Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for subject:(Directed evolution microenvironment). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Leiden University

1. Ganapathy, S. Improvisations in phototrophy. Protein engineering and functional investigation of rhodopsin proton-pumps.

Degree: 2017, Leiden University

Microbial rhodopsins are photosensitive pigments implemented in the growth and adaptation of a large population of microorganisms. These relatively simple, tunable photosystems use a molecule of retinal as a chromophore to facilitate the conversion of sunlight to chemical energy. Retinal-based phototrophy is believed to sustain the phototrophic balance of various biospheres and has several important biotechnological applications. In this thesis, we propose the use of microbial rhodopsins as an alternative photosystem in a complementary approach towards more efficient use of photons in the solar spectrum. Towards this end, we describe the adaptation of two rhodopsin proton-pumps, namely proteorhodopsin and Gloeobacter rhodopsin, to shift their action spectrum into the near-infrared region. Several red-shifted variants of were generated by utilizing a combination of retinal analogs with specific opsin mutations. We also constructed a novel directed-evolution set-up, which allows us to generate a library of red-shifted mutants with simultaneous screening for spectral shifts and proton-pumping ability. Finally, the impact of a detergent or lipid microenvironment was tested on the various pigments generated in this study. Our results have important prospects in a number of biotechnological fields such as optogenetics, membrane-sensor technology and as a complementary photosystem for oxygenic photosynthesis. Advisors/Committee Members: Supervisor: Grip W.J. de, de, Groot H.J.M..

Subjects/Keywords: Phototrophy; Microbial rhodopsin; Proteorhodopsin; Proton pump; Spectral tuning; Retinal analog; Protein engineering; Mutagenesis; Directed evolution microenvironment; Phototrophy; Microbial rhodopsin; Proteorhodopsin; Proton pump; Spectral tuning; Retinal analog; Protein engineering; Mutagenesis; Directed evolution microenvironment

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ganapathy, S. (2017). Improvisations in phototrophy. Protein engineering and functional investigation of rhodopsin proton-pumps. (Doctoral Dissertation). Leiden University. Retrieved from http://hdl.handle.net/1887/57985

Chicago Manual of Style (16th Edition):

Ganapathy, S. “Improvisations in phototrophy. Protein engineering and functional investigation of rhodopsin proton-pumps.” 2017. Doctoral Dissertation, Leiden University. Accessed September 18, 2020. http://hdl.handle.net/1887/57985.

MLA Handbook (7th Edition):

Ganapathy, S. “Improvisations in phototrophy. Protein engineering and functional investigation of rhodopsin proton-pumps.” 2017. Web. 18 Sep 2020.

Vancouver:

Ganapathy S. Improvisations in phototrophy. Protein engineering and functional investigation of rhodopsin proton-pumps. [Internet] [Doctoral dissertation]. Leiden University; 2017. [cited 2020 Sep 18]. Available from: http://hdl.handle.net/1887/57985.

Council of Science Editors:

Ganapathy S. Improvisations in phototrophy. Protein engineering and functional investigation of rhodopsin proton-pumps. [Doctoral Dissertation]. Leiden University; 2017. Available from: http://hdl.handle.net/1887/57985

.