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University of Helsinki
1.
Hänninen, Oona.
Feline corneal sequestra – Morphological characterisation of normal and sequestered feline cornea.
Degree: Department of Veterinary Biosciences; Helsingin yliopisto, Eläinlääketieteellinen tiedekunta, Eläinlääketieteellisten biotieteiden osasto; Helsingfors universitet, Veterinärmedicinska fakulteten, Avdelningen för veterinärmedicinska biovetenskap, 2014, University of Helsinki
URL: http://hdl.handle.net/10138/135623 
► Kissan normaalin sarveiskalvon ja kahden sarveiskalvon sekvesterin rakennetta kartoitettiin valo- ja läpäisyelektronimikroskopian avulla. Uudempaa tekniikkaa, näytepinnan automatisoitua pyyhkäisyelektronimikroskooppista sarjakuvantamista hyödynnettiin kissan sarveiskalvon levyepiteelisolun ja sarveiskalvon…
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▼ Kissan normaalin sarveiskalvon ja kahden sarveiskalvon sekvesterin rakennetta kartoitettiin valo- ja läpäisyelektronimikroskopian avulla. Uudempaa tekniikkaa, näytepinnan automatisoitua pyyhkäisyelektronimikroskooppista sarjakuvantamista hyödynnettiin kissan sarveiskalvon levyepiteelisolun ja sarveiskalvon pinnan kolmiulotteiseen mallintamiseen.
Kissan sarveiskalvosekvesterit ovat rajattuja, pigmentoituja kuoliokappaleita sarveiskalvon stroomassa. Aikaisemmista tutkimuksista huolimatta sekvesterin etiologiaa, patogeneesiä tai pigmentin aiheuttajaa ei ole pystytty selvittämään. Läpäisyelektronimikroskooppiset tutkimukset eivät ole riittäneet pigmentin tunnistamiseen tai strooman solujen, keratosyyttien, roolin selvittämiseen sekvesterin taudinkuvassa. Sekvestereitä ei ole aikaisemmin tutkittu kolmiulotteisilla elektronimikroskooppisilla kuvantamismenetelmillä, joten tässä tutkimuksessa aloitettiin työ kehittämällä sopiva näytteenvalmistusmenetelmä sarveiskalvon tutkimiseksi.
Kontrollinäytteen valo- ja elektronimikroskooppisten tutkimusten tulokset vastasivat nykykäsitystä kissan sarveiskalvon normaalianatomiasta. Strooman kollageenisälekerrokset olivat säännöllisiä, ja normaalit keratosyytit sisälsivät runsaasti karkeaa solulimakalvostoa, mikä liittyy kollageenisäikeiden tuottamiseen. Lievästä sekvesterinäyteestä löytyi liuskoittuneita kollageenisäleitä ja apoptoottisia tai nekroottisia keratosyyttejä. Pahalaatuisessa sekvesterinäytteessä havaittiin strooman normaalin kaltainen säännöllinen rakenne, ehjiä keratosyyttejä, ja runsas määrä elektroneja läpäiseviä profiileja, joita ei havaittu muissa näytteissä. Näissä rakenteissa on voinut olla pigmenttirakkuloita, jotka ovat huuhtoutuneet näytteenvalmistuksessa pois.
Kissan sarveiskalvon epiteelisolujen kolmiulotteinen analyysi osoitti pieniä sormimaisia ulokkeita joilla vierekkäiset solut kiinnittyvät toisiinsa. Epiteelisolun tuma oli kiekkomainen, sillä oli litteä pinta ja ulospäin kaareva pohja. Kolme erilaista pinnallisen levyepiteelisolun pintarakennemallia tunnistettiin: sormimaisia rakenteita, labyrinttimainen verkosto poimuja ja näiden yhdistelmä.
Tutkimuksessa onnistuttiin kehittämään sopiva menetelmä sarveiskalvonäytteen valmistamiseksi kolmiulotteista sarjakuvantamista varten. Tutkimusta voidaan jatkaa sarveiskalvon strooman ja keratosyyttien kuvantamiseksi. Lisänäytteet mahdollistaisivat kissan normaalin sarveiskalvon ja sekvesterin keratosyyttien vertailun. Erot keratosyyttien rakenteessa osoittaisivat, että soluilla voi olla osuus sarveiskalvon sekvestereiden muodostumisessa.
Subjects/Keywords: cat; cornea; corneal sequestrum; corneal epithelium; light microscopy; Anatomia; Anatomi; Anatomy; cat; cornea; corneal sequestrum; corneal epithelium; light microscopy
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APA (6th Edition):
Hänninen, O. (2014). Feline corneal sequestra – Morphological characterisation of normal and sequestered feline cornea. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/135623
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hänninen, Oona. “Feline corneal sequestra – Morphological characterisation of normal and sequestered feline cornea.” 2014. Thesis, University of Helsinki. Accessed January 18, 2021.
http://hdl.handle.net/10138/135623.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hänninen, Oona. “Feline corneal sequestra – Morphological characterisation of normal and sequestered feline cornea.” 2014. Web. 18 Jan 2021.
Vancouver:
Hänninen O. Feline corneal sequestra – Morphological characterisation of normal and sequestered feline cornea. [Internet] [Thesis]. University of Helsinki; 2014. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10138/135623.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hänninen O. Feline corneal sequestra – Morphological characterisation of normal and sequestered feline cornea. [Thesis]. University of Helsinki; 2014. Available from: http://hdl.handle.net/10138/135623
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
2.
Kalangara, Jerry P.
Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas.
Degree: 2011, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/929
► OBJECTIVE: To develop an animal model for the implementation of human telomerase enzyme reverse transcriptase (hTERT)-immortalized human corneal epithelial cell line (hTCEpi) transduced with the…
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▼ OBJECTIVE: To develop an animal model for the implementation of human telomerase enzyme reverse transcriptase (hTERT)-immortalized human
corneal epithelial cell line (hTCEpi) transduced with the hygromycin-thymidine kinase gene (HyTK) as a viable cell source for the reconstruction of the
corneal surface.
METHODS: HyTK cells were cultured in KGM-2 serum-free culture media under hygromycin B selection. Limbal stem cell deficiency (LSCD) was established in athymic nude mice (n=75) using ethylenediaminetetraacetic acid (EDTA) and mitomycin C treatment followed by mechanical debridement of
corneal and limbal
epithelium. Immunofluorescence (IF) using Anti-Laminin and Propidium iodide (PI) was used to assess presence of basement membrane after epithelial removal. Cultured HyTK cells were stained with Cell Tracker Green CMFDA (5-chloromethylfluorescein diacetate) and transplanted onto the right eye after epithelial removal; the left eye served as a control. Transplanted cells were evaluated at 4 hours, 1 day, and 7 days post-transplantation using laser scanning confocal microscopy (LSCM). At 4 hours and 1 day, corneas were imaged for the presence of CMFDA. At day 7, corneas were stained using antibodies to Keratin 3, Ki-67, and the fluorescent probes – PI, and Phalloidin. Cytotoxicity of ganciclovir was assessed at concentrations of 0.1, 0.5, 1.0, 5.0, and 10.0 μM using Live-Dead Assay.
RESULTS: IF confirmed an intact
corneal basement membrane following epithelial removal. At 4 hours and 1 day post-transplantation, CMFDA staining demonstrated that transplanted cells were dispersed throughout the
corneal surface. After 7 days, HyTK cells showed stratification and IF confirmed a differentiated
corneal epithelial phenotype. Incubation in ganciclovir induced a cytotoxic effect on HyTK cells in vitro and had no significant effect on the hTCEpi control cell line. This effect was significant at 0.5 µM (p < 0.05).
CONCLUSIONS: These studies demonstrate the first reconstitution of a multi-layered, differentiated
corneal epithelium by HyTK cells in the nude mouse model; further, proliferating HyTK cells can be killed with ganciclovir treatment in vitro, which may reduce the potential for risk of oncogenic transformation in vivo.
Advisors/Committee Members: Cavanagh, H. Dwight.
Subjects/Keywords: Cornea; Corneal Transplantation; Epithelium, Corneal
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APA ·
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APA (6th Edition):
Kalangara, J. P. (2011). Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/929
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kalangara, Jerry P. “Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed January 18, 2021.
http://hdl.handle.net/2152.5/929.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kalangara, Jerry P. “Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas.” 2011. Web. 18 Jan 2021.
Vancouver:
Kalangara JP. Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/2152.5/929.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kalangara JP. Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/929
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
Ambrósio Júnior, Renato.
"Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial".
Degree: PhD, Oftalmologia, 2004, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/
;
► Objetivo: Verificar resposta após debridamento epitelial de córneas humanas. Métodos: Córneas normais foram submetidas a debridamento antes da cirurgia de enucleação. Realizou-se histologia, TUNEL, Ki67,…
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▼ Objetivo: Verificar resposta após debridamento epitelial de córneas humanas. Métodos: Córneas normais foram submetidas a debridamento antes da cirurgia de enucleação. Realizou-se histologia, TUNEL, Ki67, SMA e microscopia eletrônica. Resultados: Seis córneas foram debridadas e preservadas entre ½ e 65 horas, apresentando apoptose nos ceratócitos do estroma anterior. Células estromais em proliferação foram observadas apenas no tempo de 65 horas. Miofibroblastos não foram encontrados. Uma córnea serviu de controle. Conclusões: Os eventos observados em córneas humanas após debridamento epitelial, apoptose e proliferação dos ceratócitos, foram semelhantes aos descritos em animais de experimentação
Purpose: To examine the early wound healing response to epithelial scrape in human corneas. Methods: Normal corneas had epithelial scrape prior to enucleation. Histology, TUNEL assay, Ki67, SMA and transmission electron microscopy were performed. Results: Epithelial scrape was performed in six corneas from ½ to 65 hours prior to preservation. Keratocyte apoptosis was detected in the anterior stroma in all scraped corneas. Keratocyte proliferation was detected exclusively 65 hours after scrape. No myofibroblast was detected. One cornea was not scraped (control). Conclusion: Results obtained in human corneas (keratocyte apoptosis and proliferation) were similar to animal models
Advisors/Committee Members: Alves, Milton Ruiz, Jose, Newton Kara.
Subjects/Keywords: APOPTOSE; APOPTOSIS; CORNEAL STROMA/anatomy & histology; CORNEAL STROMA/cytology; CORNEAL STROMA/physiology; EPITÉLIO DA CÓRNEA/anatomia & histologia; EPITÉLIO DA CÓRNEA/cirurgia; EPITÉLIO DA CÓRNEA/crescimento & desenvolvimento; EPITHELIUM CORNEAL/anatomy & histology; EPITHELIUM CORNEAL/growth & development; EPITHELIUM CORNEAL/surgery; ESTROMA CORNEAL/anatomia & histologia; ESTROMA CORNEAL/citologia; ESTROMA CORNEAL/fisiopatologia; IMMUNOHISTOCHEMISTRY/methods; IMUNOHISTOQUÍMICA/métodos; MICROSCOPIA CONFOCAL/métodos; MICROSCOPIA DE FLUORESCÊNCIA/métodos; MICROSCOPIA ELETRÔNICA/métodos; MICROSCOPY CONFOCAL/methods; MICROSCOPY ELECTRON/methods; MICROSCOPY FLUORESCENCE/methods; MITOSE/fisiologia; MITOSIS/physiology
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Ambrósio Júnior, R. (2004). "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial". (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;
Chicago Manual of Style (16th Edition):
Ambrósio Júnior, Renato. “"Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial".” 2004. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;.
MLA Handbook (7th Edition):
Ambrósio Júnior, Renato. “"Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial".” 2004. Web. 18 Jan 2021.
Vancouver:
Ambrósio Júnior R. "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial". [Internet] [Doctoral dissertation]. University of São Paulo; 2004. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;.
Council of Science Editors:
Ambrósio Júnior R. "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial". [Doctoral Dissertation]. University of São Paulo; 2004. Available from: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;
University of Hong Kong
4.
Tipoe, George Lim.
A histological and
ultrastructural morphometric assessment of malignant potential in
human colorectal epithelium.
Degree: 1993, University of Hong Kong
URL: http://hdl.handle.net/10722/34592
Subjects/Keywords: Epithelium - Histology.; Colon
(Anatomy) - Cancer.;
Epithelium - Tumors.;
Epithelium - Ultrastructure.
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APA ·
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APA (6th Edition):
Tipoe, G. L. (1993). A histological and
ultrastructural morphometric assessment of malignant potential in
human colorectal epithelium. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/34592
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tipoe, George Lim. “A histological and
ultrastructural morphometric assessment of malignant potential in
human colorectal epithelium.” 1993. Thesis, University of Hong Kong. Accessed January 18, 2021.
http://hdl.handle.net/10722/34592.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tipoe, George Lim. “A histological and
ultrastructural morphometric assessment of malignant potential in
human colorectal epithelium.” 1993. Web. 18 Jan 2021.
Vancouver:
Tipoe GL. A histological and
ultrastructural morphometric assessment of malignant potential in
human colorectal epithelium. [Internet] [Thesis]. University of Hong Kong; 1993. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10722/34592.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tipoe GL. A histological and
ultrastructural morphometric assessment of malignant potential in
human colorectal epithelium. [Thesis]. University of Hong Kong; 1993. Available from: http://hdl.handle.net/10722/34592
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cape Town
5.
Chou, Kuang-Yi.
Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium.
