subject:(Effectors)

University of Saskatchewan

1. Voth, Kevin Andrew 1991-. Structural Biology of Legionella pneumophila Effectors.

Degree: 2019, University of Saskatchewan

► Legionella pneumophila is a Gram-negative intracellular pathogen that causes Legionnaires’ disease and Pontiac fever in elderly or immunocompromised humans. The ability of Legionella to thrive… (more)

▼ Legionella pneumophila is a Gram-negative intracellular pathogen that causes Legionnaires’ disease and Pontiac fever in elderly or immunocompromised humans. The ability of Legionella to thrive within host cells depends on the Legionella-containing vacuole (LCV) which, in turn, relies on the activity of secreted effector proteins for its formation. Effectors are highly variable in structure and function, and functional redundancy is prevalent among them. Consequently, relating structural data to function provides an attractive avenue of research into molecules which are unlikely to exhibit a phenotype upon gene deletion. Our lab relies on X-ray crystallography for macromolecular structure determination. Structural data may point to a function for the protein of interest, which can be verified using mutagenesis, biochemical assays or some combination thereof. This dissertation explores the structure and putative function of effectors LpnE(lpg2222), MavE(lpg2344) and MavL(lpg2526). LpnE (Legionella pneumophila Entry) is a Sel1-like repeat (SLR) protein implicated in host cell invasion. During infection, a eukaryotic polyphosphate 5-phosphatase, called Oculocerebrorenal syndrome of Lowe protein 1 (OCRL1), is recruited to the LCV by an interaction with LpnE and restricts bacterial replication by an unknown mechanism. The crystal structure of His-LpnE(73-375) reveals a typical SLR super-helix with a concave surface implicated in protein-protein interactions. Herein, critical residues promoting the LpnE-OCRL interaction are uncovered using size exclusion chromatography with multi-angle light scattering (SEC-MALS). In addition, we show that LpnE localizes to cis¬-Golgi using its signal peptide. These findings are compiled into a mechanistic hypothesis where: (1) LpnE localizes to the LCV by its predicted signal peptide. (2) OCRL binding prevents liberation of LpnE from the LCV and (3) renders LpnE unable to promote infection by mediating protein-protein interactions in the cytosol. MavE is one of many proteins identified as a secreted effector based on its ability to rescue LCV localization of a translocation deficient SidC (SidCΔ100). Our collaborator, Dr. Yousef Abu-Kwaik, has obtained a unique phenotype for Δlpg2344 (MavE) mutants, in which the LCV fuses with lysosomes (unpublished data). He suggests that MavE interacts with proteins harbouring phosphotyrosine-binding domains (PTBs) using its NPxY motif. The recruitment of these binding partners may impede autophagic trafficking. The crystal structure of MavE(39-172) presented in this dissertation has an overall structure reminiscent of the grass pollen allergen, Phlp 5b, with the NPxY motif located on a loop of poorly defined electron density. This loop has no counterpart in Phlp 5b and has flexibility that may accommodate protein-protein interactions. These structural data corroborate the proposed role of the NPxY motif while revealing a scaffold domain previously seen in the grass pollen allergen, Phlp 5b. MavL is another secreted effector identified in the same… Advisors/Committee Members: Luo, Yu, Lee, Jeremy, Geyer, Clarence R, Leung, Adelaine.

Subjects/Keywords: Host-pathogen; effectors

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APA (6^th Edition):

Voth, K. A. 1. (2019). Structural Biology of Legionella pneumophila Effectors. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12302

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Voth, Kevin Andrew 1991-. “Structural Biology of Legionella pneumophila Effectors.” 2019. Thesis, University of Saskatchewan. Accessed December 09, 2019. http://hdl.handle.net/10388/12302.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Voth, Kevin Andrew 1991-. “Structural Biology of Legionella pneumophila Effectors.” 2019. Web. 09 Dec 2019.

Vancouver:

Voth KA1. Structural Biology of Legionella pneumophila Effectors. [Internet] [Thesis]. University of Saskatchewan; 2019. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10388/12302.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Voth KA1. Structural Biology of Legionella pneumophila Effectors. [Thesis]. University of Saskatchewan; 2019. Available from: http://hdl.handle.net/10388/12302

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Dundee

2. Griffe, Lucie L. Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley.

Degree: PhD, 2017, University of Dundee

URL: http://hdl.handle.net/10588/72e2cc57-c7fd-4158-991e-03127df74984

► Rhynchosporium commune is one of the most destructive fungal pathogens of barley worldwide. It causes scald, responsible for reduced grain quality and yield losses of… (more)

▼ Rhynchosporium commune is one of the most destructive fungal pathogens of barley worldwide. It causes scald, responsible for reduced grain quality and yield losses of up to 40%. This project aimed to identify genetic resistance in barley using two different approaches: an effector approach through the identification of important pathogen virulence factors and their barley targets, and a genomics association approach. Numerous secreted effectors have been identified in many phytopathogens including R. commune. Rrs1 resistance, recognising the R. commune avirulence protein - AvrRrs1 (NIP1) has been deployed in the field to prevent infection but has soon proven ineffective. R. commune has managed to overcome this resistance by alteration or deletion of the NIP1 gene as it is not essential for pathogenicity. However, our field trial data suggests that Rrs1 remains an important component of resistance to R. commune in the field. Resistance genes recognising more essential Avr genes are likely to be more durable and as a consequence, the discovery of novel R. commune Avr genes is fundamental for the implementation of an integrated pest management approach to prevent this disease. Recent sequencing of the R. commune genome allowed identification of putative effectors. Expression of 26 potential effectors with low sequence variability in 9 sequenced R. commune strains have been analysed during barley infection. The best genes were selected for gene disruption and individual expression in barley cultivars and landraces using the Barley Stripe Mosaic Virus (BSMV) – based expression system to see if they are recognised by the plant. The work also focused on candidate effectors with putative functions. A putative protease inhibitor was chosen for functional characterisation but its function and importance for pathogenicity could not be confirmed. In addition, high amount of the candidate protein appeared to be toxic for barley and Nicotinana benthamiana. Two SA (salicylic acid)-related putative effectors were also chosen for further characterisation and revealed a direct link between the SA pathway of barley and R. commune. The results of this project suggest that R. commune might be able to manipulate the SA pathway of the host confirming the existence of a biotrophic phase of the fungus. The genomics association approach to identify resistance genes against R. commune in barley used a Genome Wide Association Scan (GWAS) using a combination of three years of disease nursery field trial data for a collection of over 500 elite spring barley cultivars. This analysis identified a number of quantitative trait loci (QTL) in barley genome regions previously shown to contain major resistance genes such as Rrs1 on chromosome 3H, Rrs2 on chromosome 7H, Rrs3 on chromosome 4H, Rrs4 on chromosome 3H, Rrs13 on chromosome 6H, Rrs14 on chromosome 1H and Rrs16 on chromosome 4H, as well…

Subjects/Keywords: Barley; Resistance; Genetics; Effectors; Rhynchosporium commune

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Griffe, L. L. (2017). Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley. (Doctoral Dissertation). University of Dundee. Retrieved from http://hdl.handle.net/10588/72e2cc57-c7fd-4158-991e-03127df74984

Chicago Manual of Style (16^th Edition):

Griffe, Lucie L. “Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley.” 2017. Doctoral Dissertation, University of Dundee. Accessed December 09, 2019. http://hdl.handle.net/10588/72e2cc57-c7fd-4158-991e-03127df74984.

MLA Handbook (7^th Edition):

Griffe, Lucie L. “Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley.” 2017. Web. 09 Dec 2019.

Vancouver:

Griffe LL. Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley. [Internet] [Doctoral dissertation]. University of Dundee; 2017. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10588/72e2cc57-c7fd-4158-991e-03127df74984.

Council of Science Editors:

Griffe LL. Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley. [Doctoral Dissertation]. University of Dundee; 2017. Available from: http://hdl.handle.net/10588/72e2cc57-c7fd-4158-991e-03127df74984

University of the Western Cape

3. Nyembe, Nompumelelo Philile Praiseworth. Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors .

Degree: 2014, University of the Western Cape

URL: http://hdl.handle.net/11394/4325

► Xylophilus ampelinus, the causal agent of bacterial blight and canker of grapevines, has long been a threat to the table grape industry in the Western… (more)

▼ Xylophilus ampelinus, the causal agent of bacterial blight and canker of grapevines, has long been a threat to the table grape industry in the Western Cape, leading to severe economic losses due to the reduced productivity and shortened lifespan of infected grapevines. Very little is known about the genetic makeup of the organism, especially with regard to the factors that contribute to its pathogenicity. Generally, bacterial pathogens directly inject the effector proteins into host cells via Type III secretion system (T3SS). In the attempts to identify and characterize the T3 secreted effectors, different reporter plasmid systems have been used to study the secretion and translocation mechanisms the effectors employ during pathogenicity. The aim of the study was to generate a T3 reporter plasmid system for X. ampelinus that will allow the identification and classification of potential pathogenicity factors as members of the Type III secretion class of effectors. First, the avrBs1 family genes avrBs1 and avrA were identified and characterized. The two avirulence genes induced HR on Nicotiana tabacum leaves. Due to the relatedness of the X. ampelinus avr sequences to those of xanthomonads, and the fact that Xanthomonas avrBs1 has been successfully used in a number T3 effector studies, it was decided to construct an X. ampelinus T3 effector reporter vector based on the avrBs1 gene. The minimal segment of the X. ampelinus AvrBs1 protein C-terminus, sufficient for recognition inside host cells and also responsible for HR-induction was identified and characterized using Agrobacterium-mediated transient expression. The AvrBs157-413 HR-inducing domain was cloned in-frame with the 3x FLAG epitope, into a broad-host range vector. To test the reporter vector, the full length avrBs1 sequences of X. ampelinus and Xanthomonas campestris pv. campestris were cloned ahead of the 3x FLAG epitope and the constructs were transferred into XaΔavrBs1 knockout mutant to test for protein secretion. Furthermore, the reporter construct was tested for Type III protein translocation on Bs1 resistant pepper cultivar STAR 6657. Optimization of protein secretion and translocation assays is however required for the improved results. This might include the application of an alternative protein tag to identify candidate X. ampelinus T3SS effectors. Advisors/Committee Members: Ndimba, Bongani K (advisor), Petersen, Yolanda (advisor).

Subjects/Keywords: Vitis vinifera; Xylophilus ampelinus; Bacterial effectors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nyembe, N. P. P. (2014). Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nyembe, Nompumelelo Philile Praiseworth. “Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors .” 2014. Thesis, University of the Western Cape. Accessed December 09, 2019. http://hdl.handle.net/11394/4325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nyembe, Nompumelelo Philile Praiseworth. “Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors .” 2014. Web. 09 Dec 2019.

Vancouver:

Nyembe NPP. Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors . [Internet] [Thesis]. University of the Western Cape; 2014. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/11394/4325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nyembe NPP. Development of a reporter system for the analysis of xylophilus ampelinus type III secreted effectors . [Thesis]. University of the Western Cape; 2014. Available from: http://hdl.handle.net/11394/4325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

4. Kale, Shiv Dutt. How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection.

Degree: PhD, Genetics, Bioinformatics, and Computational Biology, 2011, Virginia Tech

URL: http://hdl.handle.net/10919/77375

► The genus Phytophthora contains a large number of species that are known plant pathogens of a variety of important crops. Phytophthora sojae, a hemibiotroph, causes… (more)

Subjects/Keywords: Effectors; Oomycete; Fungi; Phosphatidylinositol-3-phosphate; RXLR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kale, S. D. (2011). How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77375

Chicago Manual of Style (16^th Edition):

Kale, Shiv Dutt. “How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection.” 2011. Doctoral Dissertation, Virginia Tech. Accessed December 09, 2019. http://hdl.handle.net/10919/77375.

MLA Handbook (7^th Edition):

Kale, Shiv Dutt. “How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection.” 2011. Web. 09 Dec 2019.

Vancouver:

Kale SD. How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection. [Internet] [Doctoral dissertation]. Virginia Tech; 2011. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10919/77375.

