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You searched for subject:(Dinitrophenol treatment). Showing records 1 – 2 of 2 total matches.

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1. Schlagowski, Anna - Isabel. Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis.

Degree: Docteur es, Physiologie et biologie des organismes, populations, interactions, 2014, Université de Strasbourg

Les mécanismes impliqués dans les adaptations du phénotype métabolique musculaire au cours de l’exercice physique restent imparfaitement connus. Nous nous sommes intéressés au concept d’hormèse mitochondriale qui se définit comme un stress métabolique activant les voies de signalisation menant à une activation mitochondriale.En première partie, nous avons validé l’utilisation d’un nouveau système de mesure des échanges gazeux chez le rat au cours de différents exercices sur tapis roulant, et démontré que pour une vitesse de course sous maximale, un exercice en descente sollicite le système cardiovasculaire de façon modérée sans altérer la fonction mitochondriale musculaire, ni augmenter la production de radicaux libres oxygénés.En deuxième partie, nous avons montré qu’un découplage mitochondrial provoqué par un traitement des rats au 2,3-dinitrophénol (DNP) pendant 3 semaines engendre des adaptations métaboliques menant à l’augmentation de la masse mitochondriale du muscle squelettique. Ces animaux ont une capacité à l’exercice diminuée, malgré une augmentation de leur VO2max.Pour finir, nous avons montré qu’un préconditionnement par l’exercice protège la mitochondrie musculaire squelettique des effets délétères de l’ischémie-reperfusion. L’exercice semble activer le métabolisme via un phénomène d’hormèse mitochondriale permettant la protection musculaire. En conclusion, cette thèse nous montre d’une part l’importance de la mitochondrie (aspect quantitatif mais surtout qualitatif) en terme de limitation à l’exercice, et d’autre part nous suggère que l'optimisation du fonctionnement mitochondrial pourrait être une bonne garantie pour pouvoir lutter efficacement contre les stress, notamment oxydatifs, auxquels l'organisme est soumis en (quasi)permanence.

The mechanisms regulating the metabolic phenotype adaptations in skeletal muscle during physical exercise is still unknown.We studied the mitochondrial hormesis phenomenon that could be defined as a metabolic stress activating the signaling pathways leading to a mitochondrial stimulation (mitochondrial biogenesis).In the first part, we validated the utilization of a new system determining the gas exchange in rat during a treadmill exhaustive exercise. We showed that a submaximal downhill exercise activate moderately the cardiovascular system, without mitochondrial functional impairments and without any augmentation of the systemic ROS production. In the second part, we showed that a mitochondrial uncoupling following a dinitrophenol treatment during 3 weeks in rats induced some metabolic adaptations leading to a higher mitochondrial mass in skeletal muscle. The exercise capacity of these animals is reduced whereas the maximal oxygen consumption is higher.In the last part, we showed that a preconditioning protocol with an acute exercise protected the skeletal muscle mitochondria from the deleterious effects of ischemia-reperfusion. This exercise seems to activate the muscular metabolism via a phenomenon of mitochondrial hormesis activation, allowing an efficient…

Advisors/Committee Members: Piquard, François (thesis director).

Subjects/Keywords: Mitochondrie; Stress oxydant; Exercice; Muscle; Consommation d'oxygène; Dinitrophénol; Découplage; Skeletal muscle; Physical exercise; Mitochondria; Oxygen consumption; Dinitrophenol treatment; Oxidative stress; 573.7; 572.8

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APA (6th Edition):

Schlagowski, A. -. I. (2014). Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2014STRAJ105

Chicago Manual of Style (16th Edition):

Schlagowski, Anna - Isabel. “Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis.” 2014. Doctoral Dissertation, Université de Strasbourg. Accessed May 09, 2021. http://www.theses.fr/2014STRAJ105.

MLA Handbook (7th Edition):

Schlagowski, Anna - Isabel. “Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis.” 2014. Web. 09 May 2021.

Vancouver:

Schlagowski A-I. Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2014. [cited 2021 May 09]. Available from: http://www.theses.fr/2014STRAJ105.

