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You searched for subject:(Dendritic outgrowth). Showing records 1 – 3 of 3 total matches.

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University of Vermont

1. Ni, Xianglian. Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons.

Degree: PhD, Biology, 2009, University of Vermont

Ca2+ influx through ionotropic glutamate receptors regulates a variety of developmental processes including neurite outgrowth and naturally occurring cell death. In the CNS, NMDA receptors were originally thought to be the sole source of Ca2+ influx through glutamate receptors; however, AMPA receptors also allow a significant influx of Ca2+ ions. The Ca2+ permeability of AMPA receptors is regulated by the insertion of one or more edited GluR2 subunits into the receptors. Although Ca2+-permeable AMPA receptors are a familiar feature in developing neurons, the developmental function of these receptors during the formation of the nervous system has yet to be established. This study was designed to investigate the expression and functional role of Ca2+-permeable AMPA receptors in developing chicken spinal motoneurons. Our results demonstrate that chicken lumbar motoneurons express functional AMPA receptors as early as embryonic day (E) 5. Electrophysiological recordings of kainate-evoked currents indicate a significant reduction in the Ca2+ permeability of AMPA receptors between E6 and E11. During this developmental period, the Ca2+ permeability of AMPA receptors decreases three-fold. Reduction in the Ca2+ permeability of AMPA receptors is accompanied by increased expression of GluR2 mRNA in the spinal motoneuron pool. Changes in GluR2 mRNA expression occur in parallel to changes in GluR2 protein expression in the chicken ventral spinal cord. Changes in the Ca2+-permeability of AMPA receptors are not mediated by age-dependent changes in the editing pattern of GluR2 subunits. At early stages of development, functional AMPA receptors were composed of a combination of GluR3 and GluR4 subunits. mRNA analysis indicates that GluR4 is the most abundant subunit in the chicken ventral spinal cord between E6 and E11. Immunohistochemistry analysis of spinal cord sections also demonstrated that both GluR3 and GluR4 proteins are expressed at E6 and E11. Expression of Ca2+-permeable AMPA receptors regulates the maturation of dendritic outgrowth in developing spinal motoneurons. Measurements of dendritic length and branching pattern demonstrate significant changes in the dendritic morphology of spinal motoneurons between E6 and E11. Blockade of AMPA receptor activation with CNQX between E5 and E8 causes a significant increase in dendritic outgrowth in lumbar motoneurons, when compared with vehicle-treated embryos. Treatment of chicken embryos with CNQX between E8 and E11, when AMPA receptors become Ca2+-impermeable, has no affect on dendritic morphology. However, blockade of NMDA receptor activation with MK-801 causes a significant reduction in dendritic outgrowth of lumbar motoneurons by E11. These findings indicate that AMPA receptor activation between E5 and E8 limits dendritic outgrowth in developing motoneurons, whereas NMDA receptor activation is involved in dendritic remodeling after the establishment of synaptic contacts with sensory afferents. Advisors/Committee Members: Martin-Caraballo, Miguel.

Subjects/Keywords: AMPA receptor; Dendritic outgrowth

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ni, X. (2009). Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons. (Doctoral Dissertation). University of Vermont. Retrieved from https://scholarworks.uvm.edu/graddis/161

Chicago Manual of Style (16th Edition):

Ni, Xianglian. “Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons.” 2009. Doctoral Dissertation, University of Vermont. Accessed March 20, 2019. https://scholarworks.uvm.edu/graddis/161.

MLA Handbook (7th Edition):

Ni, Xianglian. “Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons.” 2009. Web. 20 Mar 2019.

Vancouver:

Ni X. Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons. [Internet] [Doctoral dissertation]. University of Vermont; 2009. [cited 2019 Mar 20]. Available from: https://scholarworks.uvm.edu/graddis/161.

Council of Science Editors:

Ni X. Developmental Regulation and Function of AMPA Receptor Subunits in Chicken Lumbar Motoneurons. [Doctoral Dissertation]. University of Vermont; 2009. Available from: https://scholarworks.uvm.edu/graddis/161

2. Nadjar, Yann. Susd2 et Susd4 sont deux nouveaux gènes codant pour des protéines avec domaines CCP (Complement Control Protein) jouant un rôle dans plusieurs étapes du développement des circuits neuronaux au sein de cultures d'hippocampe de rat : Emerging neuronal functions for CCP (Complement Control Protein) containing proteins : characterization of SUSD2 and SUSD4.

