You searched for subject:(Enterobacteriaceae)
.
Showing records 1 – 30 of
301 total matches.
◁ [1] [2] [3] [4] [5] … [11] ▶

Universidad Andrés Bello
1.
Valderrama Herrera, Camila Fernanda.
Efecto antimicrobiano de metabolitos secundarios y extractos naturales contra bacterias resistentes a carbapenemicos
.
Degree: 2015, Universidad Andrés Bello
URL: http://repositorio.unab.cl/xmlui/handle/ria/5089
► Las Enterobacterias son una familia amplia y compleja de bacilos Gram negativo causantes de enfermedades infecciosas. Diversos factores como el uso inadecuado de terapia farmacológica…
(more)
▼ Las Enterobacterias son una familia amplia y compleja de bacilos Gram negativo causantes de enfermedades infecciosas. Diversos factores como el uso inadecuado de terapia farmacológica y el abuso de antibióticos sin prescripción médica, entre otras cosas, provocaron el fenómeno conocido como resistencia antimicrobiana.
Las bacterias pueden manifestar variados mecanismos de resistencia, unas de las más importantes son las β-lactamasas, enzimas que tiene la capacidad de modificar la acción de β-lactámicos, antibióticos que intervienen en la síntesis de la pared bacteriana. Dentro de este grupo se encuentran las carbapenemasas, enzimas encargadas de la hidrólisis de los antibióticos carbapenémicos. Estos fármacos constituyen en medicina una opción valiosa en la terapia médica, para el tratamiento de Enterobacterias resistentes a los antibióticos β-lactámicos, sin embargo, actualmente su uso no es efectivo en su totalidad.
Investigaciones recientes indican que terapias alternativas podrían entregar una solución a esta problemática, a través del uso de metabolitos secundarios obtenidos de compuestos naturales, que han demostrado efectos inhibitorios sobre algunas bacterias Gram Positivo. Sin embargo, no se conocen estudios comprobados en Gram Negativo multirresistentes.
Esta investigación tiene como objetivo principal encontrar posibles efectos inhibitorios por parte de metabolitos secundarios obtenidos desde extracto naturales que actúen sobre Enterobacterias multirresistentes. Se pretende obtener y cuantificar mediante Concentraciones Inhibitoria Mínima (CIM) los metabolitos secundarios y extractos naturales con actividad antimicrobiana. Para luego, identificar el mecanismo de acción de tales compuestos.
Se realizara un estudio descriptivo y comparativo con el fin de contrastar, bacterias que presentan mecanismos de resistencia del tipo carbapenemasas y bacterias que no las manifiestan.
Advisors/Committee Members: Villagra, Nicolás (advisor), Cornejo Mora, Alberto (advisor).
Subjects/Keywords: Metabolitos;
Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Valderrama Herrera, C. F. (2015). Efecto antimicrobiano de metabolitos secundarios y extractos naturales contra bacterias resistentes a carbapenemicos
. (Thesis). Universidad Andrés Bello. Retrieved from http://repositorio.unab.cl/xmlui/handle/ria/5089
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Valderrama Herrera, Camila Fernanda. “Efecto antimicrobiano de metabolitos secundarios y extractos naturales contra bacterias resistentes a carbapenemicos
.” 2015. Thesis, Universidad Andrés Bello. Accessed April 15, 2021.
http://repositorio.unab.cl/xmlui/handle/ria/5089.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Valderrama Herrera, Camila Fernanda. “Efecto antimicrobiano de metabolitos secundarios y extractos naturales contra bacterias resistentes a carbapenemicos
.” 2015. Web. 15 Apr 2021.
Vancouver:
Valderrama Herrera CF. Efecto antimicrobiano de metabolitos secundarios y extractos naturales contra bacterias resistentes a carbapenemicos
. [Internet] [Thesis]. Universidad Andrés Bello; 2015. [cited 2021 Apr 15].
Available from: http://repositorio.unab.cl/xmlui/handle/ria/5089.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Valderrama Herrera CF. Efecto antimicrobiano de metabolitos secundarios y extractos naturales contra bacterias resistentes a carbapenemicos
. [Thesis]. Universidad Andrés Bello; 2015. Available from: http://repositorio.unab.cl/xmlui/handle/ria/5089
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
2.
Brooks, Lauren (Lauren Elisabeth).
Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water.
Degree: PhD, Microbiology, 2016, Oregon State University
URL: http://hdl.handle.net/1957/59778
► High counts of fecal indicators, used to signal the potential presence of pathogens associated with untreated waste, result in the classification of water bodies throughout…
(more)
▼ High counts of fecal indicators, used to signal the potential presence of pathogens associated with untreated waste, result in the classification of water bodies throughout the United States as impaired. Nonpoint sources of unknown origin that contribute to fecal contamination make management of impaired waters challenging, as they are difficult to distinguish, and it is thus problematic to correctly target mitigation efforts. Genetic markers used for microbial source tracking provide valuable information by identifying hosts that contribute to fecal loading, but do not provide a method to detect specific sources that contribute to impairment of water bodies. Spatial modelling efforts have been proposed for use in conjunction with fecal indicators and host-specific markers, but have been limited by a lack of adequate modeling for the complex processes that cause indicator decay.
We conducted a quantitative meta-analysis of published decay rate estimates for several common indicators using Bayesian hierarchical linear modeling. The meta-analysis revealed a large amount of variability across studies, including in findings of significance for environmental parameters that impact persistence. Additionally, the meta-analysis revealed gaps in the data for genetic markers, while sufficient data was available for the traditional, culture-based indicators. We determined that temperature was consistently a significant predictor of decay rate estimates for all indicators, but light was only significant for culture-based indicators. We provided synthesized estimates for the selected indicators, but recommend caution in their application for source tracking or quantitative risk assessment due to high variability in parameter estimates and uncertainty in their extension beyond artificial settings.
We compared the decay profiles for general fecal indicators and markers associated with ruminants and cattle. We determined best fitting non-linear models based on information theory and used global model fitting to test for differences in curves for each combination of indicators. Additionally, we investigated the potential of the selected ruminant markers for use in source allocation using the ratio method, based on difference in the observed decay profiles. We found statistical differences between the decay curves of E. coli and all but one genetic marker. The differences across decay profiles suggest caution is necessary when interpreting microbial source tracking results using these markers, as differential decay may result in different findings depending on the marker selected.
We assessed the possibility of studying fecal indicator persistence in a truly open system using simulations. Using the concept of a Continuous-flow Stirred Tank Reactor, we developed an adjustment that can be applied to observed fecal indicator concentrations from an open system so that only loss due to decay is considered. The simulations showed that this adjustment is an effective way to account for loss for this system. However, implementation of…
Advisors/Committee Members: Field, Katharine G. (advisor), Shanks, Orin C. (committee member).
Subjects/Keywords: Fecal Indicators; Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Brooks, L. (. E. (2016). Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/59778
Chicago Manual of Style (16th Edition):
Brooks, Lauren (Lauren Elisabeth). “Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water.” 2016. Doctoral Dissertation, Oregon State University. Accessed April 15, 2021.
http://hdl.handle.net/1957/59778.
MLA Handbook (7th Edition):
Brooks, Lauren (Lauren Elisabeth). “Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water.” 2016. Web. 15 Apr 2021.
Vancouver:
Brooks L(E. Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water. [Internet] [Doctoral dissertation]. Oregon State University; 2016. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/1957/59778.
Council of Science Editors:
Brooks L(E. Novel Experimental Designs and Mathematical Models to Study Fecal Indicator Bacteria Persistence in Surface Water. [Doctoral Dissertation]. Oregon State University; 2016. Available from: http://hdl.handle.net/1957/59778
3.
Rafael Gariglio Clark Xavier.
Filogrupos e fatores de virulência de estirpes de Escherichia coli obtidas do conteúdo uterino e de fezes de cadelas com piometra.
Degree: 2020, Universidade Federal de Minas Gerais; Programa de Pós-Graduação em Ciência Animal; UFMG; Brasil; VET – DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
URL: http://hdl.handle.net/1843/32591
► Submitted by Rafael Xavier ([email protected]) on 2020-02-14T13:36:16Z No. of bitstreams: 1 Dissertação - Rafael Gariglio Clark Xavier.pdf: 913391 bytes, checksum: d60dff5f38b5192fc8ef2ac1225ea185 (MD5)
Approved for entry…
(more)
▼ Submitted by Rafael Xavier ([email protected]) on 2020-02-14T13:36:16Z No. of bitstreams: 1 Dissertação - Rafael Gariglio Clark Xavier.pdf: 913391 bytes, checksum: d60dff5f38b5192fc8ef2ac1225ea185 (MD5)
Approved for entry into archive by Eliane Araujo ([email protected]) on 2020-02-20T13:25:57Z (GMT) No. of bitstreams: 1 Dissertação - Rafael Gariglio Clark Xavier.pdf: 913391 bytes, checksum: d60dff5f38b5192fc8ef2ac1225ea185 (MD5)
Made available in DSpace on 2020-02-20T15:07:57Z (GMT). No. of bitstreams: 1 Dissertação - Rafael Gariglio Clark Xavier.pdf: 913391 bytes, checksum: d60dff5f38b5192fc8ef2ac1225ea185 (MD5) Previous issue date: 2020-02-06
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
Diversos agentes bacterianos estão associados com a ocorrência de piometra em cadelas, porém o patotipo de E. coli patogênica extraintestinal (ExPEC) é o mais frequente, estando associado a 82 a 100% dos casos. O objetivo do
presente estudo foi determinar a frequência dos grupos filogenéticos e fatores de virulência associados a estirpes de E. coli isoladas de conteúdo uterino e fezes de 55 cadelas, totalizando 110 amostras clínicas. Um total de 225 estirpes de E. coli foram isoladas e divididas em três categorias: 90 (40%) isolados do conteúdo uterino, 75 (33,3%) isolados das fezes de cadelas com piometra por E. coli e 60 (26,6%) das fezes de cadelas em que a piometra não foi causada por E. coli. No conteúdo uterino, predominaram estirpes de E. coli pertencentes ao filogrupo B2 e positivas para os genes de fatores de virulência associados a adesão (papC, papG e sfaS) e produção de toxinas (hlyA, cnf-1 e usp). Similarmente, elevadas frequências de estirpes pertencentes ao filogrupo B2 foram identificadas nas fezes de cadelas com piometra por E. coli, enquanto cadelas com infecção uterina não provocada por E. coli eliminaram mais frequentemente estirpes do filogrupo B1. Os fatores de virulência fímbria
tipo P (papC e papG) e proteína uropatogênica específica (usp), previamente associados à piometra em estudos anteriores, foram identificados com frequência superior em fezes de cadelas com piometra por E. coli (p ≤ 0,05) . O presente estudo demonstra, portanto, que estirpes ExPEC do filogrupo B2 são comumente encontradas causando piometra em cães. Em adição, a elevada frequência de estirpes do filogrupo B2 e carreadoras dos genes de virulência papC, papG e usp nas fezes de cadelas com piometra associada a E. coli sugere que a colonização por essas estirpes possa estar relacionada com um maior risco de piometra por E. coli nesses animais.
Several bacterial agents are associated with the occurrence of pyometra in female dogs, but the extraintestinal pathogenic E. coli (ExPEC) pathotype is the most common, being associated with 82 to 100% of the cases. The aim of the present study was to determine the frequency of phylogenetic groups and virulence factors associated with isolated E.
coli strains with uterine content and feces of 55 female dogs, totalizing 110 clinical samples. A total of 225 E.…
Advisors/Committee Members: Rodrigo Otávio Silveira Silva, Patricia Maria Colleto Freitas, Fernanda Morcatti Coura.
Subjects/Keywords: Enterobacteriaceae; cães; microbiota
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Xavier, R. G. C. (2020). Filogrupos e fatores de virulência de estirpes de Escherichia coli obtidas do conteúdo uterino e de fezes de cadelas com piometra. (Masters Thesis). Universidade Federal de Minas Gerais; Programa de Pós-Graduação em Ciência Animal; UFMG; Brasil; VET – DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA. Retrieved from http://hdl.handle.net/1843/32591
Chicago Manual of Style (16th Edition):
Xavier, Rafael Gariglio Clark. “Filogrupos e fatores de virulência de estirpes de Escherichia coli obtidas do conteúdo uterino e de fezes de cadelas com piometra.” 2020. Masters Thesis, Universidade Federal de Minas Gerais; Programa de Pós-Graduação em Ciência Animal; UFMG; Brasil; VET – DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA. Accessed April 15, 2021.
http://hdl.handle.net/1843/32591.
MLA Handbook (7th Edition):
Xavier, Rafael Gariglio Clark. “Filogrupos e fatores de virulência de estirpes de Escherichia coli obtidas do conteúdo uterino e de fezes de cadelas com piometra.” 2020. Web. 15 Apr 2021.
Vancouver:
Xavier RGC. Filogrupos e fatores de virulência de estirpes de Escherichia coli obtidas do conteúdo uterino e de fezes de cadelas com piometra. [Internet] [Masters thesis]. Universidade Federal de Minas Gerais; Programa de Pós-Graduação em Ciência Animal; UFMG; Brasil; VET – DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA; 2020. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/1843/32591.
Council of Science Editors:
Xavier RGC. Filogrupos e fatores de virulência de estirpes de Escherichia coli obtidas do conteúdo uterino e de fezes de cadelas com piometra. [Masters Thesis]. Universidade Federal de Minas Gerais; Programa de Pós-Graduação em Ciência Animal; UFMG; Brasil; VET – DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA; 2020. Available from: http://hdl.handle.net/1843/32591

Ruhr Universität Bochum
4.
Topal, Hüsnü.
Biochemische und strukturelle Charakterisierung von
Adenylylzyklasen aus humanpathogenen Mikroorganismen.
Degree: 2011, Ruhr Universität Bochum
URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-31861
► Adenylylzyklasen (ACs) katalysieren die Bildung des sekundären Botenstoffs cAMP und spielen in der Pathogenität von Mikroorganismen eine wichtige Rolle. In der vorliegenden Arbeit wurden ACs…
(more)
▼ Adenylylzyklasen (ACs) katalysieren die Bildung des
sekundären Botenstoffs cAMP und spielen in der Pathogenität von
Mikroorganismen eine wichtige Rolle. In der vorliegenden Arbeit
wurden ACs aus verschiedenen Pathogenen biochemisch und strukturell
charakterisiert. Die Röntgenstruktur-Analyse der AC CyaB aus P.
aeruginosa weist eine für Klasse III ACs typische Tertiärstruktur
auf. Die Modellierung des aktiven Zentrums von CyaB zeigt geringe
Unterschiede zu den bekannten ACs und erklärt ihre schwächere
Hemmung durch bekannte Inhibitoren. Einzelne Komponenten des
Chp-Virulenz- Systems von P. aeruginosa erhöhen den cAMP-Spiegel.
