Escherichia coli. [Doctoral Dissertation]. University of Melbourne; 2016. Available from: http://hdl.handle.net/11343/122899

Universidad Andrés Bello
9.
García Mena, Nicole.
Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido
.
Degree: 2017, Universidad Andrés Bello
URL: http://repositorio.unab.cl/xmlui/handle/ria/6855
► El objetivo del proyecto es mejorar la eficiencia catalítica que posee la enzima Pglucuronidasa de Escherichia coli por codefna-6-glucurónido, a fin de reducir el tiempo…
(more)
▼ El objetivo del proyecto es mejorar la eficiencia catalítica que posee la enzima Pglucuronidasa
de
Escherichia coli por codefna-6-glucurónido, a fin de reducir el tiempo de
hidrólisis de éste metabolito en test de doping (actualmente se logra niveles de hidrólisis deseable
en 12 horas). Asumiendo que la eficiencia catalítica de la enzima se correlaciona con la energía
de unión del ligando en una confonnación catalftica, lo que se busca en esta tesis es generar
mutantes que mejoren la afinidad por codeína-6-glucurónido en una confonnación catalltica
similar al primer estado de transición del mecanismo catalftico. A modo comparativo, se partió el
análisis utilizando también morfina-3-glucurónido, para la cual se conoce que la enzima tiene una
eficiencia cataHtica mayor que por codefna-6-glucurónido.
En el Protein Data Bank (PDB) se encuentran 6 cristales de la enzima con diversos rangos
de resolución y largos de secuencia. Para identificar el confónnero que mejor sirva de templado
para nuestros estudios de unión de ligandos en confonnaciones catalíticas, se tomó cada una de
las cadenas presentes en los 6 cristales del PDB y se realizó ensayos de acoplamiento molecular
( docking) de cada uno de las cadenas con ligandos conocidos ( codefna-6-glucurónido y morfina-
3-glucurónido) usando el programa Autodock Vina. El procedimiento anterior arrojó la cadena A
de la estructura 3LPF como el mejor confónnero a partir del cual se llevó a cabo un proceso in
silico de generación de mutaciones simples y múltiples usando Modeller de fonna automatizada.
Para seleccionar los residuos del sitio activo que serían mutados se utilizó el mejor modelo de
docking de 3LPF cadena A y morfina-3-glucurónido o codefna-6-glucurónido, y se identificó los
residuos aminoacfdicos que estaban en proximidad de los ligandos usando VMD. Con la
selección de las mejores mutantes simples, se generaron luego mutantes dobles y triples con
criterios específicos de selección.
Finalmente, evaluamos si las posiciones mutadas que resultan en mejoras en la unión de
codefna-6-glucurónido en confonnaciones catalfticas ocurren en sitios con adaptación positiva
(aquellos donde las mutaciones no sinónimas priman por sobre las mutaciones sinónimas) o sitios
con adaptación negativa (mutaciones sinónimas predominan por sobre las no sinónimas). Existe
evidencia en la literatura para otras familias de enzimas que los sitios con adaptación positiva
están involucrados en cambios de especificidad de sustrato, por tanto, mutaciones racionales en sitios con adaptación positiva reflejarían de mejor manera la evolución natural que seguiría esta
enzima en un organismo confrontado en la naturaleza con el sustrato de nuestro interés. Para
cumplir este objetivo, se identificaron miembros caracterizados de la familia Glicosil Hidrolasa 2
a la que pertenece la P-glucuronidasa de
Escherichia coli. A partir de estos se generó una red de
similitud de secuencias con el fin de identificar otros miembros de la familia a partir de bases de
datos públicas de…
Advisors/Committee Members: Almonacid, Daniel (advisor).
Subjects/Keywords: Escherichia Coli;
Enzimas
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
García Mena, N. (2017). Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido
. (Thesis). Universidad Andrés Bello. Retrieved from http://repositorio.unab.cl/xmlui/handle/ria/6855
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
García Mena, Nicole. “Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido
.” 2017. Thesis, Universidad Andrés Bello. Accessed February 26, 2021.
http://repositorio.unab.cl/xmlui/handle/ria/6855.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
García Mena, Nicole. “Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido
.” 2017. Web. 26 Feb 2021.
Vancouver:
García Mena N. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido
. [Internet] [Thesis]. Universidad Andrés Bello; 2017. [cited 2021 Feb 26].
Available from: http://repositorio.unab.cl/xmlui/handle/ria/6855.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
García Mena N. Re-ingeniería de la B-glucuronidasa de E. coli para aumentar su eficiencia catalitica por codeína-6-glucurónido
. [Thesis]. Universidad Andrés Bello; 2017. Available from: http://repositorio.unab.cl/xmlui/handle/ria/6855
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University
10.
Basu, Debaleena.
The role of the A1 subunit in the activity of shiga toxins.
Degree: PhD, Plant Biology, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/
► Shiga toxin (Stx) producing E.coli infections can lead to life-threatening complications, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Stx1 and Stx2 are AB5 toxins…
(more)
▼ Shiga toxin (Stx) producing E.coli infections can lead to life-threatening complications, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Stx1 and Stx2 are AB5 toxins consisting of an enzymatically active A subunit and a pentamer of receptor binding B subunits. Stx2-producing E.coli strains are more frequently associated with HUS than Stx1-producing strains. The role of the A subunits in the increased potency of Stx2 has not been fully investigated. This study using purified A1 subunits, provide the first direct evidence that the higher affinity for ribosomes in combination with higher catalytic activity towards the SRL allows Stx2A1 to depurinate ribosomes, inhibit translation and exhibit cytotoxicity at a significantly higher level than Stx1A1 in yeast and human cells. To determine if conserved arginines at the distal face of the active site contribute to the higher affinity of Stx2A1 for the ribosome, Arg172, Arg176 and Arg179 in Stx1A1 and Stx2A1 are mutated. It is seen that Arg172 and Arg176 are more important than Arg179 for depurination activity and toxicity of Stx1A1 and Stx2A1. Mutation of at least two of the three arginines is required to significantly reduce depurination by Stx2A1 in vitro and in cells in yeast and mammalian cells. Conserved arginines at the distal face of the active site are critical for interactions of Stx1A1 and Stx2A1 with the stalk, while a conserved arginine at the active site is critical for non-stalk specific interactions with the ribosome. Mutations at conserved arginines at either site reduced ribosome interactions of Stx1A1 and Stx2A1 similarly, indicating that they do not contribute to the higher affinity of Stx2A1 for the ribosome. Interchanging residues E60 and 61 in Stx1A1 and Y60 and Q61 in Stx2A1 that are located away from the active site and ribosome stalk binding site, resulted in small but significant increase in depurination level of E60Y/E61Q for Stx1A1 and a decrease in depurination level of Y60E/Q61E of Stx2A1 in vivo in yeast. A larger difference may be observed if more than two residues are simultaneously altered to change the electrostatic charge distribution of Stx1A1 and Stx2A1 sufficiently.
Advisors/Committee Members: Tumer, Nilgun (chair), White, James (internal member), Belanger, Faith (internal member), Woychik, Nancy (outside member).
Subjects/Keywords: Escherichia coli; Toxins
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Basu, D. (2016). The role of the A1 subunit in the activity of shiga toxins. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51187/
Chicago Manual of Style (16th Edition):
Basu, Debaleena. “The role of the A1 subunit in the activity of shiga toxins.” 2016. Doctoral Dissertation, Rutgers University. Accessed February 26, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/51187/.
MLA Handbook (7th Edition):
Basu, Debaleena. “The role of the A1 subunit in the activity of shiga toxins.” 2016. Web. 26 Feb 2021.
Vancouver:
Basu D. The role of the A1 subunit in the activity of shiga toxins. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Feb 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/.
Council of Science Editors:
Basu D. The role of the A1 subunit in the activity of shiga toxins. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51187/

University of Aberdeen
11.
Galbiati Belmonte, Heloisa Filus.
Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.
Degree: PhD, 2016, University of Aberdeen
URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591
► Escherichia coli (E. coli) frequently experiences changes in environmental osmolarity thus requiring homeostatic adjustments for proper cell function. In a hypoosmotic shock the central players…
(more)
▼ Escherichia coli (E. coli) frequently experiences changes in environmental osmolarity thus requiring homeostatic adjustments for proper cell function. In a hypoosmotic shock the central players are the mechanosensitive channels (MS channels), considered the major routes for the release of cytoplasmic solutes to achieve rapid reduction of turgor pressure. In the present study a combination of in vivo and in vitro techniques were used to investigate three of the seven MS channels present in the genome of E. coli, these channels being MscL, MscS and MscK. The first chapter focused on three residues (R46, R59 and K60) in the transmembrane helices TM1 and TM2 of MscS. It has been hypothesized that these residues act as part of the tension sensor mechanism, interacting either with membrane phospholipids or ions within the pore of the channel. In addition to MscS, the second chapter included MscL and MscK and analyzed the precise localization and diffusion rate of these proteins in the cytoplasmic membrane by the use of a super resolution fluorescence microscopy technique. The same technique was used in the third chapter to quantify the two main MS channels, MscL and MscS. As well as providing a single cell census these analyses provided a correlation between abundance of channels and cells survival during a downshock, the situation in which these channels gate and protect cells. Quantification revealed a high abundance of these proteins, thus MS channels were also investigated for an alternative role, namely the release of solutes during hyperosmotic shocks. This last chapter focused specifically on glutamate excretion, which is part of the hyperosmotic response. In summary, the results presented in this thesis substantially increased our knowledge of MS channels, both from a functional and a quantitative perspective.