Council of Science Editors:

Kale SD. How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection. [Doctoral Dissertation]. Virginia Tech; 2011. Available from: http://hdl.handle.net/10919/77375

University of Dundee

5. Griffe, Lucie L. Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley.

Degree: PhD, 2017, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/72e2cc57-c7fd-4158-991e-03127df74984 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.743118

► Rhynchosporium commune is one of the most destructive fungal pathogens of barley worldwide. It causes scald, responsible for reduced grain quality and yield losses of… (more)

▼ Rhynchosporium commune is one of the most destructive fungal pathogens of barley worldwide. It causes scald, responsible for reduced grain quality and yield losses of up to 40%. This project aimed to identify genetic resistance in barley using two different approaches: an effector approach through the identification of important pathogen virulence factors and their barley targets, and a genomics association approach. Numerous secreted effectors have been identified in many phytopathogens including R. commune. Rrs1 resistance, recognising the R. commune avirulence protein - AvrRrs1 (NIP1) has been deployed in the field to prevent infection but has soon proven ineffective. R. commune has managed to overcome this resistance by alteration or deletion of the NIP1 gene as it is not essential for pathogenicity. However, our field trial data suggests that Rrs1 remains an important component of resistance to R. commune in the field. Resistance genes recognising more essential Avr genes are likely to be more durable and as a consequence, the discovery of novel R. commune Avr genes is fundamental for the implementation of an integrated pest management approach to prevent this disease. Recent sequencing of the R. commune genome allowed identification of putative effectors. Expression of 26 potential effectors with low sequence variability in 9 sequenced R. commune strains have been analysed during barley infection. The best genes were selected for gene disruption and individual expression in barley cultivars and landraces using the Barley Stripe Mosaic Virus (BSMV) – based expression system to see if they are recognised by the plant. The work also focused on candidate effectors with putative functions. A putative protease inhibitor was chosen for functional characterisation but its function and importance for pathogenicity could not be confirmed. In addition, high amount of the candidate protein appeared to be toxic for barley and Nicotinana benthamiana. Two SA (salicylic acid)-related putative effectors were also chosen for further characterisation and revealed a direct link between the SA pathway of barley and R. commune. The results of this project suggest that R. commune might be able to manipulate the SA pathway of the host confirming the existence of a biotrophic phase of the fungus. The genomics association approach to identify resistance genes against R. commune in barley used a Genome Wide Association Scan (GWAS) using a combination of three years of disease nursery field trial data for a collection of over 500 elite spring barley cultivars. This analysis identified a number of quantitative trait loci (QTL) in barley genome regions previously shown to contain major resistance genes such as Rrs1 on chromosome 3H, Rrs2 on chromosome 7H, Rrs3 on chromosome 4H, Rrs4 on chromosome 3H, Rrs13 on chromosome 6H, Rrs14 on chromosome 1H and Rrs16 on chromosome 4H, as well…

Subjects/Keywords: 580; Barley; Resistance; Genetics; Effectors; Rhynchosporium commune

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Griffe, L. L. (2017). Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/72e2cc57-c7fd-4158-991e-03127df74984 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.743118

Chicago Manual of Style (16^th Edition):

Griffe, Lucie L. “Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley.” 2017. Doctoral Dissertation, University of Dundee. Accessed December 09, 2019. https://discovery.dundee.ac.uk/en/studentTheses/72e2cc57-c7fd-4158-991e-03127df74984 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.743118.

MLA Handbook (7^th Edition):

Griffe, Lucie L. “Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley.” 2017. Web. 09 Dec 2019.

Vancouver:

Griffe LL. Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley. [Internet] [Doctoral dissertation]. University of Dundee; 2017. [cited 2019 Dec 09]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/72e2cc57-c7fd-4158-991e-03127df74984 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.743118.

Council of Science Editors:

Griffe LL. Applying effectoromics and genomics to identify resistance against Rhynchosporium commune in barley. [Doctoral Dissertation]. University of Dundee; 2017. Available from: https://discovery.dundee.ac.uk/en/studentTheses/72e2cc57-c7fd-4158-991e-03127df74984 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.743118

University of Louisville

6. Kuppireddy, Venkata Swathi. Identification and functional characterization of effectors from an anther smut fungus, Microbotryum lychnidis-dioicae.

Degree: PhD, 2018, University of Louisville

URL: 10.18297/etd/3078 ; https://ir.library.louisville.edu/etd/3078

► Microbotryum lychnidis-dioicae causes anther smut fungus in its host plant, Silene latifolia. The goal of this work is to identify and characterize the virulence… (more)

▼ Microbotryum lychnidis-dioicae causes anther smut fungus in its host plant, Silene latifolia. The goal of this work is to identify and characterize the virulence determinants of this pathogen to better understand the molecular basis behind this host-pathogen interaction. This work studied for the first time the key effectors in the mechanism of infection by this fungal species. Using, yeast two-hybrid screens, I have identified the host plant interaction partners for the effector, MVLG_01732. A second effector MVLG_05720, interacts with other fungal proteins that appear to facilitate the fungal establishment and colonization during the infection. Our findings indicate that a third effector, MVLG_04106, could serve as a transcriptional regulator to promote infection. To further characterize the role of the effector, MVLG_01732, I have conducted heterologous expression studies in A. thaliana followed by infection assays with the pathogen Pseudomonas syringae. Our results indicate that this effector has a role in the early bolting of flowers in A. thaliana, this finding provides an important clue about the role of MVLG_01732 in advancing host plant floral development, which is known to occur in infected S. latifolia flowers. Here, we have identified a key molecular link between a fungal effector and the developmental change it triggers in the host plant. Infection assays reveal that this effector might play a role in promoting pathogen growth. I have also examined the response to this effector by the host by expressing the His-tagged effector in S. latifolia in an experiment designed to model the mechanism of infection in the native habitat. This is the first time that this approach of delivering the candidate effector protein has been carried out in planta and aimed to provide information about the previously unidentified interacting partners from the host. Overall, this dissertation body aimed to increase the number of available genetic tools to study M. lychnidis-dioicae and will serve as a valuable resource for future investigators along with furthering our understanding of the infection mechanism. Advisors/Committee Members: Perlin, Michael, Schultz, David, Schultz, David, Wattenberg, Brian, Klinge, Carolyn, Himes, Paul.

Subjects/Keywords: anther smut; Microbotryum lychnidis-dioicae; effectors; host targets for effectors; biotrophic fungal pathogen; silene latifolia; Molecular Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuppireddy, V. S. (2018). Identification and functional characterization of effectors from an anther smut fungus, Microbotryum lychnidis-dioicae. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/3078 ; https://ir.library.louisville.edu/etd/3078

Chicago Manual of Style (16^th Edition):

Kuppireddy, Venkata Swathi. “Identification and functional characterization of effectors from an anther smut fungus, Microbotryum lychnidis-dioicae.” 2018. Doctoral Dissertation, University of Louisville. Accessed December 09, 2019. 10.18297/etd/3078 ; https://ir.library.louisville.edu/etd/3078.

MLA Handbook (7^th Edition):

Kuppireddy, Venkata Swathi. “Identification and functional characterization of effectors from an anther smut fungus, Microbotryum lychnidis-dioicae.” 2018. Web. 09 Dec 2019.

Vancouver:

Kuppireddy VS. Identification and functional characterization of effectors from an anther smut fungus, Microbotryum lychnidis-dioicae. [Internet] [Doctoral dissertation]. University of Louisville; 2018. [cited 2019 Dec 09]. Available from: 10.18297/etd/3078 ; https://ir.library.louisville.edu/etd/3078.

Council of Science Editors:

Kuppireddy VS. Identification and functional characterization of effectors from an anther smut fungus, Microbotryum lychnidis-dioicae. [Doctoral Dissertation]. University of Louisville; 2018. Available from: 10.18297/etd/3078 ; https://ir.library.louisville.edu/etd/3078

7. Varner, Chad. Developing synthetic multivalent cellular effectors.

Degree: PhD, Chemical and Biomolecular Engineering, 2017, Georgia Tech

URL: http://hdl.handle.net/1853/58743

► I have designed bioconjugates that can elicit desired cellular responses – cellular effectors. Using multivalent interactions, involving the simultaneous binding of multiple receptors to multiple… (more)

Subjects/Keywords: Multivalency; Vaccines; Effectors

…bioconjugates that can elicit desired cellular responses – cellular effectors. I have been… …multivalent cellular effectors to use as vaccines and to direct stem cell fate. First, I have… …Multivalency: Cellular Effectors Another niche that multivalent molecules fill is that of cellular… …effectors. As exemplified in B-cell-antigen interactions (Figure 2), many cellular… …as cellular effectors. (A) VEGF-peptide amphiphiles (VEGF-PA) were…

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Varner, C. (2017). Developing synthetic multivalent cellular effectors. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/58743

Chicago Manual of Style (16^th Edition):

Varner, Chad. “Developing synthetic multivalent cellular effectors.” 2017. Doctoral Dissertation, Georgia Tech. Accessed December 09, 2019. http://hdl.handle.net/1853/58743.

MLA Handbook (7^th Edition):

Varner, Chad. “Developing synthetic multivalent cellular effectors.” 2017. Web. 09 Dec 2019.

Vancouver:

Varner C. Developing synthetic multivalent cellular effectors. [Internet] [Doctoral dissertation]. Georgia Tech; 2017. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/1853/58743.

Council of Science Editors:

Varner C. Developing synthetic multivalent cellular effectors. [Doctoral Dissertation]. Georgia Tech; 2017. Available from: http://hdl.handle.net/1853/58743

University of Hawaii – Manoa

8. Gumtow, Rebecca. Functional Characterization of Putative Extracellular Cystatins, PpmEPICs, in Pathogenicity of Phytophthora palmivora on Papaya.

Degree: 2017, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/51166

►

M.S. University of Hawaii at Manoa 2015.

Phytophthora palmivora is an economically important oomycete pathogen that infects many tropical and subtropical plant species including papaya.… (more)

Subjects/Keywords: Phytophthora palmivora; oomycetes; plant defense; cystatins; EPICs; effectors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gumtow, R. (2017). Functional Characterization of Putative Extracellular Cystatins, PpmEPICs, in Pathogenicity of Phytophthora palmivora on Papaya. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/51166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gumtow, Rebecca. “Functional Characterization of Putative Extracellular Cystatins, PpmEPICs, in Pathogenicity of Phytophthora palmivora on Papaya.” 2017. Thesis, University of Hawaii – Manoa. Accessed December 09, 2019. http://hdl.handle.net/10125/51166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gumtow, Rebecca. “Functional Characterization of Putative Extracellular Cystatins, PpmEPICs, in Pathogenicity of Phytophthora palmivora on Papaya.” 2017. Web. 09 Dec 2019.

Vancouver:

Gumtow R. Functional Characterization of Putative Extracellular Cystatins, PpmEPICs, in Pathogenicity of Phytophthora palmivora on Papaya. [Internet] [Thesis]. University of Hawaii – Manoa; 2017. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10125/51166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gumtow R. Functional Characterization of Putative Extracellular Cystatins, PpmEPICs, in Pathogenicity of Phytophthora palmivora on Papaya. [Thesis]. University of Hawaii – Manoa; 2017. Available from: http://hdl.handle.net/10125/51166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Lelogeais, Virginie. Étude de l’interaction entre L. pneumophila et l’autophagie de la cellule hôte : Study of the interaction between L. pneumophila and host cell autophagy.

