subject:(Dengue virus)

University of Hawaii – Manoa

1. Hammond, Kimberly Elaine. Modulation of TNF-α production by small RNA in dengue virus infected human monocytic cells.

Degree: 2016, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100961

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M.S. University of Hawaii at Manoa 2012.

The immunopathology of dengue virus (DENV) infection is associated with increased TNF-α production. In this study, small RNA-mediated… (more)

Subjects/Keywords: dengue virus

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APA (6^th Edition):

Hammond, K. E. (2016). Modulation of TNF-α production by small RNA in dengue virus infected human monocytic cells. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hammond, Kimberly Elaine. “Modulation of TNF-α production by small RNA in dengue virus infected human monocytic cells.” 2016. Thesis, University of Hawaii – Manoa. Accessed January 20, 2021. http://hdl.handle.net/10125/100961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hammond, Kimberly Elaine. “Modulation of TNF-α production by small RNA in dengue virus infected human monocytic cells.” 2016. Web. 20 Jan 2021.

Vancouver:

Hammond KE. Modulation of TNF-α production by small RNA in dengue virus infected human monocytic cells. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10125/100961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hammond KE. Modulation of TNF-α production by small RNA in dengue virus infected human monocytic cells. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/100961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Cruz Malpica, Cristhopher Donat's. Caracterización molecular de cepas aisladas de dengue 2 en Perú; 2000-2010.

Degree: 2013, National University of San Marcos

URL: http://hdl.handle.net/20.500.12672/3674

► El virus Dengue (VDEN) es el responsable de más de 50-100 millones de casos anualmente en el mundo. La infección del dengue es causada por… (more)

Subjects/Keywords: Dengue; Virus - Aspectos genéticos; Arbovirus

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APA (6^th Edition):

Cruz Malpica, C. D. (2013). Caracterización molecular de cepas aisladas de dengue 2 en Perú; 2000-2010. (Thesis). National University of San Marcos. Retrieved from http://hdl.handle.net/20.500.12672/3674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cruz Malpica, Cristhopher Donat's. “Caracterización molecular de cepas aisladas de dengue 2 en Perú; 2000-2010.” 2013. Thesis, National University of San Marcos. Accessed January 20, 2021. http://hdl.handle.net/20.500.12672/3674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cruz Malpica, Cristhopher Donat's. “Caracterización molecular de cepas aisladas de dengue 2 en Perú; 2000-2010.” 2013. Web. 20 Jan 2021.

Vancouver:

Cruz Malpica CD. Caracterización molecular de cepas aisladas de dengue 2 en Perú; 2000-2010. [Internet] [Thesis]. National University of San Marcos; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.12672/3674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cruz Malpica CD. Caracterización molecular de cepas aisladas de dengue 2 en Perú; 2000-2010. [Thesis]. National University of San Marcos; 2013. Available from: http://hdl.handle.net/20.500.12672/3674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Evangelista Villavicencio, Julio Antonio. Caracterización de un nuevo flavivirus aislado de Culex (melanoconion) ocossa de Iquitos, Perú.

Degree: 2016, National University of San Marcos

URL: http://hdl.handle.net/20.500.12672/4885

► Describe el aislamiento y caracterización molecular de un nuevo flavivirus únicamente de mosquito, aislado de Culex (Melanoconion) occossa colectados en 2009 de un área urbana… (more)

Subjects/Keywords: Virus - Identificación; Flavivirus; Dengue

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APA (6^th Edition):

Evangelista Villavicencio, J. A. (2016). Caracterización de un nuevo flavivirus aislado de Culex (melanoconion) ocossa de Iquitos, Perú. (Thesis). National University of San Marcos. Retrieved from http://hdl.handle.net/20.500.12672/4885

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Evangelista Villavicencio, Julio Antonio. “Caracterización de un nuevo flavivirus aislado de Culex (melanoconion) ocossa de Iquitos, Perú.” 2016. Thesis, National University of San Marcos. Accessed January 20, 2021. http://hdl.handle.net/20.500.12672/4885.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Evangelista Villavicencio, Julio Antonio. “Caracterización de un nuevo flavivirus aislado de Culex (melanoconion) ocossa de Iquitos, Perú.” 2016. Web. 20 Jan 2021.

Vancouver:

Evangelista Villavicencio JA. Caracterización de un nuevo flavivirus aislado de Culex (melanoconion) ocossa de Iquitos, Perú. [Internet] [Thesis]. National University of San Marcos; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.12672/4885.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Evangelista Villavicencio JA. Caracterización de un nuevo flavivirus aislado de Culex (melanoconion) ocossa de Iquitos, Perú. [Thesis]. National University of San Marcos; 2016. Available from: http://hdl.handle.net/20.500.12672/4885

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

4. Bara, Jeffrey J., 1984-. Ecological interactions affecting the role of mosquito larvae on dengue virus transmission.

Degree: PhD, 2012, University of Louisville

URL: 10.18297/etd/70 ; https://ir.library.louisville.edu/etd/70

► In the course of their life cycle, mosquitoes undergo an ontogenetic niche shift; immature larval development occurs within an aquatic habitat from which adult mosquitoes… (more)

▼ In the course of their life cycle, mosquitoes undergo an ontogenetic niche shift; immature larval development occurs within an aquatic habitat from which adult mosquitoes subsequently disperse into the terrestrial environment. While adult female mosquitoes transmit dengue to humans, the larval stage influences dengue virus transmission significantly in several ways. The biotic and abiotic conditions in which larvae develop influence epidemiologically important aspects of adult life history and influence the geographic distribution of mosquito species. Through vertical transmission, mosquitoes may serve as reservoir hosts for dengue during periods that are unfavorable for transmission to humans. For my dissertation, I conducted research that investigated how environmental conditions experienced by larvae influence dengue transmission both directly and indirectly. In chapter 1, I experimentally infected Aedes aegypti and Aedes albopictus larvae to determine their susceptibility to dengue and evaluate the potential for horizontal transmission within the larval habitat. I found that larvae are susceptible to dengue and that horizontal transmission could be possible. In chapter 2, I tested the hypothesis that dengue infection affected mosquito fitness. The effect of infection was sex dependent; infected males took significantly longer to develop and were significantly smaller, while infection in females was cost-free. This result indicates that female larvae infected through vertical transmission are likely to be able to transmit dengue to humans as adults. In chapter 3 and 4, I investigated how resources within the larval habitat influence development and competition. Using larval detritus as a resource, I studied its utilization within the habitat, and its influence on larval development and competition. I found that larval detritus was consumed rapidly and differently from another type of invertebrate detritus. When larval detritus was the main resource within the larval habitat, Ae. albopictus significantly outcompeted Ae. aegypti; Ae. aegypti were significantly smaller and took longer to develop when reared in containers with Ae. albopictus. This result indicates that interspecific competition with Ae. albopictus significantly decreases the population growth of Ae. aegypti and may influence its ability to transmit dengue. Overall, my results further demonstrate the influential role of mosquito larvae on dengue transmission and epidemiology. Advisors/Committee Members: Remold, Susanna K..

Subjects/Keywords: Dengue virus; Larval susceptibility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bara, Jeffrey J., 1. (2012). Ecological interactions affecting the role of mosquito larvae on dengue virus transmission. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/70 ; https://ir.library.louisville.edu/etd/70

Chicago Manual of Style (16^th Edition):

Bara, Jeffrey J., 1984-. “Ecological interactions affecting the role of mosquito larvae on dengue virus transmission.” 2012. Doctoral Dissertation, University of Louisville. Accessed January 20, 2021. 10.18297/etd/70 ; https://ir.library.louisville.edu/etd/70.

MLA Handbook (7^th Edition):

Bara, Jeffrey J., 1984-. “Ecological interactions affecting the role of mosquito larvae on dengue virus transmission.” 2012. Web. 20 Jan 2021.

Vancouver:

Bara, Jeffrey J. 1. Ecological interactions affecting the role of mosquito larvae on dengue virus transmission. [Internet] [Doctoral dissertation]. University of Louisville; 2012. [cited 2021 Jan 20]. Available from: 10.18297/etd/70 ; https://ir.library.louisville.edu/etd/70.

Council of Science Editors:

Bara, Jeffrey J. 1. Ecological interactions affecting the role of mosquito larvae on dengue virus transmission. [Doctoral Dissertation]. University of Louisville; 2012. Available from: 10.18297/etd/70 ; https://ir.library.louisville.edu/etd/70

Universidade Estadual de Campinas

5. Melo, Carlos Fernando Odir Rodrigues, 1985-. Metabolômica e lipidômica dos processos infecciosos do vírus da Zika e Dengue, do mosquito ao paciente : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient .

Degree: 2019, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335891

► Abstract: Zika virus (ZIKV) is an arbovirus that plays an important role in increasing births of microcephalic babies and in adults and has been associated… (more)

▼ Abstract: Zika virus (ZIKV) is an arbovirus that plays an important role in increasing births of microcephalic babies and in adults and has been associated with Guillain-Barré syndrome and it has been of major public health concern. Dengue virus (DENV) is endemic in Brazil and, like ZIKV, is characterized by causing self-limiting infections in the vast majority of patients, being of great importance in public health due to economic losses due to decreased productivity in affected populations and above all in the hemorrhagic form of the disease that can lead to death. These two arboviruses have adapted to urban mosquitoes facilitating the occurrence of severe epidemics of Dengue and Zika since the A. aegypti (main vector) presents with gonotrophic discordance, which makes it an excellent vector. In general, mosquito-borne diseases have a tradition of being neglected, and there are gaps in important biomedical information such as mosquito infection mechanism, viral infection mechanism in man, and laboratory diagnosis. In parallel, metabolomics and lipidomics are strategic and revolutionary study methodologies that aid in the determination of important biomarkers in infection control and the global emergence of public health and the revolutionary potential of the new "omics"; which allows us to study mechanisms of infection in the adult form of the vectors in humans and is very important in the diagnosis. Given in context on screen, this project aimed to study the two most important arboviruses currently in Brazil: the ZIKV, proposing a search for biomarkers to identify and understand the mechanism of infection in the mosquito (Goal 1) and human (Goal 2) for its better disease control and development of a diagnostic method for ZIKV through "omic" platforms (Goal 3). For DENV the proposed goal was to search for biomarkers for DENV alterations in patients presenting with hemorrhagic Dengue (Goal 4) Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Catharino, Rodrigo Ramos, 1977- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Fisiopatologia Médica (nameofprogram), Müller, Karina Cogo (committee member), Kawano, Daniel Fábio (committee member), Machado, Daisy (committee member), Grotto, Rejane Maria Tommasini (committee member).

Subjects/Keywords: Zika virus; Vírus da dengue; Dengue hemorrágica; Aedes aegypti; Metabolômica; Zika virus; Dengue virus; Hemorrhagic dengue fever; Aedes argypti; Metebolomic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melo, Carlos Fernando Odir Rodrigues, 1. (2019). Metabolômica e lipidômica dos processos infecciosos do vírus da Zika e Dengue, do mosquito ao paciente : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/335891

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Melo, Carlos Fernando Odir Rodrigues, 1985-. “Metabolômica e lipidômica dos processos infecciosos do vírus da Zika e Dengue, do mosquito ao paciente : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient .” 2019. Thesis, Universidade Estadual de Campinas. Accessed January 20, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/335891.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Melo, Carlos Fernando Odir Rodrigues, 1985-. “Metabolômica e lipidômica dos processos infecciosos do vírus da Zika e Dengue, do mosquito ao paciente : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient .” 2019. Web. 20 Jan 2021.

Vancouver:

Melo, Carlos Fernando Odir Rodrigues 1. Metabolômica e lipidômica dos processos infecciosos do vírus da Zika e Dengue, do mosquito ao paciente : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient . [Internet] [Thesis]. Universidade Estadual de Campinas; 2019. [cited 2021 Jan 20]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335891.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Melo, Carlos Fernando Odir Rodrigues 1. Metabolômica e lipidômica dos processos infecciosos do vírus da Zika e Dengue, do mosquito ao paciente : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient : Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient . [Thesis]. Universidade Estadual de Campinas; 2019. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335891

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Ulanday, Gianne Eduard Limbo. Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against Dengue 2 viral protease : デング2型ウイルスプロテアーゼを標的とした新規抗ウイルス薬探索のための蛍光発色によるアッセイ法の開発.

Degree: 博士(医学), 2016, Nagasaki University / 長崎大学

URL: http://hdl.handle.net/10069/36837

► Background: Dengue disease is one of the most significant vector-borne illnesses in the world. The emergence and re-emergence of dengue infections in many parts of… (more)

Subjects/Keywords: Dengue virus; Antiviral screening; Dengue protease; NS2B-NS3pro

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ulanday, G. E. L. (2016). Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against Dengue 2 viral protease : デング2型ウイルスプロテアーゼを標的とした新規抗ウイルス薬探索のための蛍光発色によるアッセイ法の開発. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/36837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ulanday, Gianne Eduard Limbo. “Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against Dengue 2 viral protease : デング2型ウイルスプロテアーゼを標的とした新規抗ウイルス薬探索のための蛍光発色によるアッセイ法の開発.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed January 20, 2021. http://hdl.handle.net/10069/36837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ulanday, Gianne Eduard Limbo. “Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against Dengue 2 viral protease : デング2型ウイルスプロテアーゼを標的とした新規抗ウイルス薬探索のための蛍光発色によるアッセイ法の開発.” 2016. Web. 20 Jan 2021.

Vancouver:

Ulanday GEL. Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against Dengue 2 viral protease : デング2型ウイルスプロテアーゼを標的とした新規抗ウイルス薬探索のための蛍光発色によるアッセイ法の開発. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10069/36837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ulanday GEL. Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against Dengue 2 viral protease : デング2型ウイルスプロテアーゼを標的とした新規抗ウイルス薬探索のための蛍光発色によるアッセイ法の開発. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/36837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. EDUARDO REZENDE HONDA. Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO.