Subjects/Keywords: 579.3; Escherichia coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Galbiati Belmonte, H. F. (2016). Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591
Chicago Manual of Style (16th Edition):
Galbiati Belmonte, Heloisa Filus. “Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.” 2016. Doctoral Dissertation, University of Aberdeen. Accessed February 26, 2021.
https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591.
MLA Handbook (7th Edition):
Galbiati Belmonte, Heloisa Filus. “Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis.” 2016. Web. 26 Feb 2021.
Vancouver:
Galbiati Belmonte HF. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. [Internet] [Doctoral dissertation]. University of Aberdeen; 2016. [cited 2021 Feb 26].
Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591.
Council of Science Editors:
Galbiati Belmonte HF. Mechanosensitive channels in Escherichia coli : a functional and quantitative analysis. [Doctoral Dissertation]. University of Aberdeen; 2016. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152504840005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.690591

Rutgers University
12.
Keklak, Julia T., 1991-.
The effect of substrate stiffness on uropathogenic E. coli infection.
Degree: MS, Biology, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/
► Both mammalian and bacterial cells respond to environmental cues and then elicit various physiological responses. Mechanical cues, such as surface attachment, have been noted as…
(more)
▼ Both mammalian and bacterial cells respond to environmental cues and then elicit various physiological responses. Mechanical cues, such as surface attachment, have been noted as a critical part of bacterial infection through interaction with mechanosensors and the actin cytoskeleton. Urinary tract infections are a sufficient model for studying attachment due to the mechanism of bacterial infection. The cytoskeleton mediates various cell processes that are involved with infection, such as endocytosis and vesicle trafficking. The disruption of the host actin cytoskeleton leads to increased uropathogenic E.
coli infection (UPEC). Since substrate stiffness mediates changes in host cell physiology through cytoskeletal interaction, we explored the UPEC infection process on polyacrylamide gels of differing stiffnesses. We found that UPEC escapes endocytic vesicles and replicates within the cytoplasm on soft gels, yet are trapped within lysosomal-associated membrane protein (LAMP-1) vesicles on stiff surfaces. Cytoplasmic replication is consistent with previous in vivo studies. Increased infection was also found on soft gels when compared with stiff substrates. These findings may provide a more accurate cell culturing model for future UPEC infection studies.
Advisors/Committee Members: Klein, Eric A (chair), Yakoby, Nir (internal member), Nam, Jongmin (internal member).
Subjects/Keywords: Escherichia coli infections
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Keklak, Julia T., 1. (2016). The effect of substrate stiffness on uropathogenic E. coli infection. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49806/
Chicago Manual of Style (16th Edition):
Keklak, Julia T., 1991-. “The effect of substrate stiffness on uropathogenic E. coli infection.” 2016. Masters Thesis, Rutgers University. Accessed February 26, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/49806/.
MLA Handbook (7th Edition):
Keklak, Julia T., 1991-. “The effect of substrate stiffness on uropathogenic E. coli infection.” 2016. Web. 26 Feb 2021.
Vancouver:
Keklak, Julia T. 1. The effect of substrate stiffness on uropathogenic E. coli infection. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Feb 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/.
Council of Science Editors:
Keklak, Julia T. 1. The effect of substrate stiffness on uropathogenic E. coli infection. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49806/

Ryerson University
13.
Gadishaw-Lue, Crystal.
Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.
Degree: 2015, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347
► Enterohemorrhagic Escherichia coli (EHEC) causes severe food and water-borne illness associated with diarrhea, hemorrhagic colitis (HC), and hemolytic-uremic syndrome (HUS). Previously, we reported that treatment…
(more)
▼ Enterohemorrhagic Escherichia coli (EHEC) causes severe food and water-borne illness associated with diarrhea, hemorrhagic colitis (HC), and hemolytic-uremic syndrome (HUS). Previously, we reported that treatment of EHEC with a physiologically relevant bile salt mixture (BSM) upregulates genes encoding a two-component system (TCS) (basRS) and a lipid A modification pathway (arnBCADTEF). The current study examines the effect of BSM treatment on EHEC resistance to human cationic antimicrobials, human defensin, HD-5 and cathelicidin, LL-37. Results show a significant increase in resistance to HD-5 when EHEC are pre-treated with BSM as compared to untreated EHEC. The BS-induced resistance phenotype is lost in each of the arnT and basS mutants. Interestingly, BSM treatment does not affect resistance to LL-37. The results of this study provide evidence that BS serve as an environmental cue by triggering changes via a TCS that result in protective modifications of the bacterial outer membrane, thereby increasing resistance to HD-5.
Subjects/Keywords: Bile salts; Escherichia coli 0157:H7; Escherichia coli infections; Escherichia coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gadishaw-Lue, C. (2015). Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A4347
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gadishaw-Lue, Crystal. “Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.” 2015. Thesis, Ryerson University. Accessed February 26, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A4347.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gadishaw-Lue, Crystal. “Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides.” 2015. Web. 26 Feb 2021.
Vancouver:
Gadishaw-Lue C. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. [Internet] [Thesis]. Ryerson University; 2015. [cited 2021 Feb 26].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gadishaw-Lue C. Bile salts differentially enhance resistance of enterohemorrhagic escherichia coli 0157:H7 to human cationic antimicrobial peptides. [Thesis]. Ryerson University; 2015. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A4347
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
14.
Silva, Mídana Felismino da.
Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.
Degree: 2009, Universidade da Beira Interior
URL: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048
► O aumento inabalável da resistência da E. coli aos antibióticos e as diferenças geográficas na sua prevalência levam com que seja imprescindíveis os estudos locais…
(more)
▼ O aumento inabalável da resistência da E. coli aos antibióticos e as diferenças geográficas na sua prevalência levam com que seja imprescindíveis os estudos locais de prevalências e das tendências evolutivas das resistências. Objectivo: Analisar a prevalência e as tendências evolutivas da resistência da E. coli no distrito de Castelo Branco de Janeiro de 2006 a Dezembro de 2008 Método: Trata-se de um estudo descritivo com uma abordagem quantitativa. Foram analisadas todos os resultados dos testes de sensibilidade aos antibióticos efectuados nos laboratórios de microbiologia do Centro Hospitalar da Cova da Beira e do Hospital Amato Lusitano, obtidos mediante sistemas automatizados para identificação e antibiograma (VITEK®) durante aquele período. Os isolados foram incluídos com base no perfil de sensibilidade e no tempo de isolamento. Resultados: Foram estudados 1138 isolados no HAL e 1714 no CHCB. Observou-se uma elevada taxa de resistência da E. coli à penicilina (> 40%) em ambos os hospitais durante os três anos em causa. Entre os isolados no CHCB observou-se uma tendência decrescente nas taxas de resistências entre 2007 e 2008, à excepção da taxa de resistência à ciprofloxacina que tende a aumentar. Relativamente a resistência dos isolados no HAL, observou-se uma tendência à estabilizar porém, as taxas de resistência à ampicilina e à gentamicina tendem a aumentar. No CHCB observou-se também uma reemergência dos isolados da E. coli produtores de ESBL. No HAL, apesar de se verificar uma ligeira diminuição na sua taxa de prevalência, esta ainda permanece elevada.
Conclusão: O presente trabalho revelou as diferenças nas variações evolutivas das taxas de prevalência da resistência da E. coli entre duas regiões do distrito de Castelo Branco à semelhança das encontradas em outras investigações em diferentes regiões e países. Comprovando a necessidade de desenvolvimento de um compromisso local constante, no combate as resistências bem como na adaptação dos protocolos de tratamentos às realidades locais.
Subjects/Keywords: Escherichia coli - Resistência antibiótica; Escherichia coli - Prevalência; Escherichia coli - Tratamento
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Silva, M. F. d. (2009). Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. (Thesis). Universidade da Beira Interior. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Silva, Mídana Felismino da. “Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.” 2009. Thesis, Universidade da Beira Interior. Accessed February 26, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Silva, Mídana Felismino da. “Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008.” 2009. Web. 26 Feb 2021.
Vancouver:
Silva MFd. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. [Internet] [Thesis]. Universidade da Beira Interior; 2009. [cited 2021 Feb 26].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Silva MFd. Escherichia coli e a resistência antibiótica : uma análise do padrão de evolução da resistência da Escherichia coli aos antibióticos no distrito de Castelo Branco, de 2006 a 2008. [Thesis]. Universidade da Beira Interior; 2009. Available from: http://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/1048
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas
15.
Verma, Renu, 1987-.
Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade.