Degree: Docteur es, Infectiologie, 2016, Lyon

URL: http://www.theses.fr/2016LYSE1168

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L. pneumophila est l'agent responsable de la légionellose, une pneumonie sévère associée à 10% de mortalité. Cette bactérie intracellulaire a acquis la capacité de survivre… (more)

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L. pneumophila est l'agent responsable de la légionellose, une pneumonie sévère associée à 10% de mortalité. Cette bactérie intracellulaire a acquis la capacité de survivre et de se répliquer dans des cellules humaines. Notamment, L. pneumophila sécrète un grand nombre d'effecteurs par son système de sécrétion de type IV, qui interagissent avec différentes voies cellulaires, dont l'autophagie. L'autophagie est une voie de dégradation conservée qui permet aux cellules eucaryotes de réguler l'homéostasie cellulaire et d'éliminer les agents pathogènes intracellulaires. Néanmoins, nombre d'entre eux ont évolué pour manipuler cette voie à leur propre avantage. Même si l'interaction entre L. pneumophila et l'autophagie a été rapportée, aucun modèle clair n'est déterminé. Dans cette étude, nous montrons qu'une infection à L. pneumophila induit une stimulation globale de l'autophagie, mais que ce phénotype dépend des souches utilisées, et notamment de la présence de certains effecteurs. De plus, l'inhibition de l'autophagie est liée à un défaut de réplication intracellulaire suggérant que cette voie est bénéfique à la bactérie. Afin de rechercher les déterminants génétiques impliqués dans cette interaction, nous avons identifié des effecteurs communs sécrétés par le système de sécrétion de type IV entre L. pneumophila et Coxiella burnetii, une bactérie de l'ordre des Legionellales connue pour stimuler et détourner l'autophagie. La capacité des mutants de ces effecteurs à stimuler l'autophagie chez L. pneumophila a été analysée. Si aucun d'entre eux ne semble impliqué dans la modulation de l'autophagie, cette étude suggère d'autres fonctions pour ces effecteurs conservés

Legionella pneumophila is responsible for the legionellosis disease, a severe pneumonia associated with 10% mortality rate. This intracellular bacterium has evolved the ability to survive and replicate within human cells. Notably, L. pneumophila secretes a high number of type IV secretion system effectors that interfere with many cellular pathways including autophagy. Autophagy, a highly conserved degradative pathway, allows eukaryotic cells to regulate cell homeostasis and fight intracellular pathogens. Nevertheless numerous microorganisms have evolved strategies to subvert this mechanism to their own advantage. The interaction between L. pneumophila and autophagy has been reported but remains unclear. In this study, we show that L. pneumophila infection induces a global stimulation of autophagy, but importantly this autophagy stimulation depends on the bacterial strain. Moreover, we also observed that inhibition of autophagy results in decreased intracellular bacterial proliferation suggesting that host cell autophagy is benificial for L. pneumophila. In order to decipher the molecular determinants involved in the interaction with autophagy, we identified common effectors secreted by the type IV secretion system between L. pneumophila and Coxiella burnetii, a bacterium from the order Legionellale responsible for Q fever and known to stimulate and hijack…

Advisors/Committee Members: Doublet, Patricia (thesis director), Vavre, Fabrice (thesis director).

Subjects/Keywords: Legionella; Autophagie; Infection; Effecteurs; Legionella; Autophagy; Infection; Effectors; 571.6

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APA (6^th Edition):

Lelogeais, V. (2016). Étude de l’interaction entre L. pneumophila et l’autophagie de la cellule hôte : Study of the interaction between L. pneumophila and host cell autophagy. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2016LYSE1168

Chicago Manual of Style (16^th Edition):

Lelogeais, Virginie. “Étude de l’interaction entre L. pneumophila et l’autophagie de la cellule hôte : Study of the interaction between L. pneumophila and host cell autophagy.” 2016. Doctoral Dissertation, Lyon. Accessed December 09, 2019. http://www.theses.fr/2016LYSE1168.

MLA Handbook (7^th Edition):

Lelogeais, Virginie. “Étude de l’interaction entre L. pneumophila et l’autophagie de la cellule hôte : Study of the interaction between L. pneumophila and host cell autophagy.” 2016. Web. 09 Dec 2019.

Vancouver:

Lelogeais V. Étude de l’interaction entre L. pneumophila et l’autophagie de la cellule hôte : Study of the interaction between L. pneumophila and host cell autophagy. [Internet] [Doctoral dissertation]. Lyon; 2016. [cited 2019 Dec 09]. Available from: http://www.theses.fr/2016LYSE1168.

Council of Science Editors:

Lelogeais V. Étude de l’interaction entre L. pneumophila et l’autophagie de la cellule hôte : Study of the interaction between L. pneumophila and host cell autophagy. [Doctoral Dissertation]. Lyon; 2016. Available from: http://www.theses.fr/2016LYSE1168

Iowa State University

10. Rutter, William Brock. Identification and characterization of effectors secreted from sedentary endoparasitic phytonematodes.

Degree: 2013, Iowa State University

URL: https://lib.dr.iastate.edu/etd/13397

► Sedentary endoparasitic phytonematodes are a group of taxa comprised of cyst nematodes (Heterodera and Globodera spp.) and root-knot nematodes (Meloidogyne spp. ), which are some… (more)

Subjects/Keywords: Effectors; Globodera; Heterodera; Meloidogyne; Agricultural Science; Agriculture; Genetics; Plant Pathology

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APA (6^th Edition):

Rutter, W. B. (2013). Identification and characterization of effectors secreted from sedentary endoparasitic phytonematodes. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/13397

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rutter, William Brock. “Identification and characterization of effectors secreted from sedentary endoparasitic phytonematodes.” 2013. Thesis, Iowa State University. Accessed December 09, 2019. https://lib.dr.iastate.edu/etd/13397.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rutter, William Brock. “Identification and characterization of effectors secreted from sedentary endoparasitic phytonematodes.” 2013. Web. 09 Dec 2019.

Vancouver:

Rutter WB. Identification and characterization of effectors secreted from sedentary endoparasitic phytonematodes. [Internet] [Thesis]. Iowa State University; 2013. [cited 2019 Dec 09]. Available from: https://lib.dr.iastate.edu/etd/13397.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rutter WB. Identification and characterization of effectors secreted from sedentary endoparasitic phytonematodes. [Thesis]. Iowa State University; 2013. Available from: https://lib.dr.iastate.edu/etd/13397

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

11. Shybut, Mikel. Transcription Activator-Like (TAL) Effectors of the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis.

Degree: Plant Biology, 2015, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/1g52w36q

► Cassava is an essential food crop relied on by hundreds of millions of people worldwide. Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of… (more)

▼ Cassava is an essential food crop relied on by hundreds of millions of people worldwide. Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB) and the leading destructive bacterial pathogen of cassava. Xam utilizes a unique class of type three effectors known as transcription activator-like (TAL) effectors (TALEs) to activate specific host genes that contribute to virulence and bacterial growth. In Xam, TALEs are often localized to plasmids. TALE-containing plasmids of other Xanthomonas ssp. have been shown to be conjugative, providing a mechanism for the horizontal transfer of TALE virulence components. Here, I characterize the two-TALE containing plasmid pXam46 of Xam isolate CIO151, providing a full draft sequence, TALE virulence assays, and evidence for its mobilizing ability. The potential horizontal transfer of TALEs suggests that TALEs which confer a strong virulence phenotype may be well conserved amongst Xam communities. I screened a subset of 22 global Xam isolates spanning 28 years of evolution from 5 countries over 3 continents for their TALE repertoires. I identified one pair of highly conserved TALEs, including a single repeat variable diresidue (RVD) variant of a pXam46 localized TALE, and 3 additional well-conserved TALEs. Of the two highly conserved TALEs, both contribute to bacterial growth in planta and one is associated with a water soaking disease phenotype. The remaining 3 well-conserved TALEs did not show any measurable contributions to virulence. Some plants contain an evolutionary mechanism to defend against TALEs, carrying executor resistance (R) genes containing TALE binding elements upstream of disease resistance genes. I found that the highly conserved TALE of pXam46 triggers a specific, transcriptionally dependent HR-like phenotype in the non-host Nicotiana benthamiana. Employing RNA-seq, I have identified a list of candidate TALE-upregulated genes that may be involved in the defense response of N. benthamiana. Identifying TALE-triggered R genes as well as conserved TALEs and their susceptibility targets can assist in the design of durable resistance strategies against Xam. Successful strategies may include stacking promoters of multiple conserved TALEs in front of R genes or modifying cassava susceptibility gene promoters to abrogate TALE binding. It is my hope that the basic biology described herein may assist in those efforts.

Subjects/Keywords: Plant pathology; cassava bacterial blight; plasmids; TAL effectors; TALEs; Xanthomonas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shybut, M. (2015). Transcription Activator-Like (TAL) Effectors of the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/1g52w36q

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shybut, Mikel. “Transcription Activator-Like (TAL) Effectors of the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis.” 2015. Thesis, University of California – Berkeley. Accessed December 09, 2019. http://www.escholarship.org/uc/item/1g52w36q.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shybut, Mikel. “Transcription Activator-Like (TAL) Effectors of the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis.” 2015. Web. 09 Dec 2019.

Vancouver:

Shybut M. Transcription Activator-Like (TAL) Effectors of the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis. [Internet] [Thesis]. University of California – Berkeley; 2015. [cited 2019 Dec 09]. Available from: http://www.escholarship.org/uc/item/1g52w36q.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shybut M. Transcription Activator-Like (TAL) Effectors of the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis. [Thesis]. University of California – Berkeley; 2015. Available from: http://www.escholarship.org/uc/item/1g52w36q

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

12. Soyer, Jessica. Caractérisation de la régulation de l’expression des gènes codant des effecteurs chez Leptosphaeria maculans : Regulation of effector gene expression in Leptosphaeria maculans.

Degree: Docteur es, Biologie, 2013, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2013PA112266

► Leptosphaeria maculans ‘brassicae’ (Lmb) est un ascomycète de la classe des Dothideomycètes faisant partie d’un complexe d’espèces présentant différents niveaux d’adaptation au colza. Lmb est… (more)

▼ Leptosphaeria maculans ‘brassicae’ (Lmb) est un ascomycète de la classe des Dothideomycètes faisant partie d’un complexe d’espèces présentant différents niveaux d’adaptation au colza. Lmb est responsable d’une des maladies les plus dommageables sur colza : la nécrose du collet. Lmb présente un cycle de vie complexe au cours duquel il alterne différents modes de vie, traduisant l’existence de mécanismes de régulation fine de l’expression des gènes lui permettant de s’adapter rapidement à de nouvelles conditions. Le séquençage de son génome a révélé une structure originale, avec l’alternance de deux types de régions : les isochores GC et les isochores AT. Alors que les isochores GC sont riches en gènes, les isochores AT sont pauvres en gènes et présentent des caractéristiques de l’hétérochromatine (régions génomiques riches en éléments transposables et présentant un faible taux de recombinaison). Bien que pauvres en gènes, les isochores AT représentent une « niche écologique » pour les gènes codant des effecteurs puisque 20 % des gènes des isochores AT codent des effecteurs putatifs contre seulement 4 % des gènes localisés en isochores GC. Les gènes codant des effecteurs situés en isochores AT présentent un comportement transcriptionnel différent de ceux localisés en isochores GC : une faible expression pendant la croissance mycélienne et une forte induction d’expression pendant l’infection primaire du colza. Sur la base de ces observations, l’objectif de ma thèse était de caractériser le déterminisme de la co-expression des effecteurs situés dans les isochores AT et en particulier d’évaluer si la régulation de l’expression de ces gènes se fait par un contrôle épigénétique lié à leur localisation particulière et/ou par l’intervention de régulateurs communs. Afin de déterminer le rôle de la structure des isochores AT, l’analyse fonctionnelle de protéines impliquées dans le remodelage de la chromatine (i.e. HP1, DIM-5 et DMM-1) a été réalisée et leur implication dans la régulation de l’ensemble des gènes prédits dans le génome de L. maculans a été évaluée. Cette étude a permis de démontrer l’implication de la structure hétérochromatinienne des isochores AT dans la répression de l’expression pendant la croissance mycélienne des gènes situés dans cet environnement génomique, en particulier les gènes codant des effecteurs. Parmi les gènes sous contrôle épigénétique, nous avons pu observer qu’en plus des gènes localisés en isochores AT, des zones en isochores GC étaient aussi affectées et pouvaient constituer des « hot-spots » de contrôle épigénétique. Afin d’identifier des régulateurs candidats pouvant être impliqués dans le contrôle de l’expression des effecteurs pendant l’infection, le répertoire des gènes codant des facteurs de transcription (FTs) chez Lmb a été établi et l’analyse de la conservation de ce répertoire parmi les autres espèces du complexe d’espèces Leptosphaeria a permis d’identifier les FTs spécifiques, ou spécifiquement sur-exprimés pendant l’infection du colza, chez Lmb. Des candidats ont été… Advisors/Committee Members: Fudal, Isabelle (thesis director).

