subject:(ES cells)

1. Nitta, Mai. Approaches to understanding the mechanism of mammalian folliculogenesis : Analysis of gap junctions in ovarian follicles, and the establishment of an in vitro system for obtaining follicles from mouse embryonic stem cells : 哺乳類卵胞形成機構の解析：卵胞発育におけるギャップ結合の解析とマウス胚性幹細胞からの卵胞誘導系の構築; ホニュウルイランホウケイセイキコウノカイセキ：ランホウハツイクニオケルギャップケツゴウノカイセキトマウスハイセイカンサイボウカラノランホウユウドウケイノコウチク.

Degree: Nara Institute of Science and Technology / 奈良先端科学技術大学院大学

URL: http://hdl.handle.net/10061/5726

Subjects/Keywords: ES cells

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APA (6^th Edition):

Nitta, M. (n.d.). Approaches to understanding the mechanism of mammalian folliculogenesis : Analysis of gap junctions in ovarian follicles, and the establishment of an in vitro system for obtaining follicles from mouse embryonic stem cells : 哺乳類卵胞形成機構の解析：卵胞発育におけるギャップ結合の解析とマウス胚性幹細胞からの卵胞誘導系の構築; ホニュウルイランホウケイセイキコウノカイセキ：ランホウハツイクニオケルギャップケツゴウノカイセキトマウスハイセイカンサイボウカラノランホウユウドウケイノコウチク. (Thesis). Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10061/5726

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nitta, Mai. “Approaches to understanding the mechanism of mammalian folliculogenesis : Analysis of gap junctions in ovarian follicles, and the establishment of an in vitro system for obtaining follicles from mouse embryonic stem cells : 哺乳類卵胞形成機構の解析：卵胞発育におけるギャップ結合の解析とマウス胚性幹細胞からの卵胞誘導系の構築; ホニュウルイランホウケイセイキコウノカイセキ：ランホウハツイクニオケルギャップケツゴウノカイセキトマウスハイセイカンサイボウカラノランホウユウドウケイノコウチク.” Thesis, Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Accessed March 04, 2021. http://hdl.handle.net/10061/5726.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nitta, Mai. “Approaches to understanding the mechanism of mammalian folliculogenesis : Analysis of gap junctions in ovarian follicles, and the establishment of an in vitro system for obtaining follicles from mouse embryonic stem cells : 哺乳類卵胞形成機構の解析：卵胞発育におけるギャップ結合の解析とマウス胚性幹細胞からの卵胞誘導系の構築; ホニュウルイランホウケイセイキコウノカイセキ：ランホウハツイクニオケルギャップケツゴウノカイセキトマウスハイセイカンサイボウカラノランホウユウドウケイノコウチク.” Web. 04 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Nitta M. Approaches to understanding the mechanism of mammalian folliculogenesis : Analysis of gap junctions in ovarian follicles, and the establishment of an in vitro system for obtaining follicles from mouse embryonic stem cells : 哺乳類卵胞形成機構の解析：卵胞発育におけるギャップ結合の解析とマウス胚性幹細胞からの卵胞誘導系の構築; ホニュウルイランホウケイセイキコウノカイセキ：ランホウハツイクニオケルギャップケツゴウノカイセキトマウスハイセイカンサイボウカラノランホウユウドウケイノコウチク. [Internet] [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; [cited 2021 Mar 04]. Available from: http://hdl.handle.net/10061/5726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Nitta M. Approaches to understanding the mechanism of mammalian folliculogenesis : Analysis of gap junctions in ovarian follicles, and the establishment of an in vitro system for obtaining follicles from mouse embryonic stem cells : 哺乳類卵胞形成機構の解析：卵胞発育におけるギャップ結合の解析とマウス胚性幹細胞からの卵胞誘導系の構築; ホニュウルイランホウケイセイキコウノカイセキ：ランホウハツイクニオケルギャップケツゴウノカイセキトマウスハイセイカンサイボウカラノランホウユウドウケイノコウチク. [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; Available from: http://hdl.handle.net/10061/5726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Edinburgh

2. Meek, Stephen Earl. Experimental approaches to establish rat embryonic stem cells.

Degree: PhD, 2011, University of Edinburgh

URL: http://hdl.handle.net/1842/5040

► The rat has been an established experimental animal model within many areas of biological investigation for over one hundred years due to its size, breeding… (more)

Subjects/Keywords: 571.6; ES cells; 2i; rat; Oct4; Nanog

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APA (6^th Edition):

Meek, S. E. (2011). Experimental approaches to establish rat embryonic stem cells. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5040

Chicago Manual of Style (16^th Edition):

Meek, Stephen Earl. “Experimental approaches to establish rat embryonic stem cells.” 2011. Doctoral Dissertation, University of Edinburgh. Accessed March 04, 2021. http://hdl.handle.net/1842/5040.

MLA Handbook (7^th Edition):

Meek, Stephen Earl. “Experimental approaches to establish rat embryonic stem cells.” 2011. Web. 04 Mar 2021.

Vancouver:

Meek SE. Experimental approaches to establish rat embryonic stem cells. [Internet] [Doctoral dissertation]. University of Edinburgh; 2011. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1842/5040.

Council of Science Editors:

Meek SE. Experimental approaches to establish rat embryonic stem cells. [Doctoral Dissertation]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/5040

University of Edinburgh

3. Wachter, Elisabeth. Influence of CpG islands on chromatin structure.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/9369

► CpG islands (CGIs) are short GC rich sequences with a high frequency of CpGs that are associated with the active chromatin mark H3K4me3. Most occur… (more)

▼ CpG islands (CGIs) are short GC rich sequences with a high frequency of CpGs that are associated with the active chromatin mark H3K4me3. Most occur at gene promoters and are often free of cytosine methylation. Recent work has begun to clarify the functional significance of CGIs with respect to chromatin structure and transcription. In particular, proteins associated with histone-modifying activities, such as Cfp1 and Kdm2a, bind specifically to non-methylated CGIs via their CxxC domains. For example, artificial promoterless CpG-rich sequences integrated at the 3’ UTR of genes recruit Cfp1 and generate novel peaks of H3K4me3 in mouse ES cells without apparent RNA polymerase recruitment. There is also evidence that G+C-rich DNA recruits H3K27me3, a gene silencing mark. In this thesis I am exploring the constraints on DNA sequence and genomic location that are required to impose both H3K4me3 and H3K27me3 at CGI sequences. Showing that the generation of novel peaks of H3K4me3 and H3K27me3 over a promoter-less CpG rich sequence in a gene desert region is independent of it’s location in the genome extends earlier findings. These findings suggest that shared features of the primary DNA sequence at CGIs directly influence chromatin modification. Thus CGIs are not passive footprints of other cellular mechanisms, but play an active role in setting up local chromatin structure. However, the relative contribution of CpG frequency versus G+C content remains unclear. Therefore a sequence was generated that contains low levels of CpGs, comparable to the bulk genome, but has a G+C content similar to that of CGIs (Low CpG / High G+C). When this sequence was inserted into a gene desert neither marks of H3K4me3 or H3K27me3 were formed, indicating the importance of CpGs. Surprisingly, the reverse sequence with a high CpG frequency similar to that of CGIs and a low G+C content similar to that of the bulk genome (High CpG / Low G+C) did not establish H3K4me3 or H3K27me3 either. However, it was found that this sequence becomes heavily methylated in contrast to CGI-like sequences that remained unmethylated when introduced into a gene desert. This finding suggests that a high G+C content is important for keeping CGI-like sequences methylation free. Upon insertion of this High CpG / Low G+C sequence into mouse ES cells that were devoid of the de-novo DNA methyltransferases 3a and 3b (Dnmt3a/3b -/-) both H3K4me3 and H3K27me3 marks were established at the inserted sequence. This discovery confirms the importance of CpGs for setting up local chromatin structure.

Subjects/Keywords: 572.8; CpG islands; chromatin; polycomb; ES cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wachter, E. (2014). Influence of CpG islands on chromatin structure. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/9369

Chicago Manual of Style (16^th Edition):

Wachter, Elisabeth. “Influence of CpG islands on chromatin structure.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed March 04, 2021. http://hdl.handle.net/1842/9369.

MLA Handbook (7^th Edition):

Wachter, Elisabeth. “Influence of CpG islands on chromatin structure.” 2014. Web. 04 Mar 2021.

Vancouver:

Wachter E. Influence of CpG islands on chromatin structure. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1842/9369.

Council of Science Editors:

Wachter E. Influence of CpG islands on chromatin structure. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/9369

University of Vienna

4. Abdeen, Dana. Identification of novel regulators of sprouting angiogenesis using haploid ES cells.

Degree: 2017, University of Vienna

URL: http://othes.univie.ac.at/50257/

►

Die Expansion, Remodellierung und Reifung von Blutgefäßen ist ein wichtiges physiologisches Ereignis, das die embryonalen Entwicklungsstadien erfüllt und an der Pathophysiologie der Erwachsenen wie Augenerkrankungen… (more)

▼

Die Expansion, Remodellierung und Reifung von Blutgefäßen ist ein wichtiges physiologisches Ereignis, das die embryonalen Entwicklungsstadien erfüllt und an der Pathophysiologie der Erwachsenen wie Augenerkrankungen oder Krebsentwicklung beteiligt ist. Die Kaskade von Ereignissen, die zur Bildung neuer Blutgefäße aus bereits bestehenden führt, wird als Angiogenese bezeichnet. Viele bekannte und doch wenig verstandene Signalwege koordinieren und verfeinern den angiogenen Prozess. In diesem Zusammenhang sind genetische Funktionsverlustbildschirme ein wirkungsvolles Werkzeug, um die Rolle möglicher Spieler aufzudecken. Unser Ziel war es, neue angiogene Regulatoren zu finden, indem wir In-vitro-Keimungsmodelle entwickeln, die die Angiogenese von Sprossen in vivo nachahmen. Wir haben dementsprechend 32 Kandidatengene ausgewählt und eine vergleichende Studie durchgeführt, die Wildtyp- und Mutanten-Genfunktionen umfasst. Der Vergleich basierte auf der Aufklärung der Auswirkungen von Genmutationen auf die sprießenden Phänotypen in 3D-Kollagengelen. Zu diesem Zweck verwendeten wir bedingt mutagenisierte haploide murine ES-Zellen. Für jedes erhaltene Kandidatengen erzeugten wir Schwesterklone, die sich nur darin unterschieden, das Gen von Interesse entweder in der mutierten oder in der Wildtypform unter Verwendung von Cre-Rekombinationstechnologien zu tragen. Wir führten auch Kompetitionsstudien durch, indem wir gleichartige Mischungen von Wildtyp- und Mutantenklonen subkutan in immunsupprimierte Mäuse injizierten und ihren differentiellen Beitrag zur Teratomvaskulatur mittels Fluoreszenz-aktivierter Zellsortierung (FACS) -Analyse und Immunhistochemie validierten. Zusammenfassend zeigten unsere Ergebnisse, dass die Mutagenese von 13 Kandidatengenen zu einer signifikanten Abweichung ihrer sprossenden Phänotypen im Vergleich zu ihren Wildtypschwestern führte, die entweder eine verminderte oder eine erhöhte Keimfähigkeit aufwiesen. Wichtig ist, dass der sprießende Phänotyp unserer Top-Hits in In-vivo-Experimenten bestätigt wurde. Daher haben wir die Funktion von mehreren neuartigen Angiogenese-Genen identifiziert und validiert.