Degree: 2016, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685
► Abstract: Brazil is the third largest producer and first exporter of poultry meat worldwide. Avian Pathogenic Escherichia coli (APEC) is responsible for significant economic losses…
(more)
▼ Abstract: Brazil is the third largest producer and first exporter of poultry meat worldwide. Avian Pathogenic
Escherichia coli (APEC) is responsible for significant economic losses in multi-million dollar in the industry, by causing systemic or localized diseases collectively known as colibacillosis. Besides its importance, the virulence mechanisms of these pathotypes have not been fully elucidated yet. Therefore, in this work, our aim was to study genes, which could be potentially involved in the pathogenicity of an APEC strain isolated from a chicken presenting clinical signs of Swollen Head Syndrome (strain SCI-07; nontypeable O: H31). Two genes yadC and yicS, which were demonstrated under positive selection, were chosen to be deleted and their contribution to several phenotypes related to pathogenesis process were further evaluated. For this, the mutated and complemented strains were compared to the wild type strain in several tests, such as mortality, motility, survival in macrophage, adhesion and invasion assays. The mutant yadC showed a decreased adhesion capacity in human epithelial (HeLa cells) and reduced ability in invasion to epithelial cells (Hep-2 cells) as well as decreased capacity of survival in avian macrophages (HD11 cells). The mutant also reduced mortality levels, as well as a reduced ability in the systemic animal infection experiment. The mutant yicS showed a decreased invasion level in CEC-32 cells and Hep-2 cell, reduced motility and a lower capacity of biofilm formation. This mutant also presented a decrease in mortality in one-day old-chicks model and systemic infection experiments. Taken together, these results indicate that these genes contribute to the infectious process and are important for the pathogenicity of strain SCI-07
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Dias da Silveira, Wanderley, 1956- (advisor), Rojas, Thaís Cabrera Galvão, 1980- (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Sircili, Marcelo Palma (committee member), Ramos, Marcelo de Carvalho (committee member), Cipriano, Matheus Aparecido Pereira (committee member), Paiva, Jacqueline Boldrin de (committee member).
Subjects/Keywords: Escherichia coli patogenica aviaria; Colibacilose; Escherichia coli - Patogenicidade; Deleção de genes; Proteínas de Escherichia coli; Avian pathogenic Escherichia coli; Escherichia coli infections; Escherichia coli - Pathogenicity; Gene deletion; Escherichia coli proteins
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Verma, Renu, 1. (2016). Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Verma, Renu, 1987-. “Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade.” 2016. Thesis, Universidade Estadual de Campinas. Accessed February 26, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Verma, Renu, 1987-. “Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade.” 2016. Web. 26 Feb 2021.
Vancouver:
Verma, Renu 1. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Feb 26].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Verma, Renu 1. Deletion of genes yadC and yicS from avian pathogenic Escherichia col (APEC) strain SCI-07 and their role in pathogenicity = Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade: Deleção dos genes yadC e yicS de uma linhagem SCI-07 de Escherichia coli patogênica para aves (APEC) e suas contribuições para a patogenicidade. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322685
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ryerson University
16.
Tollman, Hannah.
Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.
Degree: 2013, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861
► Escherichia coli (EHEC) 0157:H7 is a pathogenic bacterial species that is most commonly linked to severe diarrhea, but is also the leading cause of the…
(more)
▼ Escherichia coli (EHEC) 0157:H7 is a pathogenic bacterial species that is most commonly linked to severe diarrhea, but is also the leading cause of the potentially fatal hemolytic-uremic syndrome (HUS). In order to establish infection in the colon, EHEC must endure different stresses encountered in the gastrointestinal (GI) tract, such as acid stress in the stomach, bile salt stress in the small intestine, and short-chain fatty acid (SCFA) stress in the colon. These bacteria are likely able to use GI stresses as indicators of their location, impacting gene expression of adhesion, motility, and virulence factors. The E.
coli Common Pilus (ECP) has been shown to be an important factor for EHEC adhesion to epithelial cells, which is increased after either acid or SCFA stress. It has also been demonstrated via microarray that genes of this operon are upregulated after acid stress. The aim of this study is to determine how expression of the main subunit of this structure, EcpA, is regulated upon exposure of EHEC 0157:H7 to acid or SCFA-stress. Both transcriptional and translational regulation are hypothesized to be involved. Isogenic mutants have been constructed that lacked key regulators suspected to be important for each system. Two approaches are used to determine if the predicted regulatory systems are playing a role in response to stress: observing EcpA protein expression analysis through Western blotting with anti-EcpA antibodies, and examining differences in ecp operon promoter activity in regulatory mutants. In this study Western blots reconfirmed H-NS does not modulate ecpA expression in direct response to acute acid stress. This suggests an alternate regulatory response in EHEC 0157:H7 to acute acid stress resulting in the upregulation of ecpA expression previously observed with microarray analysis.
Advisors/Committee Members: Foster, Debora (Thesis advisor), Ryerson University (Degree grantor).
Subjects/Keywords: Escherichia coli; Escherichia coli infections; Escherichia coli O157:H7; Bacteria – Adhesion; Cell adhesion
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tollman, H. (2013). Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A2861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tollman, Hannah. “Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.” 2013. Thesis, Ryerson University. Accessed February 26, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A2861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tollman, Hannah. “Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress.” 2013. Web. 26 Feb 2021.
Vancouver:
Tollman H. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. [Internet] [Thesis]. Ryerson University; 2013. [cited 2021 Feb 26].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tollman H. Investigation of the regulation of expression of E. Coli Common Pilus subunit, ECPA, of enterohaemorrhagic E. Coli 0157:H7 under acid stress. [Thesis]. Ryerson University; 2013. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A2861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
17.
Laine, Matti.
Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon.
Degree: 2019, Theseus
URL: http://www.theseus.fi/handle/10024/262113
► Polymeraasiketjureaktio (PCR) on paljon käytetty menetelmä, jolla on nykyään monia erilaisia käyttökohteita ja se on keskeinen menetelmä molekyylibiologian laboratoriossa. PCR-reaktiossa hyvinkin pienestä määrästä DNA:ta saadaan…
(more)
▼ Polymeraasiketjureaktio (PCR) on paljon käytetty menetelmä, jolla on nykyään monia erilaisia käyttökohteita ja se on keskeinen menetelmä molekyylibiologian laboratoriossa. PCR-reaktiossa hyvinkin pienestä määrästä DNA:ta saadaan monistettua moninkertainen määrä samaa DNA:ta, jolloin sitä voidaan käyttää esimerkiksi diagnostisiin tarkoituksiin. Esimerkiksi mikrobiologian laboratoriossa PCR-tekniikkaa käytetään jo hyvin paljon, ja tekniikan käyttö vain lisääntyy. PCR-tekniikkaa avuksi käyttäen voidaan tunnistaa bakteereja ja monistaa DNA-jaksoja.
Kvantitatiivisella polymeraasiketjureaktiolla (qPCR) pystytään mittaamaan monistettavan DNA:n määrää reaaliajassa merkkiaineiden avulla. Menetelmän etuina on sen nopeus eikä se tarvitse agaroosigeeliä tulosten tulkintaan. Esimerkiksi tiettyjen bakteereiden osoituksessa siitä on tullut jo rutiinimenetelmä.
Opinnäytetyön tavoitteena on tuottaa työohje E. colin määrityksestä kvantitatiivisella PCR-menetelmällä. Opinnäytetyön tarkoituksena on tehdä StepOnePlus™ Real-Time PCR-analysaattorilla määritys, ja tuottaa prosessista toimiva työohje. Työohje tulee Tampereen ammattikorkeakoulun molekyylibiologian laboratorioon opetuskäyttöön. Opinnäytetyö toteutetaan toiminnallisena opinnäytetyönä.
Menetelmä testattiin Tampereen ammattikorkeakoulun molekyylibiologian laboratoriossa, ja opinnäytetyö sisältää tarkempaa tietoa prosessin kulusta ja eri työvaiheista. Prosessi lähtee liikkeelle bakteerin genomin eristyksestä päättyen qPCR-määrityksen tuloksiin.
Kirjallinen osio antaa pohjatietoa PCR:n perusperiaatteista ja käyttökohteista. Materiaali on hyväksi tueksi työohjeen käyttäjälle myös laboratorioharjoituksiin. Kirjallinen osio avaa opiskelijalle testauksen eri vaiheita, joita suoritettiin työohjeen toimivuuden takaamiseksi.
Kehitysehdotuksena työohjetta tulisi testata vielä opiskelijaryhmällä, ja muokata sitä saadun palautteen mukaan. Menetelmää voisi saada kustannustehokkaammaksi optimoimalla reaktion alukkeiden ja SYBR® Greenin määriä.
Polymerase chain reaction (pcr) is widely used as a diagnostic tool in molecular biology laboratory. PCR is able to amplify very small amounts of specific DNA sequence multiple times. This amplified DNA sequence can be used to detect different kinds of bacterial and viral infections. Quantitative PCR (qPCR) can be used to detect this DNA amplification in real-time using different kind of specific fluorescent dyes or probes.
The aim of this study was to create an instruction booklet on the detection of E. coli using qPCR analysis. This was accomplished by doing detection using StepOnePlus™ Real-time analyser and creating an instruction booklet based on that detection.
This study was conducted as a practice-based study. Before creation of the instruction booklet, two laboratory tests where performed. In the first one, the qPCR reaction was tested and checked if primers or reaction conditions needed optimising. In the second test genomic DNA of E. coli was extracted and quantified using analysis. An instruction booklet was…
Subjects/Keywords: PCR; qPCR; Escherichia coli; E. coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Laine, M. (2019). Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon. (Thesis). Theseus. Retrieved from http://www.theseus.fi/handle/10024/262113
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Laine, Matti. “Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon.” 2019. Thesis, Theseus. Accessed February 26, 2021.
http://www.theseus.fi/handle/10024/262113.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Laine, Matti. “Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon.” 2019. Web. 26 Feb 2021.