Council of Science Editors:

Schlagowski A-I. Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis. [Doctoral Dissertation]. Université de Strasbourg; 2014. Available from: http://www.theses.fr/2014STRAJ105


University of New Mexico

2. Vaughan, Roger. Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity.

Degree: Individual, Family, and Community Education, 2011, University of New Mexico

Obesity is one of the most prevalent maladies in the United States and is a major cause of preventable death. Weight loss supplements frequently claim uncoupling as a mechanism of action. Uncoupling agents could be used for weight loss because they disrupt mitochondrial metabolism thereby reducing adenosine triphosphate (ATP) yield. Consequently, metabolic efficiency diminishes increasing basal metabolic rate. 2,4-Dinitrophenol (DNP) successfully uncouples but was banned in 1938 because of a narrow window between efficacy and toxicity. PURPOSE: To measure the ability of a blend that reportedly contains 17-dihydroxy-delta-5-etiocholane-7-one and p-methylcarbonylethylphenol and other substances (A7E), a purported uncoupling agent, to interfere with oxidative phosphorylation in Saccharomyces cerevisiae as evidenced by lower ATP production. METHODS: Timed culture studies of S. cerevisiae were performed using two separate agents, A7E (a purported uncoupling agent) and DNP (a known uncoupling agent) at three doses (DNP: Low, Moderate, High; A7E: Low, Moderate, High ), and an ethanol-treated control to detect interference with mitochondrial coupling. Microbial staining was used to ascertain cell viability and any changes in cell densities. ATP production was estimated by measuring luminosity generated in culture supernatants using the ATP Bioluminescence Kit (Sigma St. Louis, MO.). RESULTS: Luminosity measurements estimating ATP production revealed statistically lower ATP in agent-treated supernatants than in control supernatants (p < 0.001) except for low dose A7E versus control (p > .05), suggesting that both A7E and DNP acted by a mechanism of uncoupling. Luminosity values were measured in relative luminosity units (RLU). Treatments with A7E at Low, Moderate, and High doses generated group mean luminosity values of 24,596, 16,038, and 6,969, respectively. Treatments with DNP at Low, Moderate, and High doses generated group mean luminosity values of 17,191, 11,901, 767 RLU respectively. The control group mean was 31,645 RLU. Culture studies had no statistical difference (p > 0.0167 adjusted) in total and viable cell densities between the control and A7E and DNP treatments. CONCLUSION: A7E is effective at reducing ATP levels in this assay, as is known uncoupling agent DNP, supporting the hypothesis that A7E may also uncouple oxidative phosphorylation. Because A7E requires a higher dosage than DNP to equivalently disrupt mitochondrial metabolism, it may have a wider range of therapeutic doses. This suggests that A7E should be studied further for safety and efficacy with respect to metabolic efficiency and weight loss. Advisors/Committee Members: Conn, Carole, Lockner, Donna, Conn, Carole, Dichosa, Armand.

Subjects/Keywords: Phosphorylation.; Adenosine triphosphate.; Dinitrophenol.; Mitochondria – Physiology.; Saccharomyces cerevisiae – Metabolism.; Obesity – Treatment.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Vaughan, R. (2011). Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity. (Masters Thesis). University of New Mexico. Retrieved from http://hdl.handle.net/1928/12800

Chicago Manual of Style (16th Edition):

Vaughan, Roger. “Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity.” 2011. Masters Thesis, University of New Mexico. Accessed May 09, 2021. http://hdl.handle.net/1928/12800.

MLA Handbook (7th Edition):

Vaughan, Roger. “Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity.” 2011. Web. 09 May 2021.

Vancouver:

Vaughan R. Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity. [Internet] [Masters thesis]. University of New Mexico; 2011. [cited 2021 May 09]. Available from: http://hdl.handle.net/1928/12800.

Council of Science Editors:

Vaughan R. Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity. [Masters Thesis]. University of New Mexico; 2011. Available from: http://hdl.handle.net/1928/12800

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