Subjects/Keywords: Hétérochromatine; Effecteurs; Épigénétique; Facteurs de transcription; Heterochromatin; Effectors; Epigenetic; Transcription factor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Soyer, J. (2013). Caractérisation de la régulation de l’expression des gènes codant des effecteurs chez Leptosphaeria maculans : Regulation of effector gene expression in Leptosphaeria maculans. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2013PA112266

Chicago Manual of Style (16^th Edition):

Soyer, Jessica. “Caractérisation de la régulation de l’expression des gènes codant des effecteurs chez Leptosphaeria maculans : Regulation of effector gene expression in Leptosphaeria maculans.” 2013. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed December 09, 2019. http://www.theses.fr/2013PA112266.

MLA Handbook (7^th Edition):

Soyer, Jessica. “Caractérisation de la régulation de l’expression des gènes codant des effecteurs chez Leptosphaeria maculans : Regulation of effector gene expression in Leptosphaeria maculans.” 2013. Web. 09 Dec 2019.

Vancouver:

Soyer J. Caractérisation de la régulation de l’expression des gènes codant des effecteurs chez Leptosphaeria maculans : Regulation of effector gene expression in Leptosphaeria maculans. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2013. [cited 2019 Dec 09]. Available from: http://www.theses.fr/2013PA112266.

Council of Science Editors:

Soyer J. Caractérisation de la régulation de l’expression des gènes codant des effecteurs chez Leptosphaeria maculans : Regulation of effector gene expression in Leptosphaeria maculans. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2013. Available from: http://www.theses.fr/2013PA112266

Swedish University of Agricultural Sciences

13. Njoroge, Anne. Population structure and pathogenicity evolution of Phytophthora infestans affects epidemiology and management of late blight disease.

Degree: 2019, Swedish University of Agricultural Sciences

URL: https://pub.epsilon.slu.se/15976/

► Sound management of late blight, the disease caused by the notorious oomycete pathogen Phytophthora infestans (Mont.) de Bary, is dependent on the pathogen’s population biology.… (more)

▼ Sound management of late blight, the disease caused by the notorious oomycete pathogen Phytophthora infestans (Mont.) de Bary, is dependent on the pathogen’s population biology. However, for P. infestans population structure to give guidance for disease management, successful information flow between the researchers and the practitioners is paramount. We analysed the population in eastern-Africa to determine the pathogen genotypes present in the region. We characterized the isolates using microsatellite markers and mitochondrial DNA haplotypes to enable comparisons with global populations. A European lineage, 2_A1 was found to be dominating the population in eastern-Africa. In addition, the 2_A1 lineage was found to be more aggressive in terms of lesion size, latent periods and incubation periods when compared to the old US-1 lineage. We thus concluded that the tested aggressiveness traits could have partly contributed to the quick displacement of US-1 by 2_A1 in the region. In a study predicting host durability of a genetically engineered potato with a stack of three resistance genes as well as a conventionally bred potato with a stack of five resistance genes, the assessment of pathogen effector genes proved valuable to deduce which of the R-genes were functional in the field. From the effector study, it can be concluded that effector genes in target local P. infestans populations should inform selection of breeding materials since globally, pathogen populations are very diverse. An assessment of commonly grown potato cultivars in eastern-Africa to quantify their susceptibility to late blight in the field found out that nearly all cultivars had partial resistance to P. infestans. The growers’ choice of cultivars is to high degree governed by market demands. Unfortunately, many cultivars with good resistance to late blight have other undesirable agronomic traits hence the rationale behind growing cultivars that are highly susceptible to late blight. Disease management practices, host durability prediction tools and potato breeding approaches should be suitably adjusted to the existing pathogen population.

Subjects/Keywords: late blight; SSR-genotyping; gene pyramiding; effectors; host resistance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Njoroge, A. (2019). Population structure and pathogenicity evolution of Phytophthora infestans affects epidemiology and management of late blight disease. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from https://pub.epsilon.slu.se/15976/

Chicago Manual of Style (16^th Edition):

Njoroge, Anne. “Population structure and pathogenicity evolution of Phytophthora infestans affects epidemiology and management of late blight disease.” 2019. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed December 09, 2019. https://pub.epsilon.slu.se/15976/.

MLA Handbook (7^th Edition):

Njoroge, Anne. “Population structure and pathogenicity evolution of Phytophthora infestans affects epidemiology and management of late blight disease.” 2019. Web. 09 Dec 2019.

Vancouver:

Njoroge A. Population structure and pathogenicity evolution of Phytophthora infestans affects epidemiology and management of late blight disease. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2019. [cited 2019 Dec 09]. Available from: https://pub.epsilon.slu.se/15976/.

Council of Science Editors:

Njoroge A. Population structure and pathogenicity evolution of Phytophthora infestans affects epidemiology and management of late blight disease. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2019. Available from: https://pub.epsilon.slu.se/15976/

University of Ottawa

14. Ndikumana, Steve. Genome Sequencing of the Relevant Zebrafish-Infecting Microsporidian Pseudoloma neurophilia Reveals Atypical Genome Dynamics .

Degree: 2016, University of Ottawa

URL: http://hdl.handle.net/10393/34535

► Since their first discovery in the 19th century, microsporidian species have been found to be successful obligate intracellular parasites capable of infecting a wide variety… (more)

Subjects/Keywords: Pseudoloma neurophilia; Danio rerio; Microsporidia; Transposable elements; Effectors; RNA interference

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ndikumana, S. (2016). Genome Sequencing of the Relevant Zebrafish-Infecting Microsporidian Pseudoloma neurophilia Reveals Atypical Genome Dynamics . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/34535

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ndikumana, Steve. “Genome Sequencing of the Relevant Zebrafish-Infecting Microsporidian Pseudoloma neurophilia Reveals Atypical Genome Dynamics .” 2016. Thesis, University of Ottawa. Accessed December 09, 2019. http://hdl.handle.net/10393/34535.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ndikumana, Steve. “Genome Sequencing of the Relevant Zebrafish-Infecting Microsporidian Pseudoloma neurophilia Reveals Atypical Genome Dynamics .” 2016. Web. 09 Dec 2019.

Vancouver:

Ndikumana S. Genome Sequencing of the Relevant Zebrafish-Infecting Microsporidian Pseudoloma neurophilia Reveals Atypical Genome Dynamics . [Internet] [Thesis]. University of Ottawa; 2016. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10393/34535.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ndikumana S. Genome Sequencing of the Relevant Zebrafish-Infecting Microsporidian Pseudoloma neurophilia Reveals Atypical Genome Dynamics . [Thesis]. University of Ottawa; 2016. Available from: http://hdl.handle.net/10393/34535

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Swedish University of Agricultural Sciences

15. Holmquist, Louise. Rhizoctonia solani and sugar beet responses.

Degree: 2018, Swedish University of Agricultural Sciences

URL: https://pub.epsilon.slu.se/15726/

► The soil-borne basidiomycete Rhizoctonia solani (strain AG2-2) incite root rot disease in sugar beet (Beta vulgaris). The overall objective of this thesis work was to… (more)

▼ The soil-borne basidiomycete Rhizoctonia solani (strain AG2-2) incite root rot disease in sugar beet (Beta vulgaris). The overall objective of this thesis work was to enhance the genomic knowledge on this pathogen and induced responses in the host to promote breeding of better performing cultivars. The AG2-2IIIB R. solani isolate sequenced in this project had a predicted genome size of 56.02 Mb and encoded 11,897 genes. In comparisons with four other R. solani genomes, the AG2-2IIIB genome contained more carbohydrate active enzymes, especially the polysaccharide lyase group represented by the pectate lyase family 1 (PL-1). When predicting for small, cysteine rich and secreted-proteins (effectors) 11 potential candidates were found to be AG2-2IIIB strain specific. In parallel, transcript data was generated from sugar beet breeding lines known to express differential responses to R. solani infection. After extensive data mining of the achieved information a handful of genes with potential roles in sugar beet defence were identified. Particularly three Bet v I/Major latex protein (MLP) homologous genes caught the interest and were further investigated together with three R. solani (Rs) effector candidates selected based on their transcript profiles during infection of sugar beet seedlings. They are: a rare lipoprotein-A like protein (RsRlpA), the chitin-binding lysin motif effector (RsLysM) and a cysteine-rich protein (RsCRP1). The three fungal effectors were induced upon early infection and were heterologously expressed in Cercospora beticola, a sugar beet leaf spot fungus, facilitating functional analysis. RsLysM showed perturbation of chitin-triggered plant immunity as expected but did not protect fungal hyphae from degradation. RsRlpA is localized to the plant plasma membrane and has capacity to suppress the hypersensitive response. When monitoring cellular localization of RsCRP1 it was found to target both plant mitochondria and chloroplasts. RsCRP1 was also used in pull-down experiments followed by amino acid sequencing from which a potential interacting protein, a plasma membrane intrinsic protein, BvPIP1;1 was proposed to be a candidate. The studies on the fungal effectors and the potential plant defence candidates involving BvMLPs and BvPIP1;1 are on-going including assays of gene homologs in Arabidopsis to promote mechanistic understanding of the sugar beet – R. solani interactions together with protein-protein interactions and associated assays. Results to be implemented in resistance breeding.

Subjects/Keywords: Rhizoctonia solani; sugar beet; resistance; RNAseq; Cercospora beticola; effectors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Holmquist, L. (2018). Rhizoctonia solani and sugar beet responses. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from https://pub.epsilon.slu.se/15726/

Chicago Manual of Style (16^th Edition):

Holmquist, Louise. “Rhizoctonia solani and sugar beet responses.” 2018. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed December 09, 2019. https://pub.epsilon.slu.se/15726/.

MLA Handbook (7^th Edition):

Holmquist, Louise. “Rhizoctonia solani and sugar beet responses.” 2018. Web. 09 Dec 2019.

Vancouver:

Holmquist L. Rhizoctonia solani and sugar beet responses. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2018. [cited 2019 Dec 09]. Available from: https://pub.epsilon.slu.se/15726/.

Council of Science Editors:

Holmquist L. Rhizoctonia solani and sugar beet responses. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2018. Available from: https://pub.epsilon.slu.se/15726/

16. Paula de Souza Cabral Costa. ExpressÃo de genes em Phytophthora nicothanae que codificam proteÃnas indutoras de respostas de defesa em plantas.

Degree: 2007, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=952

►

COSTA, Paula de Souza Cabral. ExpressÃo de genes em Phytophthora nicotianae que codificam proteÃnas indutoras de respostas de defesa em plantas. 2007. 56p. Tese (Doutorado… (more)

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COSTA, Paula de Souza Cabral. ExpressÃo de genes em Phytophthora nicotianae que codificam proteÃnas indutoras de respostas de defesa em plantas. 2007. 56p. Tese (Doutorado em Fisiologia Vegetal) â Universidade Federal de Lavras, Lavras, MG*. O estudo da interaÃÃo citros-Phytophthora nicotianae foi focalizado dentro do projeto MilÃnio de Citros, tendo sido gerado um banco de ESTs de Phytophthora nicotianae isolado IAC 095 (PP/CitEST) apÃs induÃÃo in planta e subcultivo in vitro por 40 vezes. Os objetivos deste trabalho foram adequar metodologia para cultivo e separaÃÃo de fases do ciclo de vida de Phytophthora nicotianae de citros e analisar a expressÃo de seus genes que codificam proteÃnas extracelulares indutoras de respostas de defesa em plantas, usando PCR quantitativo (qRT-PCR). Frutos de laranja foram inoculados com discos contendo micÃlio de P. nicotianae. ApÃs a infecÃÃo, o patÃgeno foi purificado em meio de cultura contendo antibiÃticos e 4 fases do seu ciclo de vida foram obtidas: ZoÃsporos, Hifa, Hifa com os esporÃngios cheios e Hifa com os esporÃngios vazios. cDNA foi sintetizado a partir de mRNA obtido das fases e a expressÃo gÃnica foi analisada por meio de qRT-PCR. A condiÃÃo adequada para o cultivo de P. nicotianae de citros in vitro, visando Ã separaÃÃo de fases do seu ciclo de vida, Ã em meio de cenoura lÃquido distribuÃdo em erlenmeyers, a 25Â C sob luz constante. Para cinco dos oito genes estudados (CBEL, Ric1, Bip, Car e Transglutaminase) o perfil de expressÃo foi similar, apresentando maior expressÃo nas fases de hifa e hifa com esporÃngios vazios. A proteÃna Megaspermina foi mais expressa em zoÃsporos do que em hifa, esporÃngios cheios e vazios. Por outro lado, as proteÃnas 14-3-3 e Sintaxina foram mais expressos em hifa do que nas outras fases estudadas. * ComitÃ Orientador: Prof. Dr. Luciano Vilela Paiva â UFLA (Orientador), DrÂ MagnÃlia de AraÃjo Campos â UFLA.

