You searched for subject:(Epithelial cells).
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724 total matches.
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University of California – Merced
1.
Pettengill, Matthew Aaron.
Danger signals and the regulation of Chlamydia infection in epithelial cells.
Degree: Quantitative and Systems Biology, 2010, University of California – Merced
URL: http://www.escholarship.org/uc/item/3w77g2qc 
► Bacterial infections of the mucosal epithelium initiate inflammation of the local tissues, which leads to changes in the molecular profile of the extracellular milieu, including…
(more)
▼ Bacterial infections of the mucosal epithelium initiate inflammation of the local tissues, which leads to
changes in the molecular profile of the extracellular milieu, including increases in extracellular ATP, and
its ecto-enzymatic and hydrolytic degradation products ADP and adenosine. Extracellular ATP and
adenosine function as signaling molecules at purinergic receptors which have been shown to regulate
several key aspects of immune cell function. Information has been quite limited, however, on how this
may alter bacterial growth in the inflamed tissue. Here we demonstrate that stimulation of Chlamydia
trachomatis infected epithelial cells with micromolar extracellular ATP, ADP and adenosine significantly
impairs growth of the bacteria. The response to adenosine is mediated by the A2b receptor, while the
effects of ATP and ADP suggest involvement of P2X4 receptors. Chlamydial growth is reversibly
inhibited, and the bacteria return to normal growth kinetics following removal of the stimulus. This
suggests that extracellular adenosine and adenine nucleotides are important for local control of C.
trachomatis at infected mucosal epithelium, and that further characterization of the intracellular
mechanisms employed by the host could lead to improved methods of treating this important pathogen.
Subjects/Keywords: Biology.; Chlamydia.; Epithelial cells.
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APA (6th Edition):
Pettengill, M. A. (2010). Danger signals and the regulation of Chlamydia infection in epithelial cells. (Thesis). University of California – Merced. Retrieved from http://www.escholarship.org/uc/item/3w77g2qc
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pettengill, Matthew Aaron. “Danger signals and the regulation of Chlamydia infection in epithelial cells.” 2010. Thesis, University of California – Merced. Accessed March 04, 2021.
http://www.escholarship.org/uc/item/3w77g2qc.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pettengill, Matthew Aaron. “Danger signals and the regulation of Chlamydia infection in epithelial cells.” 2010. Web. 04 Mar 2021.
Vancouver:
Pettengill MA. Danger signals and the regulation of Chlamydia infection in epithelial cells. [Internet] [Thesis]. University of California – Merced; 2010. [cited 2021 Mar 04].
Available from: http://www.escholarship.org/uc/item/3w77g2qc.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pettengill MA. Danger signals and the regulation of Chlamydia infection in epithelial cells. [Thesis]. University of California – Merced; 2010. Available from: http://www.escholarship.org/uc/item/3w77g2qc
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Waterloo
2.
Li, Ziqing.
Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells.
Degree: 2019, University of Waterloo
URL: http://hdl.handle.net/10012/14997
► Crystallins are proteins that confer refractive properties to the crystalline lens. All vertebrate lenses contain α- and β-crystallins, and often a third major crystallin. Crystallins…
(more)
▼ Crystallins are proteins that confer refractive properties to the crystalline lens. All vertebrate lenses contain α- and β-crystallins, and often a third major crystallin. Crystallins can have additional non-refractive functions; α-crystallins act as heat shock proteins, protecting lenses from heat-induced denaturation, and γ-crystallins are thought to be cryoproteins, protecting lenses from extreme cold. The concentration of α-crystallins is higher in mammalian lenses than in teleost lenses, while the opposite is true for γ-crystallins, suggesting that mammalian lenses would be better protected in warmer conditions and teleost lenses better protected in colder temperatures. This study determined whether temperature affects the growth of lens epithelial cells (LECs) derived from human and fish lenses. Both human and rainbow trout fish LECs were cultured (n = 4 each) and grown for 1, 2, 4, 6, 8 and 12 days at the optimal (37°C and 18°C, respectively), higher than optimal (42°C and 25°C, respectively) and lower than optimal temperatures (32°C and 10°C, respectively). At optimal temperatures, both fish and human LECs grew optimally. Higher temperatures were more deleterious to the proliferation index than lower temperatures for both human and fish LECs. Mitotic cells were non-existent in fish LECs grown at high temperatures. The sizes of the cells did not greatly change with temperature with either species, but human cells at non-optimal temperature tended to clump over time. Human LECs at the optimal temperature maintained their random distribution. Fish LECs at optimal temperatures moved from a random distribution to a clumped distribution, but lower temperatures had the opposite effect; LECs moved from a clumped to a random distribution. Only the high temperature group of fish LECs maintain their random organisation.
Subjects/Keywords: lens epithelial cells; crystallin; temperature
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APA (6th Edition):
Li, Z. (2019). Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/14997
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Ziqing. “Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells.” 2019. Thesis, University of Waterloo. Accessed March 04, 2021.
http://hdl.handle.net/10012/14997.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Ziqing. “Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells.” 2019. Web. 04 Mar 2021.
Vancouver:
Li Z. Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells. [Internet] [Thesis]. University of Waterloo; 2019. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10012/14997.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li Z. Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells. [Thesis]. University of Waterloo; 2019. Available from: http://hdl.handle.net/10012/14997
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manitoba
3.
Kroeker, Andrea.
A proteomic approach to discovering novel anti-influenza mechanisms in primary human airway epithelial cells.
Degree: Physiology and Pathophysiology, 2012, University of Manitoba
URL: http://hdl.handle.net/1993/30400
► The influenza virus has a large impact on global health; however, it is difficult to formulate vaccines and influenza therapies that are effective against influenza.…
(more)
▼ The influenza virus has a large impact on global health; however, it is difficult to formulate vaccines and influenza therapies that are effective against influenza. The influenza virus mutates rapidly, has the ability to emerge as novel strains with pandemic potential and can quickly become resistant to any given drug. Therefore, the generation of novel anti-influenza therapeutics that are effective against multiple strains would be highly beneficial. To date, the majority of anti-influenza research has focused on targeting specific components of the virus in order to interfere with its replication. However, it has been proposed that host proteins and signaling pathways may be essential components to viral replication and could also become novel anti-influenza drug targets. Therefore, this study utilized a large proteomic screen to identify host proteins that were up- and down-regulated in response to influenza infection. Collectively, these proteins clustered into five specific cell pathways and processes including interferon signaling, purine metabolism, cell death, ubiquitin-like signaling and mitochondrial oxidoreductases. Overall, this project identified potential novel anti-influenza targets in primary airway
epithelial cells.
Advisors/Committee Members: Halayko, Andrew (Physiology and Pathophysiology) Coombs, Kevin (Medical Microbiology) (supervisor), Nickerson, Peter (Immunology) Czybryt, Michael (Physiology and Pathophysiology) Dodd, Janice (Physiology and Pathophysiology) Mossman, Karen (McMaster University) (examiningcommittee).
Subjects/Keywords: influenza; proteomics; airway epithelial cells
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APA (6th Edition):
Kroeker, A. (2012). A proteomic approach to discovering novel anti-influenza mechanisms in primary human airway epithelial cells. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/30400
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kroeker, Andrea. “A proteomic approach to discovering novel anti-influenza mechanisms in primary human airway epithelial cells.” 2012. Thesis, University of Manitoba. Accessed March 04, 2021.
http://hdl.handle.net/1993/30400.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kroeker, Andrea. “A proteomic approach to discovering novel anti-influenza mechanisms in primary human airway epithelial cells.” 2012. Web. 04 Mar 2021.
Vancouver:
Kroeker A. A proteomic approach to discovering novel anti-influenza mechanisms in primary human airway epithelial cells. [Internet] [Thesis]. University of Manitoba; 2012. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1993/30400.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kroeker A. A proteomic approach to discovering novel anti-influenza mechanisms in primary human airway epithelial cells. [Thesis]. University of Manitoba; 2012. Available from: http://hdl.handle.net/1993/30400
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of North Carolina – Greensboro
4.
Covell, Alan D.
At the interface: biotic-abiotic interactions between
substrates and a model epithelium.
Degree: 2015, University of North Carolina – Greensboro
URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887
► The need for determining the fundamental mechanisms that define the interaction of biological systems with underlying materials, both natural and synthetic, is important as humanity…
(more)
▼ The need for determining the fundamental mechanisms
that define the interaction of biological systems with underlying
materials, both natural and synthetic, is important as humanity
endeavors to improve the quality of life of individuals through
technology. Recently, much work has focused on the role of material
properties on the behavior of
cells. Most of these studies have
concentrated their efforts on fibroblastic cell lines and more
recently different kinds of stems
cells. While these
cells
represent an important subset of
cells in complex organisms, they
do not manifest cell-cell interactions, a feature of
epithelial
cells, the most abundant cell type.
Epithelial cells represent the
largest cell type in the body and introduce an intrinsic complexity
when researching the interaction of biological systems with
materials. Adherens junctions (AJ) play a significant role in many
signaling pathways, and therefore there is need to investigate how
physical interactions with underlying substrates affect cell-cell
interactions, such as the adhesion properties between
cells, as
well as how cell-substrate interactions influence the morphology
and growth of
epithelial cells. In this work I seek to determine
the effects and identify mechanisms that
epithelial cells use to
“read” their environment. To do this I examined changes in cell
behavior (growth, morphological, adhesion) of a model epithelium on
substrates that have similar composition but significant
differences in surface organization. In such a manner, I probed the
limitations at which the nanoscale differences in substrate
topography affect cellular behavior.;
Epithelial Cell, MDCK,
Nanotopography
Advisors/Committee Members: Dennis LaJeunesse (advisor).
Subjects/Keywords: Epithelial cells; Cell interaction; Nanotechnology
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Manager
APA (6th Edition):
Covell, A. D. (2015). At the interface: biotic-abiotic interactions between
substrates and a model epithelium. (Doctoral Dissertation). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887
Chicago Manual of Style (16th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between
substrates and a model epithelium.” 2015. Doctoral Dissertation, University of North Carolina – Greensboro. Accessed March 04, 2021.
http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887.
MLA Handbook (7th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between
substrates and a model epithelium.” 2015. Web. 04 Mar 2021.
Vancouver:
Covell AD. At the interface: biotic-abiotic interactions between
substrates and a model epithelium. [Internet] [Doctoral dissertation]. University of North Carolina – Greensboro; 2015. [cited 2021 Mar 04].
Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887.
Council of Science Editors:
Covell AD. At the interface: biotic-abiotic interactions between
substrates and a model epithelium. [Doctoral Dissertation]. University of North Carolina – Greensboro; 2015. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887
McGill University
5.
MacLeod, R. John.
Signal Transduction of Volume Regulation in Villus Epithelial Cells.
Degree: PhD, Division of Experimental Medicine, 1996, McGill University
URL: https://escholarship.mcgill.ca/downloads/9019s4836.pdf
;
https://escholarship.mcgill.ca/concern/theses/bz60cz954
► Des augmentations plus ou moins importantes du volume des cellules épithéliales du jéjunum, actives dans l'absorption du Na+, entrainent l'activation des canaux K+ et er·,…
(more)
▼ Des augmentations plus ou moins importantes du volume des cellules épithéliales du jéjunum, actives dans l'absorption du Na+, entrainent l'activation des canaux K+ et er·, le flux des ions K+ et er· qui en résulte amené une diminution régulatrice de volume (DRV) qui rétablit le volume normal de la cellule. L'objectif de la présente thèse est de montrer à quel point I' importance de I' augmentation de volume est un facteur déterminant de l'activation des canaux K+ responsables de la DRV des cellules des villosités jéjunales chez le cochon d'inde. Nous avons déterminé s'il existait un lien entre les variations du pH intracellulaire (pHi) et la DRV après de faibles augmentations de volume (5 à 7 %), qui reproduisaient le gonflement cellulaire durant l'absorption du Na+ en solution, comparativement à des augmentations plus importantes de volume observées après des dilutions hypotoniques standard (dilutions de la solution isotonique de 0.7 à 0.5 fois). […]
Volume increases of any magnitude in Na+ -absorbing jejunal epithelial cells result in the activation of K+ and et· channels; the resultant é and et· efflux causes a Regulatory Volume Decrease (RVD) restoring cell volume to normal. The objective of this thesis was to determine how the extent of volume increase was a determinant of the signaling of the K+ channels responsible for RVD in guinea pig jejunal villus cells. We determined whether changes in intracellular pH (pHi) were related to RVD after modest 5 to 7% volume increases, which duplicated the extent of cell swelling during Na+ -solute absorption, compared with larger volume increases observed after "standard" hypotonic (0. 7 to 0.5 x isotonic) dilutions. Cell volume determinations were made using electronic cell sizing and pHi was measured by fluorescent spectroscopy of villus cells in suspension. […]
Advisors/Committee Members: Hamilton, Dick (Supervisor).
