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University of Ottawa
1.
Li, Aotian.
Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors
.
Degree: 2020, University of Ottawa
URL: http://hdl.handle.net/10393/40473 
► Biocatalytic membranes (BMs) have promising applications in a diversity of fields including food, pharmaceutical and water treatment industries. Of particular relevance, Alcalase is a commercially…
(more)
▼ Biocatalytic membranes (BMs) have promising applications in a diversity of fields including food, pharmaceutical and water treatment industries. Of particular relevance, Alcalase is a commercially important protease that has been applied for the production of peptides from the hydrolysis of proteins. In this study, two different approaches were applied for the modification of electrospun polyacrylonitrile nanofibrous membranes (EPNMs) for Alcalase immobilization. The first approach is alkali modification of EPNMs followed by EDC/NHS coupling for covalent bonding with Alcalase, whereas the other is based on polydopamine coating with or without glutaraldehyde grafting as a covalent linker. Immobilized Alcalase on these prepared BMs were studied and compared with free enzymes. It was found that the stabilities of Alcalase on BMs created using both approaches were improved, which enabled their reuse of 10 cycles with significant retention of enzymatic activity. A continuous reactor housing BMs were tested for hydrolysis of both model substrate, azo-casein and soybean meal protein (SMP). It was found that decreasing flux could improve the extent of hydrolysis and that a single-layer reactor can hydrolyze about 50% of the substrate to peptides with the molecular weight of 10 kDa or less. Hydrolysis of SMPs was demonstrated in a continuous five-layer BM reactor and both BMs showed excellent hydrolysis capacity. This study provides the groundwork for the development of high-efficiency BM for continuous and cost-effective protein hydrolysis for the production of value-added peptides.
Subjects/Keywords: Biocatalytic Membrane;
Enzyme Immobilization;
Electrospun PAN Nanofibrous Membrane;
Protein Hydrolysis;
Continuous Reactor
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APA (6th Edition):
Li, A. (2020). Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/40473
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Aotian. “Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors
.” 2020. Thesis, University of Ottawa. Accessed January 22, 2021.
http://hdl.handle.net/10393/40473.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Aotian. “Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors
.” 2020. Web. 22 Jan 2021.
Vancouver:
Li A. Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors
. [Internet] [Thesis]. University of Ottawa; 2020. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10393/40473.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li A. Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors
. [Thesis]. University of Ottawa; 2020. Available from: http://hdl.handle.net/10393/40473
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Kentucky
2.
Islam, Mohammad Saiful.
MICROFILTRATION MEMBRANE PORE FUNCTIONALIZATION APPROACHES FOR CHLORO-ORGANIC REMEDIATION TO HEAVY METAL SORPTION.
Degree: 2020, University of Kentucky
URL: https://uknowledge.uky.edu/cme_etds/121
► Microfiltration polyvinylidene fluoride (PVDF) membranes have distinct advantage for open structure in terms of high internal surface area and ease of access in the pore…
(more)
▼ Microfiltration polyvinylidene fluoride (PVDF) membranes have distinct advantage for open structure in terms of high internal surface area and ease of access in the pore domain. Functionalization of PVDF membranes with different functional groups (-COOH, -OH, -SH) enables responsive (pH, temperature) properties to membrane, tuning of effective pore size, controlling permeate flux. PVDF microfiltration membrane functionalization with suitable responsive polymer such as poly acrylic acid (PAA) to incorporate carboxyl (-COOH) group enables further modification of functionalized PAA-PVDF membranes for different application ranging from catalysis, bio reactor to heavy metal sorption platform. As a catalytic reactor bed, this PAA-PVDF membranes are very desirable platform for in-situ synthesis of catalytic nanoparticles for conducting a wide range of reactions. As a bio reactor, PAA-PVDF membrane with a net charge have been used to electrostatically immobilize enzymes for conducting catalytic reactions. Functionalization of PVDF membrane also allow for the development of high capacity heavy metal sorbents by modifying existing functional groups (-COOH) to other functional groups (-SH) to adsorb heavy metal cations from contaminated water.