Blood vessel expansion, remodelling and maturation is a major physiological event that fulfils the embryonic developmental stages and takes part in adult pathophysiology such as ocular diseases or cancer development. The cascade of events that leads to the formation of new blood vessels from already existing ones is termed angiogenesis. Many known and yet poorly understood pathways orchestrate and fine tune the angiogenic process. In this context, genetic loss-of-function screens are a powerful tool to uncover the role of possible players. Our goal was to find novel angiogenic regulators by designing in vitro sprouting models that mimic sprouting angiogenesis in vivo. We accordingly chose 32 candidate genes and performed a comparative study comprising wildtype and a mutant gene functions. The comparison was based on elucidating the effect of gene mutations on the sprouting phenotypes in 3D collagen…

Subjects/Keywords: 42.13 Molekularbiologie; Angiogenesis / Haploid ES cells / Hyposprouters / Hypersprouters; Angiogenesis / Haploid ES cells / Hyposprouters / Hypersprouters

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APA (6^th Edition):

Abdeen, D. (2017). Identification of novel regulators of sprouting angiogenesis using haploid ES cells. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/50257/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abdeen, Dana. “Identification of novel regulators of sprouting angiogenesis using haploid ES cells.” 2017. Thesis, University of Vienna. Accessed March 04, 2021. http://othes.univie.ac.at/50257/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abdeen, Dana. “Identification of novel regulators of sprouting angiogenesis using haploid ES cells.” 2017. Web. 04 Mar 2021.

Vancouver:

Abdeen D. Identification of novel regulators of sprouting angiogenesis using haploid ES cells. [Internet] [Thesis]. University of Vienna; 2017. [cited 2021 Mar 04]. Available from: http://othes.univie.ac.at/50257/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abdeen D. Identification of novel regulators of sprouting angiogenesis using haploid ES cells. [Thesis]. University of Vienna; 2017. Available from: http://othes.univie.ac.at/50257/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

5. Jin, Xin. Towards differentiation of mouse embryonic stem cells to thymic epithelial progenitor cells.

Degree: PhD, 2013, University of Edinburgh

URL: http://hdl.handle.net/1842/12227

► The thymus is the major site for T-cell generation and thus is important for the adaptive immune system. Development of a properly selected, functional T-cell… (more)

Subjects/Keywords: 611; thymus; TEPC; thymic epithelial cells; embryonic stem cells; ES cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jin, X. (2013). Towards differentiation of mouse embryonic stem cells to thymic epithelial progenitor cells. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/12227

Chicago Manual of Style (16^th Edition):

Jin, Xin. “Towards differentiation of mouse embryonic stem cells to thymic epithelial progenitor cells.” 2013. Doctoral Dissertation, University of Edinburgh. Accessed March 04, 2021. http://hdl.handle.net/1842/12227.

MLA Handbook (7^th Edition):

Jin, Xin. “Towards differentiation of mouse embryonic stem cells to thymic epithelial progenitor cells.” 2013. Web. 04 Mar 2021.

Vancouver:

Jin X. Towards differentiation of mouse embryonic stem cells to thymic epithelial progenitor cells. [Internet] [Doctoral dissertation]. University of Edinburgh; 2013. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1842/12227.

Council of Science Editors:

Jin X. Towards differentiation of mouse embryonic stem cells to thymic epithelial progenitor cells. [Doctoral Dissertation]. University of Edinburgh; 2013. Available from: http://hdl.handle.net/1842/12227

University of Toronto

6. Vexelshtein, Olga. DNA Methylation of Endothelial Cell-enriched Genes during in Vitro Mouse ES Cell Differentiation and the Role of TET Proteins.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/72845

►

Endothelial cell (EC)-enriched genes are regulated by numerous mechanisms, but especially epigenetic mechanisms such as DNA methylation. The current work examines the importance of TET1… (more)

Subjects/Keywords: DNA methylation; Endothelial cells; ES cells; TET proteins; 0379

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APA (6^th Edition):

Vexelshtein, O. (2016). DNA Methylation of Endothelial Cell-enriched Genes during in Vitro Mouse ES Cell Differentiation and the Role of TET Proteins. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/72845

Chicago Manual of Style (16^th Edition):

Vexelshtein, Olga. “DNA Methylation of Endothelial Cell-enriched Genes during in Vitro Mouse ES Cell Differentiation and the Role of TET Proteins.” 2016. Masters Thesis, University of Toronto. Accessed March 04, 2021. http://hdl.handle.net/1807/72845.

MLA Handbook (7^th Edition):

Vexelshtein, Olga. “DNA Methylation of Endothelial Cell-enriched Genes during in Vitro Mouse ES Cell Differentiation and the Role of TET Proteins.” 2016. Web. 04 Mar 2021.

Vancouver:

Vexelshtein O. DNA Methylation of Endothelial Cell-enriched Genes during in Vitro Mouse ES Cell Differentiation and the Role of TET Proteins. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1807/72845.

Council of Science Editors:

Vexelshtein O. DNA Methylation of Endothelial Cell-enriched Genes during in Vitro Mouse ES Cell Differentiation and the Role of TET Proteins. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/72845

University of Rochester

7. Gabay, Meital. Ric-8 Proteins Are Molecular Chaperones Required For The Biogenesis G Protein Alpha Subunits.

Degree: PhD, 2013, University of Rochester

URL: http://hdl.handle.net/1802/26791

► Ric-8A (resistance to inhibitors of cholinesterase 8A) and Ric-8B are guanine nucleotide exchange factors that enhance different heterotrimeric G protein signaling pathways by unknown mechanisms.… (more)

▼ Ric-8A (resistance to inhibitors of cholinesterase 8A) and Ric-8B are guanine nucleotide exchange factors that enhance different heterotrimeric G protein signaling pathways by unknown mechanisms. Because transgenic disruption of Ric-8A or Ric-8B in mice caused early embryonic lethality, we derived viable Ric-8A– or Ric-8B–deleted embryonic stem (ES) cell lines from blastocysts of these mice. We observed pleiotropic G protein signaling defects in Ric-8A-/- ES cells, which resulted from reduced steady-state amounts of Gαi, Gαq, and Gα13 proteins to <5% of those of wild-type cells. Only the amount of Gαs was reduced substantially in Ric-8B-/- cells. The plasma membrane residence of G proteins persisted in the absence of Ric-8 but was markedly reduced compared to that in wild-type cells. Endogenous Gαi and Gαq were efficiently translated in Ric-8A-/- cells but integrated into endomembranes poorly; however, the reduced amounts of Gα subunits that reached the membrane still bound to nascent Gβγ. Interestingly, the Gαi and Gαq proteins that remained soluble in the Ric-8A- /- cells, had a higher apparent molecular weight, suggestive towards the presence of a putative post translational modification (PTM) that is revealed when Ric-8A is absent. In addition, Gαi, Gαq, and Gβ1 proteins exhibited accelerated rates of degradation in Ric- 8A-/- cells compared to those in wild-type cells. Together, these data suggest that Ric-8 proteins are molecular chaperones required for the initial association of nascent Gα subunits with cellular membranes. From further investigation of the slower migrating Gα proteins we concluded that in the absence of Ric-8 proteins, newly synthesized Gα proteins are not properly folded and become resistant to an artifactual proteolytic event that occurs by cellular proteases. In addition, we found that Ric-8A is natively phosphorylation in mammalian cells, however some site directed mutations of predicted phosphorylated sites resulted in full rescue of G protein expression while others generated a Ric-8A protein that was unstable when expressed in ES or insect cells. We believe that phosphorylation or dephosphorylation of Ric-8A has a functional purpose that can reveal further mechanistic information in regards to the folding and initial trafficking of Gα proteins, as well as the discovery of cellular Ric-8 activators.

Subjects/Keywords: Ric-8; G Proteins; ES Cells; Folding; Chaperon

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APA (6^th Edition):

Gabay, M. (2013). Ric-8 Proteins Are Molecular Chaperones Required For The Biogenesis G Protein Alpha Subunits. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/26791

Chicago Manual of Style (16^th Edition):

Gabay, Meital. “Ric-8 Proteins Are Molecular Chaperones Required For The Biogenesis G Protein Alpha Subunits.” 2013. Doctoral Dissertation, University of Rochester. Accessed March 04, 2021. http://hdl.handle.net/1802/26791.

MLA Handbook (7^th Edition):

Gabay, Meital. “Ric-8 Proteins Are Molecular Chaperones Required For The Biogenesis G Protein Alpha Subunits.” 2013. Web. 04 Mar 2021.

Vancouver:

Gabay M. Ric-8 Proteins Are Molecular Chaperones Required For The Biogenesis G Protein Alpha Subunits. [Internet] [Doctoral dissertation]. University of Rochester; 2013. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1802/26791.