Vancouver:
Laine M. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon. [Internet] [Thesis]. Theseus; 2019. [cited 2021 Feb 26].
Available from: http://www.theseus.fi/handle/10024/262113.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Laine M. Escherichia colin määritys kvantitatiivisella PCR:llä : työohje molekyylibiologian laboratorioon. [Thesis]. Theseus; 2019. Available from: http://www.theseus.fi/handle/10024/262113
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – Oshkosh
18.
Fossen, Jenna L.
A Genomic View of Escherichia Coli Diversity from Dairy Calves.
Degree: 2019, University of Wisconsin – Oshkosh
URL: http://digital.library.wisc.edu/1793/79925
► Escherichia coli is a model organism for the scientific community but there are still numerous gaps in our knowledge, including its diversity within the gastrointestinal…
(more)
▼ Escherichia coli is a model organism for the scientific community but there are
still numerous gaps in our knowledge, including its diversity within the gastrointestinal
tract of dairy calves and those that survive within the farm microbiome. From this
particular animal reservoir, E.
coli can be transmitted into the food supply chain leading
to human disease if pathogenic strains are ingested. The aim of this study was to
determine if pathogenic strains of E.
coli are common in calf fecal samples and if the
calf’s diet and age plays a role in type and numbers of strains that are carried. Sixty-six
isolates were recovered from five dairy calves ranging from 5 hours to 3 months old.
Isolates were confirmed to be E.
coli through protein fingerprinting profiles. The highquality bacterial genomes (n=38) were further examined for virulence factors and were
classified into serogroups. These isolates displayed an average of seven different
virulence factors per isolate genome. Select housekeeping genes were examined to
determine isolate relatedness and genetic diversity. Seven to nine main clusters of
serotypes displayed together repeatedly. The occurrence of different E.
coli populations
corresponded to the 12 serogroup types observed. Nine isolates from one pre-weaned calf
all serotyped as O145:H28, a putative human pathogen, and their genomic variation
illustrated that these are most likely clonal. The occurrence of a pathogenic serotype is
particularly significant, pointing to a potential super shedder that could contaminate an
entire herd. While a majority of the isolates are predicted to be non-pathogenic, this
highlights the diversity among E.
coli strains even when calves are reared in the same
environment.
Advisors/Committee Members: Mueller-Spitz, Sabrina R..
Subjects/Keywords: Escherichia coli; E. coli; Dairy Calves; Genes
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fossen, J. L. (2019). A Genomic View of Escherichia Coli Diversity from Dairy Calves. (Thesis). University of Wisconsin – Oshkosh. Retrieved from http://digital.library.wisc.edu/1793/79925
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fossen, Jenna L. “A Genomic View of Escherichia Coli Diversity from Dairy Calves.” 2019. Thesis, University of Wisconsin – Oshkosh. Accessed February 26, 2021.
http://digital.library.wisc.edu/1793/79925.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fossen, Jenna L. “A Genomic View of Escherichia Coli Diversity from Dairy Calves.” 2019. Web. 26 Feb 2021.
Vancouver:
Fossen JL. A Genomic View of Escherichia Coli Diversity from Dairy Calves. [Internet] [Thesis]. University of Wisconsin – Oshkosh; 2019. [cited 2021 Feb 26].
Available from: http://digital.library.wisc.edu/1793/79925.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fossen JL. A Genomic View of Escherichia Coli Diversity from Dairy Calves. [Thesis]. University of Wisconsin – Oshkosh; 2019. Available from: http://digital.library.wisc.edu/1793/79925
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo
19.
Sukhija, Karan.
Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.
Degree: 2011, University of Waterloo
URL: http://hdl.handle.net/10012/5982
► A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-flippase recognition targets (FLP-FRT) recombinations was proposed for genomic engineering of Escherichia coli. For demonstration…
(more)
▼ A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-flippase recognition targets (FLP-FRT) recombinations was proposed for genomic engineering of Escherichia coli. For demonstration purposes, DNA operons containing heterologous genes (i.e. pac encoding E. coli penicillin acylase and palB2 encoding Pseudozyma antarctica lipase B mutant) engineered with regulatory elements, such as strong/inducible promoters (i.e. Ptrc and ParaB), operators, and ribosomal binding sites, were integrated into the E. coli genome at designated locations (i.e. lacZYA, dbpA, and lacI-mhpR loci) either as a gene replacement or gene insertion using various antibiotic selection markers (i.e. kanamycin and chloramphenicol) under various genetic backgrounds (i.e. HB101 and DH5α). The expression of the inserted foreign genes was subject to regulation using appropriate inducers [Isopropyl β-D-1-thiogalactopyranoside (IPTG) and arabinose] at tuneable concentrations. The developed approach has paved an effective way to “tailor” plasmid-free E. coli strains with desired genotypes suitable for various biotechnological applications, such as biomanufacturing and metabolic engineering.
Subjects/Keywords: metabolic engineering; e. coli; escherichia coli; recombineering
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sukhija, K. (2011). Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/5982
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sukhija, Karan. “Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.” 2011. Thesis, University of Waterloo. Accessed February 26, 2021.
http://hdl.handle.net/10012/5982.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sukhija, Karan. “Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains.” 2011. Web. 26 Feb 2021.
Vancouver:
Sukhija K. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. [Internet] [Thesis]. University of Waterloo; 2011. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10012/5982.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sukhija K. Advanced Genomic Engineering Strategy based on Recombineering Protocols to “Tailor” Escherichia coli Strains. [Thesis]. University of Waterloo; 2011. Available from: http://hdl.handle.net/10012/5982
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University
20.
Narwankar, Revati.
Effect of point mutations on the toxicity of shiga toxin A1 subunit.
Degree: MS, Food Science, 2019, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/
► Shiga toxin (Stx)-producing Escherichia coli (STEC) infections can lead to life- threatening complications, including hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS), which is the most…
(more)
▼ Shiga toxin (Stx)-producing
Escherichia coli (STEC) infections can lead to life- threatening complications, including hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS), which is the most common cause of acute renal failure in children in the United States. Previous studies have shown that STEC strains producing Stx2 are more commonly associated with HUS than those producing Stx1. Our lab has shown that the A1 subunit of Stx2 (Stx2A1) is more active than the A1 subunit of Stx1 (Stx1A1). The purpose of my study is to understand the basis for the higher toxicity of Stx2A1 by interchanging its residues with Stx1A1. Initially, the activity of wild type Stx1A1 and Stx2A1 was compared in yeast in order to establish a base line to study the effect of new mutations. We used site directed mutagenesis to exchange residues that contribute to surface charge differences between Stx1A1 and Stx2A1. Point mutations were made by interchanging Stx2A1 residues with Stx1A1 residues. If these residues contributed to the higher toxicity of Stx2A1 its toxicity would be reduced and the toxicity of Stx1A1 would be increased. The plasmids containing the mutants were transformed into yeast. The transformants were then grown in dextrose in a double overnight culture and expression was induced in galactose. The depurination activity and cytotoxicity of the point mutants was examined at different time points post-induction. Only small differences were observed between the single point mutants and the wild type toxins in both depurination activity and cytotoxicity. In contrast, several multiple mutations increased the toxicity of Stx1A1. While double mutations did not reduce the toxicity of Stx2A1 possibly due to its higher toxicity, quadruple and quintuple mutations reduced the toxicity of Stx2.
Advisors/Committee Members: Tumer, Nilgun E (chair), Schaffner, Donald W (internal member), Matthews, Karl R (internal member), School of Graduate Studies.
Subjects/Keywords: E. coli; Escherichia coli – Toxicology – Genetic aspects
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Narwankar, R. (2019). Effect of point mutations on the toxicity of shiga toxin A1 subunit. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61827/
Chicago Manual of Style (16th Edition):
Narwankar, Revati. “Effect of point mutations on the toxicity of shiga toxin A1 subunit.” 2019. Masters Thesis, Rutgers University. Accessed February 26, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/61827/.
MLA Handbook (7th Edition):
Narwankar, Revati. “Effect of point mutations on the toxicity of shiga toxin A1 subunit.” 2019. Web. 26 Feb 2021.
Vancouver:
Narwankar R. Effect of point mutations on the toxicity of shiga toxin A1 subunit. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Feb 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/.
Council of Science Editors:
Narwankar R. Effect of point mutations on the toxicity of shiga toxin A1 subunit. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61827/
21.
Magalhães, Pedro José Santana Ribeiro.
Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli.
Degree: 2016, RCAAP
URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046
► Dissertação de Mestrado em Biotecnologia para as Ciências da Saúde
A resistência a antibióticos é um problema gobal na medicina humana e veterinária. Escherichia coli…
(more)
▼ Dissertação de Mestrado em Biotecnologia para as Ciências da Saúde
A resistência a antibióticos é um problema gobal na medicina humana e veterinária. Escherichia coli é um microrganismo comensal do trato gastrointestinal de animais e humanos. Esta pode ser utilizada em diversos estudos biológicos e genéticos sobre a resistência aos antibióticos, um problema emergente de saúde pública. A presença do plasmídeo pMdT1 é de grande relevância no estudo da resistência aos antibióticos, visto que contém um gene que codifica uma variante da proteína AAC(6’)-lb-cr e que esta, quando ativa, confere resistência à tobramicina e à canamicina, e diminui a susceptibilidade à ciprofloxacina e à norfloxacina.