Subjects/Keywords: Epithelial cells
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APA ·
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Export
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Manager
APA (6th Edition):
MacLeod, R. J. (1996). Signal Transduction of Volume Regulation in Villus Epithelial Cells. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/9019s4836.pdf ; https://escholarship.mcgill.ca/concern/theses/bz60cz954
Chicago Manual of Style (16th Edition):
MacLeod, R John. “Signal Transduction of Volume Regulation in Villus Epithelial Cells.” 1996. Doctoral Dissertation, McGill University. Accessed March 04, 2021.
https://escholarship.mcgill.ca/downloads/9019s4836.pdf ; https://escholarship.mcgill.ca/concern/theses/bz60cz954.
MLA Handbook (7th Edition):
MacLeod, R John. “Signal Transduction of Volume Regulation in Villus Epithelial Cells.” 1996. Web. 04 Mar 2021.
Vancouver:
MacLeod RJ. Signal Transduction of Volume Regulation in Villus Epithelial Cells. [Internet] [Doctoral dissertation]. McGill University; 1996. [cited 2021 Mar 04].
Available from: https://escholarship.mcgill.ca/downloads/9019s4836.pdf ; https://escholarship.mcgill.ca/concern/theses/bz60cz954.
Council of Science Editors:
MacLeod RJ. Signal Transduction of Volume Regulation in Villus Epithelial Cells. [Doctoral Dissertation]. McGill University; 1996. Available from: https://escholarship.mcgill.ca/downloads/9019s4836.pdf ; https://escholarship.mcgill.ca/concern/theses/bz60cz954
6.
Covell, Alan D.
At the interface: biotic-abiotic interactions between substrates and a model epithelium.
Degree: 2015, NC Docks
URL: http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf
► The need for determining the fundamental mechanisms that define the interaction of biological systems with underlying materials, both natural and synthetic, is important as humanity…
(more)
▼ The need for determining the fundamental mechanisms that define the interaction of biological systems with underlying materials, both natural and synthetic, is important as humanity endeavors to improve the quality of life of individuals through technology. Recently, much work has focused on the role of material properties on the behavior of cells. Most of these studies have concentrated their efforts on fibroblastic cell lines and more recently different kinds of stems cells. While these cells represent an important subset of cells in complex organisms, they do not manifest cell-cell interactions, a feature of epithelial cells, the most abundant cell type. Epithelial cells represent the largest cell type in the body and introduce an intrinsic complexity when researching the interaction of biological systems with materials. Adherens junctions (AJ) play a significant role in many signaling pathways, and therefore there is need to investigate how physical interactions with underlying substrates affect cell-cell interactions, such as the adhesion properties between cells, as well as how cell-substrate interactions influence the morphology and growth of epithelial cells. In this work I seek to determine the effects and identify mechanisms that epithelial cells use to “read” their environment. To do this I examined changes in cell behavior (growth, morphological, adhesion) of a model epithelium on substrates that have similar composition but significant differences in surface organization. In such a manner, I probed the limitations at which the nanoscale differences in substrate topography affect cellular behavior.
Subjects/Keywords: Epithelial cells; Cell interaction; Nanotechnology
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❌
APA ·
Chicago ·
MLA ·
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CSE |
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Manager
APA (6th Edition):
Covell, A. D. (2015). At the interface: biotic-abiotic interactions between substrates and a model epithelium. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between substrates and a model epithelium.” 2015. Thesis, NC Docks. Accessed March 04, 2021.
http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between substrates and a model epithelium.” 2015. Web. 04 Mar 2021.
Vancouver:
Covell AD. At the interface: biotic-abiotic interactions between substrates and a model epithelium. [Internet] [Thesis]. NC Docks; 2015. [cited 2021 Mar 04].
Available from: http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Covell AD. At the interface: biotic-abiotic interactions between substrates and a model epithelium. [Thesis]. NC Docks; 2015. Available from: http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Aberdeen
7.
Sagga, Nada A.
Dynamics of limbal and conjunctival stem cell activity during ocular surface maintenance.
Degree: PhD, 2017, University of Aberdeen
URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153235090005941
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731630
► Corneal degenerative diseases and opacity are leading causes of corneal impairment and blindness worldwide. Like many epithelial tissues, the constant renewal of transparent corneal epithelial…
(more)
▼ Corneal degenerative diseases and opacity are leading causes of corneal impairment and blindness worldwide. Like many epithelial tissues, the constant renewal of transparent corneal epithelial cells is essential for a lifelong healthy cornea and optimal vision. The limbus (the boundary between the cornea and the conjunctiva) is believed to be the site that harbours adult stem cells responsible for corneal maintenance and repair after injury, referred to as limbal epithelial stem cells (LESCs). In the basal limbal epithelium, an active LESC subset divides to yield progenitor cells that migrate centripetally into the corneal epithelium for cell renewal. This asymmetric division however, is assumed to be regulated by a balance between cell renewal and loss of cells from the corneal surface. The search for specific LESC molecular markers has been difficult and to date there are few if any candidate markers that unambiguously identify LESCs but not their immediate progeny. Consequently, LESC clonality, activity and proliferative dynamics have remained poorly understood. In addition, the nature of the regulatory molecular pathways involved during LESC activity is still an open key question. In this research project, we identified stem cells on the ocular surface of the eye, assayed their activity and demonstrated quantitively for the first time how the cornea responds to damage. The retention of DNA labelling reagents in control and wounded corneas was combined with clonal analyses of cell division and migration using mice mosaic for reporter LacZ expression. Corneal transplant in LacZ reporter transgenic mice showed migration of LacZ-positive cells into the donor corneal button, with long-term disruption of patterns of migration. Corneal epithelial scrape wounds at the periphery also showed long– term disruption. Label retention suggested a small but statistically significant proliferation response of LESCs to injury, but this was attenuated or absent in aging mice and Pax6 mutants. The Hippo signalling pathway has been shown to have promising results in regulating stem cell activity and proliferation in many organs, however, its effect on LESC proliferation is unknown. Here, we investigated the regulatory role of the Hippo−YAP signalling pathway during cell proliferation, and determined whether the label retention assay in a uniform population of dividing cells is a real indicator of slow-cycling cells in vivo. Cell-cycling kinetics, Abstract v proliferation rate, and label retention were determined in a spontaneous human corneal epithelial (HCE-S) cell line, using double-labelling IdU and EdU thymidine analogues. During homeostasis, HCE-S cells underwent approximately one cell cycle per day, however, cells in which YAP-dependent signalling was activated by 17-Allylamino-17-demethoxygeldanamycin (17-AAG), an inhibitor of heat shock protein 90 (Hsp90), showed slower proliferation rate and longer cell-cycle time. In vitro label-retention assay in confluent cultures estimated number of ~3-4 cell cycles needed to dilute out…
Subjects/Keywords: 610; Cornea; Epithelial cells; Stem cells
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APA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sagga, N. A. (2017). Dynamics of limbal and conjunctival stem cell activity during ocular surface maintenance. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153235090005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731630
Chicago Manual of Style (16th Edition):
Sagga, Nada A. “Dynamics of limbal and conjunctival stem cell activity during ocular surface maintenance.” 2017. Doctoral Dissertation, University of Aberdeen. Accessed March 04, 2021.
https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153235090005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731630.
MLA Handbook (7th Edition):
Sagga, Nada A. “Dynamics of limbal and conjunctival stem cell activity during ocular surface maintenance.” 2017. Web. 04 Mar 2021.
Vancouver:
Sagga NA. Dynamics of limbal and conjunctival stem cell activity during ocular surface maintenance. [Internet] [Doctoral dissertation]. University of Aberdeen; 2017. [cited 2021 Mar 04].
Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153235090005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731630.
Council of Science Editors:
Sagga NA. Dynamics of limbal and conjunctival stem cell activity during ocular surface maintenance. [Doctoral Dissertation]. University of Aberdeen; 2017. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153235090005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731630
University of Bath
8.
Doughton, Gail Louise.
Cell fate specification and polarisation in mouse preimplantation epithelia.
Degree: PhD, 2014, University of Bath
URL: https://researchportal.bath.ac.uk/en/studentthesis/cell-fate-specification-and-polarisation-in-mouse-preimplantation-epithelia(d8fbae5b-285b-4413-b370-3aa32ad4d607).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619153
► Understanding the establishment of polarity and the cell fate specification of epithelial cells is important for developmental biology, regenerative medicine and the study of cancer.…
(more)
▼ Understanding the establishment of polarity and the cell fate specification of epithelial cells is important for developmental biology, regenerative medicine and the study of cancer. In this thesis, models of pre-implantation epithelial development are used to investigate the relationship between these two processes. The trophoblast is an extraembryonic epithelial tissue which contributes to the placenta. Addition of BMP4 to mouse and human embryonic stem (mES) cells grown in culture has been suggested to induce differentiation of cells to the trophoblast lineage. The use of this differentiation method was investigated as a possible model of trophoblast polarisation and cell fate specification. Unfortunately, with the protocol and reagents available this model did not appear to physiologically recapitulate trophoblast development and was not reliable. The primitive endoderm is an epithelium which arises from the inner cell mass during mammalian pre-implantation development. It faces the blastocoel cavity and later gives rise to the extraembryonic parietal and visceral endoderm. When mES cells are grown in suspension they form aggregates of differentiating cells known as embryoid bodies. The outermost cell layer of an embryoid body is an epithelial cell type comparable to the primitive endoderm. Embryoid bodies were used here to study the polarisation and cell fate specification of the primitive endoderm. The outer cells of these embryoid bodies were found to gradually acquire the hallmarks of polarised epithelial cells and express markers of primitive endoderm cell fate. The acquisition of epithelial polarity occurred prior to the maximal expression of cell fate markers. Fgfr/Erk signalling is known to be required for specification of the primitive endoderm, but its role in polarisation of this tissue is less well understood. To investigate the function of this pathway in the primitive endoderm, embryoid bodies were cultured in the presence of a small molecule inhibitor of Mek. This inhibitor caused a loss of expression of markers of primitive endoderm cell fate and maintenance of the pluripotency marker Nanog. In addition, a mislocalisation of apico-basolateral markers and disruption of the epithelial barrier which normally blocks free diffusion across the epithelial cell layer occurred. Two inhibitors of the Fgf receptor elicited similar phenotypes, suggesting that Fgf receptor signalling promotes Erkmediated polarisation. This data shows that the formation of a polarised primitive endoderm layer in embryoid bodies requires the Fgfr/Erk signalling pathway.
Subjects/Keywords: 611.0187; stem cells; Development; preimplantation embryo; epithelial polarity; epithelial
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APA (6th Edition):
Doughton, G. L. (2014). Cell fate specification and polarisation in mouse preimplantation epithelia. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/cell-fate-specification-and-polarisation-in-mouse-preimplantation-epithelia(d8fbae5b-285b-4413-b370-3aa32ad4d607).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619153
Chicago Manual of Style (16th Edition):
Doughton, Gail Louise. “Cell fate specification and polarisation in mouse preimplantation epithelia.” 2014. Doctoral Dissertation, University of Bath. Accessed March 04, 2021.
https://researchportal.bath.ac.uk/en/studentthesis/cell-fate-specification-and-polarisation-in-mouse-preimplantation-epithelia(d8fbae5b-285b-4413-b370-3aa32ad4d607).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619153.
MLA Handbook (7th Edition):
Doughton, Gail Louise. “Cell fate specification and polarisation in mouse preimplantation epithelia.” 2014. Web. 04 Mar 2021.
Vancouver:
Doughton GL. Cell fate specification and polarisation in mouse preimplantation epithelia. [Internet] [Doctoral dissertation]. University of Bath; 2014. [cited 2021 Mar 04].
Available from: https://researchportal.bath.ac.uk/en/studentthesis/cell-fate-specification-and-polarisation-in-mouse-preimplantation-epithelia(d8fbae5b-285b-4413-b370-3aa32ad4d607).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619153.
Council of Science Editors:
Doughton GL. Cell fate specification and polarisation in mouse preimplantation epithelia. [Doctoral Dissertation]. University of Bath; 2014. Available from: https://researchportal.bath.ac.uk/en/studentthesis/cell-fate-specification-and-polarisation-in-mouse-preimplantation-epithelia(d8fbae5b-285b-4413-b370-3aa32ad4d607).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619153
Georgia Tech
9.
Enemchukwu, Nduka Obichukwu.
Bioartificial matrices to modulate epithelial morphogenesis.
Degree: PhD, Mechanical Engineering, 2013, Georgia Tech
URL: http://hdl.handle.net/1853/52938
► Acute injury of major epithelial organ systems (kidney, liver, lung, etc.) is collectively a principal cause of death worldwide. Regenerative medicine promises to meet these…
(more)
▼ Acute injury of major
epithelial organ systems (kidney, liver, lung, etc.) is collectively a principal cause of death worldwide. Regenerative medicine promises to meet these human health challenges by harnessing intrinsic cellular processes to repair or replace damaged tissues.