Council of Science Editors:

Gabay M. Ric-8 Proteins Are Molecular Chaperones Required For The Biogenesis G Protein Alpha Subunits. [Doctoral Dissertation]. University of Rochester; 2013. Available from: http://hdl.handle.net/1802/26791

Texas A&M University

8. Harding, Heather Darby. Oct-4 expression in equine embryonic cells.

Degree: MS, Veterinary Physiology, 2007, Texas A&M University

URL: http://hdl.handle.net/1969.1/4793

► The Oct-4 transcription factor is believed to co-regulate early embryonic development of mammals due to the correlation of its presence with the maintenance of pluripotency.… (more)

▼ The Oct-4 transcription factor is believed to co-regulate early embryonic development of mammals due to the correlation of its presence with the maintenance of pluripotency. It is commonly used as a marker for the identification of embryonic stem (ES) cells for this reason. Until 1999, Oct-4 studies were limited to in vivo-produced embryos; equine embryos have not been studied for their Oct-4 expression patterns. In addition, equine stem-like cells (defined by marker expression, induced differentiation, passage survival, and morphology) have recently been isolated from in vivo-produced embryos, but no work has been performed in horses to isolate ES cells from in vitroproduced embryos. This study investigated the expression of Oct-4 transcription factor using immunocytochemistry in 42 in vitro-produced embryos aged 1-10 days and in 5 in vivoproduced blastocysts aged 7-10 days. Effective conditions for rapid establishment of a feeder layer of equine fetal fibroblasts were established, and this feeder layer was used to grow isolated equine inner cell mass (ICM) cells from in vitro-produced embryos. The expression of Oct-4 was examined in resultant cell growths. In vitro-produced embryos less than 6 days of age showed variable staining within blastomeres of the same embryo, and the peak of variability correlated with maternal-zygotic transition. After Oct-4 staining of in vitro-produced blastocysts, no cells could be identified as an ICM based on a difference in fluorescent intensity from the other cells of the blasyocysts. However, in vitro-produced blastocysts that were subsequently cultured in vivo contained a presumptive ICM, visible based on greater fluorescent intensity of Oct-4 stain. The trophoblast of all blastocysts also stained positively for Oct-4 protein. Fibroblasts were successfully isolated from equine feti. Treatment with 20 ÃÂµg/ml of Mitomycin C arrested cell growth without causing excessive death. Fibroblasts were inactivated and frozen, then thawed as needed to establish a confluent monolayer for ICM isolation overnight. ICMs from in vitro-produced embryos formed outgrowths, but none that could be identified morphologically as ES cells. Outgrowth cells contained about 20% Oct-4 expressing cells in sporadic groupings. Assuming appropriate binding of the Oct-4 antibody, Oct-4 expressing cells (potentially indicating pluripotency) are found throughout the embryo in early development and in the feeder layer after co-culture. Advisors/Committee Members: Hinrichs, Katrin (advisor), Binas, Bert (committee member), Choi, Young Ho (committee member), Westhusin, Mark (committee member).

Subjects/Keywords: Oct-4; ES cells; pluripotency

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APA (6^th Edition):

Harding, H. D. (2007). Oct-4 expression in equine embryonic cells. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4793

Chicago Manual of Style (16^th Edition):

Harding, Heather Darby. “Oct-4 expression in equine embryonic cells.” 2007. Masters Thesis, Texas A&M University. Accessed March 04, 2021. http://hdl.handle.net/1969.1/4793.

MLA Handbook (7^th Edition):

Harding, Heather Darby. “Oct-4 expression in equine embryonic cells.” 2007. Web. 04 Mar 2021.

Vancouver:

Harding HD. Oct-4 expression in equine embryonic cells. [Internet] [Masters thesis]. Texas A&M University; 2007. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1969.1/4793.

Council of Science Editors:

Harding HD. Oct-4 expression in equine embryonic cells. [Masters Thesis]. Texas A&M University; 2007. Available from: http://hdl.handle.net/1969.1/4793

University of Bath

9. Sanchez Ripoll, Yolanda. Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouse embryonic stem cell fate.

Degree: PhD, 2011, University of Bath

URL: https://researchportal.bath.ac.uk/en/studentthesis/glycogen-synthase-kinase-3-gsk3-involvement-in-regulation-of-mouse-embryonic-stem-cell-fate(55f41abe-2856-455a-a76f-fd91e5034eea).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.550616

Subjects/Keywords: 616.02774; ES cells; GSK-3

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sanchez Ripoll, Y. (2011). Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouse embryonic stem cell fate. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/glycogen-synthase-kinase-3-gsk3-involvement-in-regulation-of-mouse-embryonic-stem-cell-fate(55f41abe-2856-455a-a76f-fd91e5034eea).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.550616

Chicago Manual of Style (16^th Edition):

Sanchez Ripoll, Yolanda. “Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouse embryonic stem cell fate.” 2011. Doctoral Dissertation, University of Bath. Accessed March 04, 2021. https://researchportal.bath.ac.uk/en/studentthesis/glycogen-synthase-kinase-3-gsk3-involvement-in-regulation-of-mouse-embryonic-stem-cell-fate(55f41abe-2856-455a-a76f-fd91e5034eea).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.550616.

MLA Handbook (7^th Edition):

Sanchez Ripoll, Yolanda. “Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouse embryonic stem cell fate.” 2011. Web. 04 Mar 2021.

Vancouver:

Sanchez Ripoll Y. Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouse embryonic stem cell fate. [Internet] [Doctoral dissertation]. University of Bath; 2011. [cited 2021 Mar 04]. Available from: https://researchportal.bath.ac.uk/en/studentthesis/glycogen-synthase-kinase-3-gsk3-involvement-in-regulation-of-mouse-embryonic-stem-cell-fate(55f41abe-2856-455a-a76f-fd91e5034eea).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.550616.

Council of Science Editors:

Sanchez Ripoll Y. Glycogen Synthase Kinase 3 (GSK-3) involvement in regulation of mouse embryonic stem cell fate. [Doctoral Dissertation]. University of Bath; 2011. Available from: https://researchportal.bath.ac.uk/en/studentthesis/glycogen-synthase-kinase-3-gsk3-involvement-in-regulation-of-mouse-embryonic-stem-cell-fate(55f41abe-2856-455a-a76f-fd91e5034eea).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.550616

University of Melbourne

10. Motazedian, Ali. Lymphocyte differentiation from pluripotent stem cells.

Degree: 2017, University of Melbourne

URL: http://hdl.handle.net/11343/191658

► Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are cell types that can undergo unlimited self-renewal and,… (more)

▼ Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are cell types that can undergo unlimited self-renewal and, in theory, have the ability to give rise to all of the different cell types in the body. These properties of PSCs make them an attractive platform for generating a variety of normal and mutated human cell types for medical related applications. In this study, we investigated methods for generating B-cells and T-cells from PSCs. B and T lymphocytes together comprise the adaptive immune system. One of the functions of T-cells is to find and destroy defective cells, a function that has been co-opted into promising cell based therapies for the treatment of CD19+ leukaemia. Similarly, antibodies produced by B-cells now underlie promising treatments for a range of malignancies, including melanoma and lung cancer. Moreover, B-lymphocytes are of particular interest as their derivation would be indicative of progress towards the development of conditions that support hematopoietic stem cells (HSC) formation, the cell type which is likely to have the most immediate clinical applications. Therefore, in vitro production of both T and B cells from PSCs could facilitate the development of a new generation of therapeutics derived from these cell types. In this project, we examined both transcription factor and growth factor mediated induction of B-lymphoid cells as well as culture conditions that supported “stromal free” T-cell development. First, starting with culture conditions that supported primitive hematopoietic differentiation of PSCs, we investigated the effect of over-expressing B-lineage specific transcription factors in an inducible manner. Our experiments showed that, during the emergence of primitive blood cells, forced expression of PAX5 alone in CD34+ hematopoietic progenitors up-regulated the B-cell marker CD19 and, paradoxically, promoted the appearance of Glycophorin A expressing erythroid lineage cells. However, CD10+CD19+ B-cell progenitors were not observed, indicating that in the context of the primitive blood development, CD34+ progenitors were not competent to execute the instructions carried by PAX5. Second, we investigated the lymphogenic characteristics of AGM-like structures developed during a definitive hematopoiesis differentiation protocol established in laboratory. We showed that, T-lineage specification could occur in the absence of exogenous Notch1 ligands and without the need for purification of CD34+ progenitors by FACS. This finding will pave the way for a scalable method for generating T-cells for both research and for potential clinical applications. Lastly, having seen how AGM-like structures facilitated T-cell differentiation, we revisited our initial goal of B-lymphocyte differentiation from PSCs. This time, using growth factor directed differentiation, we were able to demonstrate reproducible generation of B-lymphoid progenitors from multiple PSC lines. Taken together, we hope our findings will aid…

Subjects/Keywords: pluripotent stem cells; iPS; ES; lymphocyte; T-cell; B-cell

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Motazedian, A. (2017). Lymphocyte differentiation from pluripotent stem cells. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/191658

Chicago Manual of Style (16^th Edition):

Motazedian, Ali. “Lymphocyte differentiation from pluripotent stem cells.” 2017. Doctoral Dissertation, University of Melbourne. Accessed March 04, 2021. http://hdl.handle.net/11343/191658.

MLA Handbook (7^th Edition):

Motazedian, Ali. “Lymphocyte differentiation from pluripotent stem cells.” 2017. Web. 04 Mar 2021.

Vancouver:

Motazedian A. Lymphocyte differentiation from pluripotent stem cells. [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/11343/191658.

Council of Science Editors:

Motazedian A. Lymphocyte differentiation from pluripotent stem cells. [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/191658

University of Edinburgh

11. Zhang, Jingchao. Functional analysis of the role of the Nanog tryptophan repeat in ES cells.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/15972

► Nanog is a transcription factor that plays a central part in the gene regulatory network that maintains and induces pluripotency of embryonic stem cells (ESCs).… (more)

▼ Nanog is a transcription factor that plays a central part in the gene regulatory network that maintains and induces pluripotency of embryonic stem cells (ESCs). However, the molecular basis by which Nanog achieves its functions is not fully understood. At the centre of C-terminal domain of Nanog a tryptophan repeat (WR) is located, comprising 10 penta-peptide repeats each starting with a tryptophan. A mutant form of Nanog (Nanog-W10A) in which all 10 tryptophan residues have been substituted by alanine has an impaired capacity to drive LIF-independent self-renewal and a reduced efficiency in reprogramming primed epiblast stem cells to naïve pluripotency. To understand how the WR contributes to Nanog function, Nanog-W10A-ERT2 was introduced into Nanog null cells. Upon hydroxytamoxifen addition, the Nanog-ERT2 fusion proteins were detected on chromatin within 1 hour, allowing a comparison of genome-wide transcriptional responses to Nanog and Nanog-W10A by microarray. When treated with LIF, Nanog-W10A can activate most of Nanog targets as efficiently as Nanog. In contrast, Nanog-W10A did not efficiently repress most Nanog targets, including Otx2 and Tcf15 that were previously suggested to prime ESCs for differentiation. The microarray experiments performed in the absence of LIF signalling showed that Nanog and LIF co-regulate an extensive list of targets, including Klf4 and Mras. When LIF is absent, wildtype Nanog can still activate pro-self-renewal factors, including Esrrb and repress differentiation-priming factor, such as Tcf15 and Otx2. In contrast, in the absence of LIF, the activation of pro-self-renewal factors Klf4 and Mras is reduced. In addition, activation of Esrrb by Nanog-W10A induction delays but does not prevent differentiation. These effects allow the de-repression of Otx2 and Tcf15 by Nanog-W10A to dominate. Therefore, the function of Nanog is not only mediated by the activation of pro-self-renewal genes, but also repression of pro-differentiation signals. The functional significance of the repression of Nanog targets was further exemplified by the robust capacity of Otx2 to dominate over the self-renewal signals and to drive differentiation. The Otx2 protein is a direct interacting partner of Nanog that binds the Nanog WR tryptophan residues. The previously identified Otx2 “tail domain” comprises two imperfectly aligned repeats and aromatic residues of each repeat align with aromatic residues of the Sox2 “SXS/TY” motif previously identified to mediate the interaction between Sox2 and Nanog. Aromatic residues of Otx2 were demonstrated to directly interact with both Nanog and Sox2. The interactions between Otx2, Nanog and Sox2 are essential for Otx2 functions in driving ESCs differentiation, as Otx2 mutants with alanine substitutions of the aromatic residues in both or either of the repeats have reduced efficiency to drive differentiation. As Nanog and Sox2 may co-occupy many loci important in maintaining ESC self-renewal, Otx2 may be able to “read” the Nanog/Sox2 co-binding sites to dissolve the…

Subjects/Keywords: 616.02; ES cells; Nanog; Otx2; tryptophan repeats; pluripotency

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, J. (2016). Functional analysis of the role of the Nanog tryptophan repeat in ES cells. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/15972

Chicago Manual of Style (16^th Edition):

Zhang, Jingchao. “Functional analysis of the role of the Nanog tryptophan repeat in ES cells.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed March 04, 2021. http://hdl.handle.net/1842/15972.