Degree: Docteur es, Biologie, 2014, Université Pierre et Marie Curie – Paris VI

Le développement cérébral est une succession d'étapes aboutissant à l'établissement d'un réseau neuronal. Il fait intervenir de nombreuses molécules comme des protéines d'adhésion permettant l'interaction des neurones avec leur environnement. L'implication de nombreux gènes codant des protéines d'adhésion dans la physiopathologie de maladies neuropsychiatriques comme l'autisme souligne l'intérêt à en identifier de nouveaux. Pendant ma thèse, j'ai pu caractériser deux nouveaux gènes, Susd2 et Susd4, codant des protéines contenant des domaines CCP (Complement Control Protein), classiquement connus pour leur présence dans les protéines participant à la régulation du système du Complément. Récemment, des protéines à domaines CCP ont été décrites chez la souris comme ayant une fonction dans le développement neuronal. L'existence de nombreuses protéines prédites à domaines CCP sans fonction connue m'ont conduit à tenter de caractériser Susd2 et Susd4 qui en font partie.Susd2 est exprimé dans les neurones au sein de cultures de cellules d'hippocampe de rat. Son expression atteint un pic à un stade post natal précoce, suggérant une fonction développementale. La protéine Susd2 recombinante a une localisation neuronale diffuse, mais est particulièrement enrichie dans les synapses excitatrices. La diminution de l'expression de Susd2 a pour conséquences un défaut de croissance axonale, une augmentation de la croissance dendritique, et une inhibition spécifique de la synaptogénèse excitatrice. Susd4 est également exprimé dans les neurones, avec un pic d'expression au stade embryonnaire, et semble jouer un rôle de régulation du développement dendritique.

During brain development, several steps precisely coordinated lead to establishment of a functional neuronal network. Many molecules participate to this process, including adhesion proteins mediating interactions between neurons and their environment. Involvement of numerous genes coding for adhesion proteins in neuropsychiatric diseases such as autism argue for usefulness of identifying new ones. During my PhD, I characterized two new genes, Sud2 and Susd4, coding for proteins containing CCP domains (Complement Control Protein), classically described in proteins involved in Complement regulation system. Recently, in mammals, CCP containing proteins were shown to be involved in neuronal development. Identification of several predicted CCP containing proteins without a known function prompted me to characterize Susd2 and Susd4 which are part of them.Susd2 is expressed in neurons from hippocampal cell cultures. Its peak of expression takes place in early post natal period, suggesting a developmental function. Susd2 recombinant protein has a diffuse neuronal localization, but is particularly enriched in excitatory synapses. Decreased expression of Susd2 leads to decreased axonal growth, increased dendritic growth, and specific inhibition of excitatory synaptogenesis. Susd4 is also expressed in neurons, with a peak of expression during embryonic development, and seems to act as a…

Advisors/Committee Members: Marty, Serge (thesis director).

Subjects/Keywords: Domaines CCP; Synaptogénèse; Croissance axonale; Croissance dendritique; Protéines d'adhésion; Autisme; CCP domains; Dendritic outgrowth; 573.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Nadjar, Y. (2014). Susd2 et Susd4 sont deux nouveaux gènes codant pour des protéines avec domaines CCP (Complement Control Protein) jouant un rôle dans plusieurs étapes du développement des circuits neuronaux au sein de cultures d'hippocampe de rat : Emerging neuronal functions for CCP (Complement Control Protein) containing proteins : characterization of SUSD2 and SUSD4. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2014PA066664

Chicago Manual of Style (16th Edition):

Nadjar, Yann. “Susd2 et Susd4 sont deux nouveaux gènes codant pour des protéines avec domaines CCP (Complement Control Protein) jouant un rôle dans plusieurs étapes du développement des circuits neuronaux au sein de cultures d'hippocampe de rat : Emerging neuronal functions for CCP (Complement Control Protein) containing proteins : characterization of SUSD2 and SUSD4.” 2014. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed March 20, 2019. http://www.theses.fr/2014PA066664.

MLA Handbook (7th Edition):

Nadjar, Yann. “Susd2 et Susd4 sont deux nouveaux gènes codant pour des protéines avec domaines CCP (Complement Control Protein) jouant un rôle dans plusieurs étapes du développement des circuits neuronaux au sein de cultures d'hippocampe de rat : Emerging neuronal functions for CCP (Complement Control Protein) containing proteins : characterization of SUSD2 and SUSD4.” 2014. Web. 20 Mar 2019.