Die Lage und Funktion der identifizierten Reste, die an der CyaB-
Regulierung durch das Chp-Virulenz System beteiligt sind, wurden
anhand der gelösten Kristallstruktur von CyaB analysiert und
veranschaulichen einen neuen Regulationsmechanismus in der
komplexen Signalkaskade der Produktion von Virulenzfaktoren bei P.
aeruginosa.
Advisors/Committee Members: Biologie.
Subjects/Keywords: Struktur; Virulenz; Enterobacteriaceae; Katalyse;
Inhibition
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Topal, H. (2011). Biochemische und strukturelle Charakterisierung von
Adenylylzyklasen aus humanpathogenen Mikroorganismen. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-31861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Topal, Hüsnü. “Biochemische und strukturelle Charakterisierung von
Adenylylzyklasen aus humanpathogenen Mikroorganismen.” 2011. Thesis, Ruhr Universität Bochum. Accessed April 15, 2021.
http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-31861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Topal, Hüsnü. “Biochemische und strukturelle Charakterisierung von
Adenylylzyklasen aus humanpathogenen Mikroorganismen.” 2011. Web. 15 Apr 2021.
Vancouver:
Topal H. Biochemische und strukturelle Charakterisierung von
Adenylylzyklasen aus humanpathogenen Mikroorganismen. [Internet] [Thesis]. Ruhr Universität Bochum; 2011. [cited 2021 Apr 15].
Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-31861.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Topal H. Biochemische und strukturelle Charakterisierung von
Adenylylzyklasen aus humanpathogenen Mikroorganismen. [Thesis]. Ruhr Universität Bochum; 2011. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-31861
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria
5.
[No author].
Epiphytic and endophytic members of the
Enterobacteriaceae associated with healthy Eucalyptus
trees
.
Degree: 2009, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-07092008-102232/
► Studies presented in this thesis, highlights the importance of determining whether members of the Enterobacteriaceae can be associated with plants as epi- and endophytes. In…
(more)
▼ Studies presented in this thesis, highlights the
importance of determining whether members of the
Enterobacteriaceae
can be associated with plants as epi- and endophytes. In
particular, whether the causal agent of blight and die-back of
Eucalyptus can survive both epi- and endophytically onlin its host
as well as in weeds grown in close proximity to these hosts. This
knowledge allows one a better understanding of the etiology and
epidemiology of this disease. Appropriate management strategies can
now be provided and the impact of the disease lessened in the
nursery environment Chapter One presents an evaluation of the
potential importance of
Enterobacteriaceae as epi- and endophytes
on/in plants. Some information is known about the epi- and
endophytes associated with economically important agricultural
crops. This information is largely lacking for tree species,
especially those grown for commercial forestry purposes. Many
Enterobacteriaceae occur both epi- and endophytically onlin plants
including Pantoea ananatis. This pathogen is known to occur
epiphytically on weeds as well as on its hosts where under ideal
environmental conditions it is capable of causing disease symptoms.
As an endophyte, P. ananatis occurs in dune grass where it fixes
nitrogen and in sweet potato where it is believed to protect the
plant against fungal pathogens. Chapter Two analyses healthy
leaves, both young and mature, removed from various clones of the
hydrid, E. grandis x E. nitens, for the presence of bacterial epi-
and endophytes.
Enterobacteriaceae were also isolated and these
included Pantoea spp. and Enterobacter spp. P. ananatis was
isolated both epi- and endophytically onlin healthy Eucalyptus
tissue as well as from leaves removed from weeds growing in close
proximity to the diseased plants. This thesis clearly indicates
that P. ananatis can occur both epi- and endophytically in healthy
Eucalyptus tissue. The movement of planting material into new
environments where bacterial blight and die-back does not occur
should be restricted. Irrigation practices in nurseries should be
reviewed to prevent the accumulation of water on the plant surface
which will allow for entry of the pathogen into the host through
natural openings. Another management strategy that must also be
recommended is that stringent weed control be implemented in the
nursery environment.
Advisors/Committee Members: Prof K A Coutinho (advisor), Prof S N Venter (advisor).
Subjects/Keywords: Healthy eucalyptus trees;
Enterobacteriaceae;
UCTD
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2009). Epiphytic and endophytic members of the
Enterobacteriaceae associated with healthy Eucalyptus
trees
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07092008-102232/
Chicago Manual of Style (16th Edition):
author], [No. “Epiphytic and endophytic members of the
Enterobacteriaceae associated with healthy Eucalyptus
trees
.” 2009. Masters Thesis, University of Pretoria. Accessed April 15, 2021.
http://upetd.up.ac.za/thesis/available/etd-07092008-102232/.
MLA Handbook (7th Edition):
author], [No. “Epiphytic and endophytic members of the
Enterobacteriaceae associated with healthy Eucalyptus
trees
.” 2009. Web. 15 Apr 2021.
Vancouver:
author] [. Epiphytic and endophytic members of the
Enterobacteriaceae associated with healthy Eucalyptus
trees
. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Apr 15].
Available from: http://upetd.up.ac.za/thesis/available/etd-07092008-102232/.
Council of Science Editors:
author] [. Epiphytic and endophytic members of the
Enterobacteriaceae associated with healthy Eucalyptus
trees
. [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-07092008-102232/

University of Pretoria
6.
Makhado, Ndivhuho
Agnes.
Epiphytic and
endophytic members of the Enterobacteriaceae associated with
healthy Eucalyptus trees.
Degree: Microbiology and Plant
Pathology, 2009, University of Pretoria
URL: http://hdl.handle.net/2263/26148
► Studies presented in this thesis, highlights the importance of determining whether members of the Enterobacteriaceae can be associated with plants as epi- and endophytes. In…
(more)
▼ Studies presented in this thesis, highlights the
importance of determining whether members of the
Enterobacteriaceae
can be associated with plants as epi- and endophytes. In
particular, whether the causal agent of blight and die-back of
Eucalyptus can survive both epi- and endophytically onlin its host
as well as in weeds grown in close proximity to these hosts. This
knowledge allows one a better understanding of the etiology and
epidemiology of this disease. Appropriate management strategies can
now be provided and the impact of the disease lessened in the
nursery environment Chapter One presents an evaluation of the
potential importance of
Enterobacteriaceae as epi- and endophytes
on/in plants. Some information is known about the epi- and
endophytes associated with economically important agricultural
crops. This information is largely lacking for tree species,
especially those grown for commercial forestry purposes. Many
Enterobacteriaceae occur both epi- and endophytically onlin plants
including Pantoea ananatis. This pathogen is known to occur
epiphytically on weeds as well as on its hosts where under ideal
environmental conditions it is capable of causing disease symptoms.
As an endophyte, P. ananatis occurs in dune grass where it fixes
nitrogen and in sweet potato where it is believed to protect the
plant against fungal pathogens. Chapter Two analyses healthy
leaves, both young and mature, removed from various clones of the
hydrid, E. grandis x E. nitens, for the presence of bacterial epi-
and endophytes.
Enterobacteriaceae were also isolated and these
included Pantoea spp. and Enterobacter spp. P. ananatis was
isolated both epi- and endophytically onlin healthy Eucalyptus
tissue as well as from leaves removed from weeds growing in close
proximity to the diseased plants. This thesis clearly indicates
that P. ananatis can occur both epi- and endophytically in healthy
Eucalyptus tissue. The movement of planting material into new
environments where bacterial blight and die-back does not occur
should be restricted. Irrigation practices in nurseries should be
reviewed to prevent the accumulation of water on the plant surface
which will allow for entry of the pathogen into the host through
natural openings. Another management strategy that must also be
recommended is that stringent weed control be implemented in the
nursery environment.
Advisors/Committee Members: Prof K A Coutinho (advisor), Prof S N Venter (coadvisor).
Subjects/Keywords: Healthy
eucalyptus trees;
Enterobacteriaceae;
UCTD
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Makhado, N. (2009). Epiphytic and
endophytic members of the Enterobacteriaceae associated with
healthy Eucalyptus trees. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/26148
Chicago Manual of Style (16th Edition):
Makhado, Ndivhuho. “Epiphytic and
endophytic members of the Enterobacteriaceae associated with
healthy Eucalyptus trees.” 2009. Masters Thesis, University of Pretoria. Accessed April 15, 2021.
http://hdl.handle.net/2263/26148.
MLA Handbook (7th Edition):
Makhado, Ndivhuho. “Epiphytic and
endophytic members of the Enterobacteriaceae associated with
healthy Eucalyptus trees.” 2009. Web. 15 Apr 2021.
Vancouver:
Makhado N. Epiphytic and
endophytic members of the Enterobacteriaceae associated with
healthy Eucalyptus trees. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/2263/26148.
Council of Science Editors:
Makhado N. Epiphytic and
endophytic members of the Enterobacteriaceae associated with
healthy Eucalyptus trees. [Masters Thesis]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/26148

University of Nairobi
7.
Njiru, Simon K.
Antimicrobial Susceptibility Patterns Of Enterobacteriaceae Isolated From Urine Samples At The Kenyatta National Hospital Microbiology Laboratory
.
Degree: 2013, University of Nairobi
URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/61637
► Background: Antimicrobial resistance is an increasingly emerging problem worldwide and is a critical challenge for infectious diseases management around the world.Data from the National Healthcare…
(more)
▼ Background: Antimicrobial resistance is an increasingly emerging problem worldwide and is a critical challenge for infectious diseases management around the world.Data from the National Healthcare Safety Network indicate that gram-negative bacteria are responsible for more than 30% of hospital-acquired infections and more than 40% of infections in patients in intensive care units. These infections are difficult to manage translating to a higher rate of morbidity and mortality as well as prolonged length of hospital and intensive care unit stay as well as increased expenses for the healthcare systems.
Methods: This was a hospital based cross sectional descriptive survey aimed at identifying antimicrobial susceptibility patterns of the Enterobacteriaceae isolated from urine sampled from both inpatients and outpatients in KNH microbiology laboratory in the period January 2012 to December 2012.Data were retrieved from the archives.A coded form was used to abstract the data. The study was approved by the KNH / U.o.N Ethics and Research Committee.
Results: In this study, among both sexes tested, incidence of Enterobacteriaceae infections was higher in females (56%) than males (44%). Enterobacteriaceae isolated were: E.coli (46%), Klebsiella spp. (19.5%), Citrobacter spp. (15.9%), Proteus spp. (7.1%), Enterobacter spp. (5.8%), Acinetobacter spp. (5.5%). Tested Carbapenems were found to have better activity against majority of the isolates. ESBL production was found to be high (>60%).
Conclusion: E. coli was the most common isolate. Tested carbapenems were more effective, than other drugs, against all the isolates. Proteus spp. were the prevalent ESBL producers.
Acinetobacter spp. and E. coli showed higher and lower resistance respectively to carbapenems
Subjects/Keywords: Antimicrobial susceptibility patterns;
Enterobacteriaceae.
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Njiru, S. K. (2013). Antimicrobial Susceptibility Patterns Of Enterobacteriaceae Isolated From Urine Samples At The Kenyatta National Hospital Microbiology Laboratory
. (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/61637
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Njiru, Simon K. “Antimicrobial Susceptibility Patterns Of Enterobacteriaceae Isolated From Urine Samples At The Kenyatta National Hospital Microbiology Laboratory
.” 2013. Thesis, University of Nairobi. Accessed April 15, 2021.
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/61637.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Njiru, Simon K. “Antimicrobial Susceptibility Patterns Of Enterobacteriaceae Isolated From Urine Samples At The Kenyatta National Hospital Microbiology Laboratory
.” 2013. Web. 15 Apr 2021.
Vancouver:
Njiru SK. Antimicrobial Susceptibility Patterns Of Enterobacteriaceae Isolated From Urine Samples At The Kenyatta National Hospital Microbiology Laboratory
. [Internet] [Thesis]. University of Nairobi; 2013. [cited 2021 Apr 15].
Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/61637.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Njiru SK. Antimicrobial Susceptibility Patterns Of Enterobacteriaceae Isolated From Urine Samples At The Kenyatta National Hospital Microbiology Laboratory
. [Thesis]. University of Nairobi; 2013. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/61637
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vrije Universiteit Amsterdam
8.
Overdevest, I.T.M.A.
Extended-spectrum B-lactamase producing Enterobacteriaceae:: diagnostics and epidemiology
.
Degree: 2015, Vrije Universiteit Amsterdam
URL: http://hdl.handle.net/1871/52562
Subjects/Keywords: ESBL;
Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Overdevest, I. T. M. A. (2015). Extended-spectrum B-lactamase producing Enterobacteriaceae:: diagnostics and epidemiology
. (Doctoral Dissertation). Vrije Universiteit Amsterdam. Retrieved from http://hdl.handle.net/1871/52562
Chicago Manual of Style (16th Edition):
Overdevest, I T M A. “Extended-spectrum B-lactamase producing Enterobacteriaceae:: diagnostics and epidemiology
.” 2015. Doctoral Dissertation, Vrije Universiteit Amsterdam. Accessed April 15, 2021.
http://hdl.handle.net/1871/52562.
MLA Handbook (7th Edition):
Overdevest, I T M A. “Extended-spectrum B-lactamase producing Enterobacteriaceae:: diagnostics and epidemiology
.” 2015. Web. 15 Apr 2021.
Vancouver:
Overdevest ITMA. Extended-spectrum B-lactamase producing Enterobacteriaceae:: diagnostics and epidemiology
. [Internet] [Doctoral dissertation]. Vrije Universiteit Amsterdam; 2015. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/1871/52562.
Council of Science Editors:
Overdevest ITMA. Extended-spectrum B-lactamase producing Enterobacteriaceae:: diagnostics and epidemiology
. [Doctoral Dissertation]. Vrije Universiteit Amsterdam; 2015. Available from: http://hdl.handle.net/1871/52562

Michigan State University
9.
Bordt, Dale Emil.
The influence of several factors on the action of penicillin against some menbers of the Enterobacteriaceae.
Degree: PhD, 1955, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:41308
Subjects/Keywords: Enterobacteriaceae; Penicillin
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bordt, D. E. (1955). The influence of several factors on the action of penicillin against some menbers of the Enterobacteriaceae. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:41308
Chicago Manual of Style (16th Edition):
Bordt, Dale Emil. “The influence of several factors on the action of penicillin against some menbers of the Enterobacteriaceae.” 1955. Doctoral Dissertation, Michigan State University. Accessed April 15, 2021.
http://etd.lib.msu.edu/islandora/object/etd:41308.
MLA Handbook (7th Edition):
Bordt, Dale Emil. “The influence of several factors on the action of penicillin against some menbers of the Enterobacteriaceae.” 1955. Web. 15 Apr 2021.
Vancouver:
Bordt DE. The influence of several factors on the action of penicillin against some menbers of the Enterobacteriaceae. [Internet] [Doctoral dissertation]. Michigan State University; 1955. [cited 2021 Apr 15].
