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Georgia Tech

1. DeSilva, Veronica. Selenium redox cycling; isolation and characterization of a stimulatory component from tissue of loblolly pine for multiplication of somatic embryos; development of an assay to measure l-phenylalanine concentration in blood plasma.

Degree: PhD, Chemistry and Biochemistry, 2007, Georgia Tech

Exogenously supplied organoselenium compounds, capable of propagating a selenium redox cycle, were shown to supplement natural cellular defenses against oxidants generated during biological activity. Phenylaminoalkyl selenides were developed in our laboratory as novel substrate analogs for the enzyme dopamine beta-monooxygenase. Recently, phenylaminoalkyl selenides were found to protect plasmid DNA and Molecular beacons from oxoperoxynitrate – mediated damage by scavenging this oxidant and forming the corresponding selenoxides as the sole selenium – containing products. Rate constants were determined for the reactions of the phenylaminoalkyl selenoxides with GSH at physiological pH and 25 degrees C. The kinetic data obtained in current and previous research was subsequently used in a MatLab simulation, which showed the feasibility of selenium redox cycling by GSH in the presence of a cellular oxidant, oxoperoxynitrate. Loblolly pine (LP, Pinus taeda) is the primary commercial species in southern forests covering 11.7 million hectares. Somatic embryogenesis (SE) is an effective technique to implement production of high value genotypes of LP. SE is a multi-step process, which includes initiation of somatic embryo (SME) growth from tree tissue, maintenance and multiplication of early stage SMEs and the maturation / germination phase. In this work, we isolated a substance from stage 2 or 3 LP female gametophyte (FG) tissue that stimulates early stage SME growth, and characterized this substance as citric acid on the basis of 1H NMR and mass spectrometry. We then demonstrated that topical application of citric acid to SMEs stimulates embryo colony growth at p = 0.05 for 3 of the 5 genotypes tested. Phenylketonuria (PKU) is an autosomal recessive disorder caused by an impaired conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr). A novel assay based on enzymatic - colorimetric methodology (ECA) was developed in order to detect elevated concentrations of L-Phe in undeproteinized plasma of PKU patients via continuous spectrophotometric detection. We report here that L-Phe concentrations in undeproteinized plasma measured using our ECA were comparable to those determined on an amino acid analyzer based on Pearson correlation coefficients and a Bland and Altman comparison. Advisors/Committee Members: Sheldon May (Committee Chair), Nicholas Hud, Stanley Pollock, James Powers, and Gerald Pullman (Committee Members).

Subjects/Keywords: Phenylalanine dehydrogenase; PMS; MTS; Phenylketonuria; Enzymatic colorimetric assay; Citric acid; Loblolly pine; Somatic embryogenesis; GSH; Recycling; Molecular beacon; Phenylaminoalkyl selenides; Plasma amino acid analysis; HPLC; Tandem mass spectrometry; Peroxynitrite; Oxoperoxynitrate; Phenylaminoalkylselenoxides; Comparison; L-phenylalanine; Selenium; Oxidation-reduction reaction; Loblolly pine; Somatic embryogenesis; Phenylalanine; Blood plasma

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APA (6th Edition):

DeSilva, V. (2007). Selenium redox cycling; isolation and characterization of a stimulatory component from tissue of loblolly pine for multiplication of somatic embryos; development of an assay to measure l-phenylalanine concentration in blood plasma. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/29788

Chicago Manual of Style (16th Edition):

DeSilva, Veronica. “Selenium redox cycling; isolation and characterization of a stimulatory component from tissue of loblolly pine for multiplication of somatic embryos; development of an assay to measure l-phenylalanine concentration in blood plasma.” 2007. Doctoral Dissertation, Georgia Tech. Accessed February 27, 2021. http://hdl.handle.net/1853/29788.

MLA Handbook (7th Edition):

DeSilva, Veronica. “Selenium redox cycling; isolation and characterization of a stimulatory component from tissue of loblolly pine for multiplication of somatic embryos; development of an assay to measure l-phenylalanine concentration in blood plasma.” 2007. Web. 27 Feb 2021.

Vancouver:

DeSilva V. Selenium redox cycling; isolation and characterization of a stimulatory component from tissue of loblolly pine for multiplication of somatic embryos; development of an assay to measure l-phenylalanine concentration in blood plasma. [Internet] [Doctoral dissertation]. Georgia Tech; 2007. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1853/29788.

Council of Science Editors:

DeSilva V. Selenium redox cycling; isolation and characterization of a stimulatory component from tissue of loblolly pine for multiplication of somatic embryos; development of an assay to measure l-phenylalanine concentration in blood plasma. [Doctoral Dissertation]. Georgia Tech; 2007. Available from: http://hdl.handle.net/1853/29788

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