MLA Handbook (7^th Edition):

Zhang, Jingchao. “Functional analysis of the role of the Nanog tryptophan repeat in ES cells.” 2016. Web. 04 Mar 2021.

Vancouver:

Zhang J. Functional analysis of the role of the Nanog tryptophan repeat in ES cells. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1842/15972.

Council of Science Editors:

Zhang J. Functional analysis of the role of the Nanog tryptophan repeat in ES cells. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/15972

12. Osorno Hernandez, Carlos Rodrigo. Transcription factor heterogeneity in epiblast pluripotency.

Degree: PhD, 2013, University of Edinburgh

URL: http://hdl.handle.net/1842/8081

► Pluripotency is the ability of a cell to differentiate into derivatives of all three somatic lineages and germ cells. In vivo, pluripotent cells exist transiently… (more)

▼ Pluripotency is the ability of a cell to differentiate into derivatives of all three somatic lineages and germ cells. In vivo, pluripotent cells exist transiently in the epiblast of the developing embryo and in rare tumour cells. In vitro, pluripotent cells have been isolated and propagated from teratocarcinomas (EC cells), preimplantation epiblast (ES cells) and post-implantation epiblast (EpiSCs). Pluripotency is governed by a gene regulatory network centred on the triumvirate of transcription factors Oct4, Sox2 and Nanog. Interestingly, transcription factors that are important to direct pluripotent cell identity are not all equally distributed throughout the pluripotent cell population. While Oct4 levels are relatively homogeneous, other transcription factors, such as Nanog, are more heterogeneously expressed. Additionally, an increasing body of evidence indicates that extrinsic cues also play a critical role in the establishment and maintenance of pluripotency. Using biochemical and genetic tools in mouse ES cells, the role of FGF signaling and Sox2 levels on heterogeneous Nanog expression was examined. Interference with FGF or ERK activity by genetic ablation or signal inhibition, promoted high, homogenous Nanog expression and enhanced self-renewal. This is consistent with reports showing that similar manipulations reduced the ability of ES cells to commit to differentiation. Moreover, ES cells with reduced Sox2 levels displayed greater heterogeneity for Nanog expression than wild-type ES cells. Pluripotency is lost in the mouse embryo around E8.5, however, the precise timing and mechanism involved in this process has not yet been defined. Here it is shown that pluripotency is extinguished at the onset of somitogenesis, coincident with reduced expression and chromatin accessibility of Oct4 and Nanog regulatory regions. Prior to somitogenesis, the expression of both Nanog and Oct4 is regionalized. Interestingly, pluripotency tracks the in vivo level of Oct4, this correlation does not hold true for Nanog. However, Nanog expression reports on pluripotent cells. Indeed, ectopic Oct4 expression in somitogenesis-stage tissue provokes rapid reopening of Oct4 and Nanog chromatin, Nanog re-expression and resuscitation of moribund pluripotency. Competence to re-activate the pluripotency network upon enforced Oct4 expression is gradually lost with the progression of embryonic development. ES cells and EpiSCs are two distinct pluripotent populations as they show differences in their ability to undergo clonal propagation, re-colonize embryos, growth factor responsiveness, morphology and gene regulatory networks. It is possible to harness this differential growth factor responsiveness to convert ES cells into EpiSCs. Conversely, EpiSC can be reverted back to ES cell pluripotency through the overexpression of a small number of transcription factors. The inter-conversion of ES cells and EpiSCs has been documented, but detailed analyses of the changes that occur during such transitions had not been performed. The current work…

Subjects/Keywords: 571.6; pluripotency; ES cells

…38 2.1 Culture and manipulation of mouse ES cells… …39 2.1.1.4 Reagents for passaging of ES cells… …41 2.1.3 Long-term storage of mouse ES cells… …43 2.1.4 Thawing mouse ES cells… …43 2.1.5 Transfection of DNA into mouse ES cells…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Osorno Hernandez, C. R. (2013). Transcription factor heterogeneity in epiblast pluripotency. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/8081

Chicago Manual of Style (16^th Edition):

Osorno Hernandez, Carlos Rodrigo. “Transcription factor heterogeneity in epiblast pluripotency.” 2013. Doctoral Dissertation, University of Edinburgh. Accessed March 04, 2021. http://hdl.handle.net/1842/8081.

MLA Handbook (7^th Edition):

Osorno Hernandez, Carlos Rodrigo. “Transcription factor heterogeneity in epiblast pluripotency.” 2013. Web. 04 Mar 2021.

Vancouver:

Osorno Hernandez CR. Transcription factor heterogeneity in epiblast pluripotency. [Internet] [Doctoral dissertation]. University of Edinburgh; 2013. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1842/8081.

Council of Science Editors:

Osorno Hernandez CR. Transcription factor heterogeneity in epiblast pluripotency. [Doctoral Dissertation]. University of Edinburgh; 2013. Available from: http://hdl.handle.net/1842/8081

University of Toronto

13. Norman, Andreea. Characterization of ES Cell-derived Cortical Radial Precursor Differentiation.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/25879

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Murine neural precursor cells have been a well studied model for neural cell fate determination and stem cell function both in vivo and in primary… (more)

Subjects/Keywords: cortical precursors; neural stem cells; mouse ES cells; corticogenesis; in vitro differentiation; retinoic acid; 0317

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Norman, A. (2010). Characterization of ES Cell-derived Cortical Radial Precursor Differentiation. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25879

Chicago Manual of Style (16^th Edition):

Norman, Andreea. “Characterization of ES Cell-derived Cortical Radial Precursor Differentiation.” 2010. Masters Thesis, University of Toronto. Accessed March 04, 2021. http://hdl.handle.net/1807/25879.

MLA Handbook (7^th Edition):

Norman, Andreea. “Characterization of ES Cell-derived Cortical Radial Precursor Differentiation.” 2010. Web. 04 Mar 2021.

Vancouver:

Norman A. Characterization of ES Cell-derived Cortical Radial Precursor Differentiation. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1807/25879.

Council of Science Editors:

Norman A. Characterization of ES Cell-derived Cortical Radial Precursor Differentiation. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25879

University of Edinburgh

14. Cowan, Gillian. Fetal germ cell differentiation and the impact of the somatic cells.

Degree: PhD, 2009, University of Edinburgh

URL: http://hdl.handle.net/1842/4164

► Specification of a germ cell lineage and appropriate maturation are essential for the transfer of genetic information from one generation to the next. Germ cells… (more)

▼ Specification of a germ cell lineage and appropriate maturation are essential for the transfer of genetic information from one generation to the next. Germ cells form from pluripotent precursor cells that migrate into the gonadal ridge and undergo commitment to either the female or male lineage. In the fetal ovary, germ cells enter meiotic prophase I, then arrest at the diplotene stage; in the testis germ cells do not begin meiosis until puberty. Abnormal differentiation of germ cells can result in malignant transformation. Somatic cells play a key role in modulating the developmental fate of the germ cells. Research into germ cell development during fetal life has almost exclusively focused on studies in rodents, but we, and others, have reported several fundamental differences in the expression of germ cell specific markers in the human compared with the mouse. The studies described in this thesis have investigated germ cell-specific gene expression and the possible impact of the somatic cells during development. This was achieved by studying human fetal gonads obtained during the 1st and 2nd trimesters of pregnancy and through the use of both wild-type and mutant mouse ES cell lines. Studies on germ cells in the human fetal testis have extended the findings of others, and confirmed that germ cell populations at different stages of maturation co-exist in the human fetal testis, a situation that is in contrast to that in rodents. For example expression of M2A and AP2γ was restricted to the OCT4-positive gonocyte population, while VASA and NANOS1 were localised exclusively to the to the OCT4-negative prespermatogonia. DAZL was expressed in both populations. Analysis also revealed that both the gonocyte and prespermatogonial populations proliferate throughout the 2nd trimester. Recent studies have implicated retinoic acid (RA) in the control of meiotic entry in germ cells of the fetal mouse ovary. In this study we demonstrated for the first time that two genes implicated in the action of RA in mouse gonad, STRA8 and NANOS2, are also expressed in a similar sexspecific- manner in the human fetal gonads, and that the RA receptors are present in both somatic and germ cells suggesting that RA may regulate germ cell function in the human as well as the mouse. However, whilst the mesonephros appears to be the primary site of RA synthesis in the mouse our initial studies indicate that in the human the gonad itself may be a more likely site of RA biosynthesis. In the fetal mouse testis, RA is degraded by the enzyme Cyp26b1 present in the somatic cells and germ cells do not enter meiosis, our novel findings suggest that CYP26B1 is more abundant in the human fetal ovary than the testis, suggesting that meiotic entry may be controlled by an alternative signalling pathway in the human. One of the methods that can aid our understanding of somatic cell gene expression in the gonad is in vitro culture. To date, there have been no published reports of the successful in vitro culture of somatic cells from the human fetal testis. In the…

Subjects/Keywords: 612.6; germ cells; sertoli cells; ES cells; meiosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cowan, G. (2009). Fetal germ cell differentiation and the impact of the somatic cells. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/4164

Chicago Manual of Style (16^th Edition):

Cowan, Gillian. “Fetal germ cell differentiation and the impact of the somatic cells.” 2009. Doctoral Dissertation, University of Edinburgh. Accessed March 04, 2021. http://hdl.handle.net/1842/4164.

MLA Handbook (7^th Edition):

Cowan, Gillian. “Fetal germ cell differentiation and the impact of the somatic cells.” 2009. Web. 04 Mar 2021.

Vancouver:

Cowan G. Fetal germ cell differentiation and the impact of the somatic cells. [Internet] [Doctoral dissertation]. University of Edinburgh; 2009. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/1842/4164.

Council of Science Editors:

Cowan G. Fetal germ cell differentiation and the impact of the somatic cells. [Doctoral Dissertation]. University of Edinburgh; 2009. Available from: http://hdl.handle.net/1842/4164

Univerzitet u Beogradu

15. Mitić, Ivana D., 1981-. Modulacija eksperimentalnog autoimunskog encefalomijelitisa primenom ekskretorno-sekretornih antigena parazita Trichinella spiralis.

Degree: Medicinski fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:14182/bdef:Content/get

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Medicina - Imunologija / Medicine - Immunology

Savremena istraživanja na eksperimentalnim modelima autoimunskih bolesti ukazuju da infekcija helmintom Trichinella spiralis (T. spiralis), inhibira nastanak ili… (more)

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Medicina - Imunologija / Medicine - Immunology

Savremena istraživanja na eksperimentalnim modelima autoimunskih bolesti ukazuju da infekcija helmintom Trichinella spiralis (T. spiralis), inhibira nastanak ili ublažava simptome autoimunskih poremećaja kao što su eksperimentalni autoimunski encefalomijelitis (EAE), eksperimentalni kolitis i dijabetes melitus tip 1. Tokom infekcije parazitom T. spiralis ekskretorno-sekretorni metabolički produkti mišićnih larvi (ES L1) su ključni za interakciju sa organizmom domaćina. Stoga je pretpostavka da su upravo ES L1 produkti odgovorni za modulaciju toka autoimunskih oboljenja. Međutim, tačna uloga ES L1 antigena ovog parazita u modulaciji bolesti, kao i mehanizmi te modulacije nisu još uvek u potpunosti razjašnjeni. Predmet ovog istraživanja bilo je ispitivanje uticaja direktne primene ES L1 produkata kao i dendritskih ćelija (DC) stimulisanih ovim antigenima na tok i modulaciju EAE-a kod DA pacova, sa ciljem da se utvrde mehanizmi imunomodulacije kao i uloga dendritskih ćelija u njima. Ovde prikazani rezultati, dobijeni u in vitro studijama na modelu DC izolovanih iz kostne srži DA pacova, potvrdili su da ES antigeni T. spiralis dovode do delimičnog sazrevanja DC koje se mogu označiti kao tolerogene DC koje se odlikuju niskom produkcijom pro-inflamatornog citokina IL-12p70 i povećanom produkcijom anti-inflamatornog citokina IL-10. U ovom radu prvi put je ustanovljeno da primena DC stimulisanih ES L1 antigenima T. spiralis (DC/ES L1) ima inhibitorni efekat na razvoj EAE-a kod DA pacova kao i da je ovaj efekat dozno zavisan. Najveći uticaj na smanjenje težine bolesti ostvaren je primenom doze od 5 x 106 DC/ES L1. Mehanizam koji je u osnovi ovog fenomena zasniva se na polarizaciji imunskog odgovora u pravcu Th2 i regulatornog tipa praćenog povećanom produkcijom Th2 i anti-inflamatornih citokina (IL-4, IL-10 i TGF-β), a smanjenom produkcijom pro-inflamatornih citokina IFN-γ i IL-17. Takođe, dolazi do povećanja procentualne zastupljenosti CD4+CD25+Foxp3+ regulatornih T-limfocita (Treg) na sistemskom nivou u slezini i na nivou ciljnog organa, u CNS-u...

Advisors/Committee Members: Marković, Miloš, 1969-.

Subjects/Keywords: Trichinella spiralis; EAE; ES L1; immune response; dendritic cells; regulatory T lymphocytes

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APA (6^th Edition):

Mitić, Ivana D., 1. (2016). Modulacija eksperimentalnog autoimunskog encefalomijelitisa primenom ekskretorno-sekretornih antigena parazita Trichinella spiralis. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:14182/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mitić, Ivana D., 1981-. “Modulacija eksperimentalnog autoimunskog encefalomijelitisa primenom ekskretorno-sekretornih antigena parazita Trichinella spiralis.” 2016. Thesis, Univerzitet u Beogradu. Accessed March 04, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:14182/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mitić, Ivana D., 1981-. “Modulacija eksperimentalnog autoimunskog encefalomijelitisa primenom ekskretorno-sekretornih antigena parazita Trichinella spiralis.” 2016. Web. 04 Mar 2021.

Vancouver:

Mitić, Ivana D. 1. Modulacija eksperimentalnog autoimunskog encefalomijelitisa primenom ekskretorno-sekretornih antigena parazita Trichinella spiralis. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Mar 04]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:14182/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mitić, Ivana D. 1. Modulacija eksperimentalnog autoimunskog encefalomijelitisa primenom ekskretorno-sekretornih antigena parazita Trichinella spiralis. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:14182/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. SLIM MZOUGHI. CHARACTERIZATION OF THE PR-DOMAIN PROTEIN PRDM15: ROLE IN DEVELOPMENT AND CANCER.

Degree: 2015, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/121262

Subjects/Keywords: PRDM15; ES cells; development; Transcription; chromatin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MZOUGHI, S. (2015). CHARACTERIZATION OF THE PR-DOMAIN PROTEIN PRDM15: ROLE IN DEVELOPMENT AND CANCER. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/121262

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MZOUGHI, SLIM. “CHARACTERIZATION OF THE PR-DOMAIN PROTEIN PRDM15: ROLE IN DEVELOPMENT AND CANCER.” 2015. Thesis, National University of Singapore. Accessed March 04, 2021. http://scholarbank.nus.edu.sg/handle/10635/121262.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MZOUGHI, SLIM. “CHARACTERIZATION OF THE PR-DOMAIN PROTEIN PRDM15: ROLE IN DEVELOPMENT AND CANCER.” 2015. Web. 04 Mar 2021.

Vancouver:

MZOUGHI S. CHARACTERIZATION OF THE PR-DOMAIN PROTEIN PRDM15: ROLE IN DEVELOPMENT AND CANCER. [Internet] [Thesis]. National University of Singapore; 2015. [cited 2021 Mar 04]. Available from: http://scholarbank.nus.edu.sg/handle/10635/121262.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

MZOUGHI S. CHARACTERIZATION OF THE PR-DOMAIN PROTEIN PRDM15: ROLE IN DEVELOPMENT AND CANCER. [Thesis]. National University of Singapore; 2015. Available from: http://scholarbank.nus.edu.sg/handle/10635/121262

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. VINOTH KUMAR S/O JAYASEELAN. ROLE OF HUMAN EMBRYONIC STEM CELLS AND DERIVED PROGENIES IN GENOTOXICITY TESTING.

Degree: 2011, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/26141

Subjects/Keywords: ES cells; Toxicology testing; Genotoxicity Testing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

JAYASEELAN, V. K. S. (2011). ROLE OF HUMAN EMBRYONIC STEM CELLS AND DERIVED PROGENIES IN GENOTOXICITY TESTING. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/26141

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

JAYASEELAN, VINOTH KUMAR S/O. “ROLE OF HUMAN EMBRYONIC STEM CELLS AND DERIVED PROGENIES IN GENOTOXICITY TESTING.” 2011. Thesis, National University of Singapore. Accessed March 04, 2021. http://scholarbank.nus.edu.sg/handle/10635/26141.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

JAYASEELAN, VINOTH KUMAR S/O. “ROLE OF HUMAN EMBRYONIC STEM CELLS AND DERIVED PROGENIES IN GENOTOXICITY TESTING.” 2011. Web. 04 Mar 2021.

Vancouver:

JAYASEELAN VKS. ROLE OF HUMAN EMBRYONIC STEM CELLS AND DERIVED PROGENIES IN GENOTOXICITY TESTING. [Internet] [Thesis]. National University of Singapore; 2011. [cited 2021 Mar 04]. Available from: http://scholarbank.nus.edu.sg/handle/10635/26141.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

JAYASEELAN VKS. ROLE OF HUMAN EMBRYONIC STEM CELLS AND DERIVED PROGENIES IN GENOTOXICITY TESTING. [Thesis]. National University of Singapore; 2011. Available from: http://scholarbank.nus.edu.sg/handle/10635/26141

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

18. Bekhuis, L.M. Evaluation of Midbrain Dopaminergic Neuron Specification in Rat ES Cell Derived Neurons.

Degree: 2013, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/281018

► Parkinson's disease (PD) is a chronic, neurodegenerative disorder, which arises as a result of the progressive loss of midbrain dopaminergic (mDA) neurons in the substantia… (more)

Subjects/Keywords: Diergeneeskunde; Parkinson's disease; midbrain dopaminergic neurons; embryonic stem cells; rat ES cells; MEK/ERK; RT-PCR; midbrain development

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APA (6^th Edition):

Bekhuis, L. M. (2013). Evaluation of Midbrain Dopaminergic Neuron Specification in Rat ES Cell Derived Neurons. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/281018

Chicago Manual of Style (16^th Edition):

Bekhuis, L M. “Evaluation of Midbrain Dopaminergic Neuron Specification in Rat ES Cell Derived Neurons.” 2013. Masters Thesis, Universiteit Utrecht. Accessed March 04, 2021. http://dspace.library.uu.nl:8080/handle/1874/281018.

MLA Handbook (7^th Edition):

Bekhuis, L M. “Evaluation of Midbrain Dopaminergic Neuron Specification in Rat ES Cell Derived Neurons.” 2013. Web. 04 Mar 2021.

Vancouver:

Bekhuis LM. Evaluation of Midbrain Dopaminergic Neuron Specification in Rat ES Cell Derived Neurons. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2021 Mar 04]. Available from: http://dspace.library.uu.nl:8080/handle/1874/281018.