Foram utilizadas duas amostras, Escherichia coli Electromax DH10B, recetora do processo de transformação e Escherichia coli TF-Se20 sendo esta transformada, pois contém o plasmídeo pMdT1 que expressa o gene da acetilase. Após a extração proteica, a separação e quantificação das proteínas foi realizada mediante a eletroforese monodimensional e posteriormente bidimensional, segundo os princípios de O’Farrel, contudo usando a tecnologia de IPG. As amostras foram sujeitas a uma focalização isoelétrica, seguida de SDS-PAGE. A identificação das proteínas foi realizada através da técnica MALDI-TOF/MS. Os picos de massa obtidos pelo motor de busca Mascot foram comparados com a base de dados Uniprot e NCBI. Para determinar a sequência da proteína de interesse, utilizou-se a técnica LC-MS/MS.
Da amostra Escherichia coli TF-Se20 foram excisados 260 spots. Destes, foram identificados 111, e foi possível identificar 76 proteínas distintas, 71 das quais com função conhecida. Foram excisados 225 spots da amostra de Escherichia coli Electromax DH10B, tendo sido identificados 119, dos quais foi possível identificar 72 proteínas diferentes (71 têm processo biológico). As proteínas identificadas participam em diversos processos biológicos como no processo da glicólise, no processo de oxidação-redução e biossíntese de proteínas. A proteína de interesse, aminoglicosidase N(6')-acetiltransferase tipo 1, foi identificada e posteriormente sequenciada. Com um sinal correto, os resultados foram analisados através do Mascot e do Peaks, permitindo a identificação da proteína AAC com seis péptidos. Foi possível a identificação do péptido YSIVTNS(T) DSVTLR, que apresenta uma mutação de substituição, Asn (Aspargina) por uma Thr (Treonina). Para o péptido SHIVEWWGGEEARPTLAD VQE, apenas se obteve o match no Mascot. Como não se confirmou o match no Peaks, a classificação desta identificação foi “possível mas incerta”. Por sua vez, o péptido IA MLNGEPIGYA QSYVALGSGDGR
foi confirmado, devido a ter sido validado várias vezes, quer na sua totalidade, quer de uma só parte. Os péptidos GLGTKLVR, MSNAKTKLGITK e QAFERTRSDA não foram detetados ou sequenciados corretamente. O péptido CYEKAGFE (G) QGTVTTPYG PAVYMVQTR foi validado pelo Mascot, apresentando uma mutação na qual uma Gly (Glicina) substitui uma Arg (Arginina).
Concluiu-se desta forma que a proteómica…
Advisors/Committee Members: Igrejas, Gilberto, Santos, Hugo.
Subjects/Keywords: Escherichia coli; Resistência a antibióticos
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Magalhães, P. J. S. R. (2016). Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Magalhães, Pedro José Santana Ribeiro. “Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli.” 2016. Thesis, RCAAP. Accessed February 26, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Magalhães, Pedro José Santana Ribeiro. “Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli.” 2016. Web. 26 Feb 2021.
Vancouver:
Magalhães PJSR. Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli. [Internet] [Thesis]. RCAAP; 2016. [cited 2021 Feb 26].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Magalhães PJSR. Identificação e sequenciação da acetilase mediada pelo plasmídeo recombinante pMdT1 em Escherichia coli. [Thesis]. RCAAP; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6046
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul
22.
Vargas, Lúcia Rosane Bertholdo.
Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros.
Degree: 2008, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/15488
► As plantas possuem um arsenal de substâncias utilizadas como defesa contra patógenos e predadores. A possibilidade de utilizar tais substâncias como biopesticidas revolucionou o estudo…
(more)
▼ As plantas possuem um arsenal de substâncias utilizadas como defesa contra patógenos e predadores. A possibilidade de utilizar tais substâncias como biopesticidas revolucionou o estudo das proteínas tóxicas. Proteínas inativadoras de ribossomos (RIPs) e as ureases estão entre as proteínas que são abundantes em plantas. RIPs tipo 2 como a ricina, são muito tóxicas, e podem despurinar ribossomos de várias espécies e induzir lesão em DNA, levando à interrupção da síntese protéica e morte de células. Menos tóxicas que a ricina, a maior parte das RIPs conhecidas são do tipo 1 com apenas uma cadeia polipeptídica de 25 - 32 kDa. As ureases (EC 3.5.1.5) são metaloenzimas dependentes de níquel, que catalisam a hidrólise da uréia para formar amônia e dióxido de carbono. A semente do feijão-de-porco, Canavalia ensiformis, é fonte rica de isoformas de urease, entre elas, a canatoxina (CNTX). A proteína CNTX apresenta atividade inseticida contra diferentes espécies de insetos, e sua toxicidade depende da liberação de um peptídeo interno de 10kDa (pepcanatox), que ocorre por ação das catepsinas do sistema digestivo dos insetos suscetíveis. Um peptídeo equivalente ao pepcanatox foi obtido por expressão heteróloga em
Escherichia coli - Jaburetox-2Ec, o qual apresentou atividade tóxica contra Dysdercus peruvianus, Rhodnius prolixus e Blatella germanica. Nesse trabalho demonstramos a atividade inseticida de cinco RIPs tipo 1 (PAP-S, gelonina, momordina, saporina-S6 e licnina) em Spodoptera frugiperda e Anticarsia gemmatalis. As RIPs mostraram um efeito entomotóxico espécie-específico para as lagartas, sendo que momordina foi a menos tóxica nos bioensaios. Perda de peso mais pronunciada foi observada em S. frugiperda no 4° dia após o início dos ensaios e para a A. gemmatalis, no 10° dia. A indução da mortalidade (larval e/ou pupal) foi de 57,13% para os tratamentos em A. gemmatalis e 29,45% para S. frugiperda. Para investigar o efeito deterrente de RIPs tipo 1 em insetos, verificou-se, através do teste cometa, o nível de danos ao DNA em tecidos de S. frugiperda e A. gemmatalis que ingeriram um total de 40 μg de RIPs. Os insetos tratados com RIPs mostraram um valor 2 a 3 vezes maior de células com sinais de dano de DNA do que o controle. O dano de DNA poderia ser conseqüência do estresse oxidativo, assim analisou-se atividade de enzimas antioxidantes CAT e SOD e níveis de peroxidação lipídica (TBARS) nos extratos celulares dos insetos, mas não houve uma correlação entre dano de DNA e marcadores de estresse oxidativo. O peptídeo recombinante derivado de urease, jaburetox-2Ec, induziu uma mortalidade de 100% de S. frugiperda após ingestão de 47μg do peptídeo. Em contraste com os dados obtidos com as RIPs, o jaburetox-2Ec não causou lesões no DNA ou alterações em marcadores do balanço redox em S. frugiperda evidenciando um mecanismo de ação distinto. Em linhagens de células de insetos em cultura (Tn5B e UFL-AG-286), a análise citomorfológica sugeriu a ocorrência de citotoxicidade e lise celular com exposição a 80 e 10 μg do…
Advisors/Committee Members: Carlini, Celia Regina Ribeiro da Silva.
Subjects/Keywords: Canatoxina; Escherichia coli; Biologia molecular
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Vargas, L. R. B. (2008). Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/15488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vargas, Lúcia Rosane Bertholdo. “Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros.” 2008. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021.
http://hdl.handle.net/10183/15488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vargas, Lúcia Rosane Bertholdo. “Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros.” 2008. Web. 26 Feb 2021.
Vancouver:
Vargas LRB. Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2008. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10183/15488.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vargas LRB. Efeito inseticida de proteínas inativadoras de ribossomo tipo 1 e do Jaburetox-2Ec em lipidópteros. [Thesis]. Universidade do Rio Grande do Sul; 2008. Available from: http://hdl.handle.net/10183/15488
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul
23.
Ayres, Raquel M.
Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli.
Degree: 2014, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/129475
► As células-tronco pluripotentes induzidas (iPSCs) são as células reprogramadas com fatores de transcrição, como Oct-4, Sox-2, Klf-4 e c-MYC (OSKM). As iPSCs são geralmente obtidas…
(more)
▼ As células-tronco pluripotentes induzidas (iPSCs) são as células reprogramadas com fatores de transcrição, como Oct-4, Sox-2, Klf-4 e c-MYC (OSKM). As iPSCs são geralmente obtidas pela expressão ectópica de vetores retrovirais e lentivirais contendo os fatores transcricionais OSKM. No entanto, a utilização de sistemas de expressão virais pode levar a mutagênese de inserção e geração de células tumorais. Assim, é preferível que a reprogramação celular ocorra temporariamente por meio de fatores OSKM fusionados com sequências de peptideos para a transdução de proteínas. Neste sentido, os genes sintéticos codificadores de transdução de péptidos fusionados ao N ou C-terminal de OSKM pode ser obtido por ferramentas da bioinformática específicos e sintetizados utilizando técnicas químicas avançadas. Esses genes sintéticos podem ser aplicados para a síntese de proteínas utilizando tanto técnicas in vivo quanto in vitro. Assim, o objetivo deste trabalho foi gerar fatores OSKM fusionados a transportana (TP10) usando genes sintéticos e sistema de transcrição e de tradução in vivo. Além disso, três genes sintéticos adicionais também foram obtidos para expressão de Oct-4 contendo TP10 fusionado na região N-terminal (N-OCT4-TP10), C-terminal (C-OCT4-TP10), e sem TP10 (Oct-4). A expressão ectópica dos três genes sintéticos para Oct-4 foram testadas in vivo em duas diferentes linhagens comerciais de E. coli que expressam a enzima de T7 RNA polimerase. Os dados de Western blot indicaram que todos os genes sintéticos de Oct-4 foram capazes de produzir proteínas recombinantes. No entanto, a expressão mais elevada foi obtida com a construção N-OCT4-TP10. Além disso, as proteínas do fator de transcrição Oct-4 obtitdas foram avaliadas quanto a sua capacidade de ligação a sua respectiva seqüência-alvo de DNA sendo avaliada por meio dos extratos brutos das proteínas de E. coli contendo ou não Oct-4 proteínas ectópica. Os dados do ensaio de ligação demonstraram que todas as sequências de Oct-4 foram capazes de se ligar às suas sequências de reconhecimento. Em conclusão, as proteínas Oct-4 funcionais fusionadas com o peptideo o transdutor pode ser obtido a partir de genes sintéticos, permitindo potencialmente elaborar protocolos de reprogramação virais livres.
