Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for subject:(Dual electrochemical optical). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of New South Wales

1. Chockalingam, Muthukumar. Development of cell based biosensor with dual detection using electrochemical impedance spectroscopy and optical fluorescence microscopy.

Degree: Chemistry, 2012, University of New South Wales

In a normal environment, cells are subject to multiple signals such as secretion of proteins from neighboring cells and biochemical interactions with the extracellular matrix (ECM). Specifically, Arg-Gly-Asp-Cys (RGDC) ligands present in ECM has been known to bind to cellular integrins, thereby regulating cell adhesion and signaling pathways. Investigating the link between cell surface interactions and G-protein coupled receptor activation is crucial to understand cancer and various disease mechanisms. The dual electrochemical and optical investigation can provide comprehensive information about these networks as electrochemistry can monitor minute changes in morphologies as well as biochemical and ionic changes while optical microscopy has the potential to monitor specific cell signaling processes as well as morphologic changes. The aim of this work is two fold 1) present RGDC ligands on transparent Indium tin oxide (ITO) electrodes in a controlled manner and 2) monitor the G-protein coupled receptor (GPCR) activation signals on varied cell surface interactions using combined fluorescence microscopy and electrochemical impedance spectroscopy. To achieve this, 1) the surface were modified with phosphonate based self-assembled monolayers (SAMs) so that adhesion and electron transfer properties of the electrode is well controlled, 2) a mixture of cell adhesive GRGDC ligands and antifouling molecules were covalently coupled on top of the monolayer to control cell adhesion, 3) finally, simultaneous live cell fluorescence imaging and electrochemical impedance spectroscopy was conducted on the prepared surfaces to analyze GPCR activation (calcium signals) and their link with cell adhesion. The results showed stable and well packed phosphonohexadecanoic acid SAMs formed only on smooth amorphous ITO surfaces. Stochastic optical reconstruction (STORM) single molecular fluorescence imaging of ITO surfaces showed well distributed RGDC-Alexa Fluor 647 molecules on modified ITO surfaces. The cell adhesion and spreading was found maximum on 1:103 (GRGDC:antifouling) surface and minimum on 1:109 surfaces. The dual optical electrochemical investigations showed that GPCR activation of cells attached on 1:106 and 1:109 surfaces showed large increase in impedance (morphological changes) whereas 1:103 and fibronectin coated surfaces showed maximum calcium signals. This dual investigations allow comprehensive understanding of cell signaling processes, fluorescence microscopy provides minute changes in calcium flux, while impedance spectroscopy providing information about minute changes in morphologies. Advisors/Committee Members: Gooding, Justin, Chemistry, Faculty of Science, UNSW, Gaus, Katharina, Centre for Vascular Research, Faculty of Medicine, UNSW.

Subjects/Keywords: Dual electrochemical optical; Cell based biosensors; Indium tin oxide

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Chockalingam, M. (2012). Development of cell based biosensor with dual detection using electrochemical impedance spectroscopy and optical fluorescence microscopy. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52059 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10729/SOURCE01?view=true

Chicago Manual of Style (16th Edition):

Chockalingam, Muthukumar. “Development of cell based biosensor with dual detection using electrochemical impedance spectroscopy and optical fluorescence microscopy.” 2012. Doctoral Dissertation, University of New South Wales. Accessed October 24, 2020. http://handle.unsw.edu.au/1959.4/52059 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10729/SOURCE01?view=true.

MLA Handbook (7th Edition):

Chockalingam, Muthukumar. “Development of cell based biosensor with dual detection using electrochemical impedance spectroscopy and optical fluorescence microscopy.” 2012. Web. 24 Oct 2020.

Vancouver:

Chockalingam M. Development of cell based biosensor with dual detection using electrochemical impedance spectroscopy and optical fluorescence microscopy. [Internet] [Doctoral dissertation]. University of New South Wales; 2012. [cited 2020 Oct 24]. Available from: http://handle.unsw.edu.au/1959.4/52059 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10729/SOURCE01?view=true.

Council of Science Editors:

Chockalingam M. Development of cell based biosensor with dual detection using electrochemical impedance spectroscopy and optical fluorescence microscopy. [Doctoral Dissertation]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/52059 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10729/SOURCE01?view=true

.