Epithelial morphogenesis is a hard-wired, multicellular differentiation program that dynamically integrates microenvironmental cues to coordinate cell fate processes including adhesion, migration, proliferation, and polarization. Thus,
epithelial morphogenesis is an instructive mode of tissue assembly, maintenance, and repair. Three-dimensional
epithelial cell cultures in natural basement membrane (BM) extracts produce hollow, spherical cyst structures and have indicated that the BM provides the critical cell adhesion ligands to facilitate cell survival, stimulate proliferation, and promote polarization and lumen formation. However, the utility of natural BMs for detailed studies is generally limited by lot-to-lot variations, uncontrolled cell adhesive interactions, or growth factor contamination.
The goal of this thesis was to engineer bioartificial extracellular matrices (ECM) that would support and modulate
epithelial cyst morphogenesis. We have engineered hydrogels, based on a multi-arm maleimide-terminated poly (ethylene glycol) (PEG-4MAL), that present cell adhesive molecules and enzymatic degradation substrates and promote polarized
epithelial cyst differentiation in vitro.
To investigate the influence of matrix physical and biochemical signals on cyst morphogenesis, we independently varied the polymer weight percentage (wt%), the density of a cell adhesion ligand (RGD), and crosslink degradation rates of the hydrogels. Then, we evaluated functional outcomes including Madin-Darby canine kidney (MDCK II)
epithelial cell survival, proliferation, cyst polarization, and lumen formation. We found that cell proliferation, but not cell survival, was sensitive to the polymer wt%, which is related to elastic modulus and crosslink density. This result defined a working range of PEG-4MAL concentration (3.5% - 4.5%) that promotes robust proliferation. Analysis of mature cysts indicated that 4.0% and 4.5% gels produced cysts resembling those typically grown in type I collagen gels while 3.5% gels produced cysts with higher incidence of inverted polarity and multiple lumens. Perturbation of matrix degradability using a slow-degrading crosslink peptide or matrix metalloproteinase inhibitors showed that the rate of matrix degradation exerts major influence on cyst growth in PEG-4MAL gels. We employed 4.0% PEG-4MAL hydrogels with RGD ligand density ranging over 0 – 2000 uM to discover that (1) lumen formation was eliminated in the absence of RGD, (2) extent of lumen formation increased with increasing RGD concentration, and (3) cyst polarity was inverted below a threshold of integrin binding to RGD.
Together, these results show that the biochemical and physical properties of the matrix, particularly integrin binding and matrix degradability,…
Advisors/Committee Members: García, Andrés (advisor), Nusrat, Asma (committee member), Barker, Thomas H. (committee member), Collard, David M. (committee member), Dixon, J. Brandon (committee member).
Subjects/Keywords: Biomaterials; Polyethylene glycol; Epithelial morphogenesis; Cyst; Extracellular matrix; Epithelial cells
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APA (6th Edition):
Enemchukwu, N. O. (2013). Bioartificial matrices to modulate epithelial morphogenesis. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/52938
Chicago Manual of Style (16th Edition):
Enemchukwu, Nduka Obichukwu. “Bioartificial matrices to modulate epithelial morphogenesis.” 2013. Doctoral Dissertation, Georgia Tech. Accessed March 04, 2021.
http://hdl.handle.net/1853/52938.
MLA Handbook (7th Edition):
Enemchukwu, Nduka Obichukwu. “Bioartificial matrices to modulate epithelial morphogenesis.” 2013. Web. 04 Mar 2021.
Vancouver:
Enemchukwu NO. Bioartificial matrices to modulate epithelial morphogenesis. [Internet] [Doctoral dissertation]. Georgia Tech; 2013. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1853/52938.
Council of Science Editors:
Enemchukwu NO. Bioartificial matrices to modulate epithelial morphogenesis. [Doctoral Dissertation]. Georgia Tech; 2013. Available from: http://hdl.handle.net/1853/52938
Universiteit Utrecht
10.
Cook, E.C.L.
The Expression of RALDH Enzymes by Small Intestinal Epithelial Cells.
Degree: 2012, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/233670
► Retinal dehydrogenase (RALDH) is the key enzyme that regulates the production of retinoic acid (RA), an important mediator of immune responses in the gut. RA…
(more)
▼ Retinal dehydrogenase (RALDH) is the key enzyme that regulates the production of retinoic acid (RA), an important mediator of immune responses in the gut. RA is the biologically active metabolite of vitamin A. Vitamin A is at the same time processed and metabolized in small intestine. RA produced by intestinal
epithelial cells is known to be essential for the induction of characteristic mucosal immune responses by innate immune
cells in the intestinal environment. The expression of RALDH enzymes is tissue-specific, with certain tissues expressing higher concentrations than others, and with variations between the different isoforms of the enzyme. RALDH levels and RA production by the innate immune
cells in the gut have been shown to be regulated by dietary vitamin A. However the expression of RALDH enzymes in the intestinal epithelium does not decrease with different levels of vitamin A in the diet. In the case of RALDH3 it is even up-regulated in mice fed vitamin A deficient diets. It is believed that this is due to a compensatory mechanism. In this review we look at the genetic and environmental factors that can affect the expression of this enzyme in small intestinal
epithelial cells. From this perspective we point at the possible indirect effects that these factors could have on the innate immune
cells in the gut through its effects on the production on RA by intestinal
epithelial cells.
Advisors/Committee Members: Pieters, R.H.H., Mebius, R..
Subjects/Keywords: RALDH; intestinal epithelial cells; vitamin A; retinol
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APA (6th Edition):
Cook, E. C. L. (2012). The Expression of RALDH Enzymes by Small Intestinal Epithelial Cells. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/233670
Chicago Manual of Style (16th Edition):
Cook, E C L. “The Expression of RALDH Enzymes by Small Intestinal Epithelial Cells.” 2012. Masters Thesis, Universiteit Utrecht. Accessed March 04, 2021.
http://dspace.library.uu.nl:8080/handle/1874/233670.
MLA Handbook (7th Edition):
Cook, E C L. “The Expression of RALDH Enzymes by Small Intestinal Epithelial Cells.” 2012. Web. 04 Mar 2021.
Vancouver:
Cook ECL. The Expression of RALDH Enzymes by Small Intestinal Epithelial Cells. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Mar 04].
Available from: http://dspace.library.uu.nl:8080/handle/1874/233670.
Council of Science Editors:
Cook ECL. The Expression of RALDH Enzymes by Small Intestinal Epithelial Cells. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/233670
University of California – Merced
11.
Sater, Ali Abbas Abdul.
Chlamydia induced caspase-1 activation is inflammasome dependent: role and sources of reactive oxygen species in chlamydial growth.
Degree: Quantitative and Systems Biology, 2010, University of California – Merced
URL: http://www.escholarship.org/uc/item/6jr544w6
► Inflammasomes have been extensively characterized in monocytes and macrophages, but not in epithelial cells, which are the preferred host cells for many pathogens. Here we…
(more)
▼ Inflammasomes have been extensively characterized in monocytes and macrophages, but not in
epithelial cells, which are the preferred host cells for many pathogens. Here we show that
cervical epithelial cells express a functional inflammasome. Infection of the cells by Chlamydia
trachomatis leads to activation of caspase-1, through a process requiring the NOD-like receptor
family member NLRP3 and the inflammasome adaptor protein ASC. Secretion of newly
synthesized virulence proteins from the chlamydial vacuole through a type III secretion
apparatus results in efflux of K+ through glibenclamide-sensitive K+ channels, which in turn
stimulates production of reactive oxygen species. Elevated levels of reactive oxygen species
(ROS) are responsible for NLRP3-dependent caspase-1 activation in the infected cells. In
monocytes and macrophages, caspase-1 is involved in processing and secretion of proinflammatory
cytokines such as interleukin-1β. We demonstrate that C. trachomatis or C.
muridarum infection of a monocytic cell line lead to caspase-1 dependent interleukin-1β (IL-1β)
secretion in a NLRP3 inflammasome dependent manner that requires Syk signaling. However, in epithelial cells, which are not known to secrete large quantities of IL-1β, caspase-1 has been
shown previously to enhance lipid metabolism. Here we show that, in cervical epithelial cells,
caspase-1 activation is required for optimal growth of the intracellular chlamydiae. Given the
importance of ROS-induced caspase-1 activation in growth of chlamydial inclusion, we further
investigate the sources of ROS production in epithelial cells following infection with C.
trachomatis. In this study, we provide evidence that NLRX1-dependent mitochondrial generation
of ROS is one of the two sources of ROS production induced by chlamydial infection; the other
source being NADPH oxidase.
Subjects/Keywords: Biology.; Chlamydia.; Epithelial cells.; Caspase-1.
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APA (6th Edition):
Sater, A. A. A. (2010). Chlamydia induced caspase-1 activation is inflammasome dependent: role and sources of reactive oxygen species in chlamydial growth. (Thesis). University of California – Merced. Retrieved from http://www.escholarship.org/uc/item/6jr544w6
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sater, Ali Abbas Abdul. “Chlamydia induced caspase-1 activation is inflammasome dependent: role and sources of reactive oxygen species in chlamydial growth.” 2010. Thesis, University of California – Merced. Accessed March 04, 2021.
http://www.escholarship.org/uc/item/6jr544w6.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sater, Ali Abbas Abdul. “Chlamydia induced caspase-1 activation is inflammasome dependent: role and sources of reactive oxygen species in chlamydial growth.” 2010. Web. 04 Mar 2021.
Vancouver:
Sater AAA. Chlamydia induced caspase-1 activation is inflammasome dependent: role and sources of reactive oxygen species in chlamydial growth. [Internet] [Thesis]. University of California – Merced; 2010. [cited 2021 Mar 04].
Available from: http://www.escholarship.org/uc/item/6jr544w6.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sater AAA. Chlamydia induced caspase-1 activation is inflammasome dependent: role and sources of reactive oxygen species in chlamydial growth. [Thesis]. University of California – Merced; 2010. Available from: http://www.escholarship.org/uc/item/6jr544w6
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Hong Kong
12.
Auyeung, Chun-yee, Natalie.
Fucosyltransferase-5 on
human spermatozoa mediates attachment of spermatozoa to oviductal
epithelial cells.
Degree: 2008, University of Hong Kong
URL: http://hdl.handle.net/10722/52011
Subjects/Keywords: Fucosyltransferases.;
Epithelial cells.
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APA (6th Edition):
Auyeung, Chun-yee, N. (2008). Fucosyltransferase-5 on
human spermatozoa mediates attachment of spermatozoa to oviductal
epithelial cells. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/52011
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Auyeung, Chun-yee, Natalie. “Fucosyltransferase-5 on
human spermatozoa mediates attachment of spermatozoa to oviductal
epithelial cells.” 2008. Thesis, University of Hong Kong. Accessed March 04, 2021.
http://hdl.handle.net/10722/52011.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Auyeung, Chun-yee, Natalie. “Fucosyltransferase-5 on
human spermatozoa mediates attachment of spermatozoa to oviductal
epithelial cells.” 2008. Web. 04 Mar 2021.
Vancouver:
Auyeung, Chun-yee N. Fucosyltransferase-5 on
human spermatozoa mediates attachment of spermatozoa to oviductal
epithelial cells. [Internet] [Thesis]. University of Hong Kong; 2008. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10722/52011.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Auyeung, Chun-yee N. Fucosyltransferase-5 on
human spermatozoa mediates attachment of spermatozoa to oviductal
epithelial cells. [Thesis]. University of Hong Kong; 2008. Available from: http://hdl.handle.net/10722/52011
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Cornell University
13.
Ju, Chia-Hsin.
Innate Immunity Of The Intestinal Epithelium During Toxoplasma Gondii Infection.
Degree: PhD, Veterinary Medicine, 2011, Cornell University
URL: http://hdl.handle.net/1813/33498
► The single layer of epithelial cells that line the intestinal tract provides both a physiologic and immunologic barrier for microbes and foreign antigens. Intestinal epithelial…
(more)
▼ The single layer of
epithelial cells that line the intestinal tract provides both a physiologic and immunologic barrier for microbes and foreign antigens. Intestinal
epithelial cells are differentiated into different functional subtypes and have regulatory roles in maintaining the homeostasis of the gut as well as initiating inflammatory responses during pathogenic infections. Toxoplasma gondii is a widespread zoonotic protozoan parasite that infects warm-blooded animals. Infection usually occurs through ingestion of T. gondii contaminated food or water. Therefore, natural route of infection has revealed the importance of
epithelial cell response in influencing the outcome of the local and systemic immune response. In this thesis, I examined the innate immune response of intestinal
epithelial cell during T. gondii infection. The immediate response upon intestinal
epithelial and parasite contact revealed that T. gondii infected
epithelial cells elicited MAPK phosphorylation, NF-!B activation, and secretion of IL-8 as well as several other inflammatory cytokines and chemokines in
epithelial cells. I found that activation of MAPK and production of IL-8 was dependent on the MyD88 signaling pathway, and TLR2 was sufficient for parasite induced signaling in HEK 293 transfected
cells. In addition, TLR2 signaling plays an important role in modulating of the local intestinal environment by inducing migration of dendritic
cells into the follicle-associated epithelium of Peyer's patches. Using the susceptible C57BL/6 mice as a model, morphological changes of different intestinal
epithelial cell subtypes in response to peroral T. gondii infection were further characterized. Oral infection with T. gondii ME49 cysts induced intestinal pathology and morphological changes of different
epithelial cell subtypes. Mice deficient in TLR2 were more resistant to intestinal pathology and associated morphological changes. Parasite-derived TLR2 stimulatory molecules were found to be present in the supernatant collected from T. gondii infected
cells. Therefore, potential biological significance of the activity in supernatants from T. gondii infected
cells was also investigated. I found that the supernatants from T. gondii infected
cells enhanced luminal antigen uptake and induced TLR2-dependent migration of dendritic
cells to the follicle associated epithelium of Peyer's patches. Furthermore, analysis of the supernatant revealed several T. gondii proteins, and the GPI-anchored surface antigen-1 contributes to TLR2 stimulatory activity. In summary, this dissertation explores the innate immune response of the intestinal epithelium during T. gondii infection. The signaling pathways of intestinal
epithelial cells elicited by the parasite as well as the modulation of the local intestinal environment can help us better understand the immunopathogenesis of T. gondii infection.