Induced pluripotent stem cells (iPSCs) are reprogrammed cells with a transcription factors such as Oct-4, Sox-2, Klf-4, and c-MYC (OSKM). iPSCs are generally obtained by ectopic expression of retroviral and lentiviral vectors containing tOSKM. However, the use of viral expression systems can lead to insertional mutagenesis and generation of tumor cells. Thus, it is preferable to temporarily reprogram cell by means of OSKM factors fused to peptides sequences for protein transduction. In this sense, synthetic genes coding to transduction peptides fused to the N- or C-termini of OSKM can be designed by specific bioinformatics tools and synthesized using advanced chemical techniques. Those synthetic genes can be applied for protein synthesis using both in vivo and in vitro techniques.…
Advisors/Committee Members: Bonatto, Diego.
Subjects/Keywords: Escherichia coli; Células-tronco
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ayres, R. M. (2014). Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/129475
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ayres, Raquel M. “Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021.
http://hdl.handle.net/10183/129475.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ayres, Raquel M. “Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli.” 2014. Web. 26 Feb 2021.
Vancouver:
Ayres RM. Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10183/129475.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ayres RM. Desenho e obtenção de genes sintéticos para a expressão heteróloga do fator de reprogramação celular Oct-4 fusionados a transportana 10 em diferentes linhagens de Escherichia coli. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/129475
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul
24.
Fernandes, Gabriela de Carvalho.
Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação.
Degree: 2014, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/129476
► O nitrogênio é um elemento essencial à vida na Terra. Em geral, a disponibilização desse elemento para os seres vivos se dá por meio da…
(more)
▼ O nitrogênio é um elemento essencial à vida na Terra. Em geral, a disponibilização desse elemento para os seres vivos se dá por meio da fixação biológica do nitrogênio (FBN). Os micro-organismos capazes de realizar a FBN são denominados de diazotróficos e contêm o complexo enzimático da nitrogenase. Por ser um processo extremamente dispendioso, a FBN é regulada, principalmente, em nível transcricional, em resposta à quantidade de nitrogênio fixado e aos níveis de oxigênio. Os mecanismos de regulação do processo em bactérias Gram-negativas estão bem caracterizados, porém, em bactérias Gram-positivas, os estudos ainda são escassos. Paenibacillus riograndensis é uma bactéria Gram-positiva diazotrófica aeróbia facultativa e formadora de esporos, cujo sequenciamento completo do genoma a capacita como um interessante modelo para o estudo da regulação da FBN. No genoma de P. riograndensis foram identificados três agrupamentos contendo genes relacionados à FBN. Um deles, com uma organização estrutural menos conservada, foi considerado inativo a partir de análises de PCR em tempo real e de atividade de promotor. Os outros dois tiveram seus transcritos identificados e induzidos sob condições de fixação de nitrogênio, sendo um deles responsável pela codificação de um sistema alternativo da nitrogenase, independente de molibdênio. Esse sistema alternativo foi identificado como sendo aquele composto apenas por ferro e validado tanto pela análise das sequências dos genes estruturais, como pela atividade enzimática em meio sem molibdênio. Sequências localizadas a aproximadamente 250 pares de bases (pb) a montante do início da tradução dos primeiros genes dos dois agrupamentos funcionais também tiveram suas atividades como regiões reguladoras validadas pelo reconhecimento em Escherichia coli, com um provável padrão de iniciação da transcrição constitutivo. Uma menor atividade de transcrição foi observada no fragmento de 500 pb localizado a montante do agrupamento dos genes da nitrogenase alternativa, indicando a presença de regiões contendo motivos de regulação negativa do processo. Investigações mais detalhadas dessas sequências podem revelar padrões inéditos para a regulação da FBN em bactérias Gram-positivas, em geral, e em P. riograndensis, em particular.
Nitrogen is an essential element for life. In general, it becomes available to biosphere mainly through biological nitrogen fixation (BNF). Microorganisms named diazotrophs perform BNF and they have the nitrogenase enzyme. As BNF is a very energetic expensive process, it is tightly regulated mainly at transcriptional level in response to available nitrogen and oxygen levels. Regulatory networks comprising BNF systems in Gramnegative bacteria are well characterized, while studies related to Gram-positive bacteria are scarce. Paenibacillus riograndensis is a Gram-positive endospore-forming facultative anaerobic diazotroph, whose complete genome sequence presents it as an interesting model for the study of BNF regulation. In P. riograndensis genome three cluster comprising BNF…
Advisors/Committee Members: Passaglia, Luciane Maria Pereira.
Subjects/Keywords: Paenibacillus; Nitrogen : Fixacao; Escherichia coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fernandes, G. d. C. (2014). Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/129476
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fernandes, Gabriela de Carvalho. “Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021.
http://hdl.handle.net/10183/129476.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fernandes, Gabriela de Carvalho. “Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação.” 2014. Web. 26 Feb 2021.
Vancouver:
Fernandes GdC. Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10183/129476.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fernandes GdC. Caracterização inicial do sistema genético da fixação biológica do nitrogênio em Paenibacillus riograndensis e sua regulação. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/129476
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul
25.
Teichmann, Aline.
Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.
Degree: 2010, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/30207
► O Echinococcus granulosus é um platelminto parasita da classe Cestoda, que tem, como hospedeiros definitivos, os canídeos, e, como hospedeiros intermediários mais comuns, ungulados domésticos…
(more)
▼ O Echinococcus granulosus é um platelminto parasita da classe Cestoda, que tem, como hospedeiros definitivos, os canídeos, e, como hospedeiros intermediários mais comuns, ungulados domésticos e o homem. Este parasito é o agente etiológico da hidatidose cística, zoonose hiperendêmica no Cone Sul da América do Sul, incluindo o sul do Brasil. Para o estabelecimento e manutenção do cisto hidático por longos períodos de tempo (infecção crônica), o parasito secreta e expõe ao seu hospedeiro numerosas proteínas, que podem apresentar tanto atividade imunomoduladora, como estar relacionadas a atividades fisiológicas do parasito. As proteínas 14-3-3 constituem uma família altamente conservada de moléculas reguladoras eucarióticas, que são capazes de interagir com diferentes proteínas ligantes em diversos contextos celulares, regulando funções biológicas complexas. Em E. granulosus, foram identificadas cinco proteínas 14-3-3, sendo três isoformas ζ e duas isoformas ε. Essas proteínas podem estar envolvidas em interações parasito-hospedeiro que propiciam o estabelecimento e o desenvolvimento da forma larval patogênica (metacestódeo). Para a futura caracterização funcional da proteína 14-3-3e1 de E. granulousus (Eg14-3-3ε1), já detectada em componentes do metacestódeo, a sua sequência codificadora foi expressada em Escherichia coli. A clonagem foi realizada em dois vetores plasmidiais distintos (pGEX-TEV e pET-26b), para permitir a expressão em E. coli da proteína Eg14-3-3ε1 recombinante no citoplasma, em fusão com a GST, e no espaço periplásmatico, com cauda de histidina, respectivamente. A solubilização da Eg14-3-3ε1 em fusão com a GST foi obtida com 0,5% de sarcosil, mas no entanto não foi possível a sua purificação. A proteína Eg14-3-3ε1 com cauda de histidina foi obtida por extração da fração periplasmática seguida da purificação por cromatografia de afinidade, tendo sido obtido um rendimento final de 0,5 mg/l de cultura. A proteína Eg14-3-3ε1 recombinante purificada será futuramente utilizada em ensaios funcionais, buscando inicialmente a identificação de seus ligantes proteicos dentre o repertório de proteínas do parasito e do hospedeiro, no contexto da infecção crônica.
Echinococcus granulosus is a parasitc platyhelminth of the Cestoda class, wich has, canids as definitive hosts, domestic ungulates and humans as intermediate hosts. This parasite is the causative agent of cystic hydatid disease, a hyperendemic zoonosis in the Southern Cone of South America, including Southern Brazil. In order to the establishment and maintenance of hydatid cyst for a long period of time, the parasite secrets and exposes to its host numerous proteins, which may have immunomodulatory activity as well to be related to physiological activities of the parasite. The 14-3-3 proteins are a family of highly conserved eukaryotic regulatory molecules that are able to interact with different partner proteins in different cellular contexts, regulating complex biological functions. In E. granulosus five 14-3-3 proteins have been identified, three ζ…
Advisors/Committee Members: Ferreira, Henrique Bunselmeyer.