Council of Science Editors:

Bekhuis LM. Evaluation of Midbrain Dopaminergic Neuron Specification in Rat ES Cell Derived Neurons. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/281018

Indian Institute of Science

19. Verma, Isha. Insights into Differentiation of Mouse Pluripotent Stem Cells to Neural Lineage.

Degree: PhD, Faculty of Science, 2017, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2885

► Pluripotent stem cells (PSCs: ESCs and iPSCs) provide an excellent model system for studying neural development and function. These cells also serve as a reliable… (more)

▼ Pluripotent stem cells (PSCs: ESCs and iPSCs) provide an excellent model system for studying neural development and function. These cells also serve as a reliable source of cell replacement for the treatment of various neurodegenerative diseases and disorders. In view of these applications of PSCs, multiple protocols have been developed to direct their differentiation into neural lineage. However, many of these protocols are limiting in terms of (a) low efficiency of generation of neural cells after long-term culture, (b) requirement of exogenous factors to induce and enhance neural differentiation and (c) supplementation of PSC culture medium with serum. Therefore, in the present study, attempts were made to achieve enhanced efficiency of neural differentiation of PSCs in the absence of exogenous molecules by employing a defined culture medium containing knockout serum replacement (KSR). KSR-based culture system was tested with our in-house-derived EGFP-transgenic ‘GS-2’ ES-cell and ‘N9’ iPS-cell lines and the wild-type ‘D3’ ES-cell line. In KSR medium, PSC-derived EBs predominantly generated neural cells from their post-attachment outgrowths and the complexity of neural networks increased as the culture progressed. Molecular phenotyping of PSC-derived neural cells was performed based on the expression of neural markers both at the mRNA and protein levels. qPCR analysis revealed the expression of markers corresponding to multiple neural cell types, including glutamatergic, GABAergic, cholinergic, serotonergic and dopaminergic neurons, astrocytes and oligodendrocytes, at various time points during the culture. RNA expression studies were confirmed via immunocytochemical analysis of the expression of neural markers. On day 15 of culture, FACS quantitation revealed the efficient generation of NES+ neural progenitors (~16-18%), MAP2+ mature neurons (~12-26%) and GFAP+ astrocytes (~30-63%) from the three PSC lines. Functional assessment of the generated neurons was performed by electrophysiological analysis of passive (RMP) and active (threshold, amplitude, FWHM and outward and inward currents) membrane properties. In order to investigate the role of default pathway in neural differentiation of PSCs in KSR medium, various strategies were employed. GS-2 ES-cells were cultured in the presence of different serum-free supplements; predominant differentiation into neural lineage was achieved in the B27-supplemented medium. The supplementation of KSR medium with BMP4 failed to show any effect of neural differentiation of GS-2 ES-cells. Also, EBs were switched between KSR- and FBS-supplemented culture conditions on day 2 or day 5 of culture. These experiments indicated that KSR medium promoted the generation of neural cell fates at the expense of differentiation to non-neural lineages. Interestingly, differentiation of P19 EC-cells in KSR medium also resulted in the predominant neural differentiation. These experiments collectively suggested the importance of default pathway in neural differentiation of PSCs in KSR medium.… Advisors/Committee Members: Seshagiri, Polani B (advisor).

Subjects/Keywords: Mouse Pluripotent Stem Cells; Neural Lineage; Neural Differentiation of PSCs; Neural Differentiation; ES-cells; Neural Cell; Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Verma, I. (2017). Insights into Differentiation of Mouse Pluripotent Stem Cells to Neural Lineage. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2885

Chicago Manual of Style (16^th Edition):

Verma, Isha. “Insights into Differentiation of Mouse Pluripotent Stem Cells to Neural Lineage.” 2017. Doctoral Dissertation, Indian Institute of Science. Accessed March 04, 2021. http://etd.iisc.ac.in/handle/2005/2885.

MLA Handbook (7^th Edition):

Verma, Isha. “Insights into Differentiation of Mouse Pluripotent Stem Cells to Neural Lineage.” 2017. Web. 04 Mar 2021.

Vancouver:

Verma I. Insights into Differentiation of Mouse Pluripotent Stem Cells to Neural Lineage. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2017. [cited 2021 Mar 04]. Available from: http://etd.iisc.ac.in/handle/2005/2885.

Council of Science Editors:

Verma I. Insights into Differentiation of Mouse Pluripotent Stem Cells to Neural Lineage. [Doctoral Dissertation]. Indian Institute of Science; 2017. Available from: http://etd.iisc.ac.in/handle/2005/2885

Indian Institute of Science

20. Abbey, Deepti. Insights Into Molecular Regulation Of Cardiomyocyte Differentiation Of Mouse Pluripotent Stem Cells.

Degree: PhD, Faculty of Science, 2015, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2455

► Pluripotent stem cells (PSCs) are specialized cells, which have remarkable ability to maintain in an undifferentiated state and are capable of undergoing differentiation to three… (more)

▼ Pluripotent stem cells (PSCs) are specialized cells, which have remarkable ability to maintain in an undifferentiated state and are capable of undergoing differentiation to three germ-layer lineage cell types, under differentiation-enabling conditions. PSCs include embryonic stem (ES)-cells, embryonal carcinoma (EC)-cells and embryonic germ (EG)-cells. ES-cells are derived from the inner cell mass (ICM) of day 3.5 blastocysts (mouse). On the other hand, EC- and EG-cells have different source of origin and exhibit some differences in terms of their differentiation abilities and culture requirements. These PSCs act as an ideal in-vitro model system to study early mammalian development and cell differentiation and, they could potentially be used for experimental cell-based therapy for a number of diseases. However, one of the problems encountered is the immune rejection of transplanted cells. For this, immune-matched induced pluripotent stem (iPS)-cells have been derived from somatic cells, by forced expression of a few stemness genes. Although, human PSCs lines are being experimented, their cell-therapeutic potential is still far from being thoroughly tested due to lack of our understanding regarding lineage-specific differentiation, homing and structural-functional integration of differentiated cell types in the host environment. To understand these mechanisms, it is desirable to have fluorescently-marked PSCs and their differentiated cell-types, which could facilitate experimental cell transplantation studies. In this regard, our laboratory has earlier generated enhanced green fluorescent protein (EGFP)-expressing FVB/N transgenic ‘green’ mouse: GU-3 line (Devgan et al., 2003). This transgenic mouse has been an excellent source of intrinsically green fluorescent cell types. Recently, we have derived a ‘GS-2’ ES-cell line from the GU-3 mouse line (Singh et al., 2012). Additionally, we envisaged the need for developing an iPS-cell line from the GU-3 mouse and then use them for studying cell differentiation. Thus, aims of the study described in the thesis are to: (1) develop an experimental system to derive EGFP-expressing fluorescently-marked iPS-cell line from a genetically non-permissive FVB/N mouse strain, characterize the established iPS-cell line and achieve differentiation of various cell types from EGFP-expressing iPS-cell line; (2) to study differentiation phenomenon, in particular to cardiac lineage, using select-cardiogenesis modulators and (3) to assess the gene-expression profiles and signaling system associated with cardiomyocyte differentiation of PSCs. This thesis is divided into four chapters with the 1st chapter being a review of literature followed by three data chapters. In the chapter I of the thesis, a comprehensive up-to¬date review of literature is provided pertaining to PSCs, their classification, derivation strategies especially for reprogramming of somatic cells for iPSC generation, their differentiation potential and characterization, particularly to cardiac lineage. Various molecular… Advisors/Committee Members: Seshagiri, Polani B (advisor).

Subjects/Keywords: Molecular Regulation; Cardiac Differentiation; Cell Differentiation; Pluripotent Stem Cells; Embryonic Stem Cells; Embryonal Carcinoma Cells; Cardiomyocytes; Cardiomyocyte Differentiation; Mouse Pluripotent Stem Cells; Pluripotent Stem Cells (PSCs); Stem Cell Research; Embryonic Germ Cells; EC-cells; ES-cells; Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abbey, D. (2015). Insights Into Molecular Regulation Of Cardiomyocyte Differentiation Of Mouse Pluripotent Stem Cells. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2455

Chicago Manual of Style (16^th Edition):

Abbey, Deepti. “Insights Into Molecular Regulation Of Cardiomyocyte Differentiation Of Mouse Pluripotent Stem Cells.” 2015. Doctoral Dissertation, Indian Institute of Science. Accessed March 04, 2021. http://etd.iisc.ac.in/handle/2005/2455.

MLA Handbook (7^th Edition):

Abbey, Deepti. “Insights Into Molecular Regulation Of Cardiomyocyte Differentiation Of Mouse Pluripotent Stem Cells.” 2015. Web. 04 Mar 2021.

Vancouver:

Abbey D. Insights Into Molecular Regulation Of Cardiomyocyte Differentiation Of Mouse Pluripotent Stem Cells. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2015. [cited 2021 Mar 04]. Available from: http://etd.iisc.ac.in/handle/2005/2455.

Council of Science Editors:

Abbey D. Insights Into Molecular Regulation Of Cardiomyocyte Differentiation Of Mouse Pluripotent Stem Cells. [Doctoral Dissertation]. Indian Institute of Science; 2015. Available from: http://etd.iisc.ac.in/handle/2005/2455

Indian Institute of Science

21. Singh, Gurbind. Studies On Embryonic Stem Cells From Enhanced Green Fluorescent Protein Transgenic Mice : Induction Of Cardiomyocyte Differentiation.

Degree: PhD, Faculty of Science, 2013, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2116

► Genesis of life begins with the fusion of female and male haploid gametes through a process of fertilization leading to the formation of a diploid… (more)

▼ Genesis of life begins with the fusion of female and male haploid gametes through a process of fertilization leading to the formation of a diploid cell, the zygote. This undergoes successive cleavage divisions forming 2-, 4- and 8- cell embryos and their individual cells (blastomeres) are totipotent. As development proceeds, there is a gradual restriction in their totipotency, resulting in the generation of two distinct cell lineages i.e., the differentiated trophectoderm (TE) cells and the undifferentiated, inner cell mass (ICM) during blastocyst morphogenesis (Rossant and Tam 2009). During the course of development, the ICM cells can give rise to all cell types of an organism and can also provide embryonic stem (ES)-cells when cultured in vitro (Evan and Kaufman 1981). ES-cells are pluripotent cells, having the ability to self-renew indefinitely and differentiate into all the three primary germ layers (ectoderm, mesoderm and endoderm) derived-cell types. ES-cells are an excellent developmental model system to understand basic mechanisms of self-renewal, cell differentiation and function of various genes in vitro and in vivo (Capecchi 2001). Importantly, their cell derivatives could potentially be used for experimental cell-based therapy for a number of diseases. Although, human ES-cell lines have been successfully derived and differentiated to various cell types (Thomson et al., 1998; Odorico et al., 2001), their cell-therapeutic potential is far from being tested, in view of the lack of our understanding of lineage-specific differentiation, homing and structural-functional integration of differentiated cell types in the host environment. To understand these mechanisms, it is desirable to have fluorescently-marked ES-cells and their differentiated cell-types, which could facilitate experimental cell transplantation studies. In this regard, our laboratory has earlier generated enhanced green fluorescent protein (EGFP)-expressing FVB/N transgenic ‘green’ mouse, under the control of ubiquitous chicken -actin promoter (Devgan et al., 2003). This transgenic mouse has been an excellent source of intrinsically green fluorescent cell types. We have been attempting to derive ES-cell line from this transgenic mouse. Because the derivation of ES-cell line is genetic strain-dependent, with some strains being relatively permissible for ES-cell derivation while others are quite resistant (non permissive), it has been extremely difficult to derive ES-cell line from the FVB/N mouse strain. There is a need to evolve experimental strategies to derive ES-cell line from FVB/N mouse, a strain extensively used for transgenesis. Thus, the aims of the study described in the thesis are to: (1) develop an experimental system to derive EGFP-expressing fluorescently-marked ES-cell line from a non-permissive FVB/N mouse strain; (2) characterize the established ES-cell line; (3) achieve differentiation of various cell types from EGFP-expressing ES-cell line and (4) understand role of FGF signaling in cardiac differentiation from the… Advisors/Committee Members: Seshagiri, Polani B (advisor).