Hydrophilic polymers with carboxylic (-COOH) groups are studied in different functionalization processes especially in preparation of responsive (pH) membranes. To understand the role of membrane pore polymerization condition on the properties of functionalized membrane a systematic study has been conducted, specifically, the effects of polymerization on the membrane mass gain, water permeability, Pd-Fe nanoparticle (NP) loading, of pore functionalized polyvinylidene fluoride (PVDF) membranes. In this study, monomer (acrylic acid (AA)) and cross-linker (N, N′- methylene-bis (acrylamide)) concentrations were varied from 10 to 20 wt% of polymer solution and 0.5-2 mol% of monomer concentration, respectively. Results showed that responsive behavior of membrane could be tuned in terms of water permeability over a range of 270-1 Lm-2 h-1 bar-1, which is a function of water pH. The NP size on the membrane surface was found in the range of 16-23 nm. NP loading was found to vary from 0.21 to 0.94 mg per cm2 of membrane area depending on the variation of available carboxyl groups in membrane pore domain.
The NPs functionalized membranes were then tested as a platform for the degradation of 3,3',4,4',5-pentachlorobiphenyl (PCB 126) and understand the effect of NP loading of the rate of degradation of PCB 126. The observed batch reaction rate (Kobs) for PCB 126 degradation for per mg of catalyst loading was found 0.08-0.1 h-1. Degradation study in convective flow mode shows 98.6% PCB 126 is degraded at a residence time of 46.2 s. The corresponding surface area normalized reaction rate (Ksa) is found about two times higher than Ksa of batch degradation; suggesting elimination of the effect of diffusion resistance for degradation of PCB 126 in convective flow mode operation.
A layer-by-layer approach…
Subjects/Keywords: Microfiltration PVDF Membrane; Nanoparticles; Catalytic Membrane Reactor; Enzyme Immobilized Membrane; Thiol Functionalized Membrane; Hollow Nanoparticles; Membrane Science
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Islam, M. S. (2020). MICROFILTRATION MEMBRANE PORE FUNCTIONALIZATION APPROACHES FOR CHLORO-ORGANIC REMEDIATION TO HEAVY METAL SORPTION. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/cme_etds/121
Chicago Manual of Style (16th Edition):
Islam, Mohammad Saiful. “MICROFILTRATION MEMBRANE PORE FUNCTIONALIZATION APPROACHES FOR CHLORO-ORGANIC REMEDIATION TO HEAVY METAL SORPTION.” 2020. Doctoral Dissertation, University of Kentucky. Accessed January 22, 2021.
https://uknowledge.uky.edu/cme_etds/121.
MLA Handbook (7th Edition):
Islam, Mohammad Saiful. “MICROFILTRATION MEMBRANE PORE FUNCTIONALIZATION APPROACHES FOR CHLORO-ORGANIC REMEDIATION TO HEAVY METAL SORPTION.” 2020. Web. 22 Jan 2021.
Vancouver:
Islam MS. MICROFILTRATION MEMBRANE PORE FUNCTIONALIZATION APPROACHES FOR CHLORO-ORGANIC REMEDIATION TO HEAVY METAL SORPTION. [Internet] [Doctoral dissertation]. University of Kentucky; 2020. [cited 2021 Jan 22].
Available from: https://uknowledge.uky.edu/cme_etds/121.
Council of Science Editors:
Islam MS. MICROFILTRATION MEMBRANE PORE FUNCTIONALIZATION APPROACHES FOR CHLORO-ORGANIC REMEDIATION TO HEAVY METAL SORPTION. [Doctoral Dissertation]. University of Kentucky; 2020. Available from: https://uknowledge.uky.edu/cme_etds/121
University of New South Wales
3.
Ji, Chao.
Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.