Subjects/Keywords: Clonagem; Echinococcus granulosus; Escherichia coli
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Chicago ·
MLA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Teichmann, A. (2010). Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/30207
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Teichmann, Aline. “Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.” 2010. Thesis, Universidade do Rio Grande do Sul. Accessed February 26, 2021.
http://hdl.handle.net/10183/30207.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Teichmann, Aline. “Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli.” 2010. Web. 26 Feb 2021.
Vancouver:
Teichmann A. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2010. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10183/30207.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Teichmann A. Clonagem e expressão da proteína 14-3-3ε1 de Echinococcus granulosus em Escherichia coli. [Thesis]. Universidade do Rio Grande do Sul; 2010. Available from: http://hdl.handle.net/10183/30207
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
26.
Casella, Tiago [UNESP].
Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango.
Degree: 2016, Universidade Estadual Paulista
URL: http://hdl.handle.net/11449/144719
► A resistência a cefalosporinas de espectro estendido (ESC) mediada pela produção de β-lactamases de espectro estendido (ESBL) por Enterobacteriaceae é um problema de saúde pública…
(more)
▼ A resistência a cefalosporinas de espectro estendido (ESC) mediada pela produção de β-lactamases de espectro estendido (ESBL) por Enterobacteriaceae é um problema de saúde pública global. Neste contexto, o aumento da resistência a ESC entre
Escherichia coli isoladas de animais de produção é uma questão importante, uma vez que bactérias comensais de animais de produção podem contaminar os produtos alimentícios e chegar ao intestino humano via cadeia alimentar. O Brasil é um importante produtor e o maior exportador de carne de frango no mundo. Assim, é de extrema importância monitorar a presença dessas bactérias neste setor alimentar. No presente estudo, foi realizada uma comparação do genótipo de resistência a ESC em E.
coli isoladas a partir de carnes de frango no Brasil e na França, considerando que não há relação comercial de carne de frango entre esses dois países. Esta abordagem pode ser útil para compreender as dinâmicas dos fatores de resistência na cadeia de produção de frangos. Além disso, linhagens isoladas do trato gastrointestinal de frangos no Brasil foram também estudadas. Para isso, amostras de carnes de frango de diferentes pontos do varejo e swabs de cloaca de frangos de três diferentes fazendas foram coletadas no Estado de São Paulo, Brasil. Também, amostras de carnes de frango de diferentes pontos do varejo de Lyon, França, foram amostradas. As amostras de carne e os swabs de cloaca de frangos foram inoculados em ágar MacConkey acrescido com ESC. As colônias foram identificadas por sistema automatizado, e a suscetibilidade aos antimicrobianos foi testada por disco-difusão. PCR para detecção de genes blaESBL e blapAmpC e para a determinação dos grupos filogenéticos de E.
coli foram realizadas. Os genes bla foram sequenciados e os plasmídeos foram caracterizados pelo esquema PBRT e por southern blot/hibridação de géis de PFGE-S1. Quase todas as carnes de frango apresentaram, ao menos, uma linhagem de E.
coli resistente a ESC, e 53,8% dos frangos analisados também estavam colonizados por E.
coli resistente a ESC. Resistência a aminoglicosídeos, fenicóis, quinolonas, sulfonamidas, tetraciclina e trimetoprim também foi detectada. Um total de 60 linhagens isoladas de amostras do Brasil foram estudadas, e o gene blaCTX-M-2 foi o principal responsável pela resistência a ESC, mas blaCTX-M-55, blaCMY-2, blaCTX-M-15 e blaCTX-M-8 também foram detectados. De 77 linhagens isoladas de amostras de carne de frango da França, o gene blaCTX-M-1 foi o principal responsável pela resistência a ESC, mas blaTEM-52, blaCMY-2 e blaSHV-12 também foram detectados. A maioria dos blaCTX-M-2 estão associados à ISCR1 e a integrons complexos de classe 1, e blaCTX-M-1, blaCTX-M-15, blaCTX-M-55, e os oito blaCMY-2 do Brasil estão precedidos pela ISEcp1. O gene blaCTX-M-8 está associado à IS10. Nas amostras do Brasil, um gene blaCTX-M-2 é carreado por um plasmídeo IncHI2/P, blaCTX-M-8 por um plasmídeo IncI1, um gene blaCTX-M-15 por um plasmídeo IncX1, e os genes blaCTX-M-55 por plasmídeos IncFII ou IncN/FII. Nas amostras de carnes de…
Advisors/Committee Members: Nogueira, Mara Corrêa Lelles [UNESP], Gutkind, Gabriel Osvaldo, Madec, Jean-Yves, Universidade Estadual Paulista (UNESP).
Subjects/Keywords: Escherichia coli; ESBL; pAmpC; Frango
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Casella, T. [. (2016). Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/144719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Casella, Tiago [UNESP]. “Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango.” 2016. Thesis, Universidade Estadual Paulista. Accessed February 26, 2021.
http://hdl.handle.net/11449/144719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Casella, Tiago [UNESP]. “Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango.” 2016. Web. 26 Feb 2021.
Vancouver:
Casella T[. Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango. [Internet] [Thesis]. Universidade Estadual Paulista; 2016. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/11449/144719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Casella T[. Caracterização molecular de genes de resistência a cefalosporinas de espectro estendido em Escherichia coli isoladas de frango e carnes de frango. [Thesis]. Universidade Estadual Paulista; 2016. Available from: http://hdl.handle.net/11449/144719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University
27.
Wilson, Jessica Grissett.
Investigation of the beneficial effect of <i>Enterobacter cloacae</i> strain JD6301 on mice challenged with <i>Escherichia coli</i> O157:H7.
Degree: MS, Biological Sciences, 2014, Mississippi State University
URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/
;
► The environment of the gastrointestinal (GI) tract is an extremely complex system made up of not only host cells, but also many beneficial microbes.…
(more)
▼ The environment of the gastrointestinal (GI) tract is an extremely complex system made up of not only host cells, but also many beneficial microbes. Disruption of this environment can often lead to disorders and health issues. To help balance this system, probiotics have often been administered to both humans and animals, such as livestock. This study aimed to determine what beneficial effects a novel strain of <i>Enterobacter cloacae</i>, strain JD6301, could offer to a host in the presence of an enteric infection with <i>E.
coli</i> O157:H7. Upon administration of JD6301, supplemented animals had overall less <i>E.
coli</i> present in the colon and caecum. Moreover, these animals shed more <i>E.
coli</i> than control groups. Supplemented animals also had increased concentrations of serum triglycerides one day prior to challenge. Together, these data suggest that <i>Enterobacter cloacae</i> JD6301 could perform as a novel probiotic providing energy and protection to the host.
Advisors/Committee Members: Janet R Donaldson (chair), Jeffery A Carroll (committee member), James A Stewart Jr. (committee member), Justin A Thornton (committee member).
Subjects/Keywords: Escherichia coli; probiotic; Enterobacter cloacae
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wilson, J. G. (2014). Investigation of the beneficial effect of <i>Enterobacter cloacae</i> strain JD6301 on mice challenged with <i>Escherichia coli</i> O157:H7. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;
Chicago Manual of Style (16th Edition):
Wilson, Jessica Grissett. “Investigation of the beneficial effect of <i>Enterobacter cloacae</i> strain JD6301 on mice challenged with <i>Escherichia coli</i> O157:H7.” 2014. Masters Thesis, Mississippi State University. Accessed February 26, 2021.
http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;.
MLA Handbook (7th Edition):
Wilson, Jessica Grissett. “Investigation of the beneficial effect of <i>Enterobacter cloacae</i> strain JD6301 on mice challenged with <i>Escherichia coli</i> O157:H7.” 2014. Web. 26 Feb 2021.
Vancouver:
Wilson JG. Investigation of the beneficial effect of <i>Enterobacter cloacae</i> strain JD6301 on mice challenged with <i>Escherichia coli</i> O157:H7. [Internet] [Masters thesis]. Mississippi State University; 2014. [cited 2021 Feb 26].
Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;.
Council of Science Editors:
Wilson JG. Investigation of the beneficial effect of <i>Enterobacter cloacae</i> strain JD6301 on mice challenged with <i>Escherichia coli</i> O157:H7. [Masters Thesis]. Mississippi State University; 2014. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10312014-111145/ ;

University of Alberta
28.
Laidlaw, Anna M.
Control and Detection of Enterohaemorrhagic Escherichia
coli.