Vancouver:

Nadjar Y. Susd2 et Susd4 sont deux nouveaux gènes codant pour des protéines avec domaines CCP (Complement Control Protein) jouant un rôle dans plusieurs étapes du développement des circuits neuronaux au sein de cultures d'hippocampe de rat : Emerging neuronal functions for CCP (Complement Control Protein) containing proteins : characterization of SUSD2 and SUSD4. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. [cited 2019 Mar 20]. Available from: http://www.theses.fr/2014PA066664.

Council of Science Editors:

Nadjar Y. Susd2 et Susd4 sont deux nouveaux gènes codant pour des protéines avec domaines CCP (Complement Control Protein) jouant un rôle dans plusieurs étapes du développement des circuits neuronaux au sein de cultures d'hippocampe de rat : Emerging neuronal functions for CCP (Complement Control Protein) containing proteins : characterization of SUSD2 and SUSD4. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. Available from: http://www.theses.fr/2014PA066664


University of South Florida

3. Hamel, Michelle Grace. Modulation of neural plasticity by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs).

Degree: 2006, University of South Florida

Aggregating proteoglycans (PG) bearing chondroitin sulfate (CS) side chains are well-known inhibitors of neural plasticity and associate with hyaluronan and tenascin-R to form a complex of extracellular matrix (ECM) in the central nervous system (CNS). Little is known about whether proteolytic cleavage of the core protein affects neural plasticity. Several members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of metalloproteinases are glutamyl-endopeptidases that cleave aggregating PGs. Our initial studies determined that neural cultures secrete a brevican-containing matrix, and that these neural cultures also produced ADAMTS4, a protease that cleaves brevican. Furthermore, this brevican-containing matrix in astrocytes could be modulated by treatment with transforming growth factor beta (TGFbeta) through the inhibition of the activity of the ADAMTSs.Once it was established that neural cultures produce a brevican-rich matrix, we s ought to utilize this matrix to determine whether cleavage of aggregating PGs, especially brevican, by the ADAMTSs influences neurite outgrowth in cultured neurons. Transfection of rat neurons with ADAMTS4 cDNA induced longer neurites, and interestingly, this effect proved to be independent of the proteolytic action of the ADAMTSs. Addition of recombinant ADAMTS4 or ADAMTS5 protein to immature neuronal cultures similarly enhanced neurite extension, an action dependent on the activation of extracellular signal-related kinase (ERK)1/2 (MAP kinase 42/44), resulting in the first evidence that ADAMTSs may induce intracellular signaling events. Studies of dendritic spine morphology and levels of synaptic proteins in response to ADAMTS4 treatment were also undertaken. Neuronal cultures treated with ADAMTS4 showed increased length of dendritic spines and increased percent of immature spines detected. A concurrent decrease in post-synaptic protein staining was detected on the neurites of yo ung neurons overexpressing ADAMTS4 or expressing proteolytically-inactive mutant ADAMTS4 protein. Thus, ADAMTS4 may promote plasticity in neurons in vitro by preventing the formation, maturation, and/or stabilization of synapses. Overall, these experiments provide evidence that implicate the ADAMTSs as mediators of neural plasticity, and while primarily known only as proteases, these studies demonstrate that the ADAMTSs exert actions distinct from these proteolytic properties that require the induction of intracellular signaling events.

Subjects/Keywords: Neurite outgrowth; Dendritic spine; Proteoglycan; Extracellular matrix; Protease; American Studies; Arts and Humanities

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hamel, M. G. (2006). Modulation of neural plasticity by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs). (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/2544

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Hamel, Michelle Grace. “Modulation of neural plasticity by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs).” 2006. Thesis, University of South Florida. Accessed March 20, 2019. https://scholarcommons.usf.edu/etd/2544.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Hamel, Michelle Grace. “Modulation of neural plasticity by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs).” 2006. Web. 20 Mar 2019.

Vancouver:

Hamel MG. Modulation of neural plasticity by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs). [Internet] [Thesis]. University of South Florida; 2006. [cited 2019 Mar 20]. Available from: https://scholarcommons.usf.edu/etd/2544.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hamel MG. Modulation of neural plasticity by the ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs). [Thesis]. University of South Florida; 2006. Available from: https://scholarcommons.usf.edu/etd/2544

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.