Degree: 2005, Fundação Universidade Federal de Rondônia

URL: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=19 ; http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=20

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A proteína não estrutural NS1, não tem função atribuída na fisiopatologia da infecção, embora haja evidencias de que participe das etapas iniciais de replicação viral.… (more)

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A proteína não estrutural NS1, não tem função atribuída na fisiopatologia da infecção, embora haja evidencias de que participe das etapas iniciais de replicação viral. Sabe-se, por outro lado que ela é secretada no plasma de indivíduos infectados nas fases precoces da infecção viral e que o sistema imunológico produz anticorpos precocemente contra essa proteína. Por isso, a proteína NS1 se coloca como candidata a produção de antígeno recombinante a ser utilizada em testes de diagnóstico precoce da infecção. No presente trabalho, como etapa preparatória da produção de proteínas recombinante, procurou-se analisar o grau de conservação da proteína em diferentes isolados virais. Amplificamos e seqüenciamos assim o gene NS1 de 3 amostras de vírus dengue sorotipo 1, isolados de pacientes em Porto Velho, durante o surto ocorrido entre fevereiro e abril de 2001. A partir das três amostras seqüenciadas foi definida uma seqüência consenso representativa dessa região do genoma de vírus locais.A seqüência consenso foi, em seguida comparada as seqüências da mesma região do genoma de viris isolados na região sul, nordeste e sudeste do país, publicadas recentemente por Santos e cols, Virus Reseach, 2002) além de cepas de outras regiões do mundo, registradas no GENBANK. Comparando nossas seqüências com as de outras regiões do Brasil, observamos que a taxa de mutação afetando seqüências de aminoácidos entre as proteínas alinhadas, foi de 5,1%;3,63% dessas mutações provocam substituições por aminoácidos com alto grau de similaridade, 0,56% produzem substituições sem nenhum grau de similaridade. Na análise utilizando nossas seqüências derivadas de outras partes do mundo, observou-se taxas de mutação de 4,8%, sendo 3,4% mutações para aminoácidos com elevado grau de similaridade,1,4% de mutações sem nenhum grau de similaridade.

The non structural protein NS1 of the dengue virus still has no clearly defined function in the fhysiopathology of the infection, although there are evidences that it may participate in the early stages of viral replication. The NS1 protein is secreted into the plasma of infected individuals during the early phase of infection and the humoral immune system process antibodies against it. Therefore, the NS1 protein may be useful as marker of early infection. In the present work, as preparatory stage of the production of recombinants NS1 proteins, we analyzed the degree of conservation of the NS1 gene different isolates. We amplified, cloned an sequenced the NS1 gene from 3 samples of Dengue serotype 1, isolated of patients in Porto Velho, during an outbreak between February and April of 2001. From the three sequences a consensus sequence of the locally circulating virus was defined. This consensus sequence was compared to the sequences of viruses from southern, southeastern and northeastern Brazil (Nunes et al, Virology reseach,2002), and also to sequence from other regions of the word. When comparing our sequences with the sequences of other areas of Brazil, we observed 5,1% of aminoacid substitutions. 3,63% of…

Advisors/Committee Members: Luiz Hildebrando Pereira da Silva.

Subjects/Keywords: Gene; Virus; Gene; Dengue; Protein; BIOLOGIA GERAL; Proteína; Vírus; Dengue

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APA (6^th Edition):

HONDA, E. R. (2005). Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO. (Thesis). Fundação Universidade Federal de Rondônia. Retrieved from http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=19 ; http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=20

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

HONDA, EDUARDO REZENDE. “Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO.” 2005. Thesis, Fundação Universidade Federal de Rondônia. Accessed January 20, 2021. http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=19 ; http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=20.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

HONDA, EDUARDO REZENDE. “Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO.” 2005. Web. 20 Jan 2021.

Vancouver:

HONDA ER. Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO. [Internet] [Thesis]. Fundação Universidade Federal de Rondônia; 2005. [cited 2021 Jan 20]. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=19 ; http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=20.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

HONDA ER. Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO. [Thesis]. Fundação Universidade Federal de Rondônia; 2005. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=19 ; http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=20

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. DEUSILENE SOUZA VIEIRA. IDENTIFICAÇÃO E CARACTERIZAÇÃO DO VÍRUS DO DENGUE EM ÁREAS URBANAS E PERI-URBANAS DE PORTO VELHO-RO.

Degree: 2005, Fundação Universidade Federal de Rondônia

URL: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=4

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The dengue is the more important arboviruses which afect the humans in terms of mortality and morbidity, characterized as febrile Illness acute autolimited, which the… (more)

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The dengue is the more important arboviruses which afect the humans in terms of mortality and morbidity, characterized as febrile Illness acute autolimited, which the etiologic agent related to Flavivirus genus. The Rondonia State is located in Amazon Region, north of Brazil, being a considered State triple favorable the propagation of the dengue virus, therefore is connected by road to others parts of country; for being border with another country and still to exist population migrations The dengue virus identification and molecular characterization are important for serotyping determination circulating in the region, the source of virus and the correlation of virulence and epidemiology. This dissertation has as objective of standardize of techniques for identification and molecular characterization of dengue virus in urban and peri-urbans areas from Porto Velho. Collected blood samples of patients with clinical suspects of dengue fever between the months of January/2001 and December/2003. Isolate the virus using the C6/36 cells and confirmed by immufluorescence assay. The RNA viral was extracted for two methods: the TRIzol and QUIAamp RNA viral mini kit . After, submitted the extracted RNA to RT-PCR with two different pairs of primers. The first pairs, AD3 and AD4 identify the dengue virus and the serotyping characterization was made with restriction enzymes Kpn I and Hind III, for dengue virus serotype 1 and 2 respectively. Again, for identification of dengue virus used the primers D1 and D2, following of a Hemi-nested-PCR for serotyping characterization of dengue virus type 1, 2, 3 and 4. One hundred and fifteen blood samples were collected of patients. Seventy were positive in immunofluorescence assay. Forty sample were extracted RNA viral by TRIzol an 30 samples by QIAamp RNA mini kit . Sixty were characterized as dengue virus type 1 for restriction enzymes method. Teen sample were characterized by Hemi-nested-PCR as dengue virus type 3 This study showed which the dengue virus type 1 and 3 circulate in Porto Velho; was possible standardized the technique for RNA viral extraction for RT-PCR directly of serum samples and establishment of techniques for epidemiological surveillance. The sensibility of RNA extraction directly of serum samples was relation with the viremia.

A dengue é a mais importante arbovirose que afeta o homem em termos de morbidade e mortalidade caracteriza-se como sendo uma doença febril aguda autolimitante, cujo agente etiológico pertence ao gênero Flavivirus. O Estado de Rondônia encontra-se localizado na região norte do país dentro a da Amazônia Ocidental sendo considerado um Estado triplamente favorável à propagação do vírus dengue, pois é. ligado por rodovia as demais regiões do país;. por ser fronteira e por existir ainda migrações populacionais. A identificação e caracterização molecular do vírus da dengue são importantes para determinar o sorotipo circulante em uma região; a origem do vírus e a tentativa de se estabelecer uma correlação de virulência das amostras e sua importância…

Advisors/Committee Members: Luiz Hildebrando Pereira da Silva.

Subjects/Keywords: urban; Dengue; Virus; Dengue; BIOLOGIA GERAL; vírus; urbanas

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APA (6^th Edition):

VIEIRA, D. S. (2005). IDENTIFICAÇÃO E CARACTERIZAÇÃO DO VÍRUS DO DENGUE EM ÁREAS URBANAS E PERI-URBANAS DE PORTO VELHO-RO. (Thesis). Fundação Universidade Federal de Rondônia. Retrieved from http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

VIEIRA, DEUSILENE SOUZA. “IDENTIFICAÇÃO E CARACTERIZAÇÃO DO VÍRUS DO DENGUE EM ÁREAS URBANAS E PERI-URBANAS DE PORTO VELHO-RO.” 2005. Thesis, Fundação Universidade Federal de Rondônia. Accessed January 20, 2021. http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

VIEIRA, DEUSILENE SOUZA. “IDENTIFICAÇÃO E CARACTERIZAÇÃO DO VÍRUS DO DENGUE EM ÁREAS URBANAS E PERI-URBANAS DE PORTO VELHO-RO.” 2005. Web. 20 Jan 2021.

Vancouver:

VIEIRA DS. IDENTIFICAÇÃO E CARACTERIZAÇÃO DO VÍRUS DO DENGUE EM ÁREAS URBANAS E PERI-URBANAS DE PORTO VELHO-RO. [Internet] [Thesis]. Fundação Universidade Federal de Rondônia; 2005. [cited 2021 Jan 20]. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

VIEIRA DS. IDENTIFICAÇÃO E CARACTERIZAÇÃO DO VÍRUS DO DENGUE EM ÁREAS URBANAS E PERI-URBANAS DE PORTO VELHO-RO. [Thesis]. Fundação Universidade Federal de Rondônia; 2005. Available from: http://www.bdtd.unir.br/tde_busca/arquivo.php?codArquivo=4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Riverside

9. Wasik, Daniel. Development of Carbon Nanotube-Based Biosensors to Detect Dengue Virus.

Degree: Environmental Toxicology, 2017, University of California – Riverside

URL: http://www.escholarship.org/uc/item/3tg1t3x4

► Since 1970, human activities have permitted the spread of Dengue virus (DENV) and the primary mosquito vectors Aedes spp. to virtually every continent. Infection rates… (more)

▼ Since 1970, human activities have permitted the spread of Dengue virus (DENV) and the primary mosquito vectors Aedes spp. to virtually every continent. Infection rates have increased more than 30-fold and it has become the most prevalent arboviral disease in the world. Every year, 3.6 billion people are at risk of infection and there are 390 million new infections, mostly among children. With no vaccine or specific treatment, early detection plays a significant role in decreasing fatality rates. Dengue infection has no pathognomonic clinical features, thus diagnostic tools are essential for diagnosis.In addition to a human transmission cycle, a variety of forest-dwelling non-human primates are hosts for DENV in a sylvatic cycle. Unfortunately, sylvatic cross-over events occur regularly and have resulted in disease outbreaks within humans. Vector surveillance plays a critical role in dengue detection and outbreak prevention. Current laboratory methods for detection and diagnosis of DENV require highly trained personnel and costly equipment that are impractical for regular surveillance and diagnostic use. Thus, new technologies to facilitate and enhance diagnostic and surveillance capabilities within each transmission cycle are urgently needed. This research describes the development of two novel biosensors using single-walled carbon nanotube transducers functionalized for the detection of whole DENV or DENV Non-Structural Protein 1 (NS1). Heparin, an analog of the heparan sulfate proteoglycans that are receptors for DENV, was used as a bioreceptor for detection of whole DENV virions within viral culture. This permits detection of DENV virions from a variety of viral culture-compatible samples; such as fluid or tissue samples from monkeys, vector mosquitos, and humans. Anti-dengue NS1 monoclonal antibodies were used to detect DENV NS1, a clinically accepted biomarker for DENV infection. This biosensor will allow early detection and diagnosis of the disease in Aedes mosquitos and human saliva. Both biosensors were selective and sensitive for their target analyte in a 10-μL sample over the clinically relevant concentration range with detection occurring in only 10-20 min. Each was constructed to be a portable, rapid, and inexpensive diagnostic tool suitable for use by minimally-trained personnel in the field, laboratory, or point-of-care location.

Subjects/Keywords: Nanotechnology; Virology; Biosensor; Carbon nanotubes; Chemiresistor; Dengue NS1; Dengue virus; Immunosensor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wasik, D. (2017). Development of Carbon Nanotube-Based Biosensors to Detect Dengue Virus. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/3tg1t3x4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wasik, Daniel. “Development of Carbon Nanotube-Based Biosensors to Detect Dengue Virus.” 2017. Thesis, University of California – Riverside. Accessed January 20, 2021. http://www.escholarship.org/uc/item/3tg1t3x4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wasik, Daniel. “Development of Carbon Nanotube-Based Biosensors to Detect Dengue Virus.” 2017. Web. 20 Jan 2021.

Vancouver:

Wasik D. Development of Carbon Nanotube-Based Biosensors to Detect Dengue Virus. [Internet] [Thesis]. University of California – Riverside; 2017. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/3tg1t3x4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wasik D. Development of Carbon Nanotube-Based Biosensors to Detect Dengue Virus. [Thesis]. University of California – Riverside; 2017. Available from: http://www.escholarship.org/uc/item/3tg1t3x4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

10. Chiu, Wei-Wei. Translation of dengue virus RNA : influence of the untranslated regions on 5´-cap dependent translation and ribosome scanning.