Degree: PhD, Department of Agricultural, Food, and Nutritional
Science, 2016, University of Alberta
URL: https://era.library.ualberta.ca/files/c9z902z880
► Enterohaemorrhagic Escherichia coli (EHEC) is a pathogen that causes severe disease in humans and has a low infectious dose. Since foodborne EHEC outbreaks continue to…
(more)
▼ Enterohaemorrhagic Escherichia coli (EHEC) is a
pathogen that causes severe disease in humans and has a low
infectious dose. Since foodborne EHEC outbreaks continue to be a
problem worldwide, improved control and detection methods for EHEC
on at-risk foods, such as spinach and beef, are essential. While
new methods of control and detection may prove to be effective in
broth or buffer, it is important that they are also tested in a
food matrix since food systems are more complex and may lead to
different results. A novel intervention method was developed to
control EHEC on spinach and lettuce with a volatile antimicrobial
from mustard, allyl isothiocyanate (AITC). AITC released from its
precursor sinigrin in mustard meal was limited by the activity of
mustard’s endogenous enzyme myrosinase at 4°C. While the
requirement of endogenous myrosinase in mustard meal to catalyze
this reaction was known, decreased activity at refrigeration
temperature was not, and made this antimicrobial intervention on
produce impractical. Secondly, this work explored improving EHEC
detection methods. Enrichment remains a necessary but lengthy step
in pathogen detection methods and a major impediment to rapid
detection. Therefore, decreases in the current validated enrichment
times for EHEC detection were investigated with aim to reduce
pathogen detection times. Individual lag phase of heat injured E.
coli O157:H7 cells were measured to determine necessary enrichment
times. However, decreasing enrichment times increased the
probability of not detecting sub-lethally injured cells since
sub-lethal cell injury significantly increased lag phases and
therefore overall time to detection. Lastly, a major challenge of
qPCR detection is the inability to discriminate between live and
dead cells within a sample and this limitation could lead to false
positive result. This is especially of importance when using this
method for pathogen detection in food. The current work
investigated five different detection methods to determine the
concentration of viable EHEC cells on beef steaks after
interventions of lactic acid, peroxyacetic acid and hot water and
included 1) use of a DNA binding dye propidium monoazide (PMA) to
prevent dead cell DNA amplification when used in conjunction with
qPCR 2) PMA in addition to membrane emulsifying deoxycholate
treatment to increase penetration of the dye and therefore increase
accuracy of viable cell DNA amplification with qPCR quantification
3) mRNA and 4) rRNA qPCR quantification and 5) conventional
plating. Treatment of samples with PMA and deoxycholate was
reported in the literature to be successful in broth in preventing
dead cell amplification in qPCR and within this research, the same
treatment used within a food system confirmed these findings;
however, it proved to be more complicated than in broth. While the
combination treatment of PMA and deoxycholate prevented
amplification of all DNA from dead cells, it also killed the
sub-lethally injured cell population within samples and
subsequently this rendered their…
Subjects/Keywords: Enterohaemorrhagic Escherichia coli; Detection; Control
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Laidlaw, A. M. (2016). Control and Detection of Enterohaemorrhagic Escherichia
coli. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/c9z902z880
Chicago Manual of Style (16th Edition):
Laidlaw, Anna M. “Control and Detection of Enterohaemorrhagic Escherichia
coli.” 2016. Doctoral Dissertation, University of Alberta. Accessed February 26, 2021.
https://era.library.ualberta.ca/files/c9z902z880.
MLA Handbook (7th Edition):
Laidlaw, Anna M. “Control and Detection of Enterohaemorrhagic Escherichia
coli.” 2016. Web. 26 Feb 2021.
Vancouver:
Laidlaw AM. Control and Detection of Enterohaemorrhagic Escherichia
coli. [Internet] [Doctoral dissertation]. University of Alberta; 2016. [cited 2021 Feb 26].
Available from: https://era.library.ualberta.ca/files/c9z902z880.
Council of Science Editors:
Laidlaw AM. Control and Detection of Enterohaemorrhagic Escherichia
coli. [Doctoral Dissertation]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/c9z902z880

Oregon State University
29.
Couch, David Bruce.
The effect of streptomycin on proline synthesis in Escherichia coli.
Degree: MS, Biochemistry and Biophysics, 1967, Oregon State University
URL: http://hdl.handle.net/1957/47094
► The effect of streptomycin on an early reaction of proline synthesis, the production of glutamic-γ-semialdehyde, in resting cell suspensions of antibiotic-sensitive, resistant, and dependent strains…
(more)
▼ The effect of streptomycin on an early reaction of proline
synthesis, the production of glutamic-γ-semialdehyde, in resting
cell suspensions of antibiotic-sensitive, resistant, and dependent
strains of
Escherichia coli was examined, and the effect on glutamic-γ-semialdehyde production was found to correspond to the growth
response of the organisms to streptomycin. Inhibition of glutamic-γ-semialdehyde production in sensitive strains was found not to be
caused by streptomycin-induced changes in permeability to substrate
or product of the reaction. The effects of pH, ionic strength, and
the presence of purines, pyrimidines, and nucleotides on streptomycin
action were examined, and possibilities for the mode and site of
streptomycin action are discussed.
Advisors/Committee Members: Baich, Annette (advisor).
Subjects/Keywords: Escherichia coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Couch, D. B. (1967). The effect of streptomycin on proline synthesis in Escherichia coli. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47094
Chicago Manual of Style (16th Edition):
Couch, David Bruce. “The effect of streptomycin on proline synthesis in Escherichia coli.” 1967. Masters Thesis, Oregon State University. Accessed February 26, 2021.
http://hdl.handle.net/1957/47094.
MLA Handbook (7th Edition):
Couch, David Bruce. “The effect of streptomycin on proline synthesis in Escherichia coli.” 1967. Web. 26 Feb 2021.
Vancouver:
Couch DB. The effect of streptomycin on proline synthesis in Escherichia coli. [Internet] [Masters thesis]. Oregon State University; 1967. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1957/47094.
Council of Science Editors:
Couch DB. The effect of streptomycin on proline synthesis in Escherichia coli. [Masters Thesis]. Oregon State University; 1967. Available from: http://hdl.handle.net/1957/47094

Oregon State University
30.
Moreno, Daniel.
Migration of E. coli and solutes to tile drains via preferential and matrix flow.
Degree: MS, Soil Science, 2002, Oregon State University
URL: http://hdl.handle.net/1957/29676
► The extent of agricultural drainage has created concern for its potential undesirable effects on surface water quality. Land applications of liquid manure on tile drain…
(more)
▼ The extent of agricultural drainage has created concern for its potential undesirable
effects on surface water quality. Land applications of liquid manure on tile drain
fields have the potential to transport solutes and bacteria to the drains following
precipitation or irrigation events and many times are directly sent to a surface water
body, and have been documented as a source of contamination of surface waters.
This study determined the potential for and magnitude of E.
coli and solute
migration to tile drains through the soil profile. Water from subsurface drains was
analyzed for chemical and bacterial composition following tracer applications.
Two sites were selected for the study to determine transport at large (field) and
small (plot) scales. At the large-scale site, both tracers, bacteria (E.
coli and Total
Coliform) and Amino-G (a conservative tracer), were used to monitor the speed of
transport from the surface to the tile drain following liquid manure applications,
tracer applications and additionally precipitation events. The concentrations of E.
coli were monitored every hour for 76 days during the spring. Both tracers,
bacteria and Amino-G, were detected in the tile drainage shortly after precipitation
events. The peak concentration of E.
coli was observed to be 1.2 x 10⁶
CFU/l00mL. These elevated concentrations of E.
coli might be attributed to the
characteristics of the soil, high organic matter and well-structured clay soils. Both
the rapid breakthrough of tracer to the tile drain and the peaks of tile water
temperature during precipitation events provided evidence of macropore flow.
Antecedent soil moisture and warmer temperatures appeared to provide ideal
conditions for bacteria growth.
The small-scale study site was selected for a more focused study. The purpose of
this site was to quantify more accurately the percent mass of surface applied tracer
that was transported to the tile drain, allowing mass balance calculations.
Experiments were conducted during the summer to control the rate and total
amount of irrigation. Amino-G readings were taken every 10 seconds for 125
hours of continuous irrigation. Tracer applications were conducted at runoff and
non-runoff conditions. Both types of tracer applications had Amino-G
breakthrough in less than 10 minutes after initiation of irrigation. Tracer applied at
runoff rates resulted in 4 to 17 times more total tracer mass migrating to the tile
drain than when applied at non-runoff rates. The total mass of Amino-G migrating
to the tile drain during non-runoff conditions depended on the total volume of
applied tracer, regardless of the tracer concentration. For an application of 5.6 mm
at 12 mg/L, 5.7% of the total applied tracer migrated to the tile drain, whereas for
an application of 1.9 mm at 27.7 mg/L only 2.8% of the total applied tracer
migrated to the tile drain. Tile flow response to irrigation experiments appeared to
be governed by soil moisture. Lysimeter samples were taken continuously every 4-8 hours until…
Advisors/Committee Members: Dragila, Maria I. (advisor), Buckhouse, John (committee member).
Subjects/Keywords: Escherichia coli
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Moreno, D. (2002). Migration of E. coli and solutes to tile drains via preferential and matrix flow. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/29676
Chicago Manual of Style (16th Edition):
Moreno, Daniel. “Migration of E. coli and solutes to tile drains via preferential and matrix flow.” 2002. Masters Thesis, Oregon State University. Accessed February 26, 2021.
http://hdl.handle.net/1957/29676.
MLA Handbook (7th Edition):
Moreno, Daniel. “Migration of E. coli and solutes to tile drains via preferential and matrix flow.” 2002. Web. 26 Feb 2021.
Vancouver:
Moreno D. Migration of E. coli and solutes to tile drains via preferential and matrix flow. [Internet] [Masters thesis]. Oregon State University; 2002. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1957/29676.
Council of Science Editors:
Moreno D. Migration of E. coli and solutes to tile drains via preferential and matrix flow. [Masters Thesis]. Oregon State University; 2002. Available from: http://hdl.handle.net/1957/29676
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