Advisors/Committee Members: Leifer, Cynthia Anne (chair), Denkers, Eric Young (committee member), Marquis, Helene (committee member), Clark, Theodore G. (committee member).
Subjects/Keywords: Innate immunity; Intestinal epithelial cells; Toxoplasma gondii
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APA (6th Edition):
Ju, C. (2011). Innate Immunity Of The Intestinal Epithelium During Toxoplasma Gondii Infection. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/33498
Chicago Manual of Style (16th Edition):
Ju, Chia-Hsin. “Innate Immunity Of The Intestinal Epithelium During Toxoplasma Gondii Infection.” 2011. Doctoral Dissertation, Cornell University. Accessed March 04, 2021.
http://hdl.handle.net/1813/33498.
MLA Handbook (7th Edition):
Ju, Chia-Hsin. “Innate Immunity Of The Intestinal Epithelium During Toxoplasma Gondii Infection.” 2011. Web. 04 Mar 2021.
Vancouver:
Ju C. Innate Immunity Of The Intestinal Epithelium During Toxoplasma Gondii Infection. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1813/33498.
Council of Science Editors:
Ju C. Innate Immunity Of The Intestinal Epithelium During Toxoplasma Gondii Infection. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/33498
McMaster University
14.
Dizzell, Sara.
THE ROLE OF FEMALE SEX HORMONES AND LACTOBACILLI ON GENITAL EPITHELIAL CELL BARRIER FUNCTIONS AND INNATE IMMUNE RESPONSES IN THE PRESENCE AND ABSENCE OF HIV.
Degree: MSc, 2017, McMaster University
URL: http://hdl.handle.net/11375/21229
► Background: Approximately 40% of global human immunodeficiency virus-1 (HIV) transmission occurs in the female genital tract (FGT). Epithelial cells lining the FGT comprise the first…
(more)
▼ Background: Approximately 40% of global human immunodeficiency virus-1 (HIV) transmission occurs in the female genital tract (FGT).
Epithelial cells lining the FGT comprise the first barrier to HIV-1 entry. The functions of these
cells are influenced by female sex hormones and the mucosal microbiota. Studies have suggested that hormonal environment and a dysbiosis of the FGT microbiota may lead to inflammation in the genital mucosa and enhance HIV acquisition. A Lactobacillus dominant microenvironment in the FGT is considered to have protective functions against sexually transmitted pathogens, however the interaction between sex hormones and lactobacilli and their effect on
epithelial cell functions remains to be determined.
Methods of Study: For these studies, primary genital
epithelial cells (GECs) were isolated from hysterectomy tissues obtained following patient consent. GEC cultures were grown to confluence on cell culture inserts in the presence or absence of the female sex hormones estrogen (E2), progesterone (P4), or medroxyprogesterone acetate (MPA). Polarized monolayers were exposed to two probiotic strains of Lactobacillus: L. reuteri (RC-14) or L. rhamnosus (GR-1), or the most common strain of bacteria found in the FGT, L. crispatus in the presence or absence of HIV-1. Cell viability, barrier integrity, and innate inflammatory factors were among the primary measures performed.
Results: In our system, cell viability was unaltered in the presence of Lactobacillus species and/or female sex hormones. All three strains of bacteria (L. crispatus and probiotic lactobacilli GR-1 and RC-14) significantly increased GEC barrier integrity, as measured by transepithelial electrical resistance (TER). Both GR-1 and RC-14 significantly reduced GEC barrier permeability as measured by a dextran dye leakage assay, whereas L. crispatus did not. Conversely, hormones did not alter barrier integrity nor barrier permeability. However, hormones did alter secretion of cytokines and chemokins by GECs. GECs grown in the presence of estrogen decreased TNF-α, IL-1α, IL-1β and IL-8 secretion in comparison to no hormone treatment, while GECs grown in the presence of MPA significantly decreased MIP-1α and TNF-α secretion. In the presence of HIV both GR-1 and RC-14 were able to confer an increase in barrier integrity similar to that observed with GR-1 and RC-14 treatment alone. Addionally, GECs grown in the presence of E2 and MPA displayed a less inflammatory (TNF-α, IL-1α, and IL-1β) environment when exposed to HIV compared to no hormone and P4. Interstingly, the decrease in inflammation was not observed when measuring chemokines such as IL-8 and RANTES. Furthermore, probiotic bacteria were able to significantly reduce HIV mediated increases in TNF-α when grown in the presence of no hormone, P4, and MPA. A similar trend was observed for GECs grown in the presence of E2 however, given that E2 reduced the TNF-α response mediated by HIV, results were not significant. Overall, probiotic lactobacilii GR-1 and RC-14 enhanced GEC barrier…
Advisors/Committee Members: Kaushic, Charu, Medical Sciences (Molecular Virology and Immunology Program).
Subjects/Keywords: Epithelial Cells; Hormones; HIV; Microbiota; Lactobacilli
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APA (6th Edition):
Dizzell, S. (2017). THE ROLE OF FEMALE SEX HORMONES AND LACTOBACILLI ON GENITAL EPITHELIAL CELL BARRIER FUNCTIONS AND INNATE IMMUNE RESPONSES IN THE PRESENCE AND ABSENCE OF HIV. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/21229
Chicago Manual of Style (16th Edition):
Dizzell, Sara. “THE ROLE OF FEMALE SEX HORMONES AND LACTOBACILLI ON GENITAL EPITHELIAL CELL BARRIER FUNCTIONS AND INNATE IMMUNE RESPONSES IN THE PRESENCE AND ABSENCE OF HIV.” 2017. Masters Thesis, McMaster University. Accessed March 04, 2021.
http://hdl.handle.net/11375/21229.
MLA Handbook (7th Edition):
Dizzell, Sara. “THE ROLE OF FEMALE SEX HORMONES AND LACTOBACILLI ON GENITAL EPITHELIAL CELL BARRIER FUNCTIONS AND INNATE IMMUNE RESPONSES IN THE PRESENCE AND ABSENCE OF HIV.” 2017. Web. 04 Mar 2021.
Vancouver:
Dizzell S. THE ROLE OF FEMALE SEX HORMONES AND LACTOBACILLI ON GENITAL EPITHELIAL CELL BARRIER FUNCTIONS AND INNATE IMMUNE RESPONSES IN THE PRESENCE AND ABSENCE OF HIV. [Internet] [Masters thesis]. McMaster University; 2017. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/11375/21229.
Council of Science Editors:
Dizzell S. THE ROLE OF FEMALE SEX HORMONES AND LACTOBACILLI ON GENITAL EPITHELIAL CELL BARRIER FUNCTIONS AND INNATE IMMUNE RESPONSES IN THE PRESENCE AND ABSENCE OF HIV. [Masters Thesis]. McMaster University; 2017. Available from: http://hdl.handle.net/11375/21229
Texas A&M University
15.
Botchlett, Rachel Erin.
Nutrient Regulation of PFKFB3/iPFK2 and Its Role in Regulating Diet-Induced Inflammation in Intestinal Epithelial Cells.
Degree: PhD, Nutrition, 2015, Texas A&M University
URL: http://hdl.handle.net/1969.1/156209
► The gene PFKFB3 encodes for inducible 6-phosphofructo-2-kinase (iPFK2), an important regulatory enzyme of glycolysis. It is shown that PFKFB3/iPFK2 links metabolic and inflammatory pathways in…
(more)
▼ The gene PFKFB3 encodes for inducible 6-phosphofructo-2-kinase (iPFK2), an important regulatory enzyme of glycolysis. It is shown that PFKFB3/iPFK2 links metabolic and inflammatory pathways in adipose tissue; however, whether it functions in the same manner within small intestine, where nutrients are assimilated and first interact with the body, is unknown. Therefore, the present study firstly investigated how diet, macronutrients, e.g. glucose and palmitate, and bacterial metabolites influence PFKFB3/iPFK2 expression, and secondly determined how altered gene expression relates to inflammatory responses in small intestinal
epithelial cells (IECs).
HFD feeding and in vitro palmitate treatment were associated with reduced PFKFB3/iPFK2 but increased proinflammatory responses. LFD feeding and glucose treatment showed the opposite result. In vitro overexpression of PFKFB3/iPFK2 lead to reduced proinflammatory responses while inhibition of PFKFB3/iPFK2 was associated with increased inflammatory markers. Treatment with the bacterial metabolite indole stimulated PFKFB3/iPFK2 and reduced the generation of inflammation.
Together these findings indicate that macronutrients differentially regulate PFKFB3/iPFK2 expression in IECs, where carbohydrates stimulate PFKFB3/iPFK2 and saturated fats contribute to proinflammatory mechanisms. Further, results confirm an anti-inflammatory ability of PFKFB3/iPFK2 within IECs and suggest an additional anti-inflammatory mechanism of action of indole in regulating inflammation through PFKFB3/iPFK2.
Advisors/Committee Members: Wu, Chaodong (advisor), Awika, Joseph (committee member), Riechman, Steven (committee member), Walzem, Rosemary (committee member).
Subjects/Keywords: Overnutrition-induced inflammation; PFKFB3; intestinal epithelial cells
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APA (6th Edition):
Botchlett, R. E. (2015). Nutrient Regulation of PFKFB3/iPFK2 and Its Role in Regulating Diet-Induced Inflammation in Intestinal Epithelial Cells. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156209
Chicago Manual of Style (16th Edition):
Botchlett, Rachel Erin. “Nutrient Regulation of PFKFB3/iPFK2 and Its Role in Regulating Diet-Induced Inflammation in Intestinal Epithelial Cells.” 2015. Doctoral Dissertation, Texas A&M University. Accessed March 04, 2021.
http://hdl.handle.net/1969.1/156209.
MLA Handbook (7th Edition):
Botchlett, Rachel Erin. “Nutrient Regulation of PFKFB3/iPFK2 and Its Role in Regulating Diet-Induced Inflammation in Intestinal Epithelial Cells.” 2015. Web. 04 Mar 2021.
Vancouver:
Botchlett RE. Nutrient Regulation of PFKFB3/iPFK2 and Its Role in Regulating Diet-Induced Inflammation in Intestinal Epithelial Cells. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1969.1/156209.
Council of Science Editors:
Botchlett RE. Nutrient Regulation of PFKFB3/iPFK2 and Its Role in Regulating Diet-Induced Inflammation in Intestinal Epithelial Cells. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156209
University of Newcastle
16.
Hsu, Alan (Chen-Yu).
Mechanism of infection of bronchial epithelial cells by human and avian influenza viruses.
Degree: PhD, 2010, University of Newcastle
URL: http://hdl.handle.net/1959.13/917312
► Research Doctorate - Doctor of Philosophy (PhD)
Respiratory epithelial cells including bronchial epithelial cells (BECs) are the initial site of infection with influenza viruses, and…
(more)
▼ Research Doctorate - Doctor of Philosophy (PhD)
Respiratory epithelial cells including bronchial epithelial cells (BECs) are the initial site of infection with influenza viruses, and the anti-viral responses elicited by these cells are a critical first line of defense against respiratory viral infection and can induce effective adaptive immune responses. However, relatively little is known about the importance of this innate anti-viral response, and the magnitude and effectiveness of this response is poorly understood. In addition to human influenza, avian influenza viruses are a potential source of future pandemics, therefore it is also critical to examine the effectiveness of the host anti-viral system to low and high pathogenic avian influenza viruses. Anti-viral responses to a human influenza H3N2, a low pathogenic avian influenza H11N9 and a high pathogenic avian influenza H5N1 was profiled using Calu-3 cells and primary bronchial epithelial cells (pBECs) to model proximal airway cells and A549 cells to reflect alveolar cells. The susceptibility of BECs to influenza infection was not solely dependent on the sialic acid-bearing glycoprotein expression and was affected by the apoptosis and anti-viral responses after infection. The earlier and greater anti-viral signaling and protein production correlated with the control of infection. However the H3N2 strain resulted in a delay in anti-viral signaling and impaired release of type I and type III interferons (IFNs) compared to the H11N9 virus. The differences in anti-viral induction between H3N2 and H11N9 were partly due to the influenza non-structural (NS) 1 protein. The gene encoding for NS1 was transfected into the BECs and the H3N2 NS1 induced a greater inhibition of anti-viral responses compared to the H11N9 NS1. Regardless of this inhibition by the influenza viruses, the constitutive secretion of IFN-β by BECs played a more critical role in inducing late anti-viral signaling via type I IFN receptors and was crucial in limiting viral infection by apoptosis. Infection with H5N1 resulted in a complete abolishment of this response in the infected BECs, including those induced by the constitutive IFN-β. This was due to the robust inhibition of host anti-viral responses by the NS1 protein of H5N1. This study characterizes anti-influenza virus responses in airway epithelial cells and shows for the first time that constitutive IFN-β release plays an important role in initiating protective late IFN-stimulated responses during influenza infection in airway epithelial cells. In addition, the subversion of human anti-viral responses may be an important requirement for influenza viruses to adapt to the human host and induce disease. Furthermore by understanding both of these avenues will identify targets for potential therapeutics.