Degree: PhD, Microbiology, 2006, Oregon State University

URL: http://hdl.handle.net/1957/3053

► In this thesis, I studied the translation of dengue virus RNA using a luciferase reporter gene system in Vero cells. The dengue reporter mRNA construct,… (more)

▼ In this thesis, I studied the translation of dengue virus RNA using a luciferase reporter gene system in Vero cells. The dengue reporter mRNA construct, which harbors 5´-terminal viral nts and 3´-terminal nts, could be translated efficiently compared to an alpha globin reporter construct. The 5´-cap structure and 3´-untranslated region (UTR) of dengue virus RNA were found to contribute to strong enhancement of translation. The 5´-cap and 3´-UTR act synergistically to enhance translation like the 5´-cap and 3´-polyA tail of a cellular mRNA. Deletion analysis of each individual structural element in the dengue 3´-UTR indicated a cumulative effect in enhancing dengue translation. The cyclization sequences, which are present in the 5´ and 3´ portion of the genome to facilitate the end-to-end interactio, were tested for their importance in translation. The finding that a mutation of the sequence in the 3´-UTR, but not in the 5´, exhibited a deteriorating effect, indicates that cyclization of the genome via the sequences is not required for efficient dengue translation. The importance of a 5´-cap in dengue RNA translation was supported by using an inhibitor of cap-dependent translation, LY294002, whose presence significantly inhibited dengue reporter RNA translation in vivo. Dengue viral replication was also found to be sensitive to the presence of inhibitor, indicating that dengue viral translation does not take advantage of inhibition of cap-dependent cellular translation, unlike mRNA with an internal ribosomal entry site. Consistent with our finding of 5´-cap dependency of dengue RNA translation, introduction of a stable stem loop structure in front of the dengue reporter construct significantly inhibited translation, strongly suggesting that dengue RNA does not harbor an internal ribosome entry site. To examine whether ribosomes scan through the 5´-UTR of dengue RNA, various upstream AUGs (uAUG) were introduced in the 5´-UTR of a dengue construct with a green fluorescent reporter protein. uAUG containing reporter constructs showed reduced translation from the authentic AUG in vivo and in rabbit reticulocyte lysate, suggesting that dengue translation is utilizing the conventional scanning mode of ribosome for AUG recognition. All together, dengue virus utilizes the cap-dependent scanning mode for translation initiation. Advisors/Committee Members: Dreher, Theo W (advisor), Rohrmann, Geroge (committee member).

Subjects/Keywords: Dengue virus; Dengue viruses

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chiu, W. (2006). Translation of dengue virus RNA : influence of the untranslated regions on 5´-cap dependent translation and ribosome scanning. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/3053

Chicago Manual of Style (16^th Edition):

Chiu, Wei-Wei. “Translation of dengue virus RNA : influence of the untranslated regions on 5´-cap dependent translation and ribosome scanning.” 2006. Doctoral Dissertation, Oregon State University. Accessed January 20, 2021. http://hdl.handle.net/1957/3053.

MLA Handbook (7^th Edition):

Chiu, Wei-Wei. “Translation of dengue virus RNA : influence of the untranslated regions on 5´-cap dependent translation and ribosome scanning.” 2006. Web. 20 Jan 2021.

Vancouver:

Chiu W. Translation of dengue virus RNA : influence of the untranslated regions on 5´-cap dependent translation and ribosome scanning. [Internet] [Doctoral dissertation]. Oregon State University; 2006. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1957/3053.

Council of Science Editors:

Chiu W. Translation of dengue virus RNA : influence of the untranslated regions on 5´-cap dependent translation and ribosome scanning. [Doctoral Dissertation]. Oregon State University; 2006. Available from: http://hdl.handle.net/1957/3053

Penn State University

11. Rabaa, Maia Anita. The molecular evolution and phylogeography of dengue viruses in Asia.

Degree: 2012, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/16341

► The global burden of dengue and its impact on the health and economics of tropical countries is vast and continues to grow. Outbreaks of severe… (more)

▼ The global burden of dengue and its impact on the health and economics of tropical countries is vast and continues to grow. Outbreaks of severe dengue disease are increasingly common in regions where dengue was thought to be rare only a decade ago. Our knowledge of the epidemiological and evolutionary processes by which dengue viruses (DENV) invade new populations and become established as persistent viral lineages has been limited by a lack of data relating virus to host and vector populations, as well as the immune landscapes in which they spread over time. Virus lineages enter new populations regularly, but become established only occasionally and are generally detected long after establishment has occurred. The introduction of novel viral lineages has been shown to perturb the immune landscape in endemic environments, often causing massive epidemics and long-term changes in clinical manifestations within the host population. The use of large sets of spatially- and temporally- related DENV genome and envelope gene (E) sequences to investigate these lineage establishment events allows us to trace the timing and routes of viral movement between host populations, including the evolutionary dynamics of the viral lineages themselves. Determining whether trends in viral introduction and establishment can be distinguished within DENV phylogenies, and what these trends may be, is integral to understanding and controlling the spread of disease at local, regional, and global scales. In this thesis, I explore the spatio-temporal dynamics of DENV dispersal to identify the routes by which dengue invades new populations, characterize trends in viral migration, and identify the factors that affect the speed and scope of DENV migration at various scales and in heterogeneous transmission environments. In hyperendemic settings, I determined that DENV diversity is maintained in densely populated urban areas, frequently spreading through urban and suburban populations and into rural areas, and following a predictable pattern of human movement that suggests a limited role for the vector in the rapid dispersal of an emerging lineage across the population. Once arriving in rural areas, transmission is remarkably spatially and temporally focal. Where climate conditions are suitable, these populations may show persistence of viral populations over multiple seasons, but the likelihood of lineage fade-out here is increased as a result of lower population densities and seasonal bottlenecks in DENV populations. Hyperendemic urban areas further seed more distant populations at rates that appear to be dependent upon transmission intensities in the donor population and the connectedness of the human host populations, while the establishment of viral populations may show a greater dependence upon the immune landscape and the climate into which the virus is introduced. This is also true at the largest spatial and temporal scales, where I identify three geographic regions in tropical Asia that act as sources of global DENV diversity and suggest… Advisors/Committee Members: Edward C Holmes, Dissertation Advisor/Co-Advisor, Andrew Fraser Read, Committee Chair/Co-Chair, Isabella Cattadori, Committee Member, Timothy Reluga, Committee Member.

Subjects/Keywords: dengue; molecular evolution; phylogeography; virus evolution

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rabaa, M. A. (2012). The molecular evolution and phylogeography of dengue viruses in Asia. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/16341

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rabaa, Maia Anita. “The molecular evolution and phylogeography of dengue viruses in Asia.” 2012. Thesis, Penn State University. Accessed January 20, 2021. https://submit-etda.libraries.psu.edu/catalog/16341.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rabaa, Maia Anita. “The molecular evolution and phylogeography of dengue viruses in Asia.” 2012. Web. 20 Jan 2021.

Vancouver:

Rabaa MA. The molecular evolution and phylogeography of dengue viruses in Asia. [Internet] [Thesis]. Penn State University; 2012. [cited 2021 Jan 20]. Available from: https://submit-etda.libraries.psu.edu/catalog/16341.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rabaa MA. The molecular evolution and phylogeography of dengue viruses in Asia. [Thesis]. Penn State University; 2012. Available from: https://submit-etda.libraries.psu.edu/catalog/16341

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Figueiredo, Mario Luis Garcia de. Identificação de flavivirus infectando culicídeos de 1999 a 2007 no Brasil.

Degree: PhD, Microbiologia, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19052010-153321/ ;

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Introdução: Arbovírus são vírus transmitidos por artrópodos, pertencendo, principalmente, aos gêneros Flavivirus (Flaviviridae), Alphavirus (Togaviridae) e Orthobunyavirus (Bunyavirus). Os Flavivirus, em sua maioria, são associados… (more)

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Introdução: Arbovírus são vírus transmitidos por artrópodos, pertencendo, principalmente, aos gêneros Flavivirus (Flaviviridae), Alphavirus (Togaviridae) e Orthobunyavirus (Bunyavirus). Os Flavivirus, em sua maioria, são associados a zoonoses, causando doenças humanas febrís, febres hemorrágicas e encefalites. Inclusive, causam epidemias que são sério problema de saúde pública. Este estudo mostra uma pesquisa de Flavivirus em culicídeos, de diferentes regiões do país, utilizando uma técnica para identificação viral por RT-PCR com primers Flavivirusespecíficos e uma Multiplex-nested-PCR com primers espécie-específicos. Métodos: Culicídeos foram capturados, quantificados, identificados, agrupados em lotes por espécie e congelados. No laboratório, os animais foram macerados e tiveram o RNA extraído. Estes extratos foram submetidos a RT-PCR gênero-específica e à Multiplex-nested-PCR, para detecção e identificação dos vírus a nível de espécie. Resultado: De 3317 culicídios adultos e 571 larvas coletados em 4 diferentes regiões do Brasil, Sul, Sudeste, Norte e Nordeste, fez-se 246 lotes de mosquitos e desses foi possível obter amplicon sugestivo de Flavivirus em 16 (6,5%). Em 3 lotes contendo larvas de Aedes albopictus obteve-se amplicon sugestivo de vírus do dengue tipo 3. Também, em 13 lotes contendo Haemagogus leucocelaenus, Aedes aegypti e Aedes albopictus foi possível obter amplicons sugestivos de vírus do dengue tipos 1 e 2. Dos amplicons obtidos, 4 tiveram nucleotídios seqüenciados o que permitiu confirmar a presença dos vírus do dengue tipo 3 e Cacipacoré. Conclusão: O trabalho permitiu concluir que: a metodologia de RT-PCR para Flavivirus seguida de Multiplex-nested-PCR espécie-específica foi adequada para detecção e identificação destes vírus em culicídios; amplificaram-se genomas de Flavivirus em 6,5% dos lotes de culicídios estudados; vírus do dengue tipo 1 e tipo 2 foram encontrados infectando Aedes aegypti de Santos em 1999, Manaus em 2005-2006 e Foz do Iguaçu; vírus do dengue tipos 2 e 3 foram encontrados em Aedes albopictus de Santos em 1999 e Manaus em 2005-2006, sugerindo que este mosquito participe na transmissão de dengue; vírus do dengue tipo 3 foi encontrado em larvas de Aedes albopictus mostrando transmissão vertical do vírus; vírus do dengue tipo 1 foi encontrado infectando Haemagogus sp. sugerindo existência de ciclo silvático deste vírus; Aedes aegypti do Amazonas estavam infectados com o vírus Cacipacoré.

Introduction: Arbovirus are rodent-borne viruses mostly from Flavivirus (Flaviviridae), Alphavirus (Togaviridae) e Orthobunyavirus (Bunyavirus) genus. Flavivirus, are commonly zoonotic and can cause febrile illness, haemorrhagic fever and encephalitis. Flavivirus outbreaks occur in Brazil and are a major public health problem. We show here a research looking for Flavivirus infections in Culicidae by a RT-PCR using Flavivirus-especific primers and a Multiplex-nested-PCR using specie-specific primers for virus identification. Methods: Culicidae were captured, quantified, identified, pooled…

Advisors/Committee Members: Durigon, Edison Luiz.

Subjects/Keywords: Arbovirus; Arbovírus; Cacipacoré virus; Culicídeos; Culicídeos; Dengue virus; Flavivirus; Flavivirus; Vírus Cacipacoré; Vírus da dengue

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Figueiredo, M. L. G. d. (2010). Identificação de flavivirus infectando culicídeos de 1999 a 2007 no Brasil. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19052010-153321/ ;

Chicago Manual of Style (16^th Edition):

Figueiredo, Mario Luis Garcia de. “Identificação de flavivirus infectando culicídeos de 1999 a 2007 no Brasil.” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 20, 2021. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19052010-153321/ ;.

MLA Handbook (7^th Edition):

Figueiredo, Mario Luis Garcia de. “Identificação de flavivirus infectando culicídeos de 1999 a 2007 no Brasil.” 2010. Web. 20 Jan 2021.

Vancouver:

Figueiredo MLGd. Identificação de flavivirus infectando culicídeos de 1999 a 2007 no Brasil. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 20]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19052010-153321/ ;.

Council of Science Editors:

Figueiredo MLGd. Identificação de flavivirus infectando culicídeos de 1999 a 2007 no Brasil. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19052010-153321/ ;

13. Celina de Oliveira Poersch. Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue.

Degree: 2007, Fundação Oswaldo Cruz

URL: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=131

► A dengue é hoje a mais importante arbovirose que afeta o homem, constituindo um sério problema de saúde pública no mundo, especialmente em países tropicais… (more)

▼ A dengue é hoje a mais importante arbovirose que afeta o homem, constituindo um sério problema de saúde pública no mundo, especialmente em países tropicais onde as condições do meio ambiente favorecem a proliferação dos seus vetores, os mosquitos do gênero Aedes. Atualmente, quase metade da população mundial vive em áreas de risco para a doença e, apesar de sua extensão e gravidade, vacinas ou tratamentos específicos ainda não estão disponíveis. Tendo conhecimento da urgência na melhoria da vigilância epidemiológica da dengue e das deficiências do diagnóstico laboratorial dessa e de outras doenças no país, realizou-se esse trabalho cujo principal objetivo foi o desenvolvimento de métodos diagnósticos baseados em técnicas moleculares de última geração para a detecção e sorotipagem do vírus da dengue. As metodologias escolhidas foram as de PCR em tempo real e de microarranjo líquido, as quais representam os mais recentes avanços na área de diagnóstico molecular. Diferentes conjuntos de oligonucleotídeos sorotipo- e grupo-específicos foram avaliados quanto a capacidade de amplificar e identificar os sorotipos DENV1, DENV2 e DENV3 pelos dois métodos. Dessa forma, desenvolveu-se, baseado na técnica de PCR em tempo real, três ensaios uniplex sorotipo-específicos para dengue e um teste grupo-específico capaz de amplificar todos os sorotipos do vírus. Para o ensaio baseado na técnica de microarranjo líquido os oligonucleotídeos, após testados individualmente, foram combinados em diferentes formatos multiplex, tanto na reação de amplificação (PCR) como na de hibridação. Diferentes protocolos de hibridação foram também avaliados. Quando testada contra curvas padrões obtidas a partir da diluição de quantidades conhecidas dos três sorotipos do vírus, a PCR em tempo real mostrou-se, para todos os ensaios, capaz de amplificar e detectar mesmo a menor quantidade de vírus avaliada, equivalente a 53,57 FFU/ml. Já a sensibilidade do teste de microarranjo líquido multiplex foi de 3.428,48 FFU/ml para DENV1 e 53,57 FFU/ml para DENV2 e DENV3, com os oligonucleotídeos sorotipo-específicos e 214,28 FFU/ml para DENV3 e 53,57 FFU/ml para DENV1 e DENV2, com os oligonucleotídeos grupo-específicos. A especificidade dos dois métodos foi de 100%. A fim de avaliar a capacidade desses testes em diagnosticar e sorotipar o vírus da dengue em amostras clínicas, foram usados 50 soros positivos para IgM anti-dengue, 49 negativos por sorologia e 49 soros de pacientes cuja positividade para dengue foi confirmada por isolamento viral. A concordância entre os dois métodos foi de 92,5% quando comparados os resultados obtidos com os oligonucleotídeos grupo-específicos e de 90,5% para os ensaios sorotipo-específicos. Tomando o grupo positivo por isolamento viral como referência, o método de microarranjo líquido falhou em detectar três amostras que foram consideradas positivas por PCR em tempo real e detectou uma amostra que não foi detectada na PCR. Embora o método baseado em microarranjo líquido tenha apresentado menor sensibilidade do que a PCR em tempo… Advisors/Committee Members: Marco Aurélio Krieger.

Subjects/Keywords: BIOLOGIA MOLECULAR; Dengue; Vírus da Dengue; Aedes; Reação em Cadeia da Polimerase; Humanos; Animais; Dengue; Dengue Virus; Aedes; Polymerase Chain Reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Poersch, C. d. O. (2007). Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue. (Thesis). Fundação Oswaldo Cruz. Retrieved from http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=131

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Poersch, Celina de Oliveira. “Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue.” 2007. Thesis, Fundação Oswaldo Cruz. Accessed January 20, 2021. http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=131.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Poersch, Celina de Oliveira. “Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue.” 2007. Web. 20 Jan 2021.

Vancouver:

Poersch CdO. Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue. [Internet] [Thesis]. Fundação Oswaldo Cruz; 2007. [cited 2021 Jan 20]. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=131.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Poersch CdO. Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue. [Thesis]. Fundação Oswaldo Cruz; 2007. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=131

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Purdue University

14. Chang, Jinsam. Role of NS1 in virus replication using Dengue virus and West Nile virus chimeras.

Degree: MS, Biological Science, 2015, Purdue University

URL: https://docs.lib.purdue.edu/open_access_theses/1172

► The flavivirus non-structural protein 1 (NS1) is translocated into the endoplasmic reticulum (ER), glycosylated, and secreted from the infected cell. Among its various functions, a… (more)

Subjects/Keywords: chimera; Dengue Virus; Non structural protein 1; West Nile Virus

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APA (6^th Edition):

Chang, J. (2015). Role of NS1 in virus replication using Dengue virus and West Nile virus chimeras. (Thesis). Purdue University. Retrieved from https://docs.lib.purdue.edu/open_access_theses/1172

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Jinsam. “Role of NS1 in virus replication using Dengue virus and West Nile virus chimeras.” 2015. Thesis, Purdue University. Accessed January 20, 2021. https://docs.lib.purdue.edu/open_access_theses/1172.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Jinsam. “Role of NS1 in virus replication using Dengue virus and West Nile virus chimeras.” 2015. Web. 20 Jan 2021.

Vancouver:

Chang J. Role of NS1 in virus replication using Dengue virus and West Nile virus chimeras. [Internet] [Thesis]. Purdue University; 2015. [cited 2021 Jan 20]. Available from: https://docs.lib.purdue.edu/open_access_theses/1172.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang J. Role of NS1 in virus replication using Dengue virus and West Nile virus chimeras. [Thesis]. Purdue University; 2015. Available from: https://docs.lib.purdue.edu/open_access_theses/1172

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

15. Thu, Hlaing Myat. Virus diversity and the emergence of Dengue.

Degree: 2004, Queensland University of Technology

URL: https://eprints.qut.edu.au/16081/

► The aims of this study were to investigate the role of the diversity of dengue virus populations in changing patterns of virus transmission and disease.… (more)

▼ The aims of this study were to investigate the role of the diversity of dengue virus populations in changing patterns of virus transmission and disease. Prior to the commencement of this study, dengue 2 virus (DENV-2) had been associated most frequently with severe disease, so the study commenced with this serotype. Because it was not possible to quantitate diversity in the entire 11 kb of the viral genome, the study focussed on the envelope (E) gene, because the E protein is the major protein on the surface of the virion and thus might be under strong selective pressure from the host immune system and from the requirement to engage specific receptors on host cells. This study was the first direct quantification of the diversity of dengue virus populations in individual hosts. The nucleotide sequences of more than 70 per cent of the E genes in each virus population differed from the consensus nucleotide sequence for the population. In the course of quantitating genetic diversity in DENV-2 virus populations in patients and in mosquitoes, recombinant DENV-2 and both parental virus populations were detected in a single mosquito. This was the first such report. In 2001, just after the commencement of this study, Myanmar had the largest outbreak of dengue on record. Unlike previous outbreaks, 95 per cent of dengue viruses isolated from patients were of a single serotype, DENV-1. Despite the large number of cases of dengue, the proportion of patients with severe dengue was low. In the light of these observations, the direction of this study changed to focus on DENV-1. Phylogenetic analysis of the E genes of DENV-1 collected before and after the 2001 dengue outbreak suggested that some time before 1998, an early lineage of DENV-1 had become extinct and had been replaced by two new lineages. There was no evidence that these changes were due to selection or to recombination within the E protein genes of the old clade of viruses and the newly introduced viruses. A more detailed analysis was undertaken, of the entire genome of 11 human DENV-1 isolates and of 4 from mosquitoes recovered in Yangon between 1971 and 2002, to determine whether the extinction of the pre-1998 lineage of DENV-1 (clade A) and the appearance of the two new lineages (clades B and C) could have been due to selective pressures acting on genes other than E. Evidence of only weak selection was found in the NS5 gene (at amino acids 127,135 and 669) but the resultant amino acid changes did not distinguish all recent viruses from viruses belonging to the extinct clade. The phylogenetic relationships between individual genes from these viruses and between the open reading frames were similar. No evidence was found of recombination that might have given rise to two new clades of virus with enhanced fitness. Collectively, these data suggested that the extinction of clade A viruses and their replacement by the two new clades, between 1998 and 2000 was a stochastic event in an inter-epidemic period when rates of virus transmission were low. This was the…

Subjects/Keywords: Virus; Virus Diversity; Dengue

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APA (6^th Edition):

Thu, H. M. (2004). Virus diversity and the emergence of Dengue. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/16081/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thu, Hlaing Myat. “Virus diversity and the emergence of Dengue.” 2004. Thesis, Queensland University of Technology. Accessed January 20, 2021. https://eprints.qut.edu.au/16081/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thu, Hlaing Myat. “Virus diversity and the emergence of Dengue.” 2004. Web. 20 Jan 2021.

Vancouver:

Thu HM. Virus diversity and the emergence of Dengue. [Internet] [Thesis]. Queensland University of Technology; 2004. [cited 2021 Jan 20]. Available from: https://eprints.qut.edu.au/16081/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thu HM. Virus diversity and the emergence of Dengue. [Thesis]. Queensland University of Technology; 2004. Available from: https://eprints.qut.edu.au/16081/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

16. Eichen, Eva. Antibody dependent enhancement: a model for understanding congenital Zika syndrome.

Degree: MS, Physician Assistant Program, 2018, Boston University

URL: http://hdl.handle.net/2144/32971

► This literature review will discuss Zika virus and the salient research on antibody dependent enhancement and how this mechanism may lead to congenital defects. Specific… (more)

Subjects/Keywords: Virology; Brazil; Antibody dependent enhancement; Dengue virus; Zika virus

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APA (6^th Edition):

Eichen, E. (2018). Antibody dependent enhancement: a model for understanding congenital Zika syndrome. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/32971

Chicago Manual of Style (16^th Edition):

Eichen, Eva. “Antibody dependent enhancement: a model for understanding congenital Zika syndrome.” 2018. Masters Thesis, Boston University. Accessed January 20, 2021. http://hdl.handle.net/2144/32971.

MLA Handbook (7^th Edition):

Eichen, Eva. “Antibody dependent enhancement: a model for understanding congenital Zika syndrome.” 2018. Web. 20 Jan 2021.

Vancouver:

Eichen E. Antibody dependent enhancement: a model for understanding congenital Zika syndrome. [Internet] [Masters thesis]. Boston University; 2018. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2144/32971.

Council of Science Editors:

Eichen E. Antibody dependent enhancement: a model for understanding congenital Zika syndrome. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/32971

17. Garcia Luna, Selene M. Mexican mosquitoes: overcoming barriers for dengue and Zika virus infection.

Degree: PhD, Microbiology, Immunology, and Pathology, 2017, Colorado State University

URL: http://hdl.handle.net/10217/185645

► The mosquito transmitted arboviruses cause an important burden of disease worldwide. In Latin America dengue disease is endemic with more than 1 million dengue fever… (more)

▼ The mosquito transmitted arboviruses cause an important burden of disease worldwide. In Latin America dengue disease is endemic with more than 1 million dengue fever cases reported yearly. In addition to dengue, chikungunya and Zika viruses have been also circulating since their introduction in 2014 and 2015 respectively. For a mosquito-borne infection to occur susceptible humans, the mosquito vector and the virus should coincide. This dissertation was focused in the mosquito vector and its ability to acquire, maintain and then transmit the virus, termed vector competence. The vector competence was a fundamental measure for the research chapters in which we studied different aspects on the interactions between Aedes aegypti and Aedes albopictus mosquitoes and Dengue-2 and Zika viruses. This dissertation includes three research chapters which were based on the following specific aims. Specific aim 1: Determine the patterns of gene flow and vector competence for DENV-2 of Aedes aegypti from around the Mexican Neovolcanic Axis. It was previously reported that the intersection of the Neovolcanic axis (NVA) with the Gulf of Mexico coast in the state of Veracruz acts as a discrete barrier to gene flow among Ae. aegypti populations north and south of the NVA. These collections also differed in their vector competence (VC) for Dengue virus serotype 2 (DENV-2). Therefore, the goal of the present study was to determine if the same patterns remained 8 years later in collections from 2012. For which haplotype variation for the mitochondrial ND4 and the nuclear genes Dicer-2 and Argonaute-2 was analyzed for north and south of the NVA mosquito populations. Also, the VC of those populations for DENV-2 was determined (Chapter 2). Specific aim 2: Profile the microRNA response of Aedes aegypti midguts to DENV-2 exposure and DENV-2 infection. The microRNA pathway has been found to modulate important physiological mechanisms in mosquito vectors. Therefore in the context of DENV infection, miRNA modulation may provide information about key genes that are important for infection. Differential expression patterns of miRNAs from mosquito midguts upon infection have been unexplored. Therefore, we explored on the involvement of the miRNA pathway in persistently DENV-2 infected mosquitoes, for which DENV-2 virus was detected at 14 days post-infection (dpi). Two comparisons were included in the study. In the first group, DENV-2 infected midguts that produced a disseminated infection (did not have a midgut escape barrier) were contrasted with those that were given a non-infectious blood meal. Also, we included a comparison group from a subset of mosquitoes from the same cohort that were exposed to DENV-2 regardless of their midgut infection status contrasted to unexposed mosquitoes. Analysis of miRNA regulation in mosquitoes may help us to understand more about the intricate interactions between the virus and the vector host (Chapter 3). Specific aim 3: Assess the variation in competence for Zika virus transmission by Aedes aegypti and Aedes… Advisors/Committee Members: Black, William C., IV (advisor), Ebel, Gregory D. (committee member), Perera, Rushika (committee member), Hess, Ann M. (committee member).

Subjects/Keywords: Dengue virus; vector competence; Mexico; Aedes; Zika virus

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Garcia Luna, S. M. (2017). Mexican mosquitoes: overcoming barriers for dengue and Zika virus infection. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/185645

Chicago Manual of Style (16^th Edition):

Garcia Luna, Selene M. “Mexican mosquitoes: overcoming barriers for dengue and Zika virus infection.” 2017. Doctoral Dissertation, Colorado State University. Accessed January 20, 2021. http://hdl.handle.net/10217/185645.

MLA Handbook (7^th Edition):

Garcia Luna, Selene M. “Mexican mosquitoes: overcoming barriers for dengue and Zika virus infection.” 2017. Web. 20 Jan 2021.

Vancouver:

Garcia Luna SM. Mexican mosquitoes: overcoming barriers for dengue and Zika virus infection. [Internet] [Doctoral dissertation]. Colorado State University; 2017. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10217/185645.

Council of Science Editors:

Garcia Luna SM. Mexican mosquitoes: overcoming barriers for dengue and Zika virus infection. [Doctoral Dissertation]. Colorado State University; 2017. Available from: http://hdl.handle.net/10217/185645

Arizona State University

18. Abdelgalel, Rowida Abdelgalel. Vaccination Strategy To Protect Against Flavivirus Infection Based On Recombinant Measles Vaccine.

Degree: Microbiology, 2016, Arizona State University

URL: http://repository.asu.edu/items/40803

► Despite the approval of a Dengue virus (DV) vaccine in five endemic countries, dengue prevention would benefit from an immunization strategy highly immunogenic in young… (more)

▼ Despite the approval of a Dengue virus (DV) vaccine in five endemic countries, dengue prevention would benefit from an immunization strategy highly immunogenic in young infants and not curtailed by viral interference. Problematically, infants younger than 9 year of age, whom are particularly prone to Dengue severe infection and death, cannot be immunized using current approved DV vaccine. The most important issues documented so far are the lack of efficiency and enhancement of the disease in young seronegative recipients, as well as uneven protection against the four DV serotypes. Based on data from clinical trials that showed enhanced performance of dengue vaccines when the host has previous anti-flaviviral immunity, I proposed here an attractive solution to complement the current vaccine: a recombinant measles vaccine vectoring dengue protective antigens to be administered to young infants. I hypothesized that recombinant measles virus expressing Dengue 2 and 4 antigens would successfully induce neutralizing responses against DV2 and 4 and the vaccine cocktail of this recombinant measles can prime anti-flaviviral neutralizing immunity. For this dissertation, I generated and performed preclinical immune assessment for four novel Measles-Dengue (MV-DV) vaccine candidates. I generated four MVs expressing the pre membrane (prM) and full length or truncated (90%) forms of the major envelope (E) from DV2 and DV4. Two virus, MVvac2-DV2(prME)N and MVvac2-DV4(prME), expressed high levels of membrane associated full-length E, while the other two viruses, MVvac2-DV2(prMEsol)N and MVvac2-DV4(prMEsol)N, expressed and secreted truncated, soluble E protein to its extracellular environment. The last two vectored vaccines proved superior anti-dengue neutralizing responses comparing to its corresponding full length vectors. Remarkably, when MVvac2-DV2/4(prMEsol)N recombinant vaccines were combined, the vaccine cocktail was able to prime cross-neutralizing responses against DV 1 and the relatively distant 17D yellow fever virus attenuated strain. Thus, I identify a promising DV vaccination strategy, MVvac2-DV2/4(prMEsol)N, which can prime broad neutralizing immune responses by using only two of the four available DV serotypes. The current MV immunization scheme can be advantageus to prime broad anti-flaviviral neutralizing immunity status, which will be majorly boosted by subsequent chimeric Dengue vaccine approaches.

Subjects/Keywords: Microbiology; Virology; Molecular biology; Cross neutralization; Dengue vaccine; Dengue virus; Flavivirus; Measles virus; Yellow fever virus

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APA (6^th Edition):

Abdelgalel, R. A. (2016). Vaccination Strategy To Protect Against Flavivirus Infection Based On Recombinant Measles Vaccine. (Doctoral Dissertation). Arizona State University. Retrieved from http://repository.asu.edu/items/40803

Chicago Manual of Style (16^th Edition):

Abdelgalel, Rowida Abdelgalel. “Vaccination Strategy To Protect Against Flavivirus Infection Based On Recombinant Measles Vaccine.” 2016. Doctoral Dissertation, Arizona State University. Accessed January 20, 2021. http://repository.asu.edu/items/40803.

MLA Handbook (7^th Edition):

Abdelgalel, Rowida Abdelgalel. “Vaccination Strategy To Protect Against Flavivirus Infection Based On Recombinant Measles Vaccine.” 2016. Web. 20 Jan 2021.

Vancouver:

Abdelgalel RA. Vaccination Strategy To Protect Against Flavivirus Infection Based On Recombinant Measles Vaccine. [Internet] [Doctoral dissertation]. Arizona State University; 2016. [cited 2021 Jan 20]. Available from: http://repository.asu.edu/items/40803.

Council of Science Editors:

Abdelgalel RA. Vaccination Strategy To Protect Against Flavivirus Infection Based On Recombinant Measles Vaccine. [Doctoral Dissertation]. Arizona State University; 2016. Available from: http://repository.asu.edu/items/40803

University of Connecticut

19. Chen, Shubing. Development of Multiplex Real Time Reverse Transcriptase Polymerase Chain Reaction Detection among Zika Virus, Yellow Fever Virus and Dengue Virus Type 4.

Degree: MS, Pathobiology, 2018, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/1259

► Zika virus, Dengue virus and Yellow Fever virus are three important Flaviviruses that are epidemic and endemic in Central Africa, Middle and South America,… (more)

Subjects/Keywords: multiplex real time RT-PCR; Zika virus; Yellow Fever Virus; Dengue Virus

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APA (6^th Edition):

Chen, S. (2018). Development of Multiplex Real Time Reverse Transcriptase Polymerase Chain Reaction Detection among Zika Virus, Yellow Fever Virus and Dengue Virus Type 4. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/1259

Chicago Manual of Style (16^th Edition):

Chen, Shubing. “Development of Multiplex Real Time Reverse Transcriptase Polymerase Chain Reaction Detection among Zika Virus, Yellow Fever Virus and Dengue Virus Type 4.” 2018. Masters Thesis, University of Connecticut. Accessed January 20, 2021. https://opencommons.uconn.edu/gs_theses/1259.

MLA Handbook (7^th Edition):

Chen, Shubing. “Development of Multiplex Real Time Reverse Transcriptase Polymerase Chain Reaction Detection among Zika Virus, Yellow Fever Virus and Dengue Virus Type 4.” 2018. Web. 20 Jan 2021.

Vancouver:

Chen S. Development of Multiplex Real Time Reverse Transcriptase Polymerase Chain Reaction Detection among Zika Virus, Yellow Fever Virus and Dengue Virus Type 4. [Internet] [Masters thesis]. University of Connecticut; 2018. [cited 2021 Jan 20]. Available from: https://opencommons.uconn.edu/gs_theses/1259.

Council of Science Editors:

Chen S. Development of Multiplex Real Time Reverse Transcriptase Polymerase Chain Reaction Detection among Zika Virus, Yellow Fever Virus and Dengue Virus Type 4. [Masters Thesis]. University of Connecticut; 2018. Available from: https://opencommons.uconn.edu/gs_theses/1259

Universidade Federal do Maranhão

20. JULIANA MARIA TRINDADE BEZERRA. POPULATION DENSITY OF Aedes aegypti (DIPTERA: CULICIDAE) AND RATE OF DENGUE VIRUS INFECTION IN SÃO LUIS, MARANHÃO.

Degree: 2010, Universidade Federal do Maranhão

URL: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=527

►

This study aimed to obtain Aedes aegypti density population and to identify the presence of dengue virus in adults vector collected at different periods of… (more)

▼

This study aimed to obtain Aedes aegypti density population and to identify the presence of dengue virus in adults vector collected at different periods of the year in São Luís, Maranhão. Mosquitoes were collected in three periods: dry (November and December 2008), rainy (March and April 2009) and intermediate (July and August 2009). Were visited 320 properties in eight neighborhoods previously drawn from two districts: Coréia de Baixo, Lira, Goiabal and João Paulo (Distrito Centro) and Itapiracó, Residential Canudos, Conjunto Cohatrac I and Vila Luisão (Distrito COHAB). We used mechanical vacuum on the battery to catch A. aegypti adults. After counting, identification and storage, the specimens were sent to the Instituto Evandro Chagas, in Ananindeua, State of Pará, to carry out the molecular and viral analysis for virus isolation and Reverse Transcription followed by Polymerase Chain Reaction (RTPCR). Also obtained climatological data for the period of study. We collected 563 mosquitoes A. aegypti, with predominance of adults in the rainy season, but not have significant difference in the amount of specimens of A. aegypti per period. There was also no positive and negative correlation between the factors and the number of A. aegypti adults. About the neighborhoods, João Paulo and Goiabal were obtained statistically significant densities. Specimens of A. aegypti were divided into pools, considering the neighborhoods of collection, with 13 formed in the dry season, 23 in the rainy season and 15 in the intermediate period. Viral isolation and RT-PCR of samples were not positive for the attested dengue virus. These results show that A. aegypti density increased during the rainy season and the João Paulo and Goiabal neighborhoods had the largest number of specimens, showing that in São Luís, the climatic factors may to influence the vector seasonal fluctuation.

Este estudo objetivou obter a densidade populacional de Aedes aegypti e identificar a presença de vírus dengue em formas aladas do vetor coletadas em diferentes períodos do ano no município de São Luís, Maranhão. Foram coletados mosquitos em três períodos: seco (novembro e dezembro de 2008), chuvoso (março e abril de 2009), e intermediário (julho e agosto de 2009). Foram visitados 320 imóveis em oito bairros previamente sorteados de dois distritos: Coréia de Baixo, Lira, Goiabal e João Paulo (Distrito Centro); e Itapiracó, Residencial Canudos, Conjunto Cohatrac I e Vila Luisão (Distrito Cohab). Utilizou-se aspirador mecânico ligado a bateria para captura dos alados. Após contagem, identificação e armazenamento, os espécimes foram encaminhados ao Instituto Evandro Chagas, em Ananindeua, Estado do Pará, para realização das análises viral e molecular por isolamento viral e Transcrição Reversa seguida pela Reação em Cadeia da Polimerase (RT-PCR). Também foram obtidos os dados climatológicos referentes ao período de estudo. Foram coletados 563 mosquitos A. aegypti, com predominância de alados no período chuvoso, não havendo diferença significativa na quantidade de…

Advisors/Committee Members: Valéria Cristina Soares Pinheiro, Emygdia Rosa do Rego Barros Pires Leal Mesquita.

Subjects/Keywords: Vírus; A. aegypti; A. aegypti; SAUDE MATERNO-INFANTIL; Virus; Variáveis climáticas; Climatic variables; Dengue; Dengue

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APA (6^th Edition):

BEZERRA, J. M. T. (2010). POPULATION DENSITY OF Aedes aegypti (DIPTERA: CULICIDAE) AND RATE OF DENGUE VIRUS INFECTION IN SÃO LUIS, MARANHÃO. (Thesis). Universidade Federal do Maranhão. Retrieved from http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=527

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

BEZERRA, JULIANA MARIA TRINDADE. “POPULATION DENSITY OF Aedes aegypti (DIPTERA: CULICIDAE) AND RATE OF DENGUE VIRUS INFECTION IN SÃO LUIS, MARANHÃO.” 2010. Thesis, Universidade Federal do Maranhão. Accessed January 20, 2021. http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=527.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

BEZERRA, JULIANA MARIA TRINDADE. “POPULATION DENSITY OF Aedes aegypti (DIPTERA: CULICIDAE) AND RATE OF DENGUE VIRUS INFECTION IN SÃO LUIS, MARANHÃO.” 2010. Web. 20 Jan 2021.

Vancouver:

BEZERRA JMT. POPULATION DENSITY OF Aedes aegypti (DIPTERA: CULICIDAE) AND RATE OF DENGUE VIRUS INFECTION IN SÃO LUIS, MARANHÃO. [Internet] [Thesis]. Universidade Federal do Maranhão; 2010. [cited 2021 Jan 20]. Available from: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=527.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

BEZERRA JMT. POPULATION DENSITY OF Aedes aegypti (DIPTERA: CULICIDAE) AND RATE OF DENGUE VIRUS INFECTION IN SÃO LUIS, MARANHÃO. [Thesis]. Universidade Federal do Maranhão; 2010. Available from: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=527

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Tran, Tuan Anh. Screening against the dengue virus polymerase : Criblage contre la polymérase du virus de la dengue.

Degree: Docteur es, Biologie, 2016, Aix Marseille Université

URL: http://www.theses.fr/2016AIXM4006

►

La dengue, une des maladies les plus largement émergents actuellement, avec 390 millions d'infections chaque année (OMS), est causée par le virus de la dengue… (more)

▼

La dengue, une des maladies les plus largement émergents actuellement, avec 390 millions d'infections chaque année (OMS), est causée par le virus de la dengue contre lequel il n’existe pas de traitements. La protéine NS5 a un rôle important dans le cycle de réplication. Cette protéine se compose d'une méthionine S-transférase d’adénosyl en N-terminal et une ARN polymérase dépendante de l'ARN (RdRp) en C-terminal. Cette NS5 RdRp peut catalyser non seulement la synthèse du brin négatif de l'ARN, utilisé comme matrice pour synthétiser l'ARN brin plus-supplémentaire, mais aussi pour la synthèse d'un ARN complémentaire à partir d'une matrice court e d'ARN sans amorce (de novo). Dans ce travail de thèse, nous présentons la production et le test de l'activité de la protéine NS5, ainsi que du domaine polymérase RdRp pour les quatre sérotypes du virus de la dengue en développant un nouveau test enzymatique, en utilisant comme un réactif fluorescent. L'utilisation de ce réactif fluorescent a également contribué à la détermination des conditions optimisées pour développer un essai de criblage de l'activité polymérase pour identifier des inhibiteurs contre le virus de la dengue. En outre, quatre flavonoïdes, Hinokiflavone, apigénine, la quercétine et Amentoflavone ont montré des valeurs d’IC50 équivalentes contre toutes les constructions NS5 et les domaines polymérase des quatre sérotypes.

Dengue fever, one of the most widely emerging diseases nowadays with 390 million infections each year (WHO), is caused by Dengue virus in which no official antiviral reagent or vaccine is available. The NS5 protein has an important role in the replication cycle. This protein consists of a S-adenosyl methionine transferase at N-terminal and a RNA dependent RNA polymerase (RdRp) at C-terminal. This NS5 RdRp can catalyse for not only synthesis of minus-strand RNA to be used as the template to synthesize additional plus-strand RNA but also synthesizing a complement RNA from a short RNA template without primer (de novo). In this research we present the production and activity test for NS5 protein and N-terminal extended sequence 266-900 from NS5 RdRp of all first four serotypes of Dengue virus and a construct of sequence 273-900 using a new enzymatic assay, using Picogreen as fluorescent reagent. Using this fluorescent reagent also helped determining the optimised conditions to develop a screening assay for inhibitors against dengue polymerase activity. In addition, four flavonoids, Hinokiflavone, Apigenin, Quercetin and Amentoflavone showed approximate IC50 values when testing on all NS5 and polymerase protein constructs of all four serotypes.

Advisors/Committee Members: Guillemot, Jean-Claude (thesis director).

Subjects/Keywords: Virus de la dengue; Ns5; Polymérase; Réplication; Inhibiteur; Picogreen; Dengue virus; Ns5; Polymerase; Replication; Inhibitor; Picogreen; 570

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APA (6^th Edition):

Tran, T. A. (2016). Screening against the dengue virus polymerase : Criblage contre la polymérase du virus de la dengue. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2016AIXM4006

Chicago Manual of Style (16^th Edition):

Tran, Tuan Anh. “Screening against the dengue virus polymerase : Criblage contre la polymérase du virus de la dengue.” 2016. Doctoral Dissertation, Aix Marseille Université. Accessed January 20, 2021. http://www.theses.fr/2016AIXM4006.

MLA Handbook (7^th Edition):

Tran, Tuan Anh. “Screening against the dengue virus polymerase : Criblage contre la polymérase du virus de la dengue.” 2016. Web. 20 Jan 2021.

Vancouver:

Tran TA. Screening against the dengue virus polymerase : Criblage contre la polymérase du virus de la dengue. [Internet] [Doctoral dissertation]. Aix Marseille Université 2016. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2016AIXM4006.

Council of Science Editors:

Tran TA. Screening against the dengue virus polymerase : Criblage contre la polymérase du virus de la dengue. [Doctoral Dissertation]. Aix Marseille Université 2016. Available from: http://www.theses.fr/2016AIXM4006

22. Milhas, Sabine. Développement d'outils pour l'étude des interactions protéine-protéine : Development of tools for the study of protein-protein interaction.

Degree: Docteur es, Biologie, 2016, Aix Marseille Université

URL: http://www.theses.fr/2016AIXM4020

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Au cours de ma thèse je me suis intéressée aux interactions protéine-protéine (PPI’s). Les PPI’s jouent un rôle majeur dans une grande diversité de processus… (more)

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Au cours de ma thèse je me suis intéressée aux interactions protéine-protéine (PPI’s). Les PPI’s jouent un rôle majeur dans une grande diversité de processus cellulaires et sont maintenant considérées comme une cible majeure dans le but de développer de nouveaux médicaments. Cependant, cibler ce type d’interactions requiert le développement de chimiothèques dédiées, permettant d’accélérer la découverte de molécules « touches ». Pour surmonter ce problème, une chimiothèque orientée PPI (2P2I3D) a été conçu au laboratoire. Dans un premier temps, j’ai donc évalué cette chimiothèque sur différents complexes possédant des interfaces variées. Les résultats obtenus ont révélé des taux de touches supérieurs à ceux obtenus avec des chimiothèques non orientées, de 0,2 à 1,6% contre 0,01 à 0,1%, respectivement. Cette étude a permis d’établir une preuve de concept de la faisabilité de créer une chimiothèque orientée PPI, permettant ainsi une accélération de la découverte de composés biologiquement actifs.Dans un deuxième temps, je me suis intéressée à l’interaction entre deux protéines majeures du virus de la dengue : les protéines NS3 et NS5. J’ai tout d’abord identifié et caractérisé un nouveau site d’interaction, ce qui m’a permis de mettre en évidence que cette interaction avait pour conséquence d’augmenter l’activité enzymatique du domaine hélicase. J’ai par la suite recherché et identifié des petites molécules chimiques capable d’inhiber cette interaction. Les différentes caractérisations effectuées ont permis de mettre en évidence un effet antiviral. Ces inhibiteurs constituent un excellent point de départ afin d’étudier plus en détail le rôle biologique de ce complexe.

In my thesis I became interested in protein-protein interactions (PPI's). PPI's play a major role in a variety of cellular processes and are now considered a major target in order to develop new drugs. However, targeting such interactions requires the development of dedicated libraries, to accelerate the discovery of “hits”molecules .To overcome this issue, a focused chemical library PPI (2P2I3D) was designed in the laboratory.At first, I evaluated this chemical library on different complexes with diverse interfaces. The results showed higher hit rate to those obtained with non-oriented libraries, from 0.2 to 1.6% against 0.01 to 0.1%, respectively. This study has established a proof of concept of the feasibility of creating a focused chemical library PPI, thus accelerating the discovery of biologically active compounds.Secondly, I am interested in the interaction between two major proteins of dengue virus: the NS3 and NS5 proteins. I initially identified and characterized a novel interaction site, which allowed me to demonstrate that this interaction had the effect of increasing the enzymatic activity of the helicase domain. I searched and identified small molecules able to inhibit this interaction. The different characterizations helped to highlight an antiviral effect. These inhibitors are an excellent starting point to further explore the biological…

Advisors/Committee Members: Guillemot, Jean-Claude (thesis director), Morelli, Xavier (thesis director).

Subjects/Keywords: Interaction protéine-Protéine; Inhibiteurs; Virus de la dengue; Ns3; Ns5; Protein protein interaction; Inhibitors; Dengue virus; Ns3; Ns5; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Milhas, S. (2016). Développement d'outils pour l'étude des interactions protéine-protéine : Development of tools for the study of protein-protein interaction. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2016AIXM4020

Chicago Manual of Style (16^th Edition):

Milhas, Sabine. “Développement d'outils pour l'étude des interactions protéine-protéine : Development of tools for the study of protein-protein interaction.” 2016. Doctoral Dissertation, Aix Marseille Université. Accessed January 20, 2021. http://www.theses.fr/2016AIXM4020.

MLA Handbook (7^th Edition):

Milhas, Sabine. “Développement d'outils pour l'étude des interactions protéine-protéine : Development of tools for the study of protein-protein interaction.” 2016. Web. 20 Jan 2021.

Vancouver:

Milhas S. Développement d'outils pour l'étude des interactions protéine-protéine : Development of tools for the study of protein-protein interaction. [Internet] [Doctoral dissertation]. Aix Marseille Université 2016. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2016AIXM4020.

Council of Science Editors:

Milhas S. Développement d'outils pour l'étude des interactions protéine-protéine : Development of tools for the study of protein-protein interaction. [Doctoral Dissertation]. Aix Marseille Université 2016. Available from: http://www.theses.fr/2016AIXM4020

23. Mantel, Nathalie. Contribution à la caractérisation moléculaire et cellulaire des chimères YF-17D / Dengue dans le cadre du développement préclinique du vaccin Dengvaxia® : Contribution to the molecular and cellular characterization of YF-17D/Dengue chimera as part of the preclinical development of Dengvaxia® vaccine.

Degree: Docteur es, Virologie, 2018, Lyon

URL: http://www.theses.fr/2018LYSE1034

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Sanofi Pasteur travaille depuis plus de 20 ans au développement d’un vaccin contre la Dengue, maladie virale pouvant présenter des formes sévères. Ce vaccin, dénommé… (more)

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Sanofi Pasteur travaille depuis plus de 20 ans au développement d’un vaccin contre la Dengue, maladie virale pouvant présenter des formes sévères. Ce vaccin, dénommé Dengue CYD est composé de quatre virus recombinants basés sur la souche vaccinale contre la Fièvre Jaune dans laquelle les gènes codant les protéines prM et E ont été remplacés par ceux des différents sérotypes de virus Dengue.Dans les études décrites ici, nous avons démontré la stabilité génétique des souches vaccinales au cours des étapes de production, et nous avons mis au point un système de qRT-PCR pour quantifier le génome viral afin de caractériser les lots et suivre les virémies post-vaccinales.De plus, différentes études précliniques menées chez le macaque ont permis :1) d’évaluer l’immunogénicité du vaccin après immunisation par différentes formulations vaccinales et selon différents schémas visant à réduire les interférences entre les sérotypes. L’administration de vaccins bivalents complémentaires à des sites anatomiques différents ou de façon séquentielle, l’établissement d’une pré-immunité hétérologue, une moindre dose relative du sérotype vaccinal dominant ou l’administration d’un rappel à un an ont ainsi permis de mettre en évidence des pistes d’amélioration du schéma vaccinal.2) d’évaluer la biodistribution et l’excrétion du vaccin afin de confirmer son innocuité.3) de tester la neutralisation d’un panel de souches virales par des sérums des singes vaccinés pour montrer que les anticorps induits par le vaccin peuvent neutraliser des souches Dengue circulantes d’origines géographiques et de génotypes variés.Enfin, l’absence de risque de dissémination du virus dans l’environnement via les tiques, arthropodes vecteurs d’autres Flavivirus, a été confirmée.Ces études ont permis d’apporter des éléments démontrant l’intérêt du vaccin Dengue CYD pour lancer des études cliniques et compléter les dossiers réglementaires visant à l’enregistrement du vaccin Dengvaxia®

Sanofi Pasteur has been working for more than 20 years to develop a vaccine against Dengue, viral disease that can cause life-threatening forms. The CYD Dengue vaccine is a tetravalent recombinant virus based on Yellow fever vaccine strain in which genes encoding prM and E proteins have been replaced by the corresponding genes from the different Dengue virus serotypes.In the studies described here, we demonstrated the genetic stability of the vaccine strains during the manufacturing steps and we set-up a qRT-PCR system to quantify viral genome in order to characterize batches and to follow post-vaccination viremia.In addition, different pre-clinical studies were conducted in macaques in order:1) To evaluate the vaccine immunogenicity after immunizations according to different schedules and formulations aiming at decreasing interferences between serotypes. Different parameters were shown to improve vaccine immunogenicity, such as administration of complementary bivalent vaccines at separate anatomical sites or sequentially, establishment of a heterologous pre-immunity, adaptation of…

Advisors/Committee Members: Guy, Bruno (thesis director).

Subjects/Keywords: Dengue; Vaccin; Virus; Caractérisation moléculaire; Etudes précliniques; Primates Non-Humain; Dengue; Vaccine; Virus; Molecular Characterization; Preclinical studies; Non-Human Primates; 579.2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mantel, N. (2018). Contribution à la caractérisation moléculaire et cellulaire des chimères YF-17D / Dengue dans le cadre du développement préclinique du vaccin Dengvaxia® : Contribution to the molecular and cellular characterization of YF-17D/Dengue chimera as part of the preclinical development of Dengvaxia® vaccine. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2018LYSE1034

Chicago Manual of Style (16^th Edition):

Mantel, Nathalie. “Contribution à la caractérisation moléculaire et cellulaire des chimères YF-17D / Dengue dans le cadre du développement préclinique du vaccin Dengvaxia® : Contribution to the molecular and cellular characterization of YF-17D/Dengue chimera as part of the preclinical development of Dengvaxia® vaccine.” 2018. Doctoral Dissertation, Lyon. Accessed January 20, 2021. http://www.theses.fr/2018LYSE1034.

MLA Handbook (7^th Edition):

Mantel, Nathalie. “Contribution à la caractérisation moléculaire et cellulaire des chimères YF-17D / Dengue dans le cadre du développement préclinique du vaccin Dengvaxia® : Contribution to the molecular and cellular characterization of YF-17D/Dengue chimera as part of the preclinical development of Dengvaxia® vaccine.” 2018. Web. 20 Jan 2021.

Vancouver:

Mantel N. Contribution à la caractérisation moléculaire et cellulaire des chimères YF-17D / Dengue dans le cadre du développement préclinique du vaccin Dengvaxia® : Contribution to the molecular and cellular characterization of YF-17D/Dengue chimera as part of the preclinical development of Dengvaxia® vaccine. [Internet] [Doctoral dissertation]. Lyon; 2018. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2018LYSE1034.

Council of Science Editors:

Mantel N. Contribution à la caractérisation moléculaire et cellulaire des chimères YF-17D / Dengue dans le cadre du développement préclinique du vaccin Dengvaxia® : Contribution to the molecular and cellular characterization of YF-17D/Dengue chimera as part of the preclinical development of Dengvaxia® vaccine. [Doctoral Dissertation]. Lyon; 2018. Available from: http://www.theses.fr/2018LYSE1034

24. Devignot, Stéphanie. Le Syndrome de choc de dengue, approches clinique et in vitro : Dengue shock syndrome, clinical and in vitro investigations.

Degree: Docteur es, Pathologie humaine, 2010, Aix-Marseille 2

URL: http://www.theses.fr/2010AIX20672

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Le syndrome de choc de dengue (DSS) est une complication potentiellement mortelle dela dengue, première arbovirose humaine et problème majeur de santé publiquemondial. Il survient… (more)

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Le syndrome de choc de dengue (DSS) est une complication potentiellement mortelle dela dengue, première arbovirose humaine et problème majeur de santé publiquemondial. Il survient chez une fraction des patients, et résulte d’une fuite plasmatiquemassive, non prédictible, dont la physiopathologie est mal connue. Le décryptage de laréponse de l’hôte est donc essentiel pour améliorer le pronostic et le traitement despatients. Ce travail de thèse a abordé les mécanismes de la fuite plasmatique du DSS dedeux façons : versant immunitaire et versant endothélial. D’une part, nous avons comparéen ex vivo les profils transcriptionnels sanguins de patients présentant différentes formescliniques de dengue, afin d’identifier des mécanismes contribuant à la survenue du DSS.Cette étude a révélé l’activation chez les patients en DSS, de signatures proinflammatoiresà l’interface entre immunité innée et métabolisme lipidique, représentantde nouveaux bio-marqueurs potentiels du DSS. D’autre part, les études in vitro desinteractions entre un virus de dengue et deux lignées de cellules endothélialesmicrovasculaires humaines (CEM), a révélé des différences d’intensité de réponseantivirale, ainsi que des différences dans l’expression de protéines impliquées dans laperméabilité, selon l’origine des territoires endothéliaux. Ces résultats suggèrent que levirus contribue directement au dysfonctionnement endothélial, au côté de mécanismesindirects médiés par des facteurs de l’hôte. Les deux types d’approches mises en oeuvreont ainsi établi de nouvelles données sur la physiopathologie du DSS, qui pourraient àterme trouver des applications dans la prise en charge des malades.

Dengue Shock Syndrome (DSS) is a life-threatening form of dengue infection, which is thefirst arboviral disease worldwide and a major public health problem. This severecomplication happens in a fraction of patients, and is the consequence of anunpredictable massive plasma leakage. The pathophysiology underlying DSS is stillunknown. Deciphering the host response to dengue infection is essential to improve boththe prognosis and the therapeutic management of dengue patients. This thesis workintended to study the mechanisms involved in DSS’ plasma leakage at both immunity (exvivo study) and endothelium (in vitro study) levels. First, in a ex vivo study, we comparedwhole blood cells’ transcriptional profiles of patients suffering from different clinicalpresentations of dengue disease, in order to identify mechanisms contributing to DSSoutcome. This study revealed the activation of pro-inflammatory signatures at theinterface of innate immunity and lipid metabolism, in DSS patients. Those signatures maybe new bio-markers of DSS. Second, in vitro studies of the consequences of a directinteraction between a dengue virus and human microvascular endothelial cells (MEC),revealed differences in antiviral response intensities and in the expression of proteinsinvolved in the endothelial permeability, depending on the endothelial origin of theMEC. Those results suggest that the virus…

Advisors/Committee Members: Tolou, Hugues (thesis director), Couissinier-Paris, Patricia (thesis director).

Subjects/Keywords: Syndrome de choc de dengue; Physiopathologie; Endothélium; Inflammation; Relation hôte-virus; Dengue Shock Syndrome; Pathophysiology; Endothélium; Inflammation; Host-virus relationship

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Devignot, S. (2010). Le Syndrome de choc de dengue, approches clinique et in vitro : Dengue shock syndrome, clinical and in vitro investigations. (Doctoral Dissertation). Aix-Marseille 2. Retrieved from http://www.theses.fr/2010AIX20672

Chicago Manual of Style (16^th Edition):

Devignot, Stéphanie. “Le Syndrome de choc de dengue, approches clinique et in vitro : Dengue shock syndrome, clinical and in vitro investigations.” 2010. Doctoral Dissertation, Aix-Marseille 2. Accessed January 20, 2021. http://www.theses.fr/2010AIX20672.

MLA Handbook (7^th Edition):

Devignot, Stéphanie. “Le Syndrome de choc de dengue, approches clinique et in vitro : Dengue shock syndrome, clinical and in vitro investigations.” 2010. Web. 20 Jan 2021.

Vancouver:

Devignot S. Le Syndrome de choc de dengue, approches clinique et in vitro : Dengue shock syndrome, clinical and in vitro investigations. [Internet] [Doctoral dissertation]. Aix-Marseille 2; 2010. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2010AIX20672.

Council of Science Editors:

Devignot S. Le Syndrome de choc de dengue, approches clinique et in vitro : Dengue shock syndrome, clinical and in vitro investigations. [Doctoral Dissertation]. Aix-Marseille 2; 2010. Available from: http://www.theses.fr/2010AIX20672

25. Potisopon, Supanee. Insights into the RNA polymerase activity of the dengue virus NS5 : Study of protein-protein complexes involving the low molecular weight chaperone HSP27 : Implications in prostate cancer.

Degree: Docteur es, Pathologie humaine. Maladies infectieuses, 2014, Aix Marseille Université

URL: http://www.theses.fr/2014AIXM5019

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Le virus de la dengue cause une maladie de type grippal qui peut dans certains cas évoluer vers des fièvreshémorragiques mortelles. Mon projet de thèse… (more)

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Le virus de la dengue cause une maladie de type grippal qui peut dans certains cas évoluer vers des fièvreshémorragiques mortelles. Mon projet de thèse porte sur la réplication de ce virus. Je focalise sur la compréhension du mécanisme d'action de la protéine NS5 de ce virus. La protéine contient 2 domaines : 1) domaine méthyltransférase, essentiel pour la traduction des protéines virales, 2) domaine polymérase, synthétisant le génome ARN du virus. Premièrement, nous avons démontré que la polymérase joue un rôle principal dans la conservation de l'extrémité 3' et 5' du génome et de l'anti-génome. Puis, j'ai caractérisé l'influence du domaine méthyltransférase sur l'activité polymérase de la protéine NS5. J'ai développé un système d'études mécanistiques en utilisant des techniques biochimiques de cinétique pré-stationnaire pour la protéine NS5, et obtenu des paramètres cinétiques et thermodynamiques de cette protéine envers ses substrats. Avec ce même système, j'ai pu tester des activités de la polymérase NS5 avec des ARN coiffés et triphosphates de différente longueur, mimant les séquences à l'extrémité 5' du génome du virus de la dengue. L'activité polymérase de NS5 est influencée par la présence de la coiffe de l'ARN, ce qui m'a permis de proposer une distance physique correspondant à environ 13 nucléotides entre les sites actifs domaines méthyltransférase et polymérase. Mes travaux ouvrent la voie à la détermination de la structure 3D de NS5 avec ses ARN et des nucléotides 5'-triphosphate.Elucider son mécanisme d'action, c'est être capable d'inhiber son action et donc de pouvoir proposer des molécules capables d'arrêter la prolifération virale lors d'une infection.

Dengue virus causes dengue fever, which may evolve towards life-threatening hemorrhagic fever. My research projectfocuses on dengue replication, and more precisely on the mechanism of NS5 at the molecular/atomic level. NS5 is a bifunctionalenzyme containing two domains: 1) a methyltransferase domain essential for translation of viral proteins, 2) apolymerase domain synthesizing the viral RNA genome. First, we demonstrated the main role of the polymerase in theconservation of 5' and 3' ends of dengue genome and anti-genome RNAs. Next, I showed the influence of themethyltransferase domain on the activity of the polymerase domain. I also developed a system allowing mechanistic studiesusing pre-steady state kinetics to characterize NS5 in depth. I have made use of this system to determine the catalyticparameters of NS5 towards its substrates. Using the same pre-steady state system, I was able to test the polymerase activityof NS5 with capped and uncapped 5'-triphosphate RNAs of different lengths corresponding to the 5'-end of the dengue RNAgenome. The polymerase activity of NS5 is significantly affected by the presence of the 5'-cap, which allowed me to designan experimental set-up pointing to a minimal physical distance of around 13 nucleotides between the methyltransferase andpolymerase active sites. My work will be useful to characterize the…

Advisors/Committee Members: Canard, Bruno (thesis director), Selisko, Barbara (thesis director).

Subjects/Keywords: Mot clés : virus de la dengue; Ns5; Polymérase; Méthyltransférase; Synthèse de l'ARN; Réplication; Dengue virus; Ns5; Polymerase; Methyltransferase; RNA synthesis; Replication

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Potisopon, S. (2014). Insights into the RNA polymerase activity of the dengue virus NS5 : Study of protein-protein complexes involving the low molecular weight chaperone HSP27 : Implications in prostate cancer. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2014AIXM5019

Chicago Manual of Style (16^th Edition):

Potisopon, Supanee. “Insights into the RNA polymerase activity of the dengue virus NS5 : Study of protein-protein complexes involving the low molecular weight chaperone HSP27 : Implications in prostate cancer.” 2014. Doctoral Dissertation, Aix Marseille Université. Accessed January 20, 2021. http://www.theses.fr/2014AIXM5019.

MLA Handbook (7^th Edition):

Potisopon, Supanee. “Insights into the RNA polymerase activity of the dengue virus NS5 : Study of protein-protein complexes involving the low molecular weight chaperone HSP27 : Implications in prostate cancer.” 2014. Web. 20 Jan 2021.

Vancouver:

Potisopon S. Insights into the RNA polymerase activity of the dengue virus NS5 : Study of protein-protein complexes involving the low molecular weight chaperone HSP27 : Implications in prostate cancer. [Internet] [Doctoral dissertation]. Aix Marseille Université 2014. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2014AIXM5019.

Council of Science Editors:

Potisopon S. Insights into the RNA polymerase activity of the dengue virus NS5 : Study of protein-protein complexes involving the low molecular weight chaperone HSP27 : Implications in prostate cancer. [Doctoral Dissertation]. Aix Marseille Université 2014. Available from: http://www.theses.fr/2014AIXM5019

26. Vial, Thomas. Le virus de la dengue détourne le métabolisme des phospholipides du moustique pour sa réplication : Dengue virus diverts the mosquito phospholipid metabolism for replication.

Degree: Docteur es, Microbiologie, 2020, Université Toulouse III – Paul Sabatier

URL: http://www.theses.fr/2020TOU30036

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Plus de la moitié de la population mondiale est exposée au risque d'infection par le virus de la dengue (DENV) en raison de la distribution… (more)

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Plus de la moitié de la population mondiale est exposée au risque d'infection par le virus de la dengue (DENV) en raison de la distribution mondiale de ses moustiques vecteurs. Il n'existe ni vaccin ni traitement efficace. La seule stratégie disponible repose sur les insecticides, contre lesquels les moustiques développent une résistance. Les virus utilisent le métabolome de l'hôte pour la réplication et la dissémination. C'est particulièrement vrai pour les virus enveloppés comme le DENV qui dépend des membranes lipidiques de l'hôte pour compléter son cycle de vie. Pour atteindre un environnement métabolique optimal, les virus perturbent le métabolome de l'hôte. La compréhension de ces altérations chez les moustiques vecteurs pourrait révéler de nouvelles stratégies pour bloquer la transmission du DENV. Ici, nous avons caractérisé comment le DENV détourne le lipidome du moustique Aedes aegypti. Pour décrire les changements métaboliques tout au long du cycle du DENV chez le moustique, nous avons débeloppé une méthode de chromatographie liquide et de spectrométrie de masse à haute résolution (LC-HRMS) à différents stades de l'infection chez le vecteur. Nous avons révélé une reconfiguration majeure des phospholipides tout au long du cycle du DENV chez le moustique, dans les cellules, l'intestin moyen et le moustique entier. Pour déchiffrer la façon dont le virus reconfigure les phospholipides, nous avons caractérisé phylogénétiquement les isoformes de l'enzyme acylglycerol-phosphate acyltransférase (AGPAT) et identifié celles qui catalysent une étape limitante dans la biogenèse des phospholipides. Nous avons constaté que l'infection par le DENV diminuait l'expression de AGPAT1, dont la déplétion renforce l'infection en maintenant des concentrations élevées d'aminophospholipides (aminoPL), en particulier la phosphatidylcholine (PC) et la phosphatidyléthanolamine (PE), pendant le cycle du DENV chez le moustique. En démontrant que la sous-régulation de AGPAT1, causé par le virus, fournit un environnement proviral, nous révèlons le premier facteur métabolique hôte chez les moustiques et soulignent le rôle des aminophospholipides dans le cycle cellulaire viral. Nous avons ensuite cherché à confirmer que le virus influence la biosynthèse des aminoPL et déterminer à quel stade du cycle viral la reconfiguration des aminoPL est nécessaire. La biosynthèse de novo de PC et de PE est connue sous le nom de voie de Kennedy, où un diacylglycérol (DAG) incorpore soit un groupe choline, soit un groupe éthanolamine. [...]

More than half of the world population is at risk of dengue virus (DENV) infection because of the global distribution of its mosquito vectors. There is neither effective vaccine nor therapeutics. The only available strategy relies on insecticides, against which mosquitoes are developing resistance. Viruses utilize the host metabolome for replication and dissemination. This is particularly true for envelope viruses like DENV that relies on host lipid membranes to complete their life-cycle. To reach an optimal…

Advisors/Committee Members: Deharo, Éric (thesis director).

Subjects/Keywords: Dengue; Virus; Moustique; Aedes; Vecteur; Métabolisme; Lipide; Phospholipide; Dengue; Virus; Mosquito; Aedes; Vector; Metabolism; Lipid; Phospholipid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vial, T. (2020). Le virus de la dengue détourne le métabolisme des phospholipides du moustique pour sa réplication : Dengue virus diverts the mosquito phospholipid metabolism for replication. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2020TOU30036

Chicago Manual of Style (16^th Edition):

Vial, Thomas. “Le virus de la dengue détourne le métabolisme des phospholipides du moustique pour sa réplication : Dengue virus diverts the mosquito phospholipid metabolism for replication.” 2020. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed January 20, 2021. http://www.theses.fr/2020TOU30036.

MLA Handbook (7^th Edition):

Vial, Thomas. “Le virus de la dengue détourne le métabolisme des phospholipides du moustique pour sa réplication : Dengue virus diverts the mosquito phospholipid metabolism for replication.” 2020. Web. 20 Jan 2021.

Vancouver:

Vial T. Le virus de la dengue détourne le métabolisme des phospholipides du moustique pour sa réplication : Dengue virus diverts the mosquito phospholipid metabolism for replication. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2020. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2020TOU30036.

Council of Science Editors:

Vial T. Le virus de la dengue détourne le métabolisme des phospholipides du moustique pour sa réplication : Dengue virus diverts the mosquito phospholipid metabolism for replication. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2020. Available from: http://www.theses.fr/2020TOU30036

27. Clain, Marie Élodie. Valorisation des éco-extraits de plantes médicinales réunionnaises dans la lutte contre les maladies virales émergentes de l'océan Indien : Valorization of medicinal plants eco-extracts from La Reunion against emerging viral diseases in the Indian Ocean.

Degree: Docteur es, Biologie médicale, santé, 2018, La Réunion

URL: http://www.theses.fr/2018LARE0033

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Les maladies virales à transmission vectorielle émergentes et ré-émergentes comme la dengue, le chikungunya ou le zika sont responsables de nombreuses épidémies sévères à travers… (more)

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Les maladies virales à transmission vectorielle émergentes et ré-émergentes comme la dengue, le chikungunya ou le zika sont responsables de nombreuses épidémies sévères à travers le monde. Récemment, la propagation rapide et très étendue du virus zika (ZIKV) ainsi que les complications neurologiques graves liées à l’infection par ZIKV ont incité l’Organisation Mondiale de la Santé (OMS) à déclarer le ZIKV comme une urgence de santé publique mondiale. Aujourd’hui, les mesures préventives ou curatives de l’infection par ZIKV sont quasiment inexistantes. D’autre part, la flore endémique de La Réunion est connue comme une source riche, renouvelable et prometteuse en produits naturels anti-infectieux. L’inscription à la pharmacopée française de 19 plantes médicinales réunionnaises souligne le potentiel thérapeutique des substances naturelles issues de la biodiversité locale. Les travaux ont été consacrés à l’identification de substances naturelles anti-ZIKV issues d’une sélection de sept plantes médicinales réunionnaises inscrites à la pharmacopée française. Dans une première étape, une extraction sans solvant assistée par micro-ondes a été appliquée sur les sept plantes médicinales sélectionnées afin d’obtenir des éco-extraits. Dans une deuxième étape, le criblage de l’activité antivirale, en utilisant un clone moléculaire du ZIKV avec un gène rapporteur, a permis d’identifier deux éco-extraits actifs provenant de Aphloia theiformis et de Psiloxylon mauritianum. Après avoir vérifié l’absence de cytotoxicité et de génotoxicité des extraits actifs, l’activité antivirale a été aussi démontrée sur d’autres types d’extraits réalisés via des méthodes d’extractions traditionnelles (infusion, décoction et macération). L’activité antivirale a été validée sur différentes souches de ZIKV (historique et épidémique) ainsi que sur les 4 sérotypes du virus de la dengue. Enfin, le mode d’action des deux extraits actifs a été étudié. Il a été montré que les éco-extraits d’A. theiformis et de P. mauritianum ciblent les phases précoces du cycle viral en inhibant l’attachement du virus à la cellule hôte. À l’aide de la microscopie électronique, il a été montré que l’éco-extrait d’A. theiformis déforme la particule virale empêchant cette dernière de s’attacher à la membrane de la cellule hôte. Ces résultats démontrent l’importance des plantes médicinales réunionnaises comme source de substances naturelles anti-infectieuses.

Emerging and re-emerging vector-borne viral diseases such as dengue, chikungunya or zika are responsible for many severe epidemics worldwide. Recently, the rapid spread of zika virus (ZIKV) worldwide and the serious neurological complications associated with ZIKV infection have prompted the World Health Organization (WHO) to declare ZIKV a public health emergency. Today, preventive or curative measures against ZIKV infection are almost non-existing. On the other hand, the endemic flora of Reunion Island is known as a rich, renewable and promising source of natural anti-infective products. The registration of 19…

Advisors/Committee Members: Guiraud, Pascale (thesis director), El Kalamouni, Chaker (thesis director).

Subjects/Keywords: Virus zika; Flavivirus; Virus de la dengue; Plantes médicinales; Aphloia theiformis; Psiloxylon mauritianum; Activité antivirale; Attachement; Zika virus; Flavivirus; Dengue virus; Medicinal plants; Aphloia theiformis; Psiloxylon mauritianum; Antiviral activity; Attachment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clain, M. E. (2018). Valorisation des éco-extraits de plantes médicinales réunionnaises dans la lutte contre les maladies virales émergentes de l'océan Indien : Valorization of medicinal plants eco-extracts from La Reunion against emerging viral diseases in the Indian Ocean. (Doctoral Dissertation). La Réunion. Retrieved from http://www.theses.fr/2018LARE0033

Chicago Manual of Style (16^th Edition):

Clain, Marie Élodie. “Valorisation des éco-extraits de plantes médicinales réunionnaises dans la lutte contre les maladies virales émergentes de l'océan Indien : Valorization of medicinal plants eco-extracts from La Reunion against emerging viral diseases in the Indian Ocean.” 2018. Doctoral Dissertation, La Réunion. Accessed January 20, 2021. http://www.theses.fr/2018LARE0033.

MLA Handbook (7^th Edition):

Clain, Marie Élodie. “Valorisation des éco-extraits de plantes médicinales réunionnaises dans la lutte contre les maladies virales émergentes de l'océan Indien : Valorization of medicinal plants eco-extracts from La Reunion against emerging viral diseases in the Indian Ocean.” 2018. Web. 20 Jan 2021.

Vancouver:

Clain ME. Valorisation des éco-extraits de plantes médicinales réunionnaises dans la lutte contre les maladies virales émergentes de l'océan Indien : Valorization of medicinal plants eco-extracts from La Reunion against emerging viral diseases in the Indian Ocean. [Internet] [Doctoral dissertation]. La Réunion; 2018. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2018LARE0033.

Council of Science Editors:

Clain ME. Valorisation des éco-extraits de plantes médicinales réunionnaises dans la lutte contre les maladies virales émergentes de l'océan Indien : Valorization of medicinal plants eco-extracts from La Reunion against emerging viral diseases in the Indian Ocean. [Doctoral Dissertation]. La Réunion; 2018. Available from: http://www.theses.fr/2018LARE0033

28. Friberg-Robertson, Heather L. CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation.

Degree: Immunology and Microbiology, Center for Infectious Disease and Vaccine Research, 2010, U of Massachusetts : Med

URL: https://escholarship.umassmed.edu/gsbs_diss/513

► The four serotypes of dengue virus (DENV 1-4) have a significant and growing impact on global health. Dengue disease encompasses a wide range of… (more)

▼ The four serotypes of dengue virus (DENV 1-4) have a significant and growing impact on global health. Dengue disease encompasses a wide range of clinical symptoms, usually presenting as an uncomplicated febrile illness lasting 5-7 days; however, a small percentage of infections are associated with plasma leakage and bleeding tendency (called dengue hemorrhagic fever, DHF), which can result in shock. Epidemiological studies indicate that severe dengue disease most often occurs during secondary heterotypic DENV infection. Additionally, plasma leakage (the hallmark of DHF) coincides with defervescence and viral clearance, suggesting that severe disease arises from the immune response to infection rather than a direct effect of the virus. A number of studies have found increased levels of markers of immune cell activation in patients with DHF compared to patients with the less severe form of disease (DF). These markers include IFNγ, TNFα, soluble CD8, soluble IL-2 receptor, soluble TNF receptor, and CD69, which support a role for T cells in mediating immunopathology. Because of the high homology of DENV 1-4, some degree of serotype-cross-reactivity is seen for most T cell epitopes. A high percentage of DENV-specific T cells recognize multiple DENV serotypes, as demonstrated by peptide-MHC (pMHC) tetramer binding and in vitro functional assays performed on PBMC from subjects vaccinated with an experimental DENV vaccine or naturally-infected subjects with secondary (>1) DENV infection. This thesis sought to address several gaps in the literature, specifically whether T cell responses differ in primary versus secondary (natural) infection. We studied the frequency, phenotype, and function of DENV-specific T cells. We demonstrated substantial serotype-cross-reactivity of antigen-specific T cells generated in response to naturally-acquired primary as well as secondary DENV infection. The frequency of A11-NS3₁₃₃ epitope-specific T cells during acute infection did not correlate with disease severity. However, the peak frequency occurred earlier in primary infection while the frequency of CD45RA⁺ T cells declined quicker in secondary infection, suggesting the expansion of DENV-specific memory T cells. DENV-immune T cells exhibited different functional capabilities that were dependent on the particular serotype of infection. Specifically, DENV-1 or -3 stimulation of A11-NS3₁₃₃ epitope-specific T cell lines resulted in robust function that included IFNγ production, whereas DENV-2 stimulation resulted in limited function that often included MIP-1β but not IFNγ production. These data support a role for T cells in DENV infection and offer new insights into their potential contribution to dengue pathology. Advisors/Committee Members: Alan L. Rothman, M.D..

Subjects/Keywords: Dengue Virus; Dengue; Dengue Hemorrhagic Fever; CD8-Positive T-Lymphocytes; Cross Reactions; Cells; Hemic and Immune Systems; Immunology and Infectious Disease; Pathology; Public Health; Virus Diseases; Viruses

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Friberg-Robertson, H. L. (2010). CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from https://escholarship.umassmed.edu/gsbs_diss/513

Chicago Manual of Style (16^th Edition):

Friberg-Robertson, Heather L. “CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation.” 2010. Doctoral Dissertation, U of Massachusetts : Med. Accessed January 20, 2021. https://escholarship.umassmed.edu/gsbs_diss/513.

MLA Handbook (7^th Edition):

Friberg-Robertson, Heather L. “CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation.” 2010. Web. 20 Jan 2021.

Vancouver:

Friberg-Robertson HL. CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2010. [cited 2021 Jan 20]. Available from: https://escholarship.umassmed.edu/gsbs_diss/513.

Council of Science Editors:

Friberg-Robertson HL. CD8+ T Cell Serotype-Cross-Reactivity is a Predominant Feature of Dengue Virus Infections in Humans: A Dissertation. [Doctoral Dissertation]. U of Massachusetts : Med; 2010. Available from: https://escholarship.umassmed.edu/gsbs_diss/513

Wichita State University

29. Mohan, Swathi. Potential inhibitors of dengue and West Nile virus proteases .

Degree: 2006, Wichita State University

URL: http://hdl.handle.net/10057/569

► The 1,2,5-Thiadiazolidin-3-one 1,1-dioxide scaffold was used in the design and synthesis of inhibitors of Dengue Virus and West Nile Virus proteases and human tryptase. The… (more)

Subjects/Keywords: Dengue virus; West Nile virus; Proteases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mohan, S. (2006). Potential inhibitors of dengue and West Nile virus proteases . (Masters Thesis). Wichita State University. Retrieved from http://hdl.handle.net/10057/569

Chicago Manual of Style (16^th Edition):

Mohan, Swathi. “Potential inhibitors of dengue and West Nile virus proteases .” 2006. Masters Thesis, Wichita State University. Accessed January 20, 2021. http://hdl.handle.net/10057/569.

MLA Handbook (7^th Edition):

Mohan, Swathi. “Potential inhibitors of dengue and West Nile virus proteases .” 2006. Web. 20 Jan 2021.

Vancouver:

Mohan S. Potential inhibitors of dengue and West Nile virus proteases . [Internet] [Masters thesis]. Wichita State University; 2006. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10057/569.

Council of Science Editors:

Mohan S. Potential inhibitors of dengue and West Nile virus proteases . [Masters Thesis]. Wichita State University; 2006. Available from: http://hdl.handle.net/10057/569

University of Melbourne

30. Aktepe, Turgut Esad. Hijacking to use and abuse: the role of host factors during West Nile and Dengue virus replication and membrane mophogenesis.

Degree: 2017, University of Melbourne

URL: http://hdl.handle.net/11343/192290

► Flaviviruses such as West Nile virus and Dengue virus are enveloped, +ssRNA virus from the Flaviviridae family causing infection in North America, Oceania, Africa, Europe,… (more)

▼ Flaviviruses such as West Nile virus and Dengue virus are enveloped, +ssRNA virus from the Flaviviridae family causing infection in North America, Oceania, Africa, Europe, the Middle East and West and central Asia. Due to the limited number of viral proteins encoded by +ssRNA viruses, host factors, such as lipids and proteins, are recruited and required for sufficient viral replication. Host factors are involved in almost every step of +ssRNA virus replication by interacting with viral proteins via a specific motif. It is well established that these viruses manipulate ER homeostasis and distribution to facilitate efficient replication. In this thesis we have investigated the role of the host lipid and protein ceramide and Reticulon 3, respective, alongside the SH3 protein-protein interaction domain. It is well established that +ssRNA viruses manipulate cellular lipid homeostasis and distribution to facilitate efficient replication. Cellular lipid ceramide is redistributed to the West Nile virus strain Kunjin virus (WNVKUN) replication complex (RC) but not to the Dengue virus serotype 2 strain New Guinea C (DENVNGC) RC. Ceramide depletion by prolonged chemical inhibition of serine palmitoyltransferase (SPT) activity with myriocin or inhibition of ceramide synthase with Fumonisin B1 had a significant deleterious effect on WNVKUN replication but enhanced DENVNGC replication. These observations suggest that ceramide production via the de novo and salvage pathway is a requirement for WNVKUN replication but inhibitory for DENVNGC replication. Thus, although these two viruses are from the same genus, they have a differential ceramide requirement for replication. Furthermore, previous studies have shown that a ER membrane-shaping protein Reticulon 3A (RTN3A) plays a crucial role in +ssRNA virus replication by inducing positive-membrane curvature [1, 2]. In this study, we have observed that RTN3A is redistributed and recruited to the RC of WNVKUN and DENVNGC. Subsequent analysis revealed that RTN3A interacts with the viral protein NS4A, known to be responsible for host membrane rearrangements in WNVKUN and DENVNGC infected cells. Via deletion mutagenesis we mapped the interaction between NS4A and RTN3A to the first transmembrane region as removal of the first transmembrane region reduced NS4A – RTN3A interaction by FRET and co-IP experiments. Furthermore, siRNA-mediated knockdown of RTN3A attenuated WNVKUN and DENVNGC viral replication, altered virally induced membrane architecture and severely affected the stability and expression of NS4A. Thus we have demonstrated the importance of RTN3A during Flavivirus replication by interacting with, and stabilizing the NS4A protein. Lastly, we have established that modular interaction domains are crucial during WNV replication. These are generally conserved regions in proteins that are generally 30-200 amino acids. These domains specialize in mediating interactions of proteins with one another, lipids and nucleic acids. Src Homology 3 (SH3) domains are one of the best…

Subjects/Keywords: Flavivirus; West Nile virus; Dengue virus; replication; host interaction; membrane; ceramide; Reticulon; SH3 domain

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aktepe, T. E. (2017). Hijacking to use and abuse: the role of host factors during West Nile and Dengue virus replication and membrane mophogenesis. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/192290

Chicago Manual of Style (16^th Edition):

Aktepe, Turgut Esad. “Hijacking to use and abuse: the role of host factors during West Nile and Dengue virus replication and membrane mophogenesis.” 2017. Doctoral Dissertation, University of Melbourne. Accessed January 20, 2021. http://hdl.handle.net/11343/192290.

MLA Handbook (7^th Edition):

Aktepe, Turgut Esad. “Hijacking to use and abuse: the role of host factors during West Nile and Dengue virus replication and membrane mophogenesis.” 2017. Web. 20 Jan 2021.

Vancouver:

Aktepe TE. Hijacking to use and abuse: the role of host factors during West Nile and Dengue virus replication and membrane mophogenesis. [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11343/192290.

Council of Science Editors:

Aktepe TE. Hijacking to use and abuse: the role of host factors during West Nile and Dengue virus replication and membrane mophogenesis. [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/192290

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Open Access Theses and Dissertations