Degree: Image, Division of Cell Biology, 1994, University of Cape Town
URL: http://hdl.handle.net/11427/27148
► This project examined the role of the cytoskeleton in regulatory mechanisms of the amiloride-sensitive Na⁺ channels in isolated frog skin epithelium. The epithelium from ventral…
(more)
▼ This project examined the role of the cytoskeleton in regulatory mechanisms of the amiloride-sensitive Na⁺ channels in isolated frog skin
epithelium. The
epithelium from ventral frog skin is a model tissue which has proved significant in our understanding of the basic principles involved in water and Na⁺ homeostasis. In particular, this project examines ways in which local (non-hormonal) and hormonal regulatory mechanisms adjust the Na⁺ permeability of apical membranes of frog skin
epithelium. Both mechanisms contain factors that are known to increase the apical membrane Na⁺ permeability mainly by increases in the number of open channels. The origin of these new open channels is unknown but, it is postulated that they could arise either by activation of quiescent channels already present in the apical membrane, or by recruitment of channels from cytoplasmic stores. Regarding the latter hypothesis, we also examined the idea that the cytoskeleton might somehow be involved in the insertion of Na⁺ channels within vesicles, into the apical membrane. This is based on the fact that the cytoskeleton is involved in a similar mechanism whereby, in the toad urinary bladder, anti-diuretic hormone (ADH) causes the insertion of aggregates with water channels. Much current interest focuses on the role of the cytoskeleton in the regulation of epithelial Na⁺ channels. To test this hypothesis, we used noise analysis to examine the effects of disrupting the cytoskeleton, on two different mechanisms which bring about changes in open channel densities. The mechanisms are: (1) lowering mucosal Na⁺ concentration (non-hormonal), and (2) addition of arginine-vasopressin (A VP) (hormonal). Non-hormonal, autoregulatory changes in apical membrane Na⁺ conductance were examined by investigating the effects of reducing the mucosal Na⁺ concentration. Our results showed that lowering the mucosal Na⁺ concentration induced large increases in the open channel density in order to stabilise the transport rate. In addition, we observed an average 55-60% increase in the open channel probability, which implies that in
epithelium from Rana fuscigula, changes of channel open probability are also an important mechanism in the autoregulation of channel densities in response to a reduction in mucosal Na⁺. The hormonal control of Na⁺ channels by A VP has been intensively studied by noise analysis and the patch clamp. Our results confirmed previous reports that A VP increases the Na⁺ transport rate by increasing the number of open Na⁺ channels, primarily through large changes in the total number of channels, without a significant change in open probability. Regarding the role of the cytoskeleton in regulation of Na⁺ channels and/or its possible role in control of inserting putative vesicles with Na⁺ channels, we studied the effects of disrupting the cytoskeleton on the two regulatory mechanisms. Disrupting microtubules with colchicine had no, or very little effect on either of the regulatory mechanisms. On the other hand, the integrity of the microfilaments was…
Advisors/Committee Members: Els, W J (advisor).
Subjects/Keywords: Epithelium - anatomy and histology; Cytoskeleton - physiology; Skin - Anatomy and histology; Anura; Sodium channels
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APA (6th Edition):
Chou, K. (1994). Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/27148
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chou, Kuang-Yi. “Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium.” 1994. Thesis, University of Cape Town. Accessed January 18, 2021.
http://hdl.handle.net/11427/27148.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chou, Kuang-Yi. “Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium.” 1994. Web. 18 Jan 2021.
Vancouver:
Chou K. Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium. [Internet] [Thesis]. University of Cape Town; 1994. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/11427/27148.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chou K. Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium. [Thesis]. University of Cape Town; 1994. Available from: http://hdl.handle.net/11427/27148
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of New South Wales
6.
Bandamwar, Kalika.
Mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea.
Degree: Optometry & Vision Science, 2011, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/52429
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11102/SOURCE01?view=true
► It is for well over a century that fluorescein has been used as a preferred dye for identifying and enhancing corneal surface change. Superficial micropunctate…
(more)
▼ It is for well over a century that fluorescein has been used as a preferred dye for identifying and enhancing
corneal surface change. Superficial micropunctate staining of the
corneal epithelium is the most commonly encountered clinical manifestation of fluorescein staining. Despite its well accepted use, neither the cellular basis nor the clinical significance of this regularly seen phenomenon are yet clear. Despite this uncertainty, the presence of
corneal staining has traditionally been used as a clinical tool to identify
corneal surface changes. Recent literature reports have argued for more thorough scientific evidence to be produced attesting to the exact status of the
corneal epithelium when it appears to have stained with fluorescein.In this thesis we systematically evaluated the mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea. A series of experiments were designed to determine the cellular basis of
corneal staining. Six mechanisms were identified from literature reports, these were pooling of fluorescein in voids on the ocular surface, accumulation of dye solution in intercellular spaces, staining of live, damaged or dead cells, a response to exposed cells un-protected by the mucin layer and the possibility that the staining appearance is an artefact. Evaluations were aimed to determine which of these mechanisms dominates in the process.A staining model was developed to facilitate evaluation in human subjects and when this could not be used due to safety or ethical considerations, in vitro cell culture and ex vivo organ culture models were utilized. Observations were performed at high magnification and were aided by simultaneous staining with metabolic dyes to identify live, apoptotic and dead cells. The results of these studies suggest that healthy
corneal epithelial cells take up fluorescein to a moderate level and dead cells stain minimally. Fluorescent intensities associated with both these situations are not typically observed with clinical slit-lamp bio-microscopy. Irrespective of the cause of insult, apoptotic cells were associated with bright florescence, i.e. hyper-fluorescence relative to the background of normal cells. We conclude that the bright fluorescence perceived as micropunctate dots on ocular surface when observed with slit-lamp bio-microscope indicates apoptotic, but not dead, cells. Such staining was also associated with an increase in the level of some, but not all inflammatory mediators in the tear film.
Advisors/Committee Members: Papas, Eric, Optometry & Vision Science, Faculty of Science, UNSW, Garrett, Qian, Optometry & Vision Science, Faculty of Science, UNSW.
Subjects/Keywords: Fluorescein; Corneal staining; Corneal epithelium; Contact lens; Inflammation; Apoptosis
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APA ·
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APA (6th Edition):
Bandamwar, K. (2011). Mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52429 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11102/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Bandamwar, Kalika. “Mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea.” 2011. Doctoral Dissertation, University of New South Wales. Accessed January 18, 2021.
http://handle.unsw.edu.au/1959.4/52429 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11102/SOURCE01?view=true.
MLA Handbook (7th Edition):
Bandamwar, Kalika. “Mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea.” 2011. Web. 18 Jan 2021.
Vancouver:
Bandamwar K. Mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea. [Internet] [Doctoral dissertation]. University of New South Wales; 2011. [cited 2021 Jan 18].
Available from: http://handle.unsw.edu.au/1959.4/52429 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11102/SOURCE01?view=true.
Council of Science Editors:
Bandamwar K. Mechanism and clinical significance of superficial micropunctate fluorescein staining of the cornea. [Doctoral Dissertation]. University of New South Wales; 2011. Available from: http://handle.unsw.edu.au/1959.4/52429 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11102/SOURCE01?view=true
University of British Columbia
7.
Adams, Anne Theresa.
The rat ovarian surface epithelium in vitro.
Degree: PhD, Zoology, 1982, University of British Columbia
URL: http://hdl.handle.net/2429/23293
► The ovarian surface epithelium, a very small portion of the total mass of the ovary, is generally thought to be the site of origin of…
(more)
▼ The ovarian surface epithelium, a very small portion of the total mass of the ovary, is generally thought to be the site of origin of over 85% of ovarian cancers. Such cancers are classified with the "Common Epithelial Tumours" of the ovary. In most industrialized countries, malignancies of the ovary rank fourth in cancer deaths in women; over 70% of these neoplasms have spread beyond the ovary when first diagnosed.
Experimental approaches to the study of carcinogenesis in this tissue have been limited by the lack of pure populations of ovarian surface epithelial cells. Studies done on rodents in vivo suggest that both chemicals and C-type RNA viruses can induce ovarian cancers similar to those which are said to arise from the surface epithelium. However, the cell of origin cannot be proven in such studies.
The purpose of this project was to develop a model for ovarian cancers of surface epithelial origin based on-carcinogenesis in vitro. To this end a method was devised to culture the rat ovarian surface epithelium in pure form. These cultured cells, whose identity has been confirmed by morphological, histochemical and ultrastructural means, are polygonal with clear cytoplasm, have well-defined borders, and grow in confluent monolayers. Their morphology is quite distinct from those of other ovarian cells in vitro. Cultured rat ovarian surface epithelial cells are histochemically positive for 17β-hydroxysteroid dehydrogenase, and negative for
Δ5-3β-hydroxysteroid dehydrogenase, the same as in frozen sections of whole rat ovary. Ultrastructurally, cultured surface epithelial cells have basal laminae, microvilli, apical intercellular junctions, large nuclei, abundant rough endoplasmic reticulum, Golgi complexes, perinuclear bundles of microfilaments, and numerous vesicles.
Although the ovarian suface epithelium is suspected of being an estrogen target tissue, there is no previous report of estrogen receptors in these cells. In this study cultured rat ovarian surface epithelial cells have been shown by autoradiographic means to exhibit estrogen receptor-like activity. Translocation of tritiated estradiol from cytoplasm to nucleus, and estrogen-specific binding have been demonstrated. Estradiol was shown to be mitogenic for cultured ovarian surface epithelial cells. From these results, the surface epithelial .cells of the ovary should be considered an estrogen target tissue.
Kirsten murine sarcoma virus was used to produce three transformed cell lines from pure, first passage cultures of these cells. These three lines retained 170-hydroxysteroid dehydrogenase activity and showed slight Δ5-3β-hydroxysteroid dehydrogenase activity. Tumours resulting when these cells were injected into immunosuppressed female rats were highly malignant and resembled histologically human endometrioid stromal sarcomas of the ovary. This neoplasm is classed with the "Common Epithelial Tumours" of the ovary, but is generally not
considered a derivative of the surface epithelium. In light of this study, perhaps this tumour should: be…
Subjects/Keywords: Rats – Anatomy; Ovaries – Cancer; Epithelium; Ovarian neoplasms; Rats – anatomy & histology
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APA (6th Edition):
Adams, A. T. (1982). The rat ovarian surface epithelium in vitro. (Doctoral Dissertation). University of British Columbia. Retrieved from http://hdl.handle.net/2429/23293
Chicago Manual of Style (16th Edition):
Adams, Anne Theresa. “The rat ovarian surface epithelium in vitro.” 1982. Doctoral Dissertation, University of British Columbia. Accessed January 18, 2021.
http://hdl.handle.net/2429/23293.
MLA Handbook (7th Edition):
Adams, Anne Theresa. “The rat ovarian surface epithelium in vitro.” 1982. Web. 18 Jan 2021.
Vancouver:
Adams AT. The rat ovarian surface epithelium in vitro. [Internet] [Doctoral dissertation]. University of British Columbia; 1982. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/2429/23293.
Council of Science Editors:
Adams AT. The rat ovarian surface epithelium in vitro. [Doctoral Dissertation]. University of British Columbia; 1982. Available from: http://hdl.handle.net/2429/23293
8.
Gross, Christelle.
Etude de l'implication des produits de glycation avancés et de leur récepteur RAGE dans la cicatrisation de l'épithélium cornéen : Advanced Glycation End Products and their Receptor RAGE in Human Corneal Epithelial Cells Wound Healing.
Degree: Docteur es, Physiologie et biologie des organismes - populations - interactions, 2018, Clermont Auvergne
URL: http://www.theses.fr/2018CLFAS010
► De par son rôle dans de nombreuses fonctions biologiques, RAGE est une récepteur membranaire crucial du développement embryonnaire jusqu’à l’âge adulte. Ce récepteur multi-ligand est…
(more)
▼ De par son rôle dans de nombreuses fonctions biologiques, RAGE est une récepteur membranaire crucial du développement embryonnaire jusqu’à l’âge adulte. Ce récepteur multi-ligand est capable d’activer de nombreuses cascades de signalisation, lui permettant entre autres d’être impliqué dans les processus inflammatoires et cicatriciels. Bien que décrite, son action cellulaire et moléculaire dans les processus de régénération/cicatrisation reste encore à éclaircir, malgré des études déjà menées sur les épithéliums cutanée et pulmonaire. Concernant la cicatrisation de l’épithélium cornéen, l’action de ce récepteur et de ses ligands est peu documentée et largement controversé. Ce travail a permis de tester l’effet de 2 ligands de RAGE (HMGB1 et AGEs) sur la cicatrisation de l’épithélium cornéen en utilisant un modèle in vitro de cellules épithéliales de cornée humaine (HCE). Il visait aussi à étudier les cascades de signalisation et les processus cellulaire mis en jeu suite à l’activation de ce récepteur. Les résultats obtenus ont tout d’abord permis de démontrer que l’action pro-cicatrisante du récepteur RAGE sur des cellules de l’épithélium cornéen, était ligand et dose spécifique. Ainsi seul le ligand AGEs promeut la cicatrisation indépendamment des processus de migration et de prolifération cellulaire. Dans cette étude le couple AGEs/RAGE est capable d’activer la cascade de signalisation NF-κB et la transcription d’un gène cible Connexine 43, dont le rôle a déjà été décrit durant la cicatrisation.Malgré la complexité du « signal RAGE », les premières pistes apportées par cette étude permettent d’envisager dans un futur proche la caractérisation précise de son action pro-cicatrisante au niveau de la sphère oculaire. Ceci passera non seulement par l’étude exhaustive des cascades de signalisations activées et des gènes régulés mais aussi par l’utilisation du modèle animal souris sauvage et RAGE -/-.
Because of its role in many biological functions, RAGE is a crucial transmembranous receptor from development to adulthood. This multiligand receptor can activate numerous signaling pathways, and it is involved in inflammatory and wound healing processes. Although already describe, molecular and cellular processes involved during wound healing still to be clarify despite some studies conducted on skin and lung epithelium. In the corneal epithelium wound healing, RAGE and its ligands effects still poorly understood and widely controversial. This work allowed the test of 2 ligands (HMGB1 and AGEs) on corneal epithelium healing using an in-vitro model of human corneal epithelial cells (HCE). It also aims to study the signaling pathways and cellular processes involved after this receptor activation. Results obtained permit to demonstrate a ligand and dose-dependent action of RAGE during this pro-healing process. Thus, only AGEs ligand promotes wound healing independently of cellular migration and proliferation processes. In this study, AGEs/RAGE couple can activate NF-kB signaling pathway and Connexin 43 target gene…
Advisors/Committee Members: Sapin, Vincent (thesis director).
Subjects/Keywords: Epithelium cornéen; Cicatrisation; AGEs; RAGE; Wound healing; Corneal epithelium; RAGE; AGEs; 617.7
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APA (6th Edition):
Gross, C. (2018). Etude de l'implication des produits de glycation avancés et de leur récepteur RAGE dans la cicatrisation de l'épithélium cornéen : Advanced Glycation End Products and their Receptor RAGE in Human Corneal Epithelial Cells Wound Healing. (Doctoral Dissertation). Clermont Auvergne. Retrieved from http://www.theses.fr/2018CLFAS010
Chicago Manual of Style (16th Edition):
Gross, Christelle. “Etude de l'implication des produits de glycation avancés et de leur récepteur RAGE dans la cicatrisation de l'épithélium cornéen : Advanced Glycation End Products and their Receptor RAGE in Human Corneal Epithelial Cells Wound Healing.” 2018. Doctoral Dissertation, Clermont Auvergne. Accessed January 18, 2021.
http://www.theses.fr/2018CLFAS010.
MLA Handbook (7th Edition):
Gross, Christelle. “Etude de l'implication des produits de glycation avancés et de leur récepteur RAGE dans la cicatrisation de l'épithélium cornéen : Advanced Glycation End Products and their Receptor RAGE in Human Corneal Epithelial Cells Wound Healing.” 2018. Web. 18 Jan 2021.
Vancouver:
Gross C. Etude de l'implication des produits de glycation avancés et de leur récepteur RAGE dans la cicatrisation de l'épithélium cornéen : Advanced Glycation End Products and their Receptor RAGE in Human Corneal Epithelial Cells Wound Healing. [Internet] [Doctoral dissertation]. Clermont Auvergne; 2018. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2018CLFAS010.
Council of Science Editors:
Gross C. Etude de l'implication des produits de glycation avancés et de leur récepteur RAGE dans la cicatrisation de l'épithélium cornéen : Advanced Glycation End Products and their Receptor RAGE in Human Corneal Epithelial Cells Wound Healing. [Doctoral Dissertation]. Clermont Auvergne; 2018. Available from: http://www.theses.fr/2018CLFAS010
University of Utah
9.
Dalley, Arthur Frederick.
The morphology of the canine (Beagle) liver;.
Degree: PhD, Neurobiology & Anatomy;, 1975, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1686/rec/1376
► In spite of an extremely large volume of literature concerning the microscopic and sub-microscopic morphology of the mammalian liver, most investigations have been carried out…
(more)
▼ In spite of an extremely large volume of literature concerning the microscopic and sub-microscopic morphology of the mammalian liver, most investigations have been carried out with only a few ‘representative’ species and there has been no definite statement of the nature of the canine liver. In the present study, the liver of the purebred Beagle was examined by means of light microscopy, graphic reconstruction, and corrosion cast techniques to: (1) test the validity of the ‘lobule’ concept of liver structure; (2) describe the relationships band patters of distribution displayed by the hepatic sinusoids and vasculature; (3) enable comparison of the canine liver with that of other species, especially those which have been used as models for mammalian liver structure; (4) establish the structural (architectural) principle of the liver. Serial histological sections were examined and an attempt was made to produce a plastic reconstruction of a liver lobule. However, the absence of anatomically-definable boundaries mad accurate reconstruction impossible. It was determined that the lobular pattern observed on section of liver tissue is an illusion resulting from blood pressure gradients and the regular interdigitation of the afferent and efferent vessels at uniform distances. This latter feature is clearly evident in the corrosion cast preparations. Graphic reconstructions of the channels for the hepatic vasculature support the theory that the attainment of the most regular interdigitation of the afferent and efferent vessels is the true principle of hepatic architecture. This concept was determined to be consistent with the functional aspect of maintaining uniform blood distribution in the hepatic parenchyma. The corrosion cast studies suggested the occurrence of arteriovenous anastomoses, but were inconclusive. Graphic reconstructions of the hepatic sinusoids from serial sections showed the sinusoids form a highly complex and anastomosing labyrinth which is continuous throughout the hepatic parenchyma. Electron microscopy studies of the ultrastructure of the perinsinusoidal space of Disse and the endothelial lining of hepatic sinusoids in several non-canine species have shown that specie differences do exist among mammals. In the present study, biopsy and necropsy specimens of Beagle livers examined via electron microscopy showed the endothelial lining of the hepatic sinusoids to be predominantly continuous. However, many openings occur in the lining, most of which are patent channels allowing direct communication between the sinusoidal lumen and the Disse space. Openings occur both as intercellular gaps and as intracellular fenestrations composing ‘sieve-plate’ formations in the cytoplasm of the endothelial cells. Diaphragms were occasionally observed occluding the intracellular fenestrations, but the majority is patent. A definitive basement membrane was not observed beneath the endothelial cells. The Disse space is almost completely occupied by the microvilli of the hepatic cells. Bundles of collagen fibrils are…
Subjects/Keywords: Anatomy; Histology
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APA (6th Edition):
Dalley, A. F. (1975). The morphology of the canine (Beagle) liver;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1686/rec/1376
Chicago Manual of Style (16th Edition):
Dalley, Arthur Frederick. “The morphology of the canine (Beagle) liver;.” 1975. Doctoral Dissertation, University of Utah. Accessed January 18, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1686/rec/1376.
MLA Handbook (7th Edition):
Dalley, Arthur Frederick. “The morphology of the canine (Beagle) liver;.” 1975. Web. 18 Jan 2021.
Vancouver:
Dalley AF. The morphology of the canine (Beagle) liver;. [Internet] [Doctoral dissertation]. University of Utah; 1975. [cited 2021 Jan 18].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1686/rec/1376.
Council of Science Editors:
Dalley AF. The morphology of the canine (Beagle) liver;. [Doctoral Dissertation]. University of Utah; 1975. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1686/rec/1376
University of California – Berkeley
10.
Augustin, Danielle Kristy.
Identifying Mechanism of Traversal of Corneal Epithelial Cells by Pseudomonas aerugnosa.
Degree: Microbiology, 2011, University of California – Berkeley
URL: http://www.escholarship.org/uc/item/3zc143tt
► Pseudomonas aeruginosa keratitis is a sight-threatening complication of contact lens wear. Ordinarily, the ocular surface is effective against microbial infiltration through a variety of mechanical,…
(more)
▼ Pseudomonas aeruginosa keratitis is a sight-threatening complication of contact lens wear. Ordinarily, the ocular surface is effective against microbial infiltration through a variety of mechanical, anatomical, and immunological defense mechanisms which protect the cornea. However, contact lens wear, ocular injury and/or surgery predispose individuals to bacterial keratitis. In the case of contact lens wear, the Gram-negative bacterium Pseudomonas aeruginosa is the most frequently isolated causative agent. As the number of antimicrobial compounds effective against P. aeruginosa decreases, due to of the acquisition and spread of antibiotic resistance, there is a growing need for novel therapeutic approaches corneal infection. The fact that P. aeruginosa can cross the corneal epithelium into the stroma to cause disease in contact lens wearers suggest a compromise in host defense. However, little is known of the mechanisms by which bacteria reach the stroma after adherence to corneal epithelial cells and how contact lenses increase the incidence of P. aeruginosa infection. Our broad theoretical model to explain the pathogenesis of contact lens related P. aeruginosa infection is that contact lens wear, because it provides a surface for biofilm formation, enables bacteria to persist at the ocular surface for long enough to adapt to defense factors that otherwise limit their ability to penetrate the corneal epithelium. Candidate corneal epithelial defense factors that might limit their ability to penetrate and which could also provide the driving force for bacterial adaptation include epithelial expressed antimicrobial peptides. Corneal epithelial cells are known to express a number of antimicrobial peptides capable of killing P. aeruginosa; including hBD 1-3 and cathelicidin LL-37, and it is known that exposure to antimicrobial peptides can induce differential gene expression in bacteria. In this dissertation, the hypothesis that was explored addressed only specific components of the broad theoretical model: That under normal circumstances, antimicrobial peptides expressed by the corneal epithelium limit P. aeruginosa translocation of the epithelium, but that prolonged exposure to corneal epithelia induces differential bacterial gene expression which enhances epithelial traversal.
Subjects/Keywords: Microbiology; Cellular biology; Antimicrobial Peptides; Corneal epithelium; Pseudomonas aeruginosa; Traversal
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APA (6th Edition):
Augustin, D. K. (2011). Identifying Mechanism of Traversal of Corneal Epithelial Cells by Pseudomonas aerugnosa. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/3zc143tt
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Augustin, Danielle Kristy. “Identifying Mechanism of Traversal of Corneal Epithelial Cells by Pseudomonas aerugnosa.” 2011. Thesis, University of California – Berkeley. Accessed January 18, 2021.
http://www.escholarship.org/uc/item/3zc143tt.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Augustin, Danielle Kristy. “Identifying Mechanism of Traversal of Corneal Epithelial Cells by Pseudomonas aerugnosa.” 2011. Web. 18 Jan 2021.
Vancouver:
Augustin DK. Identifying Mechanism of Traversal of Corneal Epithelial Cells by Pseudomonas aerugnosa. [Internet] [Thesis]. University of California – Berkeley; 2011. [cited 2021 Jan 18].
Available from: http://www.escholarship.org/uc/item/3zc143tt.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Augustin DK. Identifying Mechanism of Traversal of Corneal Epithelial Cells by Pseudomonas aerugnosa. [Thesis]. University of California – Berkeley; 2011. Available from: http://www.escholarship.org/uc/item/3zc143tt
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
11.
Stoycheva, Zarka / Стойчева, Зарка.
Ocular Diseases with Limbal Stem Cell Deficiency. Diagnostics, Microstructure Analysis and Therapeutic Approach // Очни заболявания с дефицит на лимбални стволови клетки – диагностика, микроструктурен анализ и терапевтичен подход.
Degree: 2019, Medical University of Varna
URL: http://repository.mu-varna.bg/handle/nls/504
► The eye is the viewing organ that helps to capture reflected light from the visible light spectrum. The first surface of the eye that is…
(more)
▼ The eye is the viewing organ that helps to capture reflected light from the visible light spectrum. The first surface of the eye that is in contact with the environment is the delicate epithelial layer that covers the anterior ocular surface. Preserving and maintaining the physiological features of the anterior ocular surface are of utmost importance for the comfort and vision of patients.
The main objective of this dissertation thesis is to perform a detailed micro-structural analysis of conditions related to limbal stem cell deficiency and to evaluate the clinical efficacy of applied therapeutic approaches.
Better knowledge of the limbal stem cells can help not only to understand the pathogenesis of conditions associated with their deficiency but may also improve the diagnosis, treatment, and hence the prognosis of patients.
Timely diagnosis allows a correct and well-judged therapeutic approach. With partial deficiency and diagnosis of early conditions associated with limbal stem cell deficiency (LSCD), conservative treatment may be sufficient. This results in better economic performance and improvement of subjective symptoms such as pain, irritation, photophobia, redness, and thus improves the quality of life. Amniotic membrane transplantation as a surgical method is currently a gold standard in the treatment of conditions associated with diseases of the anterior ocular surface, such as the lack of limbal stem cells. // [BG] Окото е зрителният орган, чрез който се улавят отразени от обектите светлинни лъчи от видимия спектър на светлината. Първата повърхност на окото, която е в досег с околната среда, е деликатният епителният слой, който покрива предна очна повърхност. Запазването и поддържането на физиологичните особености на предна очна повърхност са от изключително значение за комфорта и зрението на пациентите.
Основната цел на настоящия дисертационен труд е да се извърши подробен микроструктурен анализ на състояния свързани с недостатъчност на лимбални стволови клетки и да се направи оценка на клиничната ефективност от приложените терапевтични подходи.
По-доброто познаване на лимбалните стволови клетки може да помогне не само за разбирането на патогенезата на състоянията свързани с дефицитът им, но може и да подобри диагностиката, лечението и следователно прогнозата на пациентите. Навременната диагноза позволява правилен и своевременен терапевтичен подход. При частичен недостиг и диагностицирането на ранни състояния свързани с дефицит на лимбални стволови клетки консервативното лечение може да бъде достатъчно. Това води до по-добри икономически резултати, подобряване на субективната симптоматика като болка, дразнене, фотофобия, зачервяване и следователно качеството на живот. Трансплантацията на амниотична мембрана като хирургичен метод към момента остава златен стандарт в лечението на състояния свързани със заболявания на ПОП, каквото е и недостигът на лимбални стволови клетки.
Subjects/Keywords: limbal are; corneal epithelium; conjunctivalization; limbal stem cell deficiency; Офталмология / Ophthalmology
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APA (6th Edition):
Stoycheva, Zarka / Стойчева, . (2019). Ocular Diseases with Limbal Stem Cell Deficiency. Diagnostics, Microstructure Analysis and Therapeutic Approach // Очни заболявания с дефицит на лимбални стволови клетки – диагностика, микроструктурен анализ и терапевтичен подход. (Thesis). Medical University of Varna. Retrieved from http://repository.mu-varna.bg/handle/nls/504
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stoycheva, Zarka / Стойчева, Зарка. “Ocular Diseases with Limbal Stem Cell Deficiency. Diagnostics, Microstructure Analysis and Therapeutic Approach // Очни заболявания с дефицит на лимбални стволови клетки – диагностика, микроструктурен анализ и терапевтичен подход.” 2019. Thesis, Medical University of Varna. Accessed January 18, 2021.
http://repository.mu-varna.bg/handle/nls/504.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stoycheva, Zarka / Стойчева, Зарка. “Ocular Diseases with Limbal Stem Cell Deficiency. Diagnostics, Microstructure Analysis and Therapeutic Approach // Очни заболявания с дефицит на лимбални стволови клетки – диагностика, микроструктурен анализ и терапевтичен подход.” 2019. Web. 18 Jan 2021.
Vancouver:
Stoycheva, Zarka / Стойчева . Ocular Diseases with Limbal Stem Cell Deficiency. Diagnostics, Microstructure Analysis and Therapeutic Approach // Очни заболявания с дефицит на лимбални стволови клетки – диагностика, микроструктурен анализ и терапевтичен подход. [Internet] [Thesis]. Medical University of Varna; 2019. [cited 2021 Jan 18].
Available from: http://repository.mu-varna.bg/handle/nls/504.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stoycheva, Zarka / Стойчева . Ocular Diseases with Limbal Stem Cell Deficiency. Diagnostics, Microstructure Analysis and Therapeutic Approach // Очни заболявания с дефицит на лимбални стволови клетки – диагностика, микроструктурен анализ и терапевтичен подход. [Thesis]. Medical University of Varna; 2019. Available from: http://repository.mu-varna.bg/handle/nls/504
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
Comptour, Aurélie.
L'ATRA (acide tout trans rétinoïque), dérivé actif de la vitamine A, permet la cicatrisation de cellules épithélilales de cornée humaine HCE et de l'épithélium cornéen brulé par base chez le modèle souris. : ATRA (all trans retinoic acid), an active derivative of vitamin A, allows the healing of HCE human corneal epithelial cells and base-burned corneal epithelium in a mouse model.
Degree: Docteur es, Sciences de la vie et de la sante, 2015, Clermont-Ferrand 1
URL: http://www.theses.fr/2015CLF1MM07
► De par son rôle dans de nombreuses fonctions biologiques, la vitamine A est une molécule majeure et cruciale du développement embryonnaire à l’âge adulte. Celle-ci…
(more)
▼ De par son rôle dans de nombreuses fonctions biologiques, la vitamine A est une molécule majeure et cruciale du développement embryonnaire à l’âge adulte. Celle-ci est aussi, à l’heure actuelle, déjà largement utilisée comme agent thérapeutique pour de nombreuses pathologies affectant principalement la peau et les yeux (cancer, acné, psoriasis,brûlures oculaires) et certains cancers. Son action pro-cicatrisante - bien que largement étudiée grâce à de nombreux modèles humains et animaux - reste encore aujourd’hui mal caractérisée quant aux mécanismes d’action moléculaire (régulation de gènes) et cellulaire(migration, prolifération…) qu’elle utilise.Dans le but d’améliorer et de mieux maîtriser l’utilisation de la vitamine A dans le traitement de lésions telles que les brûlures oculaires, ce travail visait d’une part, à étudier, plus en détail,l’effet de l’acide tout-trans rétinoïque ou ATRA - dérivé le plus actif - sur la cicatrisation de ’épithélium cornéen en utilisant un modèle in vivo de brûlures cornéennes par base chez la souris. Son but était également de déterminer par quels processus cellulaires, l’ATRA agit, en utilisant cette fois-ci un modèle in vitro (cellules épithéliales cornéennes humaines). Enfin,nous nous sommes intéressés à la régulation de gènes cibles par l’ATRA au niveau de la sphère oculaire, connus pour être impliqués dans la dynamique de la MEC.Ainsi, nous avons démontré que l’ATRA permettait la cicatrisation de l’épithélium cornéen en agissant principalement sur la migration cellulaire. Puis nous avons identifié un gène :LOXL4 - membre de la famille des lysyl oxydases - dont l’expression est induite par l’ATRA,et nous avons prouvé que son rôle était essentiel dans la cicatrisation cornéenne, décrivant ainsi pour la première fois un lien génique direct et protéique entre la vitamine A, substance active et son action clinique pro-cicatrisante.
Because of its role in many biological functions, Vitamin A is a major and crucialmolecule from development to adulthood. Currently, it is largely used as therapeutic agent inseveral eye or skin pathologies (acne, psoriasis, ocular burns) and cancers. Its pro-healingproperties have been largely studied in human and animal models but molecular (generegulations) and cellular (migration, proliferation…) mechanisms of the vitamin A actionhave to be more detailed.In order to better improve and control its use in the treatment of lesions such as ocular burns,this work aimed to study, more in details, the effects of atRA (all-trans-retinoic acid), activederivative form of vitamin A, on corneal epithelium healing using an in vivo model of alkaliocular burns in mouse and then, to determine by which cellular process atRA acts, by usingthis time, an in vitro model (human corneal epithelial cells). Finally, we were interested intargeting genes regulation by atRA in the ocular sphere, specially known to be implied in theECM dynamic.First, we demonstrated that atRA improves corneal epithelium wound healing, essentially byacting on migration. Then, we identified a…
Advisors/Committee Members: Sapin, Vincent (thesis director).
Subjects/Keywords: ATRA; Vitamine A; Épithélium cornéen; Vitamin-A; ATRA; Corneal epithelium; 571.6
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APA (6th Edition):
Comptour, A. (2015). L'ATRA (acide tout trans rétinoïque), dérivé actif de la vitamine A, permet la cicatrisation de cellules épithélilales de cornée humaine HCE et de l'épithélium cornéen brulé par base chez le modèle souris. : ATRA (all trans retinoic acid), an active derivative of vitamin A, allows the healing of HCE human corneal epithelial cells and base-burned corneal epithelium in a mouse model. (Doctoral Dissertation). Clermont-Ferrand 1. Retrieved from http://www.theses.fr/2015CLF1MM07
Chicago Manual of Style (16th Edition):
Comptour, Aurélie. “L'ATRA (acide tout trans rétinoïque), dérivé actif de la vitamine A, permet la cicatrisation de cellules épithélilales de cornée humaine HCE et de l'épithélium cornéen brulé par base chez le modèle souris. : ATRA (all trans retinoic acid), an active derivative of vitamin A, allows the healing of HCE human corneal epithelial cells and base-burned corneal epithelium in a mouse model.” 2015. Doctoral Dissertation, Clermont-Ferrand 1. Accessed January 18, 2021.
http://www.theses.fr/2015CLF1MM07.
MLA Handbook (7th Edition):
Comptour, Aurélie. “L'ATRA (acide tout trans rétinoïque), dérivé actif de la vitamine A, permet la cicatrisation de cellules épithélilales de cornée humaine HCE et de l'épithélium cornéen brulé par base chez le modèle souris. : ATRA (all trans retinoic acid), an active derivative of vitamin A, allows the healing of HCE human corneal epithelial cells and base-burned corneal epithelium in a mouse model.” 2015. Web. 18 Jan 2021.
Vancouver:
Comptour A. L'ATRA (acide tout trans rétinoïque), dérivé actif de la vitamine A, permet la cicatrisation de cellules épithélilales de cornée humaine HCE et de l'épithélium cornéen brulé par base chez le modèle souris. : ATRA (all trans retinoic acid), an active derivative of vitamin A, allows the healing of HCE human corneal epithelial cells and base-burned corneal epithelium in a mouse model. [Internet] [Doctoral dissertation]. Clermont-Ferrand 1; 2015. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2015CLF1MM07.
Council of Science Editors:
Comptour A. L'ATRA (acide tout trans rétinoïque), dérivé actif de la vitamine A, permet la cicatrisation de cellules épithélilales de cornée humaine HCE et de l'épithélium cornéen brulé par base chez le modèle souris. : ATRA (all trans retinoic acid), an active derivative of vitamin A, allows the healing of HCE human corneal epithelial cells and base-burned corneal epithelium in a mouse model. [Doctoral Dissertation]. Clermont-Ferrand 1; 2015. Available from: http://www.theses.fr/2015CLF1MM07
Boston University
13.
Meyer, Jenna.
Eye-solating corneal innervation profiles to examine epithelial wound healing in a model of type II diabetes.
Degree: MS, Medical Sciences, 2016, Boston University
URL: http://hdl.handle.net/2144/19491
► INTRODUCTION: The cornea forms the anterior-most barrier of the eye, consisting of a non-keratinized pseudostratified squamous epithelium, a collagen-based stroma, and an endothelium. It is…
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▼ INTRODUCTION: The cornea forms the anterior-most barrier of the eye, consisting of a non-keratinized pseudostratified squamous epithelium, a collagen-based stroma, and an endothelium. It is completely avascular, yet the most densely innervated structure in the human body. The sensory nerves project from the ophthalmic branch of the trigeminal cranial nerve into the limbal/stromal interface. From there, the nerves branch and ascend into Bowman’s membrane, a basal lamina delineating the epithelium from the stroma, and project into the epithelium as free nerve endings. Injury to the corneal epithelium can potentially lead to impaired vision if the wound healing process is not properly initiated. Immediately after injury, nucleotides such as ATP are released and bind to purinergic receptors known to be located in epithelial cell membranes, thereby initiating epithelial cell migration to close the wound. Malfunctions in the interactions between the corneal nerves and their epithelial counterparts during the wound healing process are thought to contribute to the attenuated wound healing characteristic of diabetes. However, the precise nature of these interactions, how they facilitate wound healing, and how they are impaired in diabetes, is not well understood.
OBJECTIVES: Previously, our lab has shown that a member of purinergic family receptors (P2X7) is localized in the basal epithelial cells and becomes relocated to the leading edge of the wound after injury. When the relocation is inhibited, migration is attenuated. Additionally, it is known that diabetic mouse models display slower wound healing rates. The present study has three aims: (1) to replicate the characteristic sub-basal whorl organization of the corneal nerves in organ-cultured corneas; (2) to elucidate the connections between patterns of corneal innervation and purinergic receptor expression; and (3) to understand how these patterns interact to facilitate normal wound healing and how these interactions are disrupted in a diabetic model.
METHODS: Our approach was to use immunohistochemistry of dissected mouse and to visualize the tissue using confocal microscopy. Sensory innervation profiles from diet induced obesity (DIO) mouse corneas and their wildtype C57Bl6 counterparts were compared in unwounded and wounded tissue. To image the nerves a methanol fixation protocol was optimized to examine the sub-basal plexus and the apical nerves. Corneas were dissected, stained with beta III-tubulin, which identifies nerves, and with an antibody to the P2X7 purinergic receptor, which is expressed in the epithelium and nerves. Trephine induced epithelial abrasion injuries were made on separate DIO and control models to compare re-epithelialization and re-innervation between the diseased and healthy states. Corneas were imaged using a Zeiss LSM 700 laser scanning confocal microscope and optical images were taken through the cornea over a distance averaging 115 microns. Corneas were imaged using a macro tiling plugin, stitching 3x3 optical z-stacks into composite…
Subjects/Keywords: Biochemistry; P2X7; Cornea; Corneal nerves; Epithelium; Purinergic receptor; Wound healing
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APA (6th Edition):
Meyer, J. (2016). Eye-solating corneal innervation profiles to examine epithelial wound healing in a model of type II diabetes. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/19491
Chicago Manual of Style (16th Edition):
Meyer, Jenna. “Eye-solating corneal innervation profiles to examine epithelial wound healing in a model of type II diabetes.” 2016. Masters Thesis, Boston University. Accessed January 18, 2021.
http://hdl.handle.net/2144/19491.
MLA Handbook (7th Edition):
Meyer, Jenna. “Eye-solating corneal innervation profiles to examine epithelial wound healing in a model of type II diabetes.” 2016. Web. 18 Jan 2021.
Vancouver:
Meyer J. Eye-solating corneal innervation profiles to examine epithelial wound healing in a model of type II diabetes. [Internet] [Masters thesis]. Boston University; 2016. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/2144/19491.
Council of Science Editors:
Meyer J. Eye-solating corneal innervation profiles to examine epithelial wound healing in a model of type II diabetes. [Masters Thesis]. Boston University; 2016. Available from: http://hdl.handle.net/2144/19491
University of Edinburgh
14.
Mort, Richard Lester.
Stem cell function in the mouse corneal epithelium.
Degree: PhD, 2007, University of Edinburgh
URL: http://hdl.handle.net/1842/8187
► Limbal stem cells maintain the corneal epithelium through a process of clonal growth and ordered migration. In X-inactivation mosaic female mice, that express LacZ from…
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▼ Limbal stem cells maintain the corneal epithelium through a process of clonal growth and ordered migration. In X-inactivation mosaic female mice, that express LacZ from one of their X-chromosomes, random clumps of LacZ-positive cells are seen in the cornea at 3-6 weeks of life. This pattern resolves between 6-10 weeks forming radial stripes thought to represent chords of clonally related, inwardly migrating cells. By measuring the number and width of stripes and correcting for the effects of different proportions of LacZ-positive cells, an estimate of the number of coherent stem cell clones maintaining the tissue can be derived. Analysis at 5 ages demonstrated that the estimated number of coherent stem cell clones is reduced from ~100 at 15 weeks to ~50 at 39 weeks and is then stable at least until 52 weeks. An automated method was developed using image analysis software to analyse these striping patterns. This method produced results that did not differ significantly from the above. The dosage of the transcription factor Pax6 is crucial for normal eye development. In Pax6 heterozygous animals the estimated number of coherent stem cell clones is reduced to ~50 at 15 weeks with no further reduction up to 30 weeks. Mice hemizygous for the PAX77 transgene over-express human PAX6. In PAX77 hemizygous X-inactivation mosaics, estimated clone number was similarly reduced to ~50 with no further decline. Mice heterozygous for both Gli3 and Pax6 have a distinct striping phenotype, highlighted by an increase in coherent clones. When the corneal epithelium is injured the surrounding epithelial cells migrate along the corneal stroma to cover the wound. X-gal staining of healed, centrally wounded X-inactivation eyes reveals that striping patterns are reconstituted during wound healing in ex-vivo culture. In GFP mosaics the healing process can be imaged using time-lapse confocal microscopy. This technique demonstrated that clones remain contiguous throughout their migration. Healing of peripheral wounds was observed to form de-novo whorling patterns, revealing that basal cells in the epithelium can migrate both away from and towards the limbal region.
Subjects/Keywords: corneal epithelium; limbal stem cells
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APA (6th Edition):
Mort, R. L. (2007). Stem cell function in the mouse corneal epithelium. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/8187
Chicago Manual of Style (16th Edition):
Mort, Richard Lester. “Stem cell function in the mouse corneal epithelium.” 2007. Doctoral Dissertation, University of Edinburgh. Accessed January 18, 2021.
http://hdl.handle.net/1842/8187.
MLA Handbook (7th Edition):
Mort, Richard Lester. “Stem cell function in the mouse corneal epithelium.” 2007. Web. 18 Jan 2021.
Vancouver:
Mort RL. Stem cell function in the mouse corneal epithelium. [Internet] [Doctoral dissertation]. University of Edinburgh; 2007. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1842/8187.
Council of Science Editors:
Mort RL. Stem cell function in the mouse corneal epithelium. [Doctoral Dissertation]. University of Edinburgh; 2007. Available from: http://hdl.handle.net/1842/8187
Stellenbosch University
15.
Tchokonte-Nana, Venant.
Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas.
Degree: PhD, Biomedical Sciences, 2011, Stellenbosch University
URL: http://hdl.handle.net/10019.1/6828
► ENGLISH ABSTRACT: Diabetes mellitus is amongst the leading causes of morbidity and mortality in the world, affecting young, adult and old people. Beta cell replacement…
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▼ ENGLISH ABSTRACT: Diabetes mellitus is amongst the leading causes of morbidity and mortality in the world, affecting
young, adult and old people. Beta cell replacement therapy for insulin delivery remains the ultimate
remedy for diabetes. However, insufficient donor pancreas and the use of immunosuppressive drugs
prevent the wide-spread of this therapy. Other avenues of self generated beta cells within the organ
itself need to be explored. Therefore, understanding the chronobiology of cellular mechanisms in
the lineage of beta cell induced neogenesis is a valuable tool in improving beta cell replacement in
patients with diabetes. The aim of this study was to induce recapitulation of the morpho-genetic
sequence of endocrine cells development in the pancreas of rats after the pancreatic duct ligation
(PDL) procedure. Serial sections of PDL tissues of the pancreas were obtained from 78 Sprague-
Dawley rats and were assessed morphologically. The immunofluorescent tissues were statistically
analysed using a computerized morphometry technique. The protein expression indices of
Caspase3, Insulin, Pdx1, Ngn3, NeuroD and Pax6 were quantified. The efficiency levels of coexpression
of these homeodomain proteins separately with insulin were defined by the ratio of the
mean value of insulin expression to the mean value of their respective protein expression. The
morphological changes were characterized by the appearance of granulated acinar cells at 6 hours
post-PDL and the proliferation of endocrine tissues from 84 hours through to 120 hours. The
morpho-immunofluorescent evaluation showed the highest immunoreactivity of Caspase3 and Pdx1
at 6 hours, Ngn3 at 36 hours, Pax6 and insulin at 84 hours while NeuroD expression was at 120
hours. The immunohistofluorescent analysis showed that caspase3 and Pdx1 were the first to be
expressed at 6 hours while the insulin and NeuroD expression appeared later at 84 hours and 120
hours, respectively. However, Pax6 expression was continuous across time periods post-PDL, while
Ngn3 expression showed a peak at 36 hours. The efficiency (highest and earliest expression) of co-expression of all these homeodomain proteins with insulin was restricted between 12 hours and 24
hours. The optimal efficiency was at 12 hours by Ngn3 with insulin. A good efficiency was shown
for Pdx1 with insulin, NeuroD with insulin and Pax6 with insulin at 12 hours and 24 hours,
respectively. A low efficiency was observed for insulin and caspase3 co-expression at 24 hours.
This study suggests that for transplantation, PDL tissues harvested at an early time post-PDL
(between 12 and 24 hours) could yield a higher success rate; the study also provides evidence for a
connection between morphological changes in the PDL pancreas and the protein synthesis
necessary for the lineage of endocrine cell development.
AFRIKAANSE OPSOMMING: Diabetes Mellitus resorteer onder die vernaamste oorsake van morbiditeit en mortaliteit wêreldwyd,
en tuister jongmense, volwassenes en bejaardes. Daar bestaan egter…
Advisors/Committee Members: Page, Benedict J., Du Toit, Don F., University of Stellenbosch. Faculty of Health Sciences. Dept. of Biomedical Sciences. Anatomy and Histology..
Subjects/Keywords: Histology; Dissertations – Anatomy; Dissertations – Histology; Endocrine cell development; Biomedical Sciences. Anatomy and Histology
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APA ·
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Export
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APA (6th Edition):
Tchokonte-Nana, V. (2011). Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/6828
Chicago Manual of Style (16th Edition):
Tchokonte-Nana, Venant. “Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas.” 2011. Doctoral Dissertation, Stellenbosch University. Accessed January 18, 2021.
http://hdl.handle.net/10019.1/6828.
MLA Handbook (7th Edition):
Tchokonte-Nana, Venant. “Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas.” 2011. Web. 18 Jan 2021.
Vancouver:
Tchokonte-Nana V. Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas. [Internet] [Doctoral dissertation]. Stellenbosch University; 2011. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10019.1/6828.
Council of Science Editors:
Tchokonte-Nana V. Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas. [Doctoral Dissertation]. Stellenbosch University; 2011. Available from: http://hdl.handle.net/10019.1/6828
Tampere University
16.
Puistola, Paula.
Novel bioink design for 3D bioprinting of human pluripotent stem cell derived corneal epithelial cells
.
Degree: 2020, Tampere University
URL: https://trepo.tuni.fi/handle/10024/123138
► Objectives: The correct function and structure of cornea is essential for vision. Cornea is maintained by limbal epithelial stem cells (LESCs), and the lack of…
(more)
▼ Objectives: The correct function and structure of cornea is essential for vision. Cornea is maintained by limbal epithelial stem cells (LESCs), and the lack of functional LESCs in a disease called limbal stem cell deficiency (LSCD) can lead to blindness. The traditional treatment for corneal blindness is a corneal transplant. However, there is a severe shortage of cornea donors and the transplants lack functional LESCs. Thus, a corneal transplant cannot be used as a treatment for LSCD. The field of tissue engineering aims to restore, replace and regenerate damaged native tissues, such as develop artificial corneas. Yet, the conventional methods fail to mimic the native-like cellular variety and specific microstructure. Moreover, they lack the ability for precise positioning of cells and materials into a three-dimensional (3D) environment. 3D bioprinting offers a possibility to overcome these issues due to its better control, accuracy and customizability. The main challenge in 3D bioprinting is the lack of bioprintable, cell-laden bioinks with suitable properties to guide the desired cell behaviour. The aim in this thesis was to design and optimize a novel bioink and bioprinting conditions in order to 3D bioprint human pluripotent stem cell (hPSC) -derived corneal epithelium mimicking tissue.
Materials and methods: A novel bioink composition was done combining human and recombinant sourced extracellular matrix proteins. The native human cornea was used as a source of inspiration in the development. Moreover, two crosslinking strategies were combined. First, the printability of the bioink and the printing parameters for extrusion-based bioprinting were tested and determined. The hPSC-derived LESCs (hPSC-LESCs) were produced, and the bioprinting conditions, including the ultra violet (UV) light exposure and printing substrate, were optimized. The response to different bioprinting conditions and behaviour of the bioprinted hPSC-LESCs were analysed with phase contrast microscopy, proliferation and live/dead assays, and immunofluorescence analysis. Finally, the bioink was characterized by analysing its swelling behaviour, transparency and rheological properties.
Results and conclusions: Overall, the developed bioink was well extrudable and had good transparency. The bioink supported the proliferation and maturation of the hPSC-LESCs. UV exposure did not decrease the cell viability (> 88%), however, it was observed to affect the crosslinking density and the stiffness of the material considerably. The bioprinted hPSC-LESCs preferred softer, highly viscous material, and bioprinting without UV exposure resulted in the most stratified epithelium. From the printing substrates, MatrigelTM coating provided the best results in regards to the cell proliferation and adhesion. However, due to the softness of MatrigelTM, the bioprinted epithelium showed shrinkage leading to partially ruptured epithelium. Therefore, further optimization of the printing substrate is required. Furthermore, due to the low crosslinking degree of the…
Subjects/Keywords: cornea
;
corneal epithelium
;
corneal tissue engineering
;
3D bioprinting
;
bioink
;
human pluripotent stem cell
;
limbal epithelial stem cell
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APA ·
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APA (6th Edition):
Puistola, P. (2020). Novel bioink design for 3D bioprinting of human pluripotent stem cell derived corneal epithelial cells
. (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/123138
Chicago Manual of Style (16th Edition):
Puistola, Paula. “Novel bioink design for 3D bioprinting of human pluripotent stem cell derived corneal epithelial cells
.” 2020. Masters Thesis, Tampere University. Accessed January 18, 2021.
https://trepo.tuni.fi/handle/10024/123138.
MLA Handbook (7th Edition):
Puistola, Paula. “Novel bioink design for 3D bioprinting of human pluripotent stem cell derived corneal epithelial cells
.” 2020. Web. 18 Jan 2021.
Vancouver:
Puistola P. Novel bioink design for 3D bioprinting of human pluripotent stem cell derived corneal epithelial cells
. [Internet] [Masters thesis]. Tampere University; 2020. [cited 2021 Jan 18].
Available from: https://trepo.tuni.fi/handle/10024/123138.
Council of Science Editors:
Puistola P. Novel bioink design for 3D bioprinting of human pluripotent stem cell derived corneal epithelial cells
. [Masters Thesis]. Tampere University; 2020. Available from: https://trepo.tuni.fi/handle/10024/123138
17.
Marcon, Alexandre Seminoti.
Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP).
Degree: PhD, Oftalmologia, 2006, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-16102014-085907/
;
► Objetivo: Analisar a influência da espessura corneana central na acuidade visual (AV) corrigida após transplante de córnea endotelial lamelar profundo (TCELP). Métodos: Foram estudados de…
(more)
▼ Objetivo: Analisar a influência da espessura corneana central na acuidade visual (AV) corrigida após transplante de córnea endotelial lamelar profundo (TCELP). Métodos: Foram estudados de forma prospectiva 155 olhos de 127 pacientes portadores de ceratopatia bolhosa ou distrofia endotelial de Fuchs no sexto mês de pós-operatório do TCELP, entre março de 2000 e março de 2005. Foram excluídos pacientes com outras alterações oculares que justificassem baixa AV. Todos os pacientes foram submetidos à avaliação oftálmica, quando foram determinadas AV corrigida, por meio de exame refratométrico, e espessura corneana central, através da paquimetria ultra-sônica. As técnicas usada foram previamente descritas. Os olhos foram agrupados de acordo com as medidas de AV: grupo I (20/20 - 20/30), grupo II (20/40 - 20/50), grupo III (20/60 - 20/80), grupo IV (20/100 - 20/400). Para correlação com paquimetria e análise estatística, as medidas de AV foram convertidas da tabela de Snellen para a tabela logarítmica (logMAR). Foram criadas variáveis categóricas para expressar status de faixa de normalidade de espessura corneana (entre 495 e 651 ?m), usando como pontos de corte valores encontrados na literatura. Resultados: A média, o desvio padrão e a variação da paquimetria foi: grupo I (n=38) 571 ±80 um, 408 a 784 um; grupo II (n=79) 598 ±80 um, 437 a 816 um; grupo III (n=30) 605 ±99 um, 454 a 945 um e grupo IV (n=8) 607 ±120 ?m, 410 a 781 ?m. Analisando o resultado da AV e a porcentagem de casos com espessura corneana acima de 651 um, foi observada associação linear significativa (P=0,037; ?2 de tendência linear) entre o aumento da paquimetria e a piora da AV. Analisando a associação entre os grupos de AV e a porcentagem de casos com espessura corneana abaixo da faixa de normalidade (<495 um), não foi encontrada significância estatística (P=0,92; x2 de Pearson). Quando analisado o resultado visual do grupo I em relação ao resultado dos grupos II+III+IV em conjunto, observou-se que somente 13% dos casos do grupo I e 30% dos casos dos demais grupos apresentaram espessura corneana maior do que 651 ?m. Essa correlação demonstrou significância estatística limítrofe (P=0,066; x2 de Pearson com correção de Yates). Conclusão: Observou-se associação linear significativa entre piora da AV corrigida e aumento da espessura corneana central. Quando analisados somente casos com paquimetria abaixo da faixa de normalidade, não foi observada associação significativa entre piora da AV corrigida e espessura corneana central
Purpose: To analyze the influence of central corneal thickness in the corrected visual acuity (VA) after deep lamellar endothelial corneal keratoplasty (DLEK). Methods: Retrospective study of 155 eyes of 127 patients 6 months post-op DLEK between March 2000 and March 2005. These patients had been previously diagnosed with either bullous keratopathy or Fuch\'s endothelial dystrophy. Patients with other ophthalmic conditions that could cause loss of vision were excluded. All patients underwent ophthalmic evaluation to determine…
Advisors/Committee Members: Jose, Newton Kara.
Subjects/Keywords: Acuidade visual; Comparative study; Córnea/anatomia & histologia; Corneal transplantation/methods; Corneal/anatomy & histology; Endotélio da córnea; Endothelium/corneal; Estudo comparativo; Measures; Medidas; Transplante de córnea/métodos; Visual acuity
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Marcon, A. S. (2006). Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5149/tde-16102014-085907/ ;
Chicago Manual of Style (16th Edition):
Marcon, Alexandre Seminoti. “Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP).” 2006. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5149/tde-16102014-085907/ ;.
MLA Handbook (7th Edition):
Marcon, Alexandre Seminoti. “Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP).” 2006. Web. 18 Jan 2021.
Vancouver:
Marcon AS. Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP). [Internet] [Doctoral dissertation]. University of São Paulo; 2006. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-16102014-085907/ ;.
Council of Science Editors:
Marcon AS. Influência da espessura corneana na acuidade visual corrigida após transplante de córnea endotelial lamelar profundo (TCELP). [Doctoral Dissertation]. University of São Paulo; 2006. Available from: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-16102014-085907/ ;
18.
Hammadi, Shumoos T. H.
Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images.
Degree: PhD, 2018, University of Bradford
URL: http://hdl.handle.net/10454/16924
► Diabetic Peripheral Neuropathy (DPN) is one of the most common types of diabetes that can affect the cornea. An accurate analysis of the corneal epithelium…
(more)
▼ Diabetic Peripheral Neuropathy (DPN) is one of the most common types of diabetes that can affect the cornea. An accurate analysis of the corneal epithelium nerve structures and the corneal endothelial cell can assist early diagnosis of this disease and other corneal diseases, which can lead to visual impairment and then to blindness. In this thesis, fully-automated segmentation and quantification algorithms for processing and analysing sub-basal epithelium nerves and endothelial cells are proposed for early diagnosis of diabetic neuropathy in Corneal Confocal Microscopy (CCM) images. Firstly, a fully automatic nerve segmentation system for corneal confocal microscope images is proposed. The performance of the proposed system is evaluated against manually traced images with an execution time of the prototype is 13 seconds. Secondly, an automatic corneal nerve registration system is proposed. The main aim of this system is to produce a new informative corneal image that contains structural and functional information. Thirdly, an automated real-time system, termed the Corneal Endothelium Analysis System (CEAS) is developed and applied for the segmentation of endothelial cells in images of human cornea obtained by In Vivo CCM. The performance of the proposed CEAS system was tested against manually traced images with an execution time of only 6 seconds per image. Finally, the results obtained from all the proposed approaches have been evaluated and validated by an expert advisory board from two institutes, they are the Division of Medicine, Weill Cornell Medicine-Qatar, Doha, Qatar and the Manchester Royal Eye Hospital, Centre for Endocrinology and Diabetes, UK.
Subjects/Keywords: Medical imaging; Diabetic peripheral neuropathy; Corneal confocal microscopy; Automatic nerve segmentation; Corneal sub-basal epithelium; Anisotropic diffusion filtering; Corneal endothelial cells; Automatic cell segmentation; Fast Fourier transform; Watershed transformation
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APA (6th Edition):
Hammadi, S. T. H. (2018). Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images. (Doctoral Dissertation). University of Bradford. Retrieved from http://hdl.handle.net/10454/16924
Chicago Manual of Style (16th Edition):
Hammadi, Shumoos T H. “Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images.” 2018. Doctoral Dissertation, University of Bradford. Accessed January 18, 2021.
http://hdl.handle.net/10454/16924.
MLA Handbook (7th Edition):
Hammadi, Shumoos T H. “Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images.” 2018. Web. 18 Jan 2021.
Vancouver:
Hammadi STH. Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images. [Internet] [Doctoral dissertation]. University of Bradford; 2018. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10454/16924.
Council of Science Editors:
Hammadi STH. Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images. [Doctoral Dissertation]. University of Bradford; 2018. Available from: http://hdl.handle.net/10454/16924
19.
Sukekava, Flávia.
Estudo comparativo da remodelação das estruturas do rebordo alveolar após elevação do retalho.
Degree: PhD, Periodontia, 2013, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/23/23146/tde-04072013-154653/
;
► O objetivo deste estudo foi descrever e comparar algumas características das estruturas alveolares após cirurgia de elevação de retalho. Cinco cães beagle tiveram seus segundos…
(more)
▼ O objetivo deste estudo foi descrever e comparar algumas características das estruturas alveolares após cirurgia de elevação de retalho. Cinco cães beagle tiveram seus segundos e terceiros pré-molares superiores raspados e alisados com instrumentos manuais e ultrassônicos. Do lado direito da maxila (Grupo teste) um retalho total foi elevado sobre o segundo e o terceiro pré-molares, de maneira a expor o osso alveolar até a junção muco-gengival, durante 15 minutos. Após esse período, os retalhos foram irrigados com soro fisiológico e retornados às suas posições iniciais e mantidos em posição com fio de sutura reabsorvível. Na região contralateral (Grupo controle), dentes/gengiva permaneceram intactos. Após três meses de cicatrização, os animais foram ortotanasiados e biópsias contendo tecidos mole e duro ao redor dos dentes foram obtidas das áreas teste e controle. Os blocos de biópsias foram preparados para analise morfométrica. Sobre os cortes, foram obtidas medidas lineares de ambos os grupos nos tecidos mole e duro, sendo que essas medidas foram obtidas em um, três e cinco milímetros abaixo da junção cemento-esmalte para: espessura do osso alveolar propriamente dito e do osso alveolar, espessura do ligamento periodontal e somente sobre tecido mole: espessura e altura gengival, comprimento do epitélio juncional. Valores médios e desvio padrão foram calculados usando o animal como unidade estatística. Teste t de Student pareado foi utilizado sendo p<0.05 considerado como diferença estatisticamente significante. A análise morfométrica mostrou que noventa dias após elevação do retalho não havia mais sinais de modelação óssea e as dimensões dos tecidos moles e duros periodontais sofreram alterações, com diferença estatisticamente significante: espessura da gengiva supra-crestal e do osso alveolar propriamente dito e comprimento do epitélio juncional (p<0.05). Os resultados sugerem que após elevação do retalho, espessura da gengiva supra-crestal, bem como o comprimento do epitélio juncional e altura da crista óssea sofreram alterações em suas dimensões significativamente; osso alveolar propriamente dito tem espessura constante ao longo do osso alveolar e também representa grande parte do osso alveolar em sua porção mais próxima à junção cemento-esmalte.
The aim of this study was to describe and to compare some characteristics of alveolar ridge structures after flap surgery. Five beagle dogs had their upper second and third premolars selected in both quadrants to be scaled and planned. On the right side (Test group) the buccal flap was elevated on the second and third premolar, exposing the alveolar bone, over the muco-gingival junction for 15 minutes. After that, the flaps were irrigated with saline and returned to the original position and sutured with bioabsorbable mattress. On the contra lateral side (Control group) the teeth and gingiva were maintained intact. After three months of healing, the dogs were sacrificed to provide biopsies containing soft and hard tissue around teeth. The biopsies were prepared for…
Advisors/Committee Members: Lima, Luiz Antonio Pugliesi Alves de.
Subjects/Keywords: Anatomia; Anatomy; Histologia; Histology; Periodontia; Periodontics
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APA ·
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APA (6th Edition):
Sukekava, F. (2013). Estudo comparativo da remodelação das estruturas do rebordo alveolar após elevação do retalho. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/23/23146/tde-04072013-154653/ ;
Chicago Manual of Style (16th Edition):
Sukekava, Flávia. “Estudo comparativo da remodelação das estruturas do rebordo alveolar após elevação do retalho.” 2013. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/23/23146/tde-04072013-154653/ ;.
MLA Handbook (7th Edition):
Sukekava, Flávia. “Estudo comparativo da remodelação das estruturas do rebordo alveolar após elevação do retalho.” 2013. Web. 18 Jan 2021.
Vancouver:
Sukekava F. Estudo comparativo da remodelação das estruturas do rebordo alveolar após elevação do retalho. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/23/23146/tde-04072013-154653/ ;.
Council of Science Editors:
Sukekava F. Estudo comparativo da remodelação das estruturas do rebordo alveolar após elevação do retalho. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/23/23146/tde-04072013-154653/ ;
20.
Waghaye, Jitendra Yogesh.
Histomorphological, histochemical and immunohistological
studies on some lymphoid organs in goat (Capra hircus).
Degree: 2011, Maharashtra Animal and Fishery Sciences University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/2144
Bibliography p. i-x, Appendices p.
xi-xix
Advisors/Committee Members: Bhamburkar, V R.Subjects/Keywords: Anatomy; Histology; Embryology
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Waghaye, J. Y. (2011). Histomorphological, histochemical and immunohistological
studies on some lymphoid organs in goat (Capra hircus). (Thesis). Maharashtra Animal and Fishery Sciences University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/2144
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Waghaye, Jitendra Yogesh. “Histomorphological, histochemical and immunohistological
studies on some lymphoid organs in goat (Capra hircus).” 2011. Thesis, Maharashtra Animal and Fishery Sciences University. Accessed January 18, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/2144.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Waghaye, Jitendra Yogesh. “Histomorphological, histochemical and immunohistological
studies on some lymphoid organs in goat (Capra hircus).” 2011. Web. 18 Jan 2021.
Vancouver:
Waghaye JY. Histomorphological, histochemical and immunohistological
studies on some lymphoid organs in goat (Capra hircus). [Internet] [Thesis]. Maharashtra Animal and Fishery Sciences University; 2011. [cited 2021 Jan 18].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/2144.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Waghaye JY. Histomorphological, histochemical and immunohistological
studies on some lymphoid organs in goat (Capra hircus). [Thesis]. Maharashtra Animal and Fishery Sciences University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/2144
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Stellenbosch University
21.
Page, Benedict J. (Benedict John).
A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model.
Degree: PhD, Biomedical Sciences, 2000, Stellenbosch University
URL: http://hdl.handle.net/10019.1/51573
► ENGLISH ABSTRACT: Diabetes Mellitus (DM) is synonymous with "B-cell failure". Ligation of the pancreatic duct distally to its confluence into the bile duct has been…
(more)
▼ ENGLISH ABSTRACT: Diabetes Mellitus (DM) is synonymous with "B-cell failure". Ligation of the pancreatic
duct distally to its confluence into the bile duct has been shown to induce endocrine
tissue regeneration from a number of probable sources. The cells responsible for
regeneration are supposed to possess either dormant pluripotent stem cell ability and/or
the plasticity to undergo metaplasia to form new and surplus endocrine tissue able to
replace pathologically and/or experimentally compromised pancreas. The sequence of
events (cell lineage) during this process of neogenesis, has been the source of
controversy for quite some time as various studies suggest that the cell lineage differs
from in vivo and in vitro studies, according to experimental model and species of
laboratory animal.
The object of this study was to utilise an established experimental laboratory animal
model to study islet morphological changes, neogenesis and or both in vivo. Further
aims of the study were to determine the extent, sequence and magnitude of pancreatic
duct ligation (PDL) induced endocrine neogenesis/morphogenesis in a laboratory rat
model using occlusive pancreatic duct ligation.
PDL's were performed on six groups of 25 normal adult Sprague-Dawley (SD) rats
(300g+) according to the method of Hultquist and Jonsson (1965). Experimental
animals were sacrificed at 12 hr intervals from day one post-PDL to day 10 and every
24 hrs thereafter to day 14 as described by Wang, Klëppel, Bouwens (1995). Animals
received BrdU (a thymidine marker and cell proliferation indicator) 50mglkg
intraperitoneally as described by Wang et al. (1995), one hour prior to removal of the
pancreas after which it was fixed in Bouin's solution and histologically processed.
Seven consecutive 3-6 urn thick serial sections were sequentially stained with H & E,
insulin (I), glucagon (G), somatostatin (ST), pancreatic polypeptide (PP), neuropeptide
tyrosine (NPY) and peptide tyrosine tyrosine (PYY). Immunolabeling was done
according to the method of Guesdon, Temynck , Avrameas (1979). Double
immunolabeling for BrdU and each pancreatic peptide was performed on the sections
on days 3,5, 7, 9 and 11 as described by Wang et al (1994). Cellular transformation between one and 3Yz days was characterised by simultaneous
total deletion and/or transdifferentiation of the acinar compartment and the appearance
of immunoreactive cells for I (11.53 ±1.5%), G (1.85 ±0.8%), pp (1.50 ±0.09%), and
ST (1.96 ±0.24%). Changes in the endocrine composition in existing islets, occurred
along a pathway that saw PP- and ST-cells invading the islet core, islet mantle glucagon
deletion and random appearance of all endocrine cell types within the inter-islet
interstitium on day 3Yz. Days 4 to 6Yz saw further endocrine expansion while days 7 to
14 were distinguished by islet reconstitution and consolidation. NPY immunoreactivity
appeared on day 4Y2 and persisted intermittently throughout while PVV first appeared
on day 4 and disappeared after day 7Yz.
The…
Advisors/Committee Members: Du Toit, Don F., Wolfe-coote, Sonia A., Stellenbosch University. Faculty of Medicine & Health Science. Dept. of Biomedical Sciences..
Subjects/Keywords: Anatomy and histology
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Page, B. J. (. J. (2000). A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/51573
Chicago Manual of Style (16th Edition):
Page, Benedict J (Benedict John). “A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model.” 2000. Doctoral Dissertation, Stellenbosch University. Accessed January 18, 2021.
http://hdl.handle.net/10019.1/51573.
MLA Handbook (7th Edition):
Page, Benedict J (Benedict John). “A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model.” 2000. Web. 18 Jan 2021.
Vancouver:
Page BJ(J. A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model. [Internet] [Doctoral dissertation]. Stellenbosch University; 2000. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10019.1/51573.
Council of Science Editors:
Page BJ(J. A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model. [Doctoral Dissertation]. Stellenbosch University; 2000. Available from: http://hdl.handle.net/10019.1/51573
Stellenbosch University
22.
Mazyala, Eric John.
A morphological study of the dermal fibroblast.
Degree: MScMedSc (Biomedical Sciences. Anatomy and Histology, Biomedical Sciences, 2008, Stellenbosch University
URL: http://hdl.handle.net/10019.1/4079
Subjects/Keywords: Anatomy and histology
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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Manager
APA (6th Edition):
Mazyala, E. J. (2008). A morphological study of the dermal fibroblast. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/4079
Chicago Manual of Style (16th Edition):
Mazyala, Eric John. “A morphological study of the dermal fibroblast.” 2008. Masters Thesis, Stellenbosch University. Accessed January 18, 2021.
http://hdl.handle.net/10019.1/4079.
MLA Handbook (7th Edition):
Mazyala, Eric John. “A morphological study of the dermal fibroblast.” 2008. Web. 18 Jan 2021.
Vancouver:
Mazyala EJ. A morphological study of the dermal fibroblast. [Internet] [Masters thesis]. Stellenbosch University; 2008. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10019.1/4079.
Council of Science Editors:
Mazyala EJ. A morphological study of the dermal fibroblast. [Masters Thesis]. Stellenbosch University; 2008. Available from: http://hdl.handle.net/10019.1/4079
University of Cambridge
23.
VanBuren, Collin.
Assessing sources of variation in amphibian skin thickness: ecological and evolutionary implications.
Degree: PhD, 2017, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/267899
► The skin is the largest organ of the body and provides many functions. Among tetrapod vertebrates, amphibian skin is semi-permeable and responsible for a greater…
(more)
▼ The skin is the largest organ of the body and provides many functions. Among tetrapod vertebrates, amphibian skin is semi-permeable and responsible for a greater proportion of water absorption and gas exchange. Myriad factors affect the physiological performance of amphibian skin. Morphological traits linked with amphibian skin physiology or ecology have remained difficult to discern because of a lack of quantitative comparative research and the discovery of sources of intraspecific variation that are mostly ignored in study designs. This thesis aims to address the effects of these sources of variation using a trait that is known to vary between sexes, among seasons, and among body regions and thought to be linked with physiology or ecology, skin thickness. The first source of variation addressed is sexual dimorphism. Specimens of the white-lipped treefrog, Litoria infrafrenata, that display sexual dimorphism in body size and skin thickness were used to test if body size was the main determinate of sexually dimorphic skin thickness. Size corrected values did not significantly differ between males and females, although the sample size was small. Seasonal variation in skin thickness has also been documented in some species, so the American bullfrog (Lithobates catesbeianus), the Northern leopard frog (L. pipiens), and the spring peeper (Pseudacris crucifer) from multiple months of the year were sampled to determine if skin thickness increased in the autumn or winter months. Seasonal skin thickening was only detected in L. catesbeianus, and skin from autumn and winter was significantly thicker than from earlier in the year. This pattern was also detectable in museum specimens collected 80 years ago, although the signal was damped, possibly due to preservation. Using a dataset of 10 species and published data, a general pattern was uncovered whereby the dorsal skin is the thickest region and the ventral thigh region is the thinnest. However, this pattern is not always true for every individual of every species (L. pipiens and P. crucifer) and in some species the dorsal skin is thinnest (Bokermannohyla alvarengai and Litoria infrafrenata). The same dataset found that skin thickness is significantly related to body size, as was found in the chapter on Litoria infrafrenata. Summer specimens of Lithobates catesbeianus were outliers below the interspecific regression line and winter specimens fell within the range of variation of other species, hinting that seasonal skin thickening could be renamed seasonal skin thinning in this species. Finally, a link between ecology and skin thickness was tested using the 10 species from previous analyses and data from the literature. At a phylogenetially broad scale, body size explained a greater amount of the variation in environmental parameters than skin thickness. At smaller taxonomic scales, skin thickness appears more closely linked with ecology. It is concluded that amphibians generally follow an allometric trend for skin thickness and when faced with suboptimal conditions over…
Subjects/Keywords: amphibians; skin anatomy; ecomorphology; histology; anura
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
VanBuren, C. (2017). Assessing sources of variation in amphibian skin thickness: ecological and evolutionary implications. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/267899
Chicago Manual of Style (16th Edition):
VanBuren, Collin. “Assessing sources of variation in amphibian skin thickness: ecological and evolutionary implications.” 2017. Doctoral Dissertation, University of Cambridge. Accessed January 18, 2021.
https://www.repository.cam.ac.uk/handle/1810/267899.
MLA Handbook (7th Edition):
VanBuren, Collin. “Assessing sources of variation in amphibian skin thickness: ecological and evolutionary implications.” 2017. Web. 18 Jan 2021.
Vancouver:
VanBuren C. Assessing sources of variation in amphibian skin thickness: ecological and evolutionary implications. [Internet] [Doctoral dissertation]. University of Cambridge; 2017. [cited 2021 Jan 18].
Available from: https://www.repository.cam.ac.uk/handle/1810/267899.
Council of Science Editors:
VanBuren C. Assessing sources of variation in amphibian skin thickness: ecological and evolutionary implications. [Doctoral Dissertation]. University of Cambridge; 2017. Available from: https://www.repository.cam.ac.uk/handle/1810/267899
University of Gothenburg / Göteborgs Universitet
24.
Månsson, Jan-Eric, 1946-.
Structure and distribution of gangliosides in human tissues.
Degree: 1974, University of Gothenburg / Göteborgs Universitet
URL: http://hdl.handle.net/2077/13124
Subjects/Keywords: Gangliosides: anatomy & histology
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Månsson, Jan-Eric, 1. (1974). Structure and distribution of gangliosides in human tissues. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/13124
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Månsson, Jan-Eric, 1946-. “Structure and distribution of gangliosides in human tissues.” 1974. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed January 18, 2021.
http://hdl.handle.net/2077/13124.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Månsson, Jan-Eric, 1946-. “Structure and distribution of gangliosides in human tissues.” 1974. Web. 18 Jan 2021.
Vancouver:
Månsson, Jan-Eric 1. Structure and distribution of gangliosides in human tissues. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 1974. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/2077/13124.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Månsson, Jan-Eric 1. Structure and distribution of gangliosides in human tissues. [Thesis]. University of Gothenburg / Göteborgs Universitet; 1974. Available from: http://hdl.handle.net/2077/13124
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
25.
VanBuren, Collin.
Assessing sources of variation in amphibian skin thickness : ecological and evolutionary implications.
Degree: PhD, 2017, University of Cambridge
URL: https://doi.org/10.17863/CAM.13824
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.725579
► The skin is the largest organ of the body and provides many functions. Among tetrapod vertebrates, amphibian skin is semi-permeable and responsible for a greater…
(more)
▼ The skin is the largest organ of the body and provides many functions. Among tetrapod vertebrates, amphibian skin is semi-permeable and responsible for a greater proportion of water absorption and gas exchange. Myriad factors affect the physiological performance of amphibian skin. Morphological traits linked with amphibian skin physiology or ecology have remained difficult to discern because of a lack of quantitative comparative research and the discovery of sources of intraspecific variation that are mostly ignored in study designs. This thesis aims to address the effects of these sources of variation using a trait that is known to vary between sexes, among seasons, and among body regions and thought to be linked with physiology or ecology, skin thickness. The first source of variation addressed is sexual dimorphism. Specimens of the white-lipped treefrog, Litoria infrafrenata, that display sexual dimorphism in body size and skin thickness were used to test if body size was the main determinate of sexually dimorphic skin thickness. Size corrected values did not significantly differ between males and females, although the sample size was small. Seasonal variation in skin thickness has also been documented in some species, so the American bullfrog (Lithobates catesbeianus), the Northern leopard frog (L. pipiens), and the spring peeper (Pseudacris crucifer) from multiple months of the year were sampled to determine if skin thickness increased in the autumn or winter months. Seasonal skin thickening was only detected in L. catesbeianus, and skin from autumn and winter was significantly thicker than from earlier in the year. This pattern was also detectable in museum specimens collected 80 years ago, although the signal was damped, possibly due to preservation. Using a dataset of 10 species and published data, a general pattern was uncovered whereby the dorsal skin is the thickest region and the ventral thigh region is the thinnest. However, this pattern is not always true for every individual of every species (L. pipiens and P. crucifer) and in some species the dorsal skin is thinnest (Bokermannohyla alvarengai and Litoria infrafrenata). The same dataset found that skin thickness is significantly related to body size, as was found in the chapter on Litoria infrafrenata. Summer specimens of Lithobates catesbeianus were outliers below the interspecific regression line and winter specimens fell within the range of variation of other species, hinting that seasonal skin thickening could be renamed seasonal skin thinning in this species. Finally, a link between ecology and skin thickness was tested using the 10 species from previous analyses and data from the literature. At a phylogenetially broad scale, body size explained a greater amount of the variation in environmental parameters than skin thickness. At smaller taxonomic scales, skin thickness appears more closely linked with ecology. It is concluded that amphibians generally follow an allometric trend for skin thickness and when faced with suboptimal conditions over…
Subjects/Keywords: 597.8; amphibians; skin anatomy; ecomorphology; histology; anura
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APA (6th Edition):
VanBuren, C. (2017). Assessing sources of variation in amphibian skin thickness : ecological and evolutionary implications. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.13824 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.725579
Chicago Manual of Style (16th Edition):
VanBuren, Collin. “Assessing sources of variation in amphibian skin thickness : ecological and evolutionary implications.” 2017. Doctoral Dissertation, University of Cambridge. Accessed January 18, 2021.
https://doi.org/10.17863/CAM.13824 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.725579.
MLA Handbook (7th Edition):
VanBuren, Collin. “Assessing sources of variation in amphibian skin thickness : ecological and evolutionary implications.” 2017. Web. 18 Jan 2021.
Vancouver:
VanBuren C. Assessing sources of variation in amphibian skin thickness : ecological and evolutionary implications. [Internet] [Doctoral dissertation]. University of Cambridge; 2017. [cited 2021 Jan 18].
Available from: https://doi.org/10.17863/CAM.13824 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.725579.
Council of Science Editors:
VanBuren C. Assessing sources of variation in amphibian skin thickness : ecological and evolutionary implications. [Doctoral Dissertation]. University of Cambridge; 2017. Available from: https://doi.org/10.17863/CAM.13824 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.725579
26.
Gunnell, Clayton F.
Anatomical Complications Related to Dental Implant Surgery.
Degree: M.S. in Oral Biology, Oral Biology (graduate program), 2013, Creighton University
URL: http://hdl.handle.net/10504/42731
► With the advancement of dental surgical procedures, it is critical that the dental surgeon identify the anatomy surrounding the surgical area. The anatomy of the…
(more)
▼ With the advancement of dental surgical procedures, it is critical that the dental surgeon identify the
anatomy surrounding the surgical area. The
anatomy of the oral cavity and surrounding regions become extremely important to dentists for successful placement of dental implants. Dental implants are becoming more popular and are commonly placed by specialists and dentists around the world. The purpose of this thesis is to help identify the
anatomy of the oral cavity and surrounding regions so potential complications can be minimized during implant surgery. The focus will be primarily on the maxillary artery and its branches, maxillary division of trigeminal nerve (CN V2), mandibular division of trigeminal nerve (CN V3), and several muscles associated with the oral cavity. Cadaveric photographs and cone beam computed tomography scans (CBCT) are used to help demonstrate the pathway of arteries and nerves within the osseous
anatomy. CBCT scans will help clinicians have a better understanding of the anatomical structures when performing dental procedures specifically dental implants. Thorough understanding of the
anatomy gives the dentist the confidence to restore function, esthetics, and health to their patients. This thesis does not attempt to discuss every blood vessel, nerve, and muscle found in the oral cavity; however, it does focus on the typical pathway of oral
anatomy that could be encountered during dental implant surgery.
Advisors/Committee Members: Norton, Neil S. (advisor), Gunnell, Clayton F. (cuauthor).
Subjects/Keywords: Dental Implants; Dental Implants – adverse effects; Maxillary Artery – anatomy & histology; Mouth – anatomy & histology
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APA (6th Edition):
Gunnell, C. F. (2013). Anatomical Complications Related to Dental Implant Surgery. (Masters Thesis). Creighton University. Retrieved from http://hdl.handle.net/10504/42731
Chicago Manual of Style (16th Edition):
Gunnell, Clayton F. “Anatomical Complications Related to Dental Implant Surgery.” 2013. Masters Thesis, Creighton University. Accessed January 18, 2021.
http://hdl.handle.net/10504/42731.
MLA Handbook (7th Edition):
Gunnell, Clayton F. “Anatomical Complications Related to Dental Implant Surgery.” 2013. Web. 18 Jan 2021.
Vancouver:
Gunnell CF. Anatomical Complications Related to Dental Implant Surgery. [Internet] [Masters thesis]. Creighton University; 2013. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10504/42731.
Council of Science Editors:
Gunnell CF. Anatomical Complications Related to Dental Implant Surgery. [Masters Thesis]. Creighton University; 2013. Available from: http://hdl.handle.net/10504/42731
University of British Columbia
27.
Vogl, Adalbert Wayne.
Comparative and functional anatomy of cerebrally related retial systems in the family Monodontidae (order Cetacea).
Degree: PhD, Zoology, 1979, University of British Columbia
URL: http://hdl.handle.net/2429/23910
► In this study, I consider the comparative and functional anatomy of cerebrally related retial systems in the two constituent species (Monodon monoceros and Delphinapterus leucas)…
(more)
▼ In this study, I consider the comparative and functional anatomy of cerebrally related retial systems in the two constituent species (Monodon monoceros and Delphinapterus leucas) of the family Monodontidae (order Cetacea).
The internal carotid arteries, the "classical" vessels of cerebral supply in vertebrates, are completely non-functional as cerebral supply vessels in the Monodontidae. Moreover, there are no other channels that contribute directly to intracranial supply. Rather, the brain, or more precisely, the entire central nervous system, is vascularized indirectly via an extensive arterial plexus or rete mirabile. This plexus is found in the thorax, lumbar region, neural canal and cranium. Vessels that contribute to retial formation are numerous and include those which in other mammals contribute directly to supply of the central nervous system and/or its membranes. Efferent retial vessels are few and include two pairs of subdural intracranial trunks that supply the brain, and numerous small segmental vessels that penetrate the spinal dura and vascularize the spinal cord.
Subdural arterial circulation in the Monodontidae is modified after the basic mammalian pattern. Within the cranium, it is characterized by: (1) an incomplete circle of Willis (due to (a) independence of the anterior cerebral arteries and (b) the lack of anastomoses between the two pairs of
trunks which take origin from the rete),
(2) extensive cortical supply by the anterior choroid arteries, and
(3) absence of a vertebral basilar system.
Subdural arteries coursing to the spinal cord do so mainly between successive ventral spinal roots. An A. radicularis magna is not evident, nor are anterior or posterior spinal arteries. Hence, there are differences between the subdural circulatory patterns in the Monodontidae and those in other mammals, however the major site of vascular modification is epidural with formation of the rete mirabile.
Though gross retial anatomy is the same in Monodon monoceros and Delphinapterus leucas, and is generally similar to that described for other odontocetes, there are two related characteristics that appear species specific: thoracic retial size and the number of intercostal spaces supplied by the supreme intercostal arteries. Both are larger in Monodon monoceros, as are hematological values (hematocrit and hemoglobin concentration) which, in this study, are used as indices of diving ability. These data are consistent with the hypothesis that cerebral related retia in the Cetacea are related to the diving habit.
Microscopically, the rete generally consists of small muscular arteries embedded in fatty connective tissue interlaced with a few nerve trunks and veins. Arterial walls are characterized by a distinct internal elastic lamella, a tunica media of 12-14 layers of vascular smooth muscle, and an adventitia of alternating layers of collagen and elastin. Retial
arteries are at best poorly innervated.
The substructure of retial arteries resembles that of other mammalian arteries except for the presence of…
Subjects/Keywords: Cetacea - anatomy & histology; Cetacea - Anatomy
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APA (6th Edition):
Vogl, A. W. (1979). Comparative and functional anatomy of cerebrally related retial systems in the family Monodontidae (order Cetacea). (Doctoral Dissertation). University of British Columbia. Retrieved from http://hdl.handle.net/2429/23910
Chicago Manual of Style (16th Edition):
Vogl, Adalbert Wayne. “Comparative and functional anatomy of cerebrally related retial systems in the family Monodontidae (order Cetacea).” 1979. Doctoral Dissertation, University of British Columbia. Accessed January 18, 2021.
http://hdl.handle.net/2429/23910.
MLA Handbook (7th Edition):
Vogl, Adalbert Wayne. “Comparative and functional anatomy of cerebrally related retial systems in the family Monodontidae (order Cetacea).” 1979. Web. 18 Jan 2021.
Vancouver:
Vogl AW. Comparative and functional anatomy of cerebrally related retial systems in the family Monodontidae (order Cetacea). [Internet] [Doctoral dissertation]. University of British Columbia; 1979. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/2429/23910.
Council of Science Editors:
Vogl AW. Comparative and functional anatomy of cerebrally related retial systems in the family Monodontidae (order Cetacea). [Doctoral Dissertation]. University of British Columbia; 1979. Available from: http://hdl.handle.net/2429/23910
University of Edinburgh
28.
Douvaras, Panagiotis.
Effects of age and Pax6 deficiency on mouse limbal stem cell function.
Degree: PhD, 2010, University of Edinburgh
URL: http://hdl.handle.net/1842/4414
► The conventional view for corneal epithelial maintenance suggests that a stem cell population found in the limbus (at the rim of the cornea) produces daughter…
(more)
▼ The conventional view for corneal epithelial maintenance suggests that a stem cell population found in the limbus (at the rim of the cornea) produces daughter cells, called transient amplifying cells, which migrate centripetally. This limbal stem cell (LSC) hypothesis was recently questioned and the alternative model suggests that stem cells are present throughout the corneal epithelium. The main aims of this thesis were to investigate whether age and Pax6 genotype affect LSC function. Previous work with X-inactivation mosaics revealed radial stripes of β-galactosidase-expressing cells in the corneal epithelium (from about 5 weeks of age), which decreased with age and were reduced in Pax6+/- mice (a model for aniridia, a human eye disease). The reduction in Pax6+/- mice could be due to either reduced LSCs function or a more coarse-grained mosaicism caused by reduced cell mixing during development. Comparison of patch sizes in Pax6+/- and wild-type X-inactivation mosaics showed that patches were smaller in Pax6+/- cornea epithelia before the initiation of stripes (3 weeks of age). This implies that stripe-number reduction is not caused by reduced cell mixing, so an effect on LSC function remained a possibility. Thus, the numbers of label-retaining cells (putative stem cells) in Pax6+/- were compared to controls at 15 and 30 weeks old but they were not reduced at 30 weeks or in Pax6+/- mice, as had been predicted. The failure to demonstrate the predicted result suggests either that the hypothesis was incorrect or the experimental approach was inappropriate. Furthermore, it was discovered that mice expressing β-galactosidase under the keratin 5 promoter produced rare stripes in the corneal epithelium, which are likely to represent clonal lineages derived from individual stem cells. Older mice demonstrated a significantly lower frequency of stripes, a result compatible with the predicted reduction of active LSC with age. Pax6+/- corneas were highly abnormal and stripes were not formed properly, so direct comparison was not possible. Finally, pilot experiments with conditional expression of a reporter gene revealed the successful formation of a stripe, and hence provide a plausible alternative approach to compare stripe numbers reflecting active LSCs but the method has yet to be optimised. Overall, the results suggest that LSCs are reduced with age and support the limbal location of stem cells maintaining the corneal epithelium.
Subjects/Keywords: 617.7; corneal epithelial maintenance; limbal stem cell hypothesis; corneal epithelium; Pax6+/-; agre related
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APA (6th Edition):
Douvaras, P. (2010). Effects of age and Pax6 deficiency on mouse limbal stem cell function. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/4414
Chicago Manual of Style (16th Edition):
Douvaras, Panagiotis. “Effects of age and Pax6 deficiency on mouse limbal stem cell function.” 2010. Doctoral Dissertation, University of Edinburgh. Accessed January 18, 2021.
http://hdl.handle.net/1842/4414.
MLA Handbook (7th Edition):
Douvaras, Panagiotis. “Effects of age and Pax6 deficiency on mouse limbal stem cell function.” 2010. Web. 18 Jan 2021.
Vancouver:
Douvaras P. Effects of age and Pax6 deficiency on mouse limbal stem cell function. [Internet] [Doctoral dissertation]. University of Edinburgh; 2010. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1842/4414.
Council of Science Editors:
Douvaras P. Effects of age and Pax6 deficiency on mouse limbal stem cell function. [Doctoral Dissertation]. University of Edinburgh; 2010. Available from: http://hdl.handle.net/1842/4414
Michigan State University
29.
Skrypec, Daniel J.
Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism.
Degree: MS, Department of Food Science and Human Nutrition, 1981, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:36439
Subjects/Keywords: Colon (Anatomy); Epithelium; Cytology; Rats
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APA (6th Edition):
Skrypec, D. J. (1981). Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:36439
Chicago Manual of Style (16th Edition):
Skrypec, Daniel J. “Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism.” 1981. Masters Thesis, Michigan State University. Accessed January 18, 2021.
http://etd.lib.msu.edu/islandora/object/etd:36439.
MLA Handbook (7th Edition):
Skrypec, Daniel J. “Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism.” 1981. Web. 18 Jan 2021.
Vancouver:
Skrypec DJ. Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism. [Internet] [Masters thesis]. Michigan State University; 1981. [cited 2021 Jan 18].
Available from: http://etd.lib.msu.edu/islandora/object/etd:36439.
Council of Science Editors:
Skrypec DJ. Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism. [Masters Thesis]. Michigan State University; 1981. Available from: http://etd.lib.msu.edu/islandora/object/etd:36439
University of Arizona
30.
Jimenez, Desmond Rito.
Ultrastructure and function of the ventriculus of the honey bee, Apis mellifera.
Degree: 1987, University of Arizona
URL: http://hdl.handle.net/10150/184266
► The ventricular epithelia of adult worker honey bees were investigated biochemically and ultrastructurally. The midgut tissues were shown to produce an endoprotease with trypsin-like activity.…
(more)
▼ The ventricular epithelia of adult worker honey bees were investigated biochemically and ultrastructurally. The midgut tissues were shown to produce an endoprotease with trypsin-like activity. Enzyme activity was highest in the midgut tissues and the ectoperitrophic space of free-flying honey bees and of caged bees fed pollen. Lower levels of activity occurred in caged bees restricted to sucrose or fed artificial diets. The trypsin-like activity declined as the protein intake of the bees decreased with age. Ultrastructural studies revealed columnar cells in the posterior midgut engaged in the synthesis and release of membrane-bound vesicles. The apical cytoplasm of the epithelial cells in this region contains numerous electron dense vesicles which are released into the ectoperitrophic space of the midgut lumen. The microvilli in the crypts of this region are short, branching, and microvesiculate. Throughout the remainder of the midgut, the microvilli are profuse and elongate. The presence of the endogenously produced endoprotease and the regional variation in cell ultrastructure suggest that the honey bee may rely on countercurrent flow to distribute enzymes and nutrients efficiently throughout the midgut. Ultrastructural cytochemistry localized acid phosphatase and nonspecific esterase activity in primary and secondary lysosomes dispersed throughout the midgut tissues. Alkaline phosphatase activity was localized within large electron lucent microbodies that are present in all midgut columnar cells. The peroxisomal marker enzymes, catalase and L-α-hydroxy acid oxidase, were also localized in the same microbodies which previously had been described as holocrine secretory granules involved in dietary mineral regulation. Morphological and cytochemical assays suggest that the holocrine secretory granule arises from a microperoxisomal compartment involved in intermediary metabolism in the midgut of adult honey bees.
Advisors/Committee Members: Gilliam, Marha (advisor), Berry, James (committeemember), Sinclair, Norval (committeemember), McCaughey, William (committeemember), Nutting, William (committeemember).
Subjects/Keywords: Honeybee – Physiology.;
Honeybee – Anatomy.;
Epithelium.
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APA ·
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APA (6th Edition):
Jimenez, D. R. (1987). Ultrastructure and function of the ventriculus of the honey bee, Apis mellifera.
(Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/184266
Chicago Manual of Style (16th Edition):
Jimenez, Desmond Rito. “Ultrastructure and function of the ventriculus of the honey bee, Apis mellifera.
” 1987. Doctoral Dissertation, University of Arizona. Accessed January 18, 2021.
http://hdl.handle.net/10150/184266.
MLA Handbook (7th Edition):
Jimenez, Desmond Rito. “Ultrastructure and function of the ventriculus of the honey bee, Apis mellifera.
” 1987. Web. 18 Jan 2021.
Vancouver:
Jimenez DR. Ultrastructure and function of the ventriculus of the honey bee, Apis mellifera.
[Internet] [Doctoral dissertation]. University of Arizona; 1987. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10150/184266.
Council of Science Editors:
Jimenez DR. Ultrastructure and function of the ventriculus of the honey bee, Apis mellifera.
[Doctoral Dissertation]. University of Arizona; 1987. Available from: http://hdl.handle.net/10150/184266
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Open Access Theses and Dissertations