Advisors/Committee Members: University of Newcastle. Faculty of Health, School of Medicine and Public Health.
Subjects/Keywords: influenza; innate immunity; bronchial epithelial cells
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APA (6th Edition):
Hsu, A. (. (2010). Mechanism of infection of bronchial epithelial cells by human and avian influenza viruses. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/917312
Chicago Manual of Style (16th Edition):
Hsu, Alan (Chen-Yu). “Mechanism of infection of bronchial epithelial cells by human and avian influenza viruses.” 2010. Doctoral Dissertation, University of Newcastle. Accessed March 04, 2021.
http://hdl.handle.net/1959.13/917312.
MLA Handbook (7th Edition):
Hsu, Alan (Chen-Yu). “Mechanism of infection of bronchial epithelial cells by human and avian influenza viruses.” 2010. Web. 04 Mar 2021.
Vancouver:
Hsu A(. Mechanism of infection of bronchial epithelial cells by human and avian influenza viruses. [Internet] [Doctoral dissertation]. University of Newcastle; 2010. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1959.13/917312.
Council of Science Editors:
Hsu A(. Mechanism of infection of bronchial epithelial cells by human and avian influenza viruses. [Doctoral Dissertation]. University of Newcastle; 2010. Available from: http://hdl.handle.net/1959.13/917312
University of Texas Southwestern Medical Center
17.
Ly, Peter 1986-.
Characterization and Selective Targeting of Aneuploid Human Colonic Epithelial Cells.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/ETD-UTSWMED-2012-12-57
► Aneuploidy, an abnormal number of chromosomes, occurs in the vast majority of sporadic colorectal cancer patients. Despite this observation, the cellular advantages conferred by recurring…
(more)
▼ Aneuploidy, an abnormal number of chromosomes, occurs in the vast majority of sporadic colorectal cancer patients. Despite this observation, the cellular advantages conferred by recurring cytogenetic alterations are poorly understood and targeted therapies selective to aneuploid
cells are currently non-existent. Here, we provide evidence that serum-free passage of originally diploid, immortalized human colonic
epithelial cells give rise to the acquisition of trisomy 7 (1CT+7), an aneuploidy detected in over 40% of colorectal adenomas. Pre-existing 1CT+7
cells within the original population were undetectable through GTG-banding and fluorescent in situ hybridization analysis, suggesting a conversion of diploid
cells to an aneuploid state. Compared to their isogenic diploid counterpart, 1CT+7
cells express higher levels of the epidermal growth factor receptor (EGFR, located on chromosome 7p). Treatment with the pharmacological adenosine analog 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR) completely halted proliferation of 1CT+7
cells and reduced both metabolic consumption and production in vitro. Unexpectedly, treatment of 1CT+7
cells with AICAR led to a reversible 3.5-fold reduction in EGFR overexpression. AICAR-induced depletion of EGFR protein can be abrogated through inhibition of the proteasome with MG132. AICAR also heavily promoted EGFR ubiquitination in cell-based immunoprecipitation assays, suggesting enhanced degradation of EGFR protein mediated by the proteasome. Moreover, treatment with AICAR reduced EGFR protein levels in a panel of human colorectal cancer cell lines in vitro and in xenograft tumors in vivo. Our data collectively supports the compound AICAR as a novel inhibitor of EGFR protein abundance and as a potential anticancer agent for aneuploidy-driven colorectal cancer. In summary, we have isolated and characterized isogenic human colonic
epithelial cells that represent recurrent chromosomal acquisitions in sporadic colorectal cancer and demonstrate how it may be possible to selectively target these
cells for therapeutic intervention.
Advisors/Committee Members: Lum, Lawrence, Pearson, Gray W., Pandita, Tej K..
Subjects/Keywords: Aminoimidazole Carboxamide; Colorectal Neoplasms; Epithelial Cells
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APA (6th Edition):
Ly, P. 1. (2012). Characterization and Selective Targeting of Aneuploid Human Colonic Epithelial Cells. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/ETD-UTSWMED-2012-12-57
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ly, Peter 1986-. “Characterization and Selective Targeting of Aneuploid Human Colonic Epithelial Cells.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 04, 2021.
http://hdl.handle.net/2152.5/ETD-UTSWMED-2012-12-57.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ly, Peter 1986-. “Characterization and Selective Targeting of Aneuploid Human Colonic Epithelial Cells.” 2012. Web. 04 Mar 2021.
Vancouver:
Ly P1. Characterization and Selective Targeting of Aneuploid Human Colonic Epithelial Cells. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2012-12-57.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ly P1. Characterization and Selective Targeting of Aneuploid Human Colonic Epithelial Cells. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2012-12-57
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
반, 가영.
Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target.
Degree: 2016, Ajou University
URL: http://repository.ajou.ac.kr/handle/201003/13080
;
http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021457
► Background: Autophagy and genetic predisposition have been suggested to potentially play roles in the development of asthma. However, little is known about the role of…
(more)
▼ Background: Autophagy and genetic predisposition have been suggested to potentially play roles in the development of asthma. However, little is known about the role of autophagy in the pathogenesis of severe asthma.
Objective: We compared autophagy in the sputum granulocytes, peripheral blood cells (PBCs), and peripheral blood eosinophils (PBEs) between patients with severe asthma and those with non-severe asthma and investigated the functional effects of autophagy.
Methods: We enrolled 36 patients with severe asthma, 14 with non-severe asthma, and 23 normal healthy controls in this study. Sputum granulocytes, PBCs, and PBEs were isolated from each subject. Autophagy was evaluated based on the expression of microtubule-associated protein light chain 3 (LC3) by western blot, confocal microscopy, transmission electron microscopy, and flow cytometry. IL-8 levels were measured by ELISA. To induce autophagy, HL-60 cells, SAECs, and A549 cells were treated with IL-5, IL-1β, and TNF-α. To inhibit autophagy, PI3K inhibitors (LY29400 and 3-methyladenine [3-MA]) and hydroxychloroquine (HCQ) were used. Knockdown of ATG5 and Beclin-1 was performed in A549 cells, and the therapeutic effects of dexamethasone were evaluated.
Results: Higher autophagy levels were noted in sputum granulocytes, PBCs, and PBEs from patients with severe asthma than from patients with non-severe asthma and healthy controls (P<0.05 for all). IL-5 increased autophagy levels in both PBCs and PBEs (P<0.05). 3-MA attenuated the increased expression of LC3-II and eosinophil cationic protein in HL-60 cells induced by IL-5 (P=0.034 for both). Dexamethasone did not affect autophagy levels in PBEs. IL-1β increased LC3-II expression and IL-8 production (P<0.01) in SAECs, and this was attenuated by LY294002, 3-MA, HCQ, and knockdown of ATG5 and Beclin-1 (in A549 cells) (P<0.01).
Conclusions and clinical relevance: Autophagy could play a role in the pathogenesis of severe asthma. Autophagy modulation may be a novel therapeutic target for conventional therapy-resistant severe asthma.
Ⅰ. INTRODUCTION 1
II. MATERIALS AND METHODS 4
A. MATERIALS 4
1. Study subjects 4
2. Antibodies and reagents 4
B. METHODS 5
1. Cell culture 5
2. Sputum induction and granulocyte isolation 5
3. Human PBC isolation and autophagy induction 6
III. RESULTS 12
IV. DISCUSSION 24
V. CONCLUSION 30
REFERENCES 31
국문요약 36
Doctor
Advisors/Committee Members: 대학원 의학과, 201325251, 반, 가영.
Subjects/Keywords: Autophagy; eosinophils; severe asthma; treatment; epithelial cells
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APA (6th Edition):
반, . (2016). Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/13080 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021457
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
반, 가영. “Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target.” 2016. Thesis, Ajou University. Accessed March 04, 2021.
http://repository.ajou.ac.kr/handle/201003/13080 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021457.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
반, 가영. “Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target.” 2016. Web. 04 Mar 2021.
Vancouver:
반 . Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target. [Internet] [Thesis]. Ajou University; 2016. [cited 2021 Mar 04].
Available from: http://repository.ajou.ac.kr/handle/201003/13080 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021457.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
반 . Autophagy mechanisms in sputum and peripheral blood cells of patients with severe asthma: a new therapeutic target. [Thesis]. Ajou University; 2016. Available from: http://repository.ajou.ac.kr/handle/201003/13080 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021457
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Houston
19.
-8465-3791.
Defining Anti-Tumorigenic and Anti-Inflammatory Effects Mediated By Estrogen Receptor Beta In Colon Epithelial Cells.
Degree: PhD, Biology and Biochemistry, 2015, University of Houston
URL: http://hdl.handle.net/10657/2018
► Despite its slow development and our capacity for early detection using endoscopy, colorectal cancer remains the second leading cause of cancer death in the United…
(more)
▼ Despite its slow development and our capacity for early detection using endoscopy, colorectal cancer remains the second leading cause of cancer death in the United States. Epidemiological studies indicate a role for estrogen in protecting against colorectal cancer. Estrogen receptor β (ERβ) has been found to be the main ER in the colon. Cell line studies have implicated that ERβ has anti-tumorigenic and anti-proliferative effects on colon cancer
cells lines while human epidemiological data suggest that polymorphisms in the ERβ gene are associated with greater cancer risk and lower survival.
Overexpression of ERβ changes the miRnome. Here we show that ERβ can alleviate the progression of colon cancer by inhibiting proliferation through decreasing MYC transcription and therefore decreasing levels of miR-17-92 (OncomiR-1). This is reflected in a decrease in cell number, decrease in migration, and increase in apoptosis. Furthermore, addition of miR-17 using miRNA mimics reverses the effects of ERβ. This demonstrates that ERβ influences not only the transcriptome, but also the miRnome and that the miRnome can be potential targets for novel treatment approaches.
ERβ upregulates miR-205 and downregulates PROX1. This study uncovers the pathway in which ERβ downregulates the transcription factor PROX1 by upregulating miR-205 directly. MiR-205 then targets PROX1 mRNA for degradation. This results in the
cells adopting a more adhesive phenotype and reduces the metastatic potential. This study uncovers the potential for ERβ to be anti-metastatic in addition to being anti-proliferative and anti-inflammatory.
ERβ modulates the NFkB inflammatory cascade. One of ERβ’s attributes is that it has anti-inflammatory capabilities. Using a whole genome approach, this study reveals that ERβ can modulate the NFkB inflammatory network. In SW480
cells, ERβ downregulates NFkB transcription factors and induces apoptosis. In HT29
cells, ERβ prevents p65 subunit of NFkB from translocating to the nucleus. Overall, ERβ attenuates the NFkB signaling cascade by attenuating genes related to inflammation while enhancing genes related to apoptosis and cellular defenses. This makes ERβ a useful target for anti-inflammatory drug treatments for patients suffering from chronic inflammatory diseases.
Advisors/Committee Members: Williams, Cecilia M. (advisor), Lin, Chin-Yo (committee member), Gunaratne, Preethi H. (committee member), Lee, Mong-Hong (committee member).
Subjects/Keywords: Estrogen receptor beta; Colon cancer epithelial cells
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APA ·
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APA (6th Edition):
-8465-3791. (2015). Defining Anti-Tumorigenic and Anti-Inflammatory Effects Mediated By Estrogen Receptor Beta In Colon Epithelial Cells. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/2018
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Chicago Manual of Style (16th Edition):
-8465-3791. “Defining Anti-Tumorigenic and Anti-Inflammatory Effects Mediated By Estrogen Receptor Beta In Colon Epithelial Cells.” 2015. Doctoral Dissertation, University of Houston. Accessed March 04, 2021.
http://hdl.handle.net/10657/2018.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
MLA Handbook (7th Edition):
-8465-3791. “Defining Anti-Tumorigenic and Anti-Inflammatory Effects Mediated By Estrogen Receptor Beta In Colon Epithelial Cells.” 2015. Web. 04 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
-8465-3791. Defining Anti-Tumorigenic and Anti-Inflammatory Effects Mediated By Estrogen Receptor Beta In Colon Epithelial Cells. [Internet] [Doctoral dissertation]. University of Houston; 2015. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10657/2018.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Council of Science Editors:
-8465-3791. Defining Anti-Tumorigenic and Anti-Inflammatory Effects Mediated By Estrogen Receptor Beta In Colon Epithelial Cells. [Doctoral Dissertation]. University of Houston; 2015. Available from: http://hdl.handle.net/10657/2018
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
20.