COSTA, Paula de Souza Cabral. Phytophthora nicotianae genes expression that encode induced-protein response defense in plants. 2007. 56p. Thesis (Doctor Science in Plant Physiology) â Federal University of Lavras, Lavras, MG*. The study of the Citros-Phytophthora nicotianae interaction was focused into the Citros Milenium project, and it has generated an ESTs database of Phytophthora nicotianae IAC 095 strain (PP/CitEST) after in planta induction and in vitro subculture for 40 times. The objectives of this study were to adequate the methodology to growth and citros Phytophthora nicotianae separation of life cycle phases and analyze its genes expression that codify extracellular proteins inductors of defense response in plants, using a quantitative PCR (RT-PCR). Orange fruits were inoculated with discs containing P. nicotianae mycelia. After infection, the pathogen was purified during the culture containing antibiotics and 4 phases of its life cycle it was obtained: Zoospores, mycelia, mycelia with empty sporangium. cDNA was synthesized from the mRNA obtained from the phases and the gene expression was analyzed by the qRT-PCR. The…

Advisors/Committee Members: MÃrio LÃcio Vilela de Resende, Alessandra Alves de Souza, MagnÃlia de AraÃjo Campos, Luciano Vilela Paiva.

Subjects/Keywords: citros; effectors; efetores; PCR quantitativo; FISIOLOGIA DE PLANTAS CULTIVADAS; citros; quantitative PCR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Costa, P. d. S. C. (2007). ExpressÃo de genes em Phytophthora nicothanae que codificam proteÃnas indutoras de respostas de defesa em plantas. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Costa, Paula de Souza Cabral. “ExpressÃo de genes em Phytophthora nicothanae que codificam proteÃnas indutoras de respostas de defesa em plantas.” 2007. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed December 09, 2019. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Costa, Paula de Souza Cabral. “ExpressÃo de genes em Phytophthora nicothanae que codificam proteÃnas indutoras de respostas de defesa em plantas.” 2007. Web. 09 Dec 2019.

Vancouver:

Costa PdSC. ExpressÃo de genes em Phytophthora nicothanae que codificam proteÃnas indutoras de respostas de defesa em plantas. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2007. [cited 2019 Dec 09]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Costa PdSC. ExpressÃo de genes em Phytophthora nicothanae que codificam proteÃnas indutoras de respostas de defesa em plantas. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2007. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

17. Michelle Bayerl Fernandes. Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro.

Degree: 2011, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3056

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The coffee leaf rust caused by the biotrophic fungus Hemileia vastatrix, it is the most important fungal disease of coffee, causing productivity losses and its… (more)

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The coffee leaf rust caused by the biotrophic fungus Hemileia vastatrix, it is the most important fungal disease of coffee, causing productivity losses and its control increases the costs of coffee production. This study aimed to identify genes of H. vastatrix that encode secreted proteins that may function as effectors needed for the establishment of the biotrophic interaction and / or as triggers of resistance responses, by analyzing a database consisting of 9828 expressed sequence tags (ESTs) from an susceptible interaction. These ESTs were generated by sequencing the 5 end of cDNA clones from a library constructed from mRNA isolated from coffee leaves collected 12 days after inoculation with single pustule isolation HV-01. It was obtained 1004 contigs and 3301 singlets after clustering the sequences using the CAP3 program. From 890 unique transcripts that encode proteins without similarity to sequences of non-redundant database of GenBank / NCBI, which had no identity to sequences of Coffea spp., also deposited in this database, were identified 46 ORFs that encode peptides over 60 amino acids with positive prediction from five or more parameters of algorithms used to predict secretion signal sequences. We selected five genes which were amplified from the H. vastatrix genome, generating fragments equal or higher than those amplified from cDNAs. These genes, unique to H. vastatrix showed no identity with cDNA sequences derived from germination spores, demonstrating that their expression may occur within the infected tissue. The secretion of proteins encoded by four genes was demonstrated using the Yeast Secretion System. Functional studies should be conducted to confirm the effector activity of the genes characterized, as well as from other genes identified in this study, whose origin and secretion of fungal the predicted proteins in yeast has been demonstrated.

A ferrugem causada pelo fungo biotrófico Hemileia vastatrix é a doença mais importante do cafeeiro, pois atinge, com gravidade, grandes áreas de lavouras, onde causa prejuízos na produtividade e seu controle aumenta os custos de produção. O presente trabalho teve por objetivo identificar genes de H. vastatrix que codificam proteínas secretadas que possam funcionar como efetores necessários para o estabelecimento da interação biotrófica e, ou, como desencadeadores de respostas de resistência, por meio da análise de um banco constituídos por 9828 etiquetas de sequências expressas (ESTs) em uma interação suscetível. Essas ESTs foram geradas pelo sequenciamento da extremidade 5de clones de cDNA de uma biblioteca construída a partir de mRNA isolado de folhas de cafeeiro coletadas 12 dias após a inoculação com o isolado monopustular HV-01. Foram obtidos 1004 contíguos e 3301 singletos após o alinhamento das sequências pelo programa CAP3. A partir de 890 transcritos únicos que codificam proteínas sem similaridade a sequências do banco não redundante do GenBank/NCBI e que não possuíam identidade a sequências de Coffea spp. também depositadas nesse banco de…

Advisors/Committee Members: Olinto Liparini Pereira, Eduardo Seiti Gomide Mizubuti, Marisa Vieira de Queiroz, Gleiber Quintão Furtado, Sérgio Hermínio Brommonschenkel.

Subjects/Keywords: Efetores; Hemileia vastatrix; Ferrugem do cafeeiro; FITOPATOLOGIA; Coffee rust; Hemileia vastatrix; Effectors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fernandes, M. B. (2011). Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3056

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernandes, Michelle Bayerl. “Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro.” 2011. Thesis, Universidade Federal de Viçosa. Accessed December 09, 2019. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3056.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernandes, Michelle Bayerl. “Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro.” 2011. Web. 09 Dec 2019.

Vancouver:

Fernandes MB. Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro. [Internet] [Thesis]. Universidade Federal de Viçosa; 2011. [cited 2019 Dec 09]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3056.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernandes MB. Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro. [Thesis]. Universidade Federal de Viçosa; 2011. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3056

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

18. Valeria Yukari Abe. Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência.

Degree: 2012, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4182

► Brazil is the second world soybean producer. Currently, the main limiting factor in this crop production is the Asian soybean rust (ASR) whose etiologic agent… (more)

▼ Brazil is the second world soybean producer. Currently, the main limiting factor in this crop production is the Asian soybean rust (ASR) whose etiologic agent is the fungus Phakopsora pachyrhizi. The rust fungi are obligate parasites that during their interaction with the plant, they secrete effector proteins that manipulate host metabolism and interfere with their defense responses. Some of these effector proteins, called Avr proteins, are recognized by encoded proteins by resistant R genes, which usually trigger a hypersensitivity response (HR) and resistance phenotype. At least, there are five described R genes (Rpp1 to Rpp5) that confer resistance to ASR and several genes that encode secreted proteins by this fungus were recently identified. However, the effector proteins (Avr) recognized by encoded proteins by Rpp genes were not identified yet. Since there is not a transformation assay protocol for P. pachyrhizi, a strategy to identify this fungus Avr proteins is the transient expression of R proteins in resistant varieties cytoplasm and the observation of a possible HR response. Thus, the specific objectives of this work were: to try to establish a methodology for transient expression in soybean by agroinfiltration using the gene GUSPlus as reporter gene; to establish a protocol for translocation of effector proteins by the type III secretion system (SST3) of Pseudomonas syringae pv. glycinea race 4 (Psg4), and also to evaluate the effector activity of candidate genes in soybean resistant genotypes to the isolate PPUFV02 of P. pachyrhizi. There was no expression of the gene GUSPlus in Agrobacterium tumefaciens strain EHA105 infiltrated soybean leaves, while using the same inoculum preparation and concentration of bacterial cells, there was a consistent expression of the gene GUSPlus in tobacco. This result derailed the use of agroinfiltration in the functional study of candidate genes in soybean effectors. All soybean genotypes evaluated were susceptible to Psg4, demonstrating that the viability to use this bacterial on functional analysis of candidate effector proteins mediated by SST3. Better symptoms reproducibility was observed with inoculation by vacuum infiltration of Psg4 in a bacterial concentration of OD600 = 0,01, for allowing a gradually symptoms analysis. The encoded protein by avrB gene is recognized by the RPG1 gene product, which is present in some genotypes of soybean. The construction pVSP61-avrB was able to induce HR in the genotype Williams 82, that contains the corresponding gene RPG1 and to induce it in the genotypes Conquista and PI 459025. This result allowed the use of this construction as a positive control for functional analysis of P. pachyrhizi putative effector proteins. Because of this, 12 sequences were cloned into vector pEDV6. This vector allows the expression of proteins of interest fused to secretion signal sequences by SST3, aiming its subsequent translocation into the cytosol. Nine from the constructions with pEDV6-PHPA_RSP transformed into Psg4 were submitted to functional… Advisors/Committee Members: Marisa Vieira de Queiroz, Sérgio Hermínio Brommonschenkel, Gleiber Quintão Furtado.

Subjects/Keywords: SST3; FITOPATOLOGIA; Efetores; Ferrugem asiática da soja; Agroinfiltração; Effectors; Asian soybean rust; SST3; Agroinfiltration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abe, V. Y. (2012). Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abe, Valeria Yukari. “Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência.” 2012. Thesis, Universidade Federal de Viçosa. Accessed December 09, 2019. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abe, Valeria Yukari. “Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência.” 2012. Web. 09 Dec 2019.

Vancouver:

Abe VY. Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência. [Internet] [Thesis]. Universidade Federal de Viçosa; 2012. [cited 2019 Dec 09]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abe VY. Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência. [Thesis]. Universidade Federal de Viçosa; 2012. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

19. Herrera Estrada, Lina Paola. Production and Engineering of Therapeutic Protein Nanoparticles.

Degree: PhD, Chemical and Biomolecular Engineering, 2014, Georgia Tech

URL: http://hdl.handle.net/1853/56153

► Protein nanoparticles were proposed as therapeutic protein or enzyme delivery vehicles. The production of protein-enzyme nanoparticles via desolvation and self-assembly was investigated and optimized. First,… (more)

▼ Protein nanoparticles were proposed as therapeutic protein or enzyme delivery vehicles. The production of protein-enzyme nanoparticles via desolvation and self-assembly was investigated and optimized. First, β-galactosidase –a model enzyme– was incorporated into enhanced green fluorescent protein (eGFP) nanoparticles prepared via desolvation. Particle size was shown to be sensitive to type of cross-linker, cross-linking time, and the presence of imidazole. Protein-enzyme nanoparticles are shown to effectively deliver active enzyme to multiple cell lines in vitro. Then the potential of protein nanoparticles for therapeutic protein and enzyme delivery was studied in two diseases models: inflammatory bowel disease (IBD) and breast cancer. Bacterial effector proteins, AvrA and YopJ, were proposed as potential therapeutic agents because of their ability to efficiently subvert the MAPK and NF-κB pathways, which have been implicated in the pathogenesis and progression of IBD and breast cancer. AvrA was incorporated into eGFP nanoparticles and the resulting particles were shown to effectively deliver the effector to target cells in vitro and in vivo, inhibit inflammatory signaling and decrease inflammation in murine colitis models. YopJ was fused to Glutathione-S-Transferase (GST), which caused self-assembly of the fusion into stable nanoparticles. These particles were shown to induce cell death in a panel of breast cancer cell lines, but were not cytotoxic to non-breast cancer cells. Furthermore, these particles decreased cell migration in vitro and performed better or as well as a chemotherapeutic agent, doxorubicin. This data suggests that protein nanoparticles are a biocompatible, high efficiency alternative for intracellular delivery of active enzyme therapeutics, and demonstrates the potential of effector proteins as therapeutic agents. Advisors/Committee Members: Champion, Julie A (advisor), Kemp, Melissa (committee member), Bommarius, Andreas (committee member), Payne, Christine (committee member), Taite, Lakeisha (committee member).