Subjects/Keywords: Experimental Research; Stem Cells; Transgenic Mice; Cardiomyocyte Differentiation; Embryonic Stem Cells; Enhanced Green Fluorescent Protein (EGFP); Fibroblast Growth Factor (FGF); Embryonic Stem Cell Line; ES-cells; Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Singh, G. (2013). Studies On Embryonic Stem Cells From Enhanced Green Fluorescent Protein Transgenic Mice : Induction Of Cardiomyocyte Differentiation. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2116

Chicago Manual of Style (16^th Edition):

Singh, Gurbind. “Studies On Embryonic Stem Cells From Enhanced Green Fluorescent Protein Transgenic Mice : Induction Of Cardiomyocyte Differentiation.” 2013. Doctoral Dissertation, Indian Institute of Science. Accessed March 04, 2021. http://etd.iisc.ac.in/handle/2005/2116.

MLA Handbook (7^th Edition):

Singh, Gurbind. “Studies On Embryonic Stem Cells From Enhanced Green Fluorescent Protein Transgenic Mice : Induction Of Cardiomyocyte Differentiation.” 2013. Web. 04 Mar 2021.

Vancouver:

Singh G. Studies On Embryonic Stem Cells From Enhanced Green Fluorescent Protein Transgenic Mice : Induction Of Cardiomyocyte Differentiation. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2013. [cited 2021 Mar 04]. Available from: http://etd.iisc.ac.in/handle/2005/2116.

Council of Science Editors:

Singh G. Studies On Embryonic Stem Cells From Enhanced Green Fluorescent Protein Transgenic Mice : Induction Of Cardiomyocyte Differentiation. [Doctoral Dissertation]. Indian Institute of Science; 2013. Available from: http://etd.iisc.ac.in/handle/2005/2116

University of Southern California

22. Andrianakos, Rosemary G. Roles of Klf4 in embryonic stem cells.

Degree: MS, Biochemistry & Molecular Biology, 2008, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/70866/rec/5653

► Human and mouse ES cells are defined by their ability to confer self-renewal and differentiate into various cell types, making them an invaluable tool for… (more)

Subjects/Keywords: stem cells; reprogramming somatic cells; Klf4; Oct-4; Sox-2; differentiation of Es cells; pluripotency; ES cell maintenance and self renewal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrianakos, R. G. (2008). Roles of Klf4 in embryonic stem cells. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/70866/rec/5653

Chicago Manual of Style (16^th Edition):

Andrianakos, Rosemary G. “Roles of Klf4 in embryonic stem cells.” 2008. Masters Thesis, University of Southern California. Accessed March 04, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/70866/rec/5653.

MLA Handbook (7^th Edition):

Andrianakos, Rosemary G. “Roles of Klf4 in embryonic stem cells.” 2008. Web. 04 Mar 2021.

Vancouver:

Andrianakos RG. Roles of Klf4 in embryonic stem cells. [Internet] [Masters thesis]. University of Southern California; 2008. [cited 2021 Mar 04]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/70866/rec/5653.

Council of Science Editors:

Andrianakos RG. Roles of Klf4 in embryonic stem cells. [Masters Thesis]. University of Southern California; 2008. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/70866/rec/5653

University of Michigan

23. Ramamurthy, Poornapriya. Mouse Embryonic Stem Cells Differentiated into Neuron-like Cells or Schwann Cell-like Cells for the Development of Strategies to Ameliorate Deafness.

Degree: PhD, Biomedical Engineering, 2012, University of Michigan

URL: http://hdl.handle.net/2027.42/94096

► Cochlear prostheses (CI) can restore hearing to patients with extensive sensorineural hearing loss (SNHL) by replacing lost or damaged cochlear mechanosensory hair cells (HC) with… (more)

▼ Cochlear prostheses (CI) can restore hearing to patients with extensive sensorineural hearing loss (SNHL) by replacing lost or damaged cochlear mechanosensory hair cells (HC) with an electrode array. For the CI to send meaningful acoustic information to the brain, as large a population of remaining inner ear spiral ganglion neurons (SGN) as possible must become functionally coupled to the CI. SGN-CI coupling would be enhanced by a means of inducing directed neurite extension from the remaining SGN to the CI. Macrophage migration inhibitory factor (MIF) is a neurotrophic cytokine, which is expressed in early embryogenesis in the central and peripheral nervous systems, the eye and inner ear, where it is expressed in the supporting cells that underlie the HC and in all Schwann Cells. We produced a mESC-derived neuron-like population of cells from mESC exposed to recombinant MIF that in many respects resemble SGN. Maturation of these MIF mESC-derived neuron-like cells was enhanced by Docosahexaenoic acid, a fatty acid that helps promote neuronal maturation. Such cells could be used eventually to replace lost of damaged SGN. This laboratory also produced the first embryonic stem cell (ESC) derived model of the Schwann Cell. These Schwann cells produce MIF, which is capable of inducing directional neurite outgrowth and supporting the survival of both early stage statoacoustic ganglion neurons (SAG) and their adult counterparts in the SGN as do all Schwann Cells. We have coated CIs with mESC-MIF-producing “Schwann Cells” and observed directional outgrowth and contact formation between the “Schwann Cell”-coated CI and primary mouse embryonic SAG or adult SGN to bring the neurites closer to the CI for better performance. Thus two different stem cell-based approaches have been used to address two different problems in deafness: improved integration of CI with native SGN and replacement of SGN themselves with a stem cell population molecularly engineered to resemble SGN. Advisors/Committee Members: Barald, Kate F. (committee member), Grosh, Karl (committee member), Stegemann, Jan Philip (committee member), Takayama, Shuichi (committee member), El-Sayed, Mohamed (committee member).

Subjects/Keywords: ES Cells, Neuron-like Cell, Schwann -Like Cell, Microfluidics, Cochlear Implant, Regenration, Cell Therapy; Biomedical Engineering; Engineering

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APA (6^th Edition):

Ramamurthy, P. (2012). Mouse Embryonic Stem Cells Differentiated into Neuron-like Cells or Schwann Cell-like Cells for the Development of Strategies to Ameliorate Deafness. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/94096

Chicago Manual of Style (16^th Edition):

Ramamurthy, Poornapriya. “Mouse Embryonic Stem Cells Differentiated into Neuron-like Cells or Schwann Cell-like Cells for the Development of Strategies to Ameliorate Deafness.” 2012. Doctoral Dissertation, University of Michigan. Accessed March 04, 2021. http://hdl.handle.net/2027.42/94096.

MLA Handbook (7^th Edition):

Ramamurthy, Poornapriya. “Mouse Embryonic Stem Cells Differentiated into Neuron-like Cells or Schwann Cell-like Cells for the Development of Strategies to Ameliorate Deafness.” 2012. Web. 04 Mar 2021.

Vancouver:

Ramamurthy P. Mouse Embryonic Stem Cells Differentiated into Neuron-like Cells or Schwann Cell-like Cells for the Development of Strategies to Ameliorate Deafness. [Internet] [Doctoral dissertation]. University of Michigan; 2012. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/2027.42/94096.

Council of Science Editors:

Ramamurthy P. Mouse Embryonic Stem Cells Differentiated into Neuron-like Cells or Schwann Cell-like Cells for the Development of Strategies to Ameliorate Deafness. [Doctoral Dissertation]. University of Michigan; 2012. Available from: http://hdl.handle.net/2027.42/94096

24. Shiraishi, Atsushi. Generation of thalamic neurons from mouse embryonic stem cells : マウス胚性幹細胞からの視床神経の分化誘導.

Degree: 博士(医学), 2018, Kyoto University / 京都大学

URL: http://hdl.handle.net/2433/230993 ; http://dx.doi.org/10.14989/doctor.k20790

新制・課程博士

甲第20790号

医博第4290号

Subjects/Keywords: mouse ES cells; thalamus; caudal forebrain; self-organization; SFEBq

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APA (6^th Edition):

Shiraishi, A. (2018). Generation of thalamic neurons from mouse embryonic stem cells : マウス胚性幹細胞からの視床神経の分化誘導. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/230993 ; http://dx.doi.org/10.14989/doctor.k20790

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shiraishi, Atsushi. “Generation of thalamic neurons from mouse embryonic stem cells : マウス胚性幹細胞からの視床神経の分化誘導.” 2018. Thesis, Kyoto University / 京都大学. Accessed March 04, 2021. http://hdl.handle.net/2433/230993 ; http://dx.doi.org/10.14989/doctor.k20790.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shiraishi, Atsushi. “Generation of thalamic neurons from mouse embryonic stem cells : マウス胚性幹細胞からの視床神経の分化誘導.” 2018. Web. 04 Mar 2021.

Vancouver:

Shiraishi A. Generation of thalamic neurons from mouse embryonic stem cells : マウス胚性幹細胞からの視床神経の分化誘導. [Internet] [Thesis]. Kyoto University / 京都大学; 2018. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/2433/230993 ; http://dx.doi.org/10.14989/doctor.k20790.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shiraishi A. Generation of thalamic neurons from mouse embryonic stem cells : マウス胚性幹細胞からの視床神経の分化誘導. [Thesis]. Kyoto University / 京都大学; 2018. Available from: http://hdl.handle.net/2433/230993 ; http://dx.doi.org/10.14989/doctor.k20790

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. ZHANG WEIWEI. Regulation of gene expression by esrrb in embryonic stem cells.

Degree: 2008, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/28317

Subjects/Keywords: ES cells; self-renewal; pluripotency; differentiation; Nanog; Esrrb

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APA (6^th Edition):

WEIWEI, Z. (2008). Regulation of gene expression by esrrb in embryonic stem cells. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/28317

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

WEIWEI, ZHANG. “Regulation of gene expression by esrrb in embryonic stem cells.” 2008. Thesis, National University of Singapore. Accessed March 04, 2021. http://scholarbank.nus.edu.sg/handle/10635/28317.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

WEIWEI, ZHANG. “Regulation of gene expression by esrrb in embryonic stem cells.” 2008. Web. 04 Mar 2021.

