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You searched for subject:(Drosophila metabolism 60). Showing records 1 – 3 of 3 total matches.

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1. Balasubramani, Anand. Evolutionarily Conserved Cis-Acting Elements Regulate Lineage-Specific Expression Of Ifng.

Degree: PhD, 2010, University of Alabama – Birmingham

The ability to differentially manipulate available genetic information in order to generate diverse cellular identities represents an innovation of complex multicellular eukaryotic organisms. Cis-acting modules that regulate transcription play extremely important roles in ensuring lineage-specific expression of genes that define cellular identities. In our studies, using Interferon-gamma (IFN-gamma, a cytokine encoded by the gene Ifng), a cytokine encoded by T, NK and NKT cells as a model, we have identified and characterized the roles of key cis regulatory elements that facilitate T lineage specific induction as well as repression of Ifng transcription. In particular, our studies have highlighted the role of RelA, an NF-kappa B family member in driving acute Ifng transcription in response to reactivation signals. These studies have led to the recognition of multiple conserved non-coding sequences (CNS) in the Ifng locus including CNSs -34, -22, +46 and +54 that positively modulate Ifng transcription by co-recruiting STAT4 and RelA. To gain further insight into the actions of these complex cis-regulatory modules in induction of Ifng transcription in vivo, we have generated mice that carry a targeted deletion of a candidate enhancer CNS-22 that has been demonstrated to recruit multiple key trans factors including T-bet, STAT4, Runx3 and RelA to transactivate Ifng transcription. Th1, Tc1 and NK cells derived from CNS-22-/- mice are severely compromised in their ability to secrete IFN-gamma in response to IL-12 and IL-18, but also show less prominent defects in IFN-gamma induction in response to TCR reactivation signals as well. Using CNS-22 as a candidate enhancer, we have also explored some of the fundamental mechanisms that facilitate enhancer actions of CNS-22. Specifically, we demonstrate that activation induced local hyperacetylation of regions that flank CNS-22 plays an essential role in driving inducible transcription of Ifng. Lastly, we extend our findings to other distal enhancers and demonstrate stimulus-responsive activation of Ifng expression is associated with inducible hyperacetylation of local micro-domains that flank multiple enhancers that activate Ifng transcription.

PhD

1 online resource (x, 114 p.) :ill., digital, PDF file.

Microbiology

Joint Health Sciences

cytokine enhancers epigenetics gene regulation helper T cell Th1 cell

UNRESTRICTED

Advisors/Committee Members: Casey T. Weaver, Hatton, Robin D. Klug, Christopher A. Benveniste, Etty N. Ryan, Thomas M. Justement, Louis B..

Subjects/Keywords: DNA Replication – physiology.<; br>; Drosophila – metabolism.<; br>; Drosophila Proteins – metabolism.<; br>; GTP Phosphohydrolases – metabolism.<; br>; Microfilament Proteins – metabolism.<; br>; Multiprotein Complexes – metabolism.<; br>; Origin Recognition Complex – metabolism.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Balasubramani, A. (2010). Evolutionarily Conserved Cis-Acting Elements Regulate Lineage-Specific Expression Of Ifng. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,1428

Chicago Manual of Style (16th Edition):

Balasubramani, Anand. “Evolutionarily Conserved Cis-Acting Elements Regulate Lineage-Specific Expression Of Ifng.” 2010. Doctoral Dissertation, University of Alabama – Birmingham. Accessed April 03, 2020. http://contentdm.mhsl.uab.edu/u?/etd,1428.

MLA Handbook (7th Edition):

Balasubramani, Anand. “Evolutionarily Conserved Cis-Acting Elements Regulate Lineage-Specific Expression Of Ifng.” 2010. Web. 03 Apr 2020.

Vancouver:

Balasubramani A. Evolutionarily Conserved Cis-Acting Elements Regulate Lineage-Specific Expression Of Ifng. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2010. [cited 2020 Apr 03]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,1428.