Degree: Chemical Engineering, 2017, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/58253
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true
► The increasing worldwide contamination of freshwater system with micro-pollutants emerges as a critical environmental problem, which has driven the search for novel mitigation approaches. The…
(more)
▼ The increasing worldwide contamination of freshwater system with micro-pollutants emerges as a critical environmental problem, which has driven the search for novel mitigation approaches. The use of enzymes such as laccase as biocatalyst has been recognised as a promising approach for micro-pollutants removal. However, rapid denaturation of the free
enzyme and its difficulty in recycling and reuse restrict its wider application, and efficient
enzyme immobilization and bioreactor design are required.In this study, and two
membrane bioreactors were proposed for carbamazepine (CBZ) degradation: the hybrid
membrane system where laccase-immobilized TiO2 nanoparticles were suspended in the feed solution, and the biocatalytic
membrane reactor where laccase was immobilised on TiO2 coated
membrane surface. Using p-coumaric acid as a mediator, efficient CBZ removal (up to 71%) was achieved with the hybrid
membrane reactor. Functionalized TiO2 nanoparticles were further applied to immobilize crude
enzyme extracts from P. ostreatus culture. The resultant biocatalytic particles had comparable performance to the immobilized purified commercial laccase and showed efficient bisphenol-A and CBZ removal in the hybrid
reactor.In addition, a cross-linked carbon nanotubes (CNTs) based
membrane was prepared, which exhibited high effectiveness as support for physical adsorption of laccase. The active laccase coating on CNTs
membrane can be renewed after simple cleaning and re-immobilization. The biocatalytic
membrane also showed substantial improvement in micro-pollutant removal compared with the
membrane having no
enzyme. At last, it is demonstrated that the intramolecular electron transfer within single
enzyme molecule is an important alternative pathway which can be harnessed to generate electricity. By decoupling the redox reaction within laccase, efficient electricity production from unconventional fuels including recalcitrant pollutants and/or toxic organic was obtained in a sole-laccase based enzymatic fuel cell. The intramolecular electron-harnessing concept was also demonstrated with other enzymes, including the power generation during CO2 bioconversion to formate catalysed by formate dehydrogenase. The novel enzymatic power generation is shown to be potentially feasible utilizing wastewater as fuel as well as occurring in tandem with driving bioconversion of chemical feedstock from CO2.
Advisors/Committee Members: Chen, Vicki, Chemical Engineering, Faculty of Engineering, UNSW.
Subjects/Keywords: Micro-pollutant; Laccase; Biocatalytic membrane reactor; Enzymatic fuel cell; Enzyme immobilization
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APA ·
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APA (6th Edition):
Ji, C. (2017). Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Ji, Chao. “Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.” 2017. Doctoral Dissertation, University of New South Wales. Accessed January 22, 2021.
http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true.
MLA Handbook (7th Edition):
Ji, Chao. “Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.” 2017. Web. 22 Jan 2021.
Vancouver:
Ji C. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2021 Jan 22].
Available from: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true.
Council of Science Editors:
Ji C. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true
Georgia Tech
4.
Rogers, Thomas A.
Long-term biocatalyst performance via heuristic and rigorous modeling approaches.
Degree: PhD, Chemical Engineering, 2010, Georgia Tech
URL: http://hdl.handle.net/1853/37194
► The experiments which are required to directly assess the operational stability of thermostable biocatalysts can be time-consuming, troublesome, and, in the context of industry, expensive.…
(more)
▼ The experiments which are required to directly assess the operational stability of thermostable biocatalysts can be time-consuming, troublesome, and, in the context of industry, expensive. In the present work, we develop and validate two methods for quickly estimating the total turnover number (a useful indicator of lifetime productivity) of a biocatalyst for any desired operating temperature. The first method is a heuristic approach, built upon a complete mathematical derivation from first principles, in which the total turnover number can be calculated from two simple biochemical measurements. The second method relies on a single non-isothermal, continuous-mode experiment in conjunction with mathematical modeling to determine the intrinsic deactivation parameters of the biocatalyst. Both methods provide estimates of the total turnover number which are well within one order of magnitude of the values measured directly via isothermal aging tests and therefore are extremely valuable tools in terms of the amount of experimental time eliminated.
Advisors/Committee Members: Andreas Bommarius (Committee Chair), Amyn Teja (Committee Member), Athanassios Sambanis (Committee Member), Roy Daniel (Committee Member), Thomas Orlando (Committee Member).
Subjects/Keywords: Protein stability; Total turnover number; Enzyme membrane reactor; Enzymes; Proteins
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APA (6th Edition):
Rogers, T. A. (2010). Long-term biocatalyst performance via heuristic and rigorous modeling approaches. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/37194
Chicago Manual of Style (16th Edition):
Rogers, Thomas A. “Long-term biocatalyst performance via heuristic and rigorous modeling approaches.” 2010. Doctoral Dissertation, Georgia Tech. Accessed January 22, 2021.
http://hdl.handle.net/1853/37194.
MLA Handbook (7th Edition):
Rogers, Thomas A. “Long-term biocatalyst performance via heuristic and rigorous modeling approaches.” 2010. Web. 22 Jan 2021.
Vancouver:
Rogers TA. Long-term biocatalyst performance via heuristic and rigorous modeling approaches. [Internet] [Doctoral dissertation]. Georgia Tech; 2010. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/1853/37194.
Council of Science Editors:
Rogers TA. Long-term biocatalyst performance via heuristic and rigorous modeling approaches. [Doctoral Dissertation]. Georgia Tech; 2010. Available from: http://hdl.handle.net/1853/37194
Ohio University
5.
Hong, Eock Kee.
Analysis of the hollow fiber membrane reactor using
immobilized enzyme with deactivation.
Degree: MS, Chemical Engineering (Engineering), 1986, Ohio University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1183132380
► The performance of the hollow fiber membrane reactor for several reaction schemes in two different modes of reactor operations is theoretically evaluated. In this…
(more)
▼ The performance of the hollow fiber
membrane
reactor for several reaction schemes in two different modes of
reactor operations is theoretically evaluated. In this
enzyme
reactor, the substrate solution diffuses across the hollow fiber
membrane from the tube side to the shell side where the
enzyme
reaction occurrs to form a product. The product then back-diffuses
into the circulating reactant mixture stream. At high recycle
ratios, this system can be assumed to be a continuous flow stirred
tank
reactor. This mathematical study compares the efficiencies of
the two modes of
reactor operation depending on the
enzyme
deactivation. In Mode I, the flow rate is fixed while the final
exit conversion of the substrate is allowed to decrease with time
due to
enzyme deactivation. In Mode II, the final exit conversion
is fixed while the flow rate is decreased with time to compensate
for the loss of the
enzyme activity. In this work, it is observed
that Mode I
reactor operation is more effective than Mode
II.
Advisors/Committee Members: Dinos, Nicholas (Advisor).
Subjects/Keywords: Engineering, Chemical; Analysis; Hollow Fiber Membrane Reactor; Immobilized Enzyme with Deactivation
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APA (6th Edition):
Hong, E. K. (1986). Analysis of the hollow fiber membrane reactor using
immobilized enzyme with deactivation. (Masters Thesis). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1183132380
Chicago Manual of Style (16th Edition):
Hong, Eock Kee. “Analysis of the hollow fiber membrane reactor using
immobilized enzyme with deactivation.” 1986. Masters Thesis, Ohio University. Accessed January 22, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1183132380.
MLA Handbook (7th Edition):
Hong, Eock Kee. “Analysis of the hollow fiber membrane reactor using
immobilized enzyme with deactivation.” 1986. Web. 22 Jan 2021.
Vancouver:
Hong EK. Analysis of the hollow fiber membrane reactor using
immobilized enzyme with deactivation. [Internet] [Masters thesis]. Ohio University; 1986. [cited 2021 Jan 22].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1183132380.
Council of Science Editors:
Hong EK. Analysis of the hollow fiber membrane reactor using
immobilized enzyme with deactivation. [Masters Thesis]. Ohio University; 1986. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1183132380
. 
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