Subjects/Keywords: Nanoparticles; Enzymes; Bacterial Protein Effectors; Desolvation; Self-assembly; IBD; Breast Cancer; YopJ; AvrA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Herrera Estrada, L. P. (2014). Production and Engineering of Therapeutic Protein Nanoparticles. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/56153

Chicago Manual of Style (16^th Edition):

Herrera Estrada, Lina Paola. “Production and Engineering of Therapeutic Protein Nanoparticles.” 2014. Doctoral Dissertation, Georgia Tech. Accessed December 09, 2019. http://hdl.handle.net/1853/56153.

MLA Handbook (7^th Edition):

Herrera Estrada, Lina Paola. “Production and Engineering of Therapeutic Protein Nanoparticles.” 2014. Web. 09 Dec 2019.

Vancouver:

Herrera Estrada LP. Production and Engineering of Therapeutic Protein Nanoparticles. [Internet] [Doctoral dissertation]. Georgia Tech; 2014. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/1853/56153.

Council of Science Editors:

Herrera Estrada LP. Production and Engineering of Therapeutic Protein Nanoparticles. [Doctoral Dissertation]. Georgia Tech; 2014. Available from: http://hdl.handle.net/1853/56153

20. Truong, Nhat My. Analyse fonctionnelle des effecteurs nucléaires du parasitisme des nématodes à galle Meloidogyne incognita et caractérisation de leurs cibles végétales : Functional analysis of root knot nematode Meloidogyne incognita nuclear effectors and characterization of their plant targets.

Degree: Docteur es, Biologie moléculaire et cellulaire, 2016, Côte d'Azur

URL: http://www.theses.fr/2016AZUR4149

Le nématode à galle, Meloidogyne incognita est un parasite extrêmement polyphage capable d'induire la redifférentiation de cellules racinaires en cellules en cellules nourricières hypertrophiées.

The root-knot nematode Meloidogyne incognita is among the most devastating plant pathogens.

Advisors/Committee Members: Abad, Pierre (thesis director), Quentin, Michaël (thesis director).

Subjects/Keywords: Nématodes; Effecteurs; Plantes; Interaction; Double hybride; Nematodes; Effectors; Plants; Interaction; Yeast two hybrid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Truong, N. M. (2016). Analyse fonctionnelle des effecteurs nucléaires du parasitisme des nématodes à galle Meloidogyne incognita et caractérisation de leurs cibles végétales : Functional analysis of root knot nematode Meloidogyne incognita nuclear effectors and characterization of their plant targets. (Doctoral Dissertation). Côte d'Azur. Retrieved from http://www.theses.fr/2016AZUR4149

Chicago Manual of Style (16^th Edition):

Truong, Nhat My. “Analyse fonctionnelle des effecteurs nucléaires du parasitisme des nématodes à galle Meloidogyne incognita et caractérisation de leurs cibles végétales : Functional analysis of root knot nematode Meloidogyne incognita nuclear effectors and characterization of their plant targets.” 2016. Doctoral Dissertation, Côte d'Azur. Accessed December 09, 2019. http://www.theses.fr/2016AZUR4149.

MLA Handbook (7^th Edition):

Truong, Nhat My. “Analyse fonctionnelle des effecteurs nucléaires du parasitisme des nématodes à galle Meloidogyne incognita et caractérisation de leurs cibles végétales : Functional analysis of root knot nematode Meloidogyne incognita nuclear effectors and characterization of their plant targets.” 2016. Web. 09 Dec 2019.

Vancouver:

Truong NM. Analyse fonctionnelle des effecteurs nucléaires du parasitisme des nématodes à galle Meloidogyne incognita et caractérisation de leurs cibles végétales : Functional analysis of root knot nematode Meloidogyne incognita nuclear effectors and characterization of their plant targets. [Internet] [Doctoral dissertation]. Côte d'Azur; 2016. [cited 2019 Dec 09]. Available from: http://www.theses.fr/2016AZUR4149.

Council of Science Editors:

Truong NM. Analyse fonctionnelle des effecteurs nucléaires du parasitisme des nématodes à galle Meloidogyne incognita et caractérisation de leurs cibles végétales : Functional analysis of root knot nematode Meloidogyne incognita nuclear effectors and characterization of their plant targets. [Doctoral Dissertation]. Côte d'Azur; 2016. Available from: http://www.theses.fr/2016AZUR4149

21. Boulain, Hélène. Diversité, caractéristiques évolutives et rôles des effecteurs salivaires du puceron du pois dans l’interaction avec ses plantes hôtes : Diversity, evolutionary characteristics and role of pea aphid salivary effectors in the interaction with host plants.

Degree: Docteur es, Écologie et évolution, 2017, Rennes, Agrocampus Ouest

URL: http://www.theses.fr/2017NSARA082

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Les effecteurs jouent un rôle fondamental lors des interactions antagonistes plantes-pathogènes en supprimant les défenses de la plante, permettant ainsi aux parasites de se développer.… (more)

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Les effecteurs jouent un rôle fondamental lors des interactions antagonistes plantes-pathogènes en supprimant les défenses de la plante, permettant ainsi aux parasites de se développer. De tels effecteurs ont été caractérisés chez les insectes herbivores mais leur rôle dans la spécialisation à la plante reste méconnu. Les pucerons se nourrissent de la sève du phloème et injectent dans la plante des effecteurs salivaires. L'étude des patrons d’évolution des effecteurs, ainsi que la caractérisation de leurs fonctions sont nécessaires à la compréhension des mécanismes de spécialisation chez les pucerons. Au cours de ces travaux, nous avons cherché à identifier les effecteurs salivaires impliqués dans l'adaptation du puceron du pois, Acyrthosiphon pisum, à ses hôtes.Des approches évolutives, basées sur un nouveau catalogue de 740 effecteurs candidats surexprimés dans les glandes salivaires de A. pisum, ont révélé que certains d'entre eux évoluent rapidement et que l'expansion de familles multigéniques apparaît comme une source importante de diversité des effecteurs. En parallèle, ces travaux ont permis d'optimiser l'expression transitoire médiée par Agrobacterium dans le pois. Ce nouvel outil d'analyse fonctionnelle permet maintenant l'étude des effecteurs candidats afin d'identifier les effecteurs du puceron du pois impliqués dans l'adaptation à la plante hôte.

Effectors play fundamental roles in antagonistic plant-pathogen interactions mainly by suppressing plant defense and allow parasites to multiply on the plant. Some effectors have been characterized in herbivorous insects; however, their role to the evolution in plant specialization remains unknown. Aphids feed from phloem sap and inject salivary effectors into the host plant. Studying evolutionary patterns and characterizing functions of effectors appear as important steps toward unveiling the mechanisms of host plant specialization in aphids. This work sought to identify salivary effectors that are involved in plant specialization of the pea aphid, Acyrthosiphon pisum. Evolutionary approaches based on a new catalogue of 740 putative effectors that are up-regulated in salivary glands of A. pisum revealed that some of them evolve rapidly.Moreover, gene family expansion appear as an important source of novel effectors. In parallel, this work optimized Agrobacterium-mediated transient gene expression in pea to provide a new tool for functional analyses of pea aphid effectors. The construction of a comprehensive catalogue of A. pisum salivary effectors and evolutionary analysis of them provide new candidates in host plant adaptation. By using the gene expression tool now available in pea, functional characterization of candidates will help to identify the effectors that are involved in plant specialization of the pea aphid.

Advisors/Committee Members: Simon, Jean-Christophe (thesis director), Sugio, Akiko (thesis director).

Subjects/Keywords: Effecteurs salivaires; Puceron du pois; Interactions plantes-Pucerons; Salivary effectors; Pea aphid; Plant-Aphid interactions

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boulain, H. (2017). Diversité, caractéristiques évolutives et rôles des effecteurs salivaires du puceron du pois dans l’interaction avec ses plantes hôtes : Diversity, evolutionary characteristics and role of pea aphid salivary effectors in the interaction with host plants. (Doctoral Dissertation). Rennes, Agrocampus Ouest. Retrieved from http://www.theses.fr/2017NSARA082

Chicago Manual of Style (16^th Edition):

Boulain, Hélène. “Diversité, caractéristiques évolutives et rôles des effecteurs salivaires du puceron du pois dans l’interaction avec ses plantes hôtes : Diversity, evolutionary characteristics and role of pea aphid salivary effectors in the interaction with host plants.” 2017. Doctoral Dissertation, Rennes, Agrocampus Ouest. Accessed December 09, 2019. http://www.theses.fr/2017NSARA082.

MLA Handbook (7^th Edition):

Boulain, Hélène. “Diversité, caractéristiques évolutives et rôles des effecteurs salivaires du puceron du pois dans l’interaction avec ses plantes hôtes : Diversity, evolutionary characteristics and role of pea aphid salivary effectors in the interaction with host plants.” 2017. Web. 09 Dec 2019.

Vancouver:

Boulain H. Diversité, caractéristiques évolutives et rôles des effecteurs salivaires du puceron du pois dans l’interaction avec ses plantes hôtes : Diversity, evolutionary characteristics and role of pea aphid salivary effectors in the interaction with host plants. [Internet] [Doctoral dissertation]. Rennes, Agrocampus Ouest; 2017. [cited 2019 Dec 09]. Available from: http://www.theses.fr/2017NSARA082.

Council of Science Editors:

Boulain H. Diversité, caractéristiques évolutives et rôles des effecteurs salivaires du puceron du pois dans l’interaction avec ses plantes hôtes : Diversity, evolutionary characteristics and role of pea aphid salivary effectors in the interaction with host plants. [Doctoral Dissertation]. Rennes, Agrocampus Ouest; 2017. Available from: http://www.theses.fr/2017NSARA082

UCLA

22. Nguyen, HoangKim Tran. Understanding Mechanisms of Motility in Trypanosoma brucei.

Degree: Microbiology, Immunology, & Molecular Genetics, 2014, UCLA

URL: http://www.escholarship.org/uc/item/7md8b9j5

► Trypanosoma brucei are protozoan parasites that present a tremendous medical and economic burden on poverty-stricken areas of sub-Saharan Africa. These pathogens are estimated to affect… (more)

▼ Trypanosoma brucei are protozoan parasites that present a tremendous medical and economic burden on poverty-stricken areas of sub-Saharan Africa. These pathogens are estimated to affect nearly 60 million people and are considered one the world's most neglected diseases. A key aspect for parasite pathogenicity and transmission is the mechanism of motility. Recent advances have revealed that in addition to the ability to swim in incredibly viscous environments, T. brucei parasites are capable of forming multicellular communities upon surface exposure. Although this behavior is ubiquitous in microbiology, this is the first described pathogenic protozoa capable of coordinating motility for polarized movement as a multicellular group. Through both forward and reverse genetic screens, this study outlines efforts to define signaling pathways and genes required for this surface-induced behavior. In particular, cAMP has been implicated in this process and a biased screen was performed to identify cAMP effectors that are required for social motility. At individual level, motility in T. brucei is achieved by a single flagellum that is attached along the length of the cell body. The T. brucei flagellum has a canonical 9 + 2 axoneme structure that is highly conserved in organisms with motile flagella. A previous phylogenetic study identified 50 genes as the Core components of Motile Flagella (CMF). This dissertation expanded the original study and compared 115 diverse eukaryotic genomes. CMF22 was identified as a broadly conserved gene that was present in almost all organisms with motile flagella but not in organisms that lack flagella or have immotile flagella. Biochemical fractionation, localization by epitope tagging and RNAi knockdown was used to characterize CMF22. These experiments have demonstrated that CMF22 is an axonemal protein required for propulsive motility. High-resolution cryo-electron tomography of CMF22 knockdown mutants revealed the requirement of CMF22 for assembly of the proximal lobe of the nexin-dynein regulatory complex (N-DRC). Finally, mutagenesis experiments of the putative IQ motif and AAA domain of CMF22 have elucidated a potential mechanism for CMF22 action in axonemal motility. Altogether, this work reveals important findings of motility, both at the individual and multicellular level to enhance current understand of protozoan biology, social microbiology, and conserved mechanisms of flagellum motility.

Subjects/Keywords: Microbiology; axonemal motility; cNMP effectors; flagellum; nexin-dynein regulatory complex; social motility; Trypanosoma brucei

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, H. T. (2014). Understanding Mechanisms of Motility in Trypanosoma brucei. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/7md8b9j5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nguyen, HoangKim Tran. “Understanding Mechanisms of Motility in Trypanosoma brucei.” 2014. Thesis, UCLA. Accessed December 09, 2019. http://www.escholarship.org/uc/item/7md8b9j5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nguyen, HoangKim Tran. “Understanding Mechanisms of Motility in Trypanosoma brucei.” 2014. Web. 09 Dec 2019.

Vancouver:

Nguyen HT. Understanding Mechanisms of Motility in Trypanosoma brucei. [Internet] [Thesis]. UCLA; 2014. [cited 2019 Dec 09]. Available from: http://www.escholarship.org/uc/item/7md8b9j5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nguyen HT. Understanding Mechanisms of Motility in Trypanosoma brucei. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/7md8b9j5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

23. Osman, Rahwa A 1982-. THE ROLE OF INTERFERON AND NON-CONVENTIONAL T-CELLS IN THE CLEARANCE OF PRIMARY BHV-1 RESPIRATORY INFECTION.

Degree: 2018, University of Saskatchewan

URL: http://hdl.handle.net/10388/8485

► Bovine herpesvirus-1 (BHV-1) is prevalent globally and is an important cause of bovine disease. Humoral and cell-mediated immune responses contribute to viral clearance following a… (more)

▼ Bovine herpesvirus-1 (BHV-1) is prevalent globally and is an important cause of bovine disease. Humoral and cell-mediated immune responses contribute to viral clearance following a primary BHV-1 respiratory infection but innate immune mechanisms contributing to early control of BHV-1 infection are not known. Gene expression analyses and immunohistochemical (IHC) studies were used to investigate the role of interferons (IFNs) and innate immune cells during a primary BHV-1 infection. There was significant (P < 0.05) induction of type I IFN and IFN-stimulated genes (ISGs) in the URT within 3 days post-infection (pi) but virus shedding continued for another 4 to 7 days. In vitro studies demonstrated that both type I and II IFNs had limited capacity to inhibit BHV-1 replication and BHV-1 infection did not block ISG transcription. Consequently, immune cell recruitment to the URT was analyzed following BHV-1 infection to determine if alternative defence mechanisms were activated. Morphometric analyses of lymphocytes in URT tissues before and after BHV-1 infection revealed significant (P < 0.05) increases in cell populations expressing CD335+ (NKp46 natural cytotoxicity receptor), CD3+ (T-cell lineage marker) and CD8+ (cytotoxic T-cell marker) on day 5 pi. Confocal microscopy confirmed that approximately 90% of lymphocytes present on day 5 pi were T-cells co-expressing CD335 and CD8. This bovine T-cell subpopulation is known as non-conventional T cells, which may be innate lymphocytes. Specific recruitment of non-conventional T-cells to the site of BHV-1 infection in the URT raised questions regarding what chemokines may be involved in regulating this selective cell migration. Chemokine(s) involved in non-conventional T-cell recruitment in cattle are currently not known. qRT-PCR analysis of known bovine chemokine genes revealed that the expression of CCL4, CCL5 and CXCL9 genes were significantly (P < 0.05) up-regulated following BHV-1 infection in nasal turbinates. Thus, one or more of these chemokines may be involved in the selective recruitment of bovine non-conventional T-cells to the site of BHV-1 infection. The specific recruitment of non-conventional T cells to infected tissue suggests these cells may play an important role in either viral clearance or regulating host responses during BHV-1 infection. Advisors/Committee Members: Griebel, Philip J, Mutwiri, George, Tikoo, Suresh.

Subjects/Keywords: Interferon; interferon-induced antiviral effectors; Non-conventional T-cells; Bovine herpesvirus -1; lymphocyte recruitment; chemokines

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APA (6^th Edition):

Osman, R. A. 1. (2018). THE ROLE OF INTERFERON AND NON-CONVENTIONAL T-CELLS IN THE CLEARANCE OF PRIMARY BHV-1 RESPIRATORY INFECTION. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/8485

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Osman, Rahwa A 1982-. “THE ROLE OF INTERFERON AND NON-CONVENTIONAL T-CELLS IN THE CLEARANCE OF PRIMARY BHV-1 RESPIRATORY INFECTION.” 2018. Thesis, University of Saskatchewan. Accessed December 09, 2019. http://hdl.handle.net/10388/8485.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Osman, Rahwa A 1982-. “THE ROLE OF INTERFERON AND NON-CONVENTIONAL T-CELLS IN THE CLEARANCE OF PRIMARY BHV-1 RESPIRATORY INFECTION.” 2018. Web. 09 Dec 2019.

Vancouver:

Osman RA1. THE ROLE OF INTERFERON AND NON-CONVENTIONAL T-CELLS IN THE CLEARANCE OF PRIMARY BHV-1 RESPIRATORY INFECTION. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10388/8485.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Osman RA1. THE ROLE OF INTERFERON AND NON-CONVENTIONAL T-CELLS IN THE CLEARANCE OF PRIMARY BHV-1 RESPIRATORY INFECTION. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/8485

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

24. Asper, David Jose. Colonization of cattle by non-O157 Shiga Toxin-producing Escherichia coli serotypes.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-09292009-112751

► Shiga toxin-producing E. coli (STEC) is an important food- and water-borne pathogen of humans, causing Hemorrhagic Colitis and Haemolytic Uremic Syndrome. Colonization of both cattle… (more)

▼ Shiga toxin-producing E. coli (STEC) is an important food- and water-borne pathogen of humans, causing Hemorrhagic Colitis and Haemolytic Uremic Syndrome. Colonization of both cattle and human hosts is mediated through the action of effector molecules secreted via a type III secretion system (T3SS), which forms attaching and effacing lesions (A/E). The necessary effectors which form A/E by manipulation of host signalling and actin nucleation are present on a pathogenicity island called the Locus of Enterocyte Effacement (LEE). It has been reported that vaccination of cattle with Type III-secreted proteins (T3SPs) from STEC O157 resulted in decreased shedding. In order to extend this to non-O157 STEC serotypes, we examined the serological cross-reactivity of T3SPs of serotypes O26:H11, O103:H2, O111:NM and O157:H7. Groups of cattle were vaccinated with T3SPs produced from each of the serotypes and the magnitude and specificity of the responses were measured resulting in limited cross reactivity. Overall, results suggest that vaccination of cattle with T3SPs as a means of reducing the risk of STEC transmission to humans will induce protection that is serotype specific. To pursue the possibility of a cross-protective vaccine, we investigated the protective properties of a chimeric Tir protein against STEC serotypes. Several studies have reported that Tir is highly immunogenic and capable of producing high antibody titers. Potter and colleagues also demonstrated that the vaccination of cattle with ∆tir STEC O157 strain did not protect as well as the wildtype strain. We constructed thirty-mer peptides to the entire STEC O157 Tir protein, as well as to the intimin binding domain of the Tir protein from STEC serotype O26, O103 and O111. Using sera raised against STEC O157 and non-O157 T3SPs, we identified a number of immunogenic peptides containing epitopes unique to a particular serotype. Two different chimeric Tir proteins were constructed containing the STEC O157 Tir protein fused with six STEC non-O157 peptides with or without the Leukotoxin produced by Mannheimia haemolytica. However, the vaccination of mice with the chimeric protein did not protect against challenge with STEC O157 or STEC O111. These results suggest that to achieve cross protection against STEC serotypes using a recombinant protein vaccine, other immunogenic and protective antigens must also be included. In order to identify other immunogenic and cross-protective antigens we cloned and expressed the genes coding for 66 effectors and purified each as histidine-tagged proteins. These included 37 LEE-encoded proteins and 29 non-LEE effectors. The serological response against each protein was measured by Western blot analysis and an enzyme-linked immunosorbent assay (ELISA) using sera from rabbits immunized with T3SPs from four STEC serotypes, experimentally infected cattle and human sera from 6 HUS patients. A total of 20 proteins were recognized by at least one of the STEC T3SP- vaccinated rabbits using Western blots. Sera from… Advisors/Committee Members: Potter, Andrew.

Subjects/Keywords: Shiga Toxin-producing Escherichia coli; Type III Secretion System; effectors; non-O157 serotypes; vaccine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Asper, D. J. (2009). Colonization of cattle by non-O157 Shiga Toxin-producing Escherichia coli serotypes. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-09292009-112751

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Asper, David Jose. “Colonization of cattle by non-O157 Shiga Toxin-producing Escherichia coli serotypes.” 2009. Thesis, University of Saskatchewan. Accessed December 09, 2019. http://hdl.handle.net/10388/etd-09292009-112751.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Asper, David Jose. “Colonization of cattle by non-O157 Shiga Toxin-producing Escherichia coli serotypes.” 2009. Web. 09 Dec 2019.

Vancouver:

Asper DJ. Colonization of cattle by non-O157 Shiga Toxin-producing Escherichia coli serotypes. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10388/etd-09292009-112751.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Asper DJ. Colonization of cattle by non-O157 Shiga Toxin-producing Escherichia coli serotypes. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-09292009-112751

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Exeter

25. Lalik, Marta. Characterisation of secreted effector proteins of Nosema ceranae, an agent associated with Colony Collapse Disorder (CCD).

Degree: PhD, 2015, University of Exeter

URL: http://hdl.handle.net/10871/17647

► Nosema ceranae, a microsporidian, has been given much attention in recent years as it has been linked with Colony Collapse Disorder (CCD), which leads to… (more)

Subjects/Keywords: 638; Honey bees; colony collapse disorder (CCD); Nosema ceranae; nosemosis; effectors; secreted proteins; secretome; ORFeome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lalik, M. (2015). Characterisation of secreted effector proteins of Nosema ceranae, an agent associated with Colony Collapse Disorder (CCD). (Doctoral Dissertation). University of Exeter. Retrieved from http://hdl.handle.net/10871/17647

Chicago Manual of Style (16^th Edition):

Lalik, Marta. “Characterisation of secreted effector proteins of Nosema ceranae, an agent associated with Colony Collapse Disorder (CCD).” 2015. Doctoral Dissertation, University of Exeter. Accessed December 09, 2019. http://hdl.handle.net/10871/17647.

MLA Handbook (7^th Edition):

Lalik, Marta. “Characterisation of secreted effector proteins of Nosema ceranae, an agent associated with Colony Collapse Disorder (CCD).” 2015. Web. 09 Dec 2019.

Vancouver:

Lalik M. Characterisation of secreted effector proteins of Nosema ceranae, an agent associated with Colony Collapse Disorder (CCD). [Internet] [Doctoral dissertation]. University of Exeter; 2015. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10871/17647.

Council of Science Editors:

Lalik M. Characterisation of secreted effector proteins of Nosema ceranae, an agent associated with Colony Collapse Disorder (CCD). [Doctoral Dissertation]. University of Exeter; 2015. Available from: http://hdl.handle.net/10871/17647

26. Maeder, Morgan Lee. Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation.

Degree: PhD, Biology: Medical Sciences, Division of, 2013, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156806

► Engineered DNA-binding proteins enable targeted manipulation of the genome. Zinc fingers are the most well characterized DNA-binding domain and for many years research has focused… (more)

Subjects/Keywords: Genetics; Molecular biology; gene regulation; Genome Engineering; Transcription activator-like effectors; zinc finger nucleases

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maeder, M. L. (2013). Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156806

Chicago Manual of Style (16^th Edition):

Maeder, Morgan Lee. “Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation.” 2013. Doctoral Dissertation, Harvard University. Accessed December 09, 2019. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156806.

MLA Handbook (7^th Edition):

Maeder, Morgan Lee. “Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation.” 2013. Web. 09 Dec 2019.

Vancouver:

Maeder ML. Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation. [Internet] [Doctoral dissertation]. Harvard University; 2013. [cited 2019 Dec 09]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156806.

Council of Science Editors:

Maeder ML. Engineered DNA-Binding Proteins for Targeted Genome Editing and Gene Regulation. [Doctoral Dissertation]. Harvard University; 2013. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156806

Virginia Tech

27. Traore, Sy M. Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits.

Degree: PhD, Horticulture, 2014, Virginia Tech

URL: http://hdl.handle.net/10919/64519

► Bacterial fruit blotch (BFB) of cucurbits is caused by Acidovorax citrulli, a Gram-negative seedborne bacterium that can cause up to 100% fruit yield losses in… (more)

▼ Bacterial fruit blotch (BFB) of cucurbits is caused by Acidovorax citrulli, a Gram-negative seedborne bacterium that can cause up to 100% fruit yield losses in the field. Currently, BFB is a major problem for the cucurbits industry worldwide. Thus far, attempts to identify resistance in cucurbit germplasm for controlling BFB have been unsuccessful. Despite the importance of the disease, little is known about the molecular mechanisms of A. citrulli pathogenicity, due to a lack of molecular tools for studying the A. citrulli/cucurbit interaction. The genomic sequence of A. citrulli strain AAC00-1 has been determined, and the components of type III secretion system have been identified. The goal of this research was to develop molecular tools for studying the BFB disease. Nineteen putative type III effector genes were cloned from two representative A. citrulli strains (AAC00-1 and M6). The distribution of 19 type III effectors among A. citrulli strains, collected worldwide, was studied. A novel Gateway-compatible binary vector was developed for transient expression of A. citrulli type III effectors genes in planta. A set of modified vectors for marker-exchange mutagenesis in A. citrulli were constructed. The model plant species Nicotiana benthamiana was found to be susceptible to A. citrulli, while Nicotiana tabacum was resistance to A. citrulli, so therefore could carry nonhost resistance genes. Two T3S effectors, Aave1548 and Aave2166, triggered water soaking-like cell death in N. benthamiana, but HR-like cell death in N. tabacum. Bacterial mutagenesis and in planta disease assay confirmed that both Aave1548 and Aave2166 have significant virulence contributions to A. citrulli in N. benthamiana plant and melon seeds. Aave2166 encodes a putative acetyltransferase that belongs to the YopJ super family, which is conserved in both animal and plant pathogenic bacteria. Wild type but not the putative catalytic mutant (C232A) of Aave2166 can trigger cell death phenotype in N. benthamiana and N. tabacum. N. benthamiana yeast two-hybrid cDNA library screening using Aave2166 identified six N. benthamiana proteins/peptides which specifically interacted with Aave2166. Further characterization of these Aave2166 interactors may allow us to understand the virulence mechanism provided by Aave2166. The identification of nonhost resistance genes that can recognize Aave2166 and other type III effectors may help to develop novel strategies to control BFB disease of cucurbit. Advisors/Committee Members: Zhao, Bingyu (committeechair), Welbaum, Gregory E. (committee member), Beers, Eric P. (committee member), McDowell, John M. (committee member), Vinatzer, Boris A. (committee member).

Subjects/Keywords: A. citrulli; T3S effectors; marker-exchange mutagenesis; Nicotiana benthamiana; Nicotiana tabacum; plant immunity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Traore, S. M. (2014). Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/64519

Chicago Manual of Style (16^th Edition):

Traore, Sy M. “Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits.” 2014. Doctoral Dissertation, Virginia Tech. Accessed December 09, 2019. http://hdl.handle.net/10919/64519.

MLA Handbook (7^th Edition):

Traore, Sy M. “Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits.” 2014. Web. 09 Dec 2019.

Vancouver:

Traore SM. Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits. [Internet] [Doctoral dissertation]. Virginia Tech; 2014. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10919/64519.

Council of Science Editors:

Traore SM. Characterization of Effector Genes in Acidovorax citrulli the Causing Agent of Bacteria Fruit Blotch Disease of Cucurbits. [Doctoral Dissertation]. Virginia Tech; 2014. Available from: http://hdl.handle.net/10919/64519

Virginia Tech

28. Davis, Colin Lee. Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean.

Degree: PhD, Crop and Soil Environmental Sciences, 2017, Virginia Tech

URL: http://hdl.handle.net/10919/77857

► Estimated at approximately $43 billion annually, the cultivated soybean Glycine max (L.) Merr., is the second most valuable crop in the United States. Soybeans account… (more)

Subjects/Keywords: Phytophthora sojae; Soybean Mosaic Virus; effectors; Glycine max; Soybean; R-genes; gene mapping

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Davis, C. L. (2017). Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77857

Chicago Manual of Style (16^th Edition):

Davis, Colin Lee. “Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean.” 2017. Doctoral Dissertation, Virginia Tech. Accessed December 09, 2019. http://hdl.handle.net/10919/77857.

MLA Handbook (7^th Edition):

Davis, Colin Lee. “Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean.” 2017. Web. 09 Dec 2019.

Vancouver:

Davis CL. Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean. [Internet] [Doctoral dissertation]. Virginia Tech; 2017. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/10919/77857.

Council of Science Editors:

Davis CL. Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean. [Doctoral Dissertation]. Virginia Tech; 2017. Available from: http://hdl.handle.net/10919/77857

Cornell University

29. Elzinga, Dezi. Characterization Of Myzus Persicae (Green Peach Aphid) Salivary Effector Proteins And Their Function In Mediating Plant-Aphid Interactions .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/40616

Subjects/Keywords: Myzus persicae; salivary effectors; plant defense

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Elzinga, D. (2015). Characterization Of Myzus Persicae (Green Peach Aphid) Salivary Effector Proteins And Their Function In Mediating Plant-Aphid Interactions . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/40616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elzinga, Dezi. “Characterization Of Myzus Persicae (Green Peach Aphid) Salivary Effector Proteins And Their Function In Mediating Plant-Aphid Interactions .” 2015. Thesis, Cornell University. Accessed December 09, 2019. http://hdl.handle.net/1813/40616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elzinga, Dezi. “Characterization Of Myzus Persicae (Green Peach Aphid) Salivary Effector Proteins And Their Function In Mediating Plant-Aphid Interactions .” 2015. Web. 09 Dec 2019.

Vancouver:

Elzinga D. Characterization Of Myzus Persicae (Green Peach Aphid) Salivary Effector Proteins And Their Function In Mediating Plant-Aphid Interactions . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/1813/40616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elzinga D. Characterization Of Myzus Persicae (Green Peach Aphid) Salivary Effector Proteins And Their Function In Mediating Plant-Aphid Interactions . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Giraldo, Martha Cecilia. In planta characterization of Magnaporthe oryzae biotrophy-associated secreted (BAS) proteins and key secretion components.

Degree: PhD, Department of Plant Pathology, 2010, Kansas State University

URL: http://hdl.handle.net/2097/6761

► Rice blast caused by the ascomycetous fungus Magnaporthe oryzae remains a threat to global sustainable agriculture and food security. This pathogen infects staple cereal crops… (more)

▼ Rice blast caused by the ascomycetous fungus Magnaporthe oryzae remains a threat to global sustainable agriculture and food security. This pathogen infects staple cereal crops such as rice, wheat, barley and millets, as well as turf grasses, in a distinct way among fungal plant pathogens, which we described in the first chapter. In addition to economical importance, rice blast is a model pathosystem for difficult-to-study biotrophic fungi and fungal-plant interactions. We are studying proteins that fungi secrete inside living cells to block plant defenses and control host cell processes; these proteins are called effectors. To date mechanisms for secretion and delivery of effectors inside host cells during disease establishment remain unknown. This step is critical to ensure the successful infection. So far, the only commonality found among all unique small-secreted blast effector proteins is their accumulation in a novel in planta structure called the biotrophic-interfacial complex (BIC). Identifying effectors and understanding how they function inside rice cells are important for attaining durable disease control. In the second chapter, we presented one approach to address this challenge. We characterized four candidate effector genes that were highly expressed specifically during the rice cell invasion. Using transgenic fungi that secrete fluorescently-labeled versions of each protein allowed me to follow them during invasion in vivo by live cell imaging. These candidates show distinct secretion patterns suggesting a spatially-segregated secretion mechanism for effectors. Results revealed a BIC-located strong candidate cytoplasmic blast effector, two putative cell-to-cell movement proteins and a putative extrainvasive hyphal membrane (EIHM)-matrix protein, which has become a valuable tool for assessing successful infection sites. In the third chapter, we test if normal secretion components of filamentous fungi are involved in accumulation of effectors into BICs. We report localization studies with M. oryzae orthologs of conserved secretion machinery components to investigate secretion mechanisms for effectors showing preferential BIC accumulation and for non-BIC proteins such as BAS4. Especially bright fluorescence adjacent to BICs from Mlc1p (Myosin Light Chain, a Spitzenkörper marker), from Snc1p (a secretory vesicle marker), and from Yup1p (a putative t-SNARE endosomal protein) suggest secretion actively occurs in the BIC-associated cells. Localization of Spa2p (a polarisome marker), as a distinct spot at the tips of the bulbous invasive hyphae (IH) in planta, suggests the existence of two secretion complexes after the fungus switches growth from the polarized filamentous primary hyphae to bulbous IH. In the final chapter on future perspectives, we present some strategies towards the molecular understanding of the M. oryzae secretion mechanism during biotrophic invasion, which will lead to novel strategies for disease control. Advisors/Committee Members: Barbara S. Valent.

Subjects/Keywords: Magnaporthe oryzae; blast effectors; Biotrophy-associated secreted (BAS) proteins; Spitzenkorper; Polarisome; Mlc1p; Agriculture, Agronomy (0285)

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APA (6^th Edition):

Giraldo, M. C. (2010). In planta characterization of Magnaporthe oryzae biotrophy-associated secreted (BAS) proteins and key secretion components. (Doctoral Dissertation). Kansas State University. Retrieved from http://hdl.handle.net/2097/6761

Chicago Manual of Style (16^th Edition):

Giraldo, Martha Cecilia. “In planta characterization of Magnaporthe oryzae biotrophy-associated secreted (BAS) proteins and key secretion components.” 2010. Doctoral Dissertation, Kansas State University. Accessed December 09, 2019. http://hdl.handle.net/2097/6761.

MLA Handbook (7^th Edition):

Giraldo, Martha Cecilia. “In planta characterization of Magnaporthe oryzae biotrophy-associated secreted (BAS) proteins and key secretion components.” 2010. Web. 09 Dec 2019.

Vancouver:

Giraldo MC. In planta characterization of Magnaporthe oryzae biotrophy-associated secreted (BAS) proteins and key secretion components. [Internet] [Doctoral dissertation]. Kansas State University; 2010. [cited 2019 Dec 09]. Available from: http://hdl.handle.net/2097/6761.

Council of Science Editors:

Giraldo MC. In planta characterization of Magnaporthe oryzae biotrophy-associated secreted (BAS) proteins and key secretion components. [Doctoral Dissertation]. Kansas State University; 2010. Available from: http://hdl.handle.net/2097/6761

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