Council of Science Editors:

Balasubramani A. Evolutionarily Conserved Cis-Acting Elements Regulate Lineage-Specific Expression Of Ifng. [Doctoral Dissertation]. University of Alabama – Birmingham; 2010. Available from: http://contentdm.mhsl.uab.edu/u?/etd,1428

2. Jumbo Lucioni, Patricia P. A system genetics analysis of energy metabolism traits in Drosophila melanogaster.

Degree: PhD, 2009, University of Alabama – Birmingham

Obesity is emerging as a global public health problem and it has shown to precede and predict the development of type 2 diabetes, a complex disease that has also reached epidemic proportions in the US and worldwide. Despite that obesity-related traits are highly heritable, the genetic basis underlying their natural variation and the loci playing pleiotropic roles among organismal traits have not been fully elucidated. The overall goals of these present studies were: to shed light on the architecture of the genetic coexpression networks regulating variations in obesity-related traits, elucidate the extent to which they are regulated by pleiotropic loci, and identify pleiotropic alleles between metabolism and life-history traits to provide key insights into why different alleles are allowed to persist in natural populations, despite the fact that some of them confer susceptibility to metabolic disorders. Using a wild-derived population of Drosophila melanogaster as model system, our results highlighted the relevance of non-metabolic pathways such as immune response, neurogenesis and neuronal function, cell growth, food processing and water balance as key regulators of organismal body weight, metabolic rate and body composition traits. Differential expression of cycling/photoperiodic genes among young adult flies underlies the genetic forces shaping phenotypic variation in mitochondrial bioenergetic traits. Furthermore, the elucidation of pleiotropic transcriptional modules provided a key insight into the molecular basis of the well established trade-offs between body weight, reproduction, and survival of food deprivation. Our data further indicate that molecular regulation of mitochondrial respiration plays a critical role in mediating life history trade-offs in natural populations. In conclusion, our results confirm that the genetic basis of natural variations in obesityrelated traits involves highly interactive co-regulated transcriptional networks, and identify various pleiotropic loci underlying evolutionarily conserved trade-offs among organismal survival and reproduction which account for the perpetuation of alleles that confer susceptibility to metabolic disorders among individuals from a natural population.

1 online resource (xii, 244 p. : ill., digital, PDF file)

Nutrition Sciences;

Health Professions;

obesity-related traits Drosophila melanogaster genetic networks lifehistory traits trade-off syndecan

UNRESTRICTED

Advisors/Committee Members: De Luca, Maria, Bailey, Shannon M.<br>, Ballinger, Scott W.<br>, Garvey, W. Timothy<br>, Piyathilake, Chandrika J..

Subjects/Keywords: Drosophila melanogaster  – genetics<; br>; Energy Metabolism<; br>; Mitochondria  – metabolism<; br>; Obesity  – genetics<; br>; Obesity  – complications

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Jumbo Lucioni, P. P. (2009). A system genetics analysis of energy metabolism traits in Drosophila melanogaster. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,568

Chicago Manual of Style (16th Edition):

Jumbo Lucioni, Patricia P. “A system genetics analysis of energy metabolism traits in Drosophila melanogaster.” 2009. Doctoral Dissertation, University of Alabama – Birmingham. Accessed April 03, 2020. http://contentdm.mhsl.uab.edu/u?/etd,568.

MLA Handbook (7th Edition):

Jumbo Lucioni, Patricia P. “A system genetics analysis of energy metabolism traits in Drosophila melanogaster.” 2009. Web. 03 Apr 2020.

Vancouver:

Jumbo Lucioni PP. A system genetics analysis of energy metabolism traits in Drosophila melanogaster. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2009. [cited 2020 Apr 03]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,568.

