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You searched for subject:(Enterobacter sp YSU). Showing records 1 – 2 of 2 total matches.

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1. Rono, Beatrice C. Identification of a Possible Selenite Sensor Protein from <i>Enterobacter</i> sp. YSU.

Degree: MSin Biology, Department of Biological Sciences, 2013, Youngstown State University

The Y-12 plant (Oak Ridge, TN) contributed to national defense during World War II and the cold war. The plant processed uranium to make nuclear bombs and later switched to lithium processing to make hydrogen bombs. This process resulted in heavy metal waste deposits in East Fork Poplar Creek and the surrounding environment. <i>Enterobacter</i> sp. YSU, which was isolated from this creek, was found to be resistant to metal salts of zinc, cadmium, mercury, selenite, silver, copper and gold. Metal resistant bacteria encode proteins involved in pumping metals out of the cell, in converting them to less toxic forms or in sequestering them. Transposon mutagenesis was used to identify genes involved in selenite resistance. When introduced into the YSU strain, the EZ-Tn5TM<R6K¿ ori/KAN-2> Tnp TransposomeTM inserted itself randomly into the genome of this bacterium. One of the transposome transformants, L31, was sensitive to selenite on agar plates. Minimal inhibitory concentration (MIC) experiments in liquid cultures showed that it was sensitive to 40 mM selenite in LB medium, but not in M-9 minimal medium. Gene rescue, DNA sequencing and Basic Local Alignment Search Tool (BLAST) analysis showed that the interrupted gene product is related to a histidine kinase sensor protein. It may regulate a surrounding gene that encodes a protein involved in efflux of toxic metals. Advisors/Committee Members: Caguiat, Jonathan (Advisor).

Subjects/Keywords: Molecular Biology; Microbiology; Enterobacter sp YSU; Transposon mutagenesis; Histidine kinase

…compared to the multimetal resistant bacterium, Enterobacter sp. YSU, S. maltophilia 02 was more… …glucose 4.2 Bacterial Strains and Transposome Enterobacter sp. YSU was isolated from Poplar… …Enterobacter sp. YSU through transposon mutagenesis. 11 Escherichia coli (E. coli)… …Overnight cultures of L31 mutant and Enterobacter sp. YSU were started in M-9 medium and LB medium… …ml of overnight cultures of Enterobacter sp. YSU and L31 mutant was added to the LB medium… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Rono, B. C. (2013). Identification of a Possible Selenite Sensor Protein from <i>Enterobacter</i> sp. YSU. (Masters Thesis). Youngstown State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ysu1379609443

Chicago Manual of Style (16th Edition):

Rono, Beatrice C. “Identification of a Possible Selenite Sensor Protein from <i>Enterobacter</i> sp. YSU.” 2013. Masters Thesis, Youngstown State University. Accessed January 22, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1379609443.

MLA Handbook (7th Edition):

Rono, Beatrice C. “Identification of a Possible Selenite Sensor Protein from <i>Enterobacter</i> sp. YSU.” 2013. Web. 22 Jan 2020.

Vancouver:

Rono BC. Identification of a Possible Selenite Sensor Protein from <i>Enterobacter</i> sp. YSU. [Internet] [Masters thesis]. Youngstown State University; 2013. [cited 2020 Jan 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1379609443.

Council of Science Editors:

Rono BC. Identification of a Possible Selenite Sensor Protein from <i>Enterobacter</i> sp. YSU. [Masters Thesis]. Youngstown State University; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1379609443

2. Shaik, Nabeel A. Quantitative determination of selenite transformation by <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> OR02.

Degree: MSin Chemistry, Department of Chemistry, 2011, Youngstown State University

The Y-12 plant in Oak Ridge, TN processed uranium and lithium to produce nuclear weapons during World War II and the Cold War. Because the production of nuclear weapons during these time periods was urgent, waste from these processes was not properly contained and nearby East Fork Poplar Creek was contaminated with mercury and other heavy metals. <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> Oak Ridge strain 02 (<i>S. maltophilia</i> 02), which were isolated from East Fork Poplar Creek, are resistant to several heavy metals and selenite, an oxyanion of selenium. The general resistance mechanism appears to be a reduction to elemental selenium. The sodium selenite that is added initially to logarithmically growing culture of these strains is clear in color but turns red when it reaches stationary phase. The change in color could be a result of the reduction of soluble selenite to insoluble elemental selenium. We examined the ability of <i>Enterobacter</i> sp. YSU and <i>S. maltophilia</i> 02 to remove 40 mM selenite and 10 mM selenite, respectively, from their growth. Two cultures were prepared: one was exposed to selenite during early log phase and the control was treated with sterile water. Cells and media were collected at an hourly basis and digested using nitric acid. Then, the amount of selenium in each sample was determined using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). Both strains appeared to sequester elemental selenium, but not at high enough levels to significantly decrease the amount of selenite in the growth medium. Advisors/Committee Members: Caguiat, Jonathan (Advisor).

Subjects/Keywords: Analytical Chemistry; Biology; Environmental Studies; Toxicology; transformation of selenite to elemental selenium; <; i>; Enterobacter<; /i>; sp. YSU; <; i>; Stenotrophomonas maltophilia<; /i>; ORO2; ICP-AES (Inductively Coupled Plasma Atomic Emission Spectroscophy)

…in Klett Units for Enterobacter sp. YSU grown with and 21 without Selenium 3 Turbidity… …Enterobacter sp. YSU obtained 27 using known standards 6 ICP Results of calibration curve for S… …Enterobacter sp. YSU 31 obtained using ICP 8 Results of Selenium Concentrations in S. maltophilia… …maltophilia 02) and Enterobacter sp. YSU. These are both Gram negative bacteria that are… …process data. Growth of S. maltophilia 02 and Enterobacter sp. YSU in the presence of selenite… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Shaik, N. A. (2011). Quantitative determination of selenite transformation by <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> OR02. (Masters Thesis). Youngstown State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329660008

Chicago Manual of Style (16th Edition):

Shaik, Nabeel A. “Quantitative determination of selenite transformation by <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> OR02.” 2011. Masters Thesis, Youngstown State University. Accessed January 22, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329660008.

MLA Handbook (7th Edition):

Shaik, Nabeel A. “Quantitative determination of selenite transformation by <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> OR02.” 2011. Web. 22 Jan 2020.

Vancouver:

Shaik NA. Quantitative determination of selenite transformation by <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> OR02. [Internet] [Masters thesis]. Youngstown State University; 2011. [cited 2020 Jan 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329660008.

Council of Science Editors:

Shaik NA. Quantitative determination of selenite transformation by <i>Enterobacter</i> sp. YSU and <i>Stenotrophomonas maltophilia</i> OR02. [Masters Thesis]. Youngstown State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1329660008

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