Available from: http://etd.lib.msu.edu/islandora/object/etd:41308.
Council of Science Editors:
Bordt DE. The influence of several factors on the action of penicillin against some menbers of the Enterobacteriaceae. [Doctoral Dissertation]. Michigan State University; 1955. Available from: http://etd.lib.msu.edu/islandora/object/etd:41308
10.
Jones, Caron.
Molecular epidemiology of extended-spectrum β-lactamase (ESBL) carrying Enterobacteriaceae at ABM University Health Board.
Degree: PhD, 2012, Swansea University
URL: https://cronfa.swan.ac.uk/Record/cronfa42241
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678423
► Extended-spectrum beta-lactamases (ESBL) mediate resistance to 3rd generation cephalosporins and aztreonam in Enterobacteriaceae and pose major clinical problems. Screened Enterobacteriaceae were collected from PHW Microbiology…
(more)
▼ Extended-spectrum beta-lactamases (ESBL) mediate resistance to 3rd generation cephalosporins and aztreonam in Enterobacteriaceae and pose major clinical problems. Screened Enterobacteriaceae were collected from PHW Microbiology ABM Swansea laboratory and were demonstrated phenotypically to be ESBL- producers by BSAC methods. Isolates were identified using the BD Phoenix Automated system and Bruker Daltonics MALDI Biotyper. 138 isolates were genetically defined as ESBL-producers (103 Escherichia coli, 32 Klebsiella spp., 2 Enterobacter cloacae and 1 Citrobacter freundii) and 4 isolates (2 E. coli, 1 Enterobacter cloacae and 1 Morganella morganii) were genetically confirmed as AmpC-producers. PCR analysis revealed that the most prevalent ESBLs were CTX-M (n=133), predominantly Group 1 (n=128), of which 51% (66/128) contained the I526-CTX- M-15 link region, which is characteristic for epidemic E. coli strain A. PFGE confirmed that these isolates had a clonal relatedness to epidemic E. coli strain A. Allele-specific PCR revealed that all E. coli positive for I526-CTX-M-15 belonged to clone 025b-ST131 (found internationally), which has a high virulence potential and encompasses diverse PFGE patterns. With the molecular epidemiology established; the sensitivity and performance of phenotypic screening and confirmatory assays were analysed so that optimal strategies to handle difficult-to-identify ESBL resistance traits could be determined. In this study, the sensitivity of ESBL screening increased to 100% when ceftazidime was used alongside cefpodoxime. Isolates harbouring ESBL genes are often difficult to treat, as options are greatly limited. Susceptibility to various well-established antibiotics, along with temocillin and tigecycline, were investigated. Temocillin and tigecycline were effective against 98% and 89% of all isolates tested. The carbapenems were the most active antibiotics with 100% of isolates susceptible to imipenem and meropenem and 99% susceptible to ertapenem. Biofilm production in E. coli was also investigated. The pgaABCD gene locus was detected in all ESBL and AmpC-producing E. coli isolates (n=105); however, only 38% of these produced a phenotypic biofilm.
Subjects/Keywords: 610.73; Molecular epidemiology; Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Jones, C. (2012). Molecular epidemiology of extended-spectrum β-lactamase (ESBL) carrying Enterobacteriaceae at ABM University Health Board. (Doctoral Dissertation). Swansea University. Retrieved from https://cronfa.swan.ac.uk/Record/cronfa42241 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678423
Chicago Manual of Style (16th Edition):
Jones, Caron. “Molecular epidemiology of extended-spectrum β-lactamase (ESBL) carrying Enterobacteriaceae at ABM University Health Board.” 2012. Doctoral Dissertation, Swansea University. Accessed April 15, 2021.
https://cronfa.swan.ac.uk/Record/cronfa42241 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678423.
MLA Handbook (7th Edition):
Jones, Caron. “Molecular epidemiology of extended-spectrum β-lactamase (ESBL) carrying Enterobacteriaceae at ABM University Health Board.” 2012. Web. 15 Apr 2021.
Vancouver:
Jones C. Molecular epidemiology of extended-spectrum β-lactamase (ESBL) carrying Enterobacteriaceae at ABM University Health Board. [Internet] [Doctoral dissertation]. Swansea University; 2012. [cited 2021 Apr 15].
Available from: https://cronfa.swan.ac.uk/Record/cronfa42241 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678423.
Council of Science Editors:
Jones C. Molecular epidemiology of extended-spectrum β-lactamase (ESBL) carrying Enterobacteriaceae at ABM University Health Board. [Doctoral Dissertation]. Swansea University; 2012. Available from: https://cronfa.swan.ac.uk/Record/cronfa42241 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678423

Massey University
11.
Kim, Caroline Chae-hyun.
Pectin degradation and metabolism in Monoglobus pectinilyticus 14T from human faeces.
Degree: PhD, Microbiology, 2017, Massey University
URL: http://hdl.handle.net/10179/14065
► Pectin is a conspicuous plant polysaccharide, comprising one third of the dry weight of dietary fibre in common vegetables and fruit. Although pectin is almost…
(more)
▼ Pectin is a conspicuous plant polysaccharide, comprising one third of the dry weight of dietary fibre in common vegetables and fruit. Although pectin is almost completely digested by the human gut microbiota, few bacterial species are known to possess a comprehensive glycobiome to challenge the structurally complex pectin. The current understanding of the colonic degradation of pectin is incomplete, as the knowledge has almost exclusively derived from studying the sequestration system of Bacteroides spp. Here I report the isolation and characterization of Monoglobus pectinilyticus, and the sequencing of its genome which so far encodes the most pectin-specialized repertoire of carbohydrate active enzymes (CAZymes) found from the human gut. M. pectinilyticus also possesses an extracellular pectin degradation system consisting of novel protein constituents which did not find significant sequence homology and functional matches using the most up-to-date nucleotide and protein sequence databases. Proteome analysis of M. pectinilyticus using iTRAQ quantification revealed that pectin-degrading CAZymes and the potential constituents of the novel pectin degradation system were differentially up-regulated in response to the availability of pectin. Finally, using quantitative PCR, a positive correlation was observed between the prevalence of M. pectinilyticus and the consumption of fibre, vegetables, and pectin in individuals living in NZ. The discovery of M. pectinilyticus may add a new layer of complexity onto our interpretation of the colonic pectin degradation by presenting a system highly relevant to the pectin-rich diet of humans, and by suggesting a possibility outside the established paradigms of microbial polysaccharide degradation. The presence of M. pectinilyticus and the related uncultured bacteria in the gastrointestinal systems of humans and animals indicated that the organisms of this lineage are frequent terrestrial gut commensals, prompting an investigation into the genomic and molecular properties underlying their carbohydrate degradation potentials.
Subjects/Keywords: Enterobacteriaceae;
Feces|xMicrobiology;
Pectin
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kim, C. C. (2017). Pectin degradation and metabolism in Monoglobus pectinilyticus 14T from human faeces. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/14065
Chicago Manual of Style (16th Edition):
Kim, Caroline Chae-hyun. “Pectin degradation and metabolism in Monoglobus pectinilyticus 14T from human faeces.” 2017. Doctoral Dissertation, Massey University. Accessed April 15, 2021.
http://hdl.handle.net/10179/14065.
MLA Handbook (7th Edition):
Kim, Caroline Chae-hyun. “Pectin degradation and metabolism in Monoglobus pectinilyticus 14T from human faeces.” 2017. Web. 15 Apr 2021.
Vancouver:
Kim CC. Pectin degradation and metabolism in Monoglobus pectinilyticus 14T from human faeces. [Internet] [Doctoral dissertation]. Massey University; 2017. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/10179/14065.
Council of Science Editors:
Kim CC. Pectin degradation and metabolism in Monoglobus pectinilyticus 14T from human faeces. [Doctoral Dissertation]. Massey University; 2017. Available from: http://hdl.handle.net/10179/14065

The Ohio State University
12.
Mollenkopf, Dixie Francis.
Epidemiology of ß-lactamase-producing Enterobacteriaceae in
Humans and Livestock.
Degree: PhD, Comparative and Veterinary Medicine, 2017, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1505147339870873
► Carbapenems have the broadest spectrum of the large ß-lactam antimicrobials and have been reserved as a “drug of last resort” against invasive Gram-positive and Gram-negative…
(more)
▼ Carbapenems have the broadest spectrum of the large
ß-lactam antimicrobials and have been reserved as a “drug of last
resort” against invasive Gram-positive and Gram-negative human
infections. The increasing prevalence of complicated MDR infections
involving extended spectrum (ESßL) and AmpC ß-lactamases has
triggered the increasing need for carbapenem use. The emergence of
carbapenemase-producing Enterobactericeae was described as “the end
of the antibiotic era” as these potential pathogens harbor
highly-mobile genetic elements that confer resistance to our most
critically important drugs.In the US, nontyphoidal Salmonella are a
common foodborne zoonotic pathogen causing gastroenteritis. MDR
invasive Salmonella infections mediated by ESßL or AmpC genotypes
are more likely to require carbapenem therapy compared to
susceptible infections. Of 571 isolates, we characterized 44
blaCMY-2-bearing Salmonella that resulted from 5,050 individual
cattle fecal samples from 68 large (1,000+ head capacity) US
feedlots participating in the NAHMS Beef Feedlot 2011 study, and
assessed risk factors for blaCMY-2 carriage. Cultured without
antimicrobial selection, the isolates represented eight serotypes
and carried the blaCMY-2/IncA/C gene/plasmid combination with most
expressing the penta-resistance (ACSSuT) phenotype. Cattle fed
chlortetracycline in their diet and heavier weight cattle were less
likely to carry Salmonella with blaCMY-2. In contrast, cattle fed
the macrolide feed additive tylosin and cattle in pens with
increasing numbers of dairy cattle were more likely to harbor
blaCMY-bearing Salmonella. To determine the prevalence of foodborne
resistance mechanisms, we screened human diarrheic stool samples
submitted for Clostridium difficle culture from patients of The
Ohio State University Wexner Medical Center (OSUWMC) to estimate
the frequency of carriage of ESßL- and AmpC- as well as
carbapenemase-producing enteric bacteria. The 692 deidentified
samples received between July and December 2013 were cultured using
selective media to detect the resistant phenotypes. Our selective
culture yielded 184 isolates (26.6 %) with reduced susceptibility
to cefotaxime. Of these, 46 (6.7%) samples harbored commensal
isolates carrying the AmpC blaCMY. Another 21 (3.0%) samples
produced isolates harboring the ESBL blaCTX-M: 19 carrying CTX-M-15
and 2 with CTX-M-27. Additionally, 13 samples (1.9 %) produced
Enterobacteriaceae or Pseudomonas spp. resistant to carbapenems. Of
these, whole genome sequencing identified a prominent CRE strain,
sequence type ST258, K. pneumoniae harboring blaKPC-3 and a second
K. pneumoniae carrying blaNDM-1 ST1602 which had not been
previously reported. Reporting the first mobile carbapenemase,
blaIMP-64 on an IncQ1 plasmid, in US livestock, we followed a
cohort of 350+ pigs from late sow gestation to the final finishing
phase in order to better understand the maintenance of this rare
resistance genotype in a large farrow-to-finish swine operation.
Environmental and fecal samples were collected during 8 visits…
Advisors/Committee Members: Wittum, Thomas (Advisor).
Subjects/Keywords: Epidemiology; Antimicrobial resistance, Enterobacteriaceae,
Carbapenemase
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mollenkopf, D. F. (2017). Epidemiology of ß-lactamase-producing Enterobacteriaceae in
Humans and Livestock. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1505147339870873
Chicago Manual of Style (16th Edition):
Mollenkopf, Dixie Francis. “Epidemiology of ß-lactamase-producing Enterobacteriaceae in
Humans and Livestock.” 2017. Doctoral Dissertation, The Ohio State University. Accessed April 15, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1505147339870873.
MLA Handbook (7th Edition):
Mollenkopf, Dixie Francis. “Epidemiology of ß-lactamase-producing Enterobacteriaceae in
Humans and Livestock.” 2017. Web. 15 Apr 2021.
Vancouver:
Mollenkopf DF. Epidemiology of ß-lactamase-producing Enterobacteriaceae in
Humans and Livestock. [Internet] [Doctoral dissertation]. The Ohio State University; 2017. [cited 2021 Apr 15].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1505147339870873.
Council of Science Editors:
Mollenkopf DF. Epidemiology of ß-lactamase-producing Enterobacteriaceae in
Humans and Livestock. [Doctoral Dissertation]. The Ohio State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1505147339870873
13.
Marqueti, Antonio Carlos [UNESP].
Próteses totais removíveis como reservatório de microrganismos oportunistas.
Degree: 2011, Universidade Estadual Paulista (UNESP)
URL: http://hdl.handle.net/11449/102338
► Made available in DSpace on 2014-06-11T19:32:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-23Bitstream added on 2014-06-13T21:03:19Z : No. of bitstreams: 1 marqueti_ac_dr_araca.pdf: 258633…
(more)
▼ Made available in DSpace on 2014-06-11T19:32:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-23Bitstream added on 2014-06-13T21:03:19Z : No. of bitstreams: 1 marqueti_ac_dr_araca.pdf: 258633 bytes, checksum: 711d6e5f7bcc5a0600ade1c21b730a84 (MD5)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Este estudo avaliou a ocorrência de leveduras do gênero Candida sp além dos principais microrganismos periodontopatogênicos e enterobactérias na saliva, em mucosa e no biofilme aderido à prótese total, correlacionando com aspectos clínicos e condição de higiene bucal de 90 indivíduos edêntulos e portadores de prótese total, por meio de métodos moleculares (PCR). Espécimes clínicos intrabucais foram coletados desses indivíduos após avaliação das condições sócio-econômicas e comportamentais. A microbiota bucal dos pacientes foi caracterizada por meio da obtenção de amostras de biofilme aderido às próteses totais, mucosa e
saliva, as quais foram processadas, por meio de PCR. As inter-relações entre os diferentes microrganismos foram determinadas por meio dos testes de Qui-quadrado e Mann- Whitney. Verificaram-se diferenças na ocorrência de Prevotella intermedia e Enterobacteriaceae na saliva dos pacientes edêntulos, o mesmo ocorrendo com Enterobacteriaceae, Camphylobacter rectus e gênero Pseudomonas no biofilme aderido às próteses totais. As condições de higiene bucal e estado de conservação da prótese total precários favoreceram a ocorrência de leveduras do tipo Candida sp, em especial Candida albicans, em níveis estatisticamente significante nas amostras de mucosa e biofilme aderido à prótese total, tornando este dispositivo protético um potencial reservatório de leveduras e bactérias entéricas que podem ser de relevância na patogênese das infecções oportunistas
The aim of this study was to evaluate the occurrence of major periodontopathogenic microorganisms and Enterobacteriaceae and biofilm
adhered to the denture, mucosa and saliva in 90 edentulous subjects with complete dentures, using molecular methods (PCR). Clinical specimens were collected from these individuals after assessing the socio-economic circumstances and behavioral. The oral microbiota of patients was characterized by obtaining samples of the biofilm adhered to the dental prothesis and saliva, for detection of major pathogens using PCR. The possibility of inter-relationships between different microorganisms was determined using the chi-square test and Mann-Whitney test. There were differences in the occurrence of Prevotella intermedia and Enterobacteriaceae in the saliva of edentulous patients, likewise, Enterobacteriaceae, Pseudomonas, Camphylobacter rectus and the biofilm attached to denture patients. The conditions for oral hygiene and stat of preservation of prosthesis total precarious favored the occurrence of yeasts of the Candida sp, particularly Candida albicans, statistically significant levels
in samples of mucosa and biofilm acceded to total prosthesis, prothetic device, making it a potential reservoir of…
Advisors/Committee Members: Universidade Estadual Paulista (UNESP), Júnior, Elerson Gaetti-Jardim [UNESP], Castro, Alvimar Lima de [UNESP].
Subjects/Keywords: Enterobacterias; Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Marqueti, A. C. [. (2011). Próteses totais removíveis como reservatório de microrganismos oportunistas. (Doctoral Dissertation). Universidade Estadual Paulista (UNESP). Retrieved from http://hdl.handle.net/11449/102338
Chicago Manual of Style (16th Edition):
Marqueti, Antonio Carlos [UNESP]. “Próteses totais removíveis como reservatório de microrganismos oportunistas.” 2011. Doctoral Dissertation, Universidade Estadual Paulista (UNESP). Accessed April 15, 2021.
http://hdl.handle.net/11449/102338.
MLA Handbook (7th Edition):
Marqueti, Antonio Carlos [UNESP]. “Próteses totais removíveis como reservatório de microrganismos oportunistas.” 2011. Web. 15 Apr 2021.
Vancouver:
Marqueti AC[. Próteses totais removíveis como reservatório de microrganismos oportunistas. [Internet] [Doctoral dissertation]. Universidade Estadual Paulista (UNESP); 2011. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/11449/102338.
Council of Science Editors:
Marqueti AC[. Próteses totais removíveis como reservatório de microrganismos oportunistas. [Doctoral Dissertation]. Universidade Estadual Paulista (UNESP); 2011. Available from: http://hdl.handle.net/11449/102338
14.
Amando, Bruno Rocha.
Impregnação de cateteres urinários com moduladores de quorum sensing e inibidor de bombas de efluxo para inibição de biofilmes bacterianos associados a infecção do trato urinário.
Degree: 2018, Brazil
URL: http://www.repositorio.ufc.br/handle/riufc/50226
► Submitted by Carolinda Oliveira ([email protected]) on 2020-02-20T13:36:47Z No. of bitstreams: 1 2018_dis_bramando.pdf: 1839876 bytes, checksum: e8806c56388960b6dd5b68df2316be7a (MD5)
Rejected by Erika Fernandes ([email protected]), reason: Corrigir ficha…
(more)
▼ Submitted by Carolinda Oliveira ([email protected]) on 2020-02-20T13:36:47Z No. of bitstreams: 1 2018_dis_bramando.pdf: 1839876 bytes, checksum: e8806c56388960b6dd5b68df2316be7a (MD5)
Rejected by Erika Fernandes ([email protected]), reason: Corrigir ficha catalográfica on 2020-02-20T13:42:45Z (GMT)
Submitted by Carolinda Oliveira ([email protected]) on 2020-02-20T14:12:32Z No. of bitstreams: 1 2018_dis_bramando.pdf: 1795109 bytes, checksum: d58cce16edac895347844b0da3fc5a8c (MD5)
Approved for entry into archive by Erika Fernandes ([email protected]) on 2020-02-20T14:25:57Z (GMT) No. of bitstreams: 1 2018_dis_bramando.pdf: 1795109 bytes, checksum: d58cce16edac895347844b0da3fc5a8c (MD5)
Made available in DSpace on 2020-02-20T14:25:57Z (GMT). No. of bitstreams: 1 2018_dis_bramando.pdf: 1795109 bytes, checksum: d58cce16edac895347844b0da3fc5a8c (MD5) Previous issue date: 2018-12-17
Urinary tract infection accounts for 40% of
healthcare-related infections and the use of medical devices is an important risk factor for its occurrence. The impregnation with antimicrobial urinary catheters presents good results, but promotes the selective pressure on microorganisms. Thus, the use of quorum sensing modulators and efflux pump inhibitors can be an effective strategy to combat biofilm formation in catheters without causing selective pressure. The objective of this study was to evaluate the in vitro effect of quorum modulators sensing curcumin (CUR) and furaneol (RUF) and the chlorpromazine efflux pump inhibitor (CPZ) impregnated in delay catheters on the formation of biofilms by bacteria associated to urinary tract infection. 18 isolates were used (6 Escherichia coli, 6 Klebsiella pneumoniae and 6 Proteus mirabilis), which were evaluated for sensitivity in the planktonic form, the compounds, by broth microdilution technique. Then the compounds were tested at different concentrations on biofilm formation and on
mature biofilms. Subsequently, the catheters were impregnated with solutions containing CUR (1,024 μM), FUR (10,000 μM) or CPZ (400 μg/ml) and the impregnation was evaluated by scanning electron microscopy (SEM). The evaluation of the formation of biofilms in these catheters was performed by counting CFU and the biofilms were evaluated by confocal microscopy. Finally, the effect of ciprofloxacin and meropenem was evaluated in assays with catheters impregnated or not with CPZ. The CUR and FUR didn’t present inhibitory activity on uropathogens, however, the CPZ had MICs ranging from 39.06 to 625 μg/mL. Cur and CPZ inhibited (P <0.05) in vitro biofilm formation. The dispersive energy system associated with scanning electron microscopy demonstrated the effective impregnation of the catheters with CUR, FUR and CPZ. However, only the CPZ-impregnated catheters inhibited (P <0.05) the formation of biofilms by all species. Confocal microscopy demonstrated robust biofilms on
non-impregnated catheters and a reduction or absence, in some cases, of biofilms on CPZ-impregnated catheters. Finally,…
Advisors/Committee Members: Maia, Débora Castelo Branco de Souza Collares.
Subjects/Keywords: Biofilmes; Enterobacteriaceae; Curcumina; Clorpromazina
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Amando, B. R. (2018). Impregnação de cateteres urinários com moduladores de quorum sensing e inibidor de bombas de efluxo para inibição de biofilmes bacterianos associados a infecção do trato urinário. (Masters Thesis). Brazil. Retrieved from http://www.repositorio.ufc.br/handle/riufc/50226
Chicago Manual of Style (16th Edition):
Amando, Bruno Rocha. “Impregnação de cateteres urinários com moduladores de quorum sensing e inibidor de bombas de efluxo para inibição de biofilmes bacterianos associados a infecção do trato urinário.” 2018. Masters Thesis, Brazil. Accessed April 15, 2021.
http://www.repositorio.ufc.br/handle/riufc/50226.
MLA Handbook (7th Edition):
Amando, Bruno Rocha. “Impregnação de cateteres urinários com moduladores de quorum sensing e inibidor de bombas de efluxo para inibição de biofilmes bacterianos associados a infecção do trato urinário.” 2018. Web. 15 Apr 2021.
Vancouver:
Amando BR. Impregnação de cateteres urinários com moduladores de quorum sensing e inibidor de bombas de efluxo para inibição de biofilmes bacterianos associados a infecção do trato urinário. [Internet] [Masters thesis]. Brazil; 2018. [cited 2021 Apr 15].
Available from: http://www.repositorio.ufc.br/handle/riufc/50226.
Council of Science Editors:
Amando BR. Impregnação de cateteres urinários com moduladores de quorum sensing e inibidor de bombas de efluxo para inibição de biofilmes bacterianos associados a infecção do trato urinário. [Masters Thesis]. Brazil; 2018. Available from: http://www.repositorio.ufc.br/handle/riufc/50226

Montana State University
15.
Aden, David Paul.
In vitro and in vivo studies on transferable drug resistance in the Enterobacteriaceae.
Degree: MS, College of Agriculture, 1970, Montana State University
URL: https://scholarworks.montana.edu/xmlui/handle/1/4697
Subjects/Keywords: Enterobacteriaceae; Antibiotics
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Aden, D. P. (1970). In vitro and in vivo studies on transferable drug resistance in the Enterobacteriaceae. (Masters Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/4697
Chicago Manual of Style (16th Edition):
Aden, David Paul. “In vitro and in vivo studies on transferable drug resistance in the Enterobacteriaceae.” 1970. Masters Thesis, Montana State University. Accessed April 15, 2021.
https://scholarworks.montana.edu/xmlui/handle/1/4697.
MLA Handbook (7th Edition):
Aden, David Paul. “In vitro and in vivo studies on transferable drug resistance in the Enterobacteriaceae.” 1970. Web. 15 Apr 2021.
Vancouver:
Aden DP. In vitro and in vivo studies on transferable drug resistance in the Enterobacteriaceae. [Internet] [Masters thesis]. Montana State University; 1970. [cited 2021 Apr 15].
Available from: https://scholarworks.montana.edu/xmlui/handle/1/4697.
Council of Science Editors:
Aden DP. In vitro and in vivo studies on transferable drug resistance in the Enterobacteriaceae. [Masters Thesis]. Montana State University; 1970. Available from: https://scholarworks.montana.edu/xmlui/handle/1/4697
16.
Luz, Daniela Inocente.
Heterorresistência e resistência adaptativa à Polimixina B em isolados de Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC).
Degree: 2014, Brazil
URL: http://hdl.handle.net/10183/115008
► As enterobactérias constituem importantes agentes causadores de infecções nosocomiais e possuem alta capacidade em adquirir mecanismos de resistência, inclusive aos carbapenêmicos, que são os principais…
(more)
▼ As enterobactérias constituem importantes agentes causadores de infecções nosocomiais e possuem alta capacidade em adquirir mecanismos de resistência, inclusive aos carbapenêmicos, que são os principais fármacos utilizados para o tratamento das infecções causadas por esses microrganismos. Assim, as opções terapêuticas tornaram-se restritas e as polimixinas (polimixina B e colistina) voltaram a ser utilizadas na prática clínica. Estudos descrevem a ocorrência de heterorresistência à colistina em enterobactérias. Para a resistência adaptativa, não há relatos atuais para enterobactérias frente à polimixina B. O objetivo deste trabalho foi avaliar a presença destes dois fenômenos de resistência à polimixina B, e sua estabilidade, em isolados de
Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC), provenientes de pacientes hospitalizados. A avaliação da heterorresistência foi feita através da análise do perfil populacional (PAP)
em 8 isolados de enterobactérias, inoculando-se diluições seriadas dos mesmos em Agar Mueller Hinton contendo diferentes concentrações de polimixina B. Os ensaios de resistência adaptativa foram realizados para os mesmos isolados, submetendo-os ao cultivo em concentrações crescentes de polimixina B. A determinação da estabilidade das subpopulações resistentes foi feita através de passagens dos isolados, por 4 dias consecutivos, em meio livre de antibiótico, e posterior determinação da CIM. A CIM foi reavaliada após 2 e 6 meses de estocagem a -80ºC para os isolados com subpopulações heterorresistentes ou com resistência induzida. Realizou-se técnica de tipagem molecular (PFGE), entre as populações originais e respectivas subpopulações resistentes. Foram avaliados 4 isolados de Klebsiella pneumoniae caracterizadas como 4 clones distintos (K1, K2, K3 e K4), assim como os 3 isolados de Enterobacter cloacae - 2 de clone idêntico (Ec1a e Ec1b), mas com perfis fenotípicos distintos e um
clonalmente distinto (Ec2), e uma cepa de Escherichia coli (E1). As CIM iniciais para polimixina B, realizadas por microdiluição em caldo, foram entre 0,0625 e 0,25 μg/mL. Quatro amostras demonstraram heterorresistência (K1, K2, K3 e K4), as quais cresceram nas concentrações 2 (K2), 3 (K1, K4) e 6 μg/mL (K3), e suas CIM após 4 dias de passagem em meio livre de antibiótico se mantiveram altas (K1 4 μg/mL, K2 e K3 16 μg/mL e K4 2 μg/mL). As subpopulações heterorresistentes representaram 0,000087% a 0,00036% de suas populações originais. Três amostras demonstraram resistência adaptativa (K1, K3 e K4), as quais cresceram até concentração 64 μg/mL de polimixina B, e a CIM após 4 dias de passagem em meio livre de antibiótico foi de 32 μg/mL para os três isolados. As CIM mantiveram-se elevadas após 2 e 6 meses de estocagem, tanto para os isolados heterorresistentes como para aqueles da indução da resistência. Por PFGE, verificou-se a relação clonal entre os isolados clínicos iniciais e as
subpopulações resistentes. Dos 8 isolados estudados, 4 apresentaram heterorresistência e 3 apresentaram resistência…
Advisors/Committee Members: Zavascki, Alexandre Prehn.
Subjects/Keywords: Enterobacteriaceae; Polimixina B; Enterobacteriaceae; Polymyxin B; Heteroresistance; Adaptive resistance
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Luz, D. I. (2014). Heterorresistência e resistência adaptativa à Polimixina B em isolados de Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC). (Masters Thesis). Brazil. Retrieved from http://hdl.handle.net/10183/115008
Chicago Manual of Style (16th Edition):
Luz, Daniela Inocente. “Heterorresistência e resistência adaptativa à Polimixina B em isolados de Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC).” 2014. Masters Thesis, Brazil. Accessed April 15, 2021.
http://hdl.handle.net/10183/115008.
MLA Handbook (7th Edition):
Luz, Daniela Inocente. “Heterorresistência e resistência adaptativa à Polimixina B em isolados de Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC).” 2014. Web. 15 Apr 2021.
Vancouver:
Luz DI. Heterorresistência e resistência adaptativa à Polimixina B em isolados de Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC). [Internet] [Masters thesis]. Brazil; 2014. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/10183/115008.
Council of Science Editors:
Luz DI. Heterorresistência e resistência adaptativa à Polimixina B em isolados de Enterobacteriaceae produtores de Klebsiella pneumoniae carbapenemase (KPC). [Masters Thesis]. Brazil; 2014. Available from: http://hdl.handle.net/10183/115008
17.
Cardoso, Maíra Duarte.
Avaliação comparativa da microbiota de interesse em saúde pública de aves marinhas migratórias e residentes.
Degree: 2018, Brazil
URL: https://www.arca.fiocruz.br/handle/icict/27013
► Made available in DSpace on 2018-06-20T14:16:03Z (GMT). No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) maira_duarte.pdf: 2259230 bytes, checksum: 5c443e9fc21d644d43bd0b55f4a0962e (MD5) Previous issue…
(more)
▼ Made available in DSpace on 2018-06-20T14:16:03Z (GMT). No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) maira_duarte.pdf: 2259230 bytes, checksum: 5c443e9fc21d644d43bd0b55f4a0962e (MD5) Previous issue date: 2018
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Foram pesquisadas e caracterizadas bactérias de interesse em saúde pública em aves marinhas, com ênfase na detecção de diferenças de frequência entre aves migratórias e residentes no litoral brasileiro, uma vez que as aves migratórias podem contaminar o ambiente com espécies microbianas exóticas. Foram coletados, em duplicata, swabs cloacais ou de fezes, e em alguns casos, orais, oculares, traqueais e de lesões, de 122 aves marinhas, sendo 51 não arribadas e residentes; 50 arribadas e residentes; e 21 arribadas e migratórias. Vibrio sp., Aeromonas sp., Salmonella sp. e outras enterobactérias
foram identificadas por métodos bioquímicos. Os perfis antigênicos de Vibrio cholerae e Salmonella sp. foram identificados por soroaglutinação; a caracterização do perfil de susceptibilidade das cepas, por difusão de disco em ágar; a detecção de genes de virulência de Vibrio sp. e Aeromonas sp. e de genes de resistência a quinolonas de Salmonella sp., por reação em cadeia da polimerase (PCR); e a identificação dos perfis de Salmonella sp., por eletroforese em gel de campo pulsado (PFGE). Foram identificadas bactérias de importância em saúde pública, como Vibrio sp. (41%), Aeromonas sp. (21%); e Salmonella sp. (7%). Também foram identificadas outras enterobactérias, sendo Escherichia coli a espécie mais frequente (70%). Foram observadas diferenças entre aves marinhas residentes e migratórias, como o gênero Vibrio e a espécie V. cincinnatiensis, significativamente mais frequentes em aves migratórias. A maioria das cepas multirresistentes a antimicrobianos foi identificada em aves
residentes. Genes de virulência foram detectados em cepas de Aeromonas sp. isoladas a partir de aves residentes e migratórias. Alguns achados importantes se destacaram: a primeira descrição de S. enterica Panama em aves marinhas; a totalidade de cepas de Salmonella resistentes, ou pelo menos intermediárias, aos antimicrobianos testados (com ênfase para a resistência a quinolonas); alta frequência de resistência à cefoxitina em Aeromonas sp.; e a resistência ao imipenem em todas as espécies bacterianas avaliadas. O PFGE sugeriu que os perfis de S. enterica Panama e Newport encontrados ainda não se encontram disseminados no Brasil e que o perfil de S. enterica Typhimurium já esteja disseminado; assim, as aves marinhas podem ser um importante elo da cadeia epidemiológica deste sorovar. O trabalho demonstrou a importância do monitoramento de bactérias em aves marinhas, tanto residentes quanto migratórias, para a detecção precoce de bactérias potencialmente zoonóticas ou que possam ser
transmitidas por alimentos ao homem.
Bacteria of public health interest were…
Advisors/Committee Members: Rodrigues, Dalia dos Prazeres, Siciliano, Salvatore.
Subjects/Keywords: Vibrio; Aeromonas; Aves Marinhas; Salmonella; Enterobacteriaceae; Vibrio; Aeromonas; Salmonella; Enterobacteriaceae; Seabirds; Vibrio; Aeromonas; Salmonella; Aves; Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cardoso, M. D. (2018). Avaliação comparativa da microbiota de interesse em saúde pública de aves marinhas migratórias e residentes. (Doctoral Dissertation). Brazil. Retrieved from https://www.arca.fiocruz.br/handle/icict/27013
Chicago Manual of Style (16th Edition):
Cardoso, Maíra Duarte. “Avaliação comparativa da microbiota de interesse em saúde pública de aves marinhas migratórias e residentes.” 2018. Doctoral Dissertation, Brazil. Accessed April 15, 2021.
https://www.arca.fiocruz.br/handle/icict/27013.
MLA Handbook (7th Edition):
Cardoso, Maíra Duarte. “Avaliação comparativa da microbiota de interesse em saúde pública de aves marinhas migratórias e residentes.” 2018. Web. 15 Apr 2021.
Vancouver:
Cardoso MD. Avaliação comparativa da microbiota de interesse em saúde pública de aves marinhas migratórias e residentes. [Internet] [Doctoral dissertation]. Brazil; 2018. [cited 2021 Apr 15].
Available from: https://www.arca.fiocruz.br/handle/icict/27013.
Council of Science Editors:
Cardoso MD. Avaliação comparativa da microbiota de interesse em saúde pública de aves marinhas migratórias e residentes. [Doctoral Dissertation]. Brazil; 2018. Available from: https://www.arca.fiocruz.br/handle/icict/27013
18.
Faria, Helena Maria Ribeiro.
Controlo da qualidade microbiológica em carcaças de bovinos e suínos, e implementação de medidas preventivas.
Degree: 2016, RCAAP
URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/5721
► Dissertação de Mestrado em Biotecnologia e Qualidade Alimentar
Um quinto da população mundial alimenta-se de carne. Portanto, é necessário controlar as indústrias que levam este…
(more)
▼ Dissertação de Mestrado em Biotecnologia e Qualidade Alimentar
Um quinto da população mundial alimenta-se de carne. Portanto, é necessário controlar as indústrias que levam este alimento até ao prato do consumidor. Os critérios microbiológicos dão orientações relativamente à aceitação dos géneros alimentares e seus processos de fabrico, manipulação e distribuição. Os operadores das empresas do setor alimentar devem assegurar que os alimentos cumprem os critérios microbiológicos estabelecidos no anexo I do Regulamento (CE) nº 1441/2007. O objetivo deste trabalho é a avaliação da qualidade microbiológica em carcaças de bovinos e suínos após o abate, mas antes da refrigeração, recorrendo-se para isso à quantificação de Microrganismos a 30ºC, Enterobacteriaceae e pesquisa de Salmonella.
O estudo começou pela recolha das amostras em dois matadouros localizados na zona Norte de Portugal, durante um período entre novembro de 2012 e abril de 2013. Foram efetuadas um total de 150 colheitas, das quais, para a Empresa A, 50 de bovino e 50 de suíno, e para a Empresa B , 50 de suíno. A escolha dos pontos de amostragem e o método utilizado foram regidos de acordo com a norma ISO 17604 “Microbiology of food and animal feeding stuffs - carcass sampling for microbiological analysis”. Foram amostradas cinco carcaças de cada espécie animal, por cada colheita e o método utilizado foi o não-destrutivo. Nas colheitas, para Microrganismos a 30ºC e Enterobacteriaceae, foram amostrados quatro pontos de cada carcaça, e para a pesquisa de Salmonella, foi amostrado um ponto.
O tratamento das amostras foi efetuado na empresa Equilibrium, Laboratório de Controlo da Qualidade e de Processos, Lda., acreditado no âmbito da ISO EN/IEC 17025 para águas e alimentos. Para quantificação de Microrganismos a 30ºC, usou-se a técnica de incorporação em gelose, com o meio PCA (ISO 4833), e para quantificação de Enterobacteriaceae usou-se o meio VRBG (ISO 21528). A pesquisa de Salmonella decorreu segundo os procedimentos descritos na norma ISO 6579.
Os resultados obtidos permitem observar que, quer para a Empresa A, quer para a Empresa B, em ambas as espécies animais, a maior parte das amostras apresentaram índices de contaminação abaixo do limite mínimo estipulado no Regulamento (CE) nº 1441/2007. Na Empresa A, as carcaças de bovinos apresentaram uma média logarítmica de 3,09 log ufc/cm2 na análise de microrganismos a 30ºC e 0,32 log ufc/cm2 na análise de Enterobacteriaceae, quanto às carcaças de suínos, para microrganismos a 30ºC obteve-se uma média de 2,57 log ufc/cm2 e para Enterobacteriaceae, 0,11 log ufc/cm2. No caso da Empresa B obteve-se uma média logarítmica de 2,89 log ufc/cm2 para microrganismos a 30ºC e 0,74 log ufc/cm2 para Enterobacteriaceae. Em relação à pesquisa de Salmonella, não se verificaram resultados positivos em nenhuma carcaça analisada.
Sendo a carne um meio de cultura favorável ao crescimento microbiano, é relevante salientar que todas as medidas de higiene são importantes para evitar contaminações desta.
Estes valores refletem que…
Advisors/Committee Members: Saavedra, Maria José Félix, Antão, Maria Cristina de Morais Caldas.
Subjects/Keywords: Microbiologia; Qualidade; Bovinos; Suínos; Enterobacteriaceae; Salmonella
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Faria, H. M. R. (2016). Controlo da qualidade microbiológica em carcaças de bovinos e suínos, e implementação de medidas preventivas. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/5721
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Faria, Helena Maria Ribeiro. “Controlo da qualidade microbiológica em carcaças de bovinos e suínos, e implementação de medidas preventivas.” 2016. Thesis, RCAAP. Accessed April 15, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/5721.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Faria, Helena Maria Ribeiro. “Controlo da qualidade microbiológica em carcaças de bovinos e suínos, e implementação de medidas preventivas.” 2016. Web. 15 Apr 2021.
Vancouver:
Faria HMR. Controlo da qualidade microbiológica em carcaças de bovinos e suínos, e implementação de medidas preventivas. [Internet] [Thesis]. RCAAP; 2016. [cited 2021 Apr 15].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/5721.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Faria HMR. Controlo da qualidade microbiológica em carcaças de bovinos e suínos, e implementação de medidas preventivas. [Thesis]. RCAAP; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/5721
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
19.
Costa, Rui André Dias.
Ocorrência de Enterobacteriaceae, E. coli e E. coli O157 em pele e em carcaças de suínos abatidos em matadouro.
Degree: 2016, RCAAP
URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6191
► Dissertação de Mestrado em Segurança Alimentar
Neste estudo efetuou-se a determinação da contagem de Enterobacteriaceae e E. coli e a ocorrência de E. coli O157…
(more)
▼ Dissertação de Mestrado em Segurança Alimentar
Neste estudo efetuou-se a determinação da contagem de Enterobacteriaceae e E. coli e a ocorrência de E. coli O157 na pele de suíno e nas faces internas e externas das carcaças de 120 suínos abatidos num Matadouro de Suínos da Região Norte de Portugal. Pretendeu-se observar se o processo de abate reduz o nível das bactérias supracitadas; se os procedimentos efetuados pelos operadores do Matadouro estão a ser eficazes após a evisceração bem como a possível relação entre os níveis de Enterobacteriaceae e E. coli. O método de amostragem utilizado foi o esfregaço com esponja de área 1000 cm2, segundo o protocolo preconizado pela Food Standards Agency. Efetuou-se adicionalmente a contagem de Enterobacteriaceae na face externa da carcaça de 51 suínos utilizando a metodologia de amostragem definida no Regulamento (CE) N.º 1441/2007 (excisão), com o objetivo de comparar qual a técnica de amostragem mais eficaz. Nesta investigação também se determinou a influência de alguns potenciais fatores de risco (nível de sujidade fecal, tempo de permanência na abegoaria e momento de abate) do nível de Enterobacteriaceae e E. coli e na presença de E. coli O157.
Após análise dos resultados verificou-se uma diferença altamente significativa entre o nível médio de Enterobacteriaceae e E. coli na pele de suíno e o encontrado na face externa (p-value <0,001), assim como entre o nível médio de Enterobacteriaceae e E. coli na face interna da carcaça comparativamente à face externa (p-value <0,001) e que existe uma relação altamente significativa entre os níveis de Enterobacteriaceae e E. coli (p-value <0,001) nas amostras testadas. Neste estudo não foram detetadas colónias E. coli O157 nem outras VTEC.
Também se constatou que o método de excisão revelou valores de Enterobacteriaceae superiores comparativamente ao método do esfregaço com esponja, tendo sido esta diferença altamente significativa (p-value <0,001).
Por último foi possível concluir que entre o nível de Enterobacteriaceae e E. coli na pele de suíno e na face externa e entre o tempo de permanência na abegoaria existe uma relação altamente significativa (p-value <0,001) e que não houve relação significativa com o nível de sujidade (p-value >0,05). Quanto ao momento de abate, os suínos abatidos no início apresentaram maiores níveis de Enterobacteriaceae e de E. coli na pele de suíno e face externa da carcaça, sendo as diferenças significativas (p-value <0,05) e altamente significativas (p-value <0,001), assim respetivamente.
The goal of this study was to determine the number of Enterobacteriaceae and E. coli and the occorrence of E. coli O157 in pig skin. This was done for the internal and external carcasses of 120 pigs slaughtered in a slaughterhouse at the Northen Region of Portugal. It was intended to be observe if the slaughter process reduces the level of these bacteria, if the procedures performed by operators of the slaughterhouse are being effective after evisceration and if there is a relation between the levels of…
Advisors/Committee Members: Pinto, Madalena Vieira.
Subjects/Keywords: Enterobacteriaceae; Escherichia coli; Suíno; Pele; Carcaça; Contaminação
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Costa, R. A. D. (2016). Ocorrência de Enterobacteriaceae, E. coli e E. coli O157 em pele e em carcaças de suínos abatidos em matadouro. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6191
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Costa, Rui André Dias. “Ocorrência de Enterobacteriaceae, E. coli e E. coli O157 em pele e em carcaças de suínos abatidos em matadouro.” 2016. Thesis, RCAAP. Accessed April 15, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6191.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Costa, Rui André Dias. “Ocorrência de Enterobacteriaceae, E. coli e E. coli O157 em pele e em carcaças de suínos abatidos em matadouro.” 2016. Web. 15 Apr 2021.
Vancouver:
Costa RAD. Ocorrência de Enterobacteriaceae, E. coli e E. coli O157 em pele e em carcaças de suínos abatidos em matadouro. [Internet] [Thesis]. RCAAP; 2016. [cited 2021 Apr 15].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6191.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Costa RAD. Ocorrência de Enterobacteriaceae, E. coli e E. coli O157 em pele e em carcaças de suínos abatidos em matadouro. [Thesis]. RCAAP; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6191
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Silva, Vanessa Iolanda Fonseca Nascimento Ferreira da.
Ocorrência de Salmonella spp. e Enterobacteriaceae em carcaças e pele de suínos abatidos em matadouro.
Degree: 2016, RCAAP
URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6192
► Dissertação de Mestrado em Segurança Alimentar
Salmonella spp. é dos principais agentes patogénicos que causam toxinfeções alimentares. Em Portugal, entre 2009 e 2013, observou-se que…
(more)
▼ Dissertação de Mestrado em Segurança Alimentar
Salmonella spp. é dos principais agentes patogénicos que causam toxinfeções alimentares. Em Portugal, entre 2009 e 2013, observou-se que 12% das toxinfeções alimentares foram causadas por este agente, dos quais S. Enteritidis e S. Typhimurium foram os serótipos mais identificados.
Logo após à carne de aves, a carne de suíno surge como o principal veículo de transmissão, que origina salmonelose.
O principal propósito deste estudo foi verificar a ocorrência de Salmonella spp. e de Enterobacteriaceae em carcaças e em pele de suíno abatidos em matadouro e avaliar a influência de alguns fatores de risco na sua ocorrência: o tempo de permanência na abegoaria, o momento de abate (início/fim) e a sujidade fecal visível na pele do suíno antes do abate.
As amostras foram recolhidas em duas etapas diferentes da linha de abate: após a sangria efetuou-se uma zaragatoa com esponja na pele do suíno e, antes da refrigeração efetuou-se uma zaragatoa com esponja na face externa e interna da respetiva carcaça.
Neste estudo verificou-se que no total das amostras analisadas (56/360), 15,6% revelaram a presença de Salmonella spp., das quais 29,2% (35/120) foram isoladas da pele do suíno, 13,3% na face interna da carcaça (16/120) e 4,2% na face externa (5/120). Relativamente ao nível de Enterobacteriaceae onde? verificamos uma média de 1,491 ± 1,559 (log10 ufc/cm2) valores. O teste de t-student revelou existir uma diferença significativa entre este parâmetro e a presença de Salmonella spp. na pele do suíno (p-value = 0,030) e na face interna da carcaça (p-value = 0,0015).
Após a análise estatística dos dados, verificou-se que os suínos com um maior tempo de permanência na abegoaria revelaram um maior nível de Enterobacteriaceae na sua pele e na face externa das carcaças, sendo esta diferença altamente significativa (p-value < 0,001).
Ainda, os resultados estatísticos não revelam diferenças significativas no isolamento da Salmonella spp. na pele do suíno com o tempo de permanência na abegoaria (p-value > 0,05), contudo os suínos sem Salmonella spp. tiveram em média um tempo de permanência na abegoaria inferior aos dos suínos que evidenciam Salmonella spp.
Também se constatou, que apesar das diferenças não terem sido estatisticamente significativas, o isolamento de Salmonella spp. na superfície da pele dos suínos foi superior nos animais abatidos no início comparativamente aos animais abatidos na fase final. O nível
de Enterobacteriaceae na pele do suíno também revelou ser maior nos suínos abatidos no início (p-value < 0,05).
Finalmente, o nível visual de sujidade da pele do suíno não revelou ser um bom indicador de presença de Salmonella spp. (p-value = 0,19) e do nível de Enterobacteriaceae (p-value > 0,05) na pele do suíno, não devendo ser considerado como um indicador de contaminação por estas bactérias.
Salmonella spp. is one of the major pathogens which cause food poisoning. In Portugal, between 2009 and 2013, it was verified that 12% of food poisoning cases were caused by…
Advisors/Committee Members: Pinto, Madalena Vieira.
Subjects/Keywords: Salmonella; Enterobacteriaceae; Suíno; Pele; Carcaça; Contaminação
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Silva, V. I. F. N. F. d. (2016). Ocorrência de Salmonella spp. e Enterobacteriaceae em carcaças e pele de suínos abatidos em matadouro. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6192
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Silva, Vanessa Iolanda Fonseca Nascimento Ferreira da. “Ocorrência de Salmonella spp. e Enterobacteriaceae em carcaças e pele de suínos abatidos em matadouro.” 2016. Thesis, RCAAP. Accessed April 15, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6192.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Silva, Vanessa Iolanda Fonseca Nascimento Ferreira da. “Ocorrência de Salmonella spp. e Enterobacteriaceae em carcaças e pele de suínos abatidos em matadouro.” 2016. Web. 15 Apr 2021.
Vancouver:
Silva VIFNFd. Ocorrência de Salmonella spp. e Enterobacteriaceae em carcaças e pele de suínos abatidos em matadouro. [Internet] [Thesis]. RCAAP; 2016. [cited 2021 Apr 15].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6192.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Silva VIFNFd. Ocorrência de Salmonella spp. e Enterobacteriaceae em carcaças e pele de suínos abatidos em matadouro. [Thesis]. RCAAP; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/6192
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong
21.
Choy, Wai-kit.
Detection and
characterization of extended-spectrum beta-lactamases among blood
isolates of Providencia stuartii in Hong Kong.
Degree: 2005, University of Hong Kong
URL: http://hdl.handle.net/10722/131546
Subjects/Keywords: Beta
lactamases.;
Enterobacteriaceae.
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Choy, W. (2005). Detection and
characterization of extended-spectrum beta-lactamases among blood
isolates of Providencia stuartii in Hong Kong. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/131546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Choy, Wai-kit. “Detection and
characterization of extended-spectrum beta-lactamases among blood
isolates of Providencia stuartii in Hong Kong.” 2005. Thesis, University of Hong Kong. Accessed April 15, 2021.
http://hdl.handle.net/10722/131546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Choy, Wai-kit. “Detection and
characterization of extended-spectrum beta-lactamases among blood
isolates of Providencia stuartii in Hong Kong.” 2005. Web. 15 Apr 2021.
Vancouver:
Choy W. Detection and
characterization of extended-spectrum beta-lactamases among blood
isolates of Providencia stuartii in Hong Kong. [Internet] [Thesis]. University of Hong Kong; 2005. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/10722/131546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Choy W. Detection and
characterization of extended-spectrum beta-lactamases among blood
isolates of Providencia stuartii in Hong Kong. [Thesis]. University of Hong Kong; 2005. Available from: http://hdl.handle.net/10722/131546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Nelson Mandela Metropolitan University
22.
Gqunta, Kwanele.
Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth.
Degree: Faculty of Science, 2014, Nelson Mandela Metropolitan University
URL: http://hdl.handle.net/10948/d1020944
► Multidrug resistant (MDR) extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Gram-negative bacteria have become an international health issue limiting treatment options. The objective of this study was…
(more)
▼ Multidrug resistant (MDR) extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Gram-negative bacteria have become an international health issue limiting treatment options. The objective of this study was to determine the prevalence of carbapenem-susceptible (CS) and carbapenem-resistant (CR) Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa and investigate clinical isolates for their resistant genes/ determinants. A total of 98 bacterial isolates (59 CS and 39 CR) were collected between February 2012 and February 2013 at NHLS, after being recovered from various clinical specimens from PE hospital complex. Antimicrobial susceptibility testing was performed using the VITEK 2® system, E-test and microbroth dilution method. PCR and DNA sequencing were used to investigate: (i) ESBLs: CTX-M, TEM, SHV and OXA-1; (ii) plasmidmediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrC, qnrD, qnrS, qepA and aac(6’)-lb-cr; (iii) Escherichia coli sequence type 131 (ST131); (iv) carbapenemases: NDM, VIM, IMP, KPC, BIC, SME, IMI, NMC-A, GES, OXA-23, OXA-24, OXA-48, OXA-51 and OXA-58; and (v) insertion sequence ISAba1 upstream blaOXA-23/-24/-51/-58 genes. Porin loss in CR isolates was determined by SDSPAGE while genetic relatedness between E. coli ST131 isolates was determined by pulsed-field gel electrophoresis (PFGE). MDR ESBL-producing Enterobacteriaceae (mainly K. pneumoniae) and CR A. baumannii isolates were recovered from neonatal/ infant specimens. The majority of CS and CR isolates were MDR, possessing multiple ESBL genotypes (CTX-M, TEM, SHV and OXA-1). ESBL variants identified included: CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-22, CTX-M-9, CTX-M-14, TEM-1, SHV-1, SHV-11 and OXA-1. PMQRs identified included: aac(6’)- lb-cr, qnrB1, qnrB2, qnrB13 and qnrS1. Twelve of 21 (57.1 percent) E. coli isolates belonged to the ST131 clonal complex and were genetically diverse, mainly producing CTX-M-15. Carbapenem resistance mechanisms identified included: (i) OXA-23 preceded by ISAba1 in 10 A. baumannii and 2 P. aeruginosa isolates; (ii) IMI-2 carbapenemase in an E. asburiae isolate; and (iii) combination of porin loss and ESBL production in 1 K. pneumoniae and 1 E. coli isolate. This is the first report in South Africa describing the occurrence of CTX-M-9, CTX-M-22 and IMI-2 among Enterobacteriaceae; CTX-M-15 in A. baumannii; and OXA-23 in combination with OXA-51 in P. aeruginosa. However, resistance determinants could not be detected for 24 carbapenem-resistant isolates which requires further investigation.
Subjects/Keywords: Beta lactamases; Enterobacteriaceae – South Africa – Port Elizabeth
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gqunta, K. (2014). Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/d1020944
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gqunta, Kwanele. “Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth.” 2014. Thesis, Nelson Mandela Metropolitan University. Accessed April 15, 2021.
http://hdl.handle.net/10948/d1020944.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gqunta, Kwanele. “Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth.” 2014. Web. 15 Apr 2021.
Vancouver:
Gqunta K. Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2014. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/10948/d1020944.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gqunta K. Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth. [Thesis]. Nelson Mandela Metropolitan University; 2014. Available from: http://hdl.handle.net/10948/d1020944
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University
23.
Hackland, Peter Linton.
Structural analysis of some Escherichia coli capsular antigens.
Degree: Faculty of Pharmacy, Pharmacy, 1992, Rhodes University
URL: http://hdl.handle.net/10962/d1003236
► The work presented in this thesis forms part of a collaborative effort to determine the chemical structures of the surface antigens of bacteria which belong…
(more)
▼ The work presented in this thesis forms part of a collaborative effort to determine the chemical structures of the surface antigens of bacteria which belong to the Enterobacteriaceae. These antigens are largely polysaccharides and occur as lipopolysaccharides and capsular polysaccharides which give rise to the somatic or 0 antigens and the capsular or K antigens, respectively. In recent years interest has mostly been focused on the extracellular polysaccharide antigens expressed by the genus Escherichia coli because of the effect they exert on normal immunological processes and their structural relatedness to the surface antigens of other more pathogenic bacteria. Therefore the molecular structures of the capsular polysaccharides (Kantigens)produced by E. coli 09:K35(AI04a) and 09:K38(A262a) have been determined by novel enzymic, chemical and spectroscopic procedures. These investigations show that the structures of these polysaccharides can be determined by a combination of chemical and spectroscopic procedures , or almost entirely by n.m.r. spectroscopy alone. The in vitro bacteriophage mediated depolymerisation of the native E. coli K35 polysaccharide demonstrates the value of this method for the isolation of oligosaccharides representing the repeating- unit and multiples thereof. Finally E. coli K37 and K38 capsular polysaccharides were used as model compounds for the evaluation of partial and selective reductive cleavage as methods of generating oligosaccharide for further structural analysis. The products of these reactions were analysed largely by a combination of mass spectrometric procedures.
Subjects/Keywords: Antigens; Enterobacteriaceae; Escherichia
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hackland, P. L. (1992). Structural analysis of some Escherichia coli capsular antigens. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1003236
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hackland, Peter Linton. “Structural analysis of some Escherichia coli capsular antigens.” 1992. Thesis, Rhodes University. Accessed April 15, 2021.
http://hdl.handle.net/10962/d1003236.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hackland, Peter Linton. “Structural analysis of some Escherichia coli capsular antigens.” 1992. Web. 15 Apr 2021.
Vancouver:
Hackland PL. Structural analysis of some Escherichia coli capsular antigens. [Internet] [Thesis]. Rhodes University; 1992. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/10962/d1003236.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hackland PL. Structural analysis of some Escherichia coli capsular antigens. [Thesis]. Rhodes University; 1992. Available from: http://hdl.handle.net/10962/d1003236
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science
24.
Sonowal, Robert.
Investigations on the Possible Role of Aromatic β-Glucoside Metabolism in Self-Defense in Enterobacteriaceae.
Degree: PhD, Faculty of Science, 2018, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/3312
► Bacteria are ubiquitous in all ecosystems and are often challenged by multiple stresses such as extreme temperatures, high salt concentrations, nutrient limitation, pH variations, radiation,…
(more)
▼ Bacteria are ubiquitous in all ecosystems and are often challenged by multiple stresses such as extreme temperatures, high salt concentrations, nutrient limitation, pH variations, radiation, predation and the presence of antibiotics/toxins. The most challenging among them is predation pressure which is one of the major causes of their mortality in different niches. Bacteria have evolved different adaptive measures to counter predation. Some of them include change in shape, size, motility, and unpalatable aggregate formation.
Aromatic β-glucosides such as salicin, produced by plants as secondary metabolites, play a significant role in protecting them from herbivores. Members of the family Enterobaceriaceae primarily present in soil, e.g. Erwinia chrysanthemi (a phytopathogen) and Klebsiella aerogenes, can utilize the aromatic β-glucosides salicin and arbutin (likely to be present in soil derived from decomposing plant materials) as a carbon source unlike their fellow members such as Escherichia coli, Shigella sonnei, and Salmonella present in the gut environment. Bacteria can obtain energy by metabolizing β-glucosides in the form of glucose. Whether they can also use these molecules as defense tools in a manner similar to plants is an intriguing possibility. In such an event, Bgl+ bacteria could derive a dual advantage in terms of energy generation and protection from predation. The current study was initiated to investigate a possible link between β-glucoside metabolism and self-defense in
Enterobacteriaceae. Different members of
Enterobacteriaceae comprising of both laboratory strains and natural isolates were considered as prey. Predators included were laboratory strains and soil isolates of bacteriovorous nematodes of the Rhabditidae family, the amoeba Dictyostelium discoidium and a bacteriovorous Streptomyces sp. The predator-prey interaction was analyzed by performing viability and behavioral assays in the context of β-glucoside metabolism
Results presented in Chapter 2 show that active catabolism of aromatic β¬glucosides like salicin, arbutin and esculin by Bgl+ bacteria decreases the viability of their predators. The aglycone products released during β-glucosides metabolism, e.g. saligenin in the case of salicin, are the causative agents of the mortality of the predators. The lethality is reversible up to a specific threshold of exposure. Saligenin acts as a chemo-attractant that lures and kills Caenorhabditis elegans N2. In the case of nematodes that succumb, bacteria can derive nutrition from the dead predators indicating a conversion of prey to predator. Experiments with mutant strains of Caenorhabditis elegans suggest that the dopaminergic receptor dop-1 is involved in mediating saligenin toxicity.
Studies mentioned in Chapter 3 revolve around the relevance of the predator-prey interaction discussed in Chapter 2 in the natural environment. Members of
Enterobacteriaceae and their predator amoebae (cellular slime molds) and nematodes were isolated from soil. They show coexistence in most of the soil…
Advisors/Committee Members: Mahadevan, S (advisor).
Subjects/Keywords: Enterobacteriaceae; Beta-Glucoside Metabolism - Enterobacteriaceae; Predator-Prey Interactions - Enterobacteriaceae; Aromatic Beta Glucoside Metabolism; Bacteria - Ecology; Predator Prey Dynamics - Enterobacteriaceae; Bacterial Genetics; β-glucosides; Shigella sonnei; Bacteriology
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sonowal, R. (2018). Investigations on the Possible Role of Aromatic β-Glucoside Metabolism in Self-Defense in Enterobacteriaceae. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3312
Chicago Manual of Style (16th Edition):
Sonowal, Robert. “Investigations on the Possible Role of Aromatic β-Glucoside Metabolism in Self-Defense in Enterobacteriaceae.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed April 15, 2021.
http://etd.iisc.ac.in/handle/2005/3312.
MLA Handbook (7th Edition):
Sonowal, Robert. “Investigations on the Possible Role of Aromatic β-Glucoside Metabolism in Self-Defense in Enterobacteriaceae.” 2018. Web. 15 Apr 2021.
Vancouver:
Sonowal R. Investigations on the Possible Role of Aromatic β-Glucoside Metabolism in Self-Defense in Enterobacteriaceae. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Apr 15].
Available from: http://etd.iisc.ac.in/handle/2005/3312.
Council of Science Editors:
Sonowal R. Investigations on the Possible Role of Aromatic β-Glucoside Metabolism in Self-Defense in Enterobacteriaceae. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3312

University of KwaZulu-Natal
25.
Founou, Luria Leslie.
Molecular epidemiology of livestock-associated Methicillin resistant Staphylococcus aureus (LA-MRSA) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in pigs and exposed workers in Cameroon and South Africa.
Degree: 2017, University of KwaZulu-Natal
URL: https://researchspace.ukzn.ac.za/handle/10413/17557
► Livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) and extendedspectrum β-Lactamase (ESBL)-producing Enterobacteriaceae (ESBL-PE) are amongst the main resistant bacteria of serious public health concern worldwide. Between…
(more)
▼ Livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) and extendedspectrum β-Lactamase (ESBL)-producing
Enterobacteriaceae (ESBL-PE) are amongst the
main resistant bacteria of serious public health concern worldwide. Between March and
October 2016, MRSA and ESBL-PE were identified from 288 nasal and rectal pooled samples
collected from 432 pigs as well as from nasal and hand swabs sampled from 82 humans in five
abattoirs in both Cameroon and South Africa. Carriage, risk factors, antibiotic resistance
profiles, genotypic characteristics and clonal relatedness of circulating MRSA and ESBL-PE
in pigs and humans were investigated using various microbiological (selective agar,
biochemical testing, VITEK 2 system) and molecular methods (REP-PCR, ERIC-PCR and
whole genome sequencing) and compared with statistical tools.
Of the 288 pooled samples, methicillin resistant staphylococci (MRS) were isolated from
108/144 (75%) and 102/144 (70%) of the pooled nasal and rectal samples, respectively. Only
one (0.07%) and four (16.66%) MRSA were detected from pigs in Cameroon and South Africa,
respectively. None of the workers were colonized by MRSA. Genome analysis revealed that
all circulating MRSA isolates belonged to the clonal lineage ST398.
ESBL-PE were isolated from 75% (108/144) and 71% (102/144) of the pooled nasal and rectal
samples, respectively. ESBL-PE prevalence in animal samples from Cameroon was higher than
for South Africa whereas human ESBL-PE carriage was observed in Cameroonian workers
only. Some E. coli and K. pneumoniae isolates detected in humans were highly related to those
isolated from pigs within and across countries. The circulating K. pneumoniae strains were
dominated by the sequence types (ST) 14 and 39. The “high-risk” ST307 clone and two novel
STs assigned ST2958 and ST2959 were also detected.
LA-MRSA ST398 is already present in both Cameroon and South Africa and is probably
underestimated in the absence of molecular epidemiological studies. The high prevalence of
ESBL-PE in pigs in both countries as well as in humans in Cameroon highlights their active
dissemination in the farm-to-plate continuum. LA-MRSA and ESBL-PE are serious food safety
and public health threats requiring the urgent implementation of multi-sectorial, multi-faceted,
and, sustainable interventions among all stakeholders involved in this continuum to contain
their emergence and zoonotic transmission.
Advisors/Committee Members: Essack, Sabiha Yusuf. (advisor), Djoko, Cyrille Le Duc Finyom. (advisor).
Subjects/Keywords: Staphylococcus aureus.; β-Lactamase.; Enterobacteriaceae.; Resistant bacteria.
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Founou, L. L. (2017). Molecular epidemiology of livestock-associated Methicillin resistant Staphylococcus aureus (LA-MRSA) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in pigs and exposed workers in Cameroon and South Africa. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/17557
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Founou, Luria Leslie. “Molecular epidemiology of livestock-associated Methicillin resistant Staphylococcus aureus (LA-MRSA) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in pigs and exposed workers in Cameroon and South Africa.” 2017. Thesis, University of KwaZulu-Natal. Accessed April 15, 2021.
https://researchspace.ukzn.ac.za/handle/10413/17557.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Founou, Luria Leslie. “Molecular epidemiology of livestock-associated Methicillin resistant Staphylococcus aureus (LA-MRSA) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in pigs and exposed workers in Cameroon and South Africa.” 2017. Web. 15 Apr 2021.
Vancouver:
Founou LL. Molecular epidemiology of livestock-associated Methicillin resistant Staphylococcus aureus (LA-MRSA) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in pigs and exposed workers in Cameroon and South Africa. [Internet] [Thesis]. University of KwaZulu-Natal; 2017. [cited 2021 Apr 15].
Available from: https://researchspace.ukzn.ac.za/handle/10413/17557.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Founou LL. Molecular epidemiology of livestock-associated Methicillin resistant Staphylococcus aureus (LA-MRSA) and extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in pigs and exposed workers in Cameroon and South Africa. [Thesis]. University of KwaZulu-Natal; 2017. Available from: https://researchspace.ukzn.ac.za/handle/10413/17557
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal
26.
Estaleva, Calvina Ernesto Langa.
Extended spectrum B-lactamase and plasmid mediated AmpC resistance in clinical isolates of escherichia coli from the central hospital of Maputo, Mozambique.
Degree: 2016, University of KwaZulu-Natal
URL: https://researchspace.ukzn.ac.za/handle/10413/17698
► Antibiotic resistance is one of the main public health problems worldwide, reducing treatment options and increasing morbidity and mortality. The production of extendedspectrum β-lactamases (ESBLs),…
(more)
▼ Antibiotic resistance is one of the main public health problems worldwide, reducing
treatment options and increasing morbidity and mortality. The production of extendedspectrum β-lactamases (ESBLs), plasmid mediated (pAmpC) β-lactamases are the most
important resistance mechanisms that hamper antimicrobial treatment of infections
caused by
Enterobacteriaceae. This study describes the detection and characterization of
pAmpC- and/or ESBL-producing clinical isolates of Escherichia coli (n=230) from
urine and blood samples at the Central Hospital of Maputo, Mozambique from midAugust to mid-November 2015. Antimicrobial susceptibility testing was performed by
the disc diffusion method. Isolates with reduced susceptibility to cefotaxime and/ or
ceftazidime (n=75) were subjected to phenotypic AmpC- and/or ESBL testing as well as
PCR-detection of blaCTX-M, blaTEM, blaSHV, blaCMY, blaMOX, blaFOX and blaDHA. A total
of 75/230 (32.6 %) isolates were ESBL positive, and twenty-five of these were pAmpC
positive. The most prevalent ESBL and pAmpC were CTX-M (77%) and FOX (32%),
respectively. Most CTX-M negative ESBL-strains were blaSHV positive indicating a
SHV-ESBL-type. The presence of co-resistance (R and I) to clinically important
antibiotics were also frequent; blood ciprofloxacin (CIP; n= 12/17: 70.6%), gentamicin
(GEN; n= 8/17: 47.1 %) and trimethoprim-sulfamethoxazole (SXT; n= 17/17; 100%)
and urine CIP (n=40/58; 68.9%), GEN (n= 27/58; 46.5 %) and SXT (n= 55/58; 94.8%).
Multidrug resistance was observed in 17/17 (100 %) and 58/58 (100 %) blood and
urinary isolates, respectively. ERIC-PCR analysis revealed a large genetic diversity of
strains with some minor clusters indicating intra hospital spread.
The study has shown that: (i) a large proportion of clinical isolates of E. coli from the
urinary tract and blood cultures from the Central Hospital are pAmpC and/or ESBLproducing. (ii) CTX-Ms and FOX were the dominant ESBL- and pAmpC-types,
respectively. (iii) All ESBL- and pAmpC-producing strains were MDR-strains only
susceptible to antibiotics that are not easily available in the current location. The overall
findings strongly support the urgent need for strengthened and rapid diagnostic services
to guide correct treatment of serious life-threatening infections and improved infection
control measurements.
Advisors/Committee Members: Sundsfjord, Arnfinn Staale. (advisor), Essack, Sabiha Yusuf. (advisor), Zimba, Tomas Francisco. (advisor).
Subjects/Keywords: β-lactamases.; Antibiotic.; Enterobacteriaceae.; Escherichia coli.; Mozambique.
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Estaleva, C. E. L. (2016). Extended spectrum B-lactamase and plasmid mediated AmpC resistance in clinical isolates of escherichia coli from the central hospital of Maputo, Mozambique. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/17698
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Estaleva, Calvina Ernesto Langa. “Extended spectrum B-lactamase and plasmid mediated AmpC resistance in clinical isolates of escherichia coli from the central hospital of Maputo, Mozambique.” 2016. Thesis, University of KwaZulu-Natal. Accessed April 15, 2021.
https://researchspace.ukzn.ac.za/handle/10413/17698.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Estaleva, Calvina Ernesto Langa. “Extended spectrum B-lactamase and plasmid mediated AmpC resistance in clinical isolates of escherichia coli from the central hospital of Maputo, Mozambique.” 2016. Web. 15 Apr 2021.
Vancouver:
Estaleva CEL. Extended spectrum B-lactamase and plasmid mediated AmpC resistance in clinical isolates of escherichia coli from the central hospital of Maputo, Mozambique. [Internet] [Thesis]. University of KwaZulu-Natal; 2016. [cited 2021 Apr 15].
Available from: https://researchspace.ukzn.ac.za/handle/10413/17698.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Estaleva CEL. Extended spectrum B-lactamase and plasmid mediated AmpC resistance in clinical isolates of escherichia coli from the central hospital of Maputo, Mozambique. [Thesis]. University of KwaZulu-Natal; 2016. Available from: https://researchspace.ukzn.ac.za/handle/10413/17698
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Beyrouthy, Racha.
Entérobactéries résistantes aux carbapénèmes isolées au Nord Liban : mécanismes, support génétique et pathogénicité : Enterobacteriaceae resistant to carbapenems isolated in North Lebanon : mechanisms, genetic support and pathogenicity.
Degree: Docteur es, Sciences de la vie et de la sante, 2014, Clermont-Ferrand 1
URL: http://www.theses.fr/2014CLF1MM10
► Les carbapénèmes sont des antibiotiques de la famille des β-lactamines utilisées en dernier recours à cause de leur stabilité vis-à-vis de la plupart des mécanismes…
(more)
▼ Les carbapénèmes sont des antibiotiques de la famille des β-lactamines utilisées en dernier recours à cause de leur stabilité vis-à-vis de la plupart des mécanismes de résistance. Cependant, on assiste chez les entérobactéries à l’émergence de carbapénèmases capables d’inactiver ces molécules. Les objectives de ce travail étaient d’explorer l’émergence de ces mécanismes de résistance dans des entérobactéries isolées au Nord Liban entre 2008 et 2012, d’analyser leur support génétique, ainsi que le fond génétique et la pathogénicité des souches porteuses. Nous avons observé une augmentation d’un facteur quatre de la prévalence des entérobactéries de sensibilité diminuée ou résistantes aux carbapénèmes dans les prélèvements cliniques hospitaliers entre 2008 et 2012. Un portage intestinal a été également observé chez 1,5% des individus dans une population d’enfants issus de la communauté. Le phénotype de résistance observé était lié à la production de la carbapénèmase OXA-48. Bien que sept espèces productrices ont été identifiées, la plupart des isolats étaient des souches non-redondantes appartenant aux espèces K. pneumoniae et surtout E. coli. Le vecteur de la diffusion de OXA-48 dans ces bactéries était trois plasmides du groupe d’incompatibilité IncL/M de 49 kb, 63 kb et 84 kb. Cependant, 67% des souches E. coli portaient le gène codant OXA-48 (blaOXA-48) sur le chromosome. La caractérisation des supports génétiques par des approches de séquençage à haut débit a montré qu’ils étaient apparentés et le produit de remaniements génétiques impliquant le transposon Tn21-like, la séquence d'insertion IS1R ou un nouveau transposon composite appelé Tn6237. L’insertion chromosomique de blaOXA-48 résultait de la transposition de Tn6237 qui est capable de transférer un fragment plasmidique de 20 kb dans différents sites du chromosome de E. coli. Les souches K. pneumoniae produisant OXA-48 n’appartenaient pas aux génotypes capsulaires hautement virulents K1 et K2, mais portaient des facteurs identifiées pour favoriser la virulence ou la colonisation de l’hôte. OXA-48 a été observée dans tous les phylogroupes de E. coli, y compris les phylogroupes B2 et D connus pour contenir les souches pathogènes extra-intestinales. Une souche se distinguait par une accumulation sans précédent de huit îlots de pathogénicité. Cette souche induisait une létalité inhabituellement élevée dans un modèle murin de sepsis. En conclusion, l’acquisition du gène blaOXA-48 est donc liée à la diffusion de plasmides apparentés, qui sont marqués par une plasticité conduisant à une localisation chromosomique du gène pouvant favoriser sa persistance. Elle conduit à une diffusion multi-clonale de souches K. pneumoniae et surtout E. coli potentiellement hautement virulentes. Cette association entre de l’espèce E. coli et la carbapénèmase OXA-48 est inquiétante, car E. coli constitue à la fois un réservoir dont la taille peut être considérable et un pathogène responsable d’infections fréquentes pouvant parfois mettre en jeu le pronostic vital.
In the β-lactam…
Advisors/Committee Members: Bonnet, Richard (thesis director), Hamze, Monzer (thesis director).
Subjects/Keywords: Carbapénèmases; Entérobactéries; Génotypes; Carbapenemases; Enterobacteriaceae; Genotypes; 576
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Beyrouthy, R. (2014). Entérobactéries résistantes aux carbapénèmes isolées au Nord Liban : mécanismes, support génétique et pathogénicité : Enterobacteriaceae resistant to carbapenems isolated in North Lebanon : mechanisms, genetic support and pathogenicity. (Doctoral Dissertation). Clermont-Ferrand 1. Retrieved from http://www.theses.fr/2014CLF1MM10
Chicago Manual of Style (16th Edition):
Beyrouthy, Racha. “Entérobactéries résistantes aux carbapénèmes isolées au Nord Liban : mécanismes, support génétique et pathogénicité : Enterobacteriaceae resistant to carbapenems isolated in North Lebanon : mechanisms, genetic support and pathogenicity.” 2014. Doctoral Dissertation, Clermont-Ferrand 1. Accessed April 15, 2021.
http://www.theses.fr/2014CLF1MM10.
MLA Handbook (7th Edition):
Beyrouthy, Racha. “Entérobactéries résistantes aux carbapénèmes isolées au Nord Liban : mécanismes, support génétique et pathogénicité : Enterobacteriaceae resistant to carbapenems isolated in North Lebanon : mechanisms, genetic support and pathogenicity.” 2014. Web. 15 Apr 2021.
Vancouver:
Beyrouthy R. Entérobactéries résistantes aux carbapénèmes isolées au Nord Liban : mécanismes, support génétique et pathogénicité : Enterobacteriaceae resistant to carbapenems isolated in North Lebanon : mechanisms, genetic support and pathogenicity. [Internet] [Doctoral dissertation]. Clermont-Ferrand 1; 2014. [cited 2021 Apr 15].
Available from: http://www.theses.fr/2014CLF1MM10.
Council of Science Editors:
Beyrouthy R. Entérobactéries résistantes aux carbapénèmes isolées au Nord Liban : mécanismes, support génétique et pathogénicité : Enterobacteriaceae resistant to carbapenems isolated in North Lebanon : mechanisms, genetic support and pathogenicity. [Doctoral Dissertation]. Clermont-Ferrand 1; 2014. Available from: http://www.theses.fr/2014CLF1MM10

Univerzitet u Beogradu
28.
Kureljušić, Jasna M., 1981-.
Fenotipizacija, genotipizacija i osetljivost na
antimikrobne lekove Salmonella spp. izolovanih sa trupova zaklanih
svinja.
Degree: Fakultet veterinarske medicine, 2017, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:15740/bdef:Content/get
► Veterinarska medicina - Higijena i tehnologija namirnica - Hygiene and technology of food of animal origin
Cilj ove doktorske disertacije je da se utvrdi zastupljenost…
(more)
▼ Veterinarska medicina - Higijena i tehnologija
namirnica - Hygiene and technology of food of animal
origin
Cilj ove doktorske disertacije je da se utvrdi
zastupljenost salmonela u uzorcima briseva sa trupova svinja u
različitim fazama proizvodnje i sadržaja ileuma, izvrši njihova
fenotipizacija, genotipizacija i utvrdi osetljivost na antimikrobne
lekove. Ispitivanje je sprovedeno na jednoj klanici srednjeg
kapaciteta iz okoline Beograda. Prilikom posete klanici, vršeno je
i anketiranje, odnosno prikupljanje podataka o: poreklu svinja
(farma, otkup), mestu nabavke, načinu ishrane (suva, tečna,
kombinovana), telesnoj masi svinja, starosti, dužini trajanja
transporta, dužini boravka u stočnom depou, eventualnom mešanju
svinja u stočnom depou sa svinjama drugog porekla i higijeni
stočnog depoa. Za ispitivanje su uzorkovani brisevi sa 100 trupova
svinja kao i sadržaj ileuma. Uzorkovanje je trajalo deset nedelja,
svake nedelje se rotirao dan, kako bi se obuhvatili svi dani u
nedelji. Prilikom uzorkovanja, uzimali su se brisevi sa trupova
nakon omamljivanja, zatim nakon završne obrade, a pre hlađenja i
24h posle početka hlađenja. Pored toga, uzorkovano je i 100 uzoraka
sadržaja ileuma od istih trupova. Brisevi su uzimani sa obe
polovine (polutke) istog trupa, što čini 200 uzoraka briseva nakon
omamljivanja, 200 nakon završne obrade i 200 briseva nakon
hlađenja, odnosno ukupno 600 briseva za ispitivanje na prisustvo
salmonela i enterobakterija. Izolacija Salmonella spp. vršena je
prema standardu SRPS EN ISO 6579:2008, Mikrobiologija hrane i hrane
za životinje - Horizontalna metoda za otkrivanje Salmonella spp.
Izolacija Salmonella spp. u uzorcima sadržaja ileuma vršena je
prema istom standardu SRPS EN ISO 6579:2008, Annex D, Otkrivanje
Salmonella spp. u fecesu životinja i u uzorcima iz životne sredine
u primarnoj fazi proizvodnje...
Advisors/Committee Members: Karabasil, Nedeljko, 1971-.
Subjects/Keywords: pigs; slaughter; Enterobacteriaceae; Salmonella;
antimicrobial resistance; PFGE
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kureljušić, Jasna M., 1. (2017). Fenotipizacija, genotipizacija i osetljivost na
antimikrobne lekove Salmonella spp. izolovanih sa trupova zaklanih
svinja. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:15740/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kureljušić, Jasna M., 1981-. “Fenotipizacija, genotipizacija i osetljivost na
antimikrobne lekove Salmonella spp. izolovanih sa trupova zaklanih
svinja.” 2017. Thesis, Univerzitet u Beogradu. Accessed April 15, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:15740/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kureljušić, Jasna M., 1981-. “Fenotipizacija, genotipizacija i osetljivost na
antimikrobne lekove Salmonella spp. izolovanih sa trupova zaklanih
svinja.” 2017. Web. 15 Apr 2021.
Vancouver:
Kureljušić, Jasna M. 1. Fenotipizacija, genotipizacija i osetljivost na
antimikrobne lekove Salmonella spp. izolovanih sa trupova zaklanih
svinja. [Internet] [Thesis]. Univerzitet u Beogradu; 2017. [cited 2021 Apr 15].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:15740/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kureljušić, Jasna M. 1. Fenotipizacija, genotipizacija i osetljivost na
antimikrobne lekove Salmonella spp. izolovanih sa trupova zaklanih
svinja. [Thesis]. Univerzitet u Beogradu; 2017. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:15740/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Autònoma de Barcelona
29.
Grünbaum, Federico.
Resistencia a aminoglucósidos en Enterobacteriaceae.
Degree: Departament de Bioquímica i Biologia Molecular, 2011, Universitat Autònoma de Barcelona
URL: http://hdl.handle.net/10803/42291
► INTRODUCTION: Aminoglycosides have been losing their place in the therapeutic arsenal for combating enterobacterial infections, partly due to the emergence of other antibiotics greater spectrum…
(more)
▼ INTRODUCTION: Aminoglycosides have been losing their place in the therapeutic arsenal for combating enterobacterial infections, partly due to the emergence of other antibiotics greater spectrum and lower toxicity, such as cephalosporins or 3rd and 4th generation fluoroquinolones, and partly the increase of resistance, mainly due to the presence of aminoglycoside modifying enzymes (AME). However, after the emergence of multi-resistant strains for which therapeutic options are very limited, aminoglycosides regained importance in the treatment of infections, especially in hospitals. In the Clinical Microbiology Laboratory aminoglycoside antibiotics represent a group that is difficult for the interpretative reading of disk diffusion antibiogram.
OBJECTIVES: To determine the prevalence of aminoglycoside resistance in
Enterobacteriaceae in our area, the prevalence of AME genes and phenotypic characteristics of the inhibition zone edge. To determine the association between resistance by disk diffusion technique, the presence of AME genes and phenotypic features of the inhibition zone edge, creating rules to assist the interpretative reading of disk diffusion antibiogram of aminoglycosides in
Enterobacteriaceae
METHODS: We performed an observational descriptive study which analyzed 788 isolates of
Enterobacteriaceae isolated between January 1, 2006 and March 31, 2006 at the Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. Study of sensitivity to kanamycin, gentamicin, tobramycin, amikacin, netilmicin, neomycin, streptomycin and spectinomycin by disk diffusion technique, detection of genes aph (3")-Ia, aph (3")-Ib, ant (3")-Ia, aph (3')-Ia, ant (2")-Ia, aac (3)-IIa, aac (6')-Ia, aac (6')-Ib, aac (6')-Ic , aac (3)-Ia, aac (3)-Ib, aac (2')-Ia by the technique of polymerase chain reaction (PCR). Study of clonal relationships of isolates by ERIC-PCR and PFGE.
RESULTS AND CONCLUSIONS: The antimicrobial susceptibility in
Enterobacteriaceae between 2004 and 2006 in the Hospital de la Santa Creu i Sant Pau, Barcelona, Spain has remained stable in the beta-lactam group, while resistance to fluoroquinolones increased up to 30%, and has appeared amikacin resistance, while resistance to gentamicin and tobramycin continued below 10%.
The most prevalent aminoglycoside resistance pattern was caused by aph (3'')-Ib conferring resistance to streptomycin, present in one third of the isolates resistant to one of the aminoglycosides studied. Of patterns affecting aminoglycosides in clinical use, the most prevalent was caused by aac (3)-IIa: resistance to gentamicin and tobramycin, present in 60% of isolates with resistance to aminoglycosides in clinical use.
The presence of phenotypic features in the inhibition zone edge (sharper zone and colonies inside inhibition zone) was significantly associated with the presence of AME genes in the 8 aminoglycosides tested.
Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Mirelis Otero, Beatriz (director), Navarro Risueño, Ferran (director), true (authorsendemail).
Subjects/Keywords: Resistencia; Aminoglucósidos; Enterobacteriaceae; Ciències Experimentals; 579
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Grünbaum, F. (2011). Resistencia a aminoglucósidos en Enterobacteriaceae. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/42291
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Grünbaum, Federico. “Resistencia a aminoglucósidos en Enterobacteriaceae.” 2011. Thesis, Universitat Autònoma de Barcelona. Accessed April 15, 2021.
http://hdl.handle.net/10803/42291.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Grünbaum, Federico. “Resistencia a aminoglucósidos en Enterobacteriaceae.” 2011. Web. 15 Apr 2021.
Vancouver:
Grünbaum F. Resistencia a aminoglucósidos en Enterobacteriaceae. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2011. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/10803/42291.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Grünbaum F. Resistencia a aminoglucósidos en Enterobacteriaceae. [Thesis]. Universitat Autònoma de Barcelona; 2011. Available from: http://hdl.handle.net/10803/42291
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manitoba
30.
Simner, Patricia Jeanne.
Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae with focus on the molecular characterization of ESBL- and AmpC β-lactamase- producing Escherichia coli isolated in Canadian hospitals from 2005-2009.
Degree: Medical Microbiology, 2011, University of Manitoba
URL: http://hdl.handle.net/1993/4412
► The spread of resistance to the cephalosporins in the Enterobacteriaceae and more specifically within E. coli is a continuing cause of public health concern, with…
(more)
▼ The spread of resistance to the cephalosporins in the
Enterobacteriaceae and more specifically within E. coli is a continuing cause of public health concern, with such resistance increasingly seen in community- and nosocomial-acquired infections. Extended-spectrum ß-lactamase (ESBL) and AmpC ß-lactamase (AmpC) enzymes cause most cephalosporin resistance in E. coli by hydrolysis of the antimicrobial and continue to jeopardize patient outcome. The purpose of this thesis was to determine the prevalence of ESBL-producing
Enterobacteriaceae and to molecularly characterize ESBL and AmpC producers found to be associated with the increasing cephalosporin resistance among E. coli within Canadian hospitals from 2005 to 2009.
Isolates were collected as part of the Canadian Intensive Care Unit and Canadian Ward surveillance studies. ESBL and AmpC producers were molecularly characterized for resistance genes, virulence factors and phylogenetic groups. All strains were typed using PFGE and ESBL-producing E. coli were further typed by MLST. Plasmids bearing the ESBL and AmpC genes were characterized by BglII RFLP analysis and a multiplex PCR for replicon typing.
ESBL-producing E. coli and K. pneumoniae and AmpC-producing E. coli were found to be firmly established in Canadian hospitals; whereas, ESBL-producing K. oxytoca and P. mirabilis have yet to emerge. Increasing resistance to several unrelated antimicrobials leading to multi-drug resistance among these pathogens is concerning.
The successful dissemination of ESBL-producing E. coli in Canada occurs through a diversity of different mechanisms and does not correspond to a single ESBL determinant, or a single clone, or a single plasmid but rather through the combination of clonal spread of virulent strains and the acquisition of diverse ESBL-bearing plasmids. However, the predominance of CTX-M-15-producing E. coli in this study was mainly due to the virulent ST131 clone and the diverse IncFII plasmids bearing the blaCTX-M-15 gene. Whereas, horizontal transfer of genetically similar IncI1, IncA/C and IncK/B plasmids bearing blaCMY-2 and the clonal spread of virulent strains, including ST131 with ampC promoter/attenuator mutations, appears to be playing a role in the spread of AmpC-producing E. coli isolates in Canadian hospitals.
The increasing prevalence of these multi-drug resistant pathogens in Canadian hospitals demonstrates the need for increased surveillance and understanding of these emerging pathogens. The continued surveillance will help guide proper infection control procedures and identify optimal treatment of these clinically important pathogens in Canadian hospitals.
Advisors/Committee Members: Hoban, Daryl J. (Medical Microbiology) Zhanel, George G. (Medical Microbiology) (supervisor), Embree, Joanne (Medical Microbiology) Mulvey, Michael R. (Medical Microbiology) Worobec, Elizabeth (Microbiology) (examiningcommittee).
Subjects/Keywords: Beta-Lactamases; Escherichia coli; Canada; Enterobacteriaceae
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Simner, P. J. (2011). Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae with focus on the molecular characterization of ESBL- and AmpC β-lactamase- producing Escherichia coli isolated in Canadian hospitals from 2005-2009. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/4412
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Simner, Patricia Jeanne. “Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae with focus on the molecular characterization of ESBL- and AmpC β-lactamase- producing Escherichia coli isolated in Canadian hospitals from 2005-2009.” 2011. Thesis, University of Manitoba. Accessed April 15, 2021.
http://hdl.handle.net/1993/4412.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Simner, Patricia Jeanne. “Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae with focus on the molecular characterization of ESBL- and AmpC β-lactamase- producing Escherichia coli isolated in Canadian hospitals from 2005-2009.” 2011. Web. 15 Apr 2021.
Vancouver:
Simner PJ. Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae with focus on the molecular characterization of ESBL- and AmpC β-lactamase- producing Escherichia coli isolated in Canadian hospitals from 2005-2009. [Internet] [Thesis]. University of Manitoba; 2011. [cited 2021 Apr 15].
Available from: http://hdl.handle.net/1993/4412.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Simner PJ. Prevalence of extended-spectrum β-lactamase-producing Enterobacteriaceae with focus on the molecular characterization of ESBL- and AmpC β-lactamase- producing Escherichia coli isolated in Canadian hospitals from 2005-2009. [Thesis]. University of Manitoba; 2011. Available from: http://hdl.handle.net/1993/4412
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
◁ [1] [2] [3] [4] [5] … [11] ▶
.