Moreno Layseca, Paulina.
The role of β1-integrin in normal and oncogene-mediated proliferation in breast epithelia.
Degree: PhD, 2015, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-1integrin-in-normal-and-oncogenemediated-proliferation-in-breast-epithelia(635388a7-5382-4cb5-82d1-d098b2092e2e).html
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634970
► Luminal epithelial cells in the mammary gland require two types of signals to proliferate: soluble signals (growth factor signals) and signals from the extracellular matrix…
(more)
▼ Luminal epithelial cells in the mammary gland require two types of signals to proliferate: soluble signals (growth factor signals) and signals from the extracellular matrix (ECM). The composition of the ECM is sensed by adhesion receptors such as integrins. Integrins modulate cell behaviour and play a key role in cell cycle entry. Altered integrin expression and signalling has been associated with breast cancer and studies using mouse mammary epithelial cells (MECs) have shown that the absence of β1-integrin induces growth arrest. However, it is not completely understood how integrins transduce the signals from the plasma membrane to the nucleus to induce cell cycle entry. Thus, the first aim of this project was to determine how β1-integrin controls proliferation in MECs. I established a model to study the effects of depleting β1-integrin using the FSK7 mammary epithelial cell line. The proliferation defect observed in this β1-integrin knockdown model was rescued by expressing a constitutively active Rac1 or Pak. Moreover, inhibiting Rac1 or Pak prevented normal proliferation in MECs in a similar fashion as β1-integrin depletion. Furthermore, in this thesis I have identified the complex comprised of Src, paxillin and p130Cas as a potential link between β1-integrin and Rac1. These results provide an insight into the mechanism that regulates proliferation downstream of β1-integrin. During breast cancer initiation, β1-integrin signals are disrupted. This indicates that additional signals must be driving proliferation during tumorigenesis. Therefore, the second aim of this project was to test whether expression of breast oncogenes can overcome the proliferation defect present in β1-integrin null cells. In order to do so, an oncogenic ErbB2, a constitutively active form of Akt (myrAkt) and the Notch1 intracellular domain (NICD) were transfected in the β1-integrin knockdown MECs. The results showed that ErbB2 overcomes the need for β1-integrin by signalling to Pak. NICD does not require β1-integrin to drive proliferation by an unknown mechanism. Expression of myrAkt did not restore normal levels of proliferation in β1-integrin depleted MECs. This finding suggests that Akt is not sufficient to induce cell cycle entry by itself and instead, both Akt and Erk signalling are needed to exert this function. This work has further delineated the specific signals controlling proliferation downstream of β1-integrin, and has provided a model to test the dependence of oncogenes for β1-integrin to drive proliferation in MECs. These studies are important to understand the role of β1-integrin in breast cancer formation and to define the types of breast cancer where β1-integrin can be used as an effective therapeutic target.
Subjects/Keywords: 611; Integrins; Proliferation; Mammary epithelial cells
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APA (6th Edition):
Moreno Layseca, P. (2015). The role of β1-integrin in normal and oncogene-mediated proliferation in breast epithelia. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-1integrin-in-normal-and-oncogenemediated-proliferation-in-breast-epithelia(635388a7-5382-4cb5-82d1-d098b2092e2e).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634970
Chicago Manual of Style (16th Edition):
Moreno Layseca, Paulina. “The role of β1-integrin in normal and oncogene-mediated proliferation in breast epithelia.” 2015. Doctoral Dissertation, University of Manchester. Accessed March 04, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-1integrin-in-normal-and-oncogenemediated-proliferation-in-breast-epithelia(635388a7-5382-4cb5-82d1-d098b2092e2e).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634970.
MLA Handbook (7th Edition):
Moreno Layseca, Paulina. “The role of β1-integrin in normal and oncogene-mediated proliferation in breast epithelia.” 2015. Web. 04 Mar 2021.
Vancouver:
Moreno Layseca P. The role of β1-integrin in normal and oncogene-mediated proliferation in breast epithelia. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Mar 04].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-1integrin-in-normal-and-oncogenemediated-proliferation-in-breast-epithelia(635388a7-5382-4cb5-82d1-d098b2092e2e).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634970.
Council of Science Editors:
Moreno Layseca P. The role of β1-integrin in normal and oncogene-mediated proliferation in breast epithelia. [Doctoral Dissertation]. University of Manchester; 2015. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-1integrin-in-normal-and-oncogenemediated-proliferation-in-breast-epithelia(635388a7-5382-4cb5-82d1-d098b2092e2e).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634970
Hong Kong University of Science and Technology
21.
Li, Youjun.
Epithelial cell growth and polarity establishment.
Degree: 2015, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-87439
;
https://doi.org/10.14711/thesis-b1449009
;
http://repository.ust.hk/ir/bitstream/1783.1-87439/1/th_redirect.html
► The apical transmembrane protein Crumbs (Crb) is evolutionarily conserved in metazoan, and acts as a master cell polarity and growth regulator in polarized epithelia. Crb…
(more)
▼ The apical transmembrane protein Crumbs (Crb) is evolutionarily conserved in metazoan, and acts as a master cell polarity and growth regulator in polarized epithelia. Crb intra-cellular functions are mediated by its highly conserved 37-residue cytoplasmic tail (Crb-CT). However, the mechanistic basis governing Crb's role in cell polarity and growth remains unclear. Here, I discover that the PDZ-SH3-GK tandem of PALS1 directly binds to Crb-CT with a dissociation constant of 70 nM, which is -100-fold stronger than the PALSI PDZ/Crb-CT interaction. The crystal structure of the PALS1 PDZ-SH3-GK/Crb-CT complex reveals that the PDZ-SH3-GK forms a structural supramodule with all three domains contributing to the tight binding to Crb. Mutations disrupting the tertiary interactions of the PDZ-SH3-GK supramodule weaken the PALSl/Crb interaction and compromise PALSI-mediated polarity establishment in MDCK cysts. These findings not only elucidate the molecular basis regard to Crb/PALSl complex in determining the apical-basal cell polarity, but also indicate a common PDZ-SH3-GK mode for other members of MAGUKs in specific target recognition. I also biochemically and structurally characterized the direct interaction between Crb-CT and Moesin FERM domain, which provides a direct linkage from the plasma membrane to the actin cytoskeleton. The 1.5 Å resolution crystal structure of the Moesin-FERM/Crb-CT complex reveals a typical FERM/FBM binding mode, in which the FBM of Crb-CT forms a short β-sheet and fits into the canonical F3-binding site. To our surprise, the PBM of Crb-CT also contributes to the binding to Moesin-FERM, by occupying the InsP3 binding site at the Fl/F3 cleft, implying that Crb-CT may mimic the role of Ptdlns(4,5)P2 in the activation of the ERM family proteins. Interestingly, phosphorylation of Crb-CT by aPKC disrupts the Crb/Moesin association, but has no impact on the Crb/PALSl interaction. The above findings suggest that establishment of cell polarity promotes aPKC-mediated Crb phosphorylation and subsequent Crb/Moesin complex dissociation. Thus, Crb becomes to be stably associated with tight junction-localized PALS1 in polarized epithelia. It is likely that aPKC-mediated phosphorylation of Crb functions to switch epithelial cells from proliferation to contact-mediated growth inhibition. Actin cytoskeleton is a major regulator of the Hippo signaling pathway, and angiomotin (AMOT) can relay the signals initiated by F-actin structural changes to control YAP activity. I show in the fourth part of this dissertation and by high resolution crystal structures that the Lats1/2 binding site on the Merlin FERM domain is physically blocked by Merlin's auto-inhibitory tail. AMOT binding releases the auto-inhibition and promotes Merlin's binding to Lats1/2. Phosphorylation of Ser518 outside the Merlin's auto-inhibitory tail prevents AMOT from binding, and thus inhibits Hippo kinase activation. These findings reveal that AMOT and Merlin interface the cortical actin filaments and the…
Subjects/Keywords: Epithelial cells
; Growth
; Cytoplasm
; Membrane proteins
; Structure
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APA ·
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Export
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APA (6th Edition):
Li, Y. (2015). Epithelial cell growth and polarity establishment. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-87439 ; https://doi.org/10.14711/thesis-b1449009 ; http://repository.ust.hk/ir/bitstream/1783.1-87439/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Youjun. “Epithelial cell growth and polarity establishment.” 2015. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021.
http://repository.ust.hk/ir/Record/1783.1-87439 ; https://doi.org/10.14711/thesis-b1449009 ; http://repository.ust.hk/ir/bitstream/1783.1-87439/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Youjun. “Epithelial cell growth and polarity establishment.” 2015. Web. 04 Mar 2021.
Vancouver:
Li Y. Epithelial cell growth and polarity establishment. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2015. [cited 2021 Mar 04].
Available from: http://repository.ust.hk/ir/Record/1783.1-87439 ; https://doi.org/10.14711/thesis-b1449009 ; http://repository.ust.hk/ir/bitstream/1783.1-87439/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li Y. Epithelial cell growth and polarity establishment. [Thesis]. Hong Kong University of Science and Technology; 2015. Available from: http://repository.ust.hk/ir/Record/1783.1-87439 ; https://doi.org/10.14711/thesis-b1449009 ; http://repository.ust.hk/ir/bitstream/1783.1-87439/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Hong Kong University of Science and Technology
22.
Xie, Changyan LIFS.
Mechanosensitive gating of CFTR in epithelial cell volume regulation.
Degree: 2014, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-88929
;
https://doi.org/10.14711/thesis-b1448978
;
http://repository.ust.hk/ir/bitstream/1783.1-88929/1/th_redirect.html
► Regulatory volume decrease (RVD) is crucial for cells to survive swelling. The volume sensitive outwardly rectifying anion channel (VSOR) is considered to be the major…
(more)
▼ Regulatory volume decrease (RVD) is crucial for cells to survive swelling. The volume sensitive outwardly rectifying anion channel (VSOR) is considered to be the major anion channel governing RVD in many tissues and cells. The cystic fibrosis transmembrane conductance regulator (CFTR) functions as both an intracellular ligand-gated channel and a regulator of other channels and transporters in many epithelial tissues. The loss of CFTR impaired RVD of epithelial cells with elusive mechanism. Our lab found that stretch activates CFTR and thus we hypothesize that CFTR may mediate epithelial RVD by directly responding to the mechanical stress induced by cell-swelling. Here we examined the molecular identity of volume-sensing anion channel in epithelia RVD by blocking VSOR and CFTR activities. In Calu-3 cells, an airway epithelial cell-line with highly expressed CFTR, hypotonicity-induced short circuit current (Isc) is sensitive to CFTR specific inhibitor but not to VSOR inhibitors. The lsc was independent of cytoplasmic ca2+ levels and cAMP levels, suggesting the mechanosensitivity of CFTR played a role. Moreover, genetic suppression or ablation of CFTR markedly reduced swelling induced lsc. In cultured CHO cells and freshly isolated intestinal crypts expressing CFTR, blocking CFTR abolished RVD triggered by hypotonicity. RVD is important in cell migration. We found that exogenous CFTR expression accelerated cell migration in HEK293T cells. Surprisingly, GSS1D, a CFTR mutation lacking cAMP-induced chloride channel activity, functioned like wild-type CFTR in swelling- or stretch-induced lsc, RVD and cell migration. Furthermore, cell swelling also activated CFTR in cell-attached membrane patches in Calu-3 cells. Thus, our studies showed that both wild-type and G551D CFTR might be involved in epithelial RVD by responding to the mechanical stress induced by cell-swelling. Our findings also might explain the relative milder phenotype of cystic fibrosis (CF) patients carrying G551D mutation and shed light on developing novel mechanical treatment for CF.
Subjects/Keywords: Cystic fibrosis
; Epithelial cells
; Cellular control mechanisms
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APA (6th Edition):
Xie, C. L. (2014). Mechanosensitive gating of CFTR in epithelial cell volume regulation. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-88929 ; https://doi.org/10.14711/thesis-b1448978 ; http://repository.ust.hk/ir/bitstream/1783.1-88929/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Xie, Changyan LIFS. “Mechanosensitive gating of CFTR in epithelial cell volume regulation.” 2014. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021.
http://repository.ust.hk/ir/Record/1783.1-88929 ; https://doi.org/10.14711/thesis-b1448978 ; http://repository.ust.hk/ir/bitstream/1783.1-88929/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Xie, Changyan LIFS. “Mechanosensitive gating of CFTR in epithelial cell volume regulation.” 2014. Web. 04 Mar 2021.
Vancouver:
Xie CL. Mechanosensitive gating of CFTR in epithelial cell volume regulation. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2014. [cited 2021 Mar 04].
