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Universitat Autònoma de Barcelona

1. Segarra López, Sergi. Estudios de escalada de dosis sobre los efectos del cloruro de benzalconio intracameral en conejos: un modelo animal de enfermedad endotelial corneal.

Degree: Departament de Medicina i Cirurgia Animals, 2016, Universitat Autònoma de Barcelona

Objective: Find a dose of benzalkonium chloride (BAC) which, injected into the anterior chamber in rabbits, causes a selective damage on the corneal endothelium, without affecting the rest of intraocular structures and thus can be used to induce a repeatable and reproducible animal model of corneal endothelial disease. Material and Methods: First, an ex vivo study was performed using 40 rabbit eyes obtained postmortem, which were classified into 8 groups depending on the injected compound: Control (no injection), Balance salt solution (BSS), and increasing concentrations of BAC (0.005%, 0.01%, 0.025%, 0.05%, 0.1% and 0.2%). Secondly, an in vivo study was performed using 24 New Zealand White rabbits, which were classified into 4 groups: BSS (control group); and 0.025%, 0.05% and 0.1% BAC. Only healthy rabbits and eyes without abnormalities were used. The intracameral injections were made at the corneoscleral limbus using a 27G needle and magnifying loupes. In both studies, follow-up assessments of ophthalmological examination, pachymetry and specular microscopy were performed (at 0, 6, 24 and 48 hours in the ex vivo study; and at 0, 2, 7 and 14 days in the in vivo study). Fourteen days after injection, the rabbits from the in vivo study were euthanized. At the end of each study, corneas were vital-stained and evaluated under the light microscope in order to assess the morphology and viability of the corneal endothelial cells (CECs). In the in vivo study, histopathology of the eye globes was also performed. Results: Ex vivo study: Compared to BSS, the CECs density began to decrease significantly with 0.025% BAC, while the CECs area, the degree of corneal edema and the corneal thickness increased significantly with 0.05%, 0.005% and 0.1% BAC, respectively. BAC concentrations of 0.05% and above caused significant increases in CECs mortality and pleomorphism, compared to control and BSS. In vivo study: Compared to BSS, concentrations of 0.025% BAC and above caused a significant increase in the degree of corneal edema and corneal thickness, and in CECs area and polymeghetism. CECs mortality was significantly higher with 0.05% BAC and above concentrations. The CECs density and hexagonality decreased significantly in all BAC groups. A concentration of 0.1% BAC resulted in a higher number of cases presenting with conjunctival congestion and corneal ulcers. Histopathology revealed no significant alterations affecting the rest of the ocular structures after BAC injection. Conclusions: Intracameral injection of 0.1 ml 0.05% BAC in rabbits causes a selective damage on the corneal endothelium, without affecting the rest of intraocular structures. This technique, developed and validated in an ex vivo and an in vivo study, could be used to induce a repeatable and reproducible animal model of endothelial corneal disease in rabbits. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Leiva Repiso, Marta (director), Peña Giménez, Mª Teresa (director), Leiva Repiso, Marta (tutor), true (authorsendemail).

Subjects/Keywords: Model animal; Modelo animal; Animal model; Clorur de benzalconio; Cloruro de benzalconio; Benzalkonium chloride; Malaltia endotelial corneal; Enfermedad endotelial cornela; Corneal endothelial disease; Ciències de la Salut; 617

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Segarra López, S. (2016). Estudios de escalada de dosis sobre los efectos del cloruro de benzalconio intracameral en conejos: un modelo animal de enfermedad endotelial corneal. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/393871

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Segarra López, Sergi. “Estudios de escalada de dosis sobre los efectos del cloruro de benzalconio intracameral en conejos: un modelo animal de enfermedad endotelial corneal.” 2016. Thesis, Universitat Autònoma de Barcelona. Accessed February 28, 2021. http://hdl.handle.net/10803/393871.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Segarra López, Sergi. “Estudios de escalada de dosis sobre los efectos del cloruro de benzalconio intracameral en conejos: un modelo animal de enfermedad endotelial corneal.” 2016. Web. 28 Feb 2021.

Vancouver:

Segarra López S. Estudios de escalada de dosis sobre los efectos del cloruro de benzalconio intracameral en conejos: un modelo animal de enfermedad endotelial corneal. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2016. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/10803/393871.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Segarra López S. Estudios de escalada de dosis sobre los efectos del cloruro de benzalconio intracameral en conejos: un modelo animal de enfermedad endotelial corneal. [Thesis]. Universitat Autònoma de Barcelona; 2016. Available from: http://hdl.handle.net/10803/393871

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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