Vancouver:

WEIWEI Z. Regulation of gene expression by esrrb in embryonic stem cells. [Internet] [Thesis]. National University of Singapore; 2008. [cited 2021 Mar 04]. Available from: http://scholarbank.nus.edu.sg/handle/10635/28317.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

WEIWEI Z. Regulation of gene expression by esrrb in embryonic stem cells. [Thesis]. National University of Singapore; 2008. Available from: http://scholarbank.nus.edu.sg/handle/10635/28317

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lund

26. Miskinyte, Giedre. Generation of cortical neurons through reprogramming technology.

Degree: 2018, University of Lund

URL: https://lup.lub.lu.se/record/d960f763-7d64-44c6-9574-c623790a429d ; https://portal.research.lu.se/ws/files/54878264/Kvist_kappa_G5_hela_avh.pdf

► The human cortex is affected by several debilitating acute and chronic neurodegenerative disorders such as stroke, traumatic brain injury, amyotrophic lateral sclerosis and Alzheimer’s disease,… (more)

▼ The human cortex is affected by several debilitating acute and chronic neurodegenerative disorders such as stroke, traumatic brain injury, amyotrophic lateral sclerosis and Alzheimer’s disease, which target specific types of cortical neurons. Emerging evidence indicates that stem cells and reprogrammed cells can be used to generate human cortical neurons both for cell replacement by transplantation, and for disease modeling and drug screening. Several laboratories have established in vitro protocols for the derivation of excitatory pyramidal neurons, the principal type of neuron in the adult cortex, from human pluripotent stem cells (hPSCs). Efficient production of corticofugal projection neurons (CfuPNs) from ES cells has also been reported. Alternatively, human fibroblasts have been directly converted into induced neuronal (iN) cells. However, cortical neurons have not been produced by this method. Also, it remains to be assessed how closely the derived cortical neurons resemble their in vivo counterparts, as well as, whether the generated cells are capable of integrating into human neuronal circuits.The work presented in this thesis demonstrates that cortical neuronal cells can be produced from different types of starting cells: human fibroblasts, human ES cells and human iPSC-derived NSPCs. Regardless the starting cell context and derivation protocol, we have produced in all studies neuronal cells pyramidal in shape, expressing key cortical markers and functional in vitro. More importantly, we showed that these cells are capable of forming synaptic connections with adult human cortical neurons. It remains to be assessed whether cells derived in this thesis are capable of projecting to correct brain regions in vivo. Nonetheless, by providing the first evidence that cortical neurons derived here integrate in adult host neural networks also in a human-to-human grafting situation, this thesis represents an early but important step in the clinical translation of neuronal replacement to promote recovery in the injured brain.

Subjects/Keywords: Neurosciences; Neurology; Cortex; Reprogramming; human ES cells; transcription factor programming; cortical projection neurons; Human adult cortical slices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miskinyte, G. (2018). Generation of cortical neurons through reprogramming technology. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/d960f763-7d64-44c6-9574-c623790a429d ; https://portal.research.lu.se/ws/files/54878264/Kvist_kappa_G5_hela_avh.pdf

Chicago Manual of Style (16^th Edition):

Miskinyte, Giedre. “Generation of cortical neurons through reprogramming technology.” 2018. Doctoral Dissertation, University of Lund. Accessed March 04, 2021. https://lup.lub.lu.se/record/d960f763-7d64-44c6-9574-c623790a429d ; https://portal.research.lu.se/ws/files/54878264/Kvist_kappa_G5_hela_avh.pdf.

MLA Handbook (7^th Edition):

Miskinyte, Giedre. “Generation of cortical neurons through reprogramming technology.” 2018. Web. 04 Mar 2021.

Vancouver:

Miskinyte G. Generation of cortical neurons through reprogramming technology. [Internet] [Doctoral dissertation]. University of Lund; 2018. [cited 2021 Mar 04]. Available from: https://lup.lub.lu.se/record/d960f763-7d64-44c6-9574-c623790a429d ; https://portal.research.lu.se/ws/files/54878264/Kvist_kappa_G5_hela_avh.pdf.

Council of Science Editors:

Miskinyte G. Generation of cortical neurons through reprogramming technology. [Doctoral Dissertation]. University of Lund; 2018. Available from: https://lup.lub.lu.se/record/d960f763-7d64-44c6-9574-c623790a429d ; https://portal.research.lu.se/ws/files/54878264/Kvist_kappa_G5_hela_avh.pdf

Kyoto University

27. Shiraishi, Atsushi. Generation of thalamic neurons from mouse embryonic stem cells .

Degree: 2018, Kyoto University

URL: http://hdl.handle.net/2433/230993

Subjects/Keywords: mouse ES cells; thalamus; caudal forebrain; self-organization; SFEBq

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shiraishi, A. (2018). Generation of thalamic neurons from mouse embryonic stem cells . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/230993

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shiraishi, Atsushi. “Generation of thalamic neurons from mouse embryonic stem cells .” 2018. Thesis, Kyoto University. Accessed March 04, 2021. http://hdl.handle.net/2433/230993.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shiraishi, Atsushi. “Generation of thalamic neurons from mouse embryonic stem cells .” 2018. Web. 04 Mar 2021.

Vancouver:

Shiraishi A. Generation of thalamic neurons from mouse embryonic stem cells . [Internet] [Thesis]. Kyoto University; 2018. [cited 2021 Mar 04]. Available from: http://hdl.handle.net/2433/230993.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shiraishi A. Generation of thalamic neurons from mouse embryonic stem cells . [Thesis]. Kyoto University; 2018. Available from: http://hdl.handle.net/2433/230993

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Schnetz, Michael Paul. Investigation of the Molecular Function of CHD7, the Protein Implicated in CHARGE Syndrome, Using Next-Generation Genomics.

Degree: PhD, Genetics, 2010, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1275581022

► Eukaryotes use chromatin to package large, complex genomes into the nucleus of their cells. As such, chromatin structure plays a key role in virtually all… (more)

▼ Eukaryotes use chromatin to package large, complex genomes into the nucleus of their cells. As such, chromatin structure plays a key role in virtually all DNA-centric processes including gene transcription. Specifically, chromatin packaging and specific histone chemical modifications function to regulate gene expression. The chromodomain helicase DNA-binding (CHD) protein family is a class of chromatin remodeling enzymes that play roles in these processes. Despite what is known about the CHD protein family, the molecular function of CHD7 has not been characterized. CHD7 is required for proper mammalian development as mutations in CHD7 result in a developmental disease known as CHARGE syndrome in which multiple organ systems are affected including the eyes, heart, and genitals. Using a genomics approach, the molecular function of CHD7 was investigated. CHD7 was found to bind chromatin largely outside of gene transcriptional start sites in an overall pattern that was cell-specific. CHD7 binding correlates with cell-specific gene expression and harbors features of gene enhancer elements, namely H3K4me1 and DNaseI hypersensitivity. CHD7, to a less extent, is found also at gene promoters marked with H3K4me3. CHD7 binds H3K4me via its chromodomains. In mouse ES cells, CHD7 binding tracks with H3K4me1 methylation during cellular differentiation and colocalizes with ES-specific transcription factors including Oct4, Nanog, and Sox2. CHD7 binding sites represent functional enhancers as tested in luciferase reporter assays. Finally, loss of CHD7 results in both expression increases as well as decreases, however, CHD7 binding correlates with increased gene expression. Collectively, these studies indicate that CHD7 functions to regulate cell-specific gene transcription through enhancer interactions by limiting the expression of its target genes. The function of CHD7 uncovers a novel regulatory mechanism at gene enhancers that suggests the overall regulatory output of any enhancer is the combination of both positive and negative-acting factors. Advisors/Committee Members: Scacheri, Peter (Advisor).

Subjects/Keywords: Genetics; CHD7; CHARGE Syndrome; Chromatin Remodeling; mouse ES cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schnetz, M. P. (2010). Investigation of the Molecular Function of CHD7, the Protein Implicated in CHARGE Syndrome, Using Next-Generation Genomics. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1275581022

Chicago Manual of Style (16^th Edition):

Schnetz, Michael Paul. “Investigation of the Molecular Function of CHD7, the Protein Implicated in CHARGE Syndrome, Using Next-Generation Genomics.” 2010. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed March 04, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1275581022.

MLA Handbook (7^th Edition):

Schnetz, Michael Paul. “Investigation of the Molecular Function of CHD7, the Protein Implicated in CHARGE Syndrome, Using Next-Generation Genomics.” 2010. Web. 04 Mar 2021.

Vancouver:

Schnetz MP. Investigation of the Molecular Function of CHD7, the Protein Implicated in CHARGE Syndrome, Using Next-Generation Genomics. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 2010. [cited 2021 Mar 04]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1275581022.

Council of Science Editors:

Schnetz MP. Investigation of the Molecular Function of CHD7, the Protein Implicated in CHARGE Syndrome, Using Next-Generation Genomics. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1275581022

29. LAI ZHENYANG. Transcriptome Study of Human Embryonic Stem Cells and Knockdown Study of a Pluripotency Marker, LIN28.

Degree: 2010, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/20462

Subjects/Keywords: human ES cells; pluripotency; LIN28; RNAi; post-transcriptional regulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ZHENYANG, L. (2010). Transcriptome Study of Human Embryonic Stem Cells and Knockdown Study of a Pluripotency Marker, LIN28. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/20462

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ZHENYANG, LAI. “Transcriptome Study of Human Embryonic Stem Cells and Knockdown Study of a Pluripotency Marker, LIN28.” 2010. Thesis, National University of Singapore. Accessed March 04, 2021. https://scholarbank.nus.edu.sg/handle/10635/20462.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ZHENYANG, LAI. “Transcriptome Study of Human Embryonic Stem Cells and Knockdown Study of a Pluripotency Marker, LIN28.” 2010. Web. 04 Mar 2021.

Vancouver:

ZHENYANG L. Transcriptome Study of Human Embryonic Stem Cells and Knockdown Study of a Pluripotency Marker, LIN28. [Internet] [Thesis]. National University of Singapore; 2010. [cited 2021 Mar 04]. Available from: https://scholarbank.nus.edu.sg/handle/10635/20462.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ZHENYANG L. Transcriptome Study of Human Embryonic Stem Cells and Knockdown Study of a Pluripotency Marker, LIN28. [Thesis]. National University of Singapore; 2010. Available from: https://scholarbank.nus.edu.sg/handle/10635/20462

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. LIM KEAT SANDY. Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency.

Degree: 2008, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/28150

Subjects/Keywords: ES cells; Lefty; Nodal signaling; Morphogen; self renewal; pluripotency

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SANDY, L. K. (2008). Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/28150

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SANDY, LIM KEAT. “Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency.” 2008. Thesis, National University of Singapore. Accessed March 04, 2021. https://scholarbank.nus.edu.sg/handle/10635/28150.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SANDY, LIM KEAT. “Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency.” 2008. Web. 04 Mar 2021.

Vancouver:

SANDY LK. Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency. [Internet] [Thesis]. National University of Singapore; 2008. [cited 2021 Mar 04]. Available from: https://scholarbank.nus.edu.sg/handle/10635/28150.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SANDY LK. Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency. [Thesis]. National University of Singapore; 2008. Available from: https://scholarbank.nus.edu.sg/handle/10635/28150

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations