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NSYSU

1. Sy, Wei-Dih. Analysis of human Dynamin IV (Dymple) gene promoter.

Degree: Master, Biological Sciences, 2003, NSYSU

We first identified the transcriptional regulatory element of the human dynamin IV gene (Hdyn IV; dymple). The Hdyn IV belongs to a large GTPase family. This protein has a N-terminal highly conserved tripartite GTP-binding domain, coiled-coil (CC) region, but it lacks the pleckstrin homology (PH) domain and a modestly conserved C-terminal proline rich domain (PRD). Hdyn IV gene is enriched in subcellular membrane fractions of cytoplasmic vesicles and endoplasmic reticulum, and the function of Hdyn IV gene is considered to be associated with the functions of mitochondria. The Hdyn IV is expressed as four alternative splicing variants in all eukaryotic organisms. Our question concerning why expressions of four alternative splicing variants in brain tumor tissues? To elucidate the regulatory mechanism and the transcription factors involved, we firstly determined the transcriptional start site by 5â RACE. We next cloned the 5â-flanking region of the Hdyn IV gene and determined the nucleotide sequence of 999 bases upstream from the transcription start site. The promoter has several potential binding sites for AP2, Sp1 binding protein, but it lacks TATA and CAAT boxes. Transfection studies using a series of Hdyn IV promoter luciferase constructs in HeLa cell demonstrate that the 5âflanking region has a promoter activity. Functional promoter element of the Hdyn IV gene was located within the â140~ +29 region. Deletion analyses demonstrated that the minimal promoter activity for the transcriptional element of Hdyn IV was detected in the sequence between nucleotides â110 and â100. Electorphoretic mobility shift assay demonstrated that a putative transcriptional factor bound to the â119 to â90 region. Site-directed mutagenesis analysis of this region revealed that nucleotides at positions â108 to â100 were essential for transactivation mediated by this element. To summary, the data indicated that the ââCTCCCAGCAââ (-108~ -100) sequence is capable of regulating Hdyn IV gene expression. However, the protein involved in the binding of this novel sequence requires further study. Advisors/Committee Members: Hsu, Ching-Mei (chair), Hong, Yi-Ren (committee member), Cheng, Jiin-Tsuey (chair), Cho, Chung-Lung (committee member).

Subjects/Keywords: transcription factor; promoter; DynIV

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APA (6th Edition):

Sy, W. (2003). Analysis of human Dynamin IV (Dymple) gene promoter. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904103-145445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Sy, Wei-Dih. “Analysis of human Dynamin IV (Dymple) gene promoter.” 2003. Thesis, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904103-145445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Sy, Wei-Dih. “Analysis of human Dynamin IV (Dymple) gene promoter.” 2003. Web. 05 Mar 2021.

Vancouver:

Sy W. Analysis of human Dynamin IV (Dymple) gene promoter. [Internet] [Thesis]. NSYSU; 2003. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904103-145445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sy W. Analysis of human Dynamin IV (Dymple) gene promoter. [Thesis]. NSYSU; 2003. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904103-145445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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