Council of Science Editors:

Jumbo Lucioni PP. A system genetics analysis of energy metabolism traits in Drosophila melanogaster. [Doctoral Dissertation]. University of Alabama – Birmingham; 2009. Available from: http://contentdm.mhsl.uab.edu/u?/etd,568

3. Kim, Nam Chul. Identification and characterization of downstream target genes of the BMP signaling pathway.

Degree: PhD, 2010, University of Alabama – Birmingham

During nervous system development, neurons proliferate, differentiate, project their axons to their targets and make synapses with them. At the neuromuscular junction of Drosophila, these functional synapses enlarge as the body grows to maintain physiologically effective synaptic transmission with the muscles. Drosophila Bone Morphogenetic Protein (BMP) type II receptor, Wishful thinking (Wit), is important in this proper synaptic growth and function. To identify transcriptional targets of Wit signaling, we performed microarray experiments comparing normal controls to wit mutants. Through this study, we found that some of the verified target genes showed isoform-specific regulation in wit mutants, and that several neuropeptide hormones were down-regulated in wit mutants. One of the most promising targets is the novel gene CG9335 that shows 2.35 fold decrease in microarrays and about 3 fold decrease by Quantitative Real time PCR (QRT-PCR) in mutants. In situ hybridization results show that CG9335 is expressed in the optic lobe proliferating center, ventral ganglion of larval CNS and eye imaginal discs. CG9335 expression is specifically eliminated in the ventral ganglion of wit mutants. Molecular characterization of CG9335 shows that it is a Ly-6 domain-containing, GPI-anchored cell surface protein. Co-expression studies by in situ hybridization strongly suggest that CG9335 is a motoneuron expressed gene. Functional characterization using a null allele revealed that CG9335 mutants have defects in spontaneous synaptic vesicle release. The significantly decreased frequency of spontaneous vesicle release of CG9335 mutants can be rescued by neuronal expression of a CG9335 transgene. Wit mutants also show a decreased frequency of spontaneous synaptic vesicle release, and the phenoptype can be partially rescued by presynaptic expression of CG9335. Through our study, we proved our tested hypothesis; the canonical BMP signaling pathway regulates downstream target genes that are important for synaptic development and function. Further validation and study of other targets identified in our microarray study will provide much more valuable information about how BMP signaling orchestrates synapse formation and function.

1 online resource (x, 137 p. : ill., digital, PDF file)

Cell Biology;

Joint Health Sciences;

BMP Motoneuron Drosophilia Synapse Neuromuscular Junction mEJP

UNRESTRICTED

Advisors/Committee Members: Marques, Guillermo, Woods, Anne C.<br>, Chang, Chenbei<br>, Miller, Michael A.<br>, Wilson, Scott M..

Subjects/Keywords: Bone Morphogenetic Proteins  – metabolism<; br>; Central Nervous System<; br>; Drosophila melanogaster<; br>; Gene Expression Regulation, Developmental<; br>; Microarray Analysis<; br>; Signal Transduction  – physiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kim, N. C. (2010). Identification and characterization of downstream target genes of the BMP signaling pathway. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,919

Chicago Manual of Style (16th Edition):

Kim, Nam Chul. “Identification and characterization of downstream target genes of the BMP signaling pathway.” 2010. Doctoral Dissertation, University of Alabama – Birmingham. Accessed April 03, 2020. http://contentdm.mhsl.uab.edu/u?/etd,919.

MLA Handbook (7th Edition):

Kim, Nam Chul. “Identification and characterization of downstream target genes of the BMP signaling pathway.” 2010. Web. 03 Apr 2020.

Vancouver:

Kim NC. Identification and characterization of downstream target genes of the BMP signaling pathway. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2010. [cited 2020 Apr 03]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,919.

Council of Science Editors:

Kim NC. Identification and characterization of downstream target genes of the BMP signaling pathway. [Doctoral Dissertation]. University of Alabama – Birmingham; 2010. Available from: http://contentdm.mhsl.uab.edu/u?/etd,919

.