Available from: http://repository.ust.hk/ir/Record/1783.1-88929 ; https://doi.org/10.14711/thesis-b1448978 ; http://repository.ust.hk/ir/bitstream/1783.1-88929/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Xie CL. Mechanosensitive gating of CFTR in epithelial cell volume regulation. [Thesis]. Hong Kong University of Science and Technology; 2014. Available from: http://repository.ust.hk/ir/Record/1783.1-88929 ; https://doi.org/10.14711/thesis-b1448978 ; http://repository.ust.hk/ir/bitstream/1783.1-88929/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Hong Kong University of Science and Technology
23.
Chen, Xianwei LIFS.
Investigation of tumor growth in epithelium.
Degree: 2016, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-97893
;
https://doi.org/10.14711/thesis-b1610644
;
http://repository.ust.hk/ir/bitstream/1783.1-97893/1/th_redirect.html
► The neoplastic Tumor Suppressor Genes (nTSGs) are a group of genes initially identified with genetic screenings in Drosophila (fruit fly). Mutations of nTSGs lead to…
(more)
▼ The neoplastic Tumor Suppressor Genes (nTSGs) are a group of genes initially identified with genetic screenings in Drosophila (fruit fly). Mutations of nTSGs lead to loss of epithelial polarity and transform the mutant cells into highly proliferative and invasive tissue. Importantly, the mammalian homologs of Drosophila nTSGs show highly conserved functions in epithelial organization and proliferation control. In a genetic mosaic experiment, we noticed that nTSG mutant cells underwent over proliferation in folded epithelial regions while they were eliminated through cell competition in a flat epithelial sheet. The epithelial architecture-dependent variation of tumor development has also been reported in human carcinoma cases. These evidence points to our hypothesis that epithelial curvature is an important factor determining the phenotypic outcomes of nTSG mutations. To test this hypothesis, we developed methods to manipulate the mechanical properties of epithelial cells and observed that increase of cellular tension led to ectopic fold formation and promoted nTSG mutant cell growth in the flat epithelium. We also show the activation of JAK/STAT pathway is necessary for the survival and growth of the mutant cells. In addition, we show that diverse curvature of epithelial cell sheets leads to different actomyosin activity and Yki activity, indicating that epithelial curvature may influence nTSG mutant cell growth outcomes through modulating Yki activity. Our study provides a new insight into the carcinogenesis where biomechanical signals can play a significant role. Keywords: nTSGs Fly tumor Curvature JAK/STAT pathway Yki
Subjects/Keywords: Tumors
; Genetic aspects
; Epithelial cells
; Diseases
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chen, X. L. (2016). Investigation of tumor growth in epithelium. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-97893 ; https://doi.org/10.14711/thesis-b1610644 ; http://repository.ust.hk/ir/bitstream/1783.1-97893/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Xianwei LIFS. “Investigation of tumor growth in epithelium.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021.
http://repository.ust.hk/ir/Record/1783.1-97893 ; https://doi.org/10.14711/thesis-b1610644 ; http://repository.ust.hk/ir/bitstream/1783.1-97893/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Xianwei LIFS. “Investigation of tumor growth in epithelium.” 2016. Web. 04 Mar 2021.
Vancouver:
Chen XL. Investigation of tumor growth in epithelium. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 04].
Available from: http://repository.ust.hk/ir/Record/1783.1-97893 ; https://doi.org/10.14711/thesis-b1610644 ; http://repository.ust.hk/ir/bitstream/1783.1-97893/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen XL. Investigation of tumor growth in epithelium. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-97893 ; https://doi.org/10.14711/thesis-b1610644 ; http://repository.ust.hk/ir/bitstream/1783.1-97893/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Hong Kong University of Science and Technology
24.
Shen, Yusheng SENG.
Topographic modulation of epithelial morphogenesis and mechanics, and membrane protein distribution.
Degree: 2017, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-100428
;
https://doi.org/10.14711/thesis-991012550869503412
;
http://repository.ust.hk/ir/bitstream/1783.1-100428/1/th_redirect.html
► The extracellular matrix (ECM) exhibits rich tissue-specific topography and composition and plays a crucial role in initiating the biochemical and biomechanical signaling required for organizing…
(more)
▼ The extracellular matrix (ECM) exhibits rich tissue-specific topography and composition and plays a crucial role in initiating the biochemical and biomechanical signaling required for organizing cells into distinct tissues during development. Dissecting these biochemical and biomechanical cues provided by the ECM is instrumental in the understanding of many biological processes. Over the past 2 decades, micropatterning and nanopatterning toolkits that are used to engineer cell-substrate interfaces have emerged, rapidly expanded and widely used among bioengineers and biologists for the study of cell biology. Among these studies, surface topography has been widely recognized to participate in controlling cellular functions including cell adhesion, migration, proliferation, polarization and differentiation. However, very few studies have evaluated how surface topography affects epithelial tissue-like morphogenesis and how surface topography modulates the distribution of membrane proteins that are normally thought not associated with the adhesion complex. Meanwhile, studying cell behavior in a defined microenvironment by using surface microengineering tools offers tremendous advantage over traditional uncontrolled methods. This direction is also inadequately explored especially in the study of cell mechanics in a controlled microenvironment. This work offers a peek at the potential role of surface topography in the aforementioned aspects. Specifically, the effect of substrate nanotopography on the tissue-like morphogenesis of several types of epithelial cells was examined. We demonstrated that substrate nanotopography, one of the first physical cues detected by cells, can by itself induce epithelial cyst formation. We further explored the possibility of generating probe-accessible, size-controllable epithelial cysts by using micropatterning and combined it with atomic force microscopy to characterize the cyst mechanics. By using this platform, we estimated the elasticity of the cyst monolayer and showed that the presence of a luminal space influences cyst mechanics substantially, which could be attributed to polarization and tissue-level coordination. More interestingly, the results from force-relaxation experiments showed that the cysts also displayed tissue-level poroelastic and power-law characteristics. Last but not least, we evaluated the potential role of a membrane protein, ANO1 (anoctamin 1), in membrane curvature sensing by subjecting exogenously overexpressed ANO1 to various membrane curvatures that are externally induced by substrate topography such as micropillar and microgroove patterns. Markedly, membrane distribution of ANO1 was profoundly modulated by substrate topography as characterized by the clustering at positively curved membrane regions. Analysis of the clustering dynamics revealed that ANO1 potentially associates with adhesion complexes to form large-scale assemblies.
Subjects/Keywords: Epithelial cells
; Morphogenesis
; Membrane proteins
; Surfaces (Technology)
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shen, Y. S. (2017). Topographic modulation of epithelial morphogenesis and mechanics, and membrane protein distribution. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-100428 ; https://doi.org/10.14711/thesis-991012550869503412 ; http://repository.ust.hk/ir/bitstream/1783.1-100428/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shen, Yusheng SENG. “Topographic modulation of epithelial morphogenesis and mechanics, and membrane protein distribution.” 2017. Thesis, Hong Kong University of Science and Technology. Accessed March 04, 2021.
http://repository.ust.hk/ir/Record/1783.1-100428 ; https://doi.org/10.14711/thesis-991012550869503412 ; http://repository.ust.hk/ir/bitstream/1783.1-100428/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shen, Yusheng SENG. “Topographic modulation of epithelial morphogenesis and mechanics, and membrane protein distribution.” 2017. Web. 04 Mar 2021.
Vancouver:
Shen YS. Topographic modulation of epithelial morphogenesis and mechanics, and membrane protein distribution. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2017. [cited 2021 Mar 04].
Available from: http://repository.ust.hk/ir/Record/1783.1-100428 ; https://doi.org/10.14711/thesis-991012550869503412 ; http://repository.ust.hk/ir/bitstream/1783.1-100428/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shen YS. Topographic modulation of epithelial morphogenesis and mechanics, and membrane protein distribution. [Thesis]. Hong Kong University of Science and Technology; 2017. Available from: http://repository.ust.hk/ir/Record/1783.1-100428 ; https://doi.org/10.14711/thesis-991012550869503412 ; http://repository.ust.hk/ir/bitstream/1783.1-100428/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Utah State University
25.
Shah, Jigna D.
Stress Response And Pathogenesis of <i>Salmonella enterica</i> serovar Typhimurium.
Degree: PhD, Nutrition, Dietetics, and Food Sciences, 2011, Utah State University
URL: https://digitalcommons.usu.edu/etd/900
► Salmonella is a food-borne pathogen that leads to substantial illness worldwide. The clinical syndromes associated with Salmonella infection are enteric (typhoid) fever and gastroenteritis,…
(more)
▼ Salmonella is a food-borne pathogen that leads to substantial illness worldwide. The clinical syndromes associated with Salmonella infection are enteric (typhoid) fever and gastroenteritis, in healthy humans. Typhoid fever is caused by host-adapted S. Typhi and S. Paratyphi. Gastroenteritis is caused by serovars usually referred to as non typhoidal Salmonellae (NTS). In recent years, an increasing number of outbreaks due to NTS, despite increased efforts in food safety, were reported because of persistence of Salmonella in the food chain. Thus I hypothesized that Salmonella is able to withstand stresses in the environment and treatments used during food processing for its elimination and thereby able to develop resistance against subsequent stress encounters. The effect of cold, peroxide, and acid was tested on survival of S. Typhimurium and the survival was persistent under cold stress (5°C) for up to 240 h. Pre-adaptation to cold stress (5°C, 5 h) also increased survival of S. Typhimurium during subsequent exposure to acid stress (pH 4.0, 90 min) by repressing hydroxyl radical formation. Cold stress (5°C, 48 h) to S. Typhimurium significantly (p < 0.05) increased its adhesion and invasion in intestinal iv
epithelial cells. This phenotype was attributed to a pair of protein-protein interactorsacting as receptors on microbial (STM2699) and host cell surface (SPTAN1). Cold stress significantly (q < 0.05) induced STM2699 in S. Typhimurium and SPTAN1 was significantly (q < 0.05) induced in pithelial
cells upon infection with cold-stressed S. Typhimurium. Cold stress to S. Typhimurium also significantly (q < 0.05) induced genes related to virulence such as type 3 secretion system apparatus and effectors genes, prophage genes, and plasmid genes and they remain induced upon infection of
epithelial cells with additional induction of spv genes on the plasmid. Infection of
epithelial cells with cold-stressed S. Typhimurium significantly (p < 0.05) increased activation of caspase 9 and 3/7. Cold-stressed S. Typhimurium switched metabolism from aerobic respiration to fermentation and it persisted during infection of
epithelial cells. As a result, short chain fatty acids formate and acetate, which act as diffusible signal for invasion, were detected in significantly (q < 0.05) high amounts in extracellular media of
cells infected with cold-stressed S. Typhimurium supporting the phenotype of high adhesion and invasion of cold-stressed S. Typhimurium in
epithelial cells.
Advisors/Committee Members: Bart C. Weimer, Marie Walsh, Dong Chen, ;.
Subjects/Keywords: Cold stress; Epithelial cells; Salmonella; Microbiology
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APA (6th Edition):
Shah, J. D. (2011). Stress Response And Pathogenesis of <i>Salmonella enterica</i> serovar Typhimurium. (Doctoral Dissertation). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/900
Chicago Manual of Style (16th Edition):
Shah, Jigna D. “Stress Response And Pathogenesis of <i>Salmonella enterica</i> serovar Typhimurium.” 2011. Doctoral Dissertation, Utah State University. Accessed March 04, 2021.
https://digitalcommons.usu.edu/etd/900.
MLA Handbook (7th Edition):
Shah, Jigna D. “Stress Response And Pathogenesis of <i>Salmonella enterica</i> serovar Typhimurium.” 2011. Web. 04 Mar 2021.
Vancouver:
Shah JD. Stress Response And Pathogenesis of <i>Salmonella enterica</i> serovar Typhimurium. [Internet] [Doctoral dissertation]. Utah State University; 2011. [cited 2021 Mar 04].
Available from: https://digitalcommons.usu.edu/etd/900.
Council of Science Editors:
Shah JD. Stress Response And Pathogenesis of <i>Salmonella enterica</i> serovar Typhimurium. [Doctoral Dissertation]. Utah State University; 2011. Available from: https://digitalcommons.usu.edu/etd/900
University of Hong Kong
26.
Zhou, Yirun.
The anti-oxidant potential
of different flavonoids in human bronchial epithelial
cells.
Degree: 2015, University of Hong Kong
URL: http://hdl.handle.net/10722/221520
► Oxidative stress is known to play a pathogenetic role in neurodegenerative, cardiovascular, lung and liver diseases. It increases as a result of an imbalance between…
(more)
▼ Oxidative stress is known to play a
pathogenetic role in neurodegenerative, cardiovascular, lung and
liver diseases. It increases as a result of an imbalance between
the production of oxidants and the activity of the antioxidant
defense system. The antioxidants and free radical scavengers
eliminate the excessive reactive oxygen species, prevent their
formation and promote the activity of endogenous anti-oxidant
mechanism. Flavonoids are well-known to possess anti-oxidant
properties. In the present study, the anti-oxidant potentials of
different flavonoids from different sub-families were examined in
airway epithelial cells. Moreover, experiments were designed to
examine whether or not flavonoids reduce the oxidative stress in
the airway epithelium due to bacterial infection, and if so,
whether or not they produce the effects by activating the two major
endogenous anti-oxidant systems, catalase and glutathione.
Human
bronchial epithelial BEAS-2B cells were treated with different
flavonoids in the absence and presence of the bacterial endotoxin,
lipopolysaccharides (LPS). The concentration of 8-isoprostane, a
biomarker for oxidative stress, in the culture medium was measured,
as an indication of the antioxidant potentials of the flavonoids.
The activity of catalase and the cellular level of glutathione in
BEAS-2B cells after the treatments were also evaluated. Among the
17 flavonoids tested, pelargonidin was the only flavonoid that
could reduce 8-isoprostane levels in conditions with and without
LPS. Quercetin showed an anti-oxidant effect only in cells without
LPS treatment while phloretin reduced 8-isoprostane level only in
cells treated with LPS. Daidzein was found to have a pro-oxidant
effect in the presence of LPS. Unexpectedly, quercetin reduced
catalase activity in BEAS-2B cells not treated with LPS, and had no
effect on the cellular glutathione level. Daidzein did not affect
catalase activity and glutathione level, similarly to
catechin[which served as the “negative control” for flavonoids in
the study(as it did not affect the 8-isoprostane level in
conditions with and without LPS)].
In conclusion, not all
flavonoids produce anti-oxidant effects in human bronchial
epithelial cells; only quercetin, pelargonidin and phloretin appear
to offer anti-oxidative protection. However, quercetin inhibits
catalase in BEAS-2B cells without LPS treatment, thus suggesting a
possible pro-oxidant action under basal conditions. Therefore,
cautions should be taken in considering the use of quercetin as a
health supplement against cellular oxidative stress. Similarly, the
observation that daidzein may have pro-oxidant effects in
conditions with LPS also poses a warning for the use of this
flavonoid as a health supplement. The proposal that flavonoids have
pro-oxidant effects appears contradictive with their reported
effects in some other studies; the discrepancy may be explained by
the differences in the cell types, the biomarker and the
concentration of flavonoid used. Therefore, the present findings
prompt the need for a…
Subjects/Keywords: Epithelial cells;
Flavonoids
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhou, Y. (2015). The anti-oxidant potential
of different flavonoids in human bronchial epithelial
cells. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/221520
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zhou, Yirun. “The anti-oxidant potential
of different flavonoids in human bronchial epithelial
cells.” 2015. Thesis, University of Hong Kong. Accessed March 04, 2021.
http://hdl.handle.net/10722/221520.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zhou, Yirun. “The anti-oxidant potential
of different flavonoids in human bronchial epithelial
cells.” 2015. Web. 04 Mar 2021.
Vancouver:
Zhou Y. The anti-oxidant potential
of different flavonoids in human bronchial epithelial
cells. [Internet] [Thesis]. University of Hong Kong; 2015. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10722/221520.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zhou Y. The anti-oxidant potential
of different flavonoids in human bronchial epithelial
cells. [Thesis]. University of Hong Kong; 2015. Available from: http://hdl.handle.net/10722/221520
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Hong Kong
27.
溫錫剛.
Establishment and
characterization of human ovarian surface epithelialcells
immortalized by human papilloma viral oncogenes.
Degree: 1997, University of Hong Kong
URL: http://hdl.handle.net/10722/34849
Subjects/Keywords: Oncogenes.;
Epithelial cells.
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
溫錫剛. (1997). Establishment and
characterization of human ovarian surface epithelialcells
immortalized by human papilloma viral oncogenes. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/34849
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
溫錫剛. “Establishment and
characterization of human ovarian surface epithelialcells
immortalized by human papilloma viral oncogenes.” 1997. Thesis, University of Hong Kong. Accessed March 04, 2021.
http://hdl.handle.net/10722/34849.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
溫錫剛. “Establishment and
characterization of human ovarian surface epithelialcells
immortalized by human papilloma viral oncogenes.” 1997. Web. 04 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
溫錫剛. Establishment and
characterization of human ovarian surface epithelialcells
immortalized by human papilloma viral oncogenes. [Internet] [Thesis]. University of Hong Kong; 1997. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10722/34849.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
溫錫剛. Establishment and
characterization of human ovarian surface epithelialcells
immortalized by human papilloma viral oncogenes. [Thesis]. University of Hong Kong; 1997. Available from: http://hdl.handle.net/10722/34849
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Massey University
28.
Biet, Juliane.
Effect of mechanical stress o the integrity, signalling mechanisms and function of bovine mammary epithelial cells : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Manawatū, Palmerston North, New Zealand
.
Degree: 2014, Massey University
URL: http://hdl.handle.net/10179/7214
► Mammary gland engorgement due to milk accumulation in late lactation leads to changes in cell morphology and has been recognised as a potential key initiator…
(more)
▼ Mammary gland engorgement due to milk accumulation in late lactation leads to
changes in cell morphology and has been recognised as a potential key initiator
of involution and remodelling of the mammary gland. The physical distension of
mammary epithelial cells (MEC), due to udder filling, is likely to result in
mechanical tension on cell-cell and cell-matrix interactions. Cell-cell and cellmatrix
junctions provide tissue integrity, promote cell polarity, guarantee sufficient
communication between cells to ensure synchronised milk secretion and support
cell survival. Their disruption may be one of the early initiators of the mammary
gland remodelling process. As a consequence, the primary goal of this study was
to determine the potential effects of MEC stretch on changes in cell sensing
within the mechanical micro-environment in the initiation of bovine MEC
involution. During this investigation, particular emphasis was put on three
potential mechanosensors: tight junctions (TJ), focal adhesions (FA) and primary
cilia (PC), and their regulation in the early stages of involution using in vivo and in
vitro experimental approaches. Static, biaxial in vitro cell stretch and acute
physical distension in vivo resulted in changes in TJ protein expression levels
implying a potential disruption of cell-cell communication as well as
communication with the cell‟s cytoskeleton. Furthermore, down-regulation of Akt
and pAkt following different periods of mechanical strain applied in vitro and
decreased levels of pAkt following acute physical distension in vivo indicated a
disruption of β1-integrin-FAK survival signalling through the PI3K-Akt pathway
downstream of FA interactions. Increased numbers of ciliated MEC following
extended periods of non-milking indicated a dedifferentiation of MEC.
Furthermore, increased levels of STAT6 transcription (part of PC signalling
following mechanical stimulation) factor indicates the initiation of macrophage
accumulation and promotion of tissue remodelling of the bovine mammary gland.
In conclusion, this study supports the hypothesis that local factors play an
important role during bovine mammary gland involution and that mechanical
stimulation may play a part in the initiation of this process.
Subjects/Keywords: Mammary glands;
Epithelial cells;
Bovine mammary glands
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APA (6th Edition):
Biet, J. (2014). Effect of mechanical stress o the integrity, signalling mechanisms and function of bovine mammary epithelial cells : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Manawatū, Palmerston North, New Zealand
. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/7214
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Biet, Juliane. “Effect of mechanical stress o the integrity, signalling mechanisms and function of bovine mammary epithelial cells : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Manawatū, Palmerston North, New Zealand
.” 2014. Thesis, Massey University. Accessed March 04, 2021.
http://hdl.handle.net/10179/7214.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Biet, Juliane. “Effect of mechanical stress o the integrity, signalling mechanisms and function of bovine mammary epithelial cells : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Manawatū, Palmerston North, New Zealand
.” 2014. Web. 04 Mar 2021.
Vancouver:
Biet J. Effect of mechanical stress o the integrity, signalling mechanisms and function of bovine mammary epithelial cells : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Manawatū, Palmerston North, New Zealand
. [Internet] [Thesis]. Massey University; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10179/7214.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Biet J. Effect of mechanical stress o the integrity, signalling mechanisms and function of bovine mammary epithelial cells : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Manawatū, Palmerston North, New Zealand
. [Thesis]. Massey University; 2014. Available from: http://hdl.handle.net/10179/7214
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
The Ohio State University
29.
Lehman, Teresa Ann.
Studies in human skin epithelial cell carcinogenesis
.
Degree: PhD, Graduate School, 1987, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487332636474889
Subjects/Keywords: Chemistry; Epithelial cells
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Lehman, T. A. (1987). Studies in human skin epithelial cell carcinogenesis
. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487332636474889
Chicago Manual of Style (16th Edition):
Lehman, Teresa Ann. “Studies in human skin epithelial cell carcinogenesis
.” 1987. Doctoral Dissertation, The Ohio State University. Accessed March 04, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1487332636474889.
MLA Handbook (7th Edition):
Lehman, Teresa Ann. “Studies in human skin epithelial cell carcinogenesis
.” 1987. Web. 04 Mar 2021.
Vancouver:
Lehman TA. Studies in human skin epithelial cell carcinogenesis
. [Internet] [Doctoral dissertation]. The Ohio State University; 1987. [cited 2021 Mar 04].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487332636474889.
Council of Science Editors:
Lehman TA. Studies in human skin epithelial cell carcinogenesis
. [Doctoral Dissertation]. The Ohio State University; 1987. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487332636474889
University of Missouri – Columbia
30.
Bruney, Lana.
Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis.
Degree: 2014, University of Missouri – Columbia
URL: http://hdl.handle.net/10355/48202
► Epithelial ovarian cancer (EOC) is one of the most common gynecologic malignancies, generally developing in women over the age of forty. When EOC are diagnosed…
(more)
▼ Epithelial ovarian cancer (EOC) is one of the most common gynecologic malignancies, generally developing in women over the age of forty. When EOC are diagnosed prior to metastatic dissemination, the overall 5-year survival rate is 92%; however, nearly 85% of women with EOC are diagnosed with metastasis already present, dropping the survival rate to less than 30%. EOC, arises, arguably, from the single layer of
cells that cover the ovary or fallopian tube. Metastatic ovarian tumors develop once an
epithelial cell transforms, inducing detachment from the primary tumor site. These shed
cells travel throughout the peritoneal cavity, escaping anoikis to survive as single
cells and multicellular aggregates (MCA), and metastasize intraperitoneally through adhesion to and invasion of the mesothelial cell layer covering the peritoneum, the primary microenvironment for ovarian cancer metastasis. These mesothelial
cells lie atop a collagen type I-rich extracellular matrix; subsequent to the initial attachment of ovarian cancer
cells, proteolytic activity catalyzes migration through the mesothelial monolayer and promotes invasion of the sub-mesothelial matrix. Elucidating the early molecular mechanisms involved in this metastatic process, specifically the adhesion of EOC
cells to mesothelial
cells and penetration of the associated sub-mesothelial extracellular matrix, is essential to the development of future therapeutic agents. Enzymatic activity of matrix type 1 metalloproteinase (MT1-MMP), a transmembrane proteinase that degrades interstitial collagen, has been shown to be critical to this process. MT1-MMP activity has been directly implicated in both the invasion of the sub-mesothelial collagen I matrix, and in the shedding of metastatic MCA, but the molecular mechanisms behind these events are not completely understood. Considering the well-established role of MT1-MMP in the EOC metastatic process, identification of the molecules contributing to these pro-metastatic phenotypes is critical to future understanding of EOC metastatic spread. This research investigated the initial adhesive and invasive events of ovarian cancer metastasis, as associated with MT1-MMP proteolytic activity. Specifically, the effect of MT1-MMP activity on ovarian tumor cell ectodomain shedding and the in vitro, relationship between MT1-MMP and a potential phosphorylator, integrin linked kinase (ILK), on adhesion and invasion was assessed. Investigations utilized in vitro models of homotypic and heterotypic cell-cell adhesion, meso-mimetic invasion assays, and ex vivo tissue explants. Results suggest that ILK activity may catalyze phosphorylation of MT1-MMP to promote pro-metastatic events, including strengthening of adhesive contacts, invasion of the collagen-rich sub-mesothelial matrix, and MCA formation. Additionally, MT1-MMP expression may induce MUC16/CA-125 ectodomain shedding, which may then expose integrins at the ovarian tumor cell surface for high affinity cell-cell and cell-ECM binding.
Advisors/Committee Members: Stack, M. Sharon, 1959- (advisor).
Subjects/Keywords: Epithelial cells; Metalloproteinases; Ovaries – Cancer; Tumors
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Record Details
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bruney, L. (2014). Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/48202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bruney, Lana. “Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis.” 2014. Thesis, University of Missouri – Columbia. Accessed March 04, 2021.
http://hdl.handle.net/10355/48202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bruney, Lana. “Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis.” 2014. Web. 04 Mar 2021.
Vancouver:
Bruney L. Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis. [Internet] [Thesis]. University of Missouri – Columbia; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10355/48202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bruney L. Membrane type 1 matrix metalloproteinase proteolytic activity in initial adhesive and invasive events of ovarian cancer metastasis. [Thesis]. University of Missouri – Columbia; 2014. Available from: http://hdl.